| 中島 裕美子（なかじま ゆみこ） | データ更新日：2023.12.30 |
教授 ／
アイソトープ統合安全管理センター
| 1. | T ASATO, Y NAKAJIMA, M NAGAMINE, Y NAKASHIMA, H TAKEI, T MAEHAMA, T YAMASHIRO, M HIGASHI, M NAKAYAMA, K KANAZAWA, CORRELATION BETWEEN THE PROGRESSION OF CERVICAL DYSPLASIA AND THE PREVALENCE OF HUMAN PAPILLOMAVIRUS, JOURNAL OF INFECTIOUS DISEASES, 169, 4, 940-941, 1994.04. |
| 2. | Y Nakajima, K Hashido, K Tsuchida, N Takada, T Shiino, H Maekawa, A novel tripartite structure comprising a mariner-like element and two additional retrotransposons found in the Bombyx mori genome, JOURNAL OF MOLECULAR EVOLUTION, 10.1007/PL00006500, 48, 5, 577-585, 1999.05, A clone of a DNA-mediated mobile element (transposon) corresponding to a mariner-like element (MLE) was obtained by carrying out the polymerase chain reaction with genomic DNA of Bombyx mori using a Hyalophora cecropia MLE sequence as a primer, This clone had a size of about 4.2 kb and, after sequencing, was found to contain an RNA-mediated, shorter retrotransposon named L1Bm, which was in turn integrated with a much longer retrotransposon named BMC1, Thus, the mobile elements made a novel tripartite structure. The BMC1 and L1Bm moieties of the composite structure each contained a 63-bp conserved sequence which was subsequently found to be highly conserved in all BMC1 and L1Bm elements registered so far. We propose that the 63-bp stretch may be a recognition site for a retrotransposition mechanism conducted by a reverse transcriptase and an endonuclease complex. On the basis of this inference, we propose a model that predicts how different types of BMC1 and L1Bm elements are dispersed in the genome. In addition, a phylogenetic tree made from the current and extant BMC1 and L1Bm sequences indicated that these elements can be classified into Subfamilies I and II.. |
| 3. | Funatsuki Kiyomi, Hashido Kazuo, Matsunami Misako, KAMEOKA Yosuke, IWABUCHI Kikuo, TSUKEDA Hohko, TSUCHIDA Kozo, TAKADA Naoko, NAKAJIMA Yumiko, MAEKAWA Hideaki, Rapid identification of Bombyx mori cells using PCR amplification following a direct procedure for genomic DNA preparation, Journal of insect biotechnology and sericology, 10.11416/jibs2001.70.129, 70, 2, 129-136, 2001.06. |
| 4. | T Kusakabe, Y Sugimoto, T Maeda, Y Nakajima, M Miyano, J Nishikawa, S Tone, Y Kawaguchi, K Koga, T Ohyama, Linearization and integration of DNA into cells preferentially occurs at intrinsically curved regions from human LINE-1 repetitive element, GENE, 10.1016/S0378-1119(01)00631-X, 274, 1-2, 271-281, 2001.08, A bent DNA library was constructed from human genomic DNA, from which a new clone belonging to the human LINE-1 sequence family was isolated and characterized. This clone, with a length of 378 base pairs and termed HBC-1 (human bent clone-1), contained an intrinsically occurring curved DNA structure. By permutation analysis, the center of curvature of this fragment was mapped onto the nucleotide position 886 from the 5' terminus of the complete LINE-1 sequence. Reporter plasmids, which contain HBC-1, were effectively integrated into human chromosome, indicating that the bent DNA structure provides a preferential donor site for the integration of human LINE-1 sequences. The present finding may provide an explanation as to why some inactivated LINE-1 sequences on human chromosomes carry the deletion at their 5' termini. (C) 2001 Elsevier Science B.V. All rights reserved.. |
| 5. | Atsuko Ota, Takahiro Kusakabe, Yasushi Sugimoto, Masateru Takahashi, Yumiko Nakajima, Yutaka Kawaguchi, Katsumi Koga, Cloning and characterization of testis-specific tektin in Bombyx mori, Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 10.1016/S1096-4959(02)00153-7, 133, 3, 371-382, 2002.11, A testis-specific cDNA library of Bombyx mori was constructed by an mRNA subtraction technique. Several clones were randomly selected and determined for their nucleotide sequences. One of them, designated as BmTST, contained a 3′-part of an open reading frame homologous to tektin, the protein known to form filamentous polymers in the walls of ciliary and flagellar microtubules. Also isolated was a genomic fragment, which contains the 5′-part of the coding sequence of BmTST and its promoter region. As a whole, the complete open reading frame was found to encode 508 amino acid residues, whose sequence had 28, 28 and 30% identities with the Strongylocentrotus purpuratus tektins A1, B1 and C1, respectively. Expression analysis by reverse transcription polymerase chain reaction with the cDNA and Western blotting with a polyclonal antibody indicated that the BmTST gene was expressed specifically in the testis during sperm maturation. The protein was immunologically detected exclusively in the fraction expected to contain the 9+2 flagellar axonemes of sperms. We infer that the BmTst protein is possibly involved in the spermatogenesis of B. mori. © 2002 Elsevier Science Inc. All rights reserved.. |
| 6. | Hiromu Toma, Jun Kobayashi, Yumi Imada, Takeshi Arakawa, Yumiko Nakajima, Sakhon Laymanivong, Bouakham Vannachone, Khemphabanh Manivong, Samlane Phompida, Yoshiya Sato, Field application and evaluation of a rapid immunochromatographic test for detection of Plasmodium falciparum infection among the inhabitants of Lao PDR, Southeast Asian Journal of Tropical Medicine and Public Health, 34, 1, 43-47, 2003.03, Field application and evaluation of a rapid immunochromatographic test (ICT) for detection of Plasmodium falciparum infection were performed in 13 villages in a southern province of Lao PDR in 1999. More than 2,000 inhabitants, accounting for 61.8% of the total estimated population, were examined. Malaria infection was confirmed in all villages surveyed by ICT and microscopic diagnosis. The positive rates of P. falciparum malaria by microscopy ranged from 9.7% to 59.2% (mean 27.2%), whereas by ICT they were from 11.6% to 64.5% (mean 29.8%). The positive rates by ICT were generally higher in 8 out of 13 villages. However, a significant difference between the positive rates by microscopy and ICT was not observed in all villages. Plasmodium falciparum infection was actually confirmed by microscopy in 84.1% of specimens that tested positive by ICT. The results by ICT were consistent with those of the microscopic diagnosis, the discrepancy of the results was less than 10% (141/2,066). The ICT was falsely-positive in 4.7% and falsely-negative in 2.1% of the test cases. These results showed the efficacy of ICT not only in the diagnosis of the respective cases, but also in the mass-examination in the field.. |
| 7. | K Kurose, M Terai, N Soedarsono, D Rabello, Y Nakajima, RD Burk, M Takagi, Low prevalence of HPV infection and its natural history in normal oral mucosa among volunteers on Miyako Island, Japan, ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS, 10.1016/j.tripleo.2003.12.029, 98, 1, 91-96, 2004.07, Objective. To investigate the prevalence of human papillomavirus (HPV) infection in normal oral mucosa, and to observe the natural history in the oral cavity in oral swab samples collected from healthy volunteers on Miyako Island, Okinawa, Japan. Study design. The prevalence of HPV infection in oral buccal mucosa cell scrapes collected between 2000 and 2002 from a cohort of 668 healthy volunteers was determined. HPV DNA was detected by consensus polymerase chain reaction (PCR) using MY09/MY11 primers followed by direct cycle sequencing. Just over 2 years later the HPV-positive participants were reevaluated. Results. Of the 668 subjects, 662 samples were analyzed for HPV. HPV DNA was detected in 4 (0.6%) specimens. HPV type 16 (HPV16), HPV53, and HPV71, mucosal types, and HPV12, a cutaneous type, were all identified by direct sequencing. In the follow-up survey, the HPV71- and HPV12-positive participants again tested positive, while HPV DNA was not detected in the HPV16- and HPV53-positive participants. Conclusion. The results of this study among healthy individuals from Miyako Island suggest that oral HPV infection is uncommon. In this cohort, HPV71 and HPV12 were persistent, while HPV16 and HPV53 were transient in normal oral mucosa.. |
