Updated on 2024/11/29

Information

 

写真a

 
HIRAMATSU KOTARO
 
Organization
Faculty of Science Department of Chemistry Associate Professor
School of Sciences Department of Chemistry(Concurrent)
Graduate School of Sciences Department of Chemistry(Concurrent)
Title
Associate Professor
Contact information
メールアドレス
Tel
0928024189

Degree

  • Ph.D.

Research History

  • Kyushu University Faculty of Sciences Department of Chemistry Associate Professor 

    2023.10 - Present

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    Country:Japan

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  • 2011年3月 東京大学理学部化学科卒業 2013年3月 東京大学大学院理学系研究科化学専攻 修士課程修了 2013年4月-2016年3月 日本学術振興会特別研究員DC1 2016年3月 東京大学大学院理学系研究科化学専攻 博士課程修了 2016年3月 東京大学フォトンサイエンスリーディング大学院 修了 2016年4月 - 2016年8月 理化学研究所田原分子分光研究室 基礎科学特別研究員 2016年9月 - 現在 東京大学大学院理学系研究科化学専攻 スペクトル化学研究センター 助教 2018年10月 – 2022年3月 国立研究開発法人科学技術振興機構 さきがけ研究員   

Research Interests・Research Keywords

  • Research theme: Molecular spectroscopy

    Keyword: Molecular spectroscopy

    Research period: 2024

  • Research theme: Raman optical activity

    Keyword: Raman optical activity

    Research period: 2024

  • Research theme: We are engaged in the development of methods to efficiently explore unexplored temporal, spatial, and energy domains by integrating optical measurement and information processing. Specifically, we are developing methodologies for the creation of new useful strains by combining large-scale cell measurement and sorting with genetic mutation, and for providing feedback to material design guidelines by elucidating the material destruction process through high-speed and long-term measurement of spectra. By maximizing the potential of light, we are working to discover new phenomena and elucidate their mechanisms by creating a chemical eye that makes the previously invisible visible.

    Keyword: Molecular spectroscopy, laser engineering, vibrational spectroscopy, superresolution microscopy, bioimaging, ultrafast spectroscopy

    Research period: 2023.10

Awards

  • 日本分光学会奨励賞

    2024.6   日本分光学会   ラマン分光フローサイトメトリーの開発と大規模細胞解析

  • 日本分光学会奨励賞

    2024.6   日本分光学会   ラマン分光フローサイトメトリーの開発と大規模細胞解析

    平松 光太郎

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  • コニカミノルタ画像科学奨励賞 連携賞

    2024.2   コニカミノルタ科学技術振興財団   高速超解像イメージングのための空間―周波数圧縮顕微鏡の開発

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    2014年にノーベル化学賞を受賞したBetzig, Moernerらによって開発されたSingle-molecule localization microscopy (SMLM)は,通常の光学顕微鏡の10倍以上の空間分解能を有し,細胞内の個別のタンパク質の局在を可視化することができる.しかしながら,SMLMでは1つの超解像画像を取得するのに,蛍光を明滅させながら数千回から数万回の撮像(1回の撮像を30ミリ秒としても,数十秒から数分を要する)を行う必要があり,細胞内分子の動態を計測することは困難である.本研究では,明滅の代わりに焦点内の各プローブ分子からの信号を分光学的に分離することで、シングルショットSMLM撮像を実現する.具体的には,空間―周波数圧縮ラマン顕微鏡(光と画像に関するシステム)とスペクトルの線形独立性と空間的なスパース性を用いたデータ解析法(光と画像に関するソフトウェア)を開発・融合することによりプローブ分子の位置を超精度で決定できるSMARTS: Superresolution Microscopy with Advanced Raman Tags and Sparse modelingを創出する.それにより超解像計測の時間分解能を飛躍的に向上させ,細胞内輸送などの時空間的に複雑な構造をもつ生命現象の原理解明に向けた技術基盤を確立することを目指す.

  • コニカミノルタ画像科学奨励賞 連携賞

    2024.2   コニカミノルタ科学技術振興財団   高速超解像イメージングのための空間―周波数圧縮顕微鏡の開発

    平松 光太郎, 小野 峻佑

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  • 光学論文賞

    2023.11   日本光学会   Ultrafast, dual-band coherent Raman spectroscopy without ultrashort pulses

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    コヒーレントラマン分光法は、高速かつ無標識に分子レベルの構造情報を得られる手法として近年注目を集めている。コヒーレントラマン分光法と顕微鏡を組み合わせた振動分光イメージングや、コヒーレントラマン分光法とフローサイトメトリーを組み合わせた大規模細胞解析法などがこれまでに実証されてきた。応募者らは超短パルスレーザーを用いて時間領域で分子振動を励起・検出するFourier-transform coherent Raman scattering (FT-CARS)法を用いて、高速(24,000 spectra/s)かつ広帯域(200 – 1600 cm-1)な振動分光計測を実現するとともに、様々な対象へとその応用を実証してきた。しかしながら、周波数領域で計測を行うCARS分光法やStimulated Raman scattering (SRS)法などと比較したときに、FT-CARS法では、CH伸縮振動が観測される高波数領域(2800 – 3400 cm-1)が計測できないといった欠点があった。

    本研究では、これまでに開発してきたFT-CARS法に用いているチタンサファイアレーザーに同期して発振するYbファイバーレーザー(YbF)を併せて光源として用いることで、FT-CARS法でも高波数領域の計測が可能であることを実証した。これまでのFT-CARS計測では、パルス幅15 fs程度のチタンサファイアレーザー(TiS)からの出力(750 – 850 nm)をpump光とprobe光へと分割し、それらを様々なパルス間隔でサンプルへと照射することで、分子振動の時間発展を計測していた。今回開発した手法では、従来のFT-CARS分光計をベースとして、YbFからの出力(~1030 nm)を同時に照射することで、CH伸縮領域の計測を可能とした。TiSの出力の一部をYbFのキャビティ内へと導入し、相互位相変調によってYbFキャビティ内で2つのパルスが同じ群速度で伝搬しYbFとTiSの高精度な同期発振を実現した。

  • 光学論文賞

    2023.11   日本光学会   Ultrafast, dual-band coherent Raman spectroscopy without ultrashort pulses

    平松 光太郎

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  • 日本分光学会年次講演会若手講演賞

    2021.11  

  • 研究開発奨励賞 エヌエフホールディングス特別賞

    2020.12   エヌエフ基金   Encouraging prize for research and development

  • 優秀講演賞(学術)

    2019.4   日本化学会   Presentation Award (Academic)

  • 第47回(2019年秋季)応用物理学会講演奨励賞

    2019.3  

  • Best Presentation Award, SPIE Photonics West

    2019.2   SPIE   Best Presentation Award, SPIE Photonics West

  • 理学系研究科研究奨励賞

    2016.3   東京大学   School of Science Research Award

  • Springer Thesis Award

    2016.3   Springer Thesis Award

  • 第9回分子科学討論会 優秀講演賞

    2015.10   Best Presentation Award, 9th Annual Meeting of Japan Society for Molecular Science

  • Poster Award, 15th International Conference on Chiroptical Spectroscopy (CD2015)

    2015.9   Poster Award, 15th International Conference on Chiroptical Spectroscopy (CD2015)

  • 日本分光学会年次講演会若手講演賞

    2015.6   日本分光学会   Best Presentation Award, Annual Meeting of Spectroscopical Society of Japan 2015

  • 日本化学会 第95春季年会 (2015)学生講演賞

    2015.3   CSJ Student Presentation Award 2015

  • 第6回分子科学討論会優秀講演賞

    2012.9   Best Presentation Award, 6th Annual Meeting of Japan Society for Molecular Science

  • Best Poster Award, 3rd Asian Spectroscopy Conference

    2011.12   Best Poster Award, 3rd Asian Spectroscopy Conference

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Papers

  • High-throughput fluorescence lifetime imaging flow cytometry. International journal

    Hiroshi Kanno, Kotaro Hiramatsu, Hideharu Mikami, Atsushi Nakayashiki, Shota Yamashita, Arata Nagai, Kohki Okabe, Fan Li, Fei Yin, Keita Tominaga, Omer Faruk Bicer, Ryohei Noma, Bahareh Kiani, Olga Efa, Martin Büscher, Tetsuichi Wazawa, Masahiro Sonoshita, Hirofumi Shintaku, Takeharu Nagai, Sigurd Braun, Jessica P Houston, Sherif Rashad, Kuniyasu Niizuma, Keisuke Goda

    Nature communications   15 ( 1 )   7376 - 7376   2024.9

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    Flow cytometry is a vital tool in biomedical research and laboratory medicine. However, its accuracy is often compromised by undesired fluctuations in fluorescence intensity. While fluorescence lifetime imaging microscopy (FLIM) bypasses this challenge as fluorescence lifetime remains unaffected by such fluctuations, the full integration of FLIM into flow cytometry has yet to be demonstrated due to speed limitations. Here we overcome the speed limitations in FLIM, thereby enabling high-throughput FLIM flow cytometry at a high rate of over 10,000 cells per second. This is made possible by using dual intensity-modulated continuous-wave beam arrays with complementary modulation frequency pairs for fluorophore excitation and acquiring fluorescence lifetime images of rapidly flowing cells. Moreover, our FLIM system distinguishes subpopulations in male rat glioma and captures dynamic changes in the cell nucleus induced by an anti-cancer drug. FLIM flow cytometry significantly enhances cellular analysis capabilities, providing detailed insights into cellular functions, interactions, and environments.

    DOI: 10.1038/s41467-024-51125-y

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  • Fluorescence-Encoded Time-Domain Coherent Raman Spectroscopy in the Visible Range

    Tetsu Tamura, Phillip C. McCann, Ryo Nishiyama, Kotaro Hiramatsu, Keisuke Goda

    The Journal of Physical Chemistry Letters   15 ( 18 )   4940 - 4947   2024.4   ISSN:1948-7185

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    Fluorescence-encoded vibrational spectroscopy has attracted increasing attention by virtue of its high sensitivity and high chemical specificity. We recently demonstrated fluorescence-encoded time-domain coherent Raman spectroscopy (FLETCHERS), which enables low-frequency vibrational spectroscopy of low-concentration fluorophores using near-infrared (800-900 nm) light excitation. However, the feasibility of this study was constrained by the scarcity of excitable molecules in the near-infrared range. Consequently, the broader applicability of FLETCHERS has not been investigated. Here we extend the capabilities of FLETCHERS into the visible range by employing a noncollinear optical parametric amplifier as a light source, significantly enhancing its versatility. Specifically, we use the method, which we refer to as visible FLETCHERS (vFLETCHERS), to individually acquire Raman spectra from five visible fluorophores that have absorption peaks in the 600-700 nm region. These results not only confirm the versatility of vFLETCHERS for a wide range of molecules but also allude to its widespread applicability in biological research through highly sensitive supermultiplexed imaging.

    DOI: 10.1021/acs.jpclett.4c00817

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  • The marriage of coherent Raman scattering imaging and advanced computational tools

    Walker Peterson, Kotaro Hiramatsu, Keisuke Goda

    Light: Science and Applications   12 ( 1 )   2023.12   ISSN:2095-5545 eISSN:2047-7538

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    Coherent Raman scattering microscopy can provide high-contrast tissue and single-cell images based on the inherent molecular vibrations of the sample. However, conventional techniques face a three-way trade-off between Raman spectral bandwidth, imaging speed, and image fidelity. Although currently challenging to address via optical design, this trade-off can be overcome via emerging computational tools such as compressive sensing and machine learning.

    DOI: 10.1038/s41377-023-01160-z

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  • Boosting the Brightness of Raman Tags Using Cyanostar Macrocycles

    Ryo Nishiyama, Kei Furuya, Phillip McCann, Laura Kacenauskaite, Bo W. Laursen, Amar H. Flood, Kotaro Hiramatsu, Keisuke Goda

    Analytical Chemistry   95 ( 34 )   12835 - 12841   2023.8   ISSN:0003-2700 eISSN:1520-6882

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    DOI: 10.1021/acs.analchem.3c01958

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  • Label-free live microalgal starch screening via Raman flow cytometry

    Julia Gala de Pablo, Matthew Lindley, Kotaro Hiramatsu, Akihiro Isozaki, Keisuke Goda

    Algal Research   2023.3

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    DOI: 10.1016/j.algal.2023.102993

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  • High-speed hyperspectral imaging enabled by compressed sensing in time domain

    Shigekazu Takizawa, Kotaro Hiramatsu, Matthew Lindley, Julia Gala de Pablo, Shunsuke Ono, Keisuke Goda

    Advanced Photonics Nexus   2 ( 02 )   2023.3   ISSN:2791-1519

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    DOI: 10.1117/1.apn.2.2.026008

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  • Place & Play SERS: sample collection and preparation-free surface-enhanced Raman spectroscopy

    Yasutaka Kitahama, Pablo Martinez Pancorbo, Hiroki Segawa, Machiko Marumi, Ting-Hui Xiao, Kotaro Hiramatsu, William Yang, Keisuke Goda

    ANALYTICAL METHODS   15 ( 8 )   1028 - 1036   2023.2   ISSN:1759-9660 eISSN:1759-9679

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    The ability to perform sensitive, real-time, in situ, multiplex chemical analysis is indispensable for diverse applications such as human health monitoring, food safety testing, forensic analysis, environmental sensing, and homeland security. Surface-enhanced Raman spectroscopy (SERS) is an effective tool to offer the ability by virtue of its high sensitivity and rapid label-free signal detection as well as the availability of portable Raman spectrometers. Unfortunately, the practical utility of SERS is limited because it generally requires sample collection and preparation, namely, collecting a sample from an object of interest and placing the sample on top of a SERS substrate to perform a SERS measurement. In fact, not all analytes can satisfy this requirement because the sample collection and preparation process may be undesirable, laborious, difficult, dangerous, costly, or time-consuming. Here we introduce "Place & Play SERS" based on an ultrathin, flexible, stretchable, adhesive, biointegratable gold-deposited polyvinyl alcohol (PVA) nanomesh substrate that enables placing the substrate on top of an object of interest and performing a SERS measurement of the object by epi-excitation without the need for touching, destroying, and sampling it. Specifically, we characterized the sensitivity of the gold/PVA nanomesh substrate in the Place & Play SERS measurement scheme and then used the scheme to conduct SERS measurements of both wet and dry objects under nearly real-world conditions. To show the practical utility of Place & Play SERS, we demonstrated two examples of its application: food safety testing and forensic analysis. Our results firmly verified the new measurement scheme of SERS and are expected to extend the potential of SERS by opening up untapped applications of sensitive, real-time, in situ multiplex chemical analysis.

