Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Yuta Kohro Last modified date:2023.12.07

Associate Professor / Department of Life Innovation / Department of Pharmaceutical Health Care and Sciences / Faculty of Pharmaceutical Sciences


Papers
1. Teruaki Ono, Tomohiro Yamashita, Ryota Kano, Mariko Inoue, Shota Okada, Koki Kano, Schuichi Koizumi, Kazuhisa Iwabuchi, Yoshio Hirabayashi, Ichiro Matsuo, Yasuharu Nakashima, Hiroyuki Kamiguchi, Yuta Kohro, Makoto Tsuda, GPR55 contributes to neutrophil recruitment and mechanical pain induction after spinal cord compression in mice., Brain, behavior, and immunity, 10.1016/j.bbi.2023.03.008, 110, 276-287, 2023.05.
2. Heather Leduc-Pessah, Cynthia Xu, Churmy Y Fan, Rebecca Dalgarno, Yuta Kohro, Sydney Sparanese, Nikita N Burke, Kenneth A Jacobson, Christophe Altier, Daniela Salvemini, Tuan Trang, Spinal A 3 adenosine receptor activation acutely restores morphine antinociception in opioid tolerant male rats, Journal of neuroscience research, 10.1002/jnr.24869, 2022.01.
3. Charlie H T Kwok, Yuta Kohro, Michael Mousseau, Melissa S O'Brien, John R Matyas, Jason J McDougall, Tuan Trang, Role of Primary Afferents in Arthritis Induced Spinal Microglial Reactivity, Frontiers in immunology, 10.3389/fimmu.2021.626884, 2021.04.
4. Yuta Kohro, Tsuyoshi Matsuda, Kohei Yoshihara, Keita Kohno, Keisuke Koga, Ryuichi Katsuragi, Takaaki Oka, Ryoichi Tashima, Sho Muneta, Takuya Yamane, Shota Okada, Kazuya Momokino, Aogu Furusho, Kenji Hamase, Takumi Oti, Hirotaka Sakamoto, Kenichiro Hayashida, Ryosuke Kobayashi, Takuro Horii, Izuho Hatada, Hidetoshi Tozaki-Saitoh, Katsuhiko Mikoshiba, Verdon Taylor, Kazuhide Inoue, Makoto Tsuda, Spinal astrocytes in superficial laminae gate brainstem descending control of mechanosensory hypersensitivity., Nature neuroscience, 10.1038/s41593-020-00713-4, 23, 11, 1376-1387, 2020.11.
5. Teruaki Ono, Yuta Kohro, Keita Kohno, Hidetoshi Tozaki-Saitoh, Yasuharu Nakashima, Makoto Tsuda, Mechanical pain of the lower extremity after compression of the upper spinal cord involves signal transducer and activator of transcription 3-dependent reactive astrocytes and interleukin-6., Brain, behavior, and immunity, 10.1016/j.bbi.2020.07.025, 2020.07.
6. Keisuke Koga, Ryo Yamagata, Keita Kohno, Takuya Yamane, Miho Shiratori-Hayashi, Yuta Kohro, Hidetoshi Tozaki-Saitoh, MakotoTsuda, Sensitization of spinal itch transmission neurons in a mouse model of chronic itch requires an astrocytic factor., Journal of Allergy and Clinical Immunology, doi: 10.1016/j.jaci.2019.09.034, 2019.11.
7. Keita Kohno, Junko Kitano, Yuta Koro, Hidetoshi Saitoh, Kazuhide Inoue, Tsuda Makoto, Temporal kinetics of microgliosis in the spinal dorsal horn after peripheral nerve injury in rodents, Biological and Pharmaceutical Bulletin, 10.1248/bpb.b18-00278, 41, 7, 1096-1102, 2018.01, Neuropathic pain, a highly debilitating chronic pain following nerve damage, is a reflection of the aberrant functioning of a pathologically altered nervous system. Previous studies have implicated activated microglia in the spinal dorsal horn (SDH) as key cellular intermediaries in neuropathic pain. Microgliosis is among the dramatic cellular alterations that occur in the SDH in models of neuropathic pain established by peripheral nerve injury (PNI), but detailed characterization of SDH microgliosis has yet to be realized. In the present study, we performed a short-pulse labeling of proliferating cells with ethynyldeoxyuridine (EdU), a marker of the cell cycle S-phase, and found that EdU+ microglia in the SDH were rarely observed 32h after PNI, but rapidly increased to the peak level at 40h post-PNI. Numerous EdU+ microglia persisted for the next 20h (60h post-PNI) and decreased to the baseline on day 7. These results demonstrate a narrow time window for rapidly inducing a proliferation burst of SDH microglia after PNI, and these temporally restricted kinetics of microglial proliferation may help identify the molecule that causes microglial activation in the SDH, which is crucial for understanding and managing neuropathic pain..
