Updated on 2024/10/04

Information

 

写真a

 
KAWAI TAKAYUKI
 
Organization
Faculty of Science Department of Chemistry Associate Professor
School of Sciences Department of Chemistry（Joint Appointment）
Graduate School of Sciences Department of Chemistry（Joint Appointment）
Title
Associate Professor
Profile
I have studied in wide research fields such as molecular biology, analytical chemistry, and microfluidic engineering. When I was a Ph.D. student or a postdoctoral researcher (2007-2013), I studied basic analytical chemistry and developed new methodologies to improve analytical performance like sensitivity for capillary electrophoresis, microfluidic devices, and mass spectrometry. Next, I studied abroad in University of Illinois under the supervision of Prof. Jonathan V. Sweedler (2013-2014), where I learned how to apply basic analytical methodologies to actual biological research. After I returned to Japan as a research scientist in RIKEN (2014-2020), I integrated these basic analytical technologies and re-designed the total analysis system with engineering-driven optimization. Finally, I achieved “trace omics analysis system” that provided comprehensive molecular profiles of metabolites and glycans from even a single cell. In 2021, I was promoted to an Associate Professor in Kyushu University and started bioanalytical chemistry research with Prof. Nobuaki Matsumori. My aim is to approach micro/nano-scale life mechanisms by analyzing molecular transitions associated with important life phenomena. My interest also includes application of basic technologies to medical/pharmaceutical research. For example, pathogenesis such as cancers and dementia are induced by the disruption of life functions. By comprehensively analyzing molecular disorders, we will support elucidation of pathogenic molecular mechanism, which will lead to the development of new diagnostic method and/or new effective drugs. I hope to collaborate with you for developing novel bioanalytical methods that do not only elucidate complicated life system but also contribute to the people’s longevity and healthy life.
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Degree

  • Ph.D. in Engineering (Kyoto University)

Research History

  • 京都大学 (JSPS特別研究員 DC1) 産業技術総合研究所 (特別研究員) 名古屋大学 (JSPS特別研究員 PD) 理化学研究所 (研究員・基礎科学特別研究員) 科学技術振興機構 (さきがけ研究者)

Research Interests・Research Keywords

  • Research theme：Development and Application of Trace Bioanalysis System

    Keyword：capillary electrophoresis, mass spectrometry, microfluidics, omics analysis, single cell, single molecule

    Research period： 2021.1

Awards

  • バイオインダストリー奨励賞

    2024.10   (一財) バイオインダストリー協会   次世代創薬を加速する超高感度キャピラリー電気泳動分析技術の開発

    川井 隆之

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  • 文部科学大臣表彰 若手科学者賞

    2022.4   文部科学省   革新的電気泳動技術に基づく超高感度バイオ分析研究

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    質量分析技術の発展により網羅的なバイオ分析が一般化されつつあるが、一細胞レベルの網羅分析に必要な感度を持つ汎用的な手法は存在せず、複雑な細胞ネットワークの中で生体分子や薬剤がどのように機能しているかは未解明であった。
    氏は、分子を精密に制御可能な革新的な電気泳動技術を新開発し、試料を濃縮することで超高感度なバイオ分析を実現することを着想した。従来の電気泳動法は濃縮効率が悪く、また制限も多くバイオ分析には不向きだったが、独自の二重濃縮技術を新開発することでzmol (10-21 mol) レベルの超高感度を実現した。
    本研究成果は、生体分子や薬剤の動態を一細胞レベルの微小スケールで評価することを可能にし、医療・創薬を含む幅広い生命科学領域の発展に貢献できると期待される。

  • 奨励賞 (服部賞)

    2021.7   日本電気泳動学会   革新的オンライン試料濃縮技術に基づく超高感度キャピラリー電気泳動法の開発と応用

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    「革新的オンライン試料濃縮技術に基づく超高感度キャピラリー電気泳動法の開発と応用」という業績名において第22回電気泳動学会服部賞（奨励賞）を受賞した。

  • 奨励賞

    2017.9   日本分析化学会   オンライン試料濃縮法を駆使した簡便かつ高感度なミクロスケール電気泳動システムの創出

  • 奨励賞

    2016.11   クロマトグラフィー科学会   簡便かつ高性能なミクロスケール分析システムの開発

  • 若手優秀賞

    2016.4   化学とマイクロ・ナノシステム学会   簡便かつ高性能なミクロスケール分析技術の創成

  • Prize for the Best Oral Presentation

    2014.12   Sample Treatment 2014   Prize for the Best Oral Presentation

  • PSC Young Scientist Lecture Award (Level 1)

    2011.11   HPLC 2011 Dalian   PSC Young Scientist Lecture Award (Level 1)

  • Poster Presentation Award

    2011.9   JAIMA Conference 2011  

  • 若手講演賞

    2011.9   日本分析化学会  

  • Student Poster Award

    2010.12   PACIFICHEM 2010  

  • 若手優秀賞

    2010.8   分析化学会近畿支部  

  • 最優秀奨励テーマ賞

    2010.3   京都高度技術研究所  

  • Halász Award

    2009.6  

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Papers

  • Recent advances in capillary electrophoresis-mass spectrometry analysis of ‍trace biomolecules

    Kawai Takayuki

    Folia Pharmacologica Japonica   159 ( 5 )   321 - 326   2024.9   （ ISSN:00155691 eISSN:13478397 ）

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    Language：Japanese   Publisher：The Japanese Pharmacological Society  

    <p>In recent years, various trace bioanalysis methods have been developed, including single-cell transcriptome analysis methods. As the sample volume and amount of biomolecules contained therein are extremely limited, development of new single-cell analysis methods require extremely high-level techniques. It is necessary to design an appropriate analysis system that integrates a highly sensitive detection system and a pretreatment protocol for minimizing sample loss, where separation method is especially important for analyzing diverse mixtures of biomolecules. Among them, capillary electrophoresis (CE) can separate biomolecules in nanoliter-scale solutions with high resolution, making it highly compatible with trace samples such as single cells. By combining with highly sensitive nano-electrospray ionization-mass spectrometry (MS), it is possible to detect nanomolar to sub-nanomolar biomolecules, which can be further improved by using online sample preconcentration methods. These highly sensitive analytical techniques have made it possible to analyze trace amounts of metabolites, proteins, lipids, etc. This review paper summarizes the research on CE-MS trace bioanalysis that has been reported to date, with a focus on single-cell analysis.</p>

    DOI： 10.1254/fpj.24036

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  • Highly Sensitive Two-dimensional Profiling of N-linked Glycans by Hydrophilic Interaction Liquid Chromatography and Dual Stacking Capillary Gel Electrophoresis Reviewed