| 8. | Tomonori Kikuchi, Satoshi Miyazaki, Hitoshi Ohnishi, Junichi Takahashi, Yumiko Nakajima, Kazuki Tsuji, Small queens and big-headed workers in a monomorphic ponerine ant, NATURWISSENSCHAFTEN, 10.1007/s00114-008-0414-8, 95, 10, 963-968, 2008.10, Evolution of caste is a central issue in the biology of social insects. Comparative studies on their morphology so far suggest the following three patterns: (1) a positive correlation between queen-worker size dimorphism and the divergence in reproductive ability between castes, (2) a negative correlation among workers between morphological diversity and reproductive ability, and (3) a positive correlation between queen-worker body shape difference and the diversity in worker morphology. We conducted morphological comparisons between castes in Pachycondyla luteipes, workers of which are monomorphic and lack their reproductive ability. Although the size distribution broadly overlapped, mean head width, head length, and scape length were significantly different between queens and workers. Conversely, in eye length, petiole width, and Weber's length, the size differences were reversed. The allometries (head length/head width, scape length/head width, and Weber's length/head width) were also significantly different between queens and workers. Morphological examinations showed that the body shape was different between queens and workers, and the head part of workers was disproportionately larger than that of queens. This pattern of queen-worker dimorphism is novel in ants with monomorphic workers and a clear exception to the last pattern. This study suggests that it is possible that the loss of individual-level selection, the lack of reproductive ability, influences morphological modification in ants.. |
| 9. | Jae Man Lee, Masateru Takahashi, Hiroaki Mon, Hitoshi Mitsunobu, Katsumi Koga, Yutaka Kawaguchi, Yumiko Nakajima, Takahiro Kusakabe, Construction of gene expression systems in insect cell lines using promoters from the silkworm, Bombyx mori, JOURNAL OF BIOTECHNOLOGY, 10.1016/j.jbiotec.2007.08.033, 133, 1, 9-17, 2008.01, The promoter regions of the Bombyx mori HSC70-4 and B. mori TCTP genes characterized previously were used for the construction of a series of constitutive gene expression systems active in cultured cells. The relative abilities of these promoters were evaluated by comparing those of a silkworm actin A3 (BmActin3) promoter, which is used widely as the first choice. A series of constitutive expression systems constructed were assayed for the transcription efficiency by connecting four reporter cDNAs, firefly luciferase, 3GFP, Ds-Red, and beta-galactosidase gene using the Gateway LR reaction. The insertion of an intron enhancer into the site between the TCTP promoter and gene increased the transcription of the BmTCTP promoter by 10-fold. The insertion of the IE-1 gene and HR3 enhancer to the all three promoters were found to increase the transcription up to 560 times. (C) 2007 Elsevier B.V. All rights reserved.. |
| 10. | 奥間政一郎, 川西祐一, 佐々木健志, 日高道雄, 前川秀彰, 中島裕美子, 亜熱帯に生息する昆虫・クモ類に見出される転移因子マリナー様配列の水平伝播機構の解析, Entomotech, 34, 31-35, 2009.02. |
| 11. | Takaaki Daimon, Masaya Yago, Yu-Feng Hsu, Tsuguru Fujii, Yumiko Nakajima, Ryuhei Kokusho, Hiroaki Abe, Susumu Katsuma, Toru Shimada, Molecular phylogeny, laboratory rearing, and karyotype of the bombycid moth, Trilocha varians, JOURNAL OF INSECT SCIENCE, 10.1673/031.012.4901, 12, 2012.04, This study describes the molecular phylogeny, laboratory rearing, and karyotype of a bombycid moth, Trilocha varians (F. Walker) (Lepidoptera: Bombycidae), which feeds on leaves of Ficus spp. (Rosales: Moraceae). The larvae of this species were collected in Taipei city, Taiwan, and the Ryukyu Archipelago (Ishigaki and Okinawa Islands, Japan). Molecular phylogenetic analyses revealed that T. varians belongs to the subfamily Bombycinae, thus showing a close relationship to the domesticated silkworm Bombyx mori (L.), a lepidopteran model insect. A laboratory method was developed for rearing T. varians and the time required for development from the embryo to adult was determined. From oviposition to adult emergence, the developmental zero was 10.47 degrees C and total effective temperature was 531.2 day-degrees, i.e., approximately 30 days for one generation when reared at 28 degrees C. The haploid of T. varians consisted of n = 26 chromosomes. In highly polyploid somatic nuclei, females showed a large heterochromatin body, indicating that the sex chromosome system in T. varians is WZ/ZZ (female/male). The results of the present study should facilitate the utilization of T. varians as a reference species for B. mori, thereby leading to a greater understanding of the ecology and evolution of bombycid moths.. |
| 12. | Takaaki Daimon, Takeshi Fujii, Masaya Yago, Yu-Feng Hsu, Yumiko Nakajima, Tsuguru Fujii, Susumu Katsuma, Yukio Ishikawa, Toru Shimada, Female sex pheromone and male behavioral responses of the bombycid moth Trilocha varians: comparison with those of the domesticated silkmoth Bombyx mori, NATURWISSENSCHAFTEN, 10.1007/s00114-012-0887-3, 99, 3, 207-215, 2012.03, Analysis of female sex pheromone components and subsequent field trap experiments demonstrated that the bombycid moth Trilocha varians uses a mixture of (E,Z)-10,12-hexadecadienal (bombykal) and (E,Z)-10,12-hexadecadienyl acetate (bombykyl acetate) as a sex pheromone. Both of these components are derivatives of (E,Z)-10,12-hexadecadienol (bombykol), the sex pheromone of the domesticated silkmoth Bombyx mori. This finding prompted us to compare the antennal and behavioral responses of T. varians and B. mori to bombykol, bombykal, and bombykyl acetate in detail. The antennae of T. varians males responded to bombykal and bombykyl acetate but not to bombykol, and males were attracted only when lures contained both bombykal and bombykyl acetate. In contrast, the antennae of B. mori males responded to all the three components. Behavioral analysis showed that B. mori males responded to neither bombykal nor bombykyl acetate. Meanwhile, the wing fluttering response of B. mori males to bombykol was strongly inhibited by bombykal and bombykyl acetate, thereby indicating that bombykal and bombykyl acetate act as behavioral antagonists for B. mori males. T. varians would serve as a reference species for B. mori in future investigations into the molecular mechanisms underlying the evolution of sex pheromone communication systems in bombycid moths.. |
| 13. | Masako Yokoo, Ryosuke Fujita, Yumiko Nakajima, Mamoru Yoshimizu, Hisae Kasai, Shin-ichiro Asano, Hisanori Bando, Mos1 transposon-based transformation of fish cell lines using baculoviral vectors, Biochemical and Biophysical Research Communications, 10.1016/j.bbrc.2013.08.037, 439, 1, 18-22, 2013.09, Drosophila Mos1 belongs to the mariner family of transposons, which are one of the most ubiquitous transposons among eukaryotes. We first determined nuclear transportation of the Drosophila Mos1-EGFP fusion protein in fish cell lines because it is required for a function of transposons. We next constructed recombinant baculoviral vectors harboring the Drosophila Mos1 transposon or marker genes located between Mos1 inverted repeats. The infectivity of the recombinant virus to fish cells was assessed by monitoring the expression of a fluorescent protein encoded in the viral genome. We detected transgene expression in CHSE-214, HINAE, and EPC cells, but not in GF or RTG-2 cells. In the co-infection assay of the Mos1-expressing virus and reporter gene-expressing virus, we successfully transformed CHSE-214 and HINAE cells. These results suggest that the combination of a baculovirus and Mos1 transposable element may be a tool for transgenesis in fish cells. © 2013 Elsevier Inc.. |
