九州大学-研究者情報 [松沢健司 (講師) 理学研究院生物科学部門]

Kenji Matsuzawa, Hayato Ohga, Kenta Shigetomi, Tomohiro Shiiya, Masanori Hirashima, Junichi Ikenouchi, MAGIs regulate aPKC to enable balanced distribution of intercellular tension for epithelial sheet homeostasis., Communications Biology, 10.1038/s42003-021-01874-z, 4, 1, 337-337, 2021.03, Constriction of the apical plasma membrane is a hallmark of epithelial cells that underlies cell shape changes in tissue morphogenesis and maintenance of tissue integrity in homeostasis. Contractile force is exerted by a cortical actomyosin network that is anchored to the plasma membrane by the apical junctional complexes (AJC). In this study, we present evidence that MAGI proteins, structural components of AJC whose function remained unclear, regulate apical constriction of epithelial cells through the Par polarity proteins. We reveal that MAGIs are required to uniformly distribute Partitioning defective-3 (Par-3) at AJC of cells throughout the epithelial monolayer. MAGIs recruit ankyrin-repeat-, SH3-domain- and proline-rich-region-containing protein 2 (ASPP2) to AJC, which modulates Par-3-aPKC to antagonize ROCK-driven contractility. By coupling the adhesion machinery to the polarity proteins to regulate cellular contractility, we propose that MAGIs play essential and central roles in maintaining steady state intercellular tension throughout the epithelial cell sheet..

Kenji Matsuzawa, Takuya Himoto, Yuki Mochizuki, Junichi Ikenouchi, α-Catenin Controls the Anisotropy of Force Distribution at Cell-Cell Junctions during Collective Cell Migration., Cell reports, 10.1016/j.celrep.2018.05.070, 23, 12, 3447-3456, 2018.06, [URL], Adherens junctions (AJs) control epithelial cell behavior, such as collective movement and morphological changes, during development and in disease. However, the molecular mechanism of AJ remodeling remains incompletely understood. Here, we report that the conformational activation of α-catenin is the key event in the dynamic regulation of AJ remodeling. α-catenin activates RhoA to increase actomyosin contractility at cell-cell junctions. This leads to the stabilization of activated α-catenin, in part through the recruitment of the actin-binding proteins, vinculin and afadin. In this way, α-catenin regulates force sensing, as well as force transmission, through a Rho-mediated feedback mechanism. We further show that this is important for stable directional alignment of multiple cells during collective cell movement by both experimental observation and mathematical modeling. Taken together, our findings demonstrate that α-catenin controls the establishment of anisotropic force distribution at cell junctions to enable cooperative movement of the epithelial cell sheet..

主要学会発表等

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学会活動

所属学会名

日本分子生物学会

日本細胞生物学会

学会大会・会議・シンポジウム等における役割

2021.06.29～2021.07.02, 第73回日本細胞生物学会大会, 座長.

学術論文等の審査

年度	外国語雑誌査読論文数	合計
2022年度	3	3
2021年度	1	1
2020年度	1	1

研究資金

科学研究費補助金の採択状況(文部科学省、日本学術振興会)

2022年度～2024年度, 基盤研究(C), 代表, 運動性の細胞集団が秩序を獲得するまでの分子メカニズムの解明.

2019年度～2021年度, 基盤研究(C), 代表, 集団細胞運動における細胞接着の可塑性の制御と役割.

2018年度～2019年度, 若手研究, 代表, 張力シグナルと生化学シグナルの相互制御による集団細胞運動の協調性確立メカニズム.

九大関連コンテンツ

pure2017年10月2日から、「九州大学研究者情報」を補完するデータベースとして、Elsevier社の「Pure」による研究業績の公開を開始しました。

QIR　九州大学学術情報リポジトリシステム情報科学研究院

理学部・理学研究院


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