8. Ryoichi Tashima, Keisuke Koga, Misuzu Sekine, Kensho Kanehisa, Yuta Koro, Keiko Tominaga, Katsuyuki Matsushita, Hidetoshi Saitoh, Yugo Fukazawa, Kazuhide Inoue, Hiromu Yawo, Hidemasa Furue, Tsuda Makoto, Optogenetic activation of non-nociceptive aβ fibers induces neuropathic pain-like sensory and emotional behaviors after nerve injury in rats, eNeuro, 10.1523/ENEURO.0450-17.2018, 5, 1, 2018.01, Neuropathic pain is caused by peripheral nerve injury (PNI). One hallmark symptom is allodynia (pain caused by normally innocuous stimuli), but its mechanistic underpinning remains elusive. Notably, whether selective stimulation of non-nociceptive primary afferent Aβ fibers indeed evokes neuropathic pain-like sensory and emotional behaviors after PNI is unknown, because of the lack of tools to manipulate Aβ fiber function in awake, freely moving animals. In this study, we used a transgenic rat line that enables stimulation of non-nociceptive Aβ fibers by a light-activated channel (channelrhodopsin-2; ChR2). We found that illuminating light to the plantar skin of these rats with PNI elicited pain-like withdrawal behaviors that were resistant to morphine. Light illumination to the skin of PNI rats increased the number of spinal dorsal horn (SDH) Lamina I neurons positive to activity markers (c-Fos and phosphorylated extracellular signal-regulated protein kinase; pERK). Whole-cell recording revealed that optogenetic Aβ fiber stimulation after PNI caused excitation of Lamina I neurons, which were normally silent by this stimulation. Moreover, illuminating the hindpaw of PNI rats resulted in activation of central amygdaloid neurons and produced an aversion to illumination. Thus, these findings provide the first evidence that optogenetic activation of primary afferent Aβ fibers in PNI rats produces excitation of Lamina I neurons and neuropathic pain-like behaviors that were resistant to morphine treatment. This approach may provide a new path for investigating circuits and behaviors of Aβ fiber-mediated neuropathic allodynia with sensory and emotional aspects after PNI and for discovering novel drugs to treat neuropathic pain..
9. Kohei Yoshihara, Tsuyoshi Matsuda, Yuta Koro, Hidetoshi Saitoh, Kazuhide Inoue, Tsuda Makoto, Astrocytic Ca2+ responses in the spinal dorsal horn by noxious stimuli to the skin, Journal of Pharmacological Sciences, 10.1016/j.jphs.2018.04.007, 2018.01, The role of astrocytes in the spinal dorsal horn (SDH) for sensory information processing under normal conditions is poorly understood. In this study, we investigated whether SDH astrocytes respond to noxious and innocuous stimuli to the skin of normal mice using in vivo two-photon Ca2+ imaging under anesthesia. We found that noxious stimulation evoked by intraplantar formalin injection provoked an elevation in intracellular Ca2+ levels in SDH astrocytes. By contrast, neither instantaneous noxious pinching nor innocuous stimuli (cooling or brushing) to the hindpaw elicited astrocytic Ca2+ responses. Thus, SDH astrocytes could respond preferentially to a strong and/or sustained noxious stimulus..
10. Keisuke Koga, Kensho Kanehisa, Yuta Kohro, Miho Shiratori-Hayashi, Hidetoshi Saitoh, Kazuhide Inoue, Hidemasa Furue, Makoto Tsuda, Chemogenetic silencing of GABAergic dorsal horn interneurons induces morphine-resistant spontaneous nocifensive behaviours, SCIENTIFIC REPORTS, 10.1038/s41598-017-04972-3, 7, 2017.07.
