| Masaki Kawamata | Last modified date：2023.11.22 |
Assistant Professor /
Division of Organogenesis and Regeneration
Department of Molecular and Cellular Biology
Medical Institute of Bioregulation
Department of Molecular and Cellular Biology
Medical Institute of Bioregulation
Administration Post
Other
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Homepage
https://kyushu-u.elsevierpure.com/en/persons/masaki-kawamata
Reseacher Profiling Tool Kyushu University Pure
Phone
092-642-6449
Fax
092-642-6444
Academic Degree
Ph.D.
Country of degree conferring institution (Overseas)
No
Field of Specialization
Molecular biology
Total Priod of education and research career in the foreign country
02years09months
Outline Activities
Studying on liver regeneration and cancer, novel lineage tracing technology, and gene therapy by genome editing.
Education for students.
Education for students.
Research
Research Interests
- Development of Novel Gene Therapy by Precise Single Nucleotide Genome Editing Technology
keyword : Genome editing
2022.04～2025.03. - Development of activity regulatable CRISPR-Cas9 for gene repair therapy
keyword : CRISPR-Cas9, Genome editing、Embryonic stem cell
2017.04. - Developing a novel method to generate induced-hepatic progenitor cells
keyword : Hepatocyte, Direct reprogramming
2020.11～2021.06. - Development of a novel lineage tracing system by dual labeling method.
keyword : Lineage tracing, Genome editing
2020.04～2022.03. - Identifying cell origin of hepatocellular carcinoma
keyword : Hepatocellular carcinoma, Cancer stem cell, Lineage tracing
2016.05.
Papers
| 1. | Masaki Kawamata, Hiroshi I. Suzuki, Ryota Kimura, Atsushi Suzuki, Optimization of Cas9 activity through the addition of cytosine extensions to single-guide RNAs., Nature Biomedical Engineering, 10.1038/s41551-023-01011-7, 7, 762-691, Accepted: 17 February 2023, 2022.04, [URL], The precise regulation of the activity of Cas9 is crucial for safe and efficient editing. Here we show that the genome-editing activity of Cas9 can be constrained by the addition of cytosine stretches to the 5′-end of conventional single-guide RNAs (sgRNAs). Such a ‘safeguard sgRNA’ strategy, which is compatible with Cas12a and with systems for gene activation and interference via CRISPR (clustered regularly interspaced short palindromic repeats), leads to the length-dependent inhibition of the formation of functional Cas9 complexes. Short cytosine extensions reduced p53 activation and cytotoxicity in human pluripotent stem cells, and enhanced homology-directed repair while maintaining bi-allelic editing. Longer extensions further decreased on-target activity yet improved the specificity and precision of mono-allelic editing. By monitoring indels through a fluorescence-based allele-specific system and computational simulations, we identified optimal windows of Cas9 activity for a number of genome-editing applications, including bi-allelic and mono-allelic editing, and the generation and correction of disease-associated single-nucleotide substitutions via homology-directed repair. The safeguard-sgRNA strategy may improve the safety and applicability of genome editing.. |
| 2. | Masaki Kawamata, Takahiro Ochiya, Generation of genetically modified rats from embryonic stem cells, PNAS, 107, 32, 14223-14228, 2010.08. |
| 3. | Masaki Kawamata, Yutaka Tonomura, Tadashi Kimura, Yukihiko Sugimoto, Teruyuki Yanagisawa, Katsuhiko Nishimori, Oxytocin-induced phasic and tonic contractions are modulated by the contractile machinery rather than the quantity of oxytocin receptor, Am J Physiol Endocrinol Metab, 292, 4, 992-999, 2007.04. |
Presentations
Educational
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