    Takaya Miki, Sachio Yamamoto, Chenchen Liu, Kohei Torikai, Mitsuhiro Kinoshita, Nobuaki Matsumori, Takayuki Kawai

    Analytica Chimica Acta   1320   342990 - 342990   2024.9   （ ISSN:0003-2670 eISSN:1873-4324 ）

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.aca.2024.342990

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  • Application of on-line sample preconcentration by large-volume dual preconcentration by isotachophoresis and stacking (LDIS) on straight-channel microchips. International journal

    Fumihiko Kitagawa, Kazuki Takahashi, Reina Osanai, Ryota Sasaki, Takayuki Kawai

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   40 ( 9 )   1611 - 1617   2024.9   （ ISSN:0910-6340 eISSN:1348-2246 ）

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    Language：English   Publishing type：Research paper (scientific journal)  

    In this study, large-volume dual preconcentration by isotachophoresis and stacking (LDIS) which is an on-line sample preconcentration technique coupling large-volume sample stacking with an electroosmotic flow pump (LVSEP) with transient isotachophoresis (tITP) was applied to microchip electrophoresis (MCE) for improving both detection sensitivities and peak shapes. To realize LDIS in MCE, we investigated experimental procedures for injecting a short plug of a leading electrolyte (LE) solution into a straight microchannel without any sophisticated injector apparatus. We found that a short LE plug could be injected into a sample-filled straight-channel only by making the liquid level of the LE solution in an outlet reservoir higher than that in an inlet one. By applying a reversed-polarity voltage to the microchip, anionic analytes injected throughout the microchannel were first enriched by LVSEP, followed by tITP. Through the second preconcentration effect by tITP in LDIS, sensitivity enhancement factor (SEF) and asymmetry factor for a standard dye were improved from 878 and 0.62 to 1330 and 1.14, respectively, relative to those in conventional LVSEP. It should be noted that more viscous running buffer containing sieving polymers could be employed to the LDIS analysis, which was effective for improving the SEF and the separation efficiencies, especially for bio-polymeric compounds. Finally, LDIS was applied to the oligosaccharide and protein analyses in MCE, resulting in the SEFs of 1410 and ca. 50 for maltotriose and bovine milk casein, respectively.

    DOI： 10.1007/s44211-024-00597-5

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  • Effect of Methanol and Polymer Additives on Large-Volume Dual Preconcentration by Isotachophoresis and Stacking (LDIS) in Straight-Microchannels

    KITAGAWA Fumihiko, KUDO Ayaka, KAWAI Takayuki

    CHROMATOGRAPHY   advpub ( 0 )   2024.8   （ ISSN:13428284 eISSN:13483315 ）

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    Language：English   Publisher：The Society for Chromatographic Sciences  

    <p>In our previous study, we found that the combination of large-volume sample stacking with an electroosmotic flow pump (LVSEP) and transient isotachophoresis (tITP), which named large volume dual preconcentration by isotachophoresis and stacking (LDIS), was successfully applied to microchip electrophoresis (MCE). In MCE-LDIS, highly viscous background electrolytes (BGEs) containing sieving polymers such as hydroxypropyl(methyl cellulose) (HPMC) could be employed, which should be effective for concentrating and separating especially for bio-polymeric compounds such as proteins and glycans. To adjust both enrichment efficiencies and resolutions in MCE-LDIS, in this study, the effects of methanol and HPMC additives in the BGE were investigated. In the BGE containing no HPMC, the peak height of a standard dye was increased upon increasing the methanol contents from 0 to 20%, while from 20 to 60% that gradually became smaller. Since the viscosity of water was increased by the addition of 20% methanol from 1.0 to 1.6 cP, the band broadening of the focused analytes would be suppressed, improving the sensitivity enhancement factor (SEF) from 1550 to 8250. Furthermore, the use of the BGE containing both 10% methanol and 1.5% HPMC in LDIS gave the best SEF of 18300 for the standard dye. Addition of methanol and HPMC was also effective for the separation of oligosaccharides, improving the resolutions from 0.85~1.25 to 1.56~1.73. It was revealed that, therefore, methanol and polymer additives were quite important factor for adjusting both SEFs and resolutions in MCE-LDIS.</p>

    DOI： 10.15583/jpchrom.2024.013

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  • Combination of on-line sample preconcentration by large-volume dual preconcentration by isotachophoresis and stacking (LDIS) with field-amplified sample injection (FASI) on Y-channel microchips

    Kitagawa, F; Sato, S; Suzuki, T; Kawai, T

    ANALYTICAL SCIENCES   2024.8   （ ISSN:0910-6340 eISSN:1348-2246 ）

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    Language：English   Publisher：Analytical Sciences  

    In our previous study, the combination of two on-line sample preconcentration techniques, large-volume sample stacking with an electroosmotic flow (EOF) pump (LVSEP) and transient isotachophoresis (tITP), in microchip electrophoresis (MCE) was developed, which was named large-volume dual preconcentration by isotachophoresis and stacking (LDIS). LDIS was apparently effective for improving the sensitivity and the peak shape. In LDIS, however, there was a limit to the improvement of the sensitivity enhancement factor (SEF) since the amount of analytes to be concentrated was limited to the channel volume. To overcome this issue, in the present article, LDIS was coupled with field-amplified sample injection (FASI) technique on Y-shaped channel microchips. The use of a Y-channel in LDIS-FASI allowed consecutive LVSEP, FASI and tITP enrichments with a simple voltage control. In conventional LVSEP and LDIS analyses of a standard analyte, the SEFs were evaluated to be 2630 and 13,100, respectively, whereas in LDIS-FASI that was increased to 27,900 even at the FASI injection time of 0 s. To achieve higher SEFs, furthermore, the FASI injection time was increased to 150 s, resulting in the best SEF of 58,500. It should be emphasized that the peak width in LDIS-FASI was quite narrow, only 0.3–3.1 s, while in normal LVSEP that was 13 s. Furthermore, the LDIS-FASI technique was applied to the analysis of oligosaccharide mixture. Due to the focusing effect by LDIS-FASI, the resolutions were improved from 0.97–1.57 to 2.08–2.73. Graphical Abstract: (Figure presented.).

