| Kinichi Nakashima | Last modified dateļ¼2023.12.08 |
Professor /
Stem Cell Biology and Medicine /
Department of Stem Cell Biology and Medicine /
Faculty of Medical Sciences
Presentations
| 1. | Kinichi Nakashima, Artificial neurogenesis in the adult central nervous systems and its effects on functional recovery after injury, 2nd Neuroepigenetics & Neuroepitranscriptomics, 2020.03, [URL], Usefulness of neural stem cell (NSC) transplantation to replenish neurons has been extensively investigated. However, because of gliogenic environment in the lesion site, effective neuronal production has not been satisfactorily successful. We have previously found that antiepileptic drug valproic acid, also known as a histone deacetylase inhibitor, induced neuronal differentiation of NSCs, and reported that combinatorial treatment with VPA and NSC transplantation can supply newly generated neurons in injured spinal cord, leading to dramatic functional recovery after spinal cord injury (SCI). In the subsequent study, we have also shown that human iPSC-derived NSCs, which are epigenetically restricted not to differentiate into glial cells, efficiently give rise to neurons in the injured spinal cord, inducing recovery of locomotor functions of mice after SCI. Although it is now understood that supply of new neurons has beneficial effects on the functional recovery, there still remain critical problems, such as tumorigenicity and time-consuming preparation of cells if we utilize iPSC-derived cells. As an alternative strategy to replenish new neurons in vivo, direct conversion from endogenous cells in non-neuronal lineages has recently attracted much attention. In this regard, we have found that microglia, which converge at lesion site after injury, can be converted to functional neurons both in vitro and in vivo by the expression of a single proneural transcription factor NeuroD1. In this talk, I would like to introduce these our achievements and discuss future directions regarding treatments of CNS injuries.. |
| 2. | Kinichi Nakashima, Insights into Rett Syndrome using Neural Stem Cell, KEYSTONE SYMPOSIA, 2017.01. |
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