Updated on 2025/06/13

写真a

 
KAWAI TAKAYUKI
 
Organization
Faculty of Science Department of Chemistry Associate Professor
School of Sciences Department of Chemistry(Concurrent)
Graduate School of Sciences Department of Chemistry(Concurrent)
Title
Associate Professor
Profile
I have studied in wide research fields such as molecular biology, analytical chemistry, and microfluidic engineering. When I was a Ph.D. student or a postdoctoral researcher (2007-2013), I studied basic analytical chemistry and developed new methodologies to improve analytical performance like sensitivity for capillary electrophoresis, microfluidic devices, and mass spectrometry. Next, I studied abroad in University of Illinois under the supervision of Prof. Jonathan V. Sweedler (2013-2014), where I learned how to apply basic analytical methodologies to actual biological research. After I returned to Japan as a research scientist in RIKEN (2014-2020), I integrated these basic analytical technologies and re-designed the total analysis system with engineering-driven optimization. Finally, I achieved “trace omics analysis system” that provided comprehensive molecular profiles of metabolites and glycans from even a single cell. In 2021, I was promoted to an Associate Professor in Kyushu University and started bioanalytical chemistry research with Prof. Nobuaki Matsumori. My aim is to approach micro/nano-scale life mechanisms by analyzing molecular transitions associated with important life phenomena. My interest also includes application of basic technologies to medical/pharmaceutical research. For example, pathogenesis such as cancers and dementia are induced by the disruption of life functions. By comprehensively analyzing molecular disorders, we will support elucidation of pathogenic molecular mechanism, which will lead to the development of new diagnostic method and/or new effective drugs. I hope to collaborate with you for developing novel bioanalytical methods that do not only elucidate complicated life system but also contribute to the people’s longevity and healthy life.

Research Areas

  • Nanotechnology/Materials / Nano/micro-systems

  • Nanotechnology/Materials / Nanobioscience

  • Nanotechnology/Materials / Analytical chemistry

  • Life Science / Pharmaceutical analytical chemistry and physicochemistry

Degree

  • Ph.D. in Engineering (Kyoto University)

Research History

  • 京都大学 (JSPS特別研究員 DC1) 産業技術総合研究所 (特別研究員) 名古屋大学 (JSPS特別研究員 PD) 理化学研究所 (研究員・基礎科学特別研究員) 科学技術振興機構 (さきがけ研究者)   

Education

  • Kyoto University   大学院工学研究科  

    2007.4 - 2012.3

  • The University of Tokyo   農学部  

    2002.4 - 2006.3

Research Interests・Research Keywords

  • Research theme: Development and Application of Trace Bioanalysis System

    Keyword: capillary electrophoresis, mass spectrometry, microfluidics, omics analysis, single cell, single molecule

    Research period: 2021.1

Awards

  • バイオインダストリー奨励賞

    2024.10   (一財) バイオインダストリー協会   次世代創薬を加速する超高感度キャピラリー電気泳動分析技術の開発

    川井 隆之

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    2017 年に (一財) バイオインダストリー協会が 30 周年を迎えたのを機に、次の 30 年を見据えて "最先端の研究が世界を創る-バイオテクノロジーの新時代-" をスローガンとして、バイオインダストリー大賞と共に設立されたものです。「奨励賞」は、バイオサイエンス、バイオテクノロジーに関連する応用を指向した研究に携わる有望な若手研究者に与えられます。

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  • 文部科学大臣表彰 若手科学者賞

    2022.4   文部科学省   The Young Scientists’ Award, The Commendation for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology

