| 1. |
Lateral Diffusion of a Probe Lipid in Biphasic Phospholipid Monolayers: Liquid/Gas Coexistence Films
The method of fluorescence recovery after photobleaching has been used to determine the lateral diffusion coefficient (D) of a fluorescent lipid probe in L-α-dilauroylphosphatidylcholine monolayers. The surface mass density was varied over a wide range from the liquid expanded/gas coexistence phase (LE/G) to the homogeneous liquid phase. The lateral diffusion coefficient reached a maximum value at the “liftoff” point on the surface pressure-area curve (D ≈ 1.2 × 1(10 −10 m 2 /s, A = 1.1−1.3 nm 2 /molecule, T = 22−24 °C), corresponding to the onset of the uniphasic LE state, and decreased from this maximum as the mass density was varied to both higher and lower values of area per molecule (A). The results were analyzed according to the effective-medium theory and the free area model, respectively. The D values in the LE/G biphasic monolayers were evaluated as a function of the area fraction of the LE phase, whereby it was shown that the gas bubbles in the LE/G can be regarded as semipermeable obstacles for the lipid probes, with a relative permeability of the gas bubbles with respect to the LE phase of 0.2–0.3. © 1993, American Chemical Society. All rights reserved.. |
| 2. |
Y Hoshino, S Tajima, H Nakayama, Y Okahata, RNA-aligned film prepared from an RNA/lipid complex, MACROMOLECULAR RAPID COMMUNICATIONS, 10.1002/1521-3927(20020301)23:43.0.CO;2-H, Vol.23, No.4, pp.253-255, 2002.03, Communication: Strong films were prepared from an RNA/lipid complex by casting from organic solutions. RNA (mainly tRNA) was extracted from yeasts, followed by a replacement of the sodium counterions of the tRNA phosphate units by cationic amphiphiles. RNA units in the RNA/lipid film could be aligned in one direction by stretching due to the presence of inter-molecular hydrogen bonds. The film showed physical strength and was biodegradable.. |
| 3. |
Yu Hoshino, Sachiko Tajima, Hajime Nakayama, Yoshio Okahata, RNA-aligned film prepared from an RNA/lipid complex, Macromolecular Rapid Communications, 10.1002/1521-3927(20020301)23:43.0.CO;2-H, Vol.23, No.4, pp.253-255, 2002.03, Strong films were prepared from an RNA/lipid complex by casting from organic solutions. RNA (mainly tRNA) was extracted from yeasts, followed by a replacement of the sodium counterions of the tRNA phosphate units by cationic amphiphiles. RNA units in the RNA/lipid film could be aligned in one direction by stretching due to the presence of intermolecular hydrogen bonds. The film showed physical strength and was biodegradable.. |
| 4. |
T Kawasaki, Y Hoshino, Y Ishizu, Y Mizushiro, Y Okahata, Control of hydrolysis and condensation activities of thermolysin by ultrasound irradiation, CHEMISTRY LETTERS, 10.1246/cl.2005.1602, Vol.34, No.12, pp.1602-1603, 2005.12, Hydrolysis and condensation reactions of peptides catalyzed by thermolysin can be reversibly controlled by on/off ultrasound irradiation depending on its frequency region.. |
| 5. |
Y Hoshino, T Kawasaki, Y Okahata, Effect of ultrasound on DNA polymerase reactions: Monitoring on a 27-MHz quartz crystal microbalance, BIOMACROMOLECULES, 10.1021/bm050738e, Vol.7, No.3, pp.682-685, 2006.03, Effects of ultrasound irradiation on DNA polymerase (Klenow fragment, KF) reactions were studied on the template/ primer DNA-immobilized quartz crystal microbalance (QCM). Under ultrasound irradiation, binding of KF to the DNA was suppressed due to the decrease of the binding rate constant (k(1)) and the increase of the dissociation rate constant (k(-1)). The catalytic elongation rate (k(cat)) was increased, but the stability of the KF/DNA/monomer ternary complex (K-m) was decreased by the ultrasound irradiation. Ultrasound effects are discussed in correlation with the conformation changes of domain structures in KF.. |
| 6. |
