Kyushu University Academic Staff Educational and Research Activities Database
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Matsuda Taito Last modified date:2019.06.25





E-Mail
Phone
092-642-6196
Fax
092-642-6561
Academic Degree
Doctor of Medicine
Country of degree conferring institution (Overseas)
No
Field of Specialization
Neuroscience
Total Priod of education and research career in the foreign country
00years00months
Research
Research Interests
  • Direct reprogramming of microglia into neurons
    keyword : direct reprogramming
    2018.04~2021.05.
  • Identify the factors inducing the impairment of neural stem cell proliferation during aging
    keyword : Neural stem cell
    2018.04~2022.05.
Academic Activities
Papers
1. Matsuda T * (co-corresponding author)., Irie T., Katsurabayashi S., Hayashi Y., Nagai T., Hamazaki N., Adefuin AMD., Miura F., Ito T., Kimura H., Shirahige K., Takeda T., Iwasaki K., Imamura T. & Nakashima K*., Pioneer Factor NeuroD1 Rearranges Transcriptional and Epigenetic Profiles to Execute Microglia-Neuron Conversion, Neuron, 101, 472-485, 2019.01, Minimal sets of transcription factors can directlyreprogram somatic cells into neurons. However,epigenetic remodeling during neuronal reprogram-ming has not been well reconciled with transcrip-tional regulation. Here we show that NeuroD1achieves direct neuronal conversion from mouse mi-croglia bothin vitroandin vivo. Exogenous NeuroD1initially occupies closed chromatin regions associ-ated with bivalent trimethylation of histone H3 atlysine 4 (H3K4me3) and H3K27me3 marks in micro-glia to induce neuronal gene expression. These re-gions are resolved to a monovalent H3K4me3 markat later stages of reprogramming to establish theneuronal identity. Furthermore, the transcriptionalrepressorsScrt1andMeis2are induced as NeuroD1target genes, resulting in a decrease in the expres-sion of microglial genes. In parallel, the microglialepigenetic signature in promoter and enhancerregions is erased. These findings reveal NeuroD1pioneering activity accompanied by global epige-netic remodeling for two sequential events: onset ofneuronal property acquisition and loss of the micro-glial identity during reprogramming..
Membership in Academic Society
  • The Japane Neuroscience Sciety
  • The Japanese society for Epigenetics