    DOI: 10.1039/d2ay02090d

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  • Color-scalable flow cytometry with Raman tags. International journal

    Ryo Nishiyama, Kotaro Hiramatsu, Shintaro Kawamura, Kosuke Dodo, Kei Furuya, Julia Gala de Pablo, Shigekazu Takizawa, Wei Min, Mikiko Sodeoka, Keisuke Goda

    PNAS nexus   2 ( 2 )   pgad001   2023.2

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    Flow cytometry is an indispensable tool in biology and medicine for counting and analyzing cells in large heterogeneous populations. It identifies multiple characteristics of every single cell, typically via fluorescent probes that specifically bind to target molecules on the cell surface or within the cell. However, flow cytometry has a critical limitation: the color barrier. The number of chemical traits that can be simultaneously resolved is typically limited to several due to the spectral overlap between fluorescence signals from different fluorescent probes. Here, we present color-scalable flow cytometry based on coherent Raman flow cytometry with Raman tags to break the color barrier. This is made possible by combining a broadband Fourier-transform coherent anti-Stokes Raman scattering (FT-CARS) flow cytometer, resonance-enhanced cyanine-based Raman tags, and Raman-active dots (Rdots). Specifically, we synthesized 20 cyanine-based Raman tags whose Raman spectra are linearly independent in the fingerprint region (400 to 1,600 cm-1). For highly sensitive detection, we produced Rdots composed of 12 different Raman tags in polymer nanoparticles whose detection limit was as low as 12 nM for a short FT-CARS signal integration time of 420 µs. We performed multiplex flow cytometry of MCF-7 breast cancer cells stained by 12 different Rdots with a high classification accuracy of 98%. Moreover, we demonstrated a large-scale time-course analysis of endocytosis via the multiplex Raman flow cytometer. Our method can theoretically achieve flow cytometry of live cells with >140 colors based on a single excitation laser and a single detector without increasing instrument size, cost, or complexity.

    DOI: 10.1093/pnasnexus/pgad001

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  • Label-free multiphoton imaging flow cytometry. International journal

    Ryo Kinegawa, Julia Gala de Pablo, Yi Wang, Kotaro Hiramatsu, Keisuke Goda

    Cytometry. Part A : the journal of the International Society for Analytical Cytology   2023.2

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    Label-free imaging flow cytometry is a powerful tool for biological and medical research as it overcomes technical challenges in conventional fluorescence-based imaging flow cytometry that predominantly relies on fluorescent labeling. To date, two distinct types of label-free imaging flow cytometry have been developed, namely optofluidic time-stretch quantitative phase imaging flow cytometry and stimulated Raman scattering (SRS) imaging flow cytometry. Unfortunately, these two methods are incapable of probing some important molecules such as starch and collagen. Here, we present another type of label-free imaging flow cytometry, namely multiphoton imaging flow cytometry, for visualizing starch and collagen in live cells with high throughput. Our multiphoton imaging flow cytometer is based on nonlinear optical imaging whose image contrast is provided by two optical nonlinear effects: four-wave mixing (FWM) and second-harmonic generation (SHG). It is composed of a microfluidic chip with an acoustic focuser, a lab-made laser scanning SHG-FWM microscope, and a high-speed image acquisition circuit to simultaneously acquire FWM and SHG images of flowing cells. As a result, it acquires FWM and SHG images (100 × 100 pixels) with a spatial resolution of 500 nm and a field of view of 50 μm × 50 μm at a high event rate of four to five events per second, corresponding to a high throughput of 560-700 kb/s, where the event is defined by the passage of a cell or a cell-like particle. To show the utility of our multiphoton imaging flow cytometer, we used it to characterize Chromochloris zofingiensis (NIES-2175), a unicellular green alga that has recently attracted attention from the industrial sector for its ability to efficiently produce valuable materials for bioplastics, food, and biofuel. Our statistical image analysis found that starch was distributed at the center of the cells at the early cell cycle stage and became delocalized at the later stage. Multiphoton imaging flow cytometry is expected to be an effective tool for statistical high-content studies of biological functions and optimizing the evolution of highly productive cell strains.

    DOI: 10.1002/cyto.a.24723

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  • Ultrafast, Dual-Band Coherent Raman Spectroscopy without Ultrashort Pulses

    Kotaro Hiramatsu, Tatsuya Tajima, Keisuke Goda

    ACS Photonics   9 ( 11 )   3522 - 3528   2022.10   ISSN:2330-4022 eISSN:2330-4022

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    DOI: 10.1021/acsphotonics.2c00768

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  • Highly Scalable, Wearable Surface-Enhanced Raman Spectroscopy

    Limei Liu, Pablo Martinez Pancorbo, Ting Hui Xiao, Saya Noguchi, Machiko Marumi, Hiroki Segawa, Siddhant Karhadkar, Julia Gala de Pablo, Kotaro Hiramatsu, Yasutaka Kitahama, Tamitake Itoh, Junle Qu, Kuniharu Takei, Keisuke Goda

    Advanced Optical Materials   10 ( 17 )   2022.9   eISSN:2195-1071

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    The last two decades have witnessed a dramatic growth of wearable sensor technology, mainly represented by flexible, stretchable, on-skin electronic sensors that provide rich information of the wearer's health conditions and surroundings. A recent breakthrough in the field is the development of wearable chemical sensors based on surface-enhanced Raman spectroscopy (SERS) that can detect molecular fingerprints universally, sensitively, and noninvasively. However, while their sensing properties are excellent, these sensors are not scalable for widespread use beyond small-scale human health monitoring due to their cumbersome fabrication process and limited multifunctional sensing capabilities. Here, a highly scalable, wearable SERS sensor is demonstrated based on an easy-to-fabricate, low-cost, ultrathin, flexible, stretchable, adhesive, and biointegratable gold nanomesh. It can be fabricated in any shape and worn on virtually any surface for label-free, large-scale, in situ sensing of diverse analytes from low to high concentrations (10–106 × 10−9 m). To show the practical utility of the wearable SERS sensor, the sensor is tested for the detection of sweat biomarkers, drugs of abuse, and microplastics. This wearable SERS sensor represents a significant step toward the generalizability and practicality of wearable sensing technology.

    DOI: 10.1002/adom.202200054

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  • Intelligent sort‐timing prediction for image‐activated cell sorting

    Yaqi Zhao, Akihiro Isozaki, Maik Herbig, Mika Hayashi, Kotaro Hiramatsu, Sota Yamazaki, Naoko Kondo, Shinsuke Ohnuki, Yoshikazu Ohya, Nao Nitta, Keisuke Goda

    Cytometry Part A   2022.6   ISSN:1552-4922 eISSN:1552-4930

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    DOI: 10.1002/cyto.a.24664

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/cyto.a.24664

  • High‐Throughput Raman‐Activated Cell Sorting in the Fingerprint Region

    Matthew Lindley, Julia Gala de Pablo, Walker Peterson, Akihiro Isozaki, Kotaro Hiramatsu, Keisuke Goda

    Advanced Materials Technologies   2101567 - 2101567   2022.4   ISSN:2365-709X eISSN:2365-709X

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    DOI: 10.1002/admt.202101567

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/admt.202101567

  • Ultrafast impulsive Raman spectroscopy across the terahertz-fingerprint region

    Walker Peterson, Julia Gala De Pablo, Matthew Lindley, Kotaro Hiramatsu, Keisuke Goda

    Advanced Photonics   4 ( 1 )   2022.1   eISSN:2577-5421

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    Broadband Raman spectroscopy (detection bandwidth >1000 cm-1) is a valuable and widely used tool for understanding samples via label-free measurements of their molecular vibrations. Two important Raman spectral regions are the chemically specific "fingerprint"(200 to 1800 cm-1) and "low-frequency"or "terahertz"(THz) (<200 cm-1; <6 THz) regions, which mostly contain intramolecular and intermolecular vibrations, respectively. These two regions are highly complementary; broadband simultaneous measurement of both regions can provide a big picture comprising information about molecular structures and interactions. Although techniques for acquiring broadband Raman spectra covering both regions have been demonstrated, these methods tend to have spectral acquisition rates <10 spectra/s, prohibiting high-speed applications, such as Raman imaging or vibrational detection of transient phenomena. Here, we demonstrate a single-laser method for ultrafast (24,000 spectra/s) broadband Raman spectroscopy covering both THz and fingerprint regions. This is achieved by simultaneous detection of Sagnac-enhanced impulsive stimulated Raman scattering (SE-ISRS; THz-sensitive) and Fourier-transform coherent anti-Stokes Raman scattering (FT-CARS; fingerprint-sensitive). With dual-detection impulsive vibrational spectroscopy, the SE-ISRS signal shows a >500 × enhancement of <6.5 THz sensitivity compared with that of FT-CARS, and the FT-CARS signal shows a >10 × enhancement of fingerprint sensitivity above 1000 cm-1 compared with that of SE-ISRS.

    DOI: 10.1117/1.AP.4.1.016003

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  • Massive image-based single-cell profiling reveals high levels of circulating platelet aggregates in patients with COVID-19

    Masako Nishikawa, Hiroshi Kanno, Yuqi Zhou, Ting Hui Xiao, Takuma Suzuki, Yuma Ibayashi, Jeffrey Harmon, Shigekazu Takizawa, Kotaro Hiramatsu, Nao Nitta, Risako Kameyama, Walker Peterson, Jun Takiguchi, Mohammad Shifat-E-Rabbi, Yan Zhuang, Xuwang Yin, Abu Hasnat Mohammad Rubaiyat, Yunjie Deng, Hongqian Zhang, Shigeki Miyata, Gustavo K. Rohde, Wataru Iwasaki, Yutaka Yatomi, Keisuke Goda

    Nature Communications   12 ( 1 )   2021.12

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    A characteristic clinical feature of COVID-19 is the frequent incidence of microvascular thrombosis. In fact, COVID-19 autopsy reports have shown widespread thrombotic microangiopathy characterized by extensive diffuse microthrombi within peripheral capillaries and arterioles in lungs, hearts, and other organs, resulting in multiorgan failure. However, the underlying process of COVID-19-associated microvascular thrombosis remains elusive due to the lack of tools to statistically examine platelet aggregation (i.e., the initiation of microthrombus formation) in detail. Here we report the landscape of circulating platelet aggregates in COVID-19 obtained by massive single-cell image-based profiling and temporal monitoring of the blood of COVID-19 patients (n = 110). Surprisingly, our analysis of the big image data shows the anomalous presence of excessive platelet aggregates in nearly 90&#37; of all COVID-19 patients. Furthermore, results indicate strong links between the concentration of platelet aggregates and the severity, mortality, respiratory condition, and vascular endothelial dysfunction level of COVID-19 patients.

    DOI: 10.1038/s41467-021-27378-2

  • Highly sensitive Fourier-transform coherent anti-Stokes Raman scattering spectroscopy via genetic algorithm pulse shaping. Reviewed International journal

    Matthew Lindley, Julia Gala de Pablo, Ryo Kinegawa, Kotaro Hiramatsu, Keisuke Goda

    Optics letters   46 ( 17 )   4320 - 4323   2021.9

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    We report highly sensitive Fourier-transform coherent anti-Stokes Raman scattering spectroscopy enabled by genetic algorithm (GA) pulse shaping for adaptive dispersion compensation. We show that the non-resonant four-wave mixing signal from water can be used as a fitness indicator for successful GA training. This method allows GA adaptation to sample measurement conditions and offers significantly improved performance compared to training using second-harmonic generation from a nonlinear crystal in place of the sample. Results include a 3× improvement to peak signal-to-noise ratio for 2-propanol measurement, as well as a 10× improvement to peak intensities from the high-throughput measurement of polystyrene microbeads under flow.

