九州大学 研究者情報
論文一覧
工藤 奨(くどう すすむ) データ更新日:2019.06.26

教授 /  工学研究院 機械工学部門 生体工学


原著論文
1. Toshihiro Sera, Masataka Arai, Zhonghua Cui, Koichi Onose, Alireza Karimi, Susumu Kudo, Unloading of intercellular tension induces the directional translocation of PKCα, Journal of cellular physiology, 10.1002/jcp.27662, 234, 6, 9764-9777, 2019.06, [URL], The migration of endothelial cells (ECs) is closely associated with a Ca
2+
-dependent protein, protein kinase Cα (PKCα). The disruption of intercellular adhesion by single-cell wounding has been shown to induce the directional translocation of PKCα. We hypothesized that this translocation of PKCα is induced by mechanical stress, such as unloading of intercellular tension, or by intercellular communication, such as gap junction-mediated and paracrine signaling. In the current study, we found that the disruption of intercellular adhesion induced the directional translocation of PKCα even when gap junction-mediated and paracrine signaling were inhibited. Conversely, it did not occur when the mechanosensitive channel was inhibited. In addition, the strain field of substrate attributable to the disruption of intercellular adhesion tended to be larger at the areas corresponding with PKCα translocation. Recently, we found that a direct mechanical stimulus induced the accumulation of PKCα at the stimulus area, involving Ca
2+
influx from extracellular space. These results indicated that the unloading of intercellular tension induced directional translocation of PKCα, which required Ca
2+
influx from extracellular space. The results of this study indicate the involvement of PKCα in the Ca
2+
signaling pathway in response to mechanical stress in ECs..
2. Toshihiro Sera, Keiichi Ueyama, Alireza Karimi, Susumu Kudo, The effects of fluid shear stress and O
2
concentration on the phosphorylation of eNOS at Ser
635
in endothelial cells, Synergy, 10.1016/j.synres.2019.100046, 8, 2019.06, [URL], Under hypoxic conditions, NO plays an important role in regulating O
2
delivery by controlling local blood vessel relaxation. NO is primarily produced by endothelial NO synthase (eNOS), which is reportedly phosphorylated at Ser
635
and thereby activated under conditions of fluid shear stress and O
2
concentration. The aim of this work was to investigate the effects of fluid shear stress and O
2
concentration on the phosphorylation of eNOS at Ser
635
. Bovine aortic endothelial cells were exposed to hypoxia only, a combination of shear stress and hyperoxia, and a combination of shear stress and hypoxia at various time points. Hypoxia did not increase phosphorylation significantly at 15 min but induced a gradual increase to 1.94-fold over 180 min. Under simultaneous exposures to shear stress and hyperoxia, eNOS phosphorylation was detected after 15-min and was 2.75-fold higher than the initial condition at the 60-min time point. In contrast, under a combination of shear stress and hypoxia, eNOS phosphorylation was increased to 2.44-fold at 60 min. However, although phosphorylation levels under these conditions were higher than those after hypoxia only at all time points, they were lower than those after a combination of shear stress and hyperoxia. Our results indicate that hyperoxia and shear stress additively stimulate phosphorylation of eNOS at Ser
635
and suggest a moderating role of hypoxia..
