Kyushu University Academic Staff Educational and Research Activities Database
List of Presentations
Hayashi Nobuya Last modified date:2019.12.31

Professor / High Density Energy Science / Department of Advanced Energy Science and Engineering / Faculty of Engineering Sciences


Presentations
1. Ryota Okabayashi, Nobuya Hayashi , Freshness Preservation of Fruit by Treatment of High Concentration Ozone, APSPT-11, 2019.12.
2. Yi-Ci Yao, Yusuke Matsunaga, Nobuya Hayashi, Enhance the Effect of LPS on Macrophage-like Cells by Atmospheric Oxygen Plasma, APSPT-11, 2019.12.
3. Sitti Subaedah Sahabuddin, Haruka Uematsu and Nobuya Hayashi, Activation and growth of EL-4 T-cell Using Atmospheric Oxygen Plasma, APSPT-11, 2019.12.
4. Yusuke Matsunaga, Yi-Ci Yao, Nobuya Hayashi, Activation of macrophage phagocytosis by atmospheric pressure plasma irradiation, Dry Process Symposium 2019, 2019.11, The purpose of this study is to examine the increase in the number of cells and the enhancement of the phagocytosis ability of macrophages when irradiated with oxygen plasma and nitrogen plasma to macrophages, which is a type of immune cells. The plasma generated by the surface discharge was irradiated to the cells through a silicon tube. The number of cells was measured using a cell counting reagent, and phagocytosis was measured using fluorescent beads. The phagocytosis ability was enhanced up to 2.5 times by oxygen plasma irradiation and up to 4 times by nitrogen plasma irradiation..
5. Sitti Subaedah, Haruka Uematsu and Nobuya Hayashi, Growth Characteristics of EL-4 T-cell with the activator CD3/CD28 Using Atmospheric Oxygen Plasma for Malaria Disease, 2019.11.
6. Nobuya Hayashi, Yi-Ci Yao and Yuki Inoue, Activation and differentiation control of osteoclast precur-sor cells irradiated with oxygen plasma, Dry Process Symposium 2019, 2019.11, Differentiation of osteoclast precursor cells is attempted to be regulated by the plasma irradiation to the cells without damages on the cells. The RANKL that is the differentiation inducer in the culture medium is modified by the plasma irradiation, and lose the function. Oxygen plasma irradiation to the cells without the culture medium at lower discharge voltage leads to the increase of the cell number. The kinase ASK1 that catalyze MAPK cascade for cell growth would be activated..
7. R. Okabayashi, N. Hayashi, Fruit surface sterilization and damage evaluation using active oxygen species by atmospheric discharge, Dry Process Symposium 2019, 2019.11, Cherry is treated by active oxygen species those are generated by surface discharge to keep its freshness for several weeks. Sterilization effect depends on the ozone treatment time when the ozone concentration is fixed at high concentration. IR spectra of fruits surface indicate that the reduction reaction is occurred in fruits surface by the plasma treatment. In case of high concentration of ozone and treatment time was 10 minute, almost all the mold of fruit surface was sterilized..
8. Y.C. Yao, N. Hayashi, The Effect on Macrophage-like Cells from Atmospheric Plasma, XXXIV ICPIG & ICRP-10, 2019.07, Plasma is used to mimic external factors to macrophage-like cells for making sure whether cell proliferation or activation. In this experiment, trace ROS is introduced to culture media and observe the variation of different treatment time. The results indicate cell got damage in the long irradiated period and lag the rate of cell cycle; in addition, cells survival is still confirmed..
9. M. Marume, Y. Miyamaru, N. Hayashi, R. Aijima, Y. Yamashita, Effect of Plasma Direct Irradiation on Oral Cancer Cells Using Torch Type Dielectric Barrier Discharge Plasma, ISPC24, 2019.06, Selective inactivation of the oral cancer cells is observed when the atmospheric oxygen plasma irradiates the oral cancer cells (HSC3) and normal cells (HaCaT) after removing culture medium. When the cells are irradiated with oxygen plasma, the intracellular active oxygen in cancer cells increases comparing with that in normal cells. Activity of caspase-3/7 in oral cancer cells, which is a kind of major biological reactions of the apoptosis, increases after oxygen plasma irradiation..