11. Kensho Kanehisa, Miho Shiratori-Hayashi, Keisuke Koga, Hidetoshi Saitoh, Yuta Kohro, Kenji Takamori, Makoto Tsuda, Specific activation of inhibitory interneurons in the spinal dorsal horn suppresses repetitive scratching in mouse models of chronic itch, Journal of Dermatological Science, https://doi.org/10.1016/j.jdermsci.2017.05.017, 2017.06.
12. Takahiro Masuda, Yuta Kohro, Kazuhide Inoue, Makoto Tsuda, Peripheral Nerve Injury: a Mouse Model of Neuropathic Pain, Bio-protocol, https://doi.org/10.21769/BioProtoc.2252, 2017.05.
13. Satoru Koyanagi, Naoki Kusunose, Marie Taniguchi, Akamine Takahiro, Yuki Kanado, Yui Ozono, Takahiro Masuda, Yuta Kohro, Naoya Matsunaga, Makoto Tsuda, Michael W salter, Kazuhide Inoue, Shigehiro Ohdo, Glucocorticoid regulation of ATP release from spinal astrocytes underlies diurnal exacerbation of neuropathic mechanical allodynia, NATURE COMMUNICATIONS, 10.1038/ncomms13102, 7, 2016.10.
14. Takahiro Masuda, Yui Ozono, Satsuki Mikuriya, Yuta Kohro, Hidetoshi Tozaki-Saitoh, Ken Iwatsuki, Hisayuki Uneyama, Reiko Ichikawa, Michael W salter, Makoto Tsuda, Kasuhide Inoue, Dorsal horn neurons release extracellular ATP in a VNUT-dependent manner that underlies neuropathic pain, NATURE COMMUNICATIONS, 10.1038/ncomms12529, 7, 2016.08.
15. Ryoichi Tashima, Daisuke Tomiyama, Miho Shiratori, Tomohiro Yamashita, Yuta Kohro, Hidetoshi Saitoh, Kazuhide Inoue, Makoto Tsuda, Bone marrow-derived cells in the population of spinal microglia after peripheral nerve injury, SCIENTIFIC REPORTS, 10.1038/srep23701, 6, 2016.03.
16. Yuta Kohro, Emi Sakaguchi, Ryoichi Tashima, Hidetoshi Saitoh, Hideyuki Okano, Kazuhide Inoue, Takahiro Masuda et al., A new minimally-invasive method for microinjection into the mouse spinal dorsal horn., Scientific Reports, doi: 10.1038/srep14306., 2015.09.
17. Miho Shiratori, Keisuke Koga, Hidetoshi Saitoh, Yuta Kohro, Honami Toyonaga, Chiharu Yamaguchi, Ayumi Hasegawa, Takeshi Nakahara, Junichi Hachisuka, Shizuo Akira, Hideyuki Okano, Masutaka Furue, Kazuhide Inoue, Makoto Tsuda, STAT3-dependent reactive astrogliosis in the spinal dorsal horn underlies chronic itch, NATURE MEDICINE, 10.1038/nm.3912, 21, 8, 927-931, 2015.08.
18. Tsuda M, Kohro Y, Yano T, Tsujikawa T, Kitano J, Tozaki-Saitoh H, Koyanagi S, Ohdo S, Ji RR, Salter MW, Inoue K, JAK-STAT3 pathway regulates spinal astrocyte proliferation and neuropathic pain maintenance in rats., BRAIN, 134, 4, 1127-1139, 2011.04.
19. Shigeo Hasegawa, Yuta Kohro, Miho Shiratori, Satoshi Ishii, Takao Shimizu, Makoto Tsuda, Kazuhide Inoue, Role of PAF Receptor in Proinflammatory Cytokine Expression in the Dorsal Root Ganglion and Tactile Allodynia in a Rodent Model of Neuropathic Pain, PLOS ONE, 10.1371/journal.pone.0010467, 5, 5, 2010.05.
20. Shigeo Hasegawa, Yuta Kohro, Makoto Tsuda, Kazuhide Inoue, Activation of cytosolic phospholipase A2 in dorsal root ganglion neurons by Ca2+/calmodulin-dependent protein kinase II after peripheral nerve injury., Mol Pain, 2009.05.