| 1. |
田中 賢, ソフトバイオマテリアル・バイオ界面における水の状態と役割, 新・講座 水の化学 Part 2③, 日本化学会, 化学と教育, 70(7), 348-351 (2022)., 2022.07. |
| 2. |
田中 賢, Nano-biotechnologies on Interfaces, 日本MRSニュース, p. 17, Vol.34 No.1 March 2022., 2022.03. |
| 3. |
田中 賢, 中間水コンセプトによるバイオイナート材料の設計, 2021年度日本バイオマテリアル学会学会賞受賞論文, バイオマテリアルー生体材料ー, 40-1, 14-15 (2022)., 2022.03. |
| 4. |
西田 慶, 田中 賢, 非水溶性高分子の濃縮相が発現する細胞識別性, バイオマテリアルー生体材料ー, 2022.03. |
| 5. |
田中 賢, A03-2 水圏機能材料のバイオ・環境機能開拓, Aquatic Functional Materials News No.3, 2021.07. |
| 6. |
田中 賢, 藤井 義久, 水圏機能材料－水分子に着目して生体適合性材料をデザインする, 現代科学, 2021.07. |
| 7. |
田中 賢, 生物に学ぶ生体適合材料－生体系の水の構造から学ぶ生体適合材料の設計, バイオミメティクス（生体模倣技術）の医療への応用, 医学のあゆみ, Vol.275, No.8, p.918-924 (2020)., 2020.11. |
| 8. |
田中 賢, 次世代高機能材料の動向(6)～バイオ機能材料～, Yano E plus, no.149, p.3-14, 2020年8月号 (2020)., 2020.08. |
| 9. |
田中 賢, 原田 慈久, 瀬戸 秀紀, 材料界面の水の分類と用語について, Aquatic Functional Materials News No.3, 2020.07. |
| 10. |
田中 賢, 医療用材料の設計：バイオ界面水の役割の解明, Annals of Neuroscience and Medical Engineering Meeting in Yamagata, 19, 1-10 (2019)., 2020.06. |
| 11. |
村上大樹, 上田智也, 田中 賢, , Analysis of surface density effect on the anti-thrombogenicity of poly(2-methoxyethyl acrylate) brush, 九州大学中央分析センター研究報告, 37, 11-17 (2019)., 2020.06. |
| 12. |
田中 賢, 特集 医療・介護, 生体親和性材料の開発～生体と材料の相互作用, Seikei-Kako, 31(11), 404-407 (2019)., 2020.06. |
| 13. |
田中 賢, バイオ界面における水分子の状態−中間水の役割は？−, 高分子, 68(6), 311-315 (2019)., 2020.06. |
| 14. |
坂本 遼, 中本 康介, 喜多條 鮎子, 村上 大樹, 平井 晴香, 田中 賢, 周 永全, 山口 敏男, 岡田 重人, , 高電圧水系電池の確立を目指した濃厚水系電解液, 電気化学, 87, 220-226 (2019)., 2020.06. |
| 15. |
村上大樹,北原洋子,上田智也,馬渡なみ,瀬上裕斗,馬場航希,田中 賢, , 高分子／水界面の構造・機能に着目した生体親和性材料の開発,ケミカルエンジニヤリング, 563-569, 64(8) (2019)., 2020.06. |
| 16. |
田中 賢, 界面におけるナノバイオテクノロジー Nanotechnology on interfaces, MRS-J NEWS, p.8, Vol.31, No.1 (2019)., 2020.06. |
| 17. |
田中 賢, 世界初！中間水コンセプトによる血中に含まれるがん細胞の選択的分離・回収技術の創製, MEDTEC INNOVATION, p.16-17 (2019)., 2020.06. |
| 18. |
田中 賢, 医療材料表面の最適化:医療製品と血液の接触面で起こる現象の理解と制御, 透析医療情報誌ねふろん, 6, 72 (2018)., 2020.06. |
| 19. |
田中 賢, The 100th Anniversary of the Birth of Professor Teiji Tsuruta －バイオ界面における水分子の役割の解明と材料設計指針の創成・製品化へ－, Aquatic Functional Materials News No.2, 2020.05. |
| 20. |
田中 賢, 体適合性に優れたソフトバイオマテリアルの設計, 特集 生体適合性材料の新展開, 化学工業, 2019.06. |
| 21. |
田中 賢, 特別な機能をもつ血液透析器の特徴と評価法－生体適合性：in vitro評価法, 透析会誌, 2019.06. |
| 22. |
田中 賢, フロンティアバイオ材料のヘルスケア応用, 特集 医療を支える高機能高分子材料の研究・開発最前線, 工業材料, 2019.06. |
| 23. |
干場隆志, 田中 賢, 培養基板へのタンパク質の吸着現象の変化を利用した細胞機能の制御, バイオマテリアル－生体材料－, 2018.09. |
| 24. |
田中 賢, 化学を介して生物と和む！～分子から個体レベルまで～ 第7回CSJ化学フェスタ バイオ分野のテーマ企画の紹介, 化学と工業, 2018.07. |
| 25. |
田中 賢, 医療材料表面の最適化:医療製品と血液の接触面で起こる現象の理解と制御, 透析医療情報誌ねふろん, 2018.06. |
| 26. |
田中 賢, 生体適合性高分子材料の開発, 機能材料, 38(5), 4-5 (2018)., 2018.05. |
| 27. |
田中 賢, 生体適合性高分子材料の開発, 機能材料, 2018.05. |
| 28. |
中島有利佳, 上野那美, 森澤勇介, 田中賢, 森田成昭, 含水PMEAの減衰全反射遠紫外スペクトルの考察, 日本化学会春季年会講演予稿集(CD-ROM), Vol.98th, p.ROMBUNNO.1G3‐33, 2018.03. |
| 29. |
田中 賢, 生体適合性に優れたソフトバイオマテリアルの設計, 特集 生体適合性材料の新展開, 化学工業, 53, 929-933 (2017)., 2017.12. |
| 30. |
田中 賢, フロンティアバイオ材料のヘルスケア応用, 特集 医療を支える高機能高分子材料の研究・開発最前線, 工業材料, 65(7),56-60 (2017)., 2017.07. |
| 31. |
田中 賢, 血液適合性に優れたソフトバイオマテリアルの界面設計－バイオ界面水の役割の解明と中間水コンセプトによる材料創成－, 生体に優しく, 賢い未来材料 "生体適合性材料"1, , 化学と工業, 70(6), 482-484 (2017)., 2017.06. |
| 32. |
田中 賢, 血液適合性に優れたソフトバイオマテリアルの界面設計－バイオ界面水の役割の解明と中間水コンセプトによる材料創成－, 生体に優しく, 賢い未来材料 "生体適合性材料"1, 化学と工業, 2017.06. |
| 33. |
田中 賢, 特別な機能をもつ血液透析器の特徴と評価法－生体適合性：in vitro評価法, 透析会誌, 50(6), 373-378 (2017)., 2017.05. |
| 34. |
田中 賢, 生体適合性高分子の設計：バイオ界面水の役割と中間水コンセプト, 次世代医療技術開発に貢献する化学工学～「医工連携」における化学工学～, 化学工学, 2017.05. |
| 35. |
田中 賢, 生体適合性高分子の設計：バイオ界面水の役割と中間水コンセプト, 次世代医療技術開発に貢献する化学工学～「医工連携」における化学工学～, 化学工学, 81, 1-4 (2017)., 2017.03. |
| 36. |
田中 賢, バイオマテリアル学の歴史と未来, Journal of Japanese Society for Biomaterials,バイオマテリアル, 34-3, 177 , 2016.10. |
| 37. |
田中 賢, 小林慎吾, 村上大樹, 荒津史裕, 干場隆志, 福島和樹, ＜特集：透析膜up date：生体適合性からみた評価法と特性＞「総論 生体適合性材料の設計概念」, 臨牀透析, Vol32(5), 521-529 (2016)., 2016.07. |
| 38. |
M. Tanaka, K. Sato, E. Kitakami, S. Kobayashi, T. Hoshiba, K. Fukushima, Design of Biocompatible and Biodegradable Polymers Based on the Intermediate Water Concept, Hot Topics, POLYMER JOURNAL: The Most Accessed Papers, 高分子, 161, 2016., 2016.06. |
| 39. |
田中 賢, 抗血栓性コーティング材の設計−水環境で機能する次世代材料, PHARMSTAGE, 2016.06. |
| 40. |
田中 賢, 抗血栓性コーティング材の設計−水環境で機能する次世代材料−, PHARMSTAGE, 16, 51-59 (2016)., 2016.03. |
| 41. |
田中 賢, 高度物理刺激と生体応答−生体応答を支配するバイオ界面因子の解析と先進ヘルスケアマテリアルの創成−, 機械の研究, 68(9), 782-791 (2015)., 2015.09. |
| 42. |
田中 賢, 体内で使用可能な先進医療デバイスを支える「生体親和性ソフトマテリアル」, PHARM STAGE, 15, 29-34 (2015)., 2015.08. |
| 43. |
関根泰斗, S. Asatyas, 丹生 隆, G. Lkhamsuren, 角井杏帆, 佐藤千香子, 森田成昭, 田中 賢, 林 智広, 単分子膜における生体不活性特性のメカニズム:分子集積密度の界面水分子の振る舞いへの影響, 表面科学, 36 (8), 424‐429 (2015), 2015.08. |
| 44. |
田中 賢, 佐藤 一博, 北上 恵理香, 小林 慎吾, 干場 隆志, 福島 和樹, CI-3-5 生体親和性ソフトマテリアルの設計と医療デバイスへの応用(CI-3.界面ナノバイオテクノロジー,依頼シンポジウム,ソサイエティ企画), 電子情報通信学会ソサイエティ大会講演論文集, Vol.2015, No.2, pp."SS-50"-"SS-51", 2015.08. |
| 45. |
森田成昭, 寺西純, 田中賢, ベタイン系高分子の温度依存赤外スペクトルの比較, 高分子学会予稿集(CD-ROM), Vol.64, No.2, p.ROMBUNNO.1W16, 2015.08. |
| 46. |
田中 賢, 中間水コンセプトによる生体親和性高分子の設計, 日本接着学会誌, 51, 423-433 (2015)., 2015.07. |
| 47. |
田中 賢, 中間水コンセプトによる生体親和性高分子の設計, Molecular Electronics and Bioelectronics, 26(1), 15-19,(2015), 2015.04. |
| 48. |
森田成昭, 庄司弘志, 田中賢, 平衡含水したベタイン系高分子にNaClを添加したときのATR‐IRスペクトルの比較, 高分子学会予稿集(CD-ROM), Vol.63, No.2, p.ROMBUNNO.2PD086, 2014.09. |
| 49. |
森田成昭, 田中賢, NaCl添加によるPMEAとP(MPC‐r‐BMA)の水和構造変化の比較, 高分子学会予稿集(CD-ROM), Vol.63, No.1, p.ROMBUNNO.2H10, 2014.05. |
| 50. |
田中 賢, 佐藤 一博, 新規バイオ・医療用高分子の熱分析, 熱測定 = Calorimetry and thermal analysis, Vol.39, No.4, pp.151-157, 2012.10. |
| 51. |
平田豊章, 松野寿生, 藤井義久, 山田悟史, 田中賢, 田中敬二, 水界面における高分子ブレンドの構造形成と生体不活性, 高分子学会予稿集(CD-ROM), Vol.61, No.2, p.ROMBUNNO.2V17, 2012.09. |
| 52. |
田中賢, 林智広, 森田成昭, バイオ界面における水分子の役割の解明と医療高分子への応用, 高分子学会予稿集(CD-ROM), Vol.60, No.2 Disk1, p.ROMBUNNO.2A08IL, 2011.09. |
| 53. |
森田成昭, 田中賢, 北川邦行, 振動分光法を用いた生体適合性高分子の分子構造研究[30]含水高分子への塩添加効果, 高分子学会医用高分子シンポジウム講演要旨集, Vol.40th, pp.99-100, 2011.07. |
| 54. |
田中賢, 林智広, 森田成昭, バイオ界面における水分子の役割は? 次世代医療を切り開く生体親和性材料, 化学, Vol.66, No.5, pp.68-69, 2011.05. |
| 55. |
田中 賢, 水分子の構造制御による血液適合性発現機構の解明, バイオマテリアル : 生体材料 : Journal of Japanese Society for Biomaterials, Vol.28, No.1, pp.34-45, 2010.02. |
| 56. |
James R. McMillan, Masaru Tanaka, Masashi Akiyama, Heather A. Long, Hongjiang Qiao, Yuki Miyamura, Sadaki Yamamoto, Riichiro Abe, Kazuo Kodama, Masatsugu Shimomura, Hiroshi Shimizu, Porous films allow live human keratinocyte delivery and aid wound healing in model mice, JOURNAL OF INVESTIGATIVE DERMATOLOGY, Vol.129, No.12, p.2910, 2009.12. |
| 57. |
Akira Kishi, Masaru Tanaka, Akira Mochizuki, Comparative Study on Water Structures in PolyHEMA and PolyMEA by XRD-DSC Simultaneous Measurement, JOURNAL OF APPLIED POLYMER SCIENCE, 10.1002/app.29127, Vol.111, No.1, pp.476-481, 2009.01, We have found that poly(2-methoxyethylacrylate) (polyMEA) has excellent blood compatibility and proposed that the property is due to freezing bound water in the polymer. This water is defined as that which cold-crystallizes at around -45 degrees C in the heating process of differential scanning calorimetry (DSC). In addition, we have already reported that the water in polyMEA is classified into three types, nonfreezing, freezing bound, and free waters, whereas the water in other polymers is just classified into two types: free and nonfreezing waters. (J Biomed Mater Res 68A, 2004, 684) However, the phenomenon observed by DSC is the enthalpy change and is not a direct evidence for crystallization. To confirm cold-crystallization, a comparative investigation of the thermal and crystallographical properties of water in hydrated polyMEA and poly(2-hydroxyethylmethacrylate) (polyHEMA) as a control was carried out using simultaneous measurements by X-ray diffractometer (XRD) and DSC. In addition, the effect of the water content in the polymers on the properties was studied. As for polyMEA, the finding that XRD crystalline peaks appearing in the heating process were assigned to hexagonal ice indicated cold-crystallization. On the other hand, in the case of polyHEMA, the crystal due to ice was formed only in the cooling process, and during the heating process, the growth of crystal ice was not observed. (C) 2008 Wiley Periodicals, Inc. J Appl Polym Sci 111: 476-481, 2009. |
| 58. |
Akira Kishi, Masaru Tanaka, Akira Mochizuki, Comparative Study on Water Structures in PolyHEMA and PolyMEA by XRD-DSC Simultaneous Measurement, JOURNAL OF APPLIED POLYMER SCIENCE, 10.1002/app.29127, Vol.111, No.1, pp.476-481, 2009.01, We have found that poly(2-methoxyethylacrylate) (polyMEA) has excellent blood compatibility and proposed that the property is due to freezing bound water in the polymer. This water is defined as that which cold-crystallizes at around -45 degrees C in the heating process of differential scanning calorimetry (DSC). In addition, we have already reported that the water in polyMEA is classified into three types, nonfreezing, freezing bound, and free waters, whereas the water in other polymers is just classified into two types: free and nonfreezing waters. (J Biomed Mater Res 68A, 2004, 684) However, the phenomenon observed by DSC is the enthalpy change and is not a direct evidence for crystallization. To confirm cold-crystallization, a comparative investigation of the thermal and crystallographical properties of water in hydrated polyMEA and poly(2-hydroxyethylmethacrylate) (polyHEMA) as a control was carried out using simultaneous measurements by X-ray diffractometer (XRD) and DSC. In addition, the effect of the water content in the polymers on the properties was studied. As for polyMEA, the finding that XRD crystalline peaks appearing in the heating process were assigned to hexagonal ice indicated cold-crystallization. On the other hand, in the case of polyHEMA, the crystal due to ice was formed only in the cooling process, and during the heating process, the growth of crystal ice was not observed. (C) 2008 Wiley Periodicals, Inc. J Appl Polym Sci 111: 476-481, 2009. |
| 59. |
James R. McMillan, Masaru Tanaka, Masashi Akiyama, Heather A. Long, Hongjiang Qiao, Sadaki Yamamoto, Riichiro Abe, Kazuo Kodama, Masatsugu Shimomura, Hiroshi Shimizu, Porous films allow live human skin cell delivery and aid wound healing in model mice, JOURNAL OF DERMATOLOGY, Vol.35, No.9, pp.630-631, 2008.09. |
| 60. |
James R. McMillan, Masaru Tanaka, Masashi Akiyama, Heather A. Long, Hongjiang Qiao, Sadaki Yamamoto, Riichiro Abe, Kazuo Kodama, Masatsugu Shimomura, Hiroshi Shimizu, Porous films allow live human skin cell delivery and aid wound healing in model mice, JOURNAL OF DERMATOLOGY, Vol.35, No.9, pp.630-631, 2008.09. |
| 61. |
Yukako Fukuhira, Masaya Ito, Hiroaki Kaneko, Yoshihiko Sumi, Masaru Tanaka, Sadaaki Yamamoto, Masatsugu Shimomura, Prevention of postoperative adhesions by a novel honeycomb-patterned poly(lactide) film in a rat experimental model, JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 10.1002/jbm.b.31026, Vol.86B, No.2, pp.353-359, 2008.08, Intraperitoneal adhesion is a serious problem concerning abdominal surgery. This study evaluated the performance of a honeycomb-patterned poly(lactide) (HCPLA) film as a physical barrier for preventing postoperative adhesion. HCPLA films were prepared using dioleoylphosphatidylethanolamine (DOPE) or a copolymer of dodecylacrylamide and omega-carboxyhexylacrylamide (CAP) as a surfactant (HCPLA-DOPE and HCPLA-CAP, respectively). In an in vivo adhesion prevention experiment, male Sprague-Dawley rats underwent standard cecum abrasion before midline laparotomy. We placed 2 cm x 2 cm HCPLA and flat films oil the gliding interfaces; untreated rats formed the control group. After 1 week, adhesion was scored from 0 to 4. No significant difference was observed in the scores among groups, but macroscopic differences in adhesion prevention were observed. The adhesive strength of HCPLA-DOPE (18.4 +/- 1.2 g) to skinless chicken breast was significantly higher than that of the flat film (15.2 +/- 0.8 g, p |
| 62. |
Yukako Fukuhira, Masaya Ito, Hiroaki Kaneko, Yoshihiko Sumi, Masaru Tanaka, Sadaaki Yamamoto, Masatsugu Shimomura, Prevention of postoperative adhesions by a novel honeycomb-patterned poly(lactide) film in a rat experimental model, JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 10.1002/jbm.b.31026, Vol.86B, No.2, pp.353-359, 2008.08, Intraperitoneal adhesion is a serious problem concerning abdominal surgery. This study evaluated the performance of a honeycomb-patterned poly(lactide) (HCPLA) film as a physical barrier for preventing postoperative adhesion. HCPLA films were prepared using dioleoylphosphatidylethanolamine (DOPE) or a copolymer of dodecylacrylamide and omega-carboxyhexylacrylamide (CAP) as a surfactant (HCPLA-DOPE and HCPLA-CAP, respectively). In an in vivo adhesion prevention experiment, male Sprague-Dawley rats underwent standard cecum abrasion before midline laparotomy. We placed 2 cm x 2 cm HCPLA and flat films oil the gliding interfaces; untreated rats formed the control group. After 1 week, adhesion was scored from 0 to 4. No significant difference was observed in the scores among groups, but macroscopic differences in adhesion prevention were observed. The adhesive strength of HCPLA-DOPE (18.4 +/- 1.2 g) to skinless chicken breast was significantly higher than that of the flat film (15.2 +/- 0.8 g, p |
