| 1. |
Le, Thi Phuc; Yu, Yang; Cho, Ik Sung; Suh, Eun Yeong; Kwon, Hyuk Chan; Shin, Sun-Ae; Park, Yong-Ho; Huh, Kang Moo, Injectable Poloxamer Hydrogel Formulations for Intratympanic Delivery of Dexamethasone, JOURNAL OF KOREAN MEDICAL SCIENCE, 10.3346/jkms.2023.38.e135, 38, 17, 2023.04. |
| 2. |
Cho, Ik Sung; Gupta, Prerak; Mostafazadeh, Nima; Wong, Sing Wan; Saichellappa, Saiumamaheswari; Lenzini, Stephen; Peng, Zhangli; Shin, Jae-Won, Deterministic Single Cell Encapsulation in Asymmetric Microenvironments to Direct Cell Polarity, ADVANCED SCIENCE, 10.1002/advs.202206014, 10, 3, 2023.01, Various signals in tissue microenvironments are often unevenly distributed around cells. Cellular responses to asymmetric cell-matrix adhesion in a 3D space remain generally unclear and are to be studied at the single-cell resolution. Here, the authors developed a droplet-based microfluidic approach to manufacture a pure population of single cells in a microscale layer of compartmentalized 3D hydrogel matrices with a tunable spatial presentation of ligands at the subcellular level. Cells elongate with an asymmetric presentation of the integrin adhesion ligand Arg-Gly-Asp (RGD), while cells expand isotropically with a symmetric presentation of RGD. Membrane tension is higher on the side of single cells interacting with RGD than on the side without RGD. Finite element analysis shows that a non-uniform isotropic cell volume expansion model is sufficient to recapitulate the experimental results. At a longer timescale, asymmetric ligand presentation commits mesenchymal stem cells to the osteogenic lineage. Cdc42 is an essential mediator of cell polarization and lineage specification in response to asymmetric cell-matrix adhesion. This study highlights the utility of precisely controlling 3D ligand presentation around single cells to direct cell polarity for regenerative engineering and medicine.. |
| 3. |
Chihiro Wakayama, Sachiko Inubushi, Tomonari Kunihisa, Sachiko Mizumoto, Motoi Baba, Hirokazu Tanino, Ik Sung Cho, Tooru Ooya, Protein corona formation on epigallocatechin gallate-Au nanoparticles suppressed tumor accumulation, JCIS Open, 2023.01. |
| 4. |
Wong, Sing Wan; Tamatam, Chandramohan R.; Cho, Ik Sung; Toth, Peter T.; Bargi, Raymond; Belvitch, Patrick; Lee, James C.; Rehman, Jalees; Reddy, Sekhar P.; Shin, Jae-Won, Inhibition of aberrant tissue remodelling by mesenchymal stromal cells singly coated with soft gels presenting defined chemomechanical cues, NATURE BIOMEDICAL ENGINEERING, 10.1038/s41551-021-00740-x, 6, 1, 54-+, 2022.01, The precise understanding and control of microenvironmental cues could be used to optimize the efficacy of cell therapeutics. Here, we show that mesenchymal stromal cells (MSCs) singly coated with a soft conformal gel presenting defined chemomechanical cues promote matrix remodelling by secreting soluble interstitial collagenases in response to the presence of tumour necrosis factor alpha (TNF-α). In mice with fibrotic lung injury, treatment with the coated MSCs maintained normal collagen levels, fibre density and microelasticity in lung tissue, and the continuous presentation of recombinant TNF-α in the gel facilitated the reversal of aberrant tissue remodelling by the cells when inflammation subsided in the host. Gel coatings with predefined chemomechanical cues could be used to tailor cells with specific mechanisms of action for desired therapeutic outcomes.. |