| 14. | Shigeki Nakagome, Yumiko Nakajima, Shuhei Mano, Biogeography Revealed by Mariner-Like Transposable Element Sequences via a Bayesian Coalescent Approach, JOURNAL OF MOLECULAR EVOLUTION, 10.1007/s00239-013-9581-0, 77, 3, 64-69, 2013.09, Genetic diversity of natural populations is useful in biogeographical studies. Here, we apply a Bayesian method based on the coalescent model to dating biogeographical events by using published DNA sequences of wild silkworms, Bombyx mandarina, and the domesticated model organisms B. mori, both of which categorized into the order of Lepidoptera, sampled from China, Korea, and Japan. The sequences consist of the BmTNML locus and the flanking intergenic regions. The BmTNML locus is composed of cecropia-type mariner-like element (MLE) with inverted terminal repeats, and three different transposable elements (TE), including L1BM, BMC1 retrotransposons, and BmamaT1, are inserted into the MLE. Based on the genealogy defined by TE insertions/deletions (indels), we estimated times to the most recent common ancestor and these indels events using the flanking, MLE, and indels sequences, respectively. These estimates by using MLE sequences strongly correlated with those by using flanking sequences, implying that cecropia-type MLEs can be used as a molecular clock. MLEs are thought to have transmitted horizontally among different species. By using a pair of published cecropia-type MLE sequences from lepidopteran insect, an emperor moth, and a coral in Ryukyu Islands, we demonstrated dating of horizontal transmission between species which are distantly related but inhabiting geographically close region.. |
| 15. | Goichi Matsumoto, Rie Hirohata, Kousuke Hayashi, Yoko Sugimoto, Eiji Kotani, Junji Shimabukuro, Tomoko Hirano, Yumiko Nakajima, Shin Kawamata, Hajime Mori, Control of angiogenesis by VEGF and endostatin-encapsulated protein microcrystals and inhibition of tumor angiogenesis, BIOMATERIALS, 10.1016/j.biomaterials.2013.10.051, 35, 4, 1326-1333, 2014.01, Encapsulation of cytokines within protein microcrystals (polyhedra) is a promising approach for the stabilization and delivery of therapeutic proteins. Here, we investigate the influence of vascular endothelial growth factor (VEGF) microcrystals and endostatin microcrystals on angiogenesis. VEGF was successfully encapsulated into microcrystals derived from insect cypovirus with overexpression of protein disulfide bond isomerase. VEGF microcrystals were observed to increase the phosphorylation of p42/p44 MAP kinase and to stimulate the proliferation, migration, and network and tube formation of human umbilical vein endothelial cells (HUVECs). Endostatin was also successfully encapsulated into microcrystals. Endostatin microcrystals showed antiangiogenesis activities and inhibited the migration, and network and tube formation of HUVECs. Local administration of endostatin microcrystals in mice inhibited both angiogenesis and tumor growth with clear significant differences between treatment and control groups. Endostatin microcrystals only affected angiogenesis, but had no significant effect on lymphangiogenesis compared to controls. Local therapy using endostatin microcrystals offers a potential approach to achieve sustained therapeutic release of antiangiogenic molecules for cancer treatment. (C) 2013 Elsevier Ltd. All rights reserved.. |
| 16. | Kaori Yamada, Yuichi Kawanishi, Akinori Yamada, Gaku Tokuda, Raj Deep Gurung, Takeshi Sasaki, Yumiko Nakajima, Hideaki Maekawa, A novel cluster of mariner-like elements belonging to mellifera subfamily from spiders and insects: implications of recent horizontal transfer on the South-West Islands of Japan, GENETICA, 10.1007/s10709-014-9762-9, 142, 2, 149-160, 2014.04, Mariner-like elements (MLEs) have been isolated from various eukaryotic genomes and they are divided into 15 subfamilies, including main five subfamilies: mauritiana, cecropia, mellifera/capitata, irritans, and elegans/briggsae. In the present study, MLEs belonging to mellifera subfamily were isolated from various spiders and insects (Hymenoptera and Lepidoptera) inhabiting the South-West Islands of Japan and neighboring regions. MLEs isolated from 15 different species formed a distinct novel cluster in mellifera subfamily. MLEs obtained from three different species [i.e., the bee Amegilla senahai subflavescens (Amsmar1), the wasp Campsomeris sp. (Casmar1), and the swallowtail butterfly Pachliopta aristolochiae (Paamar1)] contained an intact open reading frame that encoded a putative transposase. These transposases exhibited high similarity of 97.9 % among themselves. In case of Casmar1, the presence of an intact ORF was found in high frequencies (i.e., 11 out of 12 clones). In addition, these transposases also showed the presence of a terminal inverted repeat-binding motif, DD(34)D and two highly conserved amino acid motifs, (W/L)(I/L)PHQL and YSP(D/N)L(A/S)P. These two motifs differed from previously known motifs, WVPHEL and YSPDLAP. MLEs isolated from these three different species may have been inserted into their genomes by horizontal transfer. Furthermore, the presence of an intact ORF suggests that they are still active in habitats along these isolated islands.. |
| 17. | Junji Shimabukuro, Ayako Yamaoka, Ken-ichi Murata, Eiji Kotani, Tomoko Hirano, Yumiko Nakajima, Goichi Matsumoto, Hajime Mori, 3D co-cultures of keratinocytes and melanocytes and cytoprotective effects on keratinocytes against reactive oxygen species by insect virus-derived protein microcrystals, MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 10.1016/j.msec.2014.05.013, 42, 64-69, 2014.09, Stable protein microcrystals called polyhedra are produced by certain insect viruses. Cytokines, such as fibroblast growth factors (FGFs), can be immobilized within polyhedra. Here, we investigated three-dimensional (3D) co-cultures of keratinocytes and melanocytes on collagen gel containing FGF-2 and FGF-7 polyhedra. Melanocytes were observed to reside at the base of the 3D cell culture and melanin was also typically observed in the lower layer. The 3D cell culture model with FGF-2 and FGF-7 polyhedra was a useful in vitro model of the epidermis due to effective melanogenesis, proliferation and differentiation of keratinocytes. FGF-7 polyhedra showed a potent cytoprotective effect when keratinocytes were treated with menadione, which is a generator of reactive oxygen species. The cytoprotective effect was activated by the inositol triphosphate kinase-Akt pathway leading to upregulation of the antioxidant enzymes superoxide dismutase and peroxiredoxin 6. (C) 2014 Elsevier B.V. All rights reserved.. |
| 18. | Matsuo Kazunori, Hirose Yoshimi, Yokoyama Takeshi, Nakajima Yumiko, Hsu Yu-Feng, Banno Yutaka, Discovery of a New Species of Telenomus (Hymenoptera: Scelionidae) Parasitic on Eggs of Bombyx mandarina and Bombyx mori (Lepidoptera: Bombycidae) in Japan and Taiwan, JOURNAL OF INSECT SCIENCE, 10.1093/jisesa/iey072, 18, 4, 2018.08. |
| 19. | 吉谷 悠, 藤淵 俊王, 中島 裕美子, ディジタルカメラを用いたガンマ線イメージングの基礎検討, 日本放射線技術学会総会学術大会予稿集, 76回, 274-274, 2020.03. |
| 20. | Hiroshi Yoshitani, Toshioh Fujibuchi, Yumiko Nakajima, Basic study of mobile;gamma;ray imaging;using;a;digital camera andscintillator, Biomed. Phys. Eng. Express, 7, 1-6, 2021.04. |
| 21. | Hiroshi Yoshitani, Toshioh Fujibuchi, Yumiko Nakajima, Basic study of mobile gamma ray imaging using a digital camera and scintillator, Biomedical Physics & Engineering Express, 7, 1-6, 2021.03, Gamma cameras are used in nuclear medicine examinations involving radioisotopes; however, they do not provide real-time feedback. We propose a real-time imaging method based on a commercially available digital camera and a scintillator array to provide simple and accurate measurements of radioisotope accumulation and contamination. We evaluate the sensitivity and resolution of the proposed device using X-rays as a proxy for gamma-rays. The performance of the device is demonstrated using PENTAX KP and ORCA-spark C11440-36U digital cameras. A caesium iodide scintillator array is irradiated with X-rays, with the state of light emission confirmed using live view images. The pixel value is evaluated as a function of dose rate. Furthermore, we investigate the state of light emission in response to amplifying the light signal using an image intensifier. For the PENTAX KP, luminescence is observable for a dose rate of approximately 10 mSv/h, which changes to 2.1 mSv/h when an image intensifier is used. Notably, the ORCA-spark detected emission at a low dose rate of 0.06 mSv/h. However, using an image intensifier resulted in noisier images. Therefore, although the ORCA-spark can observe luminescence at a suitable predicted dose rate for application in nuclear medicine examinations, a collimator is required to control the spread of gamma rays. However, as this causes the sensitivity to decrease, increasing the amount of light emitted by the scintillator and improving the sensitivity of the camera is vital.. |