3. Alireza Karimi, Reza Razaghi, Toshihiro Sera, Susumu Kudo, A combination of the finite element analysis and experimental indentation via the cornea, Journal of the Mechanical Behavior of Biomedical Materials, 10.1016/j.jmbbm.2018.10.017, 90, 146-154, 2019.02, [URL], The purpose of this study was to perform a set of experimental indentation test to certify our proposed eye model enables to have a better deformation assessment for the eye globe under the indentation load compared to other eye models. To do that, twenty-four enucleated human globes were removed from the cadavers. A screw at 5 different loading rates indented to the eye globes and the resulting macroscopic force-displacement as a result of the deformation in the apex of the cornea was measured. The experimental results revealed significantly higher stiffness, elastic modulus, and maximum force for the globe at higher loading rates (50 and 100 mm/min) (n = 4 globes, p < 0.05, post hoc Scheffe method) compared to the lower ones (5, 10, and 20 mm/min). The mean stiffness, elastic modulus as well as the mean maximum force of 1.64 ± 0.38 N/mm, 303.40 ± 111.10 kPa, and 53.88 ± 17.63 N (Mean ± SD) were observed for the eye globes under all loading rates, respectively. Our eye model due to incorporating the anterior (cornea, aqueous body, and iris) and posterior (sclera, retina, and vitreous body) components showed a good agreement with force-displacement diagrams compared to that of the experimental results not only at lower but also at higher loading rates..
4. Toshihiro Sera, Shingo Komine, Masataka Arai, Yasuhiro Sunaga, Hideo Yokota, Susumu Kudo, Three-dimensional model of intracellular and intercellular Ca2+ waves propagation in endothelial cells, Biochemical and Biophysical Research Communications, 10.1016/j.bbrc.2018.10.001, 505, 3, 781-786, 2018.11, [URL], Intracellular and intercellular Ca2+ waves play key roles in cellular functions, and focal stimulation triggers Ca2+ wave propagation from stimulation points to neighboring cells, involving localized metabolism reactions and specific diffusion processes. Among these, inositol 1,4,5-trisphosphate (IP3) is produced at membranes and diffuses into the cytoplasm to release Ca2+ from endoplasmic reticulum (ER). In this study, we developed a three-dimensional (3D) simulation model for intercellular and intracellular Ca2+ waves in endothelial cells (ECs). 3D model of 2 cells was reconstructed from confocal microscopic images and was connected via gap junctions. Cells have membrane and cytoplasm domains, and metabolic reactions were divided into each domain. Finally, the intracellular and intercellular Ca2+ wave propagations were induced using microscopic stimulation and were compared between numerical simulations and experiments. The experiments showed that initial sharp increases in intracellular Ca2+ occurred approximately 0.3 s after application of stimuli. In addition, Ca2+ wave speeds remained constant in cells, with intracellular and intercellular speeds of approximately 35 and 15 μm/s, respectively. Simulations indicated initial increases in Ca2+ concentrations at points of stimulation, and these were then propagated across stimulated and neighboring cells. In particular, initial rapid increases in intracellular Ca2+ were delayed and subsequent intracellular and intercellular Ca2+ wave speeds were approximately 25 and 12 μm/s, respectively. Simulation results were in agreement with those from cell culture experiments, indicating the utility of our 3D model for investigations of intracellular and intercellular messaging in ECs..
5. Alireza Karimi, Reza Razaghi, Seyed Mohammadali Rahmati, Toshihiro Sera, Susumu Kudo, A nonlinear dynamic finite-element analyses of the basketball-related eye injuries, Sports Engineering, 10.1007/s12283-018-0280-3, 21, 4, 359-365, 2018.12, [URL], Eye injuries from blunt forces and penetrating objects can cause morbidity, disability, and blindness. Most of these injuries are preventable not only by understanding the detail of injury, but also by portraying the role of the ball’s characteristics. Basketball can induce severe forms of injuries to the eye. This study was aimed at performing a numerical analysis through the fluid–structure interaction on ocular trauma. Balls with different diameters, elastic moduli, and pressures were shot to the eye. Injury in terms of the stresses and deformations in each component of the eye was computed at ~ 0.72 mm deformation in the apex of the cornea. By increasing the diameter of the ball from 20 to 26 cm the stress in the eye components, excluding the aqueous body, vitreous body, and extraconal fat, increased. Stresses of 2.68 and 3.19 MPa were observed in the cornea under the ball diameters of 20 and 26 cm, respectively. Conversely, the elastic modulus and pressure of the ball in the defined range showed no considerable role on the stress in the eye components. The results revealed the importance of the ball size compared to the elastic modulus and pressure in the stress accumulation as a result of ocular trauma..