| 5. |
Lenzini, Stephen; Debnath, Koushik; Joshi, Jagdish C.; Wong, Sing Wan; Srivastava, Kriti; Geng, Xue; Cho, Ik Sung; Song, Angela; Bargi, Raymond; Lee, James C.; Mo, Gary C. H.; Mehta, Dolly; Shin, Jae-Won, Cell-Matrix Interactions Regulate Functional Extracellular Vesicle Secretion from Mesenchymal Stromal Cells, ACS NANO, 10.1021/acsnano.1c03231, 15, 11, 17439-17452, 2021.11. |
| 6. |
Oh, Hye Min; Kang, Eunae; Li, Zhengzheng; Cho, K. Sung; Kim, Da Eun; Mallick, Sudipta; Kang, Sun-Woong; Roh, Kyung-Ho; Huh, Kang Moo, Preparation and characterization of an in situ crosslinkable glycol chitosan thermogel for biomedical applications, JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, 10.1016/j.jiec.2019.07.002, 80, 820-828, 2019.12. |
| 7. |
I.S. Cho, T. Ooya, Cell‐Encapsulating Hydrogel Puzzle: Polyrotaxane‐Based Self‐Healing Hydrogels, Chemistry–A European Journal, 2019.11. |
| 8. |
I.S. Cho, T. Ooya, Tuned cell attachments by double-network hydrogels consisting of glycol chitosan, carboxylmethyl cellulose and agar bearing robust and self-healing properties, International Journal of Biological Macromolecules , 2019.08. |
| 9. |
I.S. Cho, H.M. Oh, M.O. Cho, B.S Jang, J.K. Cho, K.H. Park, S. W. Kang, K.M. huh, Synthesis and characterization of thiolated hexanoyl glycol chitosan as a mucoadhesive thermogelling polymer, Biomaterials research, 2018.09. |
| 10. |
I.S. Cho, T. Ooya, A Supramolecular Hydrogel Based on Polyglycerol Dendrimer‐Specific Amino Group Recognition, Chemistry–An Asian Journal, 2018.07. |
| 11. |
Li, Zhengzheng; Shim, Hyeeun; Cho, Myeong Ok; Cho, Ik Sung; Lee, Jin Hyun; Kang, Sun-Woong; Kwon, Bosun; Huh, Kang Moo, Thermo-sensitive injectable glycol chitosan-based hydrogel for treatment of degenerative disc disease, CARBOHYDRATE POLYMERS, 10.1016/j.carbpol.2018.01.006, 184, 342-353, 2018.03. |
| 12. |
I.S. Cho, T. Ooya, An injectable and self-healing hydrogel for spatiotemporal protein release via fragmentation after passing through needles, Journal of biomaterials science, polymer Edition, 2018.01. |
| 13. |
Cho, Myeong Ok; Li, Zhengzheng; Shim, Hye-Eun; Cho, Ik-Sung; Nurunnabi, Md; Park, Honghyun; Lee, Kuen Yong; Moon, Sung-Hwan; Kim, Ki-Suk; Kang, Sun-Woong; Huh, Kang Moo, Bioinspired tuning of glycol chitosan for 3D cell culture, NPG ASIA MATERIALS, 10.1038/am.2016.130, 8, 2016.09. |
| 14. |
Cho, Ik Sung; Park, Chun Gwon; Huh, Beom Kang; Cho, Myeong Ok; Khatun, Zehedina; Li, Zhengzheng; Kang, Sun-Woong; Bin Choy, Young; Huh, Kang Moo, Thermosensitive hexanoyl glycol chitosan-based ocular delivery system for glaucoma therapy, ACTA BIOMATERIALIA, 10.1016/j.actbio.2016.05.011, 39, 124-132, 2016.07. |
| 15. |
Cho, Ik Sung; Cho, Myeong Ok; Li, Zhengzheng; Nurunnabi, Md; Park, Sung Young; Kang, Sun-Woong; Huh, Kang Moo, Synthesis and characterization of a new photo-crosslinkable glycol chitosan thermogel for biomedical applications, CARBOHYDRATE POLYMERS, 10.1016/j.carbpol.2016.02.029, 144, 59-67, 2016.06. |
| 16. |
You, Ho Seon; Cho, Myeong Ok; Cho, Ik Sung; Li, Zhengzheng; Kim, Nam-Hong; Jang, Mi-Kyeong; Kang, Sun-Woong; Huh, Kang Moo, Synthesis of N-Acetylated Glycol Chitosans and Their Thermo-sensitive Sol-Gel Transition Properties, POLYMER-KOREA, 10.7317/pk.2016.40.2.328, 40, 2, 328-335, 2016.03. |
