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Akihito Harada
, Epigenomic lineage tracing toward the understanding acquisition of tissue-specific gene expression profile
, 理研生命医科学研究センター(IMS), 2021.09. |
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Akihito Harada, Kazumitsu Maehara, Yasuyuki Ohkawa, High-throughput single cell epigenomic profiling using chromatin integration labelling-sequence, Keystone Symposia on Molecular and Cellular Biology, 2019.03, Analysis of chromatin states is regularly performed in development and disease research of multicellular organisms. The standard method of epigenomic analysis has been chromatin immunoprecipitation followed by sequencing (ChIP-seq) which is a powerful technique capable of unveiling protein-DNA interactions on the whole genome. There has been a recent development of the method allowing sequencing from single cells, which could facilitate the analysis of clinical samples as well as rare cell populations such as stem cells. However, the single-cell ChIP protocol employs microfluidic devices and hence still requires a great number of cells. Recently, we have established a new immunoprecipitation-free epigenomic profiling method based on immunostaining named chromatin integration labeling followed by sequencing (ChIL-seq). While ChIL-seq was demonstrated to detect histone modifications at the single-cell level, the current published protocol is not cost-effective for high-throughput assays because all the reactions need to be performed separately. We report on our progress on the further development of single-cell ChIL-seq at high-throughput.. |
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Harada A, Histone H3.3 sub-variant H3mm7 is required for normal skeletal muscle regeneration., 1st München-Japan Mini Symposium “Chromatin Structure and Function”, 2017.09. |
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Harada A, Functional analysis of novel histone variant H3mm13 in skeletal muscle to Histone variants, EMBO Workshop Histone Variants, 2017.06. |