    DOI： 10.1007/s44211-024-00647-y

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  • Recent advances in microscale separation techniques for glycome analysis

    Chenchen Liu, Koji Otsuka, Takayuki Kawai

    Journal of Separation Science   47 ( 11 )   e2400170   2024.6   （ ISSN:1615-9306 eISSN:1615-9314 ）

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    The glycomic analysis holds significant appeal due to the diverse roles that glycans and glycoconjugates play, acting as modulators and mediators in cellular interactions, cell/organism structure, drugs, energy sources, glyconanomaterials, and more. The glycomic analysis relies on liquid‐phase separation technologies for molecular purification, separation, and identification. As a miniaturized form of liquid‐phase separation technology, microscale separation technologies offer various advantages such as environmental friendliness, high resolution, sensitivity, fast speed, and integration capabilities. For glycan analysis, microscale separation technologies are continuously evolving to address the increasing challenges in their unique manners. This review discusses the fundamentals and applications of microscale separation technologies for glycomic analysis. It covers liquid‐phase separation technologies operating at scales generally less than 100 µm, including capillary electrophoresis, nanoflow liquid chromatography, and microchip electrophoresis. We will provide a brief overview of glycomic analysis and describe new strategies in microscale separation and their applications in glycan analysis from 2014 to 2023.

    DOI： 10.1002/jssc.202400170

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  • Terahertz-capillary electrophoresis (THz-CE) for direct detection of separated substances in solutions Reviewed

    Keiko Kitagishi, Takayuki Kawai, Masayoshi Tonouchi, Kazunori Serita

    Optical Materials Express   14 ( 2 )   472 - 472   2024.1   （ ISSN:2159-3930 eISSN:2159-3930 ）

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Optica Publishing Group  

    We present a novel technique for capillary electrophoresis (CE) using terahertz (THz) waves, namely “THz-CE,” which enables us to sensitively detect separated substances in a solution flowing in a hollow of capillary whose inner diameter is smaller than 100 µm. Such THz detection could be achieved by utilizing the near-field interaction between a solution filled in a capillary and a point THz source that was locally generated by optical rectification in a nonlinear optical crystal irradiated with a femtosecond pulse laser. Here, we investigated the performance of THz-CE numerically and experimentally, and succeeded in observing the electrophoretic chromatogram for the separation between acetic acid and n-propionic acid by THz-CE.

    DOI： 10.1364/ome.500594

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    Other Link： https://opg.optica.org/viewmedia.cfm?URI=ome-14-2-472&seq=0

  • Gold nanoparticle-powered screening of membrane protein-specific lipids from complex lipid mixtures. Reviewed International journal

    Supakorn Wangamnuayporn, Masanao Kinoshita, Takayuki Kawai, Nobuaki Matsumori

    Analytical Biochemistry   687   115447 - 115447   2023.12   （ ISSN:0003-2697 eISSN:1096-0309 ）

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    Membrane proteins (MPs) are affected by binding of specific lipids. We previously developed a methodology for systematically analyzing MP-lipid interactions leveraging surface plasmon resonance (SPR). In this method, the gold sensor chip surface was modified with a self-assembled monolayer (SAM), which allowed for a larger amount of MP-immobilization. However, the laborious lipid purification step remained a bottleneck. To address this issue, a new strategy has been developed utilizing gold nanoparticles (AuNPs) instead of the gold sensor chip. AuNPs were coated with SAM, on which MP was covalently anchored. The MP-immobilized AuNPs were mixed with a lipid mixture, and the recovered lipids were quantified by LC-MS. Bacteriorhodopsin (bR) was used as an MP to demonstrate this concept. We optimized immobilization conditions and confirmed the efficient immobilization of bR by dynamic light scattering and electron micrographs. Washing conditions for pulldown experiments were optimized to efficiently remove non-specific lipids. A new binding index was introduced to qualitatively reproduce the known affinity of lipids for bR. Consequently, the low-abundant and least-studied lipid S-TeGD was identified as a candidate for bR-specific lipids. This technique can skip the laborious lipid purification process, accelerating the screening of MP-specific lipids from complex lipid mixtures.

    DOI： 10.1016/j.ab.2023.115447

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  • An innovative detection technique for capillary electrophoresis: Localized terahertz emission-time domain spectroscopy. Reviewed International journal

    Keiko Kitagishi, Takayuki Kawai, Masayoshi Tonouchi, Kazunori Serita

    Journal of Chromatography A   1710   464384 - 464384   2023.11

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    DOI： 10.1016/j.chroma.2023.464384

  • Unique applications of capillary electrophoresis. International journal

    Takayuki Kawai

    Analytical Sciences   39 ( 9 )   1433 - 1434   2023.9   （ ISSN:0910-6340 eISSN:1348-2246 ）

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  • Quantitative analysis of drug distribution in heterogeneous tissues using dual‐stacking capillary electrophoresis–mass spectrometry Reviewed

    180 ( 6 )   762 - 774   2022.12

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    腫瘍における細胞の不均一性は薬剤耐性につながることが多く，創薬における大きな課題となっている。従って，組織内の薬剤分布を明らかにすることは非常に重要であるが，腫瘍の微小領域に含まれる僅かな薬剤を定量することは従来分析法では難しかった。そこで我々は，キャピラリー電気泳動-質量分析 (CE-MS) における薬剤向けの新たな高効率試料濃縮法として，large-volume dual preconcentration by micelle collapse and sweeping (LDMS) 法を開発した。本研究では，HER3標的抗体薬物複合体であるpatritumab deruxtecan (HER3-DXd) を投与したモデル動物およびヒトから採取した組織検体におけるペイロード分子 (DXd) の分布を評価した。その結果，HER3高発現領域，隣接領域，HER3低発現領域の順にDXdが濃度勾配を描いて分布していることが分かり，これまで理論に過ぎなかった抗体薬物複合体におけるBystander効果を世界で初めて実証した。

    DOI： 10.1111/bph.15988

  • Tracking metabolites at single-cell resolution reveals metabolic dynamics during plant mitosis. Reviewed International journal

    Emiko Okubo-Kurihara, Ahmed Ali, Mika Hiramoto, Yukio Kurihara, Yasmine Abouleila, Eman Muhammad Abdelazem, Takayuki Kawai, Yuko Makita, Mika Kawashima, Tsuyoshi Esaki, Hiroaki Shimada, Tetsuya Mori, Masami Yokota Hirai, Takumi Higaki, Seiichiro Hasezawa, Yoshihiro Shimizu, Tsutomu Masujima, Minami Matsui

    Plant physiology   189 ( 2 )   459 - 464   2022.3   （ ISSN:0032-0889 eISSN:1532-2548 ）

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    One-sentence summary

    Analyzing only one cell allows the changes and characteristics of intracellular metabolites during the chromosome segregation process to be precisely captured and mitotic sub-phases to be dissected at the metabolite level.