6. Alireza Karimi, Reza Razaghi, Hasan Biglari, Toshihiro Sera, Susumu Kudo, Collision of the glass shards with the eye
A computational fluid-structure interaction model, Journal of Chemical Neuroanatomy, 10.1016/j.jchemneu.2017.12.008, 90, 80-86, 2018.07, [URL], The main stream of blunt trauma injuries has been reported to be related to the automobile crashes, sporting activities, and military operations. Glass shards, which can be induced due to car accident, earthquake, gunshot, etc., might collide with the eye and trigger substantial scarring and, consequently, permanently affect the vision. The complications as a result of the collision with the eye and its following injuries on each component of the eye are difficult to be diagnosed. The objective of this study was to employ a Three-Dimensional (3D) computational Fluid-Structure Interaction (FSI) model of the human eye to assess the results of the glass shards collision with the eye. To do this, a rigid steel-based object hit a Smoothed-Particle Hydrodynamics (SPH) glass wall at the velocities of 100, 150, and 200 m/s and, subsequently, the resultant glass shards moved toward the eye. The amount of injury, then, quantified in terms of the stresses and strains. The results revealed the highest amount of stress in the cornea while the lowest one was observed in the vitreous body. It was also found that increasing the speed of the glass shards amplifies the amount of the stress in the components which are located in the central anterior zone of the eye, such as the cornea, aqueous body, and iris. However, regarding those components located in the peripheral/posterior side of the eye, especially the optic nerve, by increasing the amount of velocity a reduction in the stresses was observed and the optic nerve is hardly damaged. These findings have associations not only for understanding the amount of stresses/strains in the eye components at three different velocities, but also for providing preliminary information for the ophthalmologists to have a better diagnosis after glass shards (small objects impact) injuries to the eye..
7. Masataka Arai, Toshihiro Sera, Takumi Hasegawa, Susumu Kudo, Spatial and temporal translocation of PKCα in single endothelial cell in response to mechanical stimulus, Experimental Cell Research, 10.1016/j.yexcr.2018.03.038, 367, 2, 205-215, 2018.06, [URL], Endothelial cells (ECs) are exposed to various environmental forces, and a Ca2+ wave is occurred in mechanical stimulated cells. Pharmacological studies reveal that the translocation of protein kinase Cα (PKCα) to the membrane is observed simultaneously with intracellular Ca2+ wave. In this study, we investigate whether and how the kinetics of PKCα in ECs is induced in response to mechanical stress. The results show that a mechanical stimulus induced biphasic and directional PKCα translocation; PKCα initially translocated near or at the membrane and then accumulated at the stimulus point. The initial translocation occurred simultaneously with Ca2+ increase. Initial translocation was inhibited in spite of Ca2+ increase when the diacylglycerol (DAG) binding domain of PKCα was inhibited, suggesting that translocation requires intracellular Ca2+ increase and DAG. On the other hand, secondary translocation was delayed, occurring after the Ca2+ wave; however, this translocation occurred even when Ca2+ release from the endoplasmic reticulum was inhibited, while it did not occur when the mechanosensitive (MS) channel was inhibited. These results indicated that at least Ca2+ influx from extracellular space through MS channel is required. Our results support the implication of PKCα in the Ca2+ signaling pathway in response to mechanical stress in ECs..
