Kyushu University Academic Staff Educational and Research Activities Database
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Takeshi Imai Last modified date:2019.05.29



Graduate School
Undergraduate School
Other Organization


E-Mail
Homepage
http://www.dn-en.med.kyushu-u.ac.jp/
Department of Developmental Neurophysiology .
http://researchmap.jp/takeshi.imai/?lang=english
Researchmap .
https://scholar.google.com/citations?sortby=pubdate&hl=en&user=VPwm0QUAAAAJ&view_op=list_works
Google Scholar .
https://sites.google.com/site/seedbresources/
SeeDB Resources .
Academic Degree
Ph.D.
Field of Specialization
Neuroscience
Research
Research Interests
  • Neuroscience
    keyword : Neuronal circuit formation, Olfaction
    2017.04~2017.04.
Academic Activities
Papers
1. Richi Sakaguchi, Marcus N. Leiwe, Takeshi Imai, Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags, ELIFE, 10.7554/eLife.40350, 7, 2018.11, The stochastic multicolor labeling method 'Brainbow' is a powerful strategy to label multiple neurons differentially with fluorescent proteins; however, the fluorescence levels provided by the original attempts to use this strategy were inadequate. In the present study, we developed a stochastic multicolor labeling method with enhanced expression levels that uses a tetracycline-operator system (Tetbow). We optimized Tetbow for either plasmid or virus vector-mediated multicolor labeling. When combined with tissue clearing, Tetbow was powerful enough to visualize the three-dimensional architecture of individual neurons. Using Tetbow, we were able to visualize the axonal projection patterns of individual mitral/tufted cells along several millimeters in the mouse olfactory system. We also developed a Tetbow system with chemical tags, in which genetically encoded chemical tags were labeled with synthetic fluorophores. This was useful in expanding the repertoire of the fluorescence labels and the applications of the Tetbow system. Together, these new tools facilitate light-microscopy-based neuronal tracing at both a large scale and a high resolution..