| 22. | Kazunori Matsuo, Yoshimi Hirose, Takeshi Yokoyama, Yumiko Nakajima, Yu-Feng Hsu, Yutaka Banno, Discovery of a New Species of Telenomus (Hymenoptera:Scelionidae) Parasitic on Eggs of Bombyx mandarina and Bombyx mori (Lepidoptera: Bombycidae) in Japan and Taiwan , Journal of Insect Science, 10.1093/jisesa/iey072 Research, 18, 4:10, 1-7, 2018.08, We reared a Telenomus species from eggs of Bombyx mandarina (Moore) (Lepidoptera: Bombycidae) and Bombyx mori (Linnaeus) (Lepidoptera: Bombycidae) in Japan, and from eggs of B. mandarina in Taiwan. Morphological examination revealed that this Telenomus species is new to science. In this article, we describe it as Telenomus moricolus Matsuo et Hirose, sp. nov. Because B. mandarina is considered to be an ancestor of B. mori, a domestic insect, it is reasonable to assume that B. mandarina is an original host of T. moricolus. This is the second discovery of an egg parasitoid attacking wild and domesticated silkworms, following the first discovery of T. theophilae, a Chinese species. The significance of the discovery of T. moricolus is discussed in relation to examining the effects of host-insect domestication on egg parasitism.. |
| 23. | 平田哲平, 大松 勉, 中島 裕美子, 辰己賢一, 水谷哲也, 玉城史朗, 家畜感染症に関する数理疫学モデルの構築と防疫情報システムの設計, 電気情報通信学会, 114, 290, 47-52, 2015.03. |
| 24. | Shigeki Nakagome, Yumiko Nakajima, Shuhei Mano, Biogeography revealed by mariner-like transposable element sequences via a bayesian coalescent approach, Journal of Molecular Evolution, 10.1007/s00239-013-9581-0, 77, 3, 64-69, 2013.09, [URL], Genetic diversity of natural populations is useful in biogeographical studies. Here, we apply a Bayesian method based on the coalescent model to dating biogeographical events by using published DNA sequences of wild silkworms, Bombyx mandarina, and the domesticated model organisms B. mori, both of which categorized into the order of Lepidoptera, sampled from China, Korea, and Japan. The sequences consist of the BmTNML locus and the flanking intergenic regions. The BmTNML locus is composed of cecropia-type mariner-like element (MLE) with inverted terminal repeats, and three different transposable elements (TE), including L1BM, BMC1 retrotransposons, and BmamaT1, are inserted into the MLE. Based on the genealogy defined by TE insertions/deletions (indels), we estimated times to the most recent common ancestor and these indels events using the flanking, MLE, and indels sequences, respectively. These estimates by using MLE sequences strongly correlated with those by using flanking sequences, implying that cecropia-type MLEs can be used as a molecular clock. MLEs are thought to have transmitted horizontally among different species. By using a pair of published cecropia-type MLE sequences from lepidopteran insect, an emperor moth, and a coral in Ryukyu Islands, we demonstrated dating of horizontal transmission between species which are distantly related but inhabiting geographically close region.. |
| 25. | Junji Shimabukuro, Ayako Yamaoka, Ken-ichi Murata, Eiji Kotani, Tomoko Hirano, Yumiko Nakajima, Goichi Matsumoto, Hajime Mori, 3D co-cultures of keratinocytes and melanocytes and cytoprotective effects on keratinocytes against reactive oxygen species by insect virus-derived protein microcrystals, Materials Science and Engineering: C , 10.1016/j.msec.2014.05.013, 42, 1, 64-69, 2014.09, Stable protein microcrystals called polyhedra are produced by certain insect viruses. Cytokines, such as fibroblast growth factors (FGFs), can be immobilized within polyhedra. Here, we investigated three-dimensional (3D) co-cultures of keratinocytes and melanocytes on collagen gel containing FGF-2 and FGF-7 polyhedra. Melanocytes were observed to reside at the base of the 3D cell culture and melanin was also typically observed in the lower layer. The 3D cell culture model with FGF-2 and FGF-7 polyhedra was a useful in vitro model of the epidermis due to effective melanogenesis, proliferation and differentiation of keratinocytes. FGF-7 polyhedra showed a potent cytoprotective effect when keratinocytes were treated with menadione, which is a generator of reactive oxygen species. The cytoprotective effect was activated by the inositol triphosphate kinase–Akt pathway leading to upregulation of the antioxidant enzymes superoxide dismutase and peroxiredoxin 6.. |
| 26. | Kaori Yamada, Yuichi Kawanish, Gaku Tokuda, Akinori Yamada, Raj Deep Gurung, Takeshi Sasaki, Yumiko Nakajima, Hideaki Maekawa, A novel cluster of mariner-like elements belonging to mellifera subfamily from spiders and insects: implications of recent horizontal transfer on the South-West Islands of Japan, Genetica, 10.1007/s10709-014-9762-9, 142, 2, 149-160, 2014.04, Mariner-like elements (MLEs) have been isolated from various eukaryotic genomes and they are divided into 15 subfamilies, including main five subfamilies: mauritiana, cecropia, mellifera/capitata, irritans, and elegans/briggsae. In the present study, MLEs belonging to mellifera subfamily were isolated from various spiders and insects (Hymenoptera and Lepidoptera) inhabiting the South-West Islands of Japan and neighboring regions. MLEs isolated from 15 different species formed a distinct novel cluster in mellifera subfamily. MLEs obtained from three different species [i.e., the bee Amegilla senahai subflavescens (Amsmar1), the wasp Campsomeris sp. (Casmar1), and the swallowtail butterfly Pachliopta aristolochiae (Paamar1)] contained an intact open reading frame that encoded a putative transposase. These transposases exhibited high similarity of 97.9 % among themselves. In case of Casmar1, the presence of an intact ORF was found in high frequencies (i.e., 11 out of 12 clones). In addition, these transposases also showed the presence of a terminal inverted repeat-binding motif, DD(34)D and two highly conserved amino acid motifs, (W/L)(I/L)PHQL and YSP(D/N)L(A/S)P. These two motifs differed from previously known motifs, WVPHEL and YSPDLAP. MLEs isolated from these three different species may have been inserted into their genomes by horizontal transfer. Furthermore, the presence of an intact ORF suggests that they are still active in habitats along these isolated islands.. |
| 27. | Goichi Matsumoto, Rie Hirohata, Kousuke Hayashi, Yoko Sugimoto, Eiji Kotani, Junji Shimabukuro, Tomoko Hirano, Yumiko Nakajima, Shin Kawamata, Hajime Mori, Control of angiogenesis by VEGF and endostatin-encapsulated protein microcrystals and inhibition of tumor angiogenesis, Biomaterials , 10.1016/j.biomaterials.2013.10.051, 35, 4, 1326-1333, 2014.01, Encapsulation of cytokines within protein microcrystals (polyhedra) is a promising approach for the stabilization and delivery of therapeutic proteins. Here, we investigate the influence of vascular endothelial growth factor (VEGF) microcrystals and endostatin microcrystals on angiogenesis. VEGF was successfully encapsulated into microcrystals derived from insect cypovirus with overexpression of protein disulfide bond isomerase. VEGF microcrystals were observed to increase the phosphorylation of p42/p44 MAP kinase and to stimulate the proliferation, migration, and network and tube formation of human umbilical vein endothelial cells (HUVECs). Endostatin was also successfully encapsulated into microcrystals. Endostatin microcrystals showed antiangiogenesis activities and inhibited the migration, and network and tube formation of HUVECs. Local administration of endostatin microcrystals in mice inhibited both angiogenesis and tumor growth with clear significant differences between treatment and control groups. Endostatin microcrystals only affected angiogenesis, but had no significant effect on lymphangiogenesis compared to controls. Local therapy using endostatin microcrystals offers a potential approach to achieve sustained therapeutic release of antiangiogenic molecules for cancer treatment. |
