KINOSHITA MASANAO
Organization
Faculty of Science Department of Chemistry Assistant Professor
School of Sciences Department of Chemistry（Joint Appointment）
Graduate School of Sciences Department of Chemistry（Joint Appointment）
Title
Assistant Professor
Profile
担当授業
H27年度前期「分析化学特論II」「化学序説」「卒業研究指導」
H27年度後期 「分析化学実験(基本操作法・データ解析・分光実験)」「卒業研究指導」
H28年度前期 「卒業研究指導」
H28年度後期 「分析化学実験(基本操作法・データ解析・分光実験)」「卒業研究指導」
H29年度前期「分析化学特論II」「化学序説」「卒業研究指導」
H29年度後期 「分析化学実験(基本操作法・データ解析・分光実験)」「卒業研究指導」
H30年度前期 「卒業研究指導」
H30年度後期 「分析化学実験(基本操作法・データ解析・分光実験)」「卒業研究指導」
H31年度前期「分析化学特論II」「卒業研究指導」
H31年度後期 「分析化学実験(基本操作法・データ解析・分光実験)」「卒業研究指導」
R2年度後期 「分析化学実験(基本操作法・データ解析・分光実験)」「卒業研究指導」
R3年度前期 「分析化学特論II」「卒業研究指導」
R3年度後期 「分析化学実験(基本操作法・分光実験)」「卒業研究指導」
R4年度前期 「卒業研究指導」
R4年度後期 「分析化学実験(基本操作法・分光実験)」「卒業研究指導」
External link
Degree
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Science
Research History
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関西学院大学理学研究院リサーチアシスタント 大阪大学理学研究院JST-ERATO村田脂質活性構造プロジェクト
Research Interests・Research Keywords
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Research theme：membrane structures and properties
Keyword：lipid rafts
Research period： 2019.6
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Research theme：Influence of local anesthetics on cell membranes
Keyword：local anesthetics
Research period： 2017.6 - 2022.6
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Research theme：membrane sructures, properties and dynamics
Keyword：membrane sructures, properties, dynamics
Research period： 2015.10
Papers
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Raft-based sphingomyelin interactions revealed by new fluorescent sphingomyelin analogs Reviewed
Masanao Kinoshita, Kenichi G. N. Suzuki, Nobuaki Matsumori, Misa Takada, Hikaru Ano, Kenichi Morigaki, Mitsuhiro Abe, Asami Makino, Toshihide Kobayashi, Koichiro M. Hirosawa, Takahiro K. Fujiwara, Akihiro Kusumi, Michio Murata
JOURNAL OF CELL BIOLOGY 216 ( 4 ) 1183 - 1204 2017.4
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Formation of a tight complex between amphidinol 3 and sterols in lipid bilayers revealed by short-range energy transfer Reviewed
Manami Hieda, Koya Tsujimura, Masanao Kinoshita, Nobuaki Matsumori
Bulletin of the Chemical Society of Japan 2022.11
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Impact of sphingomyelin acyl chain heterogeneity upon properties of raft-like membranes Reviewed
Kana Hirano, Masanao Kinoshita, Nobuaki Matsumori
Biochim. Biophys. Acta -Biomembr. 2022.8
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Behavior of Triterpenoid Saponin Ginsenoside Rh2 in Ordered and Disordered Phases in Model Membranes Consisting of Sphingomyelin, Phosphatidylcholine, and Cholesterol Reviewed
Darcy Lacanilao Garza, Shinya Hanashima, Yuichi Umegawa, Michio Murata, Masanao Kinoshita, Nobuaki Matsumori, Peter Greimel
Langmuir 2022.8
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Inimitable Impacts of Ceramides on Lipid Rafts Formed in Artificial and Natural Cell Membranes
Masanao Kinoshita, Nobuaki Matsumori
Membranes 12 ( 8 ) 727 - 727 2022.7
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Molecular substructure of the liquid-ordered phase formed by sphingomyelin and cholesterol: sphingomyelin clusters forming nano-subdomains are a characteristic feature Reviewed
Michio Murata, Nobuaki Matsumori, Masanao Kinoshita, Erwin London
Biophysical Reviews 14 ( 3 ) 655 - 678 2022.6
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脂質ラフトをターゲットとする局所麻酔薬の作用機序 Invited
@木下祥尚、@松森信明
2021.12
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Preparation of nitrogen analogs of ceramide and studies of theiraggregation in sphingomyelin bilayers Reviewed International journal
#Yasuda Hiroyuki, @Torikai Kouhei, @Kinoshita Masanao, Sazzad MA, #Tsujimura Kouya, Slotte J. Peter, @Matsumori Nobuaki.