| 63. |
森田成昭, 北川邦行, 田中賢, 尾崎幸洋, 振動分光法を用いた生体適合性高分子の分子構造研究[29]poly(2‐hydroxyethyl methacrylate)(PHEMA)フィルムの含水過程時間分解赤外観察, 高分子学会予稿集(CD-ROM), Vol.57, No.1 Disk1, p.2PF182, 2008.05. |
| 64. |
Akinori Tsuruma, Masaru Tanaka, Sadaaki Yamamoto, Masatsugu Shimomura, Control of neural stem cell differentiation on honeycomb films, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 10.1016/j.colsurfa.2007.05.079, Vol.313, pp.536-540, 2008.02, Control of neural stem cell (NSC) differentiation is ongoing interest in neural tissue engineering. Formation of neural networks on various patterned substrates was reported in previous studies. In this study, we cultured NSCs derived from the cerebral cortex of embryonic day-14 mice on honeycomb (HC) films with highly regular pores prepared by casting a polymer solution of water-immiscible solvent under high humidity. The differentiation of NSCs was analyzed by immunostaining for Nestin and MAP2. The differentiation of NSC was controlled for the first time by manipulating the pore size on HC films. The highest suppression of NSC differentiation was observed on HC film with 3 mu m pore specifically. (C) 2007 Elsevier B.V. All rights reserved.. |
| 65. |
Akinori Tsuruma, Masaru Tanaka, Sadaaki Yamamoto, Masatsugu Shimomura, Control of neural stem cell differentiation on honeycomb films, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 10.1016/j.colsurfa.2007.05.079, Vol.313, pp.536-540, 2008.02, Control of neural stem cell (NSC) differentiation is ongoing interest in neural tissue engineering. Formation of neural networks on various patterned substrates was reported in previous studies. In this study, we cultured NSCs derived from the cerebral cortex of embryonic day-14 mice on honeycomb (HC) films with highly regular pores prepared by casting a polymer solution of water-immiscible solvent under high humidity. The differentiation of NSCs was analyzed by immunostaining for Nestin and MAP2. The differentiation of NSC was controlled for the first time by manipulating the pore size on HC films. The highest suppression of NSC differentiation was observed on HC film with 3 mu m pore specifically. (C) 2007 Elsevier B.V. All rights reserved.. |
| 66. |
Yukako Fukuhira, Hiroaki Kaneko, Mika Yamaga, Masaru Tanaka, Sadaaki Yamamoto, Masatsugu Shimomura, Effect of honeycomb-patterned structure on chondrocyte behavior in vitro, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 10.1016/j.colsurfa.2007.05.047, Vol.313, pp.520-525, 2008.02, Chondrocytes were cultured on a honeycomb-patterned poly(lactic acid) (PLA) film in order to evaluate the effects of the honeycomb structure on chondrocytes with regard to cell proliferation, the production of sulphated glycosaminoglycans (sGAG), and morphological behavior. Cell proliferation was evaluated using the alamar blue assay, and sGAG production was spectrophotometrically quantified. Cell morphology was observed by means of optical microscopy (OM) and transmission electron microscopy (TEM). In comparison to the honeycomb-patterned film, the flat film showed significantly higher levels of chondrocyte growth; however, chondrocytes were flattened in a manner similar to that of fibroblasts and produced small amounts of extracellular matrix (ECM). On the contrary, chondrocytes cultured on the honeycomb-patterned film were observed to remain spherical shaped and produce ECM abundantly. The results indicate that the honeycomb-patterned structure reduced the points of attachment for the chondrocytes and that they have the potential to provide chondrocytes with a suitable environment for developing a spherical shape. The hone comb-patterned structure of the PLA film may play an important role in determining chondrocyte behavior and as a scaffold that can be used in tissue engineering. (C) 2007 Elsevier B.V. All rights reserved.. |
| 67. |
Yukako Fukuhira, Hiroaki Kaneko, Mika Yamaga, Masaru Tanaka, Sadaaki Yamamoto, Masatsugu Shimomura, Effect of honeycomb-patterned structure on chondrocyte behavior in vitro, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 10.1016/j.colsurfa.2007.05.047, Vol.313, pp.520-525, 2008.02, Chondrocytes were cultured on a honeycomb-patterned poly(lactic acid) (PLA) film in order to evaluate the effects of the honeycomb structure on chondrocytes with regard to cell proliferation, the production of sulphated glycosaminoglycans (sGAG), and morphological behavior. Cell proliferation was evaluated using the alamar blue assay, and sGAG production was spectrophotometrically quantified. Cell morphology was observed by means of optical microscopy (OM) and transmission electron microscopy (TEM). In comparison to the honeycomb-patterned film, the flat film showed significantly higher levels of chondrocyte growth; however, chondrocytes were flattened in a manner similar to that of fibroblasts and produced small amounts of extracellular matrix (ECM). On the contrary, chondrocytes cultured on the honeycomb-patterned film were observed to remain spherical shaped and produce ECM abundantly. The results indicate that the honeycomb-patterned structure reduced the points of attachment for the chondrocytes and that they have the potential to provide chondrocytes with a suitable environment for developing a spherical shape. The hone comb-patterned structure of the PLA film may play an important role in determining chondrocyte behavior and as a scaffold that can be used in tissue engineering. (C) 2007 Elsevier B.V. All rights reserved.. |
| 68. |
Keiko Arai, Masaru Tanaka, Sadaaki Yamamoto, Masatsup Shimomura, Effect of pore size of honeycomb films on the morphology, adhesion and cytoskeletal organization of cardiac myocytes, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 10.1016/j.colsurfa.2007.04.128, Vol.313, pp.530-535, 2008.02, Cells respond to the circumstances such as structures and chemical composition of scaffolds and cytokine. In this report, the responses of cytoskeleton and spreading of cardiac myocytes to the self-organized honeycomb-patterned films (honeycomb films) of biodegradable poly(epsilon-caprolactone) (PCL) with several pore sizes were investigated. The expression of actin filament of cardiac myocytes was strongly dependent on the pore size ranging from 4 to 13 mu m. Immunofluoreseent labeling of vinculin in cardiac myocytes showed localization of focal contact along the edge of the honeycomb pores. The results indicated that the honeycomb structures and the pore sizes influence the morphology, cytoskeletal organization and focal adhesion of the cardiac myocytes. (C) 2007 Elsevier B.V. All rights reserved.. |
| 69. |
Keiko Arai, Masaru Tanaka, Sadaaki Yamamoto, Masatsup Shimomura, Effect of pore size of honeycomb films on the morphology, adhesion and cytoskeletal organization of cardiac myocytes, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 10.1016/j.colsurfa.2007.04.128, Vol.313, pp.530-535, 2008.02, Cells respond to the circumstances such as structures and chemical composition of scaffolds and cytokine. In this report, the responses of cytoskeleton and spreading of cardiac myocytes to the self-organized honeycomb-patterned films (honeycomb films) of biodegradable poly(epsilon-caprolactone) (PCL) with several pore sizes were investigated. The expression of actin filament of cardiac myocytes was strongly dependent on the pore size ranging from 4 to 13 mu m. Immunofluoreseent labeling of vinculin in cardiac myocytes showed localization of focal contact along the edge of the honeycomb pores. The results indicated that the honeycomb structures and the pore sizes influence the morphology, cytoskeletal organization and focal adhesion of the cardiac myocytes. (C) 2007 Elsevier B.V. All rights reserved.. |
| 70. |
Shusaku Tsukiyama, Michiaki Matsushita, Masaru Tanaka, Hitoshi Tamura, Satoru Todo, Sadaaki Yamamoto, Masatsugu Shimomura, Enhanced cell survival and yield of rat small hepatocytes by honeycomb-patterned films, JAPANESE JOURNAL OF APPLIED PHYSICS, 10.1143/JJAP.47.1429, Vol.47, No.2, pp.1429-1434, 2008.02, Surface designing of substrate to regulate cell adhesion and function in nano and micro scale is a critical issue in biomaterial science. In this study, we describe the fabrication of highly regular patterned porous films (honeycomb-patterned film) formed by a simply casting technique, and the culture of mature hepatocytes and small hepatocytes on the films. The pore size of the honeycomb-patterned films used was 6, 12, and 16 pm. We evaluated the effect of the honeycomb-patterned films on the morphology, cell yield, survival and the differentiated hepatic function (albumin production) of the both hepatocytes. Both hepatocytes attached on the flat films appeared to spread well, showing a typical monolayer morphology. They peeled off from the films at 7 days in culture on the flat films. On the other hand, spreading of the each hepatocytes was restricted on the honeycomb-patterned films at 3 and 7 days in culture. The cell yield and survival of the each hepatocytes increased with increasing culture time. Small hepatocyte on the pore sizes of 16 pm showed the highest cell yield (approximately 3 times). Albumin production of mature hepatocyte on the pore sizes of 16 mu m (224.1.3 +/- 157 ng ml(-1) well(-1) at 1 day in culture, 369.5 +/- 222 ng ml(-1) well(-1) at 3 days in culture) was higher than that of the hepatocytes on the flat films (119.3 +/- 9.3 ng ml(-1) well(-1) at 1 day in culture, 262.8 +/- 47.3 ng ml(-1) well(-1) at 3 days in culture), although that of small hepatocytes on the honeycomb-patterned films (pore size: 16 mu m) was similar on the flat film. These results indicated that both the surface topography and the pore size of the honeycomb-patterned film affected the hepatic metabolic function.. |
| 71. |
Shusaku Tsukiyama, Michiaki Matsushita, Masaru Tanaka, Hitoshi Tamura, Satoru Todo, Sadaaki Yamamoto, Masatsugu Shimomura, Enhanced cell survival and yield of rat small hepatocytes by honeycomb-patterned films, JAPANESE JOURNAL OF APPLIED PHYSICS, 10.1143/JJAP.47.1429, Vol.47, No.2, pp.1429-1434, 2008.02, Surface designing of substrate to regulate cell adhesion and function in nano and micro scale is a critical issue in biomaterial science. In this study, we describe the fabrication of highly regular patterned porous films (honeycomb-patterned film) formed by a simply casting technique, and the culture of mature hepatocytes and small hepatocytes on the films. The pore size of the honeycomb-patterned films used was 6, 12, and 16 pm. We evaluated the effect of the honeycomb-patterned films on the morphology, cell yield, survival and the differentiated hepatic function (albumin production) of the both hepatocytes. Both hepatocytes attached on the flat films appeared to spread well, showing a typical monolayer morphology. They peeled off from the films at 7 days in culture on the flat films. On the other hand, spreading of the each hepatocytes was restricted on the honeycomb-patterned films at 3 and 7 days in culture. The cell yield and survival of the each hepatocytes increased with increasing culture time. Small hepatocyte on the pore sizes of 16 pm showed the highest cell yield (approximately 3 times). Albumin production of mature hepatocyte on the pore sizes of 16 mu m (224.1.3 +/- 157 ng ml(-1) well(-1) at 1 day in culture, 369.5 +/- 222 ng ml(-1) well(-1) at 3 days in culture) was higher than that of the hepatocytes on the flat films (119.3 +/- 9.3 ng ml(-1) well(-1) at 1 day in culture, 262.8 +/- 47.3 ng ml(-1) well(-1) at 3 days in culture), although that of small hepatocytes on the honeycomb-patterned films (pore size: 16 mu m) was similar on the flat film. These results indicated that both the surface topography and the pore size of the honeycomb-patterned film affected the hepatic metabolic function.. |
| 72. |
Masaru Tanaka, Keiko Yoshizawa, Akinori Tsuruma, Hiroshi Sunami, Sadaaki Yamamoto, Masatsugu Shimomura, Formation of hydroxyapatite on a self-organized 3D honeycomb-patterned biodegradable polymer film, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 10.1016/j.colsurfa.2007.05.081, Vol.313, pp.515-519, 2008.02, The design of nano- and microstructures based on self-organization is a key area of research in the search for new biomaterials, and such structures have a variety of potential applications in tissue engineering scaffolds. We have reported a honeycomb-patterned polymer film (honeycomb film) with highly regular pores that is formed by self-organization. In order to utilize the honeycomb film as a scaffold for bone tissue engineering, hydroxyapatite (HA) was formed on the honeycomb film. In this study, we prepared a 3D scaffold comprising HA and a poly(e-caprolactone) honeycomb film in a simulated body fluid (SBF) by changing the degree of hydrophilicity of the film. We investigated the dependence of the amount of HA formed on the length of time for which hydrolysis and soaking in SBF were carried out. Further, we characterized the crystallinity of the HA thus formed. We clarified that the amount of HA formed and its crystallinity were controlled by the length of time for which hydrolysis and soaking of the film were carried out. These HA-deposited honeycomb films, which have the HA microstructure, could serve as a novel scaffold for bone tissue engineering. (C) 2007 Elsevier B.V. All rights reserved.. |