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    質量分析技術の発展により網羅的なバイオ分析が一般化されつつあるが、一細胞レベルの網羅分析に必要な感度を持つ汎用的な手法は存在せず、複雑な細胞ネットワークの中で生体分子や薬剤がどのように機能しているかは未解明であった。
    氏は、分子を精密に制御可能な革新的な電気泳動技術を新開発し、試料を濃縮することで超高感度なバイオ分析を実現することを着想した。従来の電気泳動法は濃縮効率が悪く、また制限も多くバイオ分析には不向きだったが、独自の二重濃縮技術を新開発することでzmol (10-21 mol) レベルの超高感度を実現した。
    本研究成果は、生体分子や薬剤の動態を一細胞レベルの微小スケールで評価することを可能にし、医療・創薬を含む幅広い生命科学領域の発展に貢献できると期待される。

  • 奨励賞 (服部賞)

    2021.7   日本電気泳動学会   Young Investigator Award

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    「革新的オンライン試料濃縮技術に基づく超高感度キャピラリー電気泳動法の開発と応用」という業績名において第22回電気泳動学会服部賞(奨励賞)を受賞した。

  • 奨励賞

    2017.9   日本分析化学会   Award for Young Researchers

  • 奨励賞

    2016.11   クロマトグラフィー科学会   Encouragement Award

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Papers

  • Online desalting of glycans labeled with 8-aminopyrene-1,3,6-trisulfonic acid via capillary electrophoresis Reviewed

    Chenchen Liu, Takayuki Kawai

    Talanta   296   128429   2025.6

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.talanta.2025.128429

  • Recent advances in capillary electrophoresis-mass spectrometry analysis of ‍trace biomolecules

    Kawai Takayuki

    Folia Pharmacologica Japonica   159 ( 5 )   321 - 326   2024.9   ISSN:00155691 eISSN:13478397

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    Language:Japanese   Publisher:The Japanese Pharmacological Society  

    <p>In recent years, various trace bioanalysis methods have been developed, including single-cell transcriptome analysis methods. As the sample volume and amount of biomolecules contained therein are extremely limited, development of new single-cell analysis methods require extremely high-level techniques. It is necessary to design an appropriate analysis system that integrates a highly sensitive detection system and a pretreatment protocol for minimizing sample loss, where separation method is especially important for analyzing diverse mixtures of biomolecules. Among them, capillary electrophoresis (CE) can separate biomolecules in nanoliter-scale solutions with high resolution, making it highly compatible with trace samples such as single cells. By combining with highly sensitive nano-electrospray ionization-mass spectrometry (MS), it is possible to detect nanomolar to sub-nanomolar biomolecules, which can be further improved by using online sample preconcentration methods. These highly sensitive analytical techniques have made it possible to analyze trace amounts of metabolites, proteins, lipids, etc. This review paper summarizes the research on CE-MS trace bioanalysis that has been reported to date, with a focus on single-cell analysis.</p>

    DOI: 10.1254/fpj.24036

    Scopus

    PubMed

    CiNii Research

  • Highly Sensitive Two-dimensional Profiling of N-linked Glycans by Hydrophilic Interaction Liquid Chromatography and Dual Stacking Capillary Gel Electrophoresis Reviewed

    Takaya Miki, Sachio Yamamoto, Chenchen Liu, Kohei Torikai, Mitsuhiro Kinoshita, Nobuaki Matsumori, Takayuki Kawai

    Analytica Chimica Acta   1320   342990 - 342990   2024.9   ISSN:0003-2670 eISSN:1873-4324

