| 1. |
國崎 祐哉, 骨髄微小環境のイメージング, 第２９回日本フローサイトメトリー学会学術集会, 2019.05. |
| 2. |
國崎 祐哉, 造血幹細胞ニッチと造血器腫瘍, 第73回日本癌学会学術総会, 2014.09. |
| 3. |
國崎 祐哉, 血管性ニッチによる造血幹細胞制御機構, 第87回日本生化学会大会, 2014.10. |
| 4. |
Yuya Kunisaki, The distinct vascular niches determine hematopoietic stem cell fate, 第76回日本 血液学会学術集会, 2014.10. |
| 5. |
Yuya Kunisaki, Arteriolar niches for hematopoietic stem cells, 第24回Hot Spring Harbor Symposium, 2014.11. |
| 6. |
Yuya Kunisaki, NG2+ arteriolar pericytes form niches for dormant hematopoietic stem cells, US-Japan Meeting on Malignant Hematopoisis and Stem Cells, 2015.03. |
| 7. |
國崎 祐哉, 骨髄微小環境による造血幹細胞制御機構, 第39回阿蘇シンポジウム, 2015.07. |
| 8. |
國崎 祐哉, 血管性ニッチによる造血幹細胞制御機構, 第33回日本骨代謝学会学術総会, 2015.07. |
| 9. |
Yuya Kunisaki, Imaging of the hematopoietic stem cells and niches, The International Society for EXPERIMENTAL HEMATOLOGY 44th Annual meeting, 2015.09. |
| 10. |
國崎 祐哉, 造血幹細胞ニッチ, 血液学会学術集会, 2015.10. |
| 11. |
國崎 祐哉, 間葉系幹細胞による造血幹細胞制御機構, 第60回日本リウマチ学会総会・学術集会, 2016.04. |
| 12. |
國崎 祐哉, 骨髄微小環境のイメージング, 第31回日本整形外科学会基礎学術集会, 2016.10. |
| 13. |
國崎 祐哉, 間葉系幹細胞による造血幹細胞制御機構, 第122回日本解剖学会総会・全国学術集会, 2017.03. |
| 14. |
Yuya Kunisaki, NG2+ arteriolar parasites form niches for dormant hematopoietic stem cells, The 12th Stem Cell Research Symposium, 2014.05, Cell cycle quiescence is a critical feature contributing to hematopoietic stem cell (HSC) maintenance. Whether quiescence and proliferation of HSCs are regulated by spatially distinct niches is unclear. Whole-mount confocal immunofluorescence assessment revealed an evenly distributed sinusoidal network and rare small calibre arterioles specifically ensheathed by NG2+ perivascular cells, which were found predominantly in close proximity to the bone. We further found that ~40 % of HSCs were located within 20µm distance from arterioles. To assess the significance of this association, we developed a computational simulation, in which HSCs were randomly positioned on images of whole-mount prepared sterna. The observed mean distance to arterioles was statistically different from that of randomly placed HSCs. This suggests that the physical association of HSC with arterioles is significant. Prolonged quiescence is referred to as dormancy and tracked as label-retaining cells. We evaluated the spatial association of dormant HSCs with arterioles by pulse-chase experiments with EdU and found that significantly more EdU+ HSCs compared to total HSCs were located within 20µm distance from arterioles. To test the functional relevance, we evaluated HSC-arteriole associations in mice treated with 5FU. We found that on day 3 the vast majority of HSCs was closely associated with arterioles. These data together indicate that arterioles provide a milieu promoting quiescence for both HSCs and a safe harbor to protect them against genotoxic insults. To examine the function of NG2+ cells, we intercrossed NG2-creERTM with an inducible diphtheria toxin receptor (iDTR) line. Depletion of NG2+ cells changed HSC localization away from arterioles, and switched them into a non-quiescent state. In addition, we found a ~60% reduction in the number of HSCs in the BM. Taken together, these results suggest that periarteriolar NG2+ cells form dormant niches for HSCs and the presence of spatially distinct vascular niches for quiescent and non-quiescent (proliferating) HSCs in the BM.
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| 15. |
Yuya Kunisaki, Bone Marrow Arteriolar Niches Maintain Hematopoietic Stem Cell Quiescence., アメリカ血液学会, 2012.12. |
| 16. |
Yuya Kunisaki, Circadian Adrenergic Regulation of Bone Marrow Endothelial Adhesion Molecule Expression Impacts Progenitor Recruitment and Engraftment Efficiency. , アメリカ血液学会, 2010.12. |
| 17. |
Yuya Kunisaki, Circadian Expression of Endothelial Selectins, Regulated by the Sympathetic Nervous System, Controls Peripheral Leukocyte Homeostasis., アメリカ血液学会, 2008.12. |