8. Toshihiro Sera, Tateki Sumii, Ryosuke Fujita, Susumu Kudo, Effect of shear stress on the migration of hepatic stellate cells, In Vitro Cellular and Developmental Biology - Animal, 10.1007/s11626-017-0202-x, 54, 1, 11-22, 2018.01, [URL], When the liver is damaged, hepatic stellate cells (HSCs) can change into an activated, highly migratory state. The migration of HSCs may be affected by shear stress due not only to sinusoidal flow but also by the flow in the space of Disse because this space is filled with blood plasma. In this study, we evaluated the effects of shear stress on HSC migration in a scratch-wound assay with a parallel flow chamber. At regions upstream of the wound area, the migration was inhibited by 0.6 Pa and promoted by 2.0 Pa shear stress, compared to the static condition. The platelet-derived growth factor (PDGF)-BB receptor, PDGFR-β, was expressed in all conditions and the differences were not significant. PDGF increased HSC migration, except at 0.6 Pa shear stress, which was still inhibited. These results indicate that another molecular factor, such as PDGFR-α, may act to inhibit the migration under low shear stress. At regions downstream of the wound area, the migration was smaller under shear stress than under the static condition, although the expression of PDGFR-β was significantly higher. In particular, the migration direction was opposite to the wound area under high shear stress; therefore, migration might be influenced by the intercellular environment. Our results indicate that HSC migration was influenced by shear stress intensity and the intercellular environment..
9. Masataka Arai, Tomoya Shimada, Chihiro Kora, Kazuhiro Nakashima, Toshihiro Sera, Susumu Kudo, Biphasic and directed translocation of protein kinase Cα inside cultured endothelial cells before migration, Biochemistry and Biophysics Reports, 10.1016/j.bbrep.2017.08.003, 12, 91-97, 2017.12, [URL], Mechanical wounding of an endothelial monolayer induces an immediate Ca2+ wave. Several hours later, the denuded area is covered by endothelial cells (ECs) that migrate to the wound. This migration process is closely related to protein kinase Cα (PKCα), a Ca2+-dependent protein that translocates from the cytosol to the cell membrane. Because the cells adjacent to the wounded area are the first to migrate into the wound, we investigated whether a mechanical wound immediately induces PKCα translocation in adjacent cells. We monitored Ca2+ dynamics and PKCα translocation simultaneously using fluorescent microscopy. For this simultaneous observation, we used Fura-2–acetoxymethyl ester to visualize Ca2+ and constructed a green fluorescent protein-tagged fusion protein to visualize PKCα. Mechanical wounding of the endothelial monolayer induced an immediate Ca2+ wave in cells adjacent to the wounded cells before their migration. Almost concurrently, PKCα in the neighboring cells translocated to the cell membrane, then accumulated at the periphery near the wounded cell. This report is the first description of this biphasic and directed translocation of PKCα in cells before cell migration. Our results may provide new insights into the directed migration of ECs..
10. Alireza Karimi, Seyed Mohammadali Rahmati, Toshihiro Sera, Susumu Kudo, Mahdi Navidbakhsh, A Combination of Constitutive Damage Model and Artificial Neural Networks to Characterize the Mechanical Properties of the Healthy and Atherosclerotic Human Coronary Arteries, Artificial Organs, 10.1111/aor.12855, 41, 9, E103-E117, 2017.09, [URL], It has been indicated that the content and structure of the elastin and collagen of the arterial wall can subject to a significant alteration due to the atherosclerosis. Consequently, a high tissue stiffness, stress, and even damage/rupture are triggered in the arterial wall. Although many studies so far have been conducted to quantify the mechanical properties of the coronary arteries, none of them consider the role of collagen damage of the healthy and atherosclerotic human coronary arterial walls. Recently, a fiber family-based constitutive equation was proposed to capture the anisotropic mechanical response of the healthy and atherosclerotic human coronary arteries via both the histostructural and uniaxial data. In this study, experimental mechanical measurements along with histological data of the healthy and atherosclerotic arterial walls were employed to determine the constitutive damage parameters and remodeling of the collagen fibers. To do this, the preconditioned arterial tissues were excised from human cadavers within 5-h postmortem, and the mean angle of their collagen fibers was precisely determined. Thereafter, a group of quasistatic axial and circumferential loadings were applied to the arterial walls, and the constrained nonlinear minimization method was employed to identify the arterial parameters according to the axial and circumferential extension data. The remodeling of the collagen fibers during the tensile test was also predicted via Artificial Neural Networks algorithm. Regardless of loading direction, the results presented a noteworthy load-bearing capability and stiffness of the atherosclerotic arteries compared to the healthy ones (P < 0.005). Theoretical fiber angles were found to be consistent with the experimental histological data with less than 2 and 5° difference for the healthy and atherosclerotic arterial walls, respectively. The pseudoelastic damage model data were also compared with that of the experimental data, and interestingly, the arterial mechanical behavior for both the primary loading (up to the elastic region) and the discontinuous softening (up to the ultimate stress) was well addressed. The proposed model predicted well the mechanical response of the arterial tissue considering the damage of collagen fibers for both the healthy and atherosclerotic arterial walls..