    DOI: 10.1364/OL.434054

  • Highly Sensitive Low-Frequency Time-Domain Raman Spectroscopy via Fluorescence Encoding. Reviewed International journal

    Phillip C McCann, Kotaro Hiramatsu, Keisuke Goda

    The journal of physical chemistry letters   12 ( 32 )   7859 - 7865   2021.8

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    Fluorescence-encoded vibrational spectroscopy has become increasingly more popular by virtue of its high chemical specificity and sensitivity. However, current fluorescence-encoded vibrational spectroscopy methods lack sensitivity in the low-frequency region, which if addressed could further enhance their capabilities. Here, we present a method for highly sensitive low-frequency fluorescence-encoded vibrational spectroscopy, termed fluorescence-encoded time-domain coherent Raman spectroscopy (FLETCHERS). By first exciting molecules into vibrationally excited states and then promoting the vibrating molecules to electronic states at varying times, the molecular vibrations can be encoded onto the emitted time-domain fluorescence intensity. We demonstrate the sensitive low-frequency detection capability of FLETCHERS by measuring vibrational spectra in the lower fingerprint region of rhodamine 800 solutions as dilute as 250 nM, which is ∼1000 times more sensitive than conventional vibrational spectroscopy. These results, along with further improvement of the method, open up the prospect of performing single-molecule vibrational spectroscopy in the low-frequency region.

    DOI: 10.1021/acs.jpclett.1c01741

  • Morphological Indicator for Directed Evolution of Euglena gracilis with a High Heavy Metal Removal Efficiency. Reviewed International journal

    Muzhen Xu, Jeffrey Harmon, Dan Yuan, Sheng Yan, Cheng Lei, Kotaro Hiramatsu, Yuqi Zhou, Mun Hong Loo, Tomohisa Hasunuma, Akihiro Isozaki, Keisuke Goda

    Environmental science & technology   55 ( 12 )   7880 - 7889   2021.6

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    In the past few decades, microalgae-based bioremediation methods for treating heavy metal (HM)-polluted wastewater have attracted much attention by virtue of their environment friendliness, cost efficiency, and sustainability. However, their HM removal efficiency is far from practical use. Directed evolution is expected to be effective for developing microalgae with a much higher HM removal efficiency, but there is no non-invasive or label-free indicator to identify them. Here, we present an intelligent cellular morphological indicator for identifying the HM removal efficiency of Euglena gracilis in a non-invasive and label-free manner. Specifically, we show a strong monotonic correlation (Spearman's ρ = -0.82, P = 2.1 × 10-5) between a morphological meta-feature recognized via our machine learning algorithms and the Cu2+ removal efficiency of 19 E. gracilis clones. Our findings firmly suggest that the morphology of E. gracilis cells can serve as an effective HM removal efficiency indicator and hence have great potential, when combined with a high-throughput image-activated cell sorter, for directed-evolution-based development of E. gracilis with an extremely high HM removal efficiency for practical wastewater treatment worldwide.

    DOI: 10.1021/acs.est.0c05278

  • High-Throughput Raman Flow Cytometry and Beyond. Reviewed International journal

    Julia Gala de Pablo, Matthew Lindley, Kotaro Hiramatsu, Keisuke Goda

    Accounts of chemical research   54 ( 9 )   2132 - 2143   2021.5

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    Flow cytometry is a powerful tool with applications in diverse fields such as microbiology, immunology, virology, cancer biology, stem cell biology, and metabolic engineering. It rapidly counts and characterizes large heterogeneous populations of cells in suspension (e.g., blood cells, stem cells, cancer cells, and microorganisms) and dissociated solid tissues (e.g., lymph nodes, spleen, and solid tumors) with typical throughputs of 1,000-100,000 events per second (eps). By measuring cell size, cell granularity, and the expression of cell surface and intracellular molecules, it provides systematic insights into biological processes. Flow cytometers may also include cell sorting capabilities to enable subsequent additional analysis of the sorted sample (e.g., electron microscopy and DNA/RNA sequencing), cloning, and directed evolution. Unfortunately, traditional flow cytometry has several critical limitations as it mainly relies on fluorescent labeling for cellular phenotyping, which is an indirect measure of intracellular molecules and surface antigens. Furthermore, it often requires time-consuming preparation protocols and is incompatible with cell therapy. To overcome these difficulties, a different type of flow cytometry based on direct measurements of intracellular molecules by Raman spectroscopy, or "Raman flow cytometry" for short, has emerged. Raman flow cytometry obtains a chemical fingerprint of the cell in a nondestructive manner, allowing for single-cell metabolic phenotyping. However, its slow signal acquisition due to the weak light-molecule interaction of spontaneous Raman scattering prevents the throughput necessary to interrogate large cell populations in reasonable time frames, resulting in throughputs of about 1 eps. The remedy to this throughput limit lies in coherent Raman scattering methods such as stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS), which offer a significantly enhanced light-sample interaction and hence enable high-throughput Raman flow cytometry, Raman imaging flow cytometry, and even Raman image-activated cell sorting (RIACS). In this Account, we outline recent advances, technical challenges, and emerging opportunities of coherent Raman flow cytometry. First, we review the principles of various types of SRS and CARS and introduce several techniques of coherent Raman flow cytometry such as CARS, multiplex CARS, Fourier-transform CARS, SRS, SRS imaging flow cytometry, and RIACS. Next, we discuss a unique set of applications enabled by coherent Raman flow cytometry, from microbiology and lipid biology to cancer detection and cell therapy. Finally, we describe future opportunities and challenges of coherent Raman flow cytometry including increasing sensitivity and throughput, integration with droplet microfluidics, utilizing machine learning techniques, or achieving in vivo flow cytometry. This Account summarizes the growing field of high-throughput Raman flow cytometry and the bright future it can bring.

    DOI: 10.1021/acs.accounts.1c00001

  • All-dielectric chiral-field-enhanced Raman optical activity. Reviewed International journal

    Ting-Hui Xiao, Zhenzhou Cheng, Zhenyi Luo, Akihiro Isozaki, Kotaro Hiramatsu, Tamitake Itoh, Masahiro Nomura, Satoshi Iwamoto, Keisuke Goda

    Nature communications   12 ( 1 )   3062 - 3062   2021.5

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    Raman optical activity (ROA) is effective for studying the conformational structure and behavior of chiral molecules in aqueous solutions and is advantageous over X-ray crystallography and nuclear magnetic resonance spectroscopy in sample preparation and cost performance. However, ROA signals are inherently minuscule; 3-5 orders of magnitude weaker than spontaneous Raman scattering due to the weak chiral light-matter interaction. Localized surface plasmon resonance on metallic nanoparticles has been employed to enhance ROA signals, but suffers from detrimental spectral artifacts due to its photothermal heat generation and inability to efficiently transfer and enhance optical chirality from the far field to the near field. Here we demonstrate all-dielectric chiral-field-enhanced ROA by devising a silicon nanodisk array and exploiting its dark mode to overcome these limitations. Specifically, we use it with pairs of chemical and biological enantiomers to show >100x enhanced chiral light-molecule interaction with negligible artifacts for ROA measurements.

    DOI: 10.1038/s41467-021-23364-w

  • Dual-Comb Coherent Raman Spectroscopy with near 100&#37; Duty Cycle Reviewed International journal

    Risako Kameyama, Shigekazu Takizawa, Kotaro Hiramatsu, Keisuke Goda

    ACS PHOTONICS   8 ( 4 )   975 - 981   2021.4

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    Dual-comb coherent Raman spectroscopy is a powerful tool for rapidly probing vibrational signatures of molecules in the fingerprint region. However, >99&#37; of its incident laser energy is unused and wasted since the duty cycle of its spectral acquisition is only less than 1&#37; due to the mismatch between the interval of the laser pulses (>1 ns) and the coherence lifetime of molecular vibrations (similar to 3 ps). Here we demonstrate similar to 100&#37; duty-cycle dual-comb coherent Raman spectroscopy with a "quasi"-dual-comb source. This is made possible by rapidly modulating the cavity length of one of the frequency combs and accurately measuring the group delay between pump and probe pulses by two-color interferometry for calibrating the phase of each Raman active mode in the sample. Specifically, we use the method to show a record high spectral acquisition rate of 100000 spectra/s with even higher sensitivity than conventional slower dual-comb coherent Raman spectroscopy.

    DOI: 10.1021/acsphotonics.0c01656

  • Porous carbon nanowire array for surface-enhanced Raman spectroscopy. Reviewed International journal

    Nan Chen, Ting-Hui Xiao, Zhenyi Luo, Yasutaka Kitahama, Kotaro Hiramatsu, Naoki Kishimoto, Tamitake Itoh, Zhenzhou Cheng, Keisuke Goda

    Nature communications   11 ( 1 )   4772 - 4772   2020.9

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    Surface-enhanced Raman spectroscopy (SERS) is a powerful tool for vibrational spectroscopy as it provides several orders of magnitude higher sensitivity than inherently weak spontaneous Raman scattering by exciting localized surface plasmon resonance (LSPR) on metal substrates. However, SERS can be unreliable for biomedical use since it sacrifices reproducibility, uniformity, biocompatibility, and durability due to its strong dependence on "hot spots", large photothermal heat generation, and easy oxidization. Here, we demonstrate the design, fabrication, and use of a metal-free (i.e., LSPR-free), topologically tailored nanostructure composed of porous carbon nanowires in an array as a SERS substrate to overcome all these problems. Specifically, it offers not only high signal enhancement (~106) due to its strong broadband charge-transfer resonance, but also extraordinarily high reproducibility due to the absence of hot spots, high durability due to no oxidization, and high compatibility to biomolecules due to its fluorescence quenching capability.

    DOI: 10.1038/s41467-020-18590-7

  • Intelligent image-activated cell sorting 2.0 Reviewed International journal

    Akihiro Isozaki, Hideharu Mikami, Hiroshi Tezuka, Hiroki Matsumura, Kangrui Huang, Marino Akamine, Kotaro Hiramatsu, Takanori Iino, Takuro Ito, Hiroshi Karakawa, Yusuke Kasai, Yan Li, Yuta Nakagawa, Shinsuke Ohnuki, Tadataka Ota, Yong Qian, Shinya Sakuma, Takeichiro Sekiya, Yoshitaka Shirasaki, Nobutake Suzuki, Ehsen Tayyabi, Tsubasa Wakamiya, Muzhen Xu, Mai Yamagishi, Haochen Yan, Qiang Yu, Sheng Yan, Dan Yuan, Wei Zhang, Yaqi Zhao, Fumihito Arai, Robert E. Campbell, Christophe Danelon, Dino Di Carlo, Kei Hiraki, Yu Hoshino, Yoichiroh Hosokawa, Mary Inaba, Atsuhiro Nakagawa, Yoshikazu Ohya, Minoru Oikawa, Sotaro Uemura, Yasuyuki Ozeki, Takeaki Sugimura, Nao Nitta, Keisuke Goda

    LAB ON A CHIP   20 ( 13 )   2263 - 2273   2020.7

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    The advent of intelligent image-activated cell sorting (iIACS) has enabled high-throughput intelligent image-based sorting of single live cells from heterogeneous populations. iIACS is an on-chip microfluidic technology that builds on a seamless integration of a high-throughput fluorescence microscope, cell focuser, cell sorter, and deep neural network on a hybrid software-hardware data management architecture, thereby providing the combined merits of optical microscopy, fluorescence-activated cell sorting (FACS), and deep learning. Here we report an iIACS machine that far surpasses the state-of-the-art iIACS machine in system performance in order to expand the range of applications and discoveries enabled by the technology. Specifically, it provides a high throughput of similar to 2000 events per second and a high sensitivity of similar to 50 molecules of equivalent soluble fluorophores (MESFs), both of which are 20 times superior to those achieved in previous reports. This is made possible by employing (i) an image-sensor-based optomechanical flow imaging method known as virtual-freezing fluorescence imaging and (ii) a real-time intelligent image processor on an 8-PC server equipped with 8 multi-core CPUs and GPUs for intelligent decision-making, in order to significantly boost the imaging performance and computational power of the iIACS machine. We characterize the iIACS machine with fluorescent particles and various cell types and show that the performance of the iIACS machine is close to its achievable design specification. Equipped with the improved capabilities, this new generation of the iIACS technology holds promise for diverse applications in immunology, microbiology, stem cell biology, cancer biology, pathology, and synthetic biology.