| 73. |
Masaru Tanaka, Keiko Yoshizawa, Akinori Tsuruma, Hiroshi Sunami, Sadaaki Yamamoto, Masatsugu Shimomura, Formation of hydroxyapatite on a self-organized 3D honeycomb-patterned biodegradable polymer film, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 10.1016/j.colsurfa.2007.05.081, Vol.313, pp.515-519, 2008.02, The design of nano- and microstructures based on self-organization is a key area of research in the search for new biomaterials, and such structures have a variety of potential applications in tissue engineering scaffolds. We have reported a honeycomb-patterned polymer film (honeycomb film) with highly regular pores that is formed by self-organization. In order to utilize the honeycomb film as a scaffold for bone tissue engineering, hydroxyapatite (HA) was formed on the honeycomb film. In this study, we prepared a 3D scaffold comprising HA and a poly(e-caprolactone) honeycomb film in a simulated body fluid (SBF) by changing the degree of hydrophilicity of the film. We investigated the dependence of the amount of HA formed on the length of time for which hydrolysis and soaking in SBF were carried out. Further, we characterized the crystallinity of the HA thus formed. We clarified that the amount of HA formed and its crystallinity were controlled by the length of time for which hydrolysis and soaking of the film were carried out. These HA-deposited honeycomb films, which have the HA microstructure, could serve as a novel scaffold for bone tissue engineering. (C) 2007 Elsevier B.V. All rights reserved.. |
| 74. |
Akiko Tanabe, Shigeaki Morita, Masaru Tanaka, Yukihiro Ozaki, Multivariate curve resolution analysis on the multi-component water sorption process into a poly(2-methoxyethyl acrylate) film, APPLIED SPECTROSCOPY, 10.1366/000370208783412555, Vol.62, No.1, pp.46-50, 2008.01, In our previous study, sorption process of water into a biocompatible polymer film, poly(2-methoxyethyl acrylate) (PMEA) was monitored by time-resolved in situ attenuated total reflection infrared (ATR-IR) spectroscopy IS. Morita, et al., Langmuir 23, 3750 (2007)]. In the present study, noisy and heavily overlapped O-H stretching vibrational bands of diffusing water have been analyzed from the series spectra where the spectral shapes change irregularly with time. In spite of these complications, a powerful spectral analysis technique, multivariate curve resolution (MCR) by means of alternating least squares (ALS), yielded smooth and meaningful pure component spectra and detailed kinetic sorption profiles of each component, excluding noise. Ordinary smoothing techniques and Gaussian curve fitting would not achieve these significant results. The quantification of the kinetic parameters such as amplitudes (a) and relaxation time constants (T) is significant for the systematic development of biocompatible materials and also for revealing the mechanisms of biocompatibility of a material. Moreover, the ratios of coefficients of each component at saturation corresponded well to the values obtained by Tanaka et al. measured by gravimetric analysis. This study is the first to report the detailed concentration profile of each water component whose sorption kinetics is discussed comprehensively.. |
| 75. |
Akiko Tanabe, Shigeaki Morita, Masaru Tanaka, Yukihiro Ozaki, Multivariate curve resolution analysis on the multi-component water sorption process into a poly(2-methoxyethyl acrylate) film, Applied Spectroscopy, 10.1366/000370208783412555, Vol.62, No.1, pp.46-50, 2008.01, In our previous study, sorption process of water into a biocompatible polymer film, poly(2-methoxyethyl acrylate) (PMEA) was monitored by time-resolved in situ attenuated total reflection infrared (ATR-IR) spectroscopy [S. Morita, et al., Langmuir 23, 3750 (2007)]. In the present study, noisy and heavily overlapped O-H stretching vibrational bands of diffusing water have been analyzed from the series spectra where the spectral shapes change irregularly with time. In spite of these complications, a powerful spectral analysis technique, multivariate curve resolution (MCR) by means of alternating least squares (ALS), yielded smooth and meaningful pure component spectra and detailed kinetic sorption profiles of each component, excluding noise. Ordinary smoothing techniques and Gaussian curve fitting would not achieve these significant results. The quantification of the kinetic parameters such as amplitudes (a) and relaxation time constants (τ) is significant for the systematic development of biocompatible materials and also for revealing the mechanisms of biocompatibility of a material. Moreover, the ratios of coefficients of each component at saturation corresponded well to the values obtained by Tanaka et al. measured by gravimetric analysis. This study is the first to report the detailed concentration profile of each water component whose sorption kinetics is discussed comprehensively. © 2008 Society for Applied Spectroscopy.. |
| 76. |
Yasumitsu Uraki, Junji Nemoto, Hiroyuki Otsuka, Yutaka Tamai, Junji Sugiyama, Takao Kishimoto, Makoto Ubukata, Hiroshi Yabu, Masaru Tanaka, Masatsugu Shimomura, Honeycomb-like architecture produced by living bacteria, Gluconacetobacter xylinus, CARBOHYDRATE POLYMERS, 10.1016/j.carbpol.2006.08.021, Vol.69, No.1, pp.1-6, 2007.05, Bacterial cellulose (BC)-producing bacterium, Gluconacetobacter xylinus (ATCC53582), was found to move along linear microgrooves of a stripe-patterned cellulosic scaffold. On the basis of this finding, fabrication of honeycomb-patterned BC was attempted by controlling the bacterial movement using a agarose film scaffold with honeycomb-patterned grooves (concave type). The patterned agarose film was prepared by three steps. The first was transcription of a honeycomb-patterned polycaprolactone film template with polydimethyl siloxane. When the bacteria were cultured on the scaffold under atmospheric conditions, only bacterial proliferation was observed. Honeycomb-patterned BC was obtained when cultured under a humid CO2 atmosphere. Electron diffraction and polarized microscopic observation showed that the patterned BC comprised of the well defined cellulose lot microfibrils. As another attempt to fabricate honeycomb-patterned BC, the bacteria were cultured on the patterned cellulose and agarose film with convex type of honeycomb. This culture yielded no honeycomb-patterned BC. Therefore, concave type honeycomb scaffold is more suitable to fabricate honeycomb-patterned BC. (c) 2006 Elsevier Ltd. All rights reserved.. |
| 77. |
Yasumitsu Uraki, Junji Nemoto, Hiroyuki Otsuka, Yutaka Tamai, Junji Sugiyama, Takao Kishimoto, Makoto Ubukata, Hiroshi Yabu, Masaru Tanaka, Masatsugu Shimomura, Honeycomb-like architecture produced by living bacteria, Gluconacetobacter xylinus, CARBOHYDRATE POLYMERS, 10.1016/j.carbpol.2006.08.021, Vol.69, No.1, pp.1-6, 2007.05, Bacterial cellulose (BC)-producing bacterium, Gluconacetobacter xylinus (ATCC53582), was found to move along linear microgrooves of a stripe-patterned cellulosic scaffold. On the basis of this finding, fabrication of honeycomb-patterned BC was attempted by controlling the bacterial movement using a agarose film scaffold with honeycomb-patterned grooves (concave type). The patterned agarose film was prepared by three steps. The first was transcription of a honeycomb-patterned polycaprolactone film template with polydimethyl siloxane. When the bacteria were cultured on the scaffold under atmospheric conditions, only bacterial proliferation was observed. Honeycomb-patterned BC was obtained when cultured under a humid CO2 atmosphere. Electron diffraction and polarized microscopic observation showed that the patterned BC comprised of the well defined cellulose lot microfibrils. As another attempt to fabricate honeycomb-patterned BC, the bacteria were cultured on the patterned cellulose and agarose film with convex type of honeycomb. This culture yielded no honeycomb-patterned BC. Therefore, concave type honeycomb scaffold is more suitable to fabricate honeycomb-patterned BC. (c) 2006 Elsevier Ltd. All rights reserved.. |
| 78. |
森田成昭, 田中賢, 尾崎幸洋, 振動分光法を用いた生体適合性高分子の分子構造研究[28]poly(N‐isopropyl acrylamide)(PNIPA)フィルムの含水過程時間分解赤外観察, 高分子学会予稿集(CD-ROM), Vol.56, No.1 Disk1, p.2H15, 2007.05. |
| 79. |
田邊亜希子, 森田成昭, 田中賢, 尾崎幸洋, 振動分光法を用いた生体適合性高分子の分子構造研究[27]Poly(ethylene glycol)(PEG)フィルムの含水過程時間分解赤外観察, 高分子学会予稿集(CD-ROM), Vol.56, No.1 Disk1, p.2H22R, 2007.05. |
| 80. |
Yong Mei Chen, Masaru Tanaka, Jian Ping Gong, Kazunori Yasuda, Sadaaki Yamamoto, Masatsugu Shimomura, Yoshihito Osada, Platelet adhesion to human umbilical vein endothelial cells cultured on anionic hydrogel scaffolds, BIOMATERIALS, 10.1016/j.biomaterials.2006.12.005, Vol.28, No.10, pp.1752-1760, 2007.04, In this work we describe experiments designed to understand the human platelet adhesion to human umbilical vein endothelial cells (HUVECs) cultured on various kinds of chemically cross-linked anionic hydrogels, which were synthesized by radical polymerization. HUVECs could proliferate to sub-confluent or confluent on poly(acrylic acid) (PAA), poly(2-acrylamido-2-methyl-propane sulfonic acid sodium salt) (PNaAMPS), and poly(sodium p-styrene sulfonate) (PNaSS) gels. The proliferation behavior was not sensitive to the crosslinker concentration of the gels. However, the platelet adhesion on the HUVECs cultured on these gels showed different behavior, as revealed by human platelet adhesion test in static conditions. Only a few platelets adhered on the HUVEC sheets cultured on PNaAMPS gels with 4 and 10 mol% cross-linker concentrations, and completely no platelet adhered on the HUVEC sheets cultured on PNaSS gels with 4 and 10 mol% cross-linker concentrations. On the other hand, a large number of platelets adhered on the HUVECs cultured on PAA gels with 1, 2mol% cross-linker concentrations and PNaAMPS gel with 2mol% cross-linker concentration. Furthermore, the study showed that promote of the glycocalyx of HUVECs with transforming growth factor-beta(1) (TGF-beta(1)) decreased platelet adhesion, and degrade the glycocalyx with heparinase I increased platelet adhesion. The results suggested that the glycocalyx of cultured HUVECs modulates platelet compatibility, and the amount of glycocalyx secreted by HUVECs dependents on the chemical structure and crosslinker concentration of gel scaffolds. This result should be applied to make the hybrid artificial blood vessel composes of gels and endothelial cells with high platelet compatibility. (c) 2006 Elsevier Ltd. All rights reserved.. |
| 81. |
Yong Mei Chen, Masaru Tanaka, Jian Ping Gong, Kazunori Yasuda, Sadaaki Yamamoto, Masatsugu Shimomura, Yoshihito Osada, Platelet adhesion to human umbilical vein endothelial cells cultured on anionic hydrogel scaffolds, BIOMATERIALS, 10.1016/j.biomaterials.2006.12.005, Vol.28, No.10, pp.1752-1760, 2007.04, In this work we describe experiments designed to understand the human platelet adhesion to human umbilical vein endothelial cells (HUVECs) cultured on various kinds of chemically cross-linked anionic hydrogels, which were synthesized by radical polymerization. HUVECs could proliferate to sub-confluent or confluent on poly(acrylic acid) (PAA), poly(2-acrylamido-2-methyl-propane sulfonic acid sodium salt) (PNaAMPS), and poly(sodium p-styrene sulfonate) (PNaSS) gels. The proliferation behavior was not sensitive to the crosslinker concentration of the gels. However, the platelet adhesion on the HUVECs cultured on these gels showed different behavior, as revealed by human platelet adhesion test in static conditions. Only a few platelets adhered on the HUVEC sheets cultured on PNaAMPS gels with 4 and 10 mol% cross-linker concentrations, and completely no platelet adhered on the HUVEC sheets cultured on PNaSS gels with 4 and 10 mol% cross-linker concentrations. On the other hand, a large number of platelets adhered on the HUVECs cultured on PAA gels with 1, 2mol% cross-linker concentrations and PNaAMPS gel with 2mol% cross-linker concentration. Furthermore, the study showed that promote of the glycocalyx of HUVECs with transforming growth factor-beta(1) (TGF-beta(1)) decreased platelet adhesion, and degrade the glycocalyx with heparinase I increased platelet adhesion. The results suggested that the glycocalyx of cultured HUVECs modulates platelet compatibility, and the amount of glycocalyx secreted by HUVECs dependents on the chemical structure and crosslinker concentration of gel scaffolds. This result should be applied to make the hybrid artificial blood vessel composes of gels and endothelial cells with high platelet compatibility. (c) 2006 Elsevier Ltd. All rights reserved.. |