| 28. | Masako Yokoo, Ryosuke Fujita, Yumiko Nakajima, Mamoru Yoshimizu, Hisae Kasai, Shin-ichiro Asano, Hisanori Bando, Mos1 transposon-based transformation of fish cell lines using baculoviral vectors, Biochemical and biophysical research communications, 10.1016/j.bbrc.2013.08.037, 439, 11, 18-22, 2013.09, Drosophila Mos1 belongs to the mariner family of transposons, which are one of the most ubiquitous transposons among eukaryotes. We first determined nuclear transportation of the Drosophila Mos1-EGFP fusion protein in fish cell lines because it is required for a function of transposons. We next constructed recombinant baculoviral vectors harboring the Drosophila Mos1 transposon or marker genes located between Mos1 inverted repeats. The infectivity of the recombinant virus to fish cells was assessed by monitoring the expression of a fluorescent protein encoded in the viral genome. We detected transgene expression in CHSE-214, HINAE, and EPC cells, but not in GF or RTG-2 cells. In the co-infection assay of the Mos1-expressing virus and reporter gene-expressing virus, we successfully transformed CHSE-214 and HINAE cells. These results suggest that the combination of a baculovirus and Mos1 transposable element may be a tool for transgenesis in fish cells.. |
| 29. | Takaaki Daimon, Takeshi Fujii, Masaya Yago, Yu-Feng Hsu, Yumiko Nakajima, Tsuguru Fujii, Susumu Katsuma, Yukio Ishikawa, Toru Shimada, Female sex pheromone and male behavioral responses of the bombycid moth Trilocha varians comparison with those of the domesticated silkmoth Bombyx mori. , Naturwissenschaften , 10.1007/s00114-012-0887-3, 99(3), 3, 207-215, 2012.03, Analysis of female sex pheromone components and subsequent field trap experiments demonstrated that the bombycid moth Trilocha varians uses a mixture of (E,Z)-10,12-hexadecadienal (bombykal) and (E,Z)-10,12-hexadecadienyl acetate (bombykyl acetate) as a sex pheromone. Both of these components are derivatives of (E,Z)-10,12-hexadecadienol (bombykol), the sex pheromone of the domesticated silkmoth Bombyx mori. This finding prompted us to compare the antennal and behavioral responses of T. varians and B. mori to bombykol, bombykal, and bombykyl acetate in detail. The antennae of T. varians males responded to bombykal and bombykyl acetate but not to bombykol, and males were attracted only when lures contained both bombykal and bombykyl acetate. In contrast, the antennae of B. mori males responded to all the three components. Behavioral analysis showed that B. mori males responded to neither bombykal nor bombykyl acetate. Meanwhile, the wing fluttering response of B. mori males to bombykol was strongly inhibited by bombykal and bombykyl acetate, thereby indicating that bombykal and bombykyl acetate act as behavioral antagonists for B. mori males. T. varians would serve as a reference species for B. mori in future investigations into the molecular mechanisms underlying the evolution of sex pheromone communication systems in bombycid moths.. |
| 30. | Takaaki Daimon, Masaya Yago, Yu Feng Hsu, Tsuguru Fujii, Yumiko Nakajima, Ryuhei Kokusho, Hiroaki Abe, Susumu Katsuma, Toru Shimada, Molecular phylogeny, laboratory rearing, and karyotype of the bombycid moth, trilocha varians, Journal of Insect Science, 10.1673/031.012.4901, 12, 2012.09, [URL], This study describes the molecular phylogeny, laboratory rearing, and karyotype of a bombycid moth, Trilocha varians (F. Walker) (Lepidoptera: Bombycidae), which feeds on leaves of Ficus spp. (Rosales: Moraceae). The larvae of this species were collected in Taipei city, Taiwan, and the Ryukyu Archipelago (Ishigaki and Okinawa Islands, Japan). Molecular phylogenetic analyses revealed that T. varians belongs to the subfamily Bombycinae, thus showing a close relationship to the domesticated silkworm Bombyx mori (L.), a lepidopteran model insect. A laboratory method was developed for rearing T. varians and the time required for development from the embryo to adult was determined. From oviposition to adult emergence, the developmental zero was 10.47°C and total effective temperature was 531.2 daydegrees, i.e., approximately 30 days for one generation when reared at 28°C. The haploid of T. varians consisted of n = 26 chromosomes. In highly polyploid somatic nuclei, females showed a large heterochromatin body, indicating that the sex chromosome system in T. varians is WZ/ZZ (female/male). The results of the present study should facilitate the utilization of T. varians as a reference species for B. mori, thereby leading to a greater understanding of the ecology and evolution of bombycid moths.. |
| 31. | 山田香織, 川西祐一, 奥間政一郎, 岩崎華子, 山田明徳, 中島 裕美子, 前川 秀彰, 南西諸島に棲息する昆虫・クモ類における転移因子マリナー様配列（MLE: mariner-like element）の比較系統解析 , Entomotech, 35, 13-19, 2011.02. |
| 32. | 奥間政一郎, 川西祐一, 佐々木健志, 日高道雄, 前川秀彰, 中島 裕美子, 亜熱帯に生息する昆虫・クモ類に見出される転移因子マリナー様配列の水平伝播機構の解析 , Entomotech, 34, 31-35, 2010.02. |
| 33. | 鎌内 悠, 川西祐一, 矢後勝也, 小田切顕一, 上田恭一郎, 伴野 豊, 前川 秀彰, Yu-Feng Hsu, 黄勇平, 日高道雄, 中島 裕美子, 台湾産クワコからのマリナー様転移因子の単離とその系統解析, Entomotech, 33, 31-35, 2009.02. |
| 34. | Yuichi Kawanishi, Reiko Takaishi, Miki Morimoto, Yutaka Banno, Si Kab Nho, Hideaki Maekawa, Yumiko Nakajima, A novel maT-type transposable element, BmamaT1, in Bombyx mandarina, homologous to the B. mori mariner-like element Bmmar6, Journal of Insect Biotechnology and Sericology, 77, 1, 45-52, 2008.02, A complex type marner-like element (MLE) was isolated by PCR with genomic DNA of Bombyx mandarina. The clones of this MLE, homologous to the 'Cecropia-ITR-MLE' in BmTNML locus that we have isolated previously from B. mori, were roughly classified into four groups according to the state of inserted elements: (1) Containing a retrotransposon (L1Bm) and another element, (2) containing L1Bm, alone, (3) containing another element alone and (4) without insertions. This inserted element was named BmamaT1, as it was homologous to the B. mori MLE called Bmmar6, which was previously reported to be similar to a maT-family member, Bmmar1. Many of the current Cecropia-ITR-MLE clones had, as the BmamaT1 insertion site, the sequence of TA/TATA, which may be a transposition target site. Moreover, the BmamaT1 elements that inserted into the Cecropia-ITR-MLE region were highly homogeneous, making one group when a phylogenetic tree was made together with BmamaT1 members isolated directly from the B. mandarina genome. Many of the inserted type BmamaT1 elements had complete ORF which: possessed the so-called DDD catalytic triad. All these findings were taken to indicate that a large fraction of the BmamaT1 elements are still highly active.. |
| 35. | Yuichi Kawanishi, Yutaka Banno, Hirofumi Fujimoto, Si Kab Nho, Zhenli Tu, Kazuei Mita, Kozo Tsuchida, Naoko Takada, Hideaki Maekawa, Yumiko Nakajima, Method for rapid distinction of Bombyx mandarina (Japan) from B. mandarina (China) based on rDNA sequence differences, Journal of Insect Biotechnology and Sericology, 77, 2, 79-85, 2008.06, The EcoRI site is located between the 5.8S and 28S rRNA genes in Bombyx mandarina (Japan) (Japanese type rDNA) but is absent in the rDNA of B. mandarina (China)(B. mori type rDNA) including the domesticated B. mori. Digestion of PCR products amplified by primers of both flanking sides of this EcoRI site with EcoRI should only be successful for amplification products of B. mandarina (Japan) containing Japanese type rDNA. The distribution of Japanese type rDNA in Japan, Korea, and China was determined using this rapid detection method. Japanese type was not detected in China or in Korea despite having 27 chromosomes. These findings suggest that the choromosome fusion and the rDNA type separation were independent evolutionary events. Invasion of B. mori chromosome #11 into Japanese B. mandarina (Japan) was examined.. |