    DOI: 10.1039/d0lc00080a

  • Raman image-activated cell sorting Reviewed International journal

    Nao Nitta, Takanori Iino, Akihiro Isozaki, Mai Yamagishi, Yasutaka Kitahama, Shinya Sakuma, Yuta Suzuki, Hiroshi Tezuka, Minoru Oikawa, Fumihito Arai, Takuya Asai, Dinghuan Deng, Hideya Fukuzawa, Misa Hase, Tomohisa Hasunuma, Takeshi Hayakawa, Kei Hiraki, Kotaro Hiramatsu, Yu Hoshino, Mary Inaba, Yuki Inoue, Takuro Ito, Masataka Kajikawa, Hiroshi Karakawa, Yusuke Kasai, Yuichi Kato, Hirofumi Kobayashi, Cheng Lei, Satoshi Matsusaka, Hideharu Mikami, Atsuhiro Nakagawa, Keiji Numata, Tadataka Ota, Takeichiro Sekiya, Kiyotaka Shiba, Yoshitaka Shirasaki, Nobutake Suzuki, Shunji Tanaka, Shunnosuke Ueno, Hiroshi Watarai, Takashi Yamano, Masayuki Yazawa, Yusuke Yonamine, Dino Di Carlo, Yoichiroh Hosokawa, Sotaro Uemura, Takeaki Sugimura, Yasuyuki Ozeki, Keisuke Goda

    NATURE COMMUNICATIONS   11 ( 1 )   3452 - 3452   2020.7

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    The advent of image-activated cell sorting and imaging-based cell picking has advanced our knowledge and exploitation of biological systems in the last decade. Unfortunately, they generally rely on fluorescent labeling for cellular phenotyping, an indirect measure of the molecular landscape in the cell, which has critical limitations. Here we demonstrate Raman image-activated cell sorting by directly probing chemically specific intracellular molecular vibrations via ultrafast multicolor stimulated Raman scattering (SRS) microscopy for cellular phenotyping. Specifically, the technology enables real-time SRS-image-based sorting of single live cells with a throughput of up to similar to 100 events per second without the need for fluorescent labeling. To show the broad utility of the technology, we show its applicability to diverse cell types and sizes. The technology is highly versatile and holds promise for numerous applications that are previously difficult or undesirable with fluorescence-based technologies.

    DOI: 10.1038/s41467-020-17285-3

  • Spatiotemporal monitoring of intracellular metabolic dynamics by resonance Raman microscopy with isotope labeling Reviewed International journal

    Yusuke Yonamine, Kotaro Hiramatsu, Takuro Ideguchi, Takuro Ito, Tomomi Fujiwara, Yoshiko Miura, Keisuke Goda, Yu Hoshino

    RSC ADVANCES   10 ( 28 )   16679 - 16686   2020.4

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    Cellular metabolites are valuable in a diverse range of applications. For example, the unicellular green algaHaematococcus lacustrisproduces as a secondary metabolite the carotenoid pigment astaxanthin (AXT), which is widely used in nutraceutical, cosmetic, and food industries due to its strong antioxidant activity. In order to enhance the productivity ofH. lacustris, spatial and temporal understanding of its metabolic dynamics is essential. Here we show spatiotemporal monitoring of AXT production inH. lacustriscells by resonance Raman microscopy combined with stable isotope labeling. Specifically, we incorporated carbon dioxide ((CO2)-C-13) labeled with a stable isotope (C-13) intoH. lacustriscells through carbon fixation and traced its conversion to(13)C-AXT using our resonance Raman microscope. We incubatedH. lacustriscells under various conditions by switching, pulsing, and replacing(13)CO(2)and(12)CO(2). By measurement of these cells we determined the fixation time of(13)C-carbon, visualized the intracellular localization of(13)C- and(12)C-AXTs, and revealed the dynamic consumption-production equilibrium of the accumulated AXT. This work is a valuable step in the development of effective screening criteria for high AXT-producingH. lacustriscells.

    DOI: 10.1039/d0ra02803g

  • Large-scale label-free single-cell analysis of paramylon in Euglena gracilis by high-throughput broadband Raman flow cytometry. Reviewed International journal

    Kotaro Hiramatsu, Koji Yamada, Matthew Lindley, Kengo Suzuki, Keisuke Goda

    11 ( 4 )   1752 - 1759   2020.4

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    Large-scale label-free single-cell analysis of paramylon in Euglena gracilis by high-throughput broadband Raman flow cytometry.
    Microalga-based biomaterial production has attracted attention as a new source of drugs, foods, and biofuels. For enhancing the production efficiency, it is essential to understand its differences between heterogeneous microalgal subpopulations. However, existing techniques are not adequate to address the need due to the lack of single-cell resolution or the inability to perform large-scale analysis and detect small molecules. Here we demonstrated large-scale single-cell analysis of Euglena gracilis (a unicellular microalgal species that produces paramylon as a potential drug for HIV and colon cancer) with our recently developed high-throughput broadband Raman flow cytometer at a throughput of >1,000 cells/s. Specifically, we characterized the intracellular content of paramylon from single-cell Raman spectra of 10,000 E. gracilis cells cultured under five different conditions and found that paramylon contents in E. gracilis cells cultured in an identical condition is given by a log-normal distribution, which is a good model for describing the number of chemicals in a reaction network. The capability of characterizing distribution functions in a label-free manner is an important basis for isolating specific cell populations for synthetic biology via directed evolution based on the intracellular content of metabolites.

    DOI: 10.1364/BOE.382957

  • Compressed time-domain coherent Raman spectroscopy with real-time random sampling Reviewed International journal

    Shigekazu Takizawa, Kotaro Hiramatsu, Keisuke Goda

    VIBRATIONAL SPECTROSCOPY   107   2020.3

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    We experimentally demonstrate compressed time-domain coherent Raman spectroscopy with real-time random sampling. Specifically, we employ Fourier-transform coherent anti-Stokes Raman scattering as a platform for the spectroscopy and demonstrate reconstruction of Raman spectra from sparsely sampled time-domain inter-ferograms using the iterative shrinkage-thresholding algorithm. We show that Raman spectra reconstructed at compression ratios down to 0.3 retain the quality of a fully sampled Raman spectrum. We also verify this performance with multiple molecules to demonstrate its versatility. Advantages of our method include data volume reduction, signal acquisition time reduction, and alleviated requirements for measurement instruments. (C) 2019 Optical Society of America.

    DOI: 10.1016/j.vibspec.2020.103042

  • Ultrafast Simultaneous Raman-Fluorescence Spectroscopy Reviewed International journal

    Matthew Lindley, Kotaro Hiramatsu, Hayate Nomoto, Fukashi Shibata, Tsuyoshi Takeshita, Shigeyuki Kawano, Keisuke Goda

    ANALYTICAL CHEMISTRY   91 ( 24 )   15563 - 15569   2019.12

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    Raman and fluorescence spectroscopies offer complementary approaches in bioanalytical chemistry, particularly in microbiological assays. The former method is used to detect lipids, metabolites, and nonspecific proteins and nucleic acids in a label-free manner, while the latter is used to investigate targeted proteins, nucleic acids, and their interactions via labeling or transfection. Despite their complementarity, these regimes are seldom used in conjunction due to fluorescent signals overwhelming inherently weak Raman signals by more than several orders of magnitude. Here we report a multimodal spectrometer that simultaneously performs Raman and fluorescence spectroscopies at high speed. It is made possible by Fourier-transform-coherent anti-Stokes Raman scattering (FT-CARS) and Fourier-transform-two-photon excitation (FT-TPE) measurements powered by a femtosecond pulse laser coupled to a homemade rapid-scan Michelson interferometer, operating at 24 000 spectra per second. As a proof-of-principle demonstration, we validate the ultrafast fluoRaman spectrometer by measuring coumarin dyes in organic solvents. To show its potential for applications that require rapid fluoRaman spectroscopy, we also demonstrate fluoRaman flow cytometry of Haematococcus pluvialis cells under varying culture conditions with a high throughput of similar to 10 events per second to perform large-scale single-cell analysis of their metabolic stress response.

    DOI: 10.1021/acs.analchem.9b03563

  • Sagnac-enhanced impulsive stimulated Raman scattering for highly sensitive low-frequency Raman spectroscopy Reviewed International journal

    Walker Peterson, Kotaro Hiramatsu, Keisuke Goda

    OPTICS LETTERS   44 ( 21 )   5282 - 5285   2019.11

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    The "fingerprint" (500-1800 cm(-1)) and "high-frequency" (2400-4000 cm(-1)) regions in Raman spectroscopy are commonly used for label-free chemical analysis, while the "low-frequency" region (<200 cm(-1)) is often overlooked, despite containing rich information. This is largely due to the challenge of measuring weak Raman signals that are obscured by strong Rayleigh scattering. Here we propose and experimentally demonstrate Sagnac-enhanced impulsive stimulated Raman scattering (SE-ISRS), a filter-free method for time-domain Raman spectroscopy that overcomes the challenge and provides low-frequency Raman spectra at all probe frequencies. Using SE-ISRS for simultaneous low-frequency and fingerprint region measurements, we demonstrate a >5x enhancement of the signal-to-noise ratio compared to conventional ISRS spectroscopy. (C) 2019 Optical Society of America

    DOI: 10.1364/OL.44.005282

  • A practical guide to intelligent image-activated cell sorting (vol 14, pg 2370, 2019) Reviewed International journal

    Akihiro Isozaki, Hideharu Mikami, Kotaro Hiramatsu, Shinya Sakuma, Yusuke Kasai, Takanori Iino, Takashi Yamano, Atsushi Yasumoto, Yusuke Oguchi, Nobutake Suzuki, Yoshitaka Shirasaki, Taichiro Endo, Takuro Ito, Kei Hiraki, Makoto Yamada, Satoshi Matsusaka, Takeshi Hayakawa, Hideya Fukuzawa, Yutaka Yatomi, Fumihito Arai, Dino Di Carlo, Atsuhiro Nakagawa, Yu Hoshino, Yoichiroh Hosokawa, Sotaro Uemura, Takeaki Sugimura, Yasuyuki Ozeki, Nao Nitta, Keisuke Goda

    NATURE PROTOCOLS   14 ( 11 )   3273 - 3273   2019.11

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    An amendment to this paper has been published and can be accessed via a link at the top of the paper.

    DOI: 10.1038/s41596-019-0252-5

  • A practical guide to intelligent image-activated cell sorting Reviewed International journal

    Akihiro Isozaki, Hideharu Mikami, Kotaro Hiramatsu, Shinya Sakuma, Yusuke Kasai, Takanori Iino, Takashi Yamano, Atsushi Yasumoto, Yusuke Oguchi, Nobutake Suzuki, Yoshitaka Shirasaki, Taichiro Endo, Takuro Ito, Kei Hiraki, Makoto Yamada, Satoshi Matsusaka, Takeshi Hayakawa, Hideya Fukuzawa, Yutaka Yatomi, Fumihito Arai, Dino Di Carlo, Atsuhiro Nakagawa, Yu Hoshino, Yoichiroh Hosokawa, Sotaro Uemura, Takeaki Sugimura, Yasuyuki Ozeki, Nao Nitta, Keisuke Goda

    NATURE PROTOCOLS   14 ( 8 )   2370 - 2415   2019.8

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    Intelligent image-activated cell sorting (iIACS) is a machine-intelligence technology that performs real-time intelligent image-based sorting of single cells with high throughput. iIACS extends beyond the capabilities of fluorescence-activated cell sorting (FACS) from fluorescence intensity profiles of cells to multidimensional images, thereby enabling high-content sorting of cells or cell clusters with unique spatial chemical and morphological traits. Therefore, iIACS serves as an integral part of holistic single-cell analysis by enabling direct links between population-level analysis (flow cytometry), cell-level analysis (microscopy), and gene-level analysis (sequencing). Specifically, iIACS is based on a seamless integration of high-throughput cell microscopy (e.g., multicolor fluorescence imaging, bright-field imaging), cell focusing, cell sorting, and deep learning on a hybrid software-hardware data management infrastructure, enabling real-time automated operation for data acquisition, data processing, intelligent decision making, and actuation. Here, we provide a practical guide to iIACS that describes how to design, build, characterize, and use an iIACS machine. The guide includes the consideration of several important design parameters, such as throughput, sensitivity, dynamic range, image quality, sort purity, and sort yield; the development and integration of optical, microfluidic, electrical, computational, and mechanical components; and the characterization and practical usage of the integrated system. Assuming that all components are readily available, a team of several researchers experienced in optics, electronics, digital signal processing, microfluidics, mechatronics, and flow cytometry can complete this protocol in similar to 3 months.

    DOI: 10.1038/s41596-019-0183-1

  • High-speed broadband Fourier-transform coherent anti-stokes Raman scattering spectral microscopy Reviewed International journal

    Ryo Kinegawa, Kotaro Hiramatsu, Kazuki Hashimoto, Venkata Ramaiah Badarla, Takuro Ideguchi, Keisuke Goda

    JOURNAL OF RAMAN SPECTROSCOPY   50 ( 8 )   1141 - 1146   2019.8

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    We demonstrate broadband Fourier-transform coherent anti-Stokes Raman scattering (FT-CARS) spectral microscopy with a pixel dwell time of 42 mu s, which is ~50 times shorter than the shortest-to-date pixel dwell time for CARS spectral microscopy. Our broadband FT-CARS spectral microscope is composed of an FT-CARS spectrometer, a rapid galvanometric scanner, and a high-speed image acquisition circuit, enabling a frame rate of 2.4 fps with a pixel resolution of 100 x 100 pixels, a bandwidth of 600-1,200 cm(-1), a spatial resolution of 0.95 mu m, and a spectral resolution of 37 cm(-1). As a proof-of-principle demonstration, we used the high-speed FT-CARS spectral microscope to perform CARS imaging of polymer beads and Haematococcus lacustris cells. Our high-speed broadband CARS spectral microscope holds promise for studying rapid cellular dynamics, such as signaling, cell-to-cell communication, and molecular transport in a label-free manner.

