Kyushu University Academic Staff Educational and Research Activities Database
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Nakao Shintaro Last modified date:2019.10.02

Administration Post

Academic Degree
Field of Specialization
Research Interests
  • Investigation of Retinal/Choroidal Vascular Diseases
    keyword : Diabetic retinopathy, Diabetic macular edema, Age-related macular degeneration, Vitrectomy, Angiogenesis, Lymphangiogenesis, Inflammation, Macrophage, Robotic Surgery
Academic Activities
1. Shintaro Nakao, Yasuaki Hata, An Overview on Diabetes and Ocular Health , Elsevier, 2012.01.
1. Aliaa Barakat, Shintaro Nakao, Souska Zandi, Dawei Sun, Ruth Schmidt-Ullrich, KC Hayes, Ali Hafezi-Moghadam, In contrast to western diet, a plant-based, high-fat, low-sugar diet does not exacerbate retinal endothelial injury in streptozotocin-induced diabetes, FASEB J, 2019.06.
2. Yoshihiro Kaizu, Shintaro Nakao, Mitsuru Arima, Takehito Hayami, Iori Wada, Muneo Yamaguchi, Haruka Sekiryu, Keijiro Ishikawa, Yasuhiro Ikeda, Koh-Hei Sonoda, Flow Density in Optical Coherence Tomography Angiography is Useful for Retinopathy Diagnosis in Diabetic Patients, Scientific Reports, 2019.06.
3. Kaizu Y, Nakao S, Arima M, Wada I, Yamaguchi M, Sekiryu H, Hayami T, Ishikawa K, Ikeda Y, Sonoda KH., Capillary dropout is dominant in deep capillary plexus in early diabetic retinopathy in optical coherence tomography angiography., Acta Ophthalmol., 2019.02.
4. Kaizu Y, Nakao S, Sekiryu H, Wada I, Yamaguchi M, Hisatomi T, Ikeda Y, Kishimoto J, Sonoda KH., Retinal flow density by optical coherence tomography angiography is useful for detection of nonperfused areas in diabetic retinopathy, GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY, 10.1007/s00417-018-4122-6, 256, 12, 2275-2282, 2018.12.
5. Shintaro Nakao, Shigeo Yoshida, Yoshihiro Kaizu, Muneo Yamaguchi, Iori Wada, Tatsuro Ishibashi, Koh-hei Sonoda, Microaneurysm Detection in Diabetic Retinopathy Using OCT Angiography May Depend on Intramicroaneurysmal Turbulence, Ophthalmology Retina, 2018.11.
6. Nakao S, Zandi S, Sun D, Hafezi-Moghadam A, Cathepsin B-Mediated CD18 Shedding Regulates Leukocyte Recruitment from Angiogenic Vessels, FASEB J, 32, 143-154, 2018.01.
7. Kaizu Y, Nakao S, Wada I, Yamaguchi M, Fujiwara K, Yoshida S, Hisatomi T, Ikeda Y, Hayami T, Ishibashi T, Sonoda KH., Imaging of Retinal Vascular Layers: Adaptive Optics Scanning Laser Ophthalmoscopy Versus Optical Coherence Tomography Angiography, TRANSLATIONAL VISION SCIENCE & TECHNOLOGY, 10.1167/tvst.6.5.2, 6, 5, 2017.09.
8. Kaizu Y, Nakao S, Yoshida S, Hayami T, Arima M, Yamaguchi M, Wada I, Hisatomi T, Ikeda Y, Ishibashi T, Sonoda KH., Optical Coherence Tomography Angiography Reveals Spatial Bias of Macular Capillary Dropout in Diabetic Retinopathy, INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 10.1167/iovs.17-22306, 58, 11, 4889-4897, 2017.09.
9. Shintaro Nakao, Optical Coherence Tomography Angiography for Detecting Choroidal Neovascularization Secondary to Punctate Inner Choroidopathy, Ophthalmic Surg Lasers Imaging Retina., 10.3928/23258160-20161130-13., 47, 12, 1157-1161, 2016.12.
10. Muneo Yamaguchi, Shintaro Nakao, High-Resolution Imaging by Adaptive Optics Scanning Laser Ophthalmoscopy Reveals Two Morphologically Distinct Types of Retinal Hard Exudates, Scientific Reports, 10.1038/srep33574, 6, 2016.09.
11. Shintaro Nakao, Wide-field laser ophthalmoscopy for imaging of gas-filled eyes after macular hole surgery, Clinical Ophthalmology, 10.2147/OPTH.S109900., 10, 1623-1630, 2016.08.
12. Yoshihiro kaizu, Shintaro Nakao, Detection of airbag impact-induced cone photoreceptor damage by adaptive optics scanning laser ophthalmoscopy: a case report, BMC Ophthalmology, 10.1186/s12886-016-0275-4, 16, 2016.07.