    DOI： 10.1093/plphys/kiac093

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    Other Link： https://academic.oup.com/plphys/article-pdf/189/2/459/43914053/kiac093.pdf

  • Development of ultra-sensitive mass spectrometry techniques and its application to single cell analysis

    Kawai Takayuki

    Electrophoresis Letters   66 ( 1 )   19 - 22   2022   （ ISSN:21892628 eISSN:21892636 ）

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    Language：Japanese   Publisher：Japanese Electrophoresis Society  

    <p>In order to understand the complicated life system which is composed of trillions of cells, highly sensitive and comprehensive analysis system for single cells is needed. For this purpose, I have developed a series of microscale chemical analysis techniques such as sample collection, preparation, separation, and detection. Among them, sensitivity improvement in mass spectrometry (MS) detection has been quite important for the comprehensive bioanalysis. One approach is to perform capillary electrophoresis prior to MS detection, which can reduce the ionization suppression by its excellent separation performance and also can improve the sensitivity by online sample preconcentration methods, such as large-volume dual preconcentration by isotachophoresis and stacking. Consequently, zeptomole-level sensitivity has been realized, which has been successfully applied to single cell metabolome analysis and pharmacokinetic analysis. These results showed great potential of our technologies for contributing to the progress in the biological research fields.</p>

    DOI： 10.2198/electroph.66.19

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  • Recent advances in microscale separation techniques for lipidome analysis Invited Reviewed

    Takayuki Kawai, Nobuaki Matsumori, Koji Otsuka

    Analyst   146   7398 - 7410   2021.10

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    DOI： 10.1039/d1an00967b

  • Quantitation of Cell Membrane Permeability of Cyclic Peptides by Single-Cell Cytoplasm Mass Spectrometry Reviewed

    Takayuki Kawai, Yasuhiro Mihara, Makiko Morita, Masahiko Ohkubo, Taiji Asami, Tomonobu M. Watanabe

    Analytical Chemistry   93 ( 7 )   3370 - 3377   2021.2

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    DOI： 10.1021/acs.analchem.0c03901

  • Recent Advances in Trace Bioanalysis by Capillary Electrophoresis Invited Reviewed

    Takayuki Kawai

    Analytical Sciences   37 ( 1 )   27 - 36   2021.1

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    DOI： 10.2116/analsci.20sar12

  • 超高感度CE/MSの開発と一細胞メタボローム分析への応用 Invited Reviewed

    川井隆之

    分析化学   69 ( 12 )   753 - 757   2020.12

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  • 超高感度キャピラリー電気泳動を用いた微量オミックス分析 Invited Reviewed

    川井 隆之

    電気泳動   64 ( 1 )   23 - 26   2020.7

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    DOI： 10.2198/electroph.64.23

  • Flow analysis on microcasting with degassed polydimethylsiloxane micro-channels for cell patterning with cross-linked albumin Reviewed

    Yigang Shen, Nobuyuki Tanaka, Hironori Yamazoe, Shunsuke Furutani, Hidenori Nagai, Takayuki Kawai, Yo Tanaka

    PLoS ONE   15 ( 5 )   e0232518 - e0232518   2020.5

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    DOI： 10.1371/journal.pone.0232518

  • Ultrasensitive Single Cell Metabolomics by Capillary Electrophoresis-Mass Spectrometry with a Thin-Walled Tapered Emitter and Large-Volume Dual Sample Preconcentration Reviewed

    Takayuki Kawai, Nobutoshi Ota, Kaori Okada, Akiko Imasato, Yuri Owa, Makiko Morita, Misa Tada, Yo Tanaka

    Analytical Chemistry   91 ( 16 )   10564 - 10572   2019.8

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    DOI： 10.1021/acs.analchem.9b01578

  • Live single cell mass spectrometry reveals cancer-specific metabolic profiles of circulating tumor cells Reviewed International journal

    Yasmine Abouleila, Kaoru Onidani, Ahmed Ali, Hirokazu Shoji, Takayuki Kawai, Chwee Teck Lim, Vipin Kumar, Shinobu Okaya, Ken Kato, Eiso Hiyama, Toshio Yanagida, Tsutomu Masujima, Yoshihiro Shimizu, Kazufumi Honda

    Cancer Science   110 ( 2 )   697 - 706   2019.2

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    DOI： 10.1111/cas.13915

  • Development of PDMS Microchannel Integrated Type Terahertz Chip

    R. Taie, K. Serita, K. Kitagishi, T. Kawai, I. Kawayama, H. Murakami, M. Tonouchi

    International Conference on Infrared, Millimeter, and Terahertz Waves, IRMMW-THz   2018-September   2018.10

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    DOI： 10.1109/IRMMW-THz.2018.8510260

  • Profiling of N-linked glycans from 100 cells by capillary electrophoresis with large-volume dual preconcentration by isotachophoresis and stacking Reviewed

    Takayuki Kawai, Nobutoshi Ota, Akiko Imasato, Yoko Shirasaki, Koji Otsuka, Yo Tanaka

    Journal of Chromatography A   1565   138 - 144   2018.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.chroma.2018.06.034

  • Chiral Measurement of Aspartate and Glutamate in Single Neurons by Large-Volume Sample Stacking Capillary Electrophoresis Reviewed

    Amit V. Patel, Takayuki Kawai, Liping Wang, Stanislav S. Rubakhin, Jonathan V. Sweedler

    Analytical Chemistry   89 ( 22 )   12375 - 12382   2017.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/acs.analchem.7b03435

  • Combination of large-volume sample stacking with an electroosmotic flow pump with field-amplified sample injection on cross-channel chips Reviewed

    Fumihiko Kitagawa, Tatsuya Ishiguro, Misaki Tateyama, Isoshi Nukatsuka, Kenji Sueyoshi, Takayuki Kawai, Koji Otsuka

    Electrophoresis   38 ( 16 )   2075 - 2080   2017.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/elps.201700155

  • On-line coupling of sample preconcentration by LVSEP with gel electrophoretic separation on T-channel chips Reviewed

    Fumihiko Kitagawa, Saeko Kinami, Yuuki Takegawa, Isoshi Nukatsuka, Kenji Sueyoshi, Takayuki Kawai, Koji Otsuka

    Electrophoresis   38 ( 2 )   380 - 386   2017.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/elps.201600184

  • Micro/nanoparticle separation via curved nano-gap device with enhanced size resolution Reviewed

    Nobutoshi Ota, Yuri Owa, Takayuki Kawai, Yo Tanaka

    Journal of Chromatography A   1455   172 - 177   2016.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.chroma.2016.05.064

  • Free D-aspartate in nonmammalian animals: Detection, localization, metabolism, and function Reviewed

    Amit V. Patel, Takayuki Kawai, Stanislav S. Rubakhin, Jonathan V. Sweedler

    D-Amino Acids: Physiology, Metabolism, and Application   173 - 197   2016.1

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    Language：English  

    DOI： 10.1007/978-4-431-56077-7_12

  • オンライン試料濃縮法を利用した微量生体試料のCE分析 Invited Reviewed

    川井 隆之

    電気泳動   59 ( 2 )   91 - 93   2015.10

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    DOI： 10.2198/electroph.59.91

