Kyushu University Academic Staff Educational and Research Activities Database
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Seiichi Sakamoto Last modified date:2019.11.07

Assistant Professor / Department of Pharmaceutical Health Care and Sciences
Department of Pharmaceutical Health Care and Sciences
Faculty of Pharmaceutical Sciences


Graduate School
Undergraduate School
Other Organization


E-Mail
Homepage
http://seigyo.phar.kyushu-u.ac.jp.
Academic Degree
Ph. D.
Field of Specialization
Pharmacognosy
Total Priod of education and research career in the foreign country
00years03months
Research
Research Interests
  • Development of high-throughput analytical methods using antibody against plant secondary metabolites
    keyword : Monoclonal antibody、scFv、Plant secondary metabolites、High-throughput analytical methods、fluorescence sensor
    2013.10.
  • Functional analysis of biosynthesis enzyme of plant secondary metabolites
    keyword : Metabolic engineering、Plant secondary metabolites、Biosynthesis
    2013.10.
  • Elucidation of the plant cell death and their regulation mechanisms
    keyword : Plant cell death、allelopathy
    2013.10.
  • Molecular breeding of medicinal plant toward the enhancement of biosynthesis of useful secondary metabolites
    keyword : Plant cell cuulture, molecular breeding, Glycyrrhiza uralensis, Glycyrrhiza glabra, Panax Ginseng
    2018.10.
Academic Activities
Reports
1. Sakamoto S., Putalun W., Vimolmangkang S., Phoolcharoen W., Shoyama Y., Tanaka H., Morimoto S., Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites, Journal of Natural Medicines, 72, 1, 32-42, 2018, 2018.01.
2. Sakamoto S., Tanaka H., Morimoto S., Towards the prophylactic and therapeutic use of human neutralizing monoclonal antibodies for Middle East respiratory syndrome coronavirus (MERS-CoV), Annals of Translational Medicine, 3(3), 35, 2015.03.
Papers
1. Sakamoto S., Wada S., Morita Y., Yamaguchi T., Tanaka H., Morimoto S., Magnetic particles-based enzyme immunoassay for rapid determination of secoiridoid glycoside, amarogentin, Talanta, 194, 731-736, 2019.03.
2. Sakamoto S., Wada S., Tanaka H., Morimoto S., Sensitive quantitative analysis of the bitter glycoside, amarogentin by specific monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay, RSC Advances, 8, 31, 17410-17416, 2018.05.
3. Sakamoto S., Miyamoto T., Usui K, Tanaka H., Morimoto S., Sodium-periodate mediated harringtonine derivatives and their antiproliferative activity against HL-60 acute leukemia cells, Journal of Natural Products, 81, 1, 34-40, 2018.01.
4. Sakamoto S., Yusakul G., Tsuneura Y., Putalun W., Usui K., Miyamoto T., Tanaka H., Morimoto S., Sodium periodate-mediated conjugation of harringtonine enabling the production of a highly specific monoclonal antibody, and the development of a sensitive quantitative analysis method, Analyst, 142, 7, 1140-1148, 2017.04.
5. Sakamoto S., Nagamitsu R., Yusakul G., Miyamoto T., Tanaka H., Morimoto S., Ultrasensitive immunoassay for monocrotaline using monoclonal antibody produced by N, N’-carbonyldiimidazole mediated hapten-carrier protein conjugates, Talanta, 168, 67-72, 2017.05.
6. Sakamoto S., Yusakul G., Nuntawong P., Kitisripanya T., Putalun W., Miyamoto T., Tanaka H., Morimoto S., Development of an indirect competitive immunochromatographic strip test for rapid detection and determination of anticancer drug, harringtonine, Journal of Chromatography B, 1048, 150-154, 2017.03.
7. Sakamoto S., Yusakul G., Pongkitwitoon B., Tanaka H., Morimoto S., Colloidal gold-based indirect competitive immunochromatographic assay for rapid detection of bioactive isoflavone glycosides daidzin and genistin in soy products, Food Chemistry, doi.org/10.1016/j.foodchem.2015.08.002, 194, 191-195, 2016.03.
8. Sakamoto S., Uto T., Shoyama Y., Effect of glycyrrhetinic acid on lipid raft model at the air/water interface, Biochimica et Biophysica Acta, doi: 10.1016/j.bbamem.2014.11.014, 1848, 2, 434-443, 2015.02.
Presentations
1. Sakamoto S., Immunoassays for cephalotaxus alkaloids, harringtonine and antiproliferative activity of its derivatives, 5th International Conference on Pharmaceuticals, Nutraceuticals and Cosmetic Science (IPNaCS 2017), 2017.08.