    DOI: 10.1002/jrs.5630

  • High-throughput label-free molecular fingerprinting flow cytometry Reviewed International journal

    Kotaro Hiramatsu, Takuro Ideguchi, Yusuke Yonamine, SangWook Lee, Yizhi Luo, Kazuki Hashimoto, Takuro Ito, Misa Hase, Jee-Woong Park, Yusuke Kasai, Shinya Sakuma, Takeshi Hayakawa, Fumihito Arai, Yu Hoshino, Keisuke Goda

    SCIENCE ADVANCES   5 ( 1 )   eaau0241   2019.1

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    Flow cytometry is an indispensable tool in biology for counting and analyzing single cells in large heterogeneous populations. However, it predominantly relies on fluorescent labeling to differentiate cells and, hence, comes with several fundamental drawbacks. Here, we present a high-throughput Raman flow cytometer on a microfluidic chip that chemically probes single live cells in a label-free manner. It is based on a rapid-scan Fourier-transform coherent anti-Stokes Raman scattering spectrometer as an optical interrogator, enabling us to obtain the broadband molecular vibrational spectrum of every single cell in the fingerprint region (400 to 1600 cm(-1)) with a record--high throughput of similar to 2000 events/s. As a practical application of the method not feasible with conventional flow cytometry, we demonstrate high-throughput label-free single-cell analysis of the astaxanthin productivity and photosynthetic dynamics of Haematococcus lacustris.

    DOI: 10.1126/sciadv.aau0241

  • 超高速キラル分光法 Invited Reviewed

    平松光太郎

    分光研究   67 ( 4 )   144 - 153   2018.10

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    Ultrafast chiroptical spectroscopy

  • Intelligent Image-Activated Cell Sorting Reviewed International journal

    Nao Nitta, Takeaki Sugimura, Akihiro Isozaki, Hideharu Mikami, Kei Hiraki, Shinya Sakuma, Takanori Iino, Fumihito Arai, Taichiro Endo, Yasuhiro Fujiwaki, Hideya Fukuzawa, Misa Hase, Takeshi Hayakawa, Kotaro Hiramatsu, Yu Hoshino, Mary Inaba, Takuro Ito, Hiroshi Karakawa, Yusuke Kasai, Kenichi Koizumi, SangWook Lee, Cheng Lei, Ming Li, Takanori Maeno, Satoshi Matsusaka, Daichi Murakami, Atsuhiro Nakagawa, Yusuke Oguchi, Minoru Oikawa, Tadataka Ota, Kiyotaka Shiba, Hirofumi Shintaku, Yoshitaka Shirasaki, Kanako Suga, Yuta Suzuki, Nobutake Suzuki, Yo Tanaka, Hiroshi Tezuka, Chihana Toyokawa, Yaxiaer Yalikun, Makoto Yamada, Mai Yamagishi, Takashi Yamano, Atsushi Yasumoto, Yutaka Yatomi, Masayuki Yazawa, Dino Di Carlo, Yoichiroh Hosokawa, Sotaro Uemura, Yasuyuki Ozeki, Keisuke Goda

    CELL   175 ( 1 )   266 - +   2018.9

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    A fundamental challenge of biology is to understand the vast heterogeneity of cells, particularly how cellular composition, structure, and morphology are linked to cellular physiology. Unfortunately, conventional technologies are limited in uncovering these relations. We present a machine-intelligence technology based on a radically different architecture that realizes real-time image-based intelligent cell sorting at an unprecedented rate. This technology, which we refer to as intelligent image-activated cell sorting, integrates high-throughput cell microscopy, focusing, and sorting on a hybrid software-hardware data-management infrastructure, enabling real-time automated operation for data acquisition, data processing, decision-making, and actuation. We use it to demonstrate real-time sorting of microalgal and blood cells based on intracellular protein localization and cell-cell interaction from large heterogeneous populations for studying photosynthesis and atherothrombosis, respectively. The technology is highly versatile and expected to enable machine-based scientific discovery in biological, pharmaceutical, and medical sciences.

    DOI: 10.1016/j.cell.2018.08.028

  • Microfluidic single-particle chemical analyzer with dual-comb coherent Raman spectroscopy Reviewed International journal

    Takuro Ideguchi, Tasuku Nakamura, Shigekazu Takizawa, Miu Tamamitsu, Sangwook Lee, Kotaro Hiramatsu, Venkata Ramaiah-Badarla, Jee-woong Park, Yusuke Kasai, Takeshi Hayakawa, Shinya Sakuma, Fumihito Arai, Keisuke Goda

    OPTICS LETTERS   43 ( 16 )   4057 - 4060   2018.8

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    Label-free particle analysis is a powerful tool in chemical, pharmaceutical, and cosmetic industries as well as in basic sciences, but its throughput is significantly lower than that of fluorescence-based counterparts. Here we present a label-free single-particle analyzer based on broadband dual-comb coherent Raman scattering spectroscopy operating at a spectroscopic scan rate of 10 kHz. As a proof-of-concept demonstration, we perform broadband coherent anti-Stokes Raman scattering measurements of polystyrene microparticles flowing on an acoustofluidic chip at a high throughput of >1000 particles per second. This high-throughput label-free particle analyzer has the potential for high-precision statistical analysis of a large number of microparticles including biological cells. (C) 2018 Optical Society of America

    DOI: 10.1364/OL.43.004057

  • Development of a Hyper-Raman Microspectroscopic System Using a Wavelength-tunable Laser Source Reviewed

    Yoshiharu Yamada, Naoki Fukutake, Kotaro Hiramatsu, Hideaki Kano

    CHEMISTRY LETTERS   47 ( 5 )   660 - 663   2018.5

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    In this study, we developed a hyper-Raman (HR) microscopic system using an optical parametric oscillator (OPO) pumped by a picosecond mode-locked Nd:YVO4 laser source. Owing to the wavelength tunability of OPO, the two-photon electronic resonance effect can be investigated in a broad spectral range of 345-495 nm. As a proof-of-principle experiment, we performed HR imaging of TiO2 single crystals. In addition to HR, second harmonic generation (SHG) and two-photon excitation fluorescence (TPEF) were observed simultaneously depending on the excitation wavelength.

    DOI: 10.1246/cl.180077

  • Rapid-scan Fourier-transform coherent anti-Stokes Raman scattering spectroscopy with heterodyne detection Reviewed International journal

    Kotaro Hiramatsu, Yizhi Luo, Takuro Ideguchi, Keisuke Goda

    OPTICS LETTERS   42 ( 21 )   4335 - 4338   2017.11

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    High-speed Raman spectroscopy has become increasingly important for analyzing chemical dynamics in real time. To address the need, rapid-scan Fourier-transform coherent anti-Stokes Raman scattering (FT-CARS) spectroscopy has been developed to realize broadband CARS measurements at a scan rate of more than 20,000 scans/s. However, the detection sensitivity of FT-CARS spectroscopy is inherently low due to the limited number of photons detected during each scan. In this Letter, we show our experimental demonstration of enhanced sensitivity in rapid-scan FT-CARS spectroscopy by heterodyne detection. Specifically, we implemented heterodyne detection by superposing the CARS electric field with an external local oscillator (LO) for their interference. The CARS signal was amplified by simply increasing the power of the LO without the need for increasing the incident power onto the sample. Consequently, we achieved enhancement in signal intensity and the signal-to-noise ratio by factors of 39 and 5, respectively, compared to FT-CARS spectroscopy with homodyne detection. The sensitivity-improved rapid-scan FT-CARS spectroscopy is expected to enable the sensitive real-time observation of chemical dynamics in a broad range of settings, such as combustion engines and live biological cells. (C) 2017 Optical Society of America

    DOI: 10.1364/OL.42.004335

  • Raman optical activity spectroscopy by visible-excited coherent anti-Stokes Raman scattering Reviewed International journal

    Kotaro Hiramatsu, Philippe Leproux, Vincent Couderc, Takashi Nagata, Hideaki Kano

    OPTICS LETTERS   40 ( 17 )   4170 - 4173   2015.9

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    We developed a Raman optical activity (ROA) spectroscopic system with visible-excited coherent anti-Stokes Raman scattering (CARS). A supercontinuum within the visible region was generated with a photonic crystal fiber pumped with both 532 and 1064 nm excitation, generating a multiplexed CARS-ROA spectrum covering the whole fingerprint region. In visible excitation, the CARS-ROA spectrum of (-)-beta-pinene shows a higher contrast ratio of the chirality-induced signal to the achiral background than that of the previously reported near-infrared CARS-ROA spectrum. (C) 2015 Optical Society of America

    DOI: 10.1364/OL.40.004170

  • Communication: Broadband and ultrasensitive femtosecond time-resolved circular dichroism spectroscopy Reviewed International journal

    Kotaro Hiramatsu, Takashi Nagata

    JOURNAL OF CHEMICAL PHYSICS   143 ( 12 )   121102 - 121102   2015.9

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    We report the development of broadband and sensitive time-resolved circular dichroism (TRCD) spectroscopy by exploiting optical heterodyne detection. Using this method, transient CD signals of submillidegree level can be detected over the spectral range of 415-730 nm. We also demonstrate that the broadband measurement with the aid of singular value decomposition enables the discrimination of genuine TRCD signals from artificial optical-anisotropy, such as linear birefringence and linear dichroism, induced by photoexcitation. (C) 2015 AIP Publishing LLC.

    DOI: 10.1063/1.4932229

  • Raman optical activity by coherent anti-Stokes Raman scattering spectral interferometry Reviewed International journal

    Kotaro Hiramatsu, Hideaki Kano, Takashi Nagata

    OPTICS EXPRESS   21 ( 11 )   13515 - 13521   2013.6

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    We demonstrate a method to measure Raman optical activity (ROA) by using coherent anti-Stokes Raman scattering (CARS) spectral interferometry. An extremely weak chirality-induced CARS field is amplified through the interference with a strong CARS field generated from an external reference and is extracted by the Fourier transformation. In this interferometric coherent Raman optical activity (iCROA), both the sign and the magnitude of optical active non-resonant background susceptibility can be directly determined. Measurement of a CARS-ROA spectrum with less artifact is obtained because a broad offset artifact due to optical rotatory dispersion is clearly distinguished in iCROA. (C) 2013 Optical Society of America

    DOI: 10.1364/OE.21.013515

  • Observation of Raman Optical Activity by Heterodyne-Detected Polarization-Resolved Coherent Anti-Stokes Raman Scattering Reviewed International journal

    Kotaro Hiramatsu, Masanari Okuno, Hideaki Kano, Philippe Leproux, Vincent Couderc, Hiro-o Hamaguchi

    PHYSICAL REVIEW LETTERS   109 ( 8 )   083901 - 083901   2012.8

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    We report the first observation of Raman optical activity (ROA) by coherent anti-Stokes Raman scattering. Thanks to the more freedom of polarization configurations in coherent anti-Stokes Raman scattering than in spontaneous Raman spectroscopy, the contrast ratio of the chiral signal to the achiral background has been improved markedly. For (-)-beta-pinene, it is 2 orders of magnitude better than that in the reported spontaneous ROA measurement. This is also the first measurement of ROA signal using a pulsed laser source.

    DOI: 10.1103/PhysRevLett.109.083901

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Presentations

  • Time-domain Raman spectroscopy for large-scale cell screening Invited

    Kotaro Hiramatsu

    The 12th International Conference on Advanced Vibrational Spectroscopy  2023.8 

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    Time-domain Raman spectroscopy for large-scale cell screening

  • Time-domain Raman spectroscopy for large-scale cell screening Invited

    Kotaro Hiramatsu

    The 12th International Conference on Advanced Vibrational Spectroscopy  2023.8 

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    Event date: 2023.8 - 2023.9

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  • Raman-fingerprint-activated cell sorting Invited

    Kotaro Hiramatsu

    SciX2023  2023.8 

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    Raman-fingerprint-activated cell sorting

  • Raman-fingerprint-activated cell sorting Invited

    Kotaro Hiramatsu

    SciX2023  2023.8 

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  • Fourier-transform coherent anti-Stokes Raman scattering (FT-CARS) for biological research Invited

    Kotaro Hiramatsu

    Biomedical Raman Imaging  2023.6 

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    Fourier-transform coherent anti-Stokes Raman scattering (FT-CARS) for biological research

  • Fourier-transform coherent anti-Stokes Raman scattering (FT-CARS) for biological research Invited

    Kotaro Hiramatsu

    Biomedical Raman Imaging  2023.6 

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  • Broadband fluorescence-encoded vibrational imaging

    Phillip McCann, Kotaro Hiramatsu, Keisuke Goda

    Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, SPIE Photonics West  2023.1 

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    Event date: 2023.1 - 2023.2

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    Broadband fluorescence-encoded vibrational imaging

  • Broadband fluorescence-encoded vibrational imaging

    Phillip McCann, Kotaro Hiramatsu, Keisuke Goda

    Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, SPIE Photonics West  2023.1 

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  • High-speed FT-CARS spectroscopy for high-throughput phenotyping Invited

    Kotaro Hiramatsu

    Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, SPIE Photonics West  2023.1 

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    Event date: 2023.1 - 2023.2

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    High-speed FT-CARS spectroscopy for high-throughput phenotyping

  • High-speed FT-CARS spectroscopy for high-throughput phenotyping Invited

    Kotaro Hiramatsu

    Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, SPIE Photonics West  2023.1 