  • Simple valves on a pdms microchip bonded via patterned oxygen plasma Reviewed

    T. Kawai, H. Moriguchi, Y. Tanaka

    IEEE Transducers - 18th International Conference on Solid-State Sensors, Actuators and Microsystems   18   1782 - 1785   2015.8

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    Language：English   Publishing type：Research paper (other academic)  

    DOI： 10.1109/TRANSDUCERS.2015.7181292

  • Simple and effective label-free capillary electrophoretic analysis of sugars by complexation using quinoline boronic acids Reviewed

    Takuya Kubo, Koichi Kanemori, Risa Kusumoto, Takayuki Kawai, Kenji Sueyoshi, Toyohiro Naito, Koji Otsuka

    Analytical Chemistry   87 ( 10 )   5068 - 5073   2015.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/acs.analchem.5b00998

  • Hydrophilic interaction electrokinetic chromatography using bio-based nanofillers Reviewed

    Takayuki Kawai, Masato Watanabe, Kojiro Uetani, Yudai Fukushima, Kenji Sueyoshi, Takuya Kubo, Fumihiko Kitagawa, Hiroyuki Yano, Koji Otsuka

    Electrophoresis   35 ( 15 )   2229 - 2236   2014.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/elps.201300558

  • Toward 10000-fold sensitivity improvement of oligosaccharides in capillary electrophoresis using large-volume sample stacking with an electroosmotic flow pump combined with field-amplified sample injection Reviewed

    Takayuki Kawai, Masumi Ueda, Yudai Fukushima, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka

    Electrophoresis   34 ( 16 )   2303 - 2310   2013.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/elps.201200615

  • Rotatable reagent cartridge for high-performance microvalve system on a centrifugal microfluidic device Reviewed

    Takayuki Kawai, Nahoko Naruishi, Hidenori Nagai, Yoshihide Tanaka, Yoshihisa Hagihara, Yasukazu Yoshida

    Analytical Chemistry   85 ( 14 )   6587 - 6592   2013.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/ac400667e

  • Electrophoretic analysis of cations using large-volume sample stacking with an electroosmotic flow pump using capillaries coated with neutral and cationic polymers Reviewed

    Takayuki Kawai, Jun Ito, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka

    Journal of Chromatography A   1267   65 - 73   2012.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.chroma.2012.09.077

  • Highly sensitive chiral analysis in capillary electrophoresis with large-volume sample stacking with an electroosmotic flow pump Reviewed

    Takayuki Kawai, Hiroshi Koino, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka

    Journal of Chromatography A   1246   28 - 34   2012.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.chroma.2012.02.001

  • Highly sensitive oligosaccharide analysis in capillary electrophoresis using large-volume sample stacking with an electroosmotic flow pump Reviewed

    Takayuki Kawai, Masato Watanabe, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka

    Journal of Chromatography A   1232   52 - 58   2012.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.chroma.2011.09.032

  • Hydrophobic labeling of amino acids: Transient trapping-capillary/microchip electrophoresis Reviewed

    Kenji Sueyoshi, Kota Hashiba, Takayuki Kawai, Fumihiko Kitagawa, Koji Otsuka

    Electrophoresis   32 ( 10 )   1233 - 1240   2011.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/elps.201000567

  • Microchip electrophoresis of oligosaccharides using large-volume sample stacking with an electroosmotic flow pump in a single channel Reviewed

    Takayuki Kawai, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka

    Analytical Chemistry   82 ( 15 )   6504 - 6511   2010.8

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    Language：English   Publishing type：Research paper (other academic)  

    DOI： 10.1021/ac1008145

  • Muc4 is required for activation of ErbB2 in signet ring carcinoma cell lines Reviewed

    Atsushi Yokoyama, Bin-Hai Shi, Takayuki Kawai, Hiroaki Konishi, Ryota Andoh, Hiroyuki Tachikawa, Sayoko Ihara, Yasuhisa Fukui

    Biochemical and Biophysical Research Communications   355 ( 1 )   200 - 203   2007.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrc.2007.01.133

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Books

  • CE-MS Approaches for Single Cell Metabolomics

    Takayuki Kawai（ Role： Sole author）

    Wiley-VCH, Weinheim  2022.10 

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    Responsible for pages：Capillary Electrophoresis- Mass Spectrometry (CE-MS) for Proteomics and Metabolomics, 2022 (ISBN: 978-3-527-34921-0)   Language：English   Book type：Scholarly book

  • CE-MS Approaches for Single Cell Metabolomics in a Book "Capillary Electrophoresis - Mass Spectrometry for Proteomics and Metabolomics: Principles and Applications"

    川井 隆之

    Wiley  2022    （ ISBN:9783527349210 ）

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    Total pages：400  

    CiNii Research

  • Free d-Aspartate in Nonmammalian Animals: Detection, Localization, Metabolism, and Function

    Amit V. Patel, Takayuki Kawai, Stanislav S. Rubakhin, Jonathan V. Sweedler

    Springer  2016.8 

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    Language：Others  

    Free d-Aspartate in Nonmammalian Animals: Detection, Localization, Metabolism, and Function

  • 食のバイオ計測の最前線―機能解析と安全・安心の計測を目指して―

    北川 文彦, 川井 隆之, 大塚 浩二（ Role： Joint author）

    CMC出版  2011.5 

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    Responsible for pages：総ページ数：277   Language：Japanese  

Presentations

  • 超高感度糖鎖分析法の開発（仮題） Invited

    川井 隆之

    第47回 日本分子生物学会年会@福岡国際会議場  2024.11 

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    Event date： 2024.11

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：福岡国際会議場   Country：Japan  

  • サブセルラー質量分析技術が切り拓く生体膜研究 Invited

    川井 隆之

    日本膜学会第45年会・膜シンポジウム2023合同大会  2023.11 

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    Event date： 2023.11

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：早稲田大学リサーチイノベーションセンター   Country：Japan  

  • 超高感度CE-MS分析法による脳分子探査 Invited

    川井 隆之

    第96回日本生化学会大会  2023.11 

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    Event date： 2023.11

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：福岡国際会議場   Country：Japan  

  • Ultra-sensitive Bioanalysis by Dual-Stacking Capillary Electrophoresis Invited International conference

    Takayuki Kawai

    19th Asia-Pacific International Symposium on Microscale Separations and Analysis 2023 (APCE 2023)  2023.10 

