Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Douglas Robert Drummond Last modified date:2019.06.10

Professor / Attached Promotive Center for International Education and Research of Agriculture / Faculty of Agriculture

1. von Löffelholz, O Pena, A Drummond, DR Cross, R Moores, CA, Cryo-EM Structure (4.5-angstrom) of Yeast Kinesin-5-Microtubule Complex Reveals a Distinct Binding Footprint and Mechanism of Drug Resistance, JOURNAL OF MOLECULAR BIOLOGY, 10.1016/j.jmb.2019.01.011, 431, 4, 864-872, 2019.02.
2. von Loeffelholz, Ottilie; Venables, Neil A.; Drummond, Douglas Robert; Katsuki, Miho; Cross, Robert; Moores, Carolyn A., Nucleotide- and Mal3-dependent changes in fission yeast microtubules suggest a structural plasticity view of dynamics, NATURE COMMUNICATIONS, 10.1038/s41467-017-02241-5, 8, 2110, 1-13, 2017.12, [URL], Using cryo-electron microscopy, we characterize the architecture of microtubules assembled from Schizosaccharomyces pombe tubulin, in the presence and absence of their regulatory partner Mal3. Cryo-electron tomography reveals that microtubules assembled from S. pombe tubulin have predominantly B-lattice interprotofilament contacts, with protofilaments skewed around the microtubule axis. Copolymerization with Mal3 favors 13 protofilament microtubules with reduced protofilament skew, indicating that Mal3 adjusts interprotofilament interfaces. A 4.6-Å resolution structure of microtubule-bound Mal3 shows that Mal3 makes a distinctive footprint on the S. pombe microtubule lattice and that unlike mammalian microtubules, S. pombe microtubules do not show the longitudinal lattice compaction associated with EB protein binding and GTP hydrolysis. Our results firmly support a structural plasticity view of microtubule dynamics in which microtubule lattice conformation is sensitive to a variety of effectors and differently so for different tubulins..
3. Frauke Hussman, Douglas R Drummond, Daniel R Peet, Douglas S Martin, Robert A Cross, Alp7/TACC-Alp14/TOG generates long-lived, fast-growing MTs by an unconventional mechanism, SCIENTIFIC REPORTS, 10.1038/srep20653, 6, 2016.02.
4. Miho Katsuki, Douglas R Drummond, Robert A Cross, Ectopic A-lattice seams destabilize microtubules, NATURE COMMUNICATIONS, 10.1038/ncomms4094, 5, 2014.01.
5. Muriel Erent, Douglas R Drummond, Robert A Cross, S. pombe Kinesins-8 Promote Both Nucleation and Catastrophe of Microtubules, PLOS ONE, 10.1371/journal.pone.0030738, 7, 2, 2012.02.
6. Douglas R Drummond, Susan Kain, Anthony Newcombe, Christina Hoey, Miho Katsuki, Robert A Cross, Purification of tubulin from the fission yeast Schizosaccharomyces pombe, Methods in Molecular Biology, 10.1007/978-1-61779-252-6_3, 777, 29-55, 2011.06, [URL].
7. Miho Katsuki, Douglas R Drummond, Michael Osei, Robert A Cross, Mal3 Masks Catastrophe Events in Schizosaccharomyces pombe Microtubules by Inhibiting Shrinkage and Promoting Rescue, JOURNAL OF BIOLOGICAL CHEMISTRY, 10.1074/jbc.C109.052159, 284, 43, 29246-29250, 2009.10.
8. Marcus Braun, Douglas R Drummond, Robert A Cross, Andrew D McAinsh, The kinesin-14 Klp2 organizes microtubules into parallel bundles by an ATP-dependent sorting mechanism, NATURE CELL BIOLOGY, 10.1038/ncb1878, 11, 6, 724-U55, 2009.06.
9. Amedee des Georges, Miho Katsuki, Douglas R Drummond, Michael Osei, Robert A Cross, Linda Amos, Mal3, the Schizosaccharomyces pombe homolog of EB1, changes the microtubule lattice, NATURE STRUCTURAL & MOLECULAR BIOLOGY, 10.1038/nsmb.1482, 15, 10, 1102-1108, 2008.10.
10. Maria C Alonso, Douglas R Drummond, Susan Kain, Julia Hoeng, Linda Amos, Robert A Cross, An ATP gate controls tubulin binding by the tethered head of kinesin-1, SCIENCE, 10.1126/science.1136985, 316, 5821, 120-123, 2007.04.
11. Douglas R Drummond, Nick Carter, Robert A Cross, Multiphoton versus confocal high resolution z-sectioning of enhanced green fluorescent microtubules: increased multiphoton photobleaching within the focal plane can be compensated using a Pockels cell and dual widefield detectors, JOURNAL OF MICROSCOPY-OXFORD, 206, 161-169, 2002.05.
12. Douglas R Drummond, Robert A Cross, Dynamics of interphase microtubules in Schizosaccharomyces pombe, CURRENT BIOLOGY, 10, 13, 766-775, 2000.06.