Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Yoko Yamanishi Last modified date:2018.06.20

Professor / 流体医工学 / Department of Mechanical Engineering / Faculty of Engineering


Papers
1. S. Takasawa, S. Hosoda, Yoko Yamanishi, Investigation of molecular condensation on air-liquid interface for protein crystallization, 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015, 10.1109/TRANSDUCERS.2015.7180955, 440-443, 2015.08, This paper investigated the mechanism of the condensation of protein molecules on air-liquid interface of bubbles. Electrically charged bubbles were confirmed to have clear advantage of producing protein crystal over general bubbles or conventional vapor diffusion method for the first time. The adsorption force between the protein molecular and air-liquid interface of bubble was successfully measured using force sensing optical tweezers. These findings contribute to the effective production of protein crystal and to the protein crystallography..
2. Kazuki Takahashi, Yuta Arakawa, Yoko Yamanishi, High dynamic ablation and injection by electric cavitation for wide range of materials, 29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016 MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems, 10.1109/MEMSYS.2016.7421547, 2016-February, 21-24, 2016.02, We have successfully carried out local reagent injection and perforation to materials of variety of hardness using bubble cavitation and plasma cavitation. Cavitation and plasma was generated by pulse discharge of microelectrode having special tip structure. Injection to soft material such as animal cell was performed only by cavitation of bubble, and perforation to hard materials such as seeds of plants or metals were achieved by synergistic effect of cavitation of bubble and plasma ablation. The novel device we produced has ability to perforate wide range of materials with high dynamic range of hardness..
3. Yohei Hamano, Takuya Kambayashi, Yoko Yamanishi, Multiple-reagent layer laden high-speed micro-bubbles in extended two-dimensional microchip, International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015 2015 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2015, 10.1109/MHS.2015.7438236, 2016.03, In conventional chemical analysis methods, cohesion and ununiformed concentration within the mixed solution tend to be occurred during the reagent mixing process, which causes the increase of reagent consumption and process time. In the past, the authors have invented a device called bubble injection knife. In this device, high-speed micro-bubbles are ejected piercing through a reagent interface by discharging from a Cu wire, transporting a thin reagent membrane formed on the surface of the bubble due to its adsorption force. In addition, it is known that micro-bubbles contract and collapse. In this study, the authors designed a device using the properties of micro-bubbles and the bubble injection knife, which purpose is to reduce reagent consumption and enable uniform mixing in chemical analysis. This device is a micro-chip which is designed to produce micro-bubbles by electric discharge from a bubble kife embedded in the micro-channel and make them penetrate through reagent laminar flows in order to form reagent layers on the bubble's surface. Moreover, the mixing of these layers using the contraction force of the bubbles is expected. In the experiments that were conducted, micro-bubbles were produced in the micro-channel from the embedded bubble knife. This technology is expected to improve chemical analysis methods to overcome ununiformed concentration within the mixed solution, and also it is expected to contribute to the bio-medical reagent injection with control of reaction time..
4. Naoyuki Kurake, Hiromasa Tanaka, Kenji Ishikawa, Kae Nakamura, Hiroaki Kajiyama, Fumitaka Kikkawa, Masaaki Mizuno, Yoko Yamanishi, Masaru Hori, Synthesis of calcium oxalate crystals in culture medium irradiated with non-equilibrium atmospheric-pressure plasma, Applied Physics Express, 10.7567/APEX.9.096201, 9, 9, 2016.09, Octahedral particulates several tens of microns in size were synthesized in a culture medium irradiated through contact with a plume of nonequilibrium atmospheric-pressure plasma (NEAPP). The particulates were identified in the crystalline phase as calcium oxalate dihydrate (COD). The original medium contained constituents such as NaCl, D-glucose, CaCl2, and NaHCO3 but not oxalate or oxalic acid. The oxalate was clearly synthesized and crystallized in the medium as thermodynamically unstable COD crystals after the NEAPP irradiation..
5. Sohei Itabashi, Takuya Kambayashi, Takatoshi Shimamura, Kazuki Takahashi, Yoko Yamanishi, In-situ cellular-scale injection for alive plants by micro-bubble injector, 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016, 10.1109/NEMS.2016.7758324, 604-607, 2016.11, We have succeeded in local and in-situ injection of reagent into a single plant cell by using the cavitation phenomenon of micro-bubbles. Micro-bubbles were generated electrically by a novel device called 'micro-bubble injector'. This novel technology provides minimally invasive processing and high accessibility compared to conventional injection techniques which enable to inject to the material which have been difficult to be injected for a long time. This technology contribute to the biological and medical researches developments..
6. Keishi Ohtonari, Yuta Arakawa, Hiroki Ogata, Daisuke Tujimoto, Yoko Yamanishi, High-throughput injection by circulating plasma-bubbles laden flows, 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016, 10.1109/NEMS.2016.7758218, 144-147, 2016.11, We have succeeded in injection of plasmid to adherent cells which are suspended in the plasma-bubbles laden circulation flow in a chamber. High-speed plasma-bubbles are generated by glass electrode and the air-liquid interface has a stiction force which draws the gene (plasmid) and stick to the air-liquid interface. The circulating flow increased the chances for cells to contact air-liquid interface of bubbles which is enclosing plasma or reactive gas. Finally, the high reactive interface enables gene transfer to cells efficiently. This proposed two dimensional microfluidic chip contributes to high-throughput gene transfer in bio-medical applications..