2021.10
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Amphidinol 3 preferentially binds to cholesterol in disordered domains and disrupts membrane phase separation Reviewed International journal
Manami Hieda, Akira Sorada, Masanao Kinoshita, Nobuaki Matsumori
Biochemistry and Biophysics Reports 26 100941 - 100941 2021.7
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Metal complex lipids for fluid–fluid phase separation in co-assembled phospholipid membranes Reviewed International journal
2021.3
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Metal complex lipids for fluid-fluid phase separation in co-assembled phospholipid membranes. Reviewed International journal
Ryo Ohtani, Yuka Anegawa, Hikaru Watanabe, Yutaro Tajima, Masanao Kinoshita, Nobuaki Matsumori, Kenichi Kawano, Saeko Yanaka, Koichi Kato, Masaaki Nakamura, Masaaki Ohba, Shinya Hayami
Angewandte Chemie (International ed. in English) 60 ( 24 ) 13603 - 13608 2021.3
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Amphidinol 3 preferentially binds to cholesterol in disordered domains and disrupts membrane phase separation. Reviewed International journal
#Manami Hieda, #Akira Sorada, @Masanao Kinoshita, @Nobuaki Matsumori
Biochim. Biophys. Rep. 2021.2
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Low-flux scanning electron diffraction reveals substructures inside the ordered membrane domain Reviewed
Masanao Kinoshita, Simpei Yamaguchi, Nobuaki Matsumori
Scientific Reports 2020.12
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β-Glucosylation of cholesterol reduces sterol-sphingomyelin interactions Reviewed
183496 - 183496 2020.10
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New Fluorescent Sphingomyelin Probes Reveal Inter–lipid Interactions and Mechanism for Lipid–raft Formation Reviewed
45 ( 5 ) 221 - 255 2020.9
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Assembly formation of minor dihydrosphingomyelin in sphingomyelin-rich ordered membrane domains Reviewed
Masanao Kinoshita, Takumi Kyo, Nobuaki Matsumori
Scientific Reports 10 ( 1 ) 2020.7
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Pseudo‐membrane jackets: Two‐dimensional coordination polymers achieving visible phase separation in cell membrane Reviewed
2020.6
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Archaeal Glycolipid S-TGA-1 Is Crucial for Trimer Formation and Photocycle Activity of Bacteriorhodopsin. Reviewed International journal
Masataka Inada, Masanao Kinoshita, Nobuaki Matsumori
ACS chemical biology 15 ( 1 ) 197 - 204 2020.1
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The influence of ceramide and its dihydro analog on the physico-chemical properties of sphingomyelin bilayers Reviewed
Masanao Kinoshita, Kaoru Tanaka, Nobuaki Matsumori
Chemistry and Physics of Lipids 226 104835 - 104835 2020.1
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Defining raft domains in the plasma membrane Reviewed
Traffic 21 ( 1 ) 106 - 137 2019.11
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Mechanism of local anesthetic-induced disruption of raft-like ordered membrane domains Reviewed
Masanao Kinoshita, Takeshi Chitose, Nobuaki Matsumori
Biochimica et Biophysica Acta (BBA) - General Subjects 1863 ( 9 ) 1381 - 1389 2019.9
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A concise method for quantitative analysis of interactions between lipids and membrane proteins Reviewed