| 82. |
MCMILLAN James R, MCMILLAN James R, AKIYAMA Masashi, TANAKA Masaru, YAMAMOTO Sadaki, GOTO Maki, ABE Riichiro, SAWAMURA Daisuke, SHIMOMURA Masatsugu, SHIMIZU Hiroshi, Small-Diameter Porous Poly ( ε-Caprolactone) Films Enhance Adhesion and Growth of Human Cultured Epidermal Keratinocyte and Dermal Fibroblast Cells, Tissue Engineering, 10.1089/ten.2006.0321, Vol.13, No.4, pp.789-798, 2007.04. |
| 83. |
James R. McMillan, Masashi Akiyama, Masaru Tanaka, Sadaki Yamamoto, Maki Goto, Riichiro Abe, Daisuke Sawamura, Masatsugu Shimomura, Hiroshi Shimizu, Small-diameter porous poly (epsilon-caprolactone) films enhance adhesion and growth of human cultured epidermal keratinocyte and dermal fibroblast cells, TISSUE ENGINEERING, 10.1089/ten.2006.0321, Vol.13, No.4, pp.789-798, 2007.04, Autologous keratinocyte grafts provide clinical benefit by rapidly covering wounded areas, but they are fragile. We therefore developed biocompatible hexagonal-packed porous films with uniform, circular pore sizes to support human keratinocytes and fibroblasts. Cells were cultured on these porous poly (epsilon-calprolactone) films with pore sizes ranging from novel ultra-small 3 mu m to 20 mu m. These were compared with flat (pore-less) films. Cell growth rates, adhesion, migration, and ultrastructural morphology were examined. Human keratinocytes and fibroblasts attached to all films. Furthermore, small-pore (3-5 mu m) films showed the highest levels of cell adhesion and survival and prevented migration into the pores and opposing film surface. Keratinocyte migration over small-pore film surface was inhibited. Keratinocytes optimally attached to 3-mu m-pore films due to a combination of greater pore numbers (porosity), a greater circumference of the pore edge per unit surface area, and greater frequency of flat surface areas for attachment, allowing better cell-substrate and cell-cell attachment and growth. The 3-lmpore size allowed cell-cell communication, together with diffusion of soluble nutrients and factors from the culture medium or wound substrate. These characteristics are considered important in developing grafts for use in the treatment of human skin wounds.. |
| 84. |
TANAKA Masaru, TANAKA Masaru, TAKAYAMA Aiko, ITO Emiko, SUNAMI Hiroshi, SUNAMI Hiroshi, YAMAMOTO Sadaaki, YAMAMOTO Sadaaki, SHIMOMURA Masatsugu, SHIMOMURA Masatsugu, Effect of Pore Size of Self-Organized Honeycomb-Patterned Polymer Films on Spreading, Focal Adhesion, Proliferation, and Function of Endothelial Cells-Organized Honeycomb-Patterned Polymer Films on Spreading, Focal Adhesion, Proliferation, and Function・・・, Journal of Nanoscience and Nanotechnology, 10.1166/jnn.2007.514, Vol.7, No.3, pp.763-772, 2007.03, Effect of Pore Size of Self-Organized Honeycomb-Patterned Polymer Films on Spreading, Focal Adhesion, Proliferation, and Function of Endothelial Cells-Organized Honeycomb-Patterned Polymer Films on Spreading, Focal Adhesion, Proliferation, and Function of Endothelial Cells-Organized Honeycomb-Patterned Polymer Films on Spreading, Focal Adhesion, Proliferation, and Function of Endothelial Cells-Organized Honeycomb-Patterned Polymer Films on Spreading, Focal Adhesion, Proliferation, and Function of Endothelial Cells. |
| 85. |
Masaru Tanaka, Aiko Takayama, Emiko Ito, Hiroshi Sunami, Sadaaki Yamamoto, Masatsugu Shimomura, Effect of pore size of self-organized honeycomb-patterned polymer films on spreading, focal adhesion, proliferation, and function of endothelial cells, JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 10.1166/jnn.2007.514, Vol.7, No.3, pp.763-772, 2007.03, The design of nano- and microstructures based on self-organization is a key area of research in the search for new materials, and it has a variety of potential applications in tissue engineering scaffolds. We have reported a honeycomb-patterned polymer film (honeycomb film) with highly regular pores that is formed by self-organization. This study describes the behavior of vascular endothelial cells (ECs) on honeycomb films with four different pore sizes (5, 9, 12, and 16 mu m) as well as on a flat film. We examined the influence of the honeycomb pattern and pore size on cell behavior. The changes in cell morphologies, actin filaments, vinculin clusters, cell proliferation, and secreted extracellular matrix (ECM) (fibronectin, laminin, type IV collagen, and elastin) production profiles were observed by using optical, fluorescence, and scanning electron microscopy. The ECs that adhered to the flat film showed an elongated morphology with random orientation; the actin filaments and focal adhesions were not conspicuous. On the other hand, the ECs on the honeycomb films exhibited greater spreading and flattening; the degree of spreading of the ECs increased with an increase in the pore size. The actin filaments and focal adhesions appeared conspicuous, and the focal adhesions localized along the edge of the honeycomb pores were distributed over the entire projected cell area. The honeycomb film with a pore size of 5 mu m showed the highest cell proliferation and ECM production profiles. These results suggest that the honeycomb film is a suitable material for designing a new vascular device.. |
| 86. |
Shigeaki Morita, Masaru Tanaka, Yukihiro Ozaki, Time-resolved in situ ATR-IR observations of the process of sorption of water into a poly(2-methoxyethyl acrylate) film, LANGMUIR, 10.1021/la0625998, Vol.23, No.7, pp.3750-3761, 2007.03, A process of water sorption into a biocompatible polymer, poly(2-methoxyethyl acrylate) (PMEA), was investigated by time-resolved, in situ, attenuated total reflection infrared spectroscopy. Evidence for three different types of hydrated water in PMEA, that is, nonfreezing water, freezing bound water, and freezing water, were found. Each hydration structure was elucidated at the functional group level. Nonfreezing water, which never crystallizes, even at -100 degrees C, has a CO center dot center dot center dot H-O type of hydrogen bonding interaction with the carbonyl group of PMEA. Freezing bound water, which crystallizes in a heating process below 0 degrees C, interacts with the methoxy moiety in the PMEA side chain terminal. Freezing water, which crystallizes similar to 0 degrees C, has bulk-water-like structure with an O-H center dot center dot center dot O-H hydrogen bonds network. It has been concluded from the present study that the methoxy moiety in the PMEA side chain terminal plays an important role for the excellent biocompatibility of PMEA.. |
| 87. |
Shigeaki Morita, Masaru Tanaka, Yukihiro Ozaki, Time-resolved in situ ATR-IR observations of the process of sorption of water into a poly(2-methoxyethyl acrylate) film, LANGMUIR, 10.1021/la0625998, Vol.23, No.7, pp.3750-3761, 2007.03, A process of water sorption into a biocompatible polymer, poly(2-methoxyethyl acrylate) (PMEA), was investigated by time-resolved, in situ, attenuated total reflection infrared spectroscopy. Evidence for three different types of hydrated water in PMEA, that is, nonfreezing water, freezing bound water, and freezing water, were found. Each hydration structure was elucidated at the functional group level. Nonfreezing water, which never crystallizes, even at -100 degrees C, has a CO center dot center dot center dot H-O type of hydrogen bonding interaction with the carbonyl group of PMEA. Freezing bound water, which crystallizes in a heating process below 0 degrees C, interacts with the methoxy moiety in the PMEA side chain terminal. Freezing water, which crystallizes similar to 0 degrees C, has bulk-water-like structure with an O-H center dot center dot center dot O-H hydrogen bonds network. It has been concluded from the present study that the methoxy moiety in the PMEA side chain terminal plays an important role for the excellent biocompatibility of PMEA.. |
| 88. |
森田成昭, 田中賢, 尾崎幸洋, 振動分光法を用いた生体適合性高分子の分子構造研究[26]poly(N‐isopropyl acrylamide)フィルムの水和構造, 日本化学会講演予稿集, Vol.87th, No.1, p.123, 2007.03. |
| 89. |
T. Okajima, M. Tanaka, S. Tsukiyama, T. Kadowaki, S. Yamamoto, M. Shimomura, H. Tokumoto, Stress relaxation of HepG2 cells measured by atomic force microscopy, NANOTECHNOLOGY, 10.1088/0957-4484/18/8/084010, Vol.18, No.8, p.084010, 2007.02, Stress relaxation of HepG2 cells was examined with an atomic force microscope ( AFM). In the measurement, a loading force was applied to the cell by an AFM tip, and a time series of the cantilever deflection signal was measured at a fixed position of the cantilever base displacement. The relaxation of the loading force was clearly observed on the HepG2 cells, and was well fitted to a stretched exponential function known as the Kohlrausch-Williams-Watts ( KWW) function, which is empirically employed to represent dispersion processes of the system. The relaxation time and the stretching exponent parameter were estimated to be similar to 0.5 s and 0.4 - 0.6, respectively. The latter indicated that the relaxation observed in HepG2 cells consisted of multiple relaxation processes. Moreover, it was found that the characteristic feature of the relaxation process was not strongly correlated with the elastic properties of the cells.. |
| 90. |
T. Okajima, M. Tanaka, S. Tsukiyama, T. Kadowaki, S. Yamamoto, M. Shimomura, H. Tokumoto, Stress relaxation of HepG2 cells measured by atomic force microscopy, NANOTECHNOLOGY, 10.1088/0957-4484/18/8/084010, Vol.18, No.8, p.084010, 2007.02, Stress relaxation of HepG2 cells was examined with an atomic force microscope ( AFM). In the measurement, a loading force was applied to the cell by an AFM tip, and a time series of the cantilever deflection signal was measured at a fixed position of the cantilever base displacement. The relaxation of the loading force was clearly observed on the HepG2 cells, and was well fitted to a stretched exponential function known as the Kohlrausch-Williams-Watts ( KWW) function, which is empirically employed to represent dispersion processes of the system. The relaxation time and the stretching exponent parameter were estimated to be similar to 0.5 s and 0.4 - 0.6, respectively. The latter indicated that the relaxation observed in HepG2 cells consisted of multiple relaxation processes. Moreover, it was found that the characteristic feature of the relaxation process was not strongly correlated with the elastic properties of the cells.. |
| 91. |
Takaharu Okajima, Masaru Tanaka, Tsubasa Kadowaki, Sadaaki Yamamoto, Masatsugu Shimomura, Hiroshi Tokumoto, Mechanical relaxation of living cells measured by atomic force microscopy, BIOPHYSICAL JOURNAL, p.512A, 2007.01. |
| 92. |
森田成昭, 田中賢, 尾崎幸洋, 振動分光法を用いた生体適合性高分子の分子構造研究[25]赤外スペクトルから予想されるPMEAの水和構造と血液適合性との相関, 日本バイオマテリアル学会大会予稿集, Vol.28th, p.120, 2006.11. |
| 93. |
Sadaaki Yamamoto, Masaru Tanaka, Hiroshi Sunami, Keiko Arai, Aiko Takayama, Shigeko Yamashita, Yuka Morita, Masatsugu Shimomura, Relationship between adsorbed fibronectin and cell adhesion on a honeycomb-patterned film, SURFACE SCIENCE, 10.1016/j.susc.2006.01.085, Vol.600, No.18, pp.3785-3791, 2006.09, Substratum surface morphology plays a vital roles in cellular behavior. Here, we characterized adsorption of fibronectin (Fn) as a typical cell adhesion protein onto honeycomb-patterned films made of poly(epsilon-caprolactone) (PCL) by using atomic force microscopy (AFM) and confocal laser scanning microscopy (CLSM). In order to determine how cells adhere to a honeycomb-patterned film, focal adhesion of cardiac myocytes (CMYs) and endothelial cells (ECs) on the films were studied by using fluorescence labeling of vinculin. Fn adsorbs around the pore edges to form ring-shaped structures. CMYs and ECs adhere onto the honeycomb-patterned films at focal contact points localized around pore edges distributed over the entire cellular surface. The focal contact points on the honeycomb-patterned films correspond well with the adsorption sites of Fn. We suggest that the cell response to honeycomb-patterned films is associated with the adsorption pattern of Fn on the film. (c) 2006 Elsevier B.V. All rights reserved.. |
| 94. |