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    Background: N-Glycosylation is one of the most important post-translational modifications in proteins. As the N-glycan profiles in biological samples are diverse and change according to the pathological condition, various profiling methods have been developed, such as liquid chromatography (LC), capillary electrophoresis (CE), and mass spectrometry. However, conventional analytical methods have limitations in sensitivity and/or resolution, hindering the discovery of minor but specific N-glycans that are important both in the basic glycobiology research and in the medical application as biomarkers. Therefore, a highly sensitive and high-resolution N-glycan profiling method is required. Results: In this study, we developed a novel two-dimensional (2D) separation system, which couples hydrophilic interaction liquid chromatography (HILIC) with capillary gel electrophoresis (CGE) via large-volume dual preconcentration by isotachophoresis and stacking (LDIS). Owing to the efficient preconcentration efficiency of LDIS, limit of detection reached 12 pM (60 amol, S/N = 3) with good calibration curve linearity (R2 > 0.999) in the 2D analysis of maltoheptaose. Finally, 2D profiling of N-glycans obtained from standard glycoproteins and cell lysates were demonstrated. High-resolution 2D profiles were successfully obtained by data alignment using triple internal standards. N-glycans were well distributed on the HILIC/CGE 2D plane based on the glycan size, number of sialic acids, linkage type, and so on. As a result, specific minor glycans were successfully identified in HepG2 and HeLa cell lysates. Significance and novelty: In conclusion, the HILIC/CGE 2D analysis method showed sufficient sensitivity and resolution for identifying minor but specific N-glycans from complicated cellular samples, indicating the potential as a next-generation N-glycomics tool. Our novel approach for coupling LC and CE can also dramatically improve the sensitivity in other separation modes, which can be a new standard of 2D bioanalysis applicable not only to glycans, but also to other diverse biomolecules such as metabolites, proteins, and nucleic acids.

    DOI: 10.1016/j.aca.2024.342990

    Web of Science

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  • Application of on-line sample preconcentration by large-volume dual preconcentration by isotachophoresis and stacking (LDIS) on straight-channel microchips. International journal

    Fumihiko Kitagawa, Kazuki Takahashi, Reina Osanai, Ryota Sasaki, Takayuki Kawai

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   40 ( 9 )   1611 - 1617   2024.9   ISSN:0910-6340 eISSN:1348-2246

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Analytical Sciences  

    In this study, large-volume dual preconcentration by isotachophoresis and stacking (LDIS) which is an on-line sample preconcentration technique coupling large-volume sample stacking with an electroosmotic flow pump (LVSEP) with transient isotachophoresis (tITP) was applied to microchip electrophoresis (MCE) for improving both detection sensitivities and peak shapes. To realize LDIS in MCE, we investigated experimental procedures for injecting a short plug of a leading electrolyte (LE) solution into a straight microchannel without any sophisticated injector apparatus. We found that a short LE plug could be injected into a sample-filled straight-channel only by making the liquid level of the LE solution in an outlet reservoir higher than that in an inlet one. By applying a reversed-polarity voltage to the microchip, anionic analytes injected throughout the microchannel were first enriched by LVSEP, followed by tITP. Through the second preconcentration effect by tITP in LDIS, sensitivity enhancement factor (SEF) and asymmetry factor for a standard dye were improved from 878 and 0.62 to 1330 and 1.14, respectively, relative to those in conventional LVSEP. It should be noted that more viscous running buffer containing sieving polymers could be employed to the LDIS analysis, which was effective for improving the SEF and the separation efficiencies, especially for bio-polymeric compounds. Finally, LDIS was applied to the oligosaccharide and protein analyses in MCE, resulting in the SEFs of 1410 and ca. 50 for maltotriose and bovine milk casein, respectively.

    DOI: 10.1007/s44211-024-00597-5

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  • Effect of Methanol and Polymer Additives on Large-Volume Dual Preconcentration by Isotachophoresis and Stacking (LDIS) in Straight-Microchannels

    KITAGAWA Fumihiko, KUDO Ayaka, KAWAI Takayuki

    CHROMATOGRAPHY   advpub ( 0 )   2024.8   ISSN:13428284 eISSN:13483315

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    Language:English   Publisher:The Society for Chromatographic Sciences  