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    Event date： 2023.10

    Language：English   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

  • 超高感度CE-MS分析技術に基づくドラッグデリバリー創薬支援事業 Invited

    川井 隆之

    九州大学発スタートアップマッチングセミナー  2023.10 

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    Event date： 2023.10

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：日本橋ライフサイエンスビル   Country：Japan  

  • 微量脳内分子の網羅解析に向けた超高感度分析技術の開発 Invited

    川井 隆之

    第96回 日本薬理学会年会  2022.12 

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    Event date： 2022.11 - 2022.12

    Language：Japanese  

    Country：Other  

  • 1細胞メタボロミクスへの挑戦 Invited

    川井 隆之

    第95回 日本生化学会大会  2022.11 

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    Event date： 2022.11

    Language：Japanese  

    Country：Other  

  • 超高感度CE-MS分析プラットフォームの開発と微量バイオ分析への応用 Invited

    川井 隆之

    第42回キャピラリー電気泳動シンポジウム  2022.10 

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    Event date： 2022.10

    Language：Japanese  

    Country：Other  

  • キャピラリー電気泳動に基づく超高感度N結合糖鎖分析法の開発と組織微小環境分析への応用 Invited

    川井 隆之

    第41回日本糖質学会年会  2022.9 

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    Event date： 2022.9 - 2022.10

    Language：Japanese  

    Country：Other  

  • キャピラリー電気泳動における技術開発から応用まで Invited

    川井 隆之

    第41回キャピラリー電気泳動シンポジウム  2021.12 

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    Event date： 2021.12

    Language：Japanese  

    Country：Other  

  • キャピラリー電気泳動を駆使した超高感度グライコーム分析法の開発と応用 Invited

    川井 隆之

    第18回JCGGシンポジウム  2021.12 

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    Event date： 2021.12

    Language：Japanese  

    Country：Other  

  • Development of Ultra-sensitive Capillary Electrophoresis and Application to in vitro/vivo Trace Bioanalysis Invited

    Takayuki Kawai

    Pittcon 2021  2021.3 

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    Event date： 2021.3

    Language：English  

    Country：Other  

    Development of Ultra-sensitive Capillary Electrophoresis and Application to in vitro/vivo Trace Bioanalysis

  • 極微量・定量的メタボローム分析技術の開発と応用 Invited

    川井 隆之

    日本質量分析学会 イオン反応研究部会 2020年前期研究会  2021.2 

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    Event date： 2021.2

    Language：Japanese  

    Country：Other  

  • 超高感度キャピラリー電気泳動法の開発と組織微小環境のオミックス分析 Invited

    川井 隆之

    日本分析化学会 第69年会  2020.9 

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    Event date： 2020.9

    Language：Japanese  

    Country：Other  

  • 超高感度キャピラリー電気泳動－質量分析法の開発と一細胞メタボローム分析への応用 Invited

    川井 隆之

    日本分析化学会 第80回分析化学討論会  2020.5 

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    Event date： 2020.5

    Language：Japanese  

    Country：Other  

  • Microchip Electrophoresis of Oligosaccharides with Large-volume Sample Stacking with Electroosmotic Flow Pump in Single Channel International conference

    Takayuki Kawai, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka

    2010 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2010)  2010.12 

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    Microchip Electrophoresis of Oligosaccharides with Large-volume Sample Stacking with Electroosmotic Flow Pump in Single Channel

  • Highly Sensitive Chiral Analysis in Capillary Electrophoresis Using Large-volume Sample Stacking with Electroosmotic Flow Pump International conference

    Takayuki Kawai, Jun Ito, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka

    26th International Symposium on Microscale Bioseparations (MSB 2011)  2011.5 

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    Highly Sensitive Chiral Analysis in Capillary Electrophoresis Using Large-volume Sample Stacking with Electroosmotic Flow Pump

  • Highly Sensitive Chiral Analysis by Capillary Electrophoresis Using Large-volume Sample Stacking with Electroosmotic Flow Pump International conference

    Takayuki Kawai, Jun Ito, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka

    IUPAC International Congress on Analytical Sciences 2011 (ICAS 2011)  2011.5 

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    Highly Sensitive Chiral Analysis by Capillary Electrophoresis Using Large-volume Sample Stacking with Electroosmotic Flow Pump

  • Toward 10,000-fold Sample Preconcentration Efficiency for Oligosaccharide Analysis in Capillary Electrophoresis International conference

    Takayuki Kawai, Masumi Ueda, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka

    37th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2011 Dalian)  2011.10 

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    Language：English  

    Country：Other  

    Toward 10,000-fold Sample Preconcentration Efficiency for Oligosaccharide Analysis in Capillary Electrophoresis

  • Toward 10,000-fold Sensitivity Improvement in Capillary Electrophoresis of Oligosaccharide International conference

    Takayuki Kawai, Masumi Ueda, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka

    11th Asia-Pacific International Symposium on Microscale Separations and Analysis (APCE 2011)  2011.11 

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    Language：English  

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    Toward 10,000-fold Sensitivity Improvement in Capillary Electrophoresis of Oligosaccharide

  • Analysis of Enantiomeric Amino Acids in Biological Samples via Capillary Electrophoresis Coupled with Laser-induced Fluorescence and Mass Spectrometry International conference

    Takayuki Kawai, Nobutoshi Ota, Jonathan V. Sweedler

    Pittcon 2014  2014.3 

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    Language：English  

    Country：Other  

    Analysis of Enantiomeric Amino Acids in Biological Samples via Capillary Electrophoresis Coupled with Laser-induced Fluorescence and Mass Spectrometry

  • Simple and High-Performance On-chip Bioanalysis Invited International conference

    Takayuki Kawai

    EMN Summer Meeting 2014  2014.6 

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    Simple and High-Performance On-chip Bioanalysis

  • Analysis of Enantiomeric Amino Acids in Biological Samples via Capillary Electrophoresis Coupled with Mass Spectrometry International conference

    Takayuki Kawai, Stanislav S. Rubakhin, Jonathan V. Sweedler

    AS-MS 2014  2014.6 

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    Analysis of Enantiomeric Amino Acids in Biological Samples via Capillary Electrophoresis Coupled with Mass Spectrometry

  • Analysis of D-Glutamate in a Single Cell via Capillary Electrophoresis Coupled with an Online Sample Preconcentration Method International conference

    Takayuki Kawai, Amit Patel, Stanislav S Rubakhin, Jonathan V Sweedler

    The 2nd International Conference of D-Amino Acid Research (IDAR 2014)  2014.9 

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    Language：English  

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    Analysis of D-Glutamate in a Single Cell via Capillary Electrophoresis Coupled with an Online Sample Preconcentration Method