Sadaaki Yamamoto, Masaru Tanaka, Hiroshi Sunami, Keiko Arai, Aiko Takayama, Shigeko Yamashita, Yuka Morita, Masatsugu Shimomura, Relationship between adsorbed fibronectin and cell adhesion on a honeycomb-patterned film, SURFACE SCIENCE, 10.1016/j.susc.2006.01.085, Vol.600, No.18, pp.3785-3791, 2006.09, Substratum surface morphology plays a vital roles in cellular behavior. Here, we characterized adsorption of fibronectin (Fn) as a typical cell adhesion protein onto honeycomb-patterned films made of poly(epsilon-caprolactone) (PCL) by using atomic force microscopy (AFM) and confocal laser scanning microscopy (CLSM). In order to determine how cells adhere to a honeycomb-patterned film, focal adhesion of cardiac myocytes (CMYs) and endothelial cells (ECs) on the films were studied by using fluorescence labeling of vinculin. Fn adsorbs around the pore edges to form ring-shaped structures. CMYs and ECs adhere onto the honeycomb-patterned films at focal contact points localized around pore edges distributed over the entire cellular surface. The focal contact points on the honeycomb-patterned films correspond well with the adsorption sites of Fn. We suggest that the cell response to honeycomb-patterned films is associated with the adsorption pattern of Fn on the film. (c) 2006 Elsevier B.V. All rights reserved.. |
| 95. |
出口章時, 森田成昭, 田中賢, 尾崎幸洋, 振動分光法を用いた生体適合性高分子の分子構造研究[24]ATR‐IR分光法による生体適性高分子へのタンパク質吸着挙動, 高分子学会予稿集(CD-ROM), Vol.55, No.2 Disk1, p.2PB094, 2006.09. |
| 96. |
田邊亜希子, 森田成昭, 田中賢, 尾崎幸洋, 振動分光法を用いた生体適合性高分子の分子構造研究[23]PMEA含水における時間分解赤外スペクトルの多変量解析, 高分子学会予稿集(CD-ROM), Vol.55, No.2 Disk1, p.2PA093, 2006.09. |
| 97. |
森田成昭, 田中賢, 尾崎幸洋, 溶液と接する高分子フィルムへの分子吸着の時間分解赤外観察, 高分子学会予稿集(CD-ROM), Vol.55, No.2 Disk1, p.1Q19, 2006.09. |
| 98. |
Masaru Tanaka, Kazutaka Nishikawa, Hisashi Okubo, Hirofumi Kamachi, Tomoaki Kawai, Michiaki Matsushita, Satoru Todo, Masatsugu Shimomura, Control of hepatocyte adhesion and function on self-organized honeycomb-patterned polymer film, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 10.1016/j.colsurfa.2005.11.098, Vol.284, pp.464-469, 2006.08, Hepatocytes were cultured on a honeycomb-patterned polymer film (honeycomb film) formed by self-organization in order to investigate the influence of the honeycomb pattern on cell behavior. The changes in cell morphologies and actin filaments were observed by optical, fluorescence, and scanning electron microscopy. Hepatocytes were flattened, and the actin filaments appeared conspicuously in the spreading regions on a flat film. In contrast, the hepatocytes that were cultured on the honeycomb film were observed to form a spherical shape, and the actin filaments were localized inside the edge of the spheroid. The spheroids were observed within several hours after seeding on the honeycomb film; they were attached and the spheroid shape was maintained without any deformation. The spheroids expressed a higher level of liver specific function than the cell monolayers on the flat film. These results suggest that the honeycomb film is a suitable material for tissue engineering scaffolds and biomedical devices. (c) 2005 Elsevier B.V. All rights reserved.. |
| 99. |
Masaru Tanaka, Kazutaka Nishikawa, Hisashi Okubo, Hirofumi Kamachi, Tomoaki Kawai, Michiaki Matsushita, Satoru Todo, Masatsugu Shimomura, Control of hepatocyte adhesion and function on self-organized honeycomb-patterned polymer film, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 10.1016/j.colsurfa.2005.11.098, Vol.284, pp.464-469, 2006.08, Hepatocytes were cultured on a honeycomb-patterned polymer film (honeycomb film) formed by self-organization in order to investigate the influence of the honeycomb pattern on cell behavior. The changes in cell morphologies and actin filaments were observed by optical, fluorescence, and scanning electron microscopy. Hepatocytes were flattened, and the actin filaments appeared conspicuously in the spreading regions on a flat film. In contrast, the hepatocytes that were cultured on the honeycomb film were observed to form a spherical shape, and the actin filaments were localized inside the edge of the spheroid. The spheroids were observed within several hours after seeding on the honeycomb film; they were attached and the spheroid shape was maintained without any deformation. The spheroids expressed a higher level of liver specific function than the cell monolayers on the flat film. These results suggest that the honeycomb film is a suitable material for tissue engineering scaffolds and biomedical devices. (c) 2005 Elsevier B.V. All rights reserved.. |
| 100. |
Hiroshi Sunami, Emiko Ito, Masaru Tanaka, Sadaaki Yamamoto, Masatsugu Shimomura, Effect of honeycomb film on protein adsorption, cell adhesion and proliferation, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 10.1016/j.colsurfa.2005.11.041, Vol.284, pp.548-551, 2006.08, This article describes novel methods for controlling of cell adhesion by using micro porous polymer films. Recently we found the highly ordered micro porous films were formed when poly(e-caprolactone) (PCL) solution was cast on substrates at high atmospheric humidity. The micro porous film has regular honeycomb morphology with a size of 5 mu m per cell (honeycomb film). Endothelial cells grew rapidly on the honeycomb film. After 24 h cell culture, the cell number on honeycomb films was lager than that on PCL flat films. In order to elucidate the effect of honeycomb films as a scaffold for cell culture, the adsorbed proteins on honeycomb films under cell culture condition were observed. After conditioning of the honeycomb film and the flat film in DMEM containing 10% foetal bovine serum (FBS) for 72h at 37 degrees C in 5% CO2 atmosphere, the adsorbed fibronectin-FITC and albumin-Texasred on the honeycomb films was observed by using confocal laser scanning microscope (CLSM). The observation revealed that fibronectin showed site-selective adsorption behavior on the honeycomb film. Albumin adsorbed on the honeycomb film non site-selectively, while fibronectin mainly adsorbed on inside of honeycomb pores. On the flat film, fibronectin was hardly observed. Since the honeycomb film accelerate the adsorption of fibronectin which is a typical protein as a cell adhesion molecule, the film could be a scaffold with excellent cell adhesion proper-ties. (c) 2005 Elsevier B.V. All rights reserved.. |
| 101. |
Hiroshi Sunami, Emiko Ito, Masaru Tanaka, Sadaaki Yamamoto, Masatsugu Shimomura, Effect of honeycomb film on protein adsorption, cell adhesion and proliferation, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 10.1016/j.colsurfa.2005.11.041, Vol.284, pp.548-551, 2006.08, This article describes novel methods for controlling of cell adhesion by using micro porous polymer films. Recently we found the highly ordered micro porous films were formed when poly(e-caprolactone) (PCL) solution was cast on substrates at high atmospheric humidity. The micro porous film has regular honeycomb morphology with a size of 5 mu m per cell (honeycomb film). Endothelial cells grew rapidly on the honeycomb film. After 24 h cell culture, the cell number on honeycomb films was lager than that on PCL flat films. In order to elucidate the effect of honeycomb films as a scaffold for cell culture, the adsorbed proteins on honeycomb films under cell culture condition were observed. After conditioning of the honeycomb film and the flat film in DMEM containing 10% foetal bovine serum (FBS) for 72h at 37 degrees C in 5% CO2 atmosphere, the adsorbed fibronectin-FITC and albumin-Texasred on the honeycomb films was observed by using confocal laser scanning microscope (CLSM). The observation revealed that fibronectin showed site-selective adsorption behavior on the honeycomb film. Albumin adsorbed on the honeycomb film non site-selectively, while fibronectin mainly adsorbed on inside of honeycomb pores. On the flat film, fibronectin was hardly observed. Since the honeycomb film accelerate the adsorption of fibronectin which is a typical protein as a cell adhesion molecule, the film could be a scaffold with excellent cell adhesion proper-ties. (c) 2005 Elsevier B.V. All rights reserved.. |
| 102. |
Akinori Tsuruma, Masaru Tanaka, Sadaaki Yamamoto, Nobuyuki Fukushima, Hiroshi Yabu, Masatsugu Shimomura, Topographical control of neurite extension on stripe-patterned polymer films, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 10.1016/j.colsurfa.2005.11.100, Vol.284, pp.470-474, 2006.08, Controlling cell responses to material surfaces is important for tissue engineering. Topographical property on material surfaces can play a crucial role in directing nerve regeneration. We prepared regular stripe-patterned (groove-ridge pattern) polymer film by self-organization in order to control direction of neurite extension. Neural cells from cerebral cortex of embryonic day-14 mice were cultured on the film coated with poly-L-lysine. Here, we describe a complex and unusual contact guidance dependent on the pattern feature size. The neurites grew perpendicular to wide groove of 12.7 mu m and wide ridge of 4.3 mu m but parallel to narrow grooves (6.1 and 8.4 mu m) and narrow ridge (2.2 and 3.6 mu m). The neurites sprouted parallel to the narrow groove but uniformly on the wide groove. The emersion of neurites was suppressed and the length of neurites was longer compared with on a flat film. These results are of interest to understanding contact guidance and designing scaffold for neural network formation. (c) 2005 Elsevier B.V. All rights reserved.. |
| 103. |
Akinori Tsuruma, Masaru Tanaka, Sadaaki Yamamoto, Nobuyuki Fukushima, Hiroshi Yabu, Masatsugu Shimomura, Topographical control of neurite extension on stripe-patterned polymer films, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 10.1016/j.colsurfa.2005.11.100, Vol.284, pp.470-474, 2006.08, Controlling cell responses to material surfaces is important for tissue engineering. Topographical property on material surfaces can play a crucial role in directing nerve regeneration. We prepared regular stripe-patterned (groove-ridge pattern) polymer film by self-organization in order to control direction of neurite extension. Neural cells from cerebral cortex of embryonic day-14 mice were cultured on the film coated with poly-L-lysine. Here, we describe a complex and unusual contact guidance dependent on the pattern feature size. The neurites grew perpendicular to wide groove of 12.7 mu m and wide ridge of 4.3 mu m but parallel to narrow grooves (6.1 and 8.4 mu m) and narrow ridge (2.2 and 3.6 mu m). The neurites sprouted parallel to the narrow groove but uniformly on the wide groove. The emersion of neurites was suppressed and the length of neurites was longer compared with on a flat film. These results are of interest to understanding contact guidance and designing scaffold for neural network formation. (c) 2005 Elsevier B.V. All rights reserved.. |
| 104. |
森田成昭, 出口章時, 田邊亜希子, 田中賢, 尾崎幸洋, 振動分光法を用いた生体適合性高分子の分子構造研究[18]時間分解赤外分光法による固液界面近傍のその場観察, 高分子学会医用高分子シンポジウム講演要旨集, Vol.35th, pp.81-82, 2006.08. |
| 105. |
出口章時, 森田成昭, 田中賢, 尾崎幸洋, 振動分光学を用いた生体適合性高分子の分子構造研究[16]異なる高分子フィルムに吸着するタンパク質の比較, 高分子学会予稿集(CD-ROM), Vol.55, No.1 Disk1, p.2J02, 2006.05. |
| 106. |
森田成昭, 田中賢, 尾崎幸洋, 振動分光法を用いた生体適合性高分子の分子構造研究[15]水と接するPMEAフィルムの水和構造, 高分子学会予稿集(CD-ROM), Vol.55, No.1 Disk1, p.2J01, 2006.05. |
| 107. |
田邊亜希子, 森田成昭, 田中賢, 尾崎幸洋, 振動分光法を用いた生体適合性高分子の分子構造研究[17]水溶液から高分子フィルムに吸着する低分子の拡散, 高分子学会予稿集(CD-ROM), Vol.55, No.1 Disk1, p.2PF052, 2006.05. |
| 108. |
田中 賢, 鶴間 章典, 角南 寛, 山本 貞明, 下村 政嗣, ハニカムフィルムを用いた組織再生 : 高分子の自己組織化による再生医療用材料の創成, バイオマテリアル : 生体材料 : Journal of Japanese Society for Biomaterials, Vol.24, No.3, pp.152-161, 2006.05. |
| 109. |
Y Fukuhira, E Kitazono, T Hayashi, H Kaneko, M Tanaka, M Shimomura, Y Sumi, Biodegradable honeycomb-patterned film composed of poly(lactic acid) and dioleoylphosphatidylethanolamine, BIOMATERIALS, 10.1016/j.biomaterials.2005.10.019, Vol.27, No.9, pp.1797-1802, 2006.03, Honeycomb-patterned films have been reported to be useful for scaffolds of cell culture in tissue engineering. In the present study, we investigated a new compound, dioleoylphosphatidylethanolamine (DOPE), a naturally derived phospholipid having unsaturated fatty acid moieties, as a surfactant for fabricating honeycomb-patterned poly(D,L-lactide) (PLA) film. Only DOPE among commercially available phospholipids was useful as a surfactant, and it showed good Solubility in PLA/chloroform solution and an excellent property for fabricating honeycomb-patterned film (the concentration of DOPE was from 0.2% to 20% by weight based on the weight of PLA). The pore size of the honeycomb was uniform, and all pores were interconnected with each other. The contact angle of water on the honeycomb-patterned film was affected by the amount of DOPE. Time-of-flight secondary ion mass spectrometer (TOF-SIMS) data suggested that DOPE was concentrated on the surface of the honeycomb-patterned film. To investigate cell proliferation and adhesion on the honeycomb-patterned film, NIH3T3 fibroblast cells were cultured on the film. The NlH3T3 cells adhered well on the honeycomb-patterned PLA film with DOPE (PLA-DOPE) and showed good cell proliferation compared to that on honeycomb-patterned PLA film fabricated with a copolymer (CAP) of dodecylacrylamide and omega-carboxyhexylacrylamide (PLA-CAP).