    <p>In our previous study, we found that the combination of large-volume sample stacking with an electroosmotic flow pump (LVSEP) and transient isotachophoresis (tITP), which named large volume dual preconcentration by isotachophoresis and stacking (LDIS), was successfully applied to microchip electrophoresis (MCE). In MCE-LDIS, highly viscous background electrolytes (BGEs) containing sieving polymers such as hydroxypropyl(methyl cellulose) (HPMC) could be employed, which should be effective for concentrating and separating especially for bio-polymeric compounds such as proteins and glycans. To adjust both enrichment efficiencies and resolutions in MCE-LDIS, in this study, the effects of methanol and HPMC additives in the BGE were investigated. In the BGE containing no HPMC, the peak height of a standard dye was increased upon increasing the methanol contents from 0 to 20%, while from 20 to 60% that gradually became smaller. Since the viscosity of water was increased by the addition of 20% methanol from 1.0 to 1.6 cP, the band broadening of the focused analytes would be suppressed, improving the sensitivity enhancement factor (SEF) from 1550 to 8250. Furthermore, the use of the BGE containing both 10% methanol and 1.5% HPMC in LDIS gave the best SEF of 18300 for the standard dye. Addition of methanol and HPMC was also effective for the separation of oligosaccharides, improving the resolutions from 0.85~1.25 to 1.56~1.73. It was revealed that, therefore, methanol and polymer additives were quite important factor for adjusting both SEFs and resolutions in MCE-LDIS.</p>

    DOI: 10.15583/jpchrom.2024.013

    CiNii Research

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Books

  • CE-MS Approaches for Single Cell Metabolomics in a Book "Capillary Electrophoresis - Mass Spectrometry for Proteomics and Metabolomics: Principles and Applications"

    Kawai Takayuki

    Wiley  2022    ISBN:9783527349210

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    Total pages:400  

    CiNii Research

  • Free d-Aspartate in Nonmammalian Animals: Detection, Localization, Metabolism, and Function

    Amit V. Patel, Takayuki Kawai, Stanislav S. Rubakhin, Jonathan V. Sweedler

    Springer  2016.8 

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    Language:Others  

    Free d-Aspartate in Nonmammalian Animals: Detection, Localization, Metabolism, and Function

  • 食のバイオ計測の最前線―機能解析と安全・安心の計測を目指して―

    北川 文彦, 川井 隆之, 大塚 浩二(Role:Joint author)

    CMC出版  2011.5 

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    Responsible for pages:総ページ数:277   Language:Japanese  

    Frontier of Biomeasurement in Food Sciences ―Analysis of Function and Measurement with Accountability and Reliability

Presentations

  • 超高感度・極微量分析技術が切り拓く次世代創薬研究 Invited

    川井 隆之

    徳島大学大学院医歯薬学研究部 DDS研究センター 2024年度 第2回シンポジウム  2025.1 

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    Event date: 2025.1

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

  • 超高感度糖鎖分析法の開発(仮題) Invited

    川井 隆之

    第47回 日本分子生物学会年会@福岡国際会議場  2024.11 

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    Event date: 2024.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡国際会議場   Country:Japan  

  • サブセルラー質量分析技術が切り拓く生体膜研究 Invited

    川井 隆之

    日本膜学会第45年会・膜シンポジウム2023合同大会  2023.11 

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    Event date: 2023.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:早稲田大学リサーチイノベーションセンター   Country:Japan  

  • 超高感度CE-MS分析法による脳分子探査 Invited

    川井 隆之

    第96回日本生化学会大会  2023.11 

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    Event date: 2023.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡国際会議場   Country:Japan  

  • Ultra-sensitive Bioanalysis by Dual-Stacking Capillary Electrophoresis Invited International conference

    Takayuki Kawai

    19th Asia-Pacific International Symposium on Microscale Separations and Analysis 2023 (APCE 2023)  2023.10 

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    Event date: 2023.10

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Kuala Lumpur, Malaysia   Country:Japan  