10. K. Yamamoto, N. Hayashi and K. Tashiro, Gene expression analysis of Arabidopsis thaliana irradiated with oxygen plasma, ISPC24, 2019.06, Growth enhancement are observed pure growth effects by irradiating plasma to leaves that are cells after differentiation. The growth suppression effect such as the reduction of leaf area and suppression of the sites and functions related to glycoprotein production and photosynthesis was observed. Also, the activation of chaperonin which may lead to a growth enhancement effect was observed..
11. Y. Ide, N. Hayashi, Investigation of Growth Enhancement Mechanism of Plants by Using Oxygen Plasma and Karrikin, International Conference on Plasma Medicine 7, 2018.06.
12. 林 信哉、小野大帝、米須 章、田代康介、合島怜於奈, Growth enhancement and gene expression of plants induced by oxygen plasma irradiation, International Toki Conference 2017, 2017.12, 植物種子の遺伝子発現解析とDNAメチル化定量分析を行い、プラズマ照射効果の発現機構としてのエピジェネティクスを確認した。シロイヌナズナの葉面積と全長の増加は、活性酸素種の照射線量に関係する。プラズマ照射された種子から生長した植物の生長速度は、生育期間中の未処理の種子の生育速度よりも速い。シロイヌナズナの種子の遺伝子発現は、酸素プラズマ照射のために数十の遺伝子がアップレギュレートされていることを示している。クロマチン構造およびDNAメチル化などの染色体領域の構造が改変されることが見出され、エネルギー産生系の増強を示す。ヒストンH1.2の減少およびDMEの増加は、ヒストン周辺の遺伝子巻取りおよび遺伝子の脱メチル化をそれぞれ緩やかに誘発する。したがって、遺伝子のエピジェネティックな改変により、酸素プラズマの照射効果の継承が起こることが明らかとなった。.
13. 林 信哉、三根圭介、宮丸由紀恵、合島怜於奈、山下佳雄、後藤昌昭, Regulation of activity and differentiation of animal cells using active oxygen species in atmospheric plasma, Asia-Pacific International Symposium on the Basics and Applications of Plasma Technology (APSPT-10), 2017.12, プラズマを用いた細胞不活性化技術は、がんや骨粗鬆症の治療に有効であると期待されている。 しかし、細胞へのプラズマ照射の影響とその不活性化メカニズムは依然として不明であり、特にがん細胞と正常細胞との選択性の機構解明がなされておらず,実用化の障壁となっている。 本研究では、DBDプラズマによって生成された活性酸素種の口腔がん細胞および正常細胞に対する不活性化効果を調べた。.
14. 林 信哉,中野 陸,小野大帝,田代康介,久原 哲,合島怜於奈, Modification of gene expression of plants induced by active oxygen species in oxygen plasma, IUMRS-ICAM 2017, 2017.09, 遺伝子発現解析とDNAメチル化定量解析を行い、エピジェネティクスがプラズマ照射効果の遺伝機構であることを確認した。 ヒストンH1.2の減少およびDMEの増加から、ヒストンの周りの遺伝子巻取りの緩和および遺伝子の脱メチル化が明らかとなった。 DNA脱メチル化が緩慢であることから、遺伝子発現の増加が示唆された。 また、DNAメチル化量とプラズマ中の励起酸素量が酸素ガス圧力に関して同様の傾向を有することが分かった。 以上の結果から、活性酸素種の照射により遺伝子のエピジェネティックな改変が生じ、増殖促進効果の遺伝機構がエピジェネティクスに起因すると結論づけられた。.
15. Reoto Ono, Nobuya Hayashi, Elucidation of Growth Enhancement Effect of Sprouts of Brassicaceae using Low Pressure Oxygen Plasma, Malaysia-Japan Joint International Conference 2015 (MJJIC2015), 2015.11.
16. Reoto Ono, Nobuya Hayashi, Inactivation of Bacteria on Plant Seeds by Low-Pressure RF Oxygen Plasma, Dry Process Symposium 2015, 2015.11.