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    Event date: 2023.1 - 2023.2

    Language:English   Presentation type:Oral presentation (invited, special)  

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  • 無標識指向性進化に向けたラマン活性細胞選抜法の開発

    中尾 龍二, 平松 光太郎, 合田 圭介

    レーザ顕微鏡研究会第47回講演会・シンポジウム  2022.11 

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  • 蛍光エンコーディングによる高感度時間領域ラマン分光法

    田村 徹, McCann Phillip, 平松 光太郎, 合田 圭介

    第16回分子科学討論会2022 横浜  2022.9 

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    Event date: 2022.9

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  • Fluorescence-Encoded Time-Domain Coherent Raman Spectroscopy

    Phillip McCann, Kotaro Hiramatsu, Keisuke Goda

    International conference on Raman spectroscopy 2022  2022.8 

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    Fluorescence-Encoded Time-Domain Coherent Raman Spectroscopy

  • Super-multiplex flow cytometry by cyanine-based Raman tags

    Ryo Nishiyama, Kotaro Hiramatsu, Kosuke Dodo, Shintaro Kawamura, Mikiko Sodeoka, Keisuke Goda

    International conference on Raman spectroscopy 2022  2022.8 

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    Super-multiplex flow cytometry by cyanine-based Raman tags

  • Label-free characterization of rare-cell populations by high-throughput Raman flow cytometry

    Kotaro Hiramatsu, Matt Lindley, Koji Yamada, Kengo Suzuki, Keisuke Goda

    International conference on Raman spectroscopy 2022  2022.8 

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    Label-free characterization of rare-cell populations by high-throughput Raman flow cytometry

  • Gold nanomesh for wearable SERS

    Kotaro Hiramatsu, Limei Liu, Pablo Martinez Pancorbo, Ting-Hui Xiao, Saya Noguchi, Machiko Marumi, Julia Gala de Pablo, Siddhant Karhadkar, Hiroki Segawa, Yasutaka Kitahama, Tamitake Itoh, Junle Qu, Kuniharu Takei, Keisuke Goda

    International conference on Raman spectroscopy 2022  2022.8 

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    Gold nanomesh for wearable SERS

  • Gold nanomesh for wearable SERS

    Kotaro Hiramatsu, Limei Liu, Pablo Martinez Pancorbo, Ting-Hui Xiao, Saya Noguchi, Machiko Marumi, Julia Gala de Pablo, Siddhant Karhadkar, Hiroki Segawa, Yasutaka Kitahama, Tamitake Itoh, Junle Qu, Kuniharu Takei, Keisuke Goda

    International conference on Raman spectroscopy 2022  2022.8 

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    Gold nanomesh for wearable SERS

  • Super-multiplex flow cytometry by cyanine-based Raman tags

    Ryo Nishiyama, Kotaro Hiramatsu, Kosuke Dodo, Shintaro Kawamura, Mikiko Sodeoka, Keisuke Goda

    International conference on Raman spectroscopy 2022  2022.8 

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  • Fluorescence-Encoded Time-Domain Coherent Raman Spectroscopy

    Phillip McCann, Kotaro Hiramatsu, Keisuke Goda

    International conference on Raman spectroscopy 2022  2022.8 

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  • Gold nanomesh for wearable SERS

    Kotaro Hiramatsu, Limei Liu, Pablo Martinez Pancorbo, Ting-Hui Xiao, Saya Noguchi, Machiko Marumi, Julia Gala de Pablo, Siddhant Karhadkar, Hiroki Segawa, Yasutaka Kitahama, Tamitake Itoh, Junle Qu, Kuniharu Takei, Keisuke Goda

    International conference on Raman spectroscopy 2022  2022.8 

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    Event date: 2022.8

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  • Gold nanomesh for wearable SERS

    Kotaro Hiramatsu, Limei Liu, Pablo Martinez Pancorbo, Ting-Hui Xiao, Saya Noguchi, Machiko Marumi, Julia Gala de Pablo, Siddhant Karhadkar, Hiroki Segawa, Yasutaka Kitahama, Tamitake Itoh, Junle Qu, Kuniharu Takei, Keisuke Goda

    International conference on Raman spectroscopy 2022  2022.8 

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    Event date: 2022.8

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  • Label-free characterization of rare-cell populations by high-throughput Raman flow cytometry

    Kotaro Hiramatsu, Matt Lindley, Koji Yamada, Kengo Suzuki, Keisuke Goda

    International conference on Raman spectroscopy 2022  2022.8 

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    Event date: 2022.8

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  • Unconventional modalities in flow cytometry FLIM, Raman, SHG, etc. Invited

    Kotaro Hiramatsu

    The Complexity of Dynamics and Kinetics from Single Molecules to Cells, Telluride Science and Innovation Center  2022.6 

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    Event date: 2022.6

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    Unconventional modalities in flow cytometry FLIM, Raman, SHG, etc.

  • 偏光分解ヘテロダインCARSによるラマン光学活性の観測

    平松光太郎, 奥野将成, 加納英明, 濵口宏夫

    日本化学会第92春季年会  2012.3 

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    Observation of Raman optical activity with polarization-resolved heterodyne coherent anti-Stokes Raman scattering

  • CARS-ROAによる分子キラリティーの観測

    平松光太郎, 奥野将成, 加納英明, 永田敬, 濵口宏夫

    第6回分子科学討論会  2012.9 

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    Observation of molecular chirality by CARS-ROA

  • CARS-ROAのショットノイズ限界測定

    平松光太郎, 奥野将成, 加納英明, 永田敬, 濵口宏夫

    日本分光学会年次講演会  2012.11 

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    Shot-noise limited detection of CARS-ROA

  • Raman optical activity measured by CARS spectral interferometry International conference

    Kotaro Hiramatsu, Hideaki Kano, Takashi Nagata

    European Conference on Nonlinear Optical Spectroscopy (ECONOS) 2013  2013.4 

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    Raman optical activity measured by CARS spectral interferometry

  • Raman optical activity measured by nonlinear Raman scattering International conference

    Kotaro Hiramatsu, Hideaki Kano, Takashi Nagata

    International Conference on Chiroptical Spectroscopy 2013 (CD2013)  2013.6 

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    Raman optical activity measured by nonlinear Raman scattering

  • Raman optical activity measured by coherent anti-Stokes Raman scattering International conference

    Kotaro Hiramatsu, Hideaki Kano, Takashi Nagata

    Seventh International Conference on Advanced Vibrational Spectroscopy (ICAVS7)  2013.8 

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    Raman optical activity measured by coherent anti-Stokes Raman scattering

  • コヒーレントラマン散乱を用いたラマン光学活性分光法の開発 Invited

    平松光太郎

    第9回 若手研究者たちによる先端的レーザー分光シンポジウム  2013.12 

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    Development of Raman optical activity spectroscopy with cohernet Raman scattering

  • コヒーレントラマン分光法でみる分子のキラリティー Invited

    平松光太郎

    量子エレクトロニクス研究会「バイオ・メディカルフォトニクス:基礎と応用の最前線」  2013.12 

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    Molecular chirality probed by coherent Raman scattering

  • Coherent Raman optical activity spectroscopy Invited International conference

    Kotaro Hiramatsu

    Spectroscopic studies on molecular chirality  2013.12 

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    Coherent Raman optical activity spectroscopy

  • ヘテロダイン検出法によるアキラルバックグラウンドフリーな光活性測定法の開発 Invited

    平松光太郎

    第5回キラルサイエンス&テクノロジーシンポジウム  2014.2 

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    Development of achiral-background-free chiroptical spectroscopies with heterodyne detection

  • 広帯域・高感度フェムト秒時間分解円二色性分光法の開発

    平松光太郎, 永田 敬

    日本化学会第95春季年会  2015.3 

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    Development of broadband and sensitive femtosecond time-resolved circular dichroism spectroscopy

  • 分光干渉法を用いた広帯域高感度フェムト秒 時間分解円二色性分光法の開発

    平松光太郎, 永田 敬

    日本分光学会平成27年度年次講演会  2015.6 

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    Development of broadband and sensitive femtosecond time-resolved circular dichroism spectroscopy by using spectral interferometry

  • Visible-excited CARS-ROA spectroscopy

    Kotaro Hiramatsu, Takashi Nagata, Hideaki Kano

    15th International Conference on Chiroptical Spectroscopy  2015.8 

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    Visible-excited CARS-ROA spectroscopy

  • Broadband and ultrasensitive femtosecond time-resolved circular dichroism spectroscopy using spectral interferometry

    Kotaro Hiramatsu, Takashi Nagata

    15th International Conference on Chiroptical Spectroscopy  2015.8 

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    Broadband and ultrasensitive femtosecond time-resolved circular dichroism spectroscopy using spectral interferometry

  • 超高速円二色性分光法による生体分子の励起状態ダイナミクス観測

    平松光太郎, 永田 敬

    第9回分子科学討論会  2015.9 

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    Excited-state dynamics of biomolecules studied by time-resolved circular dichroism spectroscopy

  • フェムト秒CD分光法による超高速キラル反転の実時間観測 Invited

    平松光太郎

    「柔らかな分子系」ワークショップ 構造変化で操る分子の機能  2017.1 

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  • Rapid-Scan Fourier-Transform CARS Spectroscopy with Heterodyne Detection

    Yizhi Luo, Kotaro Hiramatsu, Takuro Ideguchi, Keisuke Goda

    日本化学会第97春季年会  2017.3 

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    Rapid-Scan Fourier-Transform CARS Spectroscopy with Heterodyne Detection

  • 超高速キラル分光法の開発と応用 Invited

    平松 光太郎

    第7回光科学異分野横断萌芽研究会  2017.8 

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    Development and application of ultrafast chiroptical spectroscopy

  • 高速フーリエ変換CARS顕微鏡

    木下川 涼, 平松 光太郎, 井手口 拓郎, 合田 圭介

    第78回応用物理学会秋季学術講演会  2017.9 

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    Rapid Fourier-transform CARS microscopy

  • コヒーレントラマン散乱を用いたROA分光法

    平松光太郎

    日本分光学会赤外ラマン部会, キラル振動分光法の基礎と最新技術  2017.11 

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    Coherent ROA spectroscopy

  • High-speed broadband Fourier-transform CARS microscopy

    Kotaro Hiramatsu, Ryo Kinegawa, Kazuki Hashimoto, Venkata Ramaiah Badarla, Takuro Ideguchi, Keisuke Goda

    SPIE Photonics West, High-Speed Biomedical Imaging and Spectroscopy III: Toward Big Data Instrumentation and Management  2018.1 

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    High-speed broadband Fourier-transform CARS microscopy

  • Heterodyne-detected rapid-scan Fourier-transform CARS spectroscopy

    Kotaro Hiramatsu, Yizhi Luo, Takuro Ideguchi, Keisuke Goda

    SPIE Photonics West, Biomedical Vibrational Spectroscopy 2018: Advances in Research and Industry  2018.1 

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    Heterodyne-detected rapid-scan Fourier-transform CARS spectroscopy

  • High-speed Fourier-transform coherent anti-Stokes Raman scattering spectroscopy with heterodyne detection

    Kotaro Hiramatsu, Yizhi Luo, Takuro Ideguchi, Keisuke Goda

    日本化学会第98春季年会  2018.3 

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    High-speed Fourier-transform coherent anti-Stokes Raman scattering spectroscopy with heterodyne detection

  • Simultaneous FT-CARS and fluorescence spectroscopy at 24,000 spectra per second

    Matthew Lindley, Hayate Nomoto, Kotaro Hiramatsu, Keisuke Goda

    日本分光学会年次講演会  2018.5 

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    Simultaneous FT-CARS and fluorescence spectroscopy at 24,000 spectra per second

  • 時間領域Raman分光法の生命科学研究への応用

    平松 光太郎, 合田 圭介

    第8回光科学異分野横断萌芽研究会  2018.8 

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    Time-domain Raman spectroscopy and its application to biological research

  • デュアルコムコヒーレントラマン分光によるマイクロ流体1粒子分析

    滝沢 繁和, 中村 將, 井手口 拓郎, 玉光 未侑, 李 相旭, 平松 光太郎, Ramaiah Venkata, 朴 智雄, 笠井 宥佑, 早川 健, 佐久間 臣耶, 新井 史人, 合田 圭介

    第79回応用物理学会秋季学術講演会  2018.9 

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    Microfluidic particle analysis with dual-comb coherent Raman spectroscopy

  • High-speed simultaneous Raman-fluorescence spectrometer

    Matthew Lindley, Hayate Nomoto, Kotaro Hiramatsu, Keisuke Goda

    第79回応用物理学会秋季学術講演会  2018.9 

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    High-speed simultaneous Raman-fluorescence spectrometer

  • High-throughput broadband CARS flow cytometry at >2,000 cells/s International conference

    Kotaro Hiramatsu, Takuro Ideguchi, Yusuke Yonamine, SangWook Lee, Yizhi Luo, Kazuki Hashimoto, Takuro Ito, Misa Hase, Jee-Woong Park, Yusuke Kasai, Shinya Sakuma, Takeshi Hayakawa, Fumihito Arai, Yu Hoshino, Keisuke Goda