Masataka Inada, Masanao Kinoshita, Ayumi Sumino, Shigetoshi Oiki, Nobuaki Matsumori
Analytica Chimica Acta 1059 103 - 112 2019.6
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Preparation and Membrane Distribution of Fluorescent Derivatives of Ceramide
Takaaki Matsufuji, Masanao Kinoshita, Nobuaki Matsumori
Langmuir 35 ( 6 ) 2392 - 2398 2019.2
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Theonellamide A, a marine-sponge-derived bicyclic peptide, binds to cholesterol in aqueous DMSO: Solution NMR-based analysis of peptide-sterol interactions using hydroxylated sterol Reviewed
Kimberly Cornelio, Rafael Atillo Espiritu, Shinya Hanashima, Yasuto Todokoro, Raymond Malabed, Masanao Kinoshita, Nobuaki Matsumori, Michio Murata, Shinichi Nishimura, Hideaki Kakeya, Minoru Yoshida, Shigeki Matsunaga
Biochimica et Biophysica Acta (BBA) - Biomembranes 1861 ( 1 ) 228 - 235 2019.1
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On the Importance of the C(1)–OH and C(3)–OH Functional Groups of the Long-Chain Base of Ceramide for Interlipid Interaction and Lateral Segregation into Ceramide-Rich Domains Reviewed
Anna Möuts, Elina Vattulainen, Takaaki Matsufuji, Masanao Kinoshita, Nobuaki Matsumori, J. Peter Slotte
34 ( 51 ) 15864 - 15870 2018.12
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Sphingomyelin Stereoisomers Reveal That Homophilic Interactions Cause Nanodomain Formation Reviewed
Yo Yano, Shinya Hanashima, Tomokazu Yasuda, Hiroshi Tsuchikawa, Nobuaki Matsumori, Masanao Kinoshita, Md. Abdullah Al Sazzad, J. Peter Slotte, Michio Murata
Biophysical Journal 115 ( 8 ) 1530 - 1540 2018.10
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Evidence of lipid rafts based on the partition and dynamic behavior of sphingomyelins Reviewed
Masanao Kinoshita, Kenichi G.N. Suzuki, Michio Murata, Nobuaki Matsumori
Chemistry and Physics of Lipids 215 84 - 95 2018.9
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Preparation and Membrane Properties of Oxidized Ceramide Derivatives Reviewed
Langmuir 34 ( 1 ) 465 - 471 2018.1
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The impact of metal complex lipids on viscosity and curvature of hybrid liposomes Reviewed
Ryo Ohtani, Tsukasa Tokita, Tomohisa Takaya, Koichi Iwata, Masanao Kinoshita, Nobuaki Matsumori, Masaaki Nakamura, Leonard F. Lindoy, Shinya Hayami
CHEMICAL COMMUNICATIONS 53 ( 99 ) 13249 - 13252 2017.12
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Emphatic visualization of sphingomyelin-rich domains by inter-lipid FRET imaging using fluorescent sphingomyelins Reviewed
Masanao Kinoshita, Hikaru Ano, Michio Murata, Kenta Shigetomi, Junichi Ikenouchi, Nobuaki Matsumori
SCIENTIFIC REPORTS 7 ( 7 ) 16801 2017.12
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Channel Formation and Membrane Deformation via Sterol-Aided Polymorphism of Amphidinol 3 Reviewed
Masayuki Iwamoto, Ayumi Sumino, Eri Shimada, Masanao Kinoshita, Nobuaki Matsumori, Shigetoshi Oiki
SCIENTIFIC REPORTS 7 ( 7 ) 10782 2017.9
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Sterol-dependent membrane association of the marine sponge-derived bicyclic peptide Theonellamide A as examined by H-1 NMR Reviewed
Kimberly Cornelio, Rafael Atillo Espiritu, Yasuto Todokoro, Shinya Hanashima, Masanao Kinoshita, Nobuaki Matsumori, Michio Murata, Shinichi Nishimura, Hideaki Kakeya, Minoru Yoshida, Shigeki Matsunaga
