九州大学 研究者情報
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基本情報 研究活動 教育活動 社会活動
今井 猛(いまい たけし) データ更新日:2019.05.29



主な研究テーマ
神経科学
キーワード:回路形成、嗅覚
2017.04~2017.04.
研究業績
主要原著論文
1. Richi Sakaguchi, Marcus N. Leiwe, Takeshi Imai, Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags, ELIFE, 10.7554/eLife.40350, 7, 2018.11, The stochastic multicolor labeling method 'Brainbow' is a powerful strategy to label multiple neurons differentially with fluorescent proteins; however, the fluorescence levels provided by the original attempts to use this strategy were inadequate. In the present study, we developed a stochastic multicolor labeling method with enhanced expression levels that uses a tetracycline-operator system (Tetbow). We optimized Tetbow for either plasmid or virus vector-mediated multicolor labeling. When combined with tissue clearing, Tetbow was powerful enough to visualize the three-dimensional architecture of individual neurons. Using Tetbow, we were able to visualize the axonal projection patterns of individual mitral/tufted cells along several millimeters in the mouse olfactory system. We also developed a Tetbow system with chemical tags, in which genetically encoded chemical tags were labeled with synthetic fluorophores. This was useful in expanding the repertoire of the fluorescence labels and the applications of the Tetbow system. Together, these new tools facilitate light-microscopy-based neuronal tracing at both a large scale and a high resolution..
主要学会発表等
学会活動
所属学会名
Society for Neuroscience
日本神経科学学会
学協会役員等への就任
2019.05~2019.03, 日本生理学会, 評議員.
学会大会・会議・シンポジウム等における役割
2017.11~2017.11, ISMNTOP2017, organizing commettee.

九大関連コンテンツ

pure2017年10月2日から、「九州大学研究者情報」を補完するデータベースとして、Elsevier社の「Pure」による研究業績の公開を開始しました。
 
 
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