    Capillary electrophoresis (CE) is an efficient separation technique for biomolecules, however, concentration sensitivity is usually very low because the sample injection volume is quite limited (~nL). To address this issue, online sample preconcentration (OSP) methods have been developed. However, OSP methods often provide only 100-fold or poorer sensitivity improvement in exchange for the loss of resolution, requiring cumbersome optimization for each type of sample. Thus, it is rare to use OSP methods for routine CE bioanalysis. Here, we developed two types of novel OPS methods, large-volume dual preconcentration by isotachophoresis and stacking (LDIS) and large-volume dual preconcentration by micelle collapse and sweeping (LDMS).
    LDIS was designed for the analysis of charged molecules, which was firstly applied to N-linked glycans. Owing to the dual stacking mechanism, 2,000-fold sensitivity improvement was achieved without losing the resolution at all. Finally, the around 40 glycans were successfully profiled from quite limited number of cells (~100). LDIS was also applied to metabolome analysis. Single HeLa cells were collected by micro-manipulation and analysed by LDIS-CE-mass spectrometry (MS). Forty metabolites were profiled from a single HeLa cell and limit of detection of 450 fM (10–15 M, S/N = 3) was achieved.
    LDMS was designed for uncharged hydrophobic compounds like drugs. DXd, an efficient anti-cancer payload, was extracted from a tissue slice and analysed by LDMS-CE-MS using sodium dodecyl sulfate micelle as pseudo-stationary phase. DXd was trapped by the micelle and eluted by acetonitrile-induced micelle collapse, resulting in 1,000-fold preconcentration and limit of quantification of 420 fM (S/N = 10). Since the hydrophilic metabolites were not trapped by the micelle, DXd was separated from the cellular contaminants, and attomole-level of DXd was successfully quantified from tissue microsections,

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MISC

  • 超高感度質量分析技術が切り拓くミクロスケール薬物動態研究 Reviewed

    川井 隆之

    膜   2024.3

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • 超高感度キャピラリー電気泳動による微量バイオ分析法の開発

    川井 隆之

    分析化学   72 ( 9 )   349 - 355   2023.9   ISSN:0525-1931

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    Language:Japanese   Publisher:(公社)日本分析化学会  

    キャピラリー電気泳動(CE)は高効率な分離法であり,レーザー励起蛍光や質量分析などの高感度検出法と組み合わせることで,nLオーダーの試料溶液に含まれるzmolレベルの生体分子を検出できる.しかし一般的な生体試料の分析では,前処理においてμLオーダーの溶液を調製する必要があり,nLオーダーの試料しか注入できないCEではほとんどの溶液を無駄にしてしまい,CEの分析性能を活かすことができない.そこで著者は,CEにおいてμLオーダーの大量の試料を導入できる新規二重オンライン試料濃縮法を開発し,生体分子や薬剤などを対象に1000倍前後の濃縮を実現してきた.これらの濃縮法を用いることで,1~100細胞程度の培養細胞や組織切片に含まれる糖鎖・代謝物・薬剤などを分析することに成功した.本論文ではこれらの研究成果について紹介する.(著者抄録)

  • マイクロ流路残留圧力を利用したゲルキャスティング

    田中信行, 森口裕之, 佐藤麻子, 川井隆之, 田中陽

    化学とマイクロ・ナノシステム学会研究会講演要旨集   2015.11

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    Language:Japanese  

    マイクロ流路残留圧力を利用したゲルキャスティング

  • 酸素プラズマパターニングを利用したシンプルなPDMSバルブの開発

    森口裕之, 川井隆之, 神田元紀, 山田陸裕, 上田泰己, 田中陽

    化学とマイクロ・ナノシステム学会研究会講演要旨集   2014.10

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    Language:Japanese  

    酸素プラズマパターニングを利用したシンプルなPDMSバルブの開発

Industrial property rights

Patent   Number of applications: 3   Number of registrations: 1
Utility model   Number of applications: 0   Number of registrations: 0
Design   Number of applications: 0   Number of registrations: 0
Trademark   Number of applications: 0   Number of registrations: 0