Yu Hoshino, Takashi Kodama, Yoshio Okahata, Kenneth J. Shea, Peptide Imprinted Polymer Nanoparticles: A Plastic Antibody, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 10.1021/ja8062875, Vol.130, No.46, pp.15242-+, 2008.11, A novel method for preparation of biomacromolecular imprinted nanoparticles is described. Combinations of functional monomers were polymerized in the presence of the imprinting peptide melittin in aqueous solution at room temperature to produce a small library of polymer nanoparticles. The template peptide and unreacted monomers are subsequently removed by dialysis. Nanoparticles (NPs) from the library were evaluated for their binding to melittin by 27 MHz QCM analysis. NPs prepared with optimized functional monomer combinations bind strongly to the target molecule. Nanoparticles that were polymerized in the absence of template peptide were found to have little affinity to the peptide. Binding affinity and the size of imprinted particles are comparable to those of natural antibodies. They interact specifically with the target peptide and show little affinity for other proteins. These NPs are of interest as inert and stable substitutes for antibodies. Extension of this approach to other targets of biological importance and the applications of these materials are currently being evaluated.. |
| 7. |
Takayoshi Kawasaki, Momoko Toyoda, Yu Hoshino, Yoshio Okahata, Pulsed Ultrasound Effect on DNA Polymerase Reaction Monitored on a QCM, CHEMISTRY LETTERS, 10.1246/cl.2009.538, Vol.38, No.6, pp.538-539, 2009.06, DNA polymerase reaction under pulsed ultrasound irradiation was investigated on a DNA-immobilized quartz-crystal microbalance QCM), and the 240-kHz ultrasound irradiation pulsed at 20s(-1) increased the polymerization reactivity owing to the increase of the stability of the DNA/polymerase/monomer complex, although the continuous irradiation decreases the stability.. |
| 8. |
Yu Hoshino, Takeo Urakami, Takashi Kodama, Hiroyuki Koide, Naoto Oku, Yoshio Okahata, Kenneth J. Shea, Design of Synthetic Polymer Nanoparticles that Capture and Neutralize a Toxic Peptide, SMALL, 10.1002/smll.200900186, Vol.5, No.13, pp.1562-1568, 2009.07, Designed polymer nanoparticles (NPs) capable of binding and neutralizing a biomacromolecular toxin are prepared. A library of copolymer NPs is synthesized from combinations of functional monomers. The binding capacity and affinity of the NPs are individually analyzed. NPs with optimized composition are capable of neutralizing the toxin even in a complex biological milieu. It is anticipated that this strategy will be a starting point for the design of synthetic alternatives to antibodies.. |
| 9. |
Yu Hoshino, Hiroyuki Koide, Takashi Kodama, Takeo Urakami, Naoto Oku, Yoshio Okahata, Kenneth J. Shea, COLL 273-Design of polymer nanoparticles to capture and neutralize the toxic peptide melittin, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.238, 2009.08. |
| 10. |
Zhiyang Zeng, Yu Hoshino, Andy Rodriguez, Hoseong Yoo, Kenneth J. Shea, Synthetic Polymer Nanoparticles with Antibody-like Affinity for a Hydrophilic Peptide, ACS NANO, 10.1021/nn9011256s, Vol.4, No.1, pp.199-204, 2010.01, Synthetic polymer nanoparticles with antibody-like affinity for a hydrophilic peptide have been prepared by inverse microemulsion polymerization. Peptide affinity was achieved in part by incorporating the target (imprint) peptide in the polymerization reaction mixture. Incorporation of the imprint peptide assists in the creation of complementary binding sites in the resulting polymer nanoparticle (NP). To orient the imprint peptide at the interface of the water and oil domains during polymerization, the peptide target was coupled with fatty acid chains of varying length. The peptide-NP binding affinities (ca. 90-900 nM) were quantitatively evaluated by a quartz crystal microbalance (QCM). The optimal chain length was established that created high affinity peptide binding sites on the surface of the nanoparticles. This method can be used for the preparation of nanosized synthetic polymers with antibody-like affinity for hydrophilic peptides and proteins ("plastic antibodies").. |
| 11. |
Yu Hoshino, Hiroyuki Koide, Takeo Urakami, Hiroaki Kanazawa, Takashi Kodama, Naoto Oku, Shea J. Kenneth, Recognition and neutralization of a toxic peptide in vivo by polymer nanoparticles, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.239, 2010.03. |
| 12. |