  • Sensitive and Flexible Single-Cell Analysis via Capillary Electrophoresis Coupled with an Online Sample Preconcentration Method Invited International conference

    Takayuki Kawai

    1st Caparica Christmas Conference on Sample Treatment (Sample Treatment 2014)  2014.12 

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    Sensitive and Flexible Single-Cell Analysis via Capillary Electrophoresis Coupled with an Online Sample Preconcentration Method

  • Ultra-sensitive capillary electrophoresis for single cell analysis Invited International conference

    Takayuki Kawai

    International Conference and Expo on Separation Techniques 2015  2015.8 

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    Ultra-sensitive capillary electrophoresis for single cell analysis

  • オンライン試料濃縮法を利用した微量生体試料のCE分析 Invited

    川井 隆之

    第66回日本電気泳動学会総会  2015.9 

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  • キャピラリー電気泳動を用いた高感度一細胞分析法の開発 Invited

    川井 隆之

    第35回キャピラリー電気泳動シンポジウム  2015.11 

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    Language：Japanese  

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  • ミクロスケール空間を利用した高性能バイオ分析法の開発 Invited

    川井 隆之

    2015年度 上智大学物質生命理工学科コロキウム  2016.1 

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  • High performance CE-MS system for single cell analysis Invited International conference

    Takayuki Kawai

    32nd International Symposium on Microscale Separations and Bioanalysis (MSB 2016)  2016.4 

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    High performance CE-MS system for single cell analysis

  • Ultra-sensitive CE-LIF/MS System for Single Cell Omics Research Invited International conference

    Takayuki Kawai

    16th Asia-Pacific International Symposium on Microscale Separations and Analysis (APCE2016)  2016.11 

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    Ultra-sensitive CE-LIF/MS System for Single Cell Omics Research

  • 超高感度CE-MS分析システムによる極微量オミックス解析 Invited

    川井 隆之

    日本化学会 第97春季年会  2017.3 

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  • Ultra-sensitive Capillary Electrophoresis for Single Cell Omics Research Invited International conference

    Takayuki Kawai

    Pittcon 2017  2017.3 

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    Country：Other  

    Ultra-sensitive Capillary Electrophoresis for Single Cell Omics Research

  • Quantitative Single Cell Metabolomics by CE-MS Invited International conference

    Takayuki Kawai

    17th Asia-Pacific International Symposium on Microscale Separations and Analysis (APCE2017)  2017.11 

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    Language：English  

    Country：Other  

    Quantitative Single Cell Metabolomics by CE-MS

  • 超高感度CE-MSを用いた一細胞メタボローム・プロテオーム分析 Invited

    川井 隆之

    第6回バイオ関連シンポジウム 若手フォーラム  2018.9 

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  • 微量質量分析法の開発と応用 Invited

    川井 隆之

    東北大学薬学部セミナー  2018.11 

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  • キャピラリー電気泳動を用いた微量生体試料分析 Invited

    川井 隆之

    第38回 キャピラリー電気泳動シンポジウム  2018.12 

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  • 超高感度キャピラリー電気泳動を用いた微量糖鎖プロファイリング法 Invited

    川井 隆之

    理研シンポジウム「細胞内糖修飾の統合的ケミカルバイオロジー」  2019.1 

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  • 高感度キャピラリー電気泳動システムを用いた微量生体試料分析 Invited

    川井 隆之

    分析化学会近畿支部講演会  2019.7 

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  • 超高感度キャピラリー電気泳動を用いた微量オミックス分析 Invited

    川井 隆之

    日本プロテオーム学会2019年大会・第70回日本電気泳動学会総会 合同大会  2019.7 

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  • キャピラリー電気泳動の高感度化と微量生体試料分析への応用 Invited

    川井 隆之

    2019年度日本分析化学会近畿支部ぶんせき講習会 (実践編)  2019.7 

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  • Development of Ultra-sensitive Capillary Electrophoresis toward Trace N-glycan Profiling Invited

    Takayuki Kawai

    Asian Glyco Webinar  2022.4 

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    Development of Ultra-sensitive Capillary Electrophoresis toward Trace N-glycan Profiling

  • オンライン試料濃縮－キャピラリー電気泳動による超高感度・微量バイオ分析 Invited

    川井 隆之

    大塚電子Webセミナー  2022.12 

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  • LC/CE二次元分離に基づく超高感度・絶対定量グライコーム解析法の開発 Invited

    川井 隆之

    第74回日本電気泳動学会学術大会  2023.5 

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MISC

  • 超高感度質量分析技術が切り拓くミクロスケール薬物動態研究 Reviewed

    川井 隆之

    膜   2024.3

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  • 超高感度キャピラリー電気泳動による微量バイオ分析法の開発

    川井 隆之

    分析化学   72 ( 9 )   349 - 355   2023.9   （ ISSN:0525-1931 ）

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    Language：Japanese   Publisher：(公社)日本分析化学会  

    キャピラリー電気泳動(CE)は高効率な分離法であり,レーザー励起蛍光や質量分析などの高感度検出法と組み合わせることで,nLオーダーの試料溶液に含まれるzmolレベルの生体分子を検出できる.しかし一般的な生体試料の分析では,前処理においてμLオーダーの溶液を調製する必要があり,nLオーダーの試料しか注入できないCEではほとんどの溶液を無駄にしてしまい,CEの分析性能を活かすことができない.そこで著者は,CEにおいてμLオーダーの大量の試料を導入できる新規二重オンライン試料濃縮法を開発し,生体分子や薬剤などを対象に1000倍前後の濃縮を実現してきた.これらの濃縮法を用いることで,1～100細胞程度の培養細胞や組織切片に含まれる糖鎖・代謝物・薬剤などを分析することに成功した.本論文ではこれらの研究成果について紹介する.(著者抄録)

  • マイクロ流路残留圧力を利用したゲルキャスティング

    田中信行, 森口裕之, 佐藤麻子, 川井隆之, 田中陽

    化学とマイクロ・ナノシステム学会研究会講演要旨集   2015.11

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  • 酸素プラズマパターニングを利用したシンプルなPDMSバルブの開発

    森口裕之, 川井隆之, 神田元紀, 山田陸裕, 上田泰己, 田中陽

    化学とマイクロ・ナノシステム学会研究会講演要旨集   2014.10

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    Language：Japanese  

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Utility model   Number of applications: 0   Number of registrations: 0
Design   Number of applications: 0   Number of registrations: 0
Trademark   Number of applications: 0   Number of registrations: 0

Professional Memberships

  • SOCIETY FOR CHROMATOGRAPHIC SCIENCES

  • THE SOCIETY FOR CHEMISTRY AND MICRO-NANO SYSTEMS

  • THE JAPAN SOCIETY FOR ANALYTICAL CHEMISTRY

  • THE CHEMICAL SOCIETY OF JAPAN

  • JAPANESE ELECTROPHORESIS SOCIETY

  • The Japanese Society of Carbohydrate Research

  • The Japanese Biochemical Society

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Committee Memberships

  • 日本分析化学会 近畿支部   常任幹事   Domestic

    2020.4 - 2020.12   

  • Councilor   Domestic

    2019.7 - Present   

  • Organizer   Domestic

    2015.4 - 2020.3   

Academic Activities

  • 世話人 (実行委員長)

    第74回日本電気泳動学会シンポジウム  2024.9

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    Type：Competition, symposium, etc. 