These results suggest that the honeycomb-patterned PLA-DOPE can be applicable as a scaffold for cells with better profiles in comparison with PLA-CAP. (C) 2005 Elsevier Ltd. All rights reserved.. |
| 110. |
Y Fukuhira, E Kitazono, T Hayashi, H Kaneko, M Tanaka, M Shimomura, Y Sumi, Biodegradable honeycomb-patterned film composed of poly(lactic acid) and dioleoylphosphatidylethanolamine, BIOMATERIALS, 10.1016/j.biomaterials.2005.10.019, Vol.27, No.9, pp.1797-1802, 2006.03, Honeycomb-patterned films have been reported to be useful for scaffolds of cell culture in tissue engineering. In the present study, we investigated a new compound, dioleoylphosphatidylethanolamine (DOPE), a naturally derived phospholipid having unsaturated fatty acid moieties, as a surfactant for fabricating honeycomb-patterned poly(D,L-lactide) (PLA) film. Only DOPE among commercially available phospholipids was useful as a surfactant, and it showed good Solubility in PLA/chloroform solution and an excellent property for fabricating honeycomb-patterned film (the concentration of DOPE was from 0.2% to 20% by weight based on the weight of PLA). The pore size of the honeycomb was uniform, and all pores were interconnected with each other. The contact angle of water on the honeycomb-patterned film was affected by the amount of DOPE. Time-of-flight secondary ion mass spectrometer (TOF-SIMS) data suggested that DOPE was concentrated on the surface of the honeycomb-patterned film. To investigate cell proliferation and adhesion on the honeycomb-patterned film, NIH3T3 fibroblast cells were cultured on the film. The NlH3T3 cells adhered well on the honeycomb-patterned PLA film with DOPE (PLA-DOPE) and showed good cell proliferation compared to that on honeycomb-patterned PLA film fabricated with a copolymer (CAP) of dodecylacrylamide and omega-carboxyhexylacrylamide (PLA-CAP).
These results suggest that the honeycomb-patterned PLA-DOPE can be applicable as a scaffold for cells with better profiles in comparison with PLA-CAP. (C) 2005 Elsevier Ltd. All rights reserved.. |
| 111. |
森田成昭, 田中賢, 尾崎幸洋, 振動分光法を用いた生体適合性高分子の分子構造研究[14]PMEAフィルムに吸着する水の赤外スペクトル, 日本化学会講演予稿集, Vol.86th, No.1, p.305, 2006.03. |
| 112. |
下村 政嗣, 鶴間 章典, 田中 賢, 角南 寛, 山本 貞明, 自己組織化によってパターン化された高分子のバイオメディカル応用, 表面科学 = Journal of The Surface Science Society of Japan, Vol.27, No.3, pp.170-175, 2006.03. |
| 113. |
J Nemoto, Y Uraki, T Kishimoto, Y Sano, R Funada, N Obata, H Yabu, M Tanaka, M Shimomura, Production of mesoscopically patterned cellulose film, BIORESOURCE TECHNOLOGY, 10.1016/j.biortech.2005.01.034, Vol.96, No.17, pp.1955-1958, 2005.11, Honeycomb and stripe patterned films were prepared from cellulose triacetate (CTA)/chloroform solution, as a result of the self-organization of the polymer during evaporation of the solvent. The honeycomb patterned CTA films were prepared by two methods, a direct pattern formation method and a transcription method. The latter method gave a well-organized microporous honeycomb pattern. Both types of patterned CTA films were saponified to yield the corresponding patterned cellulose films. (c) 2005 Elsevier Ltd. All rights reserved.. |
| 114. |
J Nemoto, Y Uraki, T Kishimoto, Y Sano, R Funada, N Obata, H Yabu, M Tanaka, M Shimomura, Production of mesoscopically patterned cellulose film, BIORESOURCE TECHNOLOGY, 10.1016/j.biortech.2005.01.034, Vol.96, No.17, pp.1955-1958, 2005.11, Honeycomb and stripe patterned films were prepared from cellulose triacetate (CTA)/chloroform solution, as a result of the self-organization of the polymer during evaporation of the solvent. The honeycomb patterned CTA films were prepared by two methods, a direct pattern formation method and a transcription method. The latter method gave a well-organized microporous honeycomb pattern. Both types of patterned CTA films were saponified to yield the corresponding patterned cellulose films. (c) 2005 Elsevier Ltd. All rights reserved.. |
| 115. |
森田成昭, 田中賢, 尾崎幸洋, 振動分光法を用いた生体適合性高分子の分子構造研究[12]PMEAに吸着する水の赤外スペクトルの解析, 高分子学会予稿集(CD-ROM), Vol.54, No.2 Disk1, p.2L11, 2005.09. |
| 116. |
森田成昭, 田中賢, 尾崎幸洋, 生体適合性高分子と水との相互作用 吸着水とバルク水を区別するATR‐IR測定法と摂動相関MW2D解析, 分子構造総合討論会講演要旨集(CD-ROM), Vol.2005, p.1A05, 2005.09. |
| 117. |
森田成昭, 出口章時, 田中賢, 尾崎幸洋, 振動分光法を用いた生体適合性高分子の分子構造研究[9]アクリレート系高分子と水との相互作用, 高分子学会医用高分子シンポジウム講演要旨集, Vol.34th, pp.35-36, 2005.08. |
| 118. |
山本 貞明, 田中 賢, 角南 寛, 鶴間 章典, 下村 政嗣, 自己組織化によるポリマー微細加工とナノバイオインターフェース, Molecular electronics and bioelectronics, Vol.16, No.2, pp.69-76, 2005.07. |
| 119. |
Akinori Tsuruma, Masaru Tanaka, Nobuyuki Fukushima, Masatsugu Shimomura, Morphological changes in neurons by self-organized patterned films, e-Journal of Surface Science and Nanotechnology, 10.1380/ejssnt.2005.159, Vol.3, pp.159-164, 2005.05, In tissue engineering, micro/nanofabrication is important to modify substrate surfaces for regulating the attachment and growth of cells. In neuroscience, it is significant for neural regeneration
this involves guiding and extending dendrites and axons by a cell culture scaffold which acts as an extra cellular matrix. In this study, we prepared highly regular porous honeycomb-patterned films by a simple casting technique and cultured neurons to investigate their morphologies on the patterned films. The morphologies of neurons were examined by a scanning electron microscope and a confocal laser scanning microscope. The neurons were round and the neurites extended randomly on the flat film. The patterns influenced the morphologies of neurons. The morphologies of neurons were changed by varying the pore size of the honeycomb- patterned films. The neurites spread along the rims of the honeycomb pattern. These results suggest that the self-organized honeycomb-patterned films are useful biomaterials for neural tissue engineering. © 2005 The Surface Science Society of Japan.. |
| 120. |
Akinori Tsuruma, Masaru Tanaka, Nobuyuki Fukushima, Masatsugu Shimomura, Morphological changes in neurons by self-organized patterned films, e-Journal of Surface Science and Nanotechnology, 10.1380/ejssnt.2005.159, Vol.3, pp.159-164, 2005.05, In tissue engineering, micro/nanofabrication is important to modify substrate surfaces for regulating the attachment and growth of cells. In neuroscience, it is significant for neural regeneration
this involves guiding and extending dendrites and axons by a cell culture scaffold which acts as an extra cellular matrix. In this study, we prepared highly regular porous honeycomb-patterned films by a simple casting technique and cultured neurons to investigate their morphologies on the patterned films. The morphologies of neurons were examined by a scanning electron microscope and a confocal laser scanning microscope. The neurons were round and the neurites extended randomly on the flat film. The patterns influenced the morphologies of neurons. The morphologies of neurons were changed by varying the pore size of the honeycomb- patterned films. The neurites spread along the rims of the honeycomb pattern. These results suggest that the self-organized honeycomb-patterned films are useful biomaterials for neural tissue engineering. © 2005 The Surface Science Society of Japan.. |
| 121. |
H Kitano, T Mori, Y Takeuchi, S Tada, M Gemmei-Ide, Y Yokoyama, M Tanaka, Structure of water incorporated in sulfobetaine polymer films as studied by ATR-FTIR, MACROMOLECULAR BIOSCIENCE, 10.1002/mabi.200400212, Vol.5, No.4, pp.314-321, 2005.04, The structure and hydrogen bonding of water in the vicinity of a thin film of a sulfobetaine copolymer (poly[(N,N-dimethyl-N-(3-sulfopropyl)-3'-methacrylamido-propanaminium inner salt)-ran-(butyl methacrylate)], poly(SPB-r-BMA)), were analyzed with band shapes of O-H stretching of attenuated total reflection infrared (ATR-IR) spectra. The copolymer could be cast as a thin film, of approximate thickness 10 pm, on a ZnSe crystal for the ATR-IR spectroscopy. At an early stage of sorption of water into the polymer film, the O-H stretching band of the IR spectra for the water incorporated in the film was similar to that for free water. This is consistent with the tendency for another zwitterionic polymeric material, poly [(2 -methacryloylox yethylphosphoryleholine)-ran-(butyl methacrylate)] (poly(MPC-r-BMA). It is, however, contradictory to the drastic change in the O-H stretching band for water incorporated into films of polymers such as poly(2-hydroxyethyl methacrylate), poly(methyl methacrylate) and poly(butyl methacrylate). These results suggest that polymers with a zwitterionic structure do not significantly disturb the hydrogen bonding between water molecules incorporated in the thin films. The investigation into the blood-compatibility of both the poly(SPB-r-BMA) and the poly(MPC-r-BMA) films indicate a definite correlation between the blood-compatibility of the polymers and the lack of effect of the polymeric materials on the structure of the incorporated water.. |
| 122. |
H Kitano, T Mori, Y Takeuchi, S Tada, M Gemmei-Ide, Y Yokoyama, M Tanaka, Structure of water incorporated in sulfobetaine polymer films as studied by ATR-FTIR, MACROMOLECULAR BIOSCIENCE, 10.1002/mabi.200400212, Vol.5, No.4, pp.314-321, 2005.04, The structure and hydrogen bonding of water in the vicinity of a thin film of a sulfobetaine copolymer (poly[(N,N-dimethyl-N-(3-sulfopropyl)-3'-methacrylamido-propanaminium inner salt)-ran-(butyl methacrylate)], poly(SPB-r-BMA)), were analyzed with band shapes of O-H stretching of attenuated total reflection infrared (ATR-IR) spectra. The copolymer could be cast as a thin film, of approximate thickness 10 pm, on a ZnSe crystal for the ATR-IR spectroscopy. At an early stage of sorption of water into the polymer film, the O-H stretching band of the IR spectra for the water incorporated in the film was similar to that for free water. This is consistent with the tendency for another zwitterionic polymeric material, poly [(2 -methacryloylox yethylphosphoryleholine)-ran-(butyl methacrylate)] (poly(MPC-r-BMA). It is, however, contradictory to the drastic change in the O-H stretching band for water incorporated into films of polymers such as poly(2-hydroxyethyl methacrylate), poly(methyl methacrylate) and poly(butyl methacrylate). These results suggest that polymers with a zwitterionic structure do not significantly disturb the hydrogen bonding between water molecules incorporated in the thin films. The investigation into the blood-compatibility of both the poly(SPB-r-BMA) and the poly(MPC-r-BMA) films indicate a definite correlation between the blood-compatibility of the polymers and the lack of effect of the polymeric materials on the structure of the incorporated water.. |
| 123. |
H Yabu, M Takebayashi, M Tanaka, M Shimomura, Superhydrophobic and lipophobic properties of self-organized honeycomb and pincushion structures, LANGMUIR, 10.1021/la050013w, Vol.21, No.8, pp.3235-3237, 2005.04, This report describes the simple preparation of superhydrophobic and lipophobic surfaces by self-organization. Microporous polymer films of a fluorinated polymer with hexagonally arranged pores were prepared by casting from solution under humid conditions. Hexagonally packed water microdroplets were formed by evaporative cooling on the surface of the casting solution. After solvent evaporation, a honeycomb-patterned polymer film was formed with the water droplet array acting as a template; the water droplets themselves evaporated soon after the solvent. Two porous polymer layers were stacked vertically, separated by pillars at the hexagon vertexes. After peeling off the top layer using adhesive tape, a pincushion-like structure was obtained. Here, we show that superhydrophobic behavior was achieved, with the maximum contact angle, 170 degrees, observed using these pincushion structures. Theoretical calculations fit the experimental results well. The lipophobic properties of the films are also discussed.. |
| 124. |
H Yabu, M Takebayashi, M Tanaka, M Shimomura, Superhydrophobic and lipophobic properties of self-organized honeycomb and pincushion structures, LANGMUIR, 10.1021/la050013w, Vol.21, No.8, pp.3235-3237, 2005.04, This report describes the simple preparation of superhydrophobic and lipophobic surfaces by self-organization. Microporous polymer films of a fluorinated polymer with hexagonally arranged pores were prepared by casting from solution under humid conditions. Hexagonally packed water microdroplets were formed by evaporative cooling on the surface of the casting solution. After solvent evaporation, a honeycomb-patterned polymer film was formed with the water droplet array acting as a template; the water droplets themselves evaporated soon after the solvent. Two porous polymer layers were stacked vertically, separated by pillars at the hexagon vertexes. After peeling off the top layer using adhesive tape, a pincushion-like structure was obtained. Here, we show that superhydrophobic behavior was achieved, with the maximum contact angle, 170 degrees, observed using these pincushion structures. Theoretical calculations fit the experimental results well. The lipophobic properties of the films are also discussed.. |
| 125. |