13. Shintaro Nakao, Phenotypic transformation of intimal and adventitial lymphatics in atherosclerosis: a regulatory role for soluble VEGF receptor 2., FASEB Journal, 10.1096/fj.201500112, 30, 7, 2490-2499, 2016.07.
14. Muneo Yamaguchi, Shintaro Nakao, Vascular Normalization by ROCK Inhibitor: Therapeutic Potential of Ripasudil (K-115) Eye Drop in Retinal Angiogenesis and Hypoxia, Investigative Ophthalmology & Visual Science, 10.1167/iovs.15-17411, 57, 4, 2264-2276, 2016.04.
15. Shintaro Nakao, Yasuhiro Ikeda, Yasunori Emi, Tatsuro Ishibashi, Possibility of Müller Cell Dysfunction as the Pathogenesis of Paclitaxel Maculopathy, Ophthalmic Surg Lasers Imaging Retina., 10.3928/23258160-20151214-14., 47, 1, 81-84, 2016.01.
16. Shintaro Nakao, Yoshihiro Kaizu, Shigeo Yoshida, Tomohiro Iida, Tatsuro Ishibashi, Spontaneous remission of acute zonal occult outer retinopathy: follow-up using adaptive optics scanning laser ophthalmoscopy, Graefes Arch Clin Exp Ophthalmol, 10.1007/s00417-014-2760-x., 253, 6, 839-843, 2015.06.
17. Shintaro Nakao, ROCK-Isoform-Specific Polarization of Macrophages Associated with Age-Related Macular Degeneration, Cell Reports, 10.1016/j.celrep.2015.01.050, 10, 7, 1173-1186, 2015.02.
18. Shintaro Nakao, Molecular imaging reveals elevated VEGFR-2 expression in retinal capillaries in diabetes: A novel biomarker for early diagnosis, FASEB Journal, 2014.06.
19. Shintaro Nakao, Retinopathy in a novel model of metabolic syndrome and type 2 diabetes: new insight on the inflammatory paradigm., FASEB Journal, 10.1096/fj.12-215715., 28, 5, 2038-2046, 2014.05.
20. Shintaro Nakao, Ryoichi Arita, Wide Field Laser Ophthalmoscopy for Mice: A Novel Evaluation System for Retinal/Choroidal Angiogenesis in Mouse., Investigative Ophthalmology & Visual Science, 10.1167/iovs.13-11946., 54, 8, 5288-5293, 2013.08.
21. Shintaro Nakao, Lack of lymphatic and lymph node-mediated immunity in choroidal neovascularization, Investigative Ophthalmology & Visual Science, 10.1167/iovs.12-10341, 54, 6, 3830-3836, 2013.06.
22. Ryoichi Arita, Shintaro Nakao, A key role for ROCK in TNF-α-mediated diabetic microvascular damage., Investigative Ophthalmology & Visual Science, doi: 10.1167/iovs.12-10757, 54, 5, 2373-2383, 2013.05.
24. Toshio Hisatomi, Shintaro Nakao, The Regulatory Roles of Apoptosis-Inducing Factor in the Formation and Regression Processes of Ocular Neovascularization, American Journal of Pathology, 10.1016/j.ajpath.2012.03.022, 181, 1, 53-61, 2012.07.
25. Shintaro Nakao, Larger therapeutic window for steroid vs VEGF-A inhibitor in inflammatory angiogenesis: Surprisingly similar impact on leukocyte infiltration., Investigative Ophthalmology & Visual Science, 2012.04.
26. Shintaro Nakao, Intravitreal Anti-VEGF Therapy Blocks Inflammatory Cell Infiltration and Re-Entry into the Circulation in Retinal Angiogenesis, Investigative Ophthalmology & Visual Science, 10.1167/iovs.11-9119, 53, 7, 4323-4328, 2012.06.
27. Shintaro Nakao, Lymphangiogenesis and angiogenesis: concurrence and/or dependence? Studies in inbred mouse strains, FASEB Journal, 10.1096/fj.09-134056, 24, 2, 504-513, 2010.02.
28. Shintaro Nakao, Dexamethasone inhibits interleukin-1 beta-induced corneal neovascularization - Role of nuclear factor-kappa B-activated stromal cells in inflammatory angiogenesis, American Journal of Pathology, 10.2353/ajpath.2007.070172, 171, 3, 1058-1065, 2007.09.
29. Shintaro Nakao, Infiltration of COX-2-expressing macrophages is a prerequisite for IL-1beta-induced neovascularization and tumor growth., Journal of Clinical Investigation, 2005.11.
30. Shintaro Nakao, Synergistic effect of TNF-alpha in soluble VCAM-1-induced angiogenesis through alpha 4 integrins, Journal of Immunology, 170, 11, 5704-5711, 2003.06.