2. Sakamoto S., Sodium periodate-mediated conjugation of harringtonine enabling the production of a highly specific monoclonal antibody, and the development of a sensitive quantitative analysis method, International Annual Meeting in Pharmaceutical Sciences (JSPS-NRCT 2017 and IAMPS 33), 2017.03.
3. Komatsu S., Sakamoto S., Yusakul G., Putalun W., Miyamoto T., Tanaka H., Morimoto S., A single-chain variable fragment antibody against anti-leukemia agent, harringtonine as a tool for immunomodulation, The 9th Joint Natural Products Conference 2016 in Copenhagen, 2016.07.
4. Sakamoto S., Yusakul G., Nagamitsu R., Tsuneura Y., Putalun W., Miyamoto T., Tanaka H., Morimoto S., Development of highly sensitive immunological techniques for determination of cephalotaxus alkaloids, harringtonine, The 9th Joint Natural Products Conference 2016 in Copenhagen, 2016.07.
5. Sakamoto S., Yusakul G., Nagamitsu R., Tsuneura Y., Miyamoto T., Tanaka H., Morimoto S., Production of highly specific monoclonal antibody against representative cephalotaxine alkaloids, harringtonine and its application to sensitive quantitative/qualitative analyses, The 6th International Conference on Natural Products for Health and Beauty (NATPRO 6), 2016.01.
6. Sakamoto S., Putalun W., Pongkitwitoon B., Shoyama Y., Tanaka H., Morimoto S., Modulation of plumbagin production in Plumbago zeylanica using a single-chain variable fragment antibody against plumbagin, The 1st International Conference on Herbal and Traditional Medicine (HTM 2015), 2015.01.
7. Sakamoto S., Nakahara H., Shibata O., Miscibility behavior of sphingomyelin with phytosterol derivatives by a Langmuir monolayer approach, 27th Conference of the European Colloid and Interface Society (27th ECIS), 2013.09.
8. Sakamoto S., Nakahara H., Shibata O., Specific effect of glycyrrhetinic acid on lipid raft model membrane: A Langmuir monolayer study, 27th Conference of the European Colloid and Interface Society (27th ECIS), 2013.09.
9. Sakamoto S., Nakahara H., Shibata O., Interaction of glycyrrhizin with lipid rafts model by Langmuir monolayer study, 26th Conference of the European Colloid and Interface Society (26th ECIS), 2012.09.
10. Sakamoto S., Shibata O., Nakahara H., Interaction of glycyrrhizin with the ternary mixture of sphingomyelin/DOPC/cholesterol by Langmuir monolayer study, The 4th Asian Conference on Colloid and Interface Science, 2011.11.
11. Sakamoto S., Pongkitwitoon B., Nakamura S., Maenaka K., Tanaka H., Morimoto S., Efficient silkworm expression of single-chain variable fragment antibody against ginsenoside Re using Bombyx mori nucleopolyhedrovirus bacmid DNA system and its application in ELISA for quality control of total ginsenosides, 58th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research, 2010.08.
12. Sakamoto S., Taura F., Putalun W., Pongkitwitoon B., Tsuchihashi R., Kinjo J., Tanaka H., Morimoto S., Development of fluorescence-linked immunosorbent assay using a fusion protein of green fluorescent protein and single chain variable fragment antibody against bioactive naphthoquinone, plumbagin, Asian Symposium for Pharmaceutical Science in JSPS Asian Core Program, 2009.11.
13. Sakamoto S., Taura F., Tsuchihashi R., Putalun W., Kinjo J., Tanaka H., Morimoto S., Expression, purification, and characterization of anti plumbagin single-chain variable fragment antibody in Sf9 insect cells, Asian Federation for Pharmaceutical Sciences 2009, 2009.10.
14. Sakamoto S., Sakoda J., Morinaga O., Putalun W., Tsuchihashi R., Morimoto S., Kinjo J., Tanaka H., Determination of vitamin K3 by an indirect competitive ELISA using monoclonal antibodies against plumbagin, NRCT JSPS Core University Program on Natural Medicine in Pharmaceutical Sciences, The 8th Joint Seminar (INNOVATIVE RESEARCH IN NATURAL PRODUCTS FOR SUSTAINABLE DEVELOPMENT), 2009.02.
15. Sakamoto S., Taura F., Putalun W., Tsuchihashi R., Morimoto S., Kinjo J., Tanaka H., Construction and expression of specificity-improved single-chain variable fragments against the bioactive naphthoquinone, plumbagin and its application in an enzyme-linked immunosorbent assay, The 4th KSP-JSP-CCTCNM Joint Symposium on Pharmacognosy (KOREA-JAPAN-CHINA Joint Symposium 2008), 2008.06.
Membership in Academic Society
  • The Japanese Society of Pharmacognosy
  • The Pharmaceutical Society of Japan