BIOORGANIC & MEDICINAL CHEMISTRY 24 ( 21 ) 5235 - 5242 2016.11
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Marine sponge cyclic peptide theonellamide A disrupts lipid bilayer integrity without forming distinct membrane pores Reviewed
Rafael Atillo Espiritu, Kimberly Cornelio, Masanao Kinoshita, Nobuaki Matsumori, Michio Murata, Shinichi Nishimura, Hideaki Kakeya, Minoru Yoshida, Shigeki Matsunaga
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 1858 ( 6 ) 1373 - 1379 2016.6
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Novel Raman-tagged sphingomyelin that closely mimics original raft-forming behavior Reviewed
Jin Cui, Shigeru Matsuoka, Masanao Kinoshita, Nobuaki Matsumori, Fuminori Sato, Michio Murata, Jun Ando, Hiroyuki Yamakoshi, Kosuke Dodo, Mikiko Sodeoka
BIOORGANIC & MEDICINAL CHEMISTRY 23 ( 13 ) 2989 - 2994 2015.7
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Sphingomyelin distribution in lipid rafts of artificial monolayer membranes visualized by Raman microscopy Reviewed
Jun Ando, Masanao Kinoshita, Jin Cui, Hiroyuki Yamakoshi, Kosuke Dodo, Katsumasa Fujita, Michio Murata, Mikiko Sodeoka
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 112 ( 15 ) 4558 - 4563 2015.4
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Coexistence of two liquid crystalline phases in dihydrosphingomyelin and dioleoylphosphatidylcholine binary mixtures Reviewed
Masanao Kinoshita, Nobuaki Matsumori, Michio Murata
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 1838 ( 5 ) 1372 - 1381 2014.5
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Detailed Comparison of Deuterium Quadrupole Profiles between Sphingomyelin and Phosphatidylcholine Bilayers Reviewed
Tomokazu Yasuda, Masanao Kinoshita, Michio Murata, Nobuaki Matsumori
BIOPHYSICAL JOURNAL 106 ( 3 ) 631 - 638 2014.2
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Characterization of the ordered phase formed by sphingomyelin analogues and cholesterol binary mixtures
Masanao Kinoshita, Sarah Goretta, Hiroshi Tsuchikawa, Nobuaki Matsumori, Michio Murata
BIOPHYSICS 9 ( 0 ) 37 - 49 2013.3
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Effects of chemical modification of sphingomyelin ammonium group on formation of liquid-ordered phase Reviewed
Sarah A. Goretta, Masanao Kinoshita, Shoko Mori, Hiroshi Tsuchikawa, Nobuaki Matsumori, Michio Murata
BIOORGANIC & MEDICINAL CHEMISTRY 20 ( 13 ) 4012 - 4019 2012.7
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Kinetics for the subgel phase formation in DPPC/DOPC mixed bilayers Reviewed
Masanao Kinoshita, Keisuke Ito, Satoru Kato
CHEMISTRY AND PHYSICS OF LIPIDS 163 ( 7 ) 712 - 719 2010.9
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NaCl-dependent formation of the highly crystalline phase in sufficiently hydrated dimyristoylphosphatidylglycerol bilayers Reviewed
Masanao Kinoshita, Satoru Kato, Hiroshi Takahashi
CHEMISTRY AND PHYSICS OF LIPIDS 161 ( 1 ) 1 - 10 2009.9
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Intermolecular interaction of phosphatidylinositol with the lipid raft molecules sphingomyelin and cholesterol Reviewed
Masanao Kinoshita, Satoru Kato
Biophysics 4 1 - 9 2008.6
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Effect of bilayer morphology on the subgel phase formation
Masanao Kinoshita, Satoru Kato, Hiroshi Takahashi
Chemistry and Physics of Lipids 151 ( 1 ) 30 - 40 2008.1
Presentations