    SPIE Photonics West, High-Speed Biomedical Imaging and Spectroscopy IV: Toward Big Data Instrumentation and Management, Photonics West  2019.2 

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    Country:United States  

    High-throughput broadband CARS flow cytometry at >2,000 cells/s

  • High-speed simultaneous CARS and fluorescence spectroscopies International conference

    Matthew Lindley, Hayate Nomoto, Kotaro Hiramatsu, Keisuke Goda

    SPIE Photonics West, High-Speed Biomedical Imaging and Spectroscopy IV: Toward Big Data Instrumentation and Management, Photonics West  2019.2 

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    High-speed simultaneous CARS and fluorescence spectroscopies

  • コヒーレントラマン散乱を用いたROA分光法 Invited

    平松 光太郎

    第23回HiSOR研究会 ~分子キラリティの計測・理論技術の革新から迫る生命機能研究の新展開~  2019.3 

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    Coherent ROA spectroscopy

  • Intelligent Image-Activated Cell Sorting: Principles and Applications

    平松 光太郎, 新田 尚, 杉村 武昭, 磯崎 瑛宏, 三上 秀治, Dino Di Carlo, 細川 陽一郎, 上村 想太郎, 小関 泰之, 合田 圭介

    日本化学会第99春季年会2019  2019.3 

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    Intelligent Image-Activated Cell Sorting: Principles and Applications

  • High-throughput broadband Raman flow cytometry

    平松光太郎, 井手口 拓郎, 与那嶺雄介, Lee SangWook, Yizhi Luo, 橋本 和樹, 伊藤 卓朗, 長谷 美佐, Jee-Woong Park, 笠井 宥佑, 佐久間 臣耶, 早川 健, 新井 史人, 星野 友, 合田圭介

    日本化学会第99春季年会2019  2019.3 

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    Country:Japan  

    High-throughput broadband Raman flow cytometry

  • Label-free molecularly specific flow cytometry and beyond International conference

    Kotaro Hiramatsu, Yusuke Yonamine, Takuro Ito, Yu Hoshino, Keisuke Goda

    34th Congress of the International Society for Advancement of Cytometry  2019.6 

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    Language:English  

    Country:Canada  

    Label-free molecularly specific flow cytometry and beyond

  • High-throughput Raman spectroscopic flow cytometry" Invited International conference

    Kotaro Hiramatsu, Keisuke Goda

    The Australia and New Zealand Nano and Microfluidics 2019  2019.7 

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    Country:Australia  

    High-throughput Raman spectroscopic flow cytometry"

  • Coherent Raman spectroscopic flow cytometry for large-scale single cell analysis International conference

    Kotaro Hiramatsu, Yusuke Yonamine, Takuro Ito, Yu Hoshino, Keisuke Goda

    International conference on vibrational spectroscopy  2019.7 

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    Coherent Raman spectroscopic flow cytometry for large-scale single cell analysis

  • 高速ラマン分光フローサイトメトリー

    平松 光太郎, 合田圭介

    第9回光科学異分野横断萌芽研究会  2019.8 

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    Language:Japanese  

    Country:Japan  

  • 振動分光フローサイトメトリーによる大規模無標識1細胞解析

    平松 光太郎, Matthew Lindley, 山田 康嗣, 鈴木 健吾, 合田 圭介

    第80回応用物理学会秋季学術講演会  2019.9 

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    Country:Japan  

  • 圧縮センシングを用いた時間領域コヒーレントラマン分光

    滝沢 繁和, 平松 光太郎, 合田 圭介

    第80回応用物理学会秋季学術講演会  2019.9 

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    Country:Other  

  • Simultaneous Raman-Fluorescence Flow Cytometry

    Matthew Lindley, Kotaro Hiramatsu, Fukashi Shibata, Tsuyoshi Takeshita, Shigeyuki Kawano, Keisuke Goda

    第80回応用物理学会秋季学術講演会  2019.9 

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    Country:Japan  

    Simultaneous Raman-Fluorescence Flow Cytometry

  • 高速ラマン分光法による大規模一細胞解析 Invited

    平松 光太郎

    第17回医用分光学研究会  2019.11 

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    Country:Japan  

    Large-scale single-cell analysis by high-speed Raman spectroscopy

  • Large-scale single-cell analysis by ultra-rapid time-domain Raman spectroscopy Invited International conference

    Kotaro Hiramatsu, Keisuke Goda

    The 12th International Photonics and OptoElectronics Meetings  2019.11 

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    Language:English  

    Country:China  

    Large-scale single-cell analysis by ultra-rapid time-domain Raman spectroscopy

  • High-throughput Raman flow cytometry Invited International conference

    Kotaro Hiramatsu, Keisuke Goda

    SelectBio 2019 - Microfluidics & Organ-on-a-Chip Asia 2019  2019.11 

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    Country:Japan  

    High-throughput Raman flow cytometry

  • High-throughput Broadband Vibrational Flow Cytometry International conference

    Kotaro Hiramatsu, Keisuke Goda

    Biomedical Raman Imaging  2019.11 

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    Country:Japan  

    High-throughput Broadband Vibrational Flow Cytometry

  • 多元的分光計測による大規模一細胞解析 Invited

    平松 光太郎

    分光学会生細胞分光部会シンポジウム  2019.12 

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    Multiplex spectroscopy for large-scale single-cell analysis

  • 高スループット振動分光フローサイトメトリー Invited

    平松 光太郎, 合田 圭介

    レーザー学会 学術講演会 第40回年次大会  2020.1 

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    Country:Japan  

    High-throughput vibrational spectroscopic flow cytometry

  • Highly sensitive low-frequency Raman spectroscopy enabled by Sagnac-enhanced impulsive stimulated Raman scattering International conference

    Walker Peterson, Kotaro Hiramatsu, Keisuke Goda

    SPIE Photonics West, Label-free Biomedical Imaging and Sensing (LBIS) 2020  2020.2 

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    Highly sensitive low-frequency Raman spectroscopy enabled by Sagnac-enhanced impulsive stimulated Raman scattering

  • High-throughput vibrational flow cytometry Invited International conference

    Kotaro Hiramatsu, Keisuke Goda

    Advanced Chemical Microscopy for Life Science and Translational Medicine, Photonics West  2020.2 

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    Country:United States  

    High-throughput vibrational flow cytometry

  • High-throughput multimodal Raman-fluorescence flow cytometry International conference

    Matthew Lindley, Kotaro Hiramatsu, Fukashi Shibata, Tsuyoshi Takeshita, Shigeyuki Kawano, Keisuke Goda

    SPIE Photonics West, High-Speed Biomedical Imaging and Spectroscopy V  2020.2 

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    Country:United States  

    High-throughput multimodal Raman-fluorescence flow cytometry

  • 繰り返し周波数変調による高速コヒーレントラマン分光法

    亀山 理紗子, 平松 光太郎, 合田 圭介

    分子科学会オンライン討論会  2020.9 

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    Country:Japan  

  • ラベルフリー代謝工学に向けたラマンフローサイトメーターの開発

    中尾 龍二, 平松 光太郎, 橋爪里奈, 那須 雄介, Robert E. Campbell, 合田 圭介

    量子生命科学会 第二回大会  2020.12 

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  • 擬デュアルコム光源による高速コヒーレントラマン分光法

    亀山 理紗, 滝沢 繁和, 平松 光太郎, 合田 圭介

    第68回応用物理学会春季学術講演会  2021.3 

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    Country:Japan  

  • High-speed low-frequency vibrational spectroscopy using a Sagnac interferometer

    Jorgen Walker Peterson, Matt Lindley, Julia Gala de Pablo, Kotaro Hiramatsu, Keisuke Goda

    第68回応用物理学会春季学術講演会  2021.3 

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    Country:Japan  

    High-speed low-frequency vibrational spectroscopy using a Sagnac interferometer

  • Fluorescence-encoded time-domain coherent Raman spectroscopy through single-photon counting International conference

    Phillip Charles McCann, Kotaro Hiramatsu, Keisuke Goda

    Single Molecule Spectroscopy and Superresolution Imaging XIV, Photonics West  2021.3 

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    Fluorescence-encoded time-domain coherent Raman spectroscopy through single-photon counting

  • Fast, sensitive dual-comb CARS spectroscopy with a quasi-dual-comb laser International conference

    Risako Kameyama, Sigekazu Takizawa, Kotaro Hiramatsu, Keisuke Goda

    High-Speed Biomedical Imaging and Spectroscopy VI, Photonics West  2021.3 

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    Fast, sensitive dual-comb CARS spectroscopy with a quasi-dual-comb laser

  • 四波混合・第二次高調波イメージングフローサイトメトリー

    木下川 涼, ガラ デ パブロ ジュリア, 王 弋, 平松 光太郎, 合田 圭介

    第82回応用物理学会秋季学術講演会  2021.9 

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  • 圧縮フーリエ分光イメージング:シミュレーションによる検討

    滝沢繁和, 平松 光太郎, 合田圭介

    2021年度日本分光学会年次講演会  2021.10 

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  • プローブ光の位相遅延と周波数シフトの同期検出による低波数領域と指紋領域の超高速インパルシブ・ラマン分光法

    ピーターソン ウォーカー, ガラデパブロー, ジュリア, リンドリー マット, 平松 光太郎, 合田 圭介

    2021年度日本分光学会年次講演会  2021.10 

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  • プラズモンフリーSERSのための多孔質カーボンナノワイヤー

    平松 光太郎, 陳南, 肖廷輝, 罗桢埸, 北濱康孝, 岸本直樹, 伊藤民武, 程振洲, 合田圭介, 合田圭介

    2021年度日本分光学会年次講演会  2021.10 

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  • シリコンナノディスクアレイを用いたキラル場増強ラマン光学活性

    平松 光太郎, 肖廷, 罗桢埸, 磯崎瑛宏, 伊藤民武, 程振洲, 野村政宏, 岩本敏, 合田圭介

    2021年度日本分光学会年次講演会  2021.10 

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  • Raman spectroscopy and imaging of single cells in flow Invited

    Kotaro Hiramatsu, Keisuke Goda

    SciX2021  2021.10 

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    Raman spectroscopy and imaging of single cells in flow

  • Fluorescence-Encoded Time-Domain Coherent Raman Spectroscopy

    Phillip McCann, Kotaro Hiramatsu, Keisuke Goda

    2021年度日本分光学会年次講演会  2021.10 

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    Fluorescence-Encoded Time-Domain Coherent Raman Spectroscopy

  • 高スループットラマンフローサイトメーターによる多細胞分析 Invited

    平松 光太郎

    レーザ顕微鏡研究会第46回講演会  2021.11 

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    Country:Other  

    Multi-cell analysis by high-throughput Raman flow cytometry

  • 蛍光エンコーディングによる超高感度ラマン分光計測 Invited

    平松 光太郎

    レーザー学会学術講演会第42回年次大会  2022.1 

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    Super-sensitive Raman spectroscopy by fluorescence encoding

  • THz-fingerprint coherent Raman spectroscopy at over 20,000 spectra/sec

    Jorgen Walker Peterson, Matt Lindley, Julia Gala de Pablo, Kotaro Hiramatsu, Keisuke Goda

    High-Speed Biomedical Imaging and Spectroscopy VII, SPIE Photonics West 2022  2022.1 

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    THz-fingerprint coherent Raman spectroscopy at over 20,000 spectra/sec

  • Raman image-activated cell sorting and beyond Invited

    Kotaro Hiramatsu, Keisuke Goda

    Advanced Chemical Microscopy for Life Science and Translational Medicine 2022  2022.1 

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    Raman image-activated cell sorting and beyond

  • Raman flow cytometry on a chip Invited

    Kotaro Hiramatsu

    High-Speed Biomedical Imaging and Spectroscopy VII, SPIE Photonics West  2022.1 

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    Raman flow cytometry on a chip

  • Porous carbon nanowires for plasmon-free SERS

    Tinghui Xiao, Nan Chen, Zhengyi Luo, Yasutaka Kitahama, Kotaro Hiramatsu, Naoki Kishimoto, Tamitake Itoh, Zhenzhou Cheng, Keisuke Goda

    Biomedical Vibrational Spectroscopy 2022: Advances in Research and Industry, SPIE Photonics West 2022  2022.1 

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    Porous carbon nanowires for plasmon-free SERS

  • High-speed Fourier-transform spectroscopy by non-mechanical cavity modulation Invited

    Kotaro Hiramatsu

    Smart Photonic and Optoelectronic Integrated Circuits 2022, SPIE Photonics Wes  2022.1 

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    High-speed Fourier-transform spectroscopy by non-mechanical cavity modulation

  • Chiral-field-enhanced Raman optical activity by a silicon nanodisk array

    Tinghui Xiao, Zhengyi Luo, Kotaro Hiramatsu, Akihiro Isozaki, Tamitake Itoh, Zhenzhou Cheng, Masahiro Nomura, Satoshi Iwamoto, Keisuke Goda

    Biomedical Vibrational Spectroscopy 2022: Advances in Research and Industry, SPIE Photonics West 2022  2022.1 

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    Chiral-field-enhanced Raman optical activity by a silicon nanodisk array