  • Analytical Sciences International contribution

    2022.3 - 2026.3

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    Type：Academic society, research group, etc. 

  • 実行委員

    第42回キャピラリー電気泳動シンポジウム  （ 福岡 ） 2021.12

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    Type：Competition, symposium, etc. 

Research Projects

  • 臨床検査応用に向けた超高感度CE-MSメタボローム分析法の開発

    2024

    日本医療研究開発機構 (AMED) 橋渡し研究プログラム シーズH

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    Authorship：Principal investigator  Grant type：Contract research

  • 超高感度キャピラリー電気泳動技術を基盤とした次世代グライコシーケンサーの開発

    Grant number：23H02623  2023 - 2026

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • 超高感度 CE-MS 薬物動態解析に基づくドラッグデリバリー創薬支援事業

    2023

    九州大学 ギャップファンド

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    Authorship：Principal investigator  Grant type：On-campus funds, funds, etc.

  • 生体膜ナノ構造の可視化と脂質網羅分析のための統合ナノ分析システム

    Grant number：22K18950  2022 - 2023

    日本学術振興会  科学研究費助成事業  挑戦的研究（萌芽）

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • 超高感度キャピラリー電気泳動法を用いた受託分析サービスとその多角展開

    2022

    日本科学技術振興機構 (JST) 研究成果展開事業 大学発新産業創出プログラム (START) 大学・エコシステム推進型 スタートアップ・エコシステム形成支援 (PARKS)

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    Authorship：Principal investigator  Grant type：Contract research

  • 超高感度CE-MS技術に基づくミクロスケール薬物動態評価プラットフォーム

    2021 - 2025

    日本医療研究開発機構 (AMED) 創薬基盤推進研究事業

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    Authorship：Principal investigator  Grant type：Contract research

  • 微量脳内分子の完全網羅解析を実現する極限検出システムの開発

    Grant number：21H05092  2021 - 2023

    日本学術振興会・文部科学省  科学研究費助成事業  学術変革領域研究(B)

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • 革新的ナノテクノロジーによる脳分子探査

    Grant number：21H05089  2021 - 2023

    日本学術振興会・文部科学省  科学研究費助成事業  学術変革領域研究(B)

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    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

  • 分子脳科学研究を加速する革新的技術基盤の開発

    2021 - 2023

    科学技術振興機構 (JST) 未来社会創造事業

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    Authorship：Coinvestigator(s)  Grant type：Contract research

  • 超高感度・絶対定量グライコーム解析法の開発および組織微小環境における糖鎖修飾恒常性の理解と医療応用

    2020 - 2023

    日本医療研究開発機構 (AMED) 革新的先端研究開発支援事業 (PRIME)

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    Authorship：Principal investigator  Grant type：Contract research

  • ナノスケール電気泳動法による一分子グライコーム解析

    Grant number：19H02567  2019 - 2022

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • １分子スケール蛍光分析化学の創出

    Grant number：19H05545  2019 - 2022

    日本学術振興会  科学研究費助成事業  挑戦的研究（開拓）

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    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

  • 微小流路デバイスを用いた分析における高分解能・高感度テラヘルツ分光検出法の確立

    Grant number：17H03252  2017 - 2019

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

  • 超高効率濃縮法に基づくCE-LIF-MS微量糖鎖分析システムの開発

    2016 - 2020

    日本医療研究開発機構 (AMED) 糖鎖利用による革新的創薬技術開発事業

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    Authorship：Principal investigator  Grant type：Contract research

  • フグ肝毒性分析チップの開発を通じた新次元食品科学分野の開拓

    2016 - 2019

    科学研究費助成事業  挑戦的萌芽研究

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • 超高感度CE-MS分析システムによる極微量プロテオーム解析

    2014 - 2017

    戦略的創造研究推進事業 (文部科学省)

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    Authorship：Principal investigator  Grant type：Contract research

  • 迅速プロテオミクス分析のための並列化CE-MSシステムの開発

    Grant number：26888021  2014 - 2015

    日本学術振興会  科学研究費助成事業  研究活動スタート支援

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • バイオマスナノ材料との親水性相互作用を利用した高性能バイオ分析システムの開発

    2013 - 2014

    日本学術振興会  科学研究費助成事業  特別研究員奨励費

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • PEG化リン脂質ミセルによる糖鎖電気泳動分析の高性能化

    2009 - 2011

    日本学術振興会  科学研究費助成事業  特別研究員奨励費

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    Authorship：Principal investigator  Grant type：Scientific research funding

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Educational Activities

  • Lecture and practice in both graduate undergraduate schools. Special research education in the laboratory of bioanalytical chemistry. Join some committees in the university.

Class subject

  • 自然科学総合実験

    2024.10 - 2025.3   Second semester

  • 分析化学実験

    2024.10 - 2025.3   Second semester

  • 分析化学I

    2024.4 - 2024.9   First semester

  • 国際科学特論III

    2024.4 - 2024.9   First semester

  • 分析化学実験

    2023.10 - 2024.3   Second semester

  • 自然科学総合実験

    2023.10 - 2024.3   Second semester

  • 分析化学I

    2023.4 - 2023.9   First semester

  • 分析化学特論IIB

    2023.4 - 2023.9   First semester

  • 国際科学特論III

    2023.4 - 2023.9   First semester

  • 自然科学総合実験

    2022.10 - 2023.3   Second semester

  • 分析化学実験

    2022.10 - 2023.3   Second semester

  • 国際科学特論III

    2022.4 - 2022.9   First semester

  • 分析化学実験

    2021.10 - 2022.3   Second semester

  • 自然科学総合実験

    2021.10 - 2022.3   Second semester

  • 基礎化学実習

    2021.10 - 2022.3   Second semester

  • 化学序説 (R2年度以前入学者用)

    2021.4 - 2021.9   First semester

  • 化学序説 (R3年度入学者用)

    2021.4 - 2021.9   First semester

  • 分析化学特論IIB

    2021.4 - 2021.9   First semester

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