| 36. | Tomonori Kikuchi, Satoshi Miyazaki, Hitoshi Ohnishi, Junichi Takahashi, Yumiko Nakajima, Kazuki Tsuji, Small queens and big-headed workers in a monomorphic ponerine ant, Die Naturwissenschaften, 10.1007/s00114-008-0414-8, 95, 10, 963-968, 2008.10, [URL], Evolution of caste is a central issue in the biology of social insects. Comparative studies on their morphology so far suggest the following three patterns: (1) a positive correlation between queen-worker size dimorphism and the divergence in reproductive ability between castes, (2) a negative correlation among workers between morphological diversity and reproductive ability, and (3) a positive correlation between queen-worker body shape difference and the diversity in worker morphology. We conducted morphological comparisons between castes in Pachycondyla luteipes, workers of which are monomorphic and lack their reproductive ability. Although the size distribution broadly overlapped, mean head width, head length, and scape length were significantly different between queens and workers. Conversely, in eye length, petiole width, and Weber's length, the size differences were reversed. The allometries (head length/head width, scape length/head width, and Weber's length/head width) were also significantly different between queens and workers. Morphological examinations showed that the body shape was different between queens and workers, and the head part of workers was disproportionately larger than that of queens. This pattern of queen-worker dimorphism is novel in ants with monomorphic workers and a clear exception to the last pattern. This study suggests that it is possible that the loss of individual-level selection, the lack of reproductive ability, influences morphological modification in ants.. |
| 37. | 川西祐一, 伴野 豊, 藤本浩文, 中島 裕美子, 前川秀彰, カイコとクワコの進化的繋がり＝転移因子研究との関わりを含めて , Entomotech , 32, 79-86, 2008.02. |
| 38. | Man Lee, Masateru Takahashi, Hiroaki Mon, Hitoshi Mitsunobu, Katsumi Koga, Yutaka Kawaguchi, Yumiko Nakajima, Takahiro Kusakabe, Construction of gene expression systems in insect cell lines using promoters from the silkworm, Bombyx mori, Journal of Biotechnology, 10.1016/j.jbiotec.2007.08.033, 133, 1, 9-17, 2008.01, [URL], The promoter regions of the Bombyx mori HSC70-4 and B. mori TCTP genes characterized previously were used for the construction of a series of constitutive gene expression systems active in cultured cells. The relative abilities of these promoters were evaluated by comparing those of a silkworm actin A3 (BmActin3) promoter, which is used widely as the first choice. A series of constitutive expression systems constructed were assayed for the transcription efficiency by connecting four reporter cDNAs, firefly luciferase, 3GFP, Ds-Red, and β-galactosidase gene using the Gateway LR reaction. The insertion of an intron enhancer into the site between the TCTP promoter and gene increased the transcription of the BmTCTP promoter by 10-fold. The insertion of the IE-1 gene and HR3 enhancer to the all three promoters were found to increase the transcription up to 560 times.. |
| 39. | Farhana Naznin, Morikazu Nakamura, Takeo Okazaki, Yumiko Nakajima, Two Phase Evolutionary Method for Multiple Sequence Alignments, Proceeding Paper of the First International Symposium on Optimization and System Biology (OSB’07) Beijig, China, Augst 8-10, ORSC APORC. , 309-323, 2007.08, This paper presents a new evolutionary method, namely, a Two Phase evolutionary algo- rithm for multiple sequence alignments. This method is composed of different types of evolutionary algorithms, that is, an evolutionary progressive multiple sequence alignment method (abbreviated to ET) and Sequence Alignment by Genetic Algorithm (abbreviated to SAGA). The former is em- ployed to obtain efficiently good quality of multiple alignments and the latter to improve them much better. The basic idea was obtained from analysis of the characteristics of the two evolution- ary methods, that is, the ET and SAGA can compensate each other’s weak points. Experimental evaluation shows that the proposed Two Phase method can generate good quality of multiple align- ments.. |
| 40. | Farhana Naznin, Morikazu Nakamura, Takeo Okazaki, Yumiko Nakajima, An evolutionary progressive multiple sequence alignment, 2007 IEEE Congress on Evolutionary Computation, CEC 2007 2007 IEEE Congress on Evolutionary Computation, CEC 2007, 10.1109/CEC.2007.4424977, 3886-3893, 2007.08, [URL], This paper proposes an evolutionary tree-base (progressive multiple sequence alignment) method using a genetic algorithm (GA) for solving multiple sequence alignment problems. In our evolutionary tree-base method, chromosomes are represented as guide trees. Two kinds of crossover are proposed for chromosomes of tree structure; subtree selection crossover and tree uniform order crossover. They can generate new chromosomes with inheriting tree structure of parents. The indirect representation of multiple alignments, namely, the guide tree representation of chromosomes, and the proper genetic operations make searching drastically efficient. Experimental results for benchmark problems from BAIiBASE and the NCBI database show that the proposed method is superior to SAGA (a well-known GA-base approach, 1996), T-Coffee (sensitive progressive method, 2000), MUSCLE (progressive/iterative method, 2004), MAFFT (progressive/iterative method, 2005), and ProbCons (probabilistic/consistency method, 2005) with regard to quality of solutions.. |
| 41. | Naoko Takada, Emiko Yamauchi, Hirofumi Fujimoto, Yutaka Banno, Kozo Tsuchida, Kazuo Hashido, Yumiko Nakajima, Zhenli Tu, Masateru Takahashi, Hiroshi Fujii, Hajime Fugo, Hideaki Maekawa, A novel indicator for radiation sensitivity using the wing size reduction of Bombyx mori pupae caused by γ-ray irradiation, Journal of Insect Biotechnology and Sericology, 75, 3, 161-165, 2006.10, The wings of Bombyx mori are known to become smaller when irradiated with γ-rays at the larval stage. This was considered to be a non-stochastic effect wherein the wing-size reduction curve, plotted vs. the irradiation dose, shows a threshold. Here we propose a new indicator for radiation sensitivity, named the wingless dose 50 (WLD50), which was obtained from the inflection point of wing-size data normalized by the body-size changes and plotted against the irradiation dose. This indicator was confirmed to serve as a tool to compare irradiation sensitivity among B. mori strains.. |
| 42. | Yuichi Kawanishi, Reiko Takaishi, Yutaka Banno, Hirofumi Fujimoto, Si Kab Nho, Hideaki Maekawa, Yumiko Nakajima, Sequence comparison of mariner-like elements among the populations of Bombyx mandarina inhabiting China, Korea and Japan, Journal of Insect Biotechnology and Sericology, 76, 2, 79-87, 2007.06, Mariner-like elements (MLEs) were isolated from Chinese, Korean and Japanese individuals of Bombyx mandarina by the long PCR methods using the inverted terminal repeat of the Hyalophora cecropia MLE as a primer. The amplified clones of about 1.3 kbp in size were regarded to be full-length MLEs and termed Cecropia-ITR-MLEs. The sequences of the elements were determined, trimmed to eliminate any insertions and used to construct a phylogenetic tree. As a result, the Cecropia-ITR-MLE sequences of B. mandarina could be divided into major groups here called the Continental type and the Japanese type. Chinese and Korean specimens mostly belonged to the former group, which is very diverse and may well consist of subgroups. A majority of Honshu (the main island of Japan) specimens belonged to the latter, which is a much more closely related group of sequences. Interestingly, Tsushima (south of Korea) and Hokkaido (close to Russia) specimens mainly fell into the former type, whereas Fukuoka (south of Tsushima) individuals included both.. |