  • 切って、置いて、測るだけ:金ナノメッシュによる超簡便SERS

    北濱 康孝, 合田 圭介, 丸見 真智子, Pancorbo Pablo, 瀬川 尋貴, Xiao Ting-Hui, 平松 光太郎, Yang William

    第70回応用物理学会春季学術講演会  2022.3 

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  • Ultra-broadband time-domain Raman spectroscopy using synchronized mode-locked lasers Invited

    Kotaro Hiramatsu, Keisuke Goda

    Biomedical Spectroscopy, Microscopy, and Imaging II, Photonics Europe  2022.4 

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    Ultra-broadband time-domain Raman spectroscopy using synchronized mode-locked lasers

  • 環状分子を用いた超高輝度ラマンプローブの開発

    古屋 圭惟, 西山 諒, McCann Phillip, Kacenauskaite Laura, Laursen Bo, H. Flood Ama, 平松 光太郎, 合田 圭介

    第70回応用物理学会春季学術講演会  2023.3 

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  • ラマンタグを用いた超多色フローサイトメトリー

    西山 諒, 平松 光太郎, 河村 伸太郎, 闐闐 孝介, 古屋 圭惟, ミン ウェイ, 袖岡 幹子, 合田 圭介

    第70回応用物理学会春季学術講演会  2023.3 

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  • 無標識分光計測に基づく細胞分取 Invited

    平松 光太郎

    第71回応用物理学会春季学術講演会  2024.3 

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    Country:Other  

  • 時間領域ラマン分光法の深化と生物応用 Invited

    平松 光太郎

    日本光学会年次学術講演会  2023.11 

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    Country:Other  

  • Chiral-field-enhanced Raman optical activity by a silicon nanodisk array

    Tinghui Xiao, Zhengyi Luo, Kotaro Hiramatsu, Akihiro Isozaki, Tamitake Itoh, Zhenzhou Cheng, Masahiro Nomura, Satoshi Iwamoto, Keisuke Goda

    Biomedical Vibrational Spectroscopy 2022: Advances in Research and Industry, SPIE Photonics West 2022  2022.1 

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  • Porous carbon nanowires for plasmon-free SERS

    Tinghui Xiao, Nan Chen, Zhengyi Luo, Yasutaka Kitahama, Kotaro Hiramatsu, Naoki Kishimoto, Tamitake Itoh, Zhenzhou Cheng, Keisuke Goda

    Biomedical Vibrational Spectroscopy 2022: Advances in Research and Industry, SPIE Photonics West 2022  2022.1 

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  • Raman flow cytometry on a chip Invited

    Kotaro Hiramatsu

    High-Speed Biomedical Imaging and Spectroscopy VII, SPIE Photonics West  2022.1 

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  • Raman image-activated cell sorting and beyond Invited

    Kotaro Hiramatsu, Keisuke Goda

    Advanced Chemical Microscopy for Life Science and Translational Medicine 2022  2022.1 

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  • THz-fingerprint coherent Raman spectroscopy at over 20,000 spectra/sec

    Jorgen Walker Peterson, Matt Lindley, Julia Gala de Pablo, Kotaro Hiramatsu, Keisuke Goda

    High-Speed Biomedical Imaging and Spectroscopy VII, SPIE Photonics West 2022  2022.1 

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  • Ultra-broadband time-domain Raman spectroscopy using synchronized mode-locked lasers Invited

    Kotaro Hiramatsu, Keisuke Goda

    Biomedical Spectroscopy, Microscopy, and Imaging II, Photonics Europe  2022.4 

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  • ラマンタグを用いた超多色フローサイトメトリー

    西山 諒, 平松 光太郎, 河村 伸太郎, 闐闐 孝介, 古屋 圭惟, ミン ウェイ, 袖岡 幹子, 合田 圭介

    第70回応用物理学会春季学術講演会  2023.3 

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MISC

  • ラマン分光フローサイトメトリ Reviewed

    西山 諒, 古屋 圭惟, 平松 光太郎, 合田 圭介

    生物物理   2024.4

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  • ラマン分光フローサイトメトリー

    西山 諒, 古屋 圭惟, 平松 光太郎, 合田 圭介

    生物物理   64 ( 2 )   104 - 107   2024.3   ISSN:0582-4052

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    Language:Japanese   Publisher:(一社)日本生物物理学会  

  • 圧縮センシングによる高速時間領域ハイパースペクトルイメージング Reviewed

    滝沢 繁和, 平松光太郎, 小野 峻佑, 合田 圭介

    光学   2023.10

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  • ラマンイメージング x 圧縮センシング Reviewed

    平松光太郎, 滝沢繁和, 小野峻佑, 合田圭介

    分光研究   2023.8

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  • ラマンイメージング x 圧縮センシング Invited Reviewed

    平松光太郎, 滝沢繁和, 小野峻佑, 合田圭介

    分光研究   72 ( 4 )   163   2023.8

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    Authorship:Lead author, Corresponding author  

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  • 単一細胞レベルの大規模画像プロファイリングによるCOVID-19患者血液中の循環血小板凝集塊の解析—Analyzing circulating platelet aggregates in patients with COVID-19 by massive image-based single-cell profiling

    菅野 寛志, Yuqi ZHOU, 西川 真子, Ting-Hui XIAO, 鈴木 拓真, 伊林 侑真, Jeffrey HARMON, 滝沢 繁和, 平松 光太郎, 新田 尚, 亀山 理紗子, Walker PETERSON, 滝口 純, Mohammad SHIFAT-E-RABII, Yan ZHUANG, Xuwang YIN, Abu Hasnat Mohammad RUBAIYAT, Yunjie DENG, Hongqian ZHANG, 宮田 茂樹, Gustavo K. ROHDE, 岩崎 渉, 矢冨 裕, 合田 圭介

    化学とマイクロ・ナノシステム = Journal of the Society for Chemistry and Micro-Nano Systems : 化学とマイクロ・ナノシステム研究会誌   2022.3

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    Language:Japanese  

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Industrial property rights

Patent   Number of applications: 0   Number of registrations: 0
Utility model   Number of applications: 0   Number of registrations: 0
Design   Number of applications: 0   Number of registrations: 0
Trademark   Number of applications: 0   Number of registrations: 0

Professional Memberships

  • THe Chemical Society of Japan

  • Japan Society for Molecular Science

  • The Spectroscopical Society of Japan

  • The Japan Society of Applied Physics

  • The Optical Society of Japan

  • American Chemical Society

  • SPIE - The international society of optics and photonics

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Committee Memberships

  • 日本光学会   光・光委員会   Domestic

    2023.4 - Present   

  • 日本分光学会   出版委員   Domestic

    2022.4 - Present   

Academic Activities

  • Screening of academic papers

    Role(s): Peer review

    2023

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:7

  • Screening of academic papers

    Role(s): Peer review

    2022

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:6

  • Screening of academic papers

    Role(s): Peer review

    2022

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:8

  • 事務局長

    量子生命科学研究会第3回大会  ( Japan ) 2021.9

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    Type:Competition, symposium, etc. 

    Number of participants:174

  • Screening of academic papers

    Role(s): Peer review

    2020

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:17

  • Screening of academic papers

    Role(s): Peer review

    2019

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:4

  • Screening of academic papers

    Role(s): Peer review

    2019

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:6

  • Screening of academic papers

    Role(s): Peer review

    2017

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:7

  • Screening of academic papers

    Role(s): Peer review

    2016

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:4

  • Screening of academic papers

    Role(s): Peer review

    2014

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:1

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Research Projects

  • 高速超解像イメージングのための空間―周波数圧縮顕微鏡の開発

    2024

    コニカミノルタ画像科学奨励賞

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    Authorship:Principal investigator  Grant type:On-campus funds, funds, etc.

  • コグニティブ分光プラットフォームの創生

    2022.4 - 2028.3

    科学技術振興機構 

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    Authorship:Principal investigator 

  • テラヘルツ領域の1分子振動分光イメージング

    2022.4 - 2026.3

    日本学術振興会 

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    Authorship:Principal investigator 

  • コグニティブ分光プラットフォームの創生

    2022 - 2028

    JST Strategic Basic Research Program (Ministry of Education, Culture, Sports, Science and Technology)

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    Authorship:Principal investigator  Grant type:Contract research

  • テラヘルツ領域の1分子振動分光イメージング

    Grant number:22H02029  2022 - 2025

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 蛍光エンコーディングによる1分子振動分光イメージング

    2022 - 2023

    光科学技術研究振興財団

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    Grant type:On-campus funds, funds, etc.

  • 蛍光エンコーディングによる超高感度ラマン分光

    Grant number:20K15227  2020 - 2021

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Early-Career Scientists

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 任意のスペクトル次元を測定できるfunctional Raman分光法の開発

    2018 - 2021

    JST Strategic Basic Research Program (Ministry of Education, Culture, Sports, Science and Technology)

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    Authorship:Principal investigator  Grant type:Contract research

  • 高性能ラマン・フローサイトメトリーを用いた無標識代謝解析によるスマートセル選抜技術の研究開発

    2018

    NEDO 植物等の生物を用いた高機能品生産技術の開発/ スマートセル関連技術の社会実装推進に向けて解決すべき新規課題の検討

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    Authorship:Coinvestigator(s)  Grant type:Contract research

  • 2光子コヒーレンストモグラフィー法の開発

    2018

    立石科学技術振興財団 研究助成(A)

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    Authorship:Principal investigator  Grant type:On-campus funds, funds, etc.

  • 光周波数コムを用いた磁気共鳴測定法の開発とバイオイメージングへの応用

    Grant number:17K19099  2017 - 2019

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Challenging Research(Exploratory)

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    Grant type:Scientific research funding

  • 光周波数コムを用いたOptical NMR法の開発

    2017

    公益財団法人精密測定技術振興財団 研究助成

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    Authorship:Principal investigator  Grant type:On-campus funds, funds, etc.

  • Optical NMR法の開発とそれによる細胞内分子の可視化

    2017

    公益財団法人宇部興産学術振興財団 第57回学術奨励賞

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    Authorship:Principal investigator  Grant type:On-campus funds, funds, etc.

  • コヒーレントラマン分光法による時間分解ラマン光学活性測定法の開発

    2013 - 2016

    Japan Society for the Promotion of Science  Research Fellowships for Young Scientists

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    Authorship:Principal investigator  Grant type:Joint research

  • コヒーレントラマン分光法による時間分解ラマン光学活性測定法の開発

    Grant number:13J01123  2013 - 2015

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for JSPS Fellows

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    Grant type:Scientific research funding

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Educational Activities

  • Our goal is to create new methodologies to capture natural phenomena, to carry out research that can create new value through such research, and to provide education that will foster human resources with the ability to do so. To this end, our educational activities focus on the following elements
    1. Deep understanding of basic physics and chemistry and the ability to apply them on one's own
    2. the ability to coarse-grained and model phenomena that cannot be fully explained by deduction from basic equations
    3. strong curiosity about natural phenomena
    4. matching curiosity with social needs
    While the amount of data obtained experimentally has dramatically increased due to improvements in the performance of measuring instruments and computers, and the estimation of phenomena by machine learning has become very powerful in basic science research, it is still difficult to extrapolate to phenomena that have not been measured before or to areas where data is scarce. In order to explore such new areas, we train researchers to define an area based on their curiosity, design measurements that can cover the area based on basic physics and chemistry, and then measure and model the area.

Class subject

  • 光生物物理化学(※コロイド化学)

    2024.10 - 2025.3   Second semester

  • 化学数学

    2024.4 - 2024.9   First semester

Other educational activity and Special note

  • 2024  Class Teacher  学部

  • 2024  Special Affairs  博士課程に1名の学生が所属 1名の特別研究派遣学生(博士課程)を受け入れ

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    博士課程に1名の学生が所属
    1名の特別研究派遣学生(博士課程)を受け入れ

  • 2023  Class Teacher  学部

Outline of Social Contribution and International Cooperation activities

  • Public lectures are held for the general public. Accepting trainees from abroad in the laboratory.

Social Activities

  • コヒーレントラマン分光法の基礎と生命科学応用

    (株)オプトロニクス  パシフィコ横浜  2024.5

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

    コヒーレントラマンイメージングは,細胞内分子を無標識かつ高速に計測できるため,2010年頃から多くの注目を集めている.近年では市販のコヒーレントラマン顕微鏡も登場し,生命科学・医学研究において実用できるツールとなりつつある.一方,最近5年程度の先端研究としては,ラマンタグとの融合による超多色計測,蛍光エンコーディングによる高感度化,オミクス解析との融合,量子光学の応用による高感度化,フローサイトメトリーとの融合による大規模細胞解析などが挙げられ,更なる高感度化や新たな応用開拓が進められている.本講演では,コヒーレントラマン散乱の基礎を解説した後に,これら最近のトピックを紹介し,コヒーレントラマン分光法を用いた生命科学研究の今後を展望することを目指す.

  • 2023年ノーベル賞受賞研究解説会

    ピカリかがく  九大伊都 蔦屋書店  2023.11

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

Acceptance of Foreign Researchers, etc.

  • University of Limoges

    Acceptance period: 2024.5 - 2024.8   (Period):1 month or more

    Nationality:France

    Business entity:Other

Travel Abroad

  • 2014.10 - 2015.3

    Staying countory name 1:Japan   Staying institution name 1:サウサンプトン大学