11. Alireza Karimi, Razaghi Reza, Toshihiro Sera, Susumu Kudo, Mahdi Navidbakhsh, Computing the influences of different Intraocular Pressures on the human eye components using computational fluid-structure interaction model, Technology and Health Care, 25, 285-297, 2017.03.
12. Alireza Karimi, Reza Razaghi, Mahdi Navidbakhsh, Toshihiro Sera, Susumu Kudo, Mechanical properties of the human sclera under various strain rates
Elastic, hyperelastic, and viscoelastic models, Journal of Biomaterials and Tissue Engineering, 10.1166/jbt.2017.1609, 7, 8, 686-695, 2017.01, [URL], The sclera is the white outer shell of the eye which its material properties have a key asset for preserving the form of the eye against the exterior and interior musculature and fluctuations forces induce by Intraocular Pressure (IOP). Besides, the biomechanical atmosphere of the head of the optic nerve is profoundly affected by the mechanical properties of the sclera. Computational modeling is a useful way to understand various types of the injuries to the eye, such as trauma. In order to produce reliable numerical results, it is necessary to input precise material properties into the numerical models. However, so far there is a paucity of knowledge about the dissimilarity of the scleral mechanical properties/behaviors under various loading conditions. Therefore, the aim of this study was to evaluate the linear elastic and nonlinear hyperelastic mechanical properties of the human sclera at eight different strain rates. The experimental results revealed a significant role of the strain rate on the elastic modulus and maximum stress in a way that the lowest and highest elastic modulus and stress were observed at the strain rate of 5 and 200 mm/min, respectively. Due to the nonlinear mechanical behavior of the sclera, three different constitutive strain energy density functions examined and their coefficients were calculated thru the linear/nonlinear optimization method. Finally, the agreement of the constitutive data with that of the experimental ones was verified using a FE model of the tensile test. Due to the strain rate dependency of the results, the viscoelastic time-dependentmechanical behavior of the sclera was also assessed through the relaxation Prony-series formulation. Although all these three material models can be used to address the mechanical response of the sclera, according to the objective of a numerical model and its possible applications, each of which can be selected. The outcomes of the concurrent study may have implications not only for understanding the linear elastic and nonlinear hyperelastic mechanical properties of the sclera tissue under various loading rates, but also for knowing the time-dependent viscoelastic mechanical properties of the sclera under the relaxation loading..
13. Alireza Karimi, Seyed Mohammadali Rahmati, Toshihiro Sera, Susumu Kudo, Mahdi Navidbakhsh, A Combination of Constitutive Damage Model and Artificial Neural Networks to Characterize the Mechanical Properties of the Healthy and Atherosclerotic Human Coronary Arteries, Artificial Organs, 1-15, 2017.01.
14. Alireza Karimi, Toshihiro Sera, Susumu Kudo, Mahdi Navidbakhsh, Experimental verification of the healthy and atherosclerotic coronary arteries incompressibility via Digital Image Correlation, Artery Research, 16, 1-7, 2016.09.
15. Tateki Sumii, Yohei Nakano, Takuma Abe, Toshihiro Sera, Kazuhiro Nakashima, Susumu Kudo, Effects of nitric oxide on ammonia decomposition by hepatocytes under shear stress, Journal of Biorheology, in press, 2016.09.