| 43. | Yutaka Kawaguchi, Takahiro Kusakabe, Man Lee, Yumiko Nakajima, Katsumi Koga, Micropylar structure of chorion of the female sterile mutation, bd, in Bombyx mori, Journal of Insect Biotechnology and Sericology, 75, 1, 9-14, 2006.02, Micropylar structures of chorion in the bd mutant group of Bombyx mori were observed under a scanning electron microscope, with special reference to the external features of the micropylar rosette, orifice and internal channel tubes. These structures were normal in the control eggs, i.e., heterozygous for bd, bdsw and bdf alleles, but were deformed in the bd and bdsw homozygous eggs, both of which were sterile. In particular, the channel tubes were not present in these eggs. On the other hand, the micropylar architecture of the bdf homozygous eggs, which were fertile, was normal like control eggs. Based on these results, we suggest that the sterility of the bd and bdsw females is caused by the abnormality of the micropylar apparatus, which may be crucial for fertilization processes.. |
| 44. | Kazuyo Kurose, Masanori Terai, Nurtami Soedarsono, Doralina Rabello, Yumiko Nakajima, Robert D. Burk, Minoru Takagi,, Low prevalence HPV infection and its natural history in normal oral mucosa among volunteers on Miyako Island, Japan. , Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 10.1016/j.tripleo.2003.12.029, 98, 1, 91-96, 2004.07, Objective. To investigate the prevalence of human papillomavirus (HPV) infection in normal oral mucosa, and to observe the natural history in the oral cavity in oral swab samples collected from healthy volunteers on Miyako Island, Okinawa, Japan. Study design. The prevalence of HPV infection in oral buccal mucosa cell scrapes collected between 2000 and 2002 from a cohort of 668 healthy volunteers was determined. HPV DNA was detected by consensus polymerase chain reaction (PCR) using MY09/MY11 primers followed by direct cycle sequencing. Just over 2 years later the HPV-positive participants were reevaluated. Results. Of the 668 subjects, 662 samples were analyzed for HPV. HPV DNA was detected in 4 (0.6%) specimens. HPV type 16 (HPV16), HPV53, and HPV71, mucosal types, and HPV12, a cutaneous type, were all identified by direct sequencing. In the follow-up survey, the HPV71- and HPV12-positive participants again tested positive, while HPV DNA was not detected in the HPV16- and HPV53-positive participants. Conclusion. The results of this study among healthy individuals from Miyako Island suggest that oral HPV infection is uncommon. In this cohort, HPV71 and HPV12 were persistent, while HPV16 and HPV53 were transient in normal oral mucosa.. |
| 45. | Hiromu Toma, Jun Kobayashi, Yumi Imada, Takeshi Arakawa, Yumiko Nakajima, Sakhon Laymanivong, Bouakham Vannachone, Khemphabanh Manivong, Samlane Phompida, Yoshiya Sato, Field application and evaluation of a rapid immunochromatographic test for detection of Plasmodium falciparum infection among the inhabitants of Lao PDR, Southeast Asian Journal of Tropical Medicine and Public Health, 34, 1, 43-47, 2003.03, Field application and evaluation of a rapid immunochromatographic test (ICT) for detection of Plasmodium falciparum infection were performed in 13 villages in a southern province of Lao PDR in 1999. More than 2,000 inhabitants, accounting for 61.8% of the total estimated population, were examined. Malaria infection was confirmed in all villages surveyed by ICT and microscopic diagnosis. The positive rates of P. falciparum malaria by microscopy ranged from 9.7% to 59.2% (mean 27.2%), whereas by ICT they were from 11.6% to 64.5% (mean 29.8%). The positive rates by ICT were generally higher in 8 out of 13 villages. However, a significant difference between the positive rates by microscopy and ICT was not observed in all villages. Plasmodium falciparum infection was actually confirmed by microscopy in 84.1% of specimens that tested positive by ICT. The results by ICT were consistent with those of the microscopic diagnosis, the discrepancy of the results was less than 10% (141/2,066). The ICT was falsely-positive in 4.7% and falsely-negative in 2.1% of the test cases. These results showed the efficacy of ICT not only in the diagnosis of the respective cases, but also in the mass-examination in the field.. |
| 46. | Man Lee, Takahiro Kusakabe, Yutaka Kawaguchi, Chisa Yasunaga-Aoki, Si Kab Nho, Yumiko Nakajima, Katsumi Koga, Molecular characterization of a heat shock cognate 70-4 promoter from the silkworm, Bombyx mori, Journal of Insect Biotechnology and Sericology, 72, 1, 33-39, 2003.02, A cDNA stretch encoding one of the silkworm HSC70 genes with its upstream sequence has been identified, based on partial cDNA sequences registered in a Bombyx mori EST database. The deduced amino acid sequence with 649 residues was 89 and 96% identical to those from Drosophila melanogaster hsc4 and Manduca sexta Hsc70-4, respectively. The expression analysis by the reverse transcription polymerase chain reaction demonstrated that the mRNA transcription occurred in all tissues examined and was not stimulated by heat shock. These results suggested that the current structure corresponds to the silkworm heat shock cognate 70-4 gene and named BmHSC70-4. Cloning and sequencing of the genomic 5'-flanking, putative promoter region of BmHSC70-4 revealed the presence of several canonical transcription elements such as the GATA box, CCAAT motif and HSE (heat shock element), although without a TATA box element. The deletion analysis of the 5' flanking region of the BmHSC70-4 gene, that fused to the firefly luciferase coding sequence as a reporter, indicated that the longest construct (-1,639/+26) showed the highest promoter activity, and the constructs -111 to -59 and -1,110 to -941 contained the basal transcription enhancer and the repressor elements, respectively.. |
| 47. | Atsuko Ota, Takahiro Kusakabe, Yasushi Sugimoto, Masateru Takahashi, Yumiko Nakajima, Yutaka Kawaguchi, Katsumi Koga, Cloning and characterization of testis-specific tektin in Bombyx mori, Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 10.1016/S1096-4959(02)00153-7, 133, 3, 371-382, 2002.11, [URL], A testis-specific cDNA library of Bombyx mori was constructed by an mRNA subtraction technique. Several clones were randomly selected and determined for their nucleotide sequences. One of them, designated as BmTST, contained a 3′-part of an open reading frame homologous to tektin, the protein known to form filamentous polymers in the walls of ciliary and flagellar microtubules. Also isolated was a genomic fragment, which contains the 5′-part of the coding sequence of BmTST and its promoter region. As a whole, the complete open reading frame was found to encode 508 amino acid residues, whose sequence had 28, 28 and 30% identities with the Strongylocentrotus purpuratus tektins A1, B1 and C1, respectively. Expression analysis by reverse transcription polymerase chain reaction with the cDNA and Western blotting with a polyclonal antibody indicated that the BmTST gene was expressed specifically in the testis during sperm maturation. The protein was immunologically detected exclusively in the fraction expected to contain the 9+2 flagellar axonemes of sperms. We infer that the BmTst protein is possibly involved in the spermatogenesis of B. mori.. |
| 48. | Yumiko Nakajima, Takashi Nakamura, Yutaka Banno, Hirofumi Fujimoro, Kazuo Hashido, Teiichiro Shino, Kozo Tsuchida, Naoko Takada, Hideaki Maekawa, Comparison of mariner-like elements among Bombyx mandarina individuals inhabiting East Asia in the light of the segregation of B. mori and B. mandarina genomes, International Journal of Wild Silkmoth & Silk, 8, 57 -64, 2003.01. |