1. Nakao S, Yoshida S, Kaizu Y, Wada I, Sonoda KH, Detection Capability of OCT Angiography for Nonperfused Areas in Diabetic Retinopathy, 10th Joint Meeting of Korea-China-Japan Ophthalmologists, 2017.11.
2. 中尾 新太郎, Evaluation of hyperreflective foci in diabetic macular edema by ultra-high resolution en face optical coherence tomography, ARVO, 2017.05.
3. 中尾 新太郎, Impact of Anti-VEGF Therapy on Photoreceptor in Patients with Exudative Age-Related Macular Degeneration, 1st Japan-Taiwan Vitreoretinal Joint Meeting, 2016.10.
4. 中尾 新太郎, Impact of Anti-VEGF Therapy on Photoreceptor in Patients with Exudative Age-Related Macular Degeneration, 49th Annual Retina Society Meeting, 2016.09.
5. Shintaro Nakao, Evaluation of Vascular Lesion Before and After Anti-VEGF Therapy for Age-Related Macular Degeneration by OCT Angiography, The 54th Annual Meeting of Japanese Retina and Vitreous Society, 2015.12.
6. Shintaro Nakao, ROCK as a therapeutic target in diabetic retinopathy. Molecular Mechanisms in Diabetic Retinopathy, Asia ARVO 2015, 2015.02.
7. Shintaro Nakao, Rhoichi Arita, Muneo Yamaguchi, Tatsuro Ishibashi, Characterization of Microaneurysm in Diabetic Retinopathy Using Adaptive Optics Scanning Laser Ophthalmoscopy, ARVO.
8. Shintaro Nakao, Rhoichi Arita, Tomoyuki Isobe, Yoshio Kaneko, Tatsuro Ishibashi, Therapeutic potential of topical ROCK inhibitor K-115 in retinal neovascularization, World Ophthalmology Congress 2014, 2014.04.
9. Shintaro Nakao, Souska Zandi, Riichiro Kohno, Dawei Sun, Lack of Lymphatics and Lymph Node-mediated Immunity in Choroidal Neovascularization , The Sixth Joint Meeting of Chinese-Korean-Japanese Ophthalmologists, 2013.09, Purpose. Inflammation and immune cells regulate choroidal neovascularization (CNV) and could become therapeutic targets in age-related macular degeneration (AMD). Lymphangiogenesis is a key component of various inflammatory diseases. Whether lymphangiogenesis and lymph node-mediated immunity are involved in the pathogenesis of AMD is not understood.
Methods. To investigate lymphangiogenesis in AMD, we generated choroidal neovascularization (CNV) in animals by laser and studied surgically removed CNV membranes from uveitis and AMD patients. Immunohistochemistry was performed with LYVE-1 and podoplanin antibodies. VEGF-C and VEGFR-3 expressions were examined with immunohistochemistry and western blotting. To examine the role of lymph node in CNV, we lasered lymphotoxin alpha deficient mice (LTα-/-) and measured the CNV volume.
Results. Immunohistochemistry showed that LYVE-1(+) macrophages infiltrated in acutely induced CNV, however, lymphatic tubes did not form. CNV membranes from patients did not show LYVE-1(+)podoplanin(+) vessels, suggesting lack of lymphangiogenesis in AMD and uveitis. Western blots and immunostaining revealed VEGF-C and VEGFR-3 expression in CNV lesions, mainly in macrophages and angiogenic endothelial cells. Using fluorescent microsphere tracers, we show a path for cellular migration from the eye to the cervical lymph nodes (LN) during CNV. However, CNV injury did not cause LN swelling. CNV volume did not differ between WT and LN deficient mice, suggesting that LN is not a key component of early CNV formation.
Conclusion. Laser-induced CNV is not primarily dependent on acquired immunity, nor does the fundus injury affect peripheral LNs. Our results reveal a previously unknown cellular connection between the ocular fundus and the cervical LNs. This connection that in function resembles lymphatics is actively utilized in CNV.
10. 中尾 新太郎, Altred Vascular Microenvironment by anti-VEGF therapy in Diabetic Fibrovascular Membrane, EuroLam Retina, 2013.05.
Membership in Academic Society
  • The Retina Society
  • Japanese Ophthalmological Society
  • Japan Ophthalmologist Association
  • The Japan Diabetes Society
  • The Japanse Society of Ophthalmic Diabetology
  • Japanese Society for Ocular Circulation
  • Japanese Ocular Inflammation Society
  • Japanese Retina and Vitreous Society
  • The Japanese Vascular Biology and Medicine Organization
  • The Association for Research in Vision and Ophthalmology
  • Impact of Anti-VEGF Therapy on The Cellular Microenvironment in Retinal Angiogenesis
Educational Activities