16. Alireza Karimi, Reza Razaghi, Susumu Kudo, Mahdi Navidbakhsh, Dynamic finite element simulation of the human head under impact, loading to compare the application of PVA and EPS as helmet materials, Journal of Advanced Physics,, 5, 3, 214-219, 2016.09.
17. Alireza Karimi, Reza Razaghi, Mahdi Navidbakhsh, Toshihiro Sera, Susumu Kudo, Computing the stresses and deformations of the human eye components due to a high explosive detonation using fluid–structure interaction model, Injury, 47, 5, 1042-1050, 2016.05.
18. Alireza Karimi, Reza Razaghi, Mahdi Navidbakhsh, Toshihiro Sera, Susumu Kudo, Measurement of the mechanical properties of soccer balls using digital image correlation method, Sport Sciences for Health, 12, 1, 69-76, 2016.04.
19. Alireza Karimi, Reza Razaghi, Mahdi Navidbakhsh, Toshihiro Sera, Susumu Kudo, Dynamic finite element simulation of the gunshot injury to the human forehead protected by polyvinyl alcohol sponge, Journal of Materials Science: Materials in Medicine, 27, 74, 1-12, 2016.04.
20. Tateki Sumii, Yohei Nakano, Toshihiro Sera, Takuma Abe, Kazuhiro Nakashima, Susumu Kudo, The Effect of Nitric Oxide on Ammonia Decomposition in Co-cultures of Hepatocytes and Hepatic Stellate Cells, In Vitro Cellular & Developmental Biology - Animal, 1-7, 2016.02.
21. Alireza Karimi, Susumu Kudo, Reza Razaghi, Mahdi Navidbakhsh, Measurement of the mechanical properties of the handball, volleyball, and basketball using DIC method: a combination of experimental, constitutive, and viscoelastic models, Sport Sciences for Health, 11, 3, 295-303, 2015.12.
22. Alireza Karimi, Reza Razaghi, Mahdi Navidbakhsh, Toshihiro Sera, Susumu Kudo, Quantifying the injury of the human eye components due to tennis ball impact using a computational fluid-structure interaction model, Sports Engineering, 1-11, 2015.12.
23. Alireza Karimi, Susumu Kudo, Reza Razaghi, Mahdi Navidbakhsh, A comparative study on the mechanical properties of the healthy and varicose human saphenous vein under uniaxial loading, Journal of Medical Engineering & Technology, 10.3109/03091902.2015.1086030, 39, 8, 490-497, 2015.09.
24. Alireza Karimi, Susumu Kudo, Reza Razaghi, Mahdi Navidbakhsh, A Combination of Experimental and Numerical Analyses to Measure the Compressive Mechanical Properties of Tennis Ball, Biomed. Eng. Appl. Basis Commun., 27, 1550039 -7 pages, 2015.06.
25. Hitoshi Muguruma, Tatsuya Hoshino, Ryosuke Fujita, Takeki Sumii, Susumu Kudo, Adhesion and Alignment of Nonparenchymal Cells onto a Patterned Surface with a Two-Step Plasma Polymerization Process,, Plasma Process. Polym., 12, 8, 746-754, 2015.02.
26. Tateki Sumii, Ryosuke Fujita, Kazuo Tanishita, Susumu Kudo, Effect of flow load on hepatic function in co-culture of hepatocytes with hepatic stellate cells and endothelial cells:Relationship between hepatic function and nitric oxide concentration in vitro, Journal of Biomechanical Science and Engineering, 2012.04.
27. 工藤奨,坂口雄貴,岩永光一,隅井千城,Kamariah Binti Md Isa, 寒冷血管拡張反応時の皮膚血流応答に及ぼす環境温の影響, 日本生理人類学会誌, 17, 15-21, 2012.02.