Professional Memberships

  • SOCIETY FOR CHROMATOGRAPHIC SCIENCES

  • THE SOCIETY FOR CHEMISTRY AND MICRO-NANO SYSTEMS

  • THE JAPAN SOCIETY FOR ANALYTICAL CHEMISTRY

  • THE CHEMICAL SOCIETY OF JAPAN

  • JAPANESE ELECTROPHORESIS SOCIETY

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Committee Memberships

  • 日本分析化学会 近畿支部   常任幹事   Domestic

    2020.4 - 2020.12   

  • 日本電気泳動学会   Councilor   Domestic

    2019.7 - Present   

  • 日本分析化学会 近畿支部   Organizer   Domestic

    2015.4 - 2020.3   

Academic Activities

  • 第62回 化学関連支部合同九州大会 実行委員

    Role(s): Planning, management, etc.

    椿 俊太郎  2025.7

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    Type:Competition, symposium, etc. 

  • 第74回日本電気泳動学会シンポジウム 世話人 (実行委員長)

    川井 隆之  ( Web Japan ) 2024.9

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    Type:Competition, symposium, etc. 

  • Analytical Sciences Associate Editor International contribution

    2022.3 - 2026.3

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    Type:Academic society, research group, etc. 

  • 第42回キャピラリー電気泳動シンポジウム 実行委員

    加地 範匡  ( Japan ) 2021.12

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    Type:Competition, symposium, etc. 

Research Projects

  • 臨床検査応用に向けた超高感度CE-MSメタボローム分析法の開発

    2024

    日本医療研究開発機構 (AMED) 橋渡し研究プログラム シーズH

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    Authorship:Principal investigator  Grant type:Contract research

  • 超高感度キャピラリー電気泳動技術を基盤とした次世代グライコシーケンサーの開発

    Grant number:23H02623  2023 - 2026

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 超高感度 CE-MS 薬物動態解析に基づくドラッグデリバリー創薬支援事業

    2023

    九州大学 ギャップファンド

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    Authorship:Principal investigator  Grant type:On-campus funds, funds, etc.

  • 生体膜ナノ構造の可視化と脂質網羅分析のための統合ナノ分析システム

    Grant number:22K18950  2022 - 2023

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Challenging Research(Exploratory)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 超高感度キャピラリー電気泳動法を用いた受託分析サービスとその多角展開

    2022

    日本科学技術振興機構 (JST) 研究成果展開事業 大学発新産業創出プログラム (START) 大学・エコシステム推進型 スタートアップ・エコシステム形成支援 (PARKS)

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    Authorship:Principal investigator  Grant type:Contract research

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Educational Activities

  • Lecture and practice in both graduate undergraduate schools. Special research education in the laboratory of bioanalytical chemistry. Join some committees in the university.

Class subject

  • 分析化学実験

    2025.10 - 2025.11   Second semester

  • 分析化学特論IIB

    2025.6 - 2025.8   Summer quarter

  • 国際化学特論III

    2025.6 - 2025.8   Summer quarter

  • 分析化学I

    2025.4 - 2025.8   First semester

  • 自然科学総合実験

    2024.10 - 2025.3   Second semester

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Teaching Student Awards

  • ポスター賞

    Year and month of award:2024.12

    Classification of award-winning students:Postgraduate student   Name of award-winning student:山下 愛斗

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  • ポスター賞

    Year and month of award:2024.12

    Classification of award-winning students:Postgraduate student   Name of award-winning student:何 甦恩

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  • 優秀講演賞

    Year and month of award:2024.10

    Classification of award-winning students:Postgraduate student   Name of award-winning student:猪狩 世玲菜

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  • ポスター賞

    Year and month of award:2024.10

    Classification of award-winning students:Postgraduate student   Name of award-winning student:Haotian Rong

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  • ポスター賞

    Year and month of award:2024.9

    Classification of award-winning students:Postgraduate student   Name of award-winning student:猪狩 世玲菜

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