田中 賢, 山本 貞明, 下村 政嗣, 生体適合性表面の設計と人工臓器, 人工臓器, Vol.33, No.2, pp.S-81, 2004.09. |
| 126. |
TANAKA M, MOCHIZUKI A, Effect of water structure on blood compatibility－Thermal analysis of water in poly(meth)acrylate－, Journal of Biomedical Materials Research, 10.1002/jbm.a.20088, Vol.68A, No.4, pp.684-695, 2004.03. |
| 127. |
M Tanaka, A Mochizuki, Effect of water structure on blood compatibility - thermal analysis of water in poly(meth)acrylate, JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 10.1002/jbm.a.20088, Vol.68A, No.4, pp.684-695, 2004.03, The purpose of this study is to clarify the main factor causing excellent blood compatibility of poly(2-methoxyethyl acrylate)(PMEA) by the comparison between PMEA and seven PMEA analogous polymers. The polymers have a typical functional group as ester side chains such as methoxyethyl, hydroxyethyl, phenoxyethyl, and alkyl groups. The properties of the polymers relating to water were investigated in terms of contact angle, equilibrium water content (EWC), and thermal analysis by differential scanning calorimetry. The water in PMEA could be classified into three types: nonfreezing water, freezing bound water, and free water while the water in the analogous polymers was classified into just two types: free and nonfreezing waters, regardless of the chemical structure of the side chain. The surface property represented by the contact angle of water corresponded to the content of the bound water (nonfreezing water + freezing bound water). The platelet compatibility in vitro did not depend on the contents of these waters, or on the contact angle. On the basis of the results of this work and the previous work on the platelet compatibility of poly(MEA-co-HEMA) (Tanaka et al. Biomacromolecules 2002;3;36-41), the main factor causing the excellent compatibility of PMEA is discussed. (C) 2004 Wiley Periodicals, Inc.. |
| 128. |
森田成昭, LI G, 田中賢, YE S, 大沢雅俊, 和周波発生(SFG)分光法を用いた高分子薄膜界面の分子構造変化観察, 電気化学秋季大会講演要旨集, Vol.2003, p.132, 2003.09. |
| 129. |
森田成昭, 李桂峰, 田中賢, 叶深, 大沢雅俊, 高分子薄膜の異なる界面における分子構造の評価, 分子構造総合討論会講演要旨集(CD-ROM), Vol.2003, p.2DP11, 2003.09. |
| 130. |
Hiroshi Yabu, Masaru Tanaka, Kuniharu Ijiro, Masatsugu Shimomura, Preparation of honeycomb-patterned polyimide films by self-organization, Langmuir, 10.1021/la034454w, Vol.19, No.15, pp.6297-6300, 2003.07, Microporous polymer films are attractive materials with potential application in the fields of electronics, photonics, and biotechnology. Chemical and thermal stabilities of the microporous polymer films are required for their materials application. Besides preparation by conventional photolithography, we have reported that honeycomb-patterned porous polymer films are prepared by a method utilizing the condensation of small water droplets on solutions of amphiphilic copolymers. Here, we show preparation of honeycomb-patterned microporous films of a thermally and chemically stable material, polyimide. A water-template-assisted honeycomb structure was formed from a polyion complex of polyamic acids and dialkylammonium salt. The pore size of films was controlled by the casting volume of polymer solution. The patterned polyion complex film converted into polyimide by simple chemical treatment, keeping the porous structure. Self-supporting microporous polyimide films are fabricated. The honeycomb-structured film has high thermal and chemical stability like that of conventional cast films of polyimides.. |
| 131. |
H Yabu, M Tanaka, K Ijiro, M Shimomura, Preparation of honeycomb-patterned polyimide films by self-organization, LANGMUIR, 10.1021/la034454w, Vol.19, No.15, pp.6297-6300, 2003.07, Microporous polymer films are attractive materials with potential application in the fields of electronics, photonics, and biotechnology. Chemical and thermal stabilities of the microporous polymer films are required for their materials application. Besides preparation by conventional photolithography, we have reported that honeycomb-patterned porous polymer films are prepared by a method utilizing the condensation of small water droplets on solutions of amphiphilic copolymers. Here, we show preparation of honeycomb-patterned microporous films of a thermally and chemically stable material, polyimide. A water-template-assisted honeycomb structure was formed from a polyion complex of polyamic acids and dialkylammonium salt. The pore size of films was controlled by the casting volume of polymer solution. The patterned polyion complex film converted into polyimide by simple chemical treatment, keeping the porous structure. Self-supporting microporous polyimide films are fabricated. The honeycomb-structured film has high thermal and chemical stability like that of conventional cast films of polyimides.. |
| 132. |
S Ye, S Morita, GF Li, H Noda, M Tanaka, K Uosaki, M Osawa, Structural changes in poly(2-methoxyethyl acrylate) thin films induced by absorption of bisphenol A. An infrared and sum frequency generation (SFG) study, MACROMOLECULES, 10.1021/ma026007d, Vol.36, No.15, pp.5694-5703, 2003.07, The structural changes in poly(2-methoxyethyl acrylate) (PMEA) thin films induced by the absorption of bisphenol A (BPA), which is suspected to be an endocrine disrupter disturbing the hormone balance in a living body, have been investigated by infrared reflection absorption spectroscopy (IRRAS) and sum frequency generation (SFG) measurements. BPA molecules are absorbed in PMEA thin film. By controlling the thickness of the intermediate polystyrene (PS) film between the PMEA and Au substrate, we are able to probe the molecular structure at the different PMEA interfaces of air/PMEA (free) and PMEA/PS (buried) by the SFG measurements. The ether methyl (OCH3) group in the side chain of PMEA at both interfaces has the same vector orientation with respect to Au, i.e., pointing into air at the free interface and pointing into PMEA at the PMEA/PS buried interface. The OCH3 groups at the PMEA interfaces are likely to stand up and that the interfacial ordering on the PMEA is increased by the BPA absorption. The structural changes in the PMEA bulk and its interface induced by BPA absorption and desorption are reversible.. |
| 133. |
S Ye, S Morita, GF Li, H Noda, M Tanaka, K Uosaki, M Osawa, Structural changes in poly(2-methoxyethyl acrylate) thin films induced by absorption of bisphenol A. An infrared and sum frequency generation (SFG) study, MACROMOLECULES, 10.1021/ma026007d, Vol.36, No.15, pp.5694-5703, 2003.07, The structural changes in poly(2-methoxyethyl acrylate) (PMEA) thin films induced by the absorption of bisphenol A (BPA), which is suspected to be an endocrine disrupter disturbing the hormone balance in a living body, have been investigated by infrared reflection absorption spectroscopy (IRRAS) and sum frequency generation (SFG) measurements. BPA molecules are absorbed in PMEA thin film. By controlling the thickness of the intermediate polystyrene (PS) film between the PMEA and Au substrate, we are able to probe the molecular structure at the different PMEA interfaces of air/PMEA (free) and PMEA/PS (buried) by the SFG measurements. The ether methyl (OCH3) group in the side chain of PMEA at both interfaces has the same vector orientation with respect to Au, i.e., pointing into air at the free interface and pointing into PMEA at the PMEA/PS buried interface. The OCH3 groups at the PMEA interfaces are likely to stand up and that the interfacial ordering on the PMEA is increased by the BPA absorption. The structural changes in the PMEA bulk and its interface induced by BPA absorption and desorption are reversible.. |
| 134. |
森田成昭, LI G, 野田浩之, 田中賢, YE S, 大沢雅俊, poly(2-methoxyethyl acrylate)薄膜へのbisphenol Aの吸着 1― QCM およびIRRASによる吸着の追跡―, 高分子学会予稿集, Vol.52, No.4, p.783, 2003.05. |
| 135. |
森田成昭, LI G, 野田浩之, 田中賢, YE S, 大沢雅俊, poly(2‐methoxyethyl acrylate)薄膜へのbisphenolAの吸着 2‐SFG分光法による界面分子構造観察‐, 高分子学会予稿集, Vol.52, No.3, p.486, 2003.05. |
| 136. |
森田成昭, 野田浩之, 田中賢, 大沢雅俊, LI G, YE S, SFG分光法による高分子薄膜の異なる界面における分子構造の観察, 電気化学会大会講演要旨集, Vol.70th, p.78, 2003.03. |
| 137. |
森田成昭, LI G, 野田浩之, 田中賢, 叶深, 大沢雅俊, SFG及びQCM測定による高分子薄膜へのビスフェノールAの吸着過程解析, 日本化学会講演予稿集, Vol.83rd, No.2, p.826, 2003.03. |
| 138. |
M Ide, T Mori, K Ichikawa, H Kitano, M Tanaka, A Mochizuki, H Oshiyama, W Mizuno, Structure of water sorbed into poly (MEA-co-HEMA) films as examined by ATR-IR spectroscopy, LANGMUIR, 10.1021/1a020617p, Vol.19, No.2, pp.429-435, 2003.01, The structure of water sorbed into poly(2-methoxyethyl acrylate) (PMEA), poly(2-hydroxyethyl methacrylate) (PHEMA), and their copolymers (p(MEA/HEMA)) was investigated by attenuated total reflection infrared (ATR-IR) spectroscopy. The extinction coefficient of the OH stretching band of sorbed water (epsilon(OH)) was calculated from the band area obtained by IR measurement and the amount of sorbed water obtained by thermogravimetric analysis. When the polymers contacted with water vapor (relative humidity = similar to55%), the epsilon(OH) values were quite similar in all polymers. On the otherhand, when the polymers contacted with liquid water, the 6011 values were drastically changed by the content of 2-methoxyethyl acrylate (MEA). When the MEA content of the polymers was low ( |
| 139. |
M Ide, T Mori, K Ichikawa, H Kitano, M Tanaka, A Mochizuki, H Oshiyama, W Mizuno, Structure of water sorbed into poly (MEA-co-HEMA) films as examined by ATR-IR spectroscopy, LANGMUIR, 10.1021/1a020617p, Vol.19, No.2, pp.429-435, 2003.01, The structure of water sorbed into poly(2-methoxyethyl acrylate) (PMEA), poly(2-hydroxyethyl methacrylate) (PHEMA), and their copolymers (p(MEA/HEMA)) was investigated by attenuated total reflection infrared (ATR-IR) spectroscopy. The extinction coefficient of the OH stretching band of sorbed water (epsilon(OH)) was calculated from the band area obtained by IR measurement and the amount of sorbed water obtained by thermogravimetric analysis. When the polymers contacted with water vapor (relative humidity = similar to55%), the epsilon(OH) values were quite similar in all polymers. On the otherhand, when the polymers contacted with liquid water, the 6011 values were drastically changed by the content of 2-methoxyethyl acrylate (MEA). When the MEA content of the polymers was low ( |
| 140. |
森田成昭, 野田浩之, 田中賢, 下村政嗣, 大沢雅俊, YE S, 和周波発生(SFG)分光法を用いたpoly(2‐methoxyethyl acrylate)(PMEA)薄膜表面の分子構造研究, 高分子学会予稿集, Vol.51, No.3, p.605, 2002.05. |
| 141. |
M Tanaka, A Mochizuki, T Shiroya, T Motomura, K Shimura, M Onishi, Y Okahata, Study on kinetics of early stage protein adsorption on poly(2-methoxyethylacrylate) (PMEA) surface, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 10.1016/S0927-7757(01)01100-1, Vol.203, No.1-3, pp.195-204, 2002.04, We have already reported that poly(2-methoxyethylacrylate) (PMEA) showed excellent blood compatibility featured by the significantly low adsorption of plasma protein and the low platelet adhesion. In this study, we tried to analyze the adsorption behavior of the plasma proteins (albumin and fibrinogen) on PMEA surface in terms of kinetics in the early stage of the adsorption reaction by using dynamic quartz crystal microbalance (QCM) method. In addition, the conformational changes of the proteins on the polymer surfaces were investigated. It was concluded from the results that the QCM method could be applied to the analysis of the kinetics in such a polymer-protein system. The characteristic of PMEA is that its detachment rate constant k(-1), was higher than those from poly(2-hydroxyethylmethacrylate) (PHEMA) and polypropylene (PP) which were used as references. The degree of the conformational changes of the proteins decreases in the following order; PP > PHEMA > PMEA. This was strongly related to the difference of the detachment rate constant k(-1). (C) 2002 Elsevier Science B.V. All rights reserved.. |
| 142. |
M Tanaka, A Mochizuki, T Shiroya, T Motomura, K Shimura, M Onishi, Y Okahata, Study on kinetics of early stage protein adsorption on poly(2-methoxyethylacrylate) (PMEA) surface, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 10.1016/S0927-7757(01)01100-1, Vol.203, No.1-3, pp.195-204, 2002.04, We have already reported that poly(2-methoxyethylacrylate) (PMEA) showed excellent blood compatibility featured by the significantly low adsorption of plasma protein and the low platelet adhesion. In this study, we tried to analyze the adsorption behavior of the plasma proteins (albumin and fibrinogen) on PMEA surface in terms of kinetics in the early stage of the adsorption reaction by using dynamic quartz crystal microbalance (QCM) method. In addition, the conformational changes of the proteins on the polymer surfaces were investigated. It was concluded from the results that the QCM method could be applied to the analysis of the kinetics in such a polymer-protein system. The characteristic of PMEA is that its detachment rate constant k(-1), was higher than those from poly(2-hydroxyethylmethacrylate) (PHEMA) and polypropylene (PP) which were used as references. The degree of the conformational changes of the proteins decreases in the following order; PP > PHEMA > PMEA. This was strongly related to the difference of the detachment rate constant k(-1). (C) 2002 Elsevier Science B.V. All rights reserved.. |