17. Hiroshi Hamasaki, Nobuya Hayashi, Characteristics of Protein Decomposition by Active Species in Oxygen Plasma, Dry Process Symposium 2015, 2015.11.
18. Yasuhiro Sakai, Nobuya Hayashi, Sterilization Performance and Material Compatibility of Dental Sterilizer using RF Oxygen Plasma, Dry Process Symposium 2015, 2015.11.
19. Reoto Ono, Nobuya Hayashi, Inactivation of Pathogenic Bacteria on Seeds by Active Oxygen Species Generated in Low-Pressure Plasma, 68th Annual Gaseous Electronics Conference held jointly with 9th Annual International Conference Plasma & 33rd Symposium on Plasma Processing, 2015.10.
20. Tetsuya Ohtsubo, Nobuya Hayashi, Effect of active species on animal cells in culture media induced by DBD Plasma irradiation using air, 68th Annual Gaseous Electronics Conference held jointly with 9th Annual International Conference Plasma & 33rd Symposium on Plasma Processing, 2015.10.
21. Yasuhiro Sakai, Nobuya Hayashi, Sterilization Performance and Material Compatibility of Sterilizer for Dental Instruments using RF Oxygen Plasma, 68th Annual Gaseous Electronics Conference held jointly with 9th Annual International Conference Plasma & 33rd Symposium on Plasma Processing, 2015.10.
22. Satoshi Watanabe, Nobuya Hayashi, Mechanism of Growth Enhancement of Plants using Active Species in Air and Oxygen Plasma, 68th Annual Gaseous Electronics Conference held jointly with 9th Annual International Conference Plasma & 33rd Symposium on Plasma Processing, 2015.10.
23. Reoto Ono, Nobuya Hayashi, Inactivation of Pathogenic Bacteria by Active Oxygen Species in Low-Pressure Oxygen Plasma, 10th International Symposium on Applied Plasma Science, 2015.09.
24. Kenta Nakamura, Nobuya Hayashi, Freshness Keeping of Fruits and Inhibition of Oxidation Using Active Oxygen Species Produced by Atmospheric Pressure Discharge, 10th International Symposium on Applied Plasma Science, 2015.09.
25. Satoshi Watanabe, Nobuya Hayashi, Growth Enhancement and Change of Cells of Plants Using Various Active Species in Plasma, 10th International Symposium on Applied Plasma Science, 2015.09.
26. Reoto Ono, Nobuya Hayashi, Effect of Oxidative Stress on Cell Growth of Animal Cells Induced by DBD Plasma, 7th International Symposium on Advanced Plasma Science and Its Applications for Nitrides and Nanomaterials, 2015.03.
27. Shohei Uchida, Nobuya Hayashi, Inactivation of Pathogenic Bacterium by Oxygen Plasma Irradiation, IAPS 15, 2015.03.
28. Reoto Ono, Nobuya Hayashi, Effect of DBD Plasma on Cell Growth of Animal Cells, IAPS 15, 2015.03.
29. Akimasa Tanaka, Nobuya Hayashi, KOSUKE TASHIRO, Development of Extremely Rapid Sterilization Method Using UV Light and Elucidation of Inactivation Mechanism of Vegetative Form of Bacteria, IAPS 15, 2015.03.
30. Kenta Nakamura, Nobuya Hayashi, Sterilization of Fruit Surface and Increase of Antioxidative Activity Using Active Oxygen Species Produced by DBD, IAPS 15, 2015.03.
31. Hiroshi Hamasaki, Nobuya Hayashi, Characteristics of Protein Decomposition Using Low-pressure RF Oxygen Plasma, IAPS 15, 2015.03.
32. Nobuya Hayashi, Masaharu Shiratani, Sterilization of Agricultural Products Using Oxygen Radicals Produced by Barrier Discharge, 8th International Conference on Reactive Plasmas and 31st Symposium on Plasma Processing, 2014.02.
33. Reoto Ono, Nobuya Hayashi, Effect of antioxidative activity of plants by low pressure oxygen plasma irradiation, 8th International Conference on Reactive Plasmas and 31st Symposium on Plasma Processing, 2014.02.