28. Susumu Kudo, Minoru Kawarabayashi, Mariko Ikeda, and Kazuo Tanishita, Albumin Permeability across Endothelial Monolayers under Pulsatile Shear Stress, Journal of Biorheology, 10.1007/s12573-012-0045-8, 2012.02.
29. 川崎那緒人,島田知弥,福井武和,濱崎啓太,工藤奨, フォトクロミック蛍光タンパク質を用いた血管内皮細胞細胞膜タンパク質拡散の可視化解析, 日本機械学会論文集, 78, 194-205, 2012.01.
30. 工藤奨,細渕誠人,紅床省吾,隅井干城,島田知弥,寺田麻理枝,谷下一夫, せん断流れ場でのIP3誘導による内皮細間Ca2+応答伝播, 日本機械学会論文集, 77, 2431-2441, 2011.12.
31. Kamariah binti Md Isa, Naoto Kawasaki, Keiichi Ueyama, Tateki Sumii, Susumu Kudo, Effects of cold exposure and shear stress on endothelial nitric oxide synthase activation, Biochemical and Biophysical Research Communication, 412, 318-312, 2011.08.
32. 川崎那緒人,工藤 奨, フォトクロミック蛍光タンパク質を用いた血管内皮細胞内物質拡散の可視化, 日本機械学会論文集, 76-770, 217-223, 2010.10.
33. 川崎那緒人,長崎 晃,斎藤知行,野口拓哉,工藤 奨, 光転換型蛍光タンパク質を用いた血管内皮細胞の微小管構造の可視化, 日本生体医工学, 47, 6, 595-601, 2009.12.
34. Susumu KUDO, Kaoru HOSOE, Makoto HOSOBUCHI, Naoto KAWASAKI, and Kazuo TANISHITA, ATP Release from Cultured Endothelial Cells and Intercellular Calcium Signaling During Shear Stress Exposure, Journal of Biomechanical Science and Engineering, 4, 2, 274-285, 2009.06.
35. Akinori UEDA, Ryo SUDO, Mariko IKEDA, Ken-ichi KOKUBO, Susumu KUDO, Hirosuke KOBAYASHI and Kazuo TANISHITA,, Effect of Hypoxia on Formation of Three-Dimensional Microvessel Networks by Endothelial Cells in vitro, Journal of Biomechanical Science and Engineering, 3, 229-310, 2008.09.
36. Noriyuki Kataoka, Ken Hashimoto, Susumu Kudo, Ryuhei Yamaguchi, Katsuhiko Tsujioka, Fumihiko Kajiya, Intracellular Ca2+ Responses in Cultured Endothelial Cells to Mechanical Stimulation by Laser Tweezers, Journal of Biomechanical Science and Engineering, 3, 116-123, 2008.04.
37. Hitoshi Muguruma, Naoya Murata, Naoto Kawasaki, Shogo Kuretoko, and Susumu Kudo, Growth of Endothelial Cells on Surfaces Modified by a Plasma-Polymerized Coating, IEICE Trans. Electron, E90-3, 1844-1847, 2007.09.
38. Masashi Nakamura, Teruhiko Fuwa, Kaoru Inoue, Fusako Iwasaki, Susumu Kudo, Hideki Sako, Masahiko Sato, and Yoshihiro Shimomura, Prospect of Manufacturing and Design Based on Physiological Polymorphism, J. Physiological Anthropology, 24, 507-511, 2007.06.
39. 安納住子,阿部貴志,西良浩一,工藤奨,山本卓志,緒方是嗣,Vijay K.Goel, 多遺伝子座におけるSNP対立遺伝子の相互作用が及ぼすヒトの皮膚色の多様性について, 日本生理人類学会誌, 12, 1月10日, 2007.02.
40. Akinori Ueda, Ryo Sudo, Mariko Ikeda, Susumu Kudo, and Kazuo Tanishita, Initial bFGF Distribution Affects the Depth of Three-dimensinal Microvessel Networks in Vitro, Journal of Biomechanical Science and Engineering, 1, 136-146, 2006.10.