7. Tasuku Sato, Yoko Yamanishi, Vito Cacucciolo, Yu Kuwajima, Hiroki Shigemune, Matteo Cianchetti, Cecilia Laschi, Shingo Maeda, Electrohydrodynamic conduction pump with asymmetrical electrode structures in the microchannels, Chemistry Letters, 10.1246/cl.170217, 46, 7, 950-952, 2017, In this study, we have developed a microfluidic pump that utilizes the electrohydrodynamic (EHD) conduction of a working fluid. Ring-shaped pumps have been used in previous studies on EHD conduction, but these require a three-dimensional arrangement of electrode pairs, which makes it difficult to downsize the apparatus. Here, we propose a mechanism to achieve one-way fluid flow in the microchannels by arranging non-parallel electrodes in a plane to generate an asymmetric electric field. One advantage of this design is that it can be easily and precisely fabricated using microelectromechanical system (MEMS) processing techniques: This has allowed us to integrate a micropump and a microchannel into a single device. Moreover, the pressure generated by the pump is induced solely by electrochemical reactions; since mechanical components such as gears are not required, this helps reduce the noise generated by the device..
8. Yoko Yamanishi, Ryotaro Tanaka, Yuta Arakawa, Yoshimichi Nakatsu, Gene transfer by circulating plasma bubble flow, 30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017, 10.1109/MEMSYS.2017.7863438, 444-447, 2017.02, We have succeeded in injection of plasmid to adherent cells which are suspended in the plasma-bubbles laden circulation flow in a chamber. High-speed plasma-bubbles are generated by glass electrode and the air-liquid interface has a stiction force which draws the gene (plasmid) and stick to the air-liquid interface. The circulating flow increased the chance for cells to contact air-liquid interface of bubbles which enclosed plasma or reactive gas. Finally, the high reactive interface enables gene transfer to cells efficiently. This technology of two-dimensional microfluidic chip contributes an option to the high-throughput gene transfer..
9. Yoko Yamanishi, Daisuke Matsumura, Yuya Fujiwara, Takashi Ohgawara, Yoshikazu Haramoto, Local magnetization and sensing of flexible magnetic tag for long-term monitoring under wet environment, 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems, 10.1109/TRANSDUCERS.2017.7994086, 460-463, 2017.07, We have successfully fabricated and installed flexible magnetic tag which has about 9 bits information to biological material in limited space under wet environment. The magnetizing of tag use focusing of magnetic field based on the magnetic circuit using high magnetic permeability material. Fundamental test for the magnetic tag show the magnetic signal can be discriminated even 30 days after injection of magnetic tag. This study can contribute to identify the various gene transferred biomaterials and contribute to the gene technology and long-term monitoring of environment..
10. Keita Ichikawa, Shingo Maeda, Yoko Yamanishi, Evaluation of Invasiveness by Breakdown Phenomena of Electrically Induced Bubbles for a Needle-Free Injector, Journal of Microelectromechanical Systems, 10.1109/JMEMS.2018.2793314, 27, 2, 305-311, 2018.03, This paper evaluates a needle-free injector using electrically induced bubbles. For a minimally invasive injector, the configuration of the bubble reservoir, which is at the tip space of the bubble injector, must be optimized because the invasiveness depends on the breakdown distance of the electrically induced bubble..
11. Tasuku Sato, Yoko Yamanishi, Vito Cacucciolo, Yu Kuwajima, Hiroki Shigemune, Matteo Cianchetti, Cecillia Laschi, Shingo Maeda, Electrohydrodynamic Conduction Pump with Asymmetrical Electrode Structures in the Micro-channels, Chemistry Letters, doi:10.1246/cl.170217, doi:10.1246/cl.170217, 2017.04, [URL], In this study, we develop a microfluidic pump which utilizes the electrohydrodynamic (EHD) conduction of a working fluid. Ring-shaped pumps have been used in previous studies on EHD conduction, but these require a three-dimensional arrangement of electrode pairs, which makes it difficult to downsize the apparatus. Here, we propose a mechanism to achieve one-way fluid flow in the micro-channels by arranging non-parallel electrodes in a plane to generate an asymmetric electric field. One advantage of this design is that it can be easily and precisely fabricated using microelectromechanical systems (MEMS) processing techniques: this has allowed us to integrate a micro-pump and a micro-channel into a single device. Moreover, the pressure generated by the pump is induced solely by electrochemical reactions; as mechanical components such as gears are not required, this helps to reduce noise generated by the device..
12. Naoyuki Kurake, Hiromasa Tanaka, Kenji Ishikawa, Kae Nakamura, Hiroaki Kajiyama, Fumitaka Kikkawa, Masaaki Mizuno, Yoko Yamanishi, Masaru Hori, Synthesis of calcium oxalate crystals in culture medium irradiated with non-equilibrium atmospheric-pressure plasma, Applied Physics Express 9, 096201 (2016), 9, 096201, 2016.07.