Yu Hoshino, Takashi Kodama, Yoshio Okahata, Kenneth J. Shea, Affinity separation of stimuli responsive peptide-receptor nanoparticles: Towards a monoclonal "plastic" antibody, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.239, 2010.03. |
| 13. |
Shih-Hui Lee, Yu Hoshino, Ruey-an Doong, Kenneth J. Shea, Development of plastic nanoparticles that capture antibodies: Plastic protein A, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.241, 2011.03. |
| 14. |
Yu Hoshino, Hiroyuki Koide, Dai Oyama, Yusuke Yonamine, Shih-Hui Lee, Naoto Oku, Kenneth J. Shea, Design of polymer nanoparticles that are capable of neutralizing toxicity of fetal proteins, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.241, 2011.03. |
| 15. |
Keiichi Yoshimatsu, Benjamin K. Lesel, Yu Hoshino, Kenneth J. Shea, Selective protein capture using designed synthetic polymer nanoparticles, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.243, 2012.03. |
| 16. |
Yu Hoshino, Masahiko Nakamoto, Yoshiko Miura, Influence of polymer density of plastic antibodies on target binding kinetics, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.243, 2012.03. |
| 17. |
Keiichi Yoshimatsu, Benjamin K. Lesel, Yu Hoshino, Kenneth J. Shea, Designed synthetic polymer nanoparticles for capturing of proteins, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.243, 2012.03. |
| 18. |
Ryohei Ohashi, Yu Hoshino, Yoshiko Miura, Reversible absorption of protons by temperature-responsive gel-particles, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.245, 2013.04. |
| 19. |
Yu Hoshino, Kazushi Imamura, Mengchen Yue, Yoshiko Miura, Reversible absorption of CO2 by aqueous solution of amine-containing poly-N-isopropylacrylamide particles, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.245, 2013.04. |
| 20. |
Hiroyuki Koide, Yu Hoshino, Yuri Nishimura, Yoshiko Miura, Naoto Oku, Kenneth J. Shea, Synthetic polymer nanoparticle with engineered affinity for a vascular endothelial growth factor (VEGF), ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.248, 2014.08. |
| 21. |
Yoke Ming Wong, Yu Hoshino, Kumar Sudesh, Yoshiko Miura, Keiji Numata, Enzyme-like amidolytic catalysis from optimized poly(N-isopropylacrylamide) microgel, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.248, 2014.08. |
| 22. |
Yu Hoshino, Ryohei Ohashi, Yoshiko Miura, Development of liquid membranes consisting of aqueous solution of proton-imprinted nanogel particles for heat-induced ion separation, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.248, 2014.08. |
| 23. |
Tatsuya Fukuta, Hiroyuki Koide, Yu Hoshino, Yoshiko Miura, Kenneth J. Shea, Naoto Oku, Development of anti-high mobility group box-1 synthetic polymer nanoparticles for the treatment of cerebral ischemia-reperfusion injury, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.248, 2014.08. |
| 24. |
Masahiko Nakamoto, Yu Hoshino, Yoshiko Miura, Correlation between protein binding process of nanogel particles and facilitation of protein refolding, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.248, 2014.08. |
| 25. |
Yu Hoshino, Tomohiro Gyobu, Ryutaro Honda, Kazuki Imamura, Chie Yamashita, Takeshi Watanabe, Ikuo Taniguchi, Yoshiko Miura, Preparation of CO2 permeable nanomembranes from amine-containing nanogelparticles, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.253, 2017.04. |
| 26. |
Yusuke Yonamine, Yuta Suzuki, Takuro Ito, Yoshiko Miura, Yasuyuki Ozeki, Keisuke Goda, Yu Hoshino, Monitoring photosynthetic metabolism in a microalgal cell by Raman spectroscopy with stable isotope labeling, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.255, 2018.03. |
| 27. |
Hiroyuki Koide, Yu Hoshino, Yuri Nishimura, Yoshiko Miura, Naoto Oku, Kenneth Shea, Tomohiro Asai, Development of a plastic antibody that captures vascular endothelial growth factor (VEGF) for anticancer therapy, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol.258, 2019.08. |
| 28. |
Y. Miura, T. Fukuda, H. Seto, Y. Hoshino, Development of glycosaminoglycan mimetics using glycopolymers, Polym. J., 2015.11. |
| 29. |
Y. Miura, Y. Hoshino, H. Seto, Glycopolymer Nanobiotechnology, Chem. Rev., 2015.10. |
| 30. |
星野 友, Proton Imprinted Nanoparticles: A Mimic of Enzymes, 高分子学会 会誌「高分子」“Hot Topics”9月号, 2013.09. |