41. Susumu Kudo, Masanori Tsuzaka, Mariko Ikeda, Kazuo Tanishita, Albumin Permeability across Endothelial Monolayer’s under Long-term Shear Stress, JSME International Series C, 48, 419-424, 2005.12.
42. Sumiko Anno, Susumu Kudo, Keita Hamasaki, Kazunari Matsumura, Takushi Yamamoto and Koretsugu Ogata, Technique of SNP Genotypig and Remote Sensing Applied for Elucidation of the Contribution of Polygene and Environmental Factors to Inter-individual Variation in Skin Pigmentation Phenotype, J. Physiological Anthropology and Applied Human Science, 24, 483-486, 2005.06.
43. Susumu Kudo, Ryuhei Yamaguchi, Mariko Ikeda, Kazuo Tanishita, Effect of Fluid Force on Vascular Cell Function, J. Physiological Anthropology and Applied Human Science, 24, 459-461, 2005.06.
44. 植田晃然,古賀理基,池田満里子,工藤奨,谷下一夫, In Vitroモデルを用いた血管新生に関する研究, 慶應義塾大学日吉紀要,自然科学, 37, 1月13日, 2005.02.
45. Susumu Kudo, Kenji Ikezawa, Mariko Ikeda, and Kazuo Tanishita, Relationship between Microtubule Network Structure and Intracellular Transport in Cultured Endothelial Cells Affected by Shear Stress, JSME International Series C, 47, 977-984, 2004.12.
46. 山口隆平, 工藤奨, 山野辺博之, 中島幹雄, 杉原亮太, 氏家弘, 脈動流中の脳前交通動脈回りの壁せん断応力と動脈瘤発生, 日本機械学会論文集(B編), 66-652, 3124-3130, 2000.12.
47. 山口隆平,工藤奨,天谷英昭,重田雅也,早瀬敏幸, 直角分岐管の分岐点回りの流れ構造と枝管内流れの周期振動発生, 日本機械学会論文集(B編), 68-671, 2014-2019, 2002.07.
48. 山口隆平,工藤奨,中澤伸彦,中島幹雄,氏家弘, 動脈瘤発生に伴う脳前交通動脈付近の流れの変化, 日本機械学会論文集(B編), 68-675, 1月6日, 2002.11.
49. Susumu Kudo, Ryuhei Yamaguchi, Kazutoshi Machida, Mariko Ikeda, Kotaro Oka, and Kazuo Tanishita, Effect of Long-Term Shear Stress Exposure on Calcium Response and Morphology of Cultured Endothelial Cells, JSME International, Series C, Feb-46, 1226-1233, 2003.12.
50. Akinari Ueda, Makoto Koga, Mariko Ikeda, Susumu Kudo and Kazuo Tanishita, Effect of Shear Stress on Micro-vessel Network Formation of Endothelial Cells with in vitoro Three-dimensiona Model, Am. J. Phisiol. Heart Circ. Physiol,, 287, H994-H1002, 2004.11.
51. Takeshi Kubota, utaka Shindo, Kentaro Tokuno, Hirokazu Komatsu, Hiroto Ogawa, Susumu Kudo, Yosiichiro Kitamura, Koji Suzuki, Kotaro Oka, Mitochondria are Intracellular Magnesium Stores: Investigation by Simultaneous Fluorescent Imaging in PC12 Cells Article Type, Biochim Biophys Acta, 1744, 19-28, 2004.12.
52. Susumu Kudo, Ryoma Morigaki, Jyunichi Saito, Mariko Ikeda, Kotaro Oka, and Kazuo Tanishita, Shear-Stress Effect on Mitochondrial Membrane Potential and Albumin Uptake in Cultured Endothelial Cells, Biochemical and Biophysical Research Communication, 270, 616-621, 2000.04.
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pure2017年10月2日から、「九州大学研究者情報」を補完するデータベースとして、Elsevier社の「Pure」による研究業績の公開を開始しました。
 
 
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