| 49. | Yumiko Nakajima, Hirofumi Fujimoto, Tsutomu Negishi, Kazuo Hashido, Teiichiro Shiino, Kozo Tsuchida, Michio Hidaka, Naoko Takada, Hideaki Maekawa, Possible horizontal transfer of mariner-like sequences into some invertebrates including lepidopteran insects, a grasshopper and a coral, Journal of Insect Biotechnology and Sericology, 71, 3, 109-121, 2002.10, Marine-like elements (MLEs) were amplified by PCR against genomic DNAs from several Japanese lepidopteran species, using the inverted terminal repeats (IR) of the Hyalophora cecropia MLE as a primer. Clones thus obtained were of a size of about 1.3 kb, and expected to contain the full-length MLE. A 1.3-kb band was also amplified against genomic DNA from the grasshopper, Traulia ornata, and from a coral allocated to the Fungia family. All of the 1.3-kb bands were cloned and analyzed for nucleotide sequence. Multi-alignment analyses of the results indicated that the clones were highly similar to each other and classified into the cecropia subfamily of MLEs. The coral MLE was found to have a complete ORF coding for transposase, a situation similar to that previously found in the Emperor moth, Attacus atlas, from the Ryukyu island. These findings, together with the fact that all of the insects and coral species that exhibited a positive signal for the full-length MLE were collected in relatively close regions around Japan, indicated that the horizontal transfer of MLEs had taken place even among phylogenetically remote organisms.. |
| 50. | Bungo Sakaguchi, Marian R. Goldsmith, Nikolaus A. Spoerel, Yumiko Nakajima, Yutaka Kawaguchi, Katsumi Koga, Hybridization diversity of the chorion multigene families of Bombyx mandarina with reference to several genetic stocks of Bombyx mori , Journal of insect Biotechnology and Sericology, , 70, 3, 173-182, 2001.10, Genomic Southern blotting analyses of Bombyx mori and B. mandarina were performed with the 5′-exons or 5′-flanking sequences of two members of genes A/B.L11 and A/B.L12. They represent multigene families encoding chorion proteins that are expressed in the middle period of choriogenesis. Many bands cross-hybridized with the probes in B. mandarina and in different strains and races of B. mori, supporting the idea that the middle chorion multigene families have remained essentially the same in the two species. Both the exon and the 5′-flanking probes exhibited similar patterns within the European stocks of B. mori, indicating that the examined DNA regions have been conserved. Patterns revealed in a Japanese and a Chinese strain showed distinct differences in band number and fragment size, indicating that the chorion gene families are not fixed within the domesticated silkworm. A greater number of fragments hybridized with the exon probes in B. mandarina than in B. mori; this may have relevance to the previous finding that B. mandarina has a large number of proteins with high cysteine content. The banding patterns for both the exon and 5′-flanking probes were markedly different between B. mori and B. mandarina, indicating that major evolutionary differences exist between the two species. . |
| 51. | Takahiro Kusakabe, Yasushi Sugimoto, Takuji Maeda, Yumiko Nakajima, Masaru Miyano, Jun-ichi Nishikawa, Shigenobu Tone, Yutaka Kawaguchi, Katsumi Koga, Takashi Ohyama, Linearization and integration of DNA into cells preferentially occurs at intrinsically curved regions from human LINE-1 repetitive element , Gene, 10.1016/S0378-1119(01)00631-X, 1-2, 274, 271-282, 2001.08, A bent DNA library was constructed from human genomic DNA, from which a new clone belonging to the human LINE-1 sequence family was isolated and characterized. This clone, with a length of 378 base pairs and termed HBC-1 (human bent clone-1), contained an intrinsically occurring curved DNA structure. By permutation analysis, the center of curvature of this fragment was mapped onto the nucleotide position 886 from the 5′ terminus of the complete LINE-1 sequence. Reporter plasmids, which contain HBC-1, were effectively integrated into human chromosome, indicating that the bent DNA structure provides a preferential donor site for the integration of human LINE-1 sequences. The present finding may provide an explanation as to why some inactivated LINE-1 sequences on human chromosomes carry the deletion at their 5′ termini.. |
| 52. | K. Funatsuki, K. Hashido, M. Matsunami, Y. Kameoka, K. Iwabuchi, H. Tsukeda, K. Tsuchida, N. Takada, Yumiko Nakajima, H. Maekawa, Rapid identification of Bombyx mori cells using PCR amplification following a direct procedure for genomic DNA preparation, Journal of Insect Biotechnology and Sericology, 70, 2, 129-136, 2001.02, In cultured cells, karyotypes and genomic dose are variable, especially in ones passaged for a long time. We have improved a method for the genome confirmation of cultured cells of the silkworm, Bombyx mori. A rapid, simple procedure was developed for direct extraction of genomic DNA from cultured cells which has two main steps of proteinase K treatment and heating, without phenol extraction. Genomic characterization is based on PCR amplification of three known genes which vary in copy number and chromosome location. The retrotransposon, BMC1, is dispersed in the genome; rDNA is clustered in one locus; and the fibroin gene is single copy. These genes were sufficient to identify cells as Bombyx mori for comparing among the other species. It was shown that this method could also be used for genotyping and strain identification using larval hemolymph samples.. |
| 53. | Yumiko Nakajima, Kazuo Hashido, Kozo Tsuchida, Naoko Takada, Teiichiro Shiino, Hideaki Maekawa, A novel tripartite structure comprising a mariner-like element and two additional retrotransposons found in the Bombyx mori genome, Journal of Molecular Evolution, 10.1007/PL00006500, 48, 5, 577-585, 1999.05, [URL], A clone of a DNA-mediated mobile element (transposon) corresponding to a mariner-like element (MLE) was obtained by carrying out the polymerase chain reaction with genomic DNA of Bombyx mori using a Hyalophora cecropia MLE sequence as a primer. This clone had a size of about 4.2 kb and, after sequencing, was found to contain an RNA-mediated, shorter retrotransposon named L1Bm, which was in turn integrated with a much longer retrotransposon named BMC1. Thus, the mobile elements made a novel tripartite structure. The BMC1 and L1Bm moieties of the composite structure each contained a 63-bp conserved sequence which was subsequently found to be highly conserved in all BMC1 and L1Bm elements registered so far. We propose that the 63-bp stretch may be a recognition site for a retrotransposition mechanism conducted by a reverse transcriptase and an endonuclease complex. On the basis of this inference, we propose a model that predicts how different types of BMC1 and L1Bm elements are dispersed in the genome. In addition, a phylogenetic tree made from the current and extant BMC1 and L1Bm sequences indicated that these elements can be classified into Subfamilies I and II.. |
| 54. | Yumiko Nakajima, Masaru Nagamine, Kazuo Hashido, Kozo Tsuchida, Teiichiro Shiino, Toshihiko Hayashi, Hldeaki Maekawa, Isolation of a mariner-like sequence containing a complete open reading frame for transposase from Attacus atlas and its phylogenetic relationships within the Ditrysia of Lepidoptera, Journal of Insect Biotechnology and Sericology, 10.11416/kontyushigen1930.67.271, 67, 4, 271-278, 1998.07, [URL], A complete open reading frame (ORF) encoding a transposase was found in the mariner-like element of the Emperor moth (Yonaguni-san), Attacus atlas. The transposase sequence was isolated by PCR amplification using primers derived from the inverted terminal repeat of Hyalophora cecropia. Within the ORF of the transposase sequence, two consensus sequences and a D, D (34) D motif were found. The sequence of A. atlas was homologous to mariner-like elements from Bombyx mori, H. cecropia, Manduca sexta, Sitotroga cerealella and Ephestia cautella. These results suggested that the mariner-like elements and the inverted terminal repeat may be conserved among Bombycoidea and related species of Ditrysia. Bmmarl, previously reported to be an element from B. mori, showed very low homology compared to sequences from Lepidoptera and other insect groups.. |
| 55. | Tsuyoshi Asato, Yumiko Nakajima, Masaru Nagamine, Yasutsugu Nakashima, Hiroshi Takei, Toshiyuki Maehama, Takenobu Yamashiro, Masahiro Higashi, Michio Nakayama, Koji Kanazawa, Correlation between the Progression of Cervical Dysplasia and the Prevalence of Human papillomavirus, The Journal of Infectious Diseases, 10.1093/infdis/169.4.940, 169, 4, 940 -941, 1994.04, . |
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