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Nana Shirakigawa Last modified date:2018.06.08

Assistant Professor / Molecular and Biochemical Systems Engineering
Department of Chemical Engineering
Faculty of Engineering

Academic Degree
Doctor of Engineering
Field of Specialization
Tissue engineering, Whole organ engineerin
Outline Activities
Our aim is the development of whole organ engineering based on the decellularized organ. We study about liver, blood vessel and bile duct construction. We also study about the development of biomaterial for construction of suitable cell environment to construct organ.
Research Interests
  • Functional hydrogel tube for artificial bile duct
    keyword : artificial bile duct, tissue engineering
  • Whole liver engineering using decellularized liver
    keyword : Tissue engineering, liver
Academic Activities
1. Shirakigawa N., Ijima H., Decellularization of Liver and Organogenesis in Rats, Humana Press, 2017.08, Recently, organ construction has been attempted using decellularized organs. In this study, we used decellularized rat liver to construct liver tissue by recellularization. The right lobe of the rat liver was decellularized with 4% Triton X-100 solution, recellularized with 10^7 rat hepatocytes, and albumin synthesis in the recellularized right lobe was observed. Therefore, we introduce a method of decellularizing rat liver, which retains its fine vascular structure after removal of all the cells, perform organogenesis using the decellularized liver, and evaluate the structural and functional properties of the products..
2. Nana Shirakigawa, Hiroyuki Ijima, Decellularized tissue engineering, Springer Verlag,, 185-226, 2017.01, Tissue Engineering consists of cells, a scaffold and cytokines. Decellularization represents the removal of cells from tissues or organs. Recently, decellularized tissue has been investigated as a scaffold for tissue engineering, termed decellularized tissue engineering. Importantly, the decellularized organ retains its original structure, which is then used as a template for organ construction. The decellularized organ also retains the tissue-specific extracellular matrix. Therefore, decellularized tissue can be used as a matrix to provide a suitable microenvironment for inoculated cells. Based on these concepts, the reconstruction of tissues/organs with decellularized tissue/organ has been attempted using decellularized tissue engineering. In this chapter, we introduce the typical methods used, history and attainment level for the reconstruction of specific tissues/organs. First, the different decellularized techniques and characteristics are introduced. Then, the commonly used analysis methods and cautionary points during decellularization and reconstruction with decellularized tissues/organs are explained. Next, the specific methods and characteristics of decellularized tissue engineering for specific tissues/organs are introduced. In these sections, the current conditions, problems and future work are explained. Finally, we conclude with a summary of this chapter..
1. Nana Shirakigawa, Tadamitsu IDEGUCHI, Kazuyuki ICHIKAWA, Takahisa IZUMI, Michiko HIGASHI, Shizunari YAMAMOTO, Hiroyuki Ijima, Quantitative Analysis of Vascular Structure in Decellularized Liver Using 3D Computed Tomography, Advanced Biomedical Engineering, 10.14326/abe.4.179, 4, 179-185, 2015.08.
2. Nana Shirakigawa, Takayuki Takei, Hiroyuki Ijima, Base structure consisting of an endothelialized vascular-tree network and hepatocytes for whole liver engineering, Journal of Bioscience and Bioengineering,, 116, 6, 740-745, 2013.12, Reconstructed liver has been desired as a liver substitute for transplantation. However, reconstruction of a whole liver has not been achieved because construction of a vascular network at an organ scale is very difficult. We focused on decellularized liver (DC-liver) as an artificial scaffold for the construction of a hierarchical vascular network. In this study, we obtained DC-liver and the tubular network structure in which both portal vein and hepatic vein systems remained intact. Furthermore, endothelialization of the tubular structure in DC-liver was achieved, which prevented blood leakage from the tubular structure. In addition, hepatocytes suspended in a collagen sol were injected from the surroundings using a syringe as a suitable procedure for liver cell inoculation. In summary, we developed a base structure consisting of an endothelialized vascular-tree network and hepatocytes for whole liver engineering..
3. Nana Shirakigawa, Hiroyuki Ijima, Nucleus number in clusters of transplanted fetal liver cells increases by partial hepatectomy of recipient rats, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 10.1016/j.jbiosc.2012.11.015, 115, 5, 568-570, 2013.05.
4. Nana Shirakigawa, Hiroyuki Ijima, Takayuki Takei, Decellularized liver as a practical scaffold with a vascular network template for liver tissue engineering, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 10.1016/j.jbiosc.2012.05.022, 114, 5, 546-551, 2012.11.
1. Nana Shirakigawa, Hiroyuki Ijima, Albumin production of re-cellularized liver substitute based on whole organ engineering, 2013 Joint of Japan/Taiwan/Korea Chemical Engineering Conference, 2013.11.
  • Base structure consisting of an endothelialized vascular-tree network and hepatocytes for whole liver engineering