| 143. |
森田成昭, 野田浩之, 田中賢, YE S, 大沢雅俊, 和周波発生(SFG)分光法を用いた高分子薄膜表面における分子構造の研究, 電気化学会大会講演要旨集, Vol.69th, p.115, 2002.03. |
| 144. |
森田成昭, 野田浩之, 田中賢, YE S, 大沢雅俊, 生体適合性を示すアクリレート系高分子薄膜の表面分子構造, 日本化学会講演予稿集, Vol.81st, No.2, p.798, 2002.03. |
| 145. |
M Tanaka, A Mochizuki, N Ishii, T Motomura, T Hatakeyama, Study of blood compatibility with poly(2-methoxyethyl acrylate). Relationship between water structure and platelet compatibility in poly(2-methoxyethylacrylate-co-2-hydroxyethylmethacrylate), BIOMACROMOLECULES, 10.1021/bm010072y, Vol.3, No.1, pp.36-41, 2002.01, Previously, we reported that poly(2-methoxyethylacrylate) (PMEA) showed excellent blood compatibility and implied that the water structure in PMEA contributed to the blood compatibility. In this study, the relationship between the water structure and the blood compatibility is clarified by studying the influence of the monomer composition of poly(MEA-co-HEMA) on the water structure and the blood compatibility of the copolymers. The water in the polymer was classified into three types: free water, freezing bound water, and nonfreezing water. The polymers with 0-30 mol % of HEMA content had a significant amount of freezing bound water, and the amount decreases greatly when the composition of HEMA exceeded 30 mol %. On the other hand. the amount of other water increased simply with an increase of HEMA content. The evaluation of the platelet compatibility of poly(MEA-co-HEMA) revealed that the adhesion number and the morphological change of platelet on the copolymer surface were least when the HEMA content was 0-20 mol %. These two results strongly suggest that the freezing bound water relates to the platelet compatibility of the polymers.. |
| 146. |
M Tanaka, A Mochizuki, N Ishii, T Motomura, T Hatakeyama, Study of blood compatibility with poly(2-methoxyethyl acrylate). Relationship between water structure and platelet compatibility in poly(2-methoxyethylacrylate-co-2-hydroxyethylmethacrylate), BIOMACROMOLECULES, 10.1021/bm010072y, Vol.3, No.1, pp.36-41, 2002.01, Previously, we reported that poly(2-methoxyethylacrylate) (PMEA) showed excellent blood compatibility and implied that the water structure in PMEA contributed to the blood compatibility. In this study, the relationship between the water structure and the blood compatibility is clarified by studying the influence of the monomer composition of poly(MEA-co-HEMA) on the water structure and the blood compatibility of the copolymers. The water in the polymer was classified into three types: free water, freezing bound water, and nonfreezing water. The polymers with 0-30 mol % of HEMA content had a significant amount of freezing bound water, and the amount decreases greatly when the composition of HEMA exceeded 30 mol %. On the other hand. the amount of other water increased simply with an increase of HEMA content. The evaluation of the platelet compatibility of poly(MEA-co-HEMA) revealed that the adhesion number and the morphological change of platelet on the copolymer surface were least when the HEMA content was 0-20 mol %. These two results strongly suggest that the freezing bound water relates to the platelet compatibility of the polymers.. |
| 147. |
森田成昭, 野田浩之, 田中賢, 叶深, 大沢雅俊, 和周波発生(SFG)分光法によるアクリレート系高分子薄膜の表面分子構造解析, 日本化学会北海道支部研究発表会講演要旨集, Vol.2002, p.61, 2002.01. |
| 148. |
M Tanaka, A Mochizuki, T Motomura, K Shimura, M Onishi, Y Okahata, In situ studies on protein adsorption onto a poly(2-methoxyethylacrylate) surface by a quartz crystal microbalance, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 10.1016/S0927-7757(01)00682-3, Vol.193, No.1-3, pp.145-152, 2001.12, We have reported that poly(2-methoxyethylacrylate) (PMEA) showed excellent blood compatibility though it had a simple chemical structure, and have been making efforts to clarify the reason for the blood compatibility. It is well-known that the adsorption behavior of the protein affects the compatibility. Therefore, the adsorption behaviors of bovine serum albumin (BSA) and human fibrinogen onto the surfaces of PMEA, poly(2-hydroxyethyl methacrylate) (PHEMA) and polypropylene (PP) were investigated by using a quartz crystal microbalance (QCM), where PHEMA and PP were selected as the representatives of hydrophilic and hydrophobic polymers, respectively. Both proteins were observed to adsorb onto all the polymer surfaces according to Langmuir's adsorption isotherm. The maximum adsorption amounts and the apparent association constants of the proteins for PMEA obtained from the isotherm were lower than those for PHEMA and PP. These results suggest that the interaction between PMEA and the proteins is weaker than the interaction with PP and PHEMA. (C) 2001 Elsevier Science B.V. All rights reserved.. |
| 149. |
M Tanaka, A Mochizuki, T Motomura, K Shimura, M Onishi, Y Okahata, In situ studies on protein adsorption onto a poly(2-methoxyethylacrylate) surface by a quartz crystal microbalance, COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 10.1016/S0927-7757(01)00682-3, Vol.193, No.1-3, pp.145-152, 2001.12, We have reported that poly(2-methoxyethylacrylate) (PMEA) showed excellent blood compatibility though it had a simple chemical structure, and have been making efforts to clarify the reason for the blood compatibility. It is well-known that the adsorption behavior of the protein affects the compatibility. Therefore, the adsorption behaviors of bovine serum albumin (BSA) and human fibrinogen onto the surfaces of PMEA, poly(2-hydroxyethyl methacrylate) (PHEMA) and polypropylene (PP) were investigated by using a quartz crystal microbalance (QCM), where PHEMA and PP were selected as the representatives of hydrophilic and hydrophobic polymers, respectively. Both proteins were observed to adsorb onto all the polymer surfaces according to Langmuir's adsorption isotherm. The maximum adsorption amounts and the apparent association constants of the proteins for PMEA obtained from the isotherm were lower than those for PHEMA and PP. These results suggest that the interaction between PMEA and the proteins is weaker than the interaction with PP and PHEMA. (C) 2001 Elsevier Science B.V. All rights reserved.. |
| 150. |
H Kitano, K Ichikawa, M Fukuda, A Mochizuki, M Tanaka, The structure of water sorbed to polymethoxyethylacrylate film as examined by FT-IR spectroscopy, JOURNAL OF COLLOID AND INTERFACE SCIENCE, 10.1006/jcis.2001.7785, Vol.242, No.1, pp.133-140, 2001.10, The state of sorbed water and sorbing processes of water to four kinds of vinyl polymer films were studied by FT-IR. The O-H stretching band of water sorbed to the films gradually increased on contact with a water vapor of 50% relative humidity at 25 degreesC and leveled off. The profile of the O-H stretching band of sorbed water changed with chemical structure of the polymers. Water sorbed to poly(methoxyethylacrylate) (PMEA), for example, had a sharp and large peak at 3625 cm(-1) and a neighboring broader peak with a long slope in the lower frequency region, which resembled the summation of the peaks for water sorbed to poly(methylmethacrylate) (PMMA, two sharp peaks) and poly(vinylmethylether) (PVME, two broader peaks in the lower frequency region) films. The peak frequencies of the sorbed water were consistent with the calculated values for water hydrogen-bonded to the model compounds by using a hybrid density functional method. When water droplets were put on the polymer film, furthermore, the O-H stretching band of water within the polymer matrix was obtained using an attenuated total reflection technique. An O-H profile similar to that of free water was observed for water incorporated within the matrix of PMEA and PVME films, whereas those profiles within the matrix of poly(2-hydroxyethylmethacrylate) (PHEMA) and PMMA films were largely different from that of free water. This might be in accordance with the difference in biocompatibility of four kinds of polymeric materials. The diffusion coefficients of water vapor in these polymer films were also determined by the time-resolved FTIR method. (C) 2001 Academic Press.. |
| 151. |
H Kitano, K Ichikawa, M Fukuda, A Mochizuki, M Tanaka, The structure of water sorbed to polymethoxyethylacrylate film as examined by FT-IR spectroscopy, JOURNAL OF COLLOID AND INTERFACE SCIENCE, 10.1006/jcis.2001.7785, Vol.242, No.1, pp.133-140, 2001.10, The state of sorbed water and sorbing processes of water to four kinds of vinyl polymer films were studied by FT-IR. The O-H stretching band of water sorbed to the films gradually increased on contact with a water vapor of 50% relative humidity at 25 degreesC and leveled off. The profile of the O-H stretching band of sorbed water changed with chemical structure of the polymers. Water sorbed to poly(methoxyethylacrylate) (PMEA), for example, had a sharp and large peak at 3625 cm(-1) and a neighboring broader peak with a long slope in the lower frequency region, which resembled the summation of the peaks for water sorbed to poly(methylmethacrylate) (PMMA, two sharp peaks) and poly(vinylmethylether) (PVME, two broader peaks in the lower frequency region) films. The peak frequencies of the sorbed water were consistent with the calculated values for water hydrogen-bonded to the model compounds by using a hybrid density functional method. When water droplets were put on the polymer film, furthermore, the O-H stretching band of water within the polymer matrix was obtained using an attenuated total reflection technique. An O-H profile similar to that of free water was observed for water incorporated within the matrix of PMEA and PVME films, whereas those profiles within the matrix of poly(2-hydroxyethylmethacrylate) (PHEMA) and PMMA films were largely different from that of free water. This might be in accordance with the difference in biocompatibility of four kinds of polymeric materials. The diffusion coefficients of water vapor in these polymer films were also determined by the time-resolved FTIR method. (C) 2001 Academic Press.. |
| 152. |
K Ichikawa, T Mori, H Kitano, M Fukuda, A Mochizuki, M Tanaka, Fourier transform infrared study on the sorption of water to various kinds of polymer thin films, JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS, 10.1002/polb.1191, Vol.39, No.18, pp.2175-2182, 2001.09, The state of sorbed water and the sorbing processes of water to various polymer thin films were studied with Fourier transform infrared (FTIR) spectroscopy. To prepare the polymer films, we used poly(ethylene glycol)s of different molecular weights and various kinds of vinyl polymers, such as poly(2-methoxyethyl acrylate). The O-H stretching band of water sorbed in the films increased gradually on contact with water vapor at 50% relative humidity and leveled off. When O-H stretching bands of water sorbed to polymer films were compared, the peak positions and profiles of water sorbed to the polymeric materials with the same hydrogen-bonding site were similar. A hybrid density-functional method supported the assignment of the peaks. Furthermore, the diffusion coefficient (D) of water vapor in the polymer films was estimated by time-resolved measurements of the sorbed water at the very initial. stage (0-830 s). It was clearly shown that the D values of water vapor in the polymer materials with a strong hydrogen-bonding site were smaller than those in hydrophobic polymers. The usefulness of the FTIR technique to investigate water sorption to polymer materials was definitely demonstrated. (C) 2001 John Wiley & Sons, Inc.. |
| 153. |
K Ichikawa, T Mori, H Kitano, M Fukuda, A Mochizuki, M Tanaka, Fourier transform infrared study on the sorption of water to various kinds of polymer thin films, JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS, 10.1002/polb.1191, Vol.39, No.18, pp.2175-2182, 2001.09, The state of sorbed water and the sorbing processes of water to various polymer thin films were studied with Fourier transform infrared (FTIR) spectroscopy. To prepare the polymer films, we used poly(ethylene glycol)s of different molecular weights and various kinds of vinyl polymers, such as poly(2-methoxyethyl acrylate). The O-H stretching band of water sorbed in the films increased gradually on contact with water vapor at 50% relative humidity and leveled off. When O-H stretching bands of water sorbed to polymer films were compared, the peak positions and profiles of water sorbed to the polymeric materials with the same hydrogen-bonding site were similar. A hybrid density-functional method supported the assignment of the peaks. Furthermore, the diffusion coefficient (D) of water vapor in the polymer films was estimated by time-resolved measurements of the sorbed water at the very initial. stage (0-830 s). It was clearly shown that the D values of water vapor in the polymer materials with a strong hydrogen-bonding site were smaller than those in hydrophobic polymers. The usefulness of the FTIR technique to investigate water sorption to polymer materials was definitely demonstrated. (C) 2001 John Wiley & Sons, Inc.. |