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Analysis of the lipid packing structures inside a single membrane domain by low-flux scanning electron diffraction International conference
@Masanao Kinoshita, #Shimpei Yamaguchi, @Nobuaki Matsumori
PacifiChem 2021.12
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低流量電子線散乱を用いた脂質二重層膜に形成される脂質ラフト様秩序相内部の構造解析
#前田 万優、@木下 祥尚、@松森 信明
日本化学会 第102春季年会 2022.3
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脂質の分布や挙動を高精度で再現するプローブを用いた脂質ラフトの可視化 Invited
@木下 祥尚
日本膜学会第42 年会 2020.6
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Utility of SPR analysis in elucidation of lipid function for membrane protein activation
2020.3
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ジミリストイルホスファチジルコリン（DMPC）二十層で形成される脂質様錯体リッチドメインの物理化学的特性
#渡邊光，@木下祥尚，@大谷亮，@松森信明
日本化学会 第100春季年会 2020.3
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低流量電子線散乱法を用いた局所的な脂質充填構造の解析
@木下祥尚，#山口晋平，@松森信明
日本化学会 第100春季年会 2020.3
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DHA含有リン脂質の挙動解析
#田島悠太郎，@木下祥尚，@松森信明
日本化学会 第100春季年会 2020.3
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脂質ラフト阻害剤のハイスループットスクリーニング法の開発
#NI RUIQI，@木下祥尚，@松森信明
日本化学会 第100春季年会 2020.3
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膜貫通タンパク質バクテリオロドプシンの膜ドメインへの分配特性の解析
#近江賛泰，@木下祥尚，@松森信明
日本化学会 第100春季年会 2020.3
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SPR 定量化法による脂質相互作用探索と膜タンパク質活性化メカニズムの解明
#稲田壮峰，@木下祥尚，岩本真幸，老木成稔，@松森信明
日本分析化学会 2019.5
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膜タンパク質-脂質相互作用に対する定量評価法の開発および特異的脂質の同定
#稲田壮峰, @木下祥尚，@松森信明
日本分析化学会 2019.9
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アシル鎖長の異なるスフィンゴミエリンがドメイン形成に与える影響ー蛍光スフィンゴミエリンを用いた検討
#平野佳奈，@木下祥尚， @松森信明
日本化学会 2019.3
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アミノ化セラミド誘導体の調製と膜物性分析
安田裕貴, 木下祥尚, 松森信明
膜シンポジウム 2019.11
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アシル鎖長の異なる蛍光脂質を用いたドメイン形成の解析
#平野佳奈, @木下祥尚, @松森信明
膜シンポジウム 2019.11
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Low-flux election-diffraction discloses the local structure of lipid membrane
#Shimpei Yamaguchi, @Masanao Kinoshita, @Nobuaki Matsumori
2019.9
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Identification of membrane proteins-specific lipids using surface plasmon resonance analysis: For elucidating the physiology of lipids
2019.9
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Local anesthetic molecule dibucaine disrupts raft-like/non-raft like phase separation formed in artificial membranes
@Masanao Kinoshita, #Takeshi Chitose, @Nobuaki Matsumori
2019.9
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Physicochemical properties of raft-like ordered domains formed in outer leaflet and its influence on the inner leaflet
#Takuya Koga, @Masanao Kinoshia, @Nouaki Matsumori
2019.9
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Effect of specific lipid interaction on membrane protein activities revealed by surface plasmon resonance analysis. International conference
International Conference on Bioscience of Lipids 2019.6
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脂質固定ビーズを用いた脂質特異的結合タンパク質の同定
#安田裕貴, #松藤嵩明, @木下祥尚, @松森信明
万有有明シンポジウム 2019.5
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脂質間FRETを用いたコントラストの強調とスフィンゴミエリンリッチ領域の可視化
木下祥尚，阿野光，重富健太，村田道雄，池内順一，松森信明
日本化学会 2019.3
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Detailed insight for the effect of specific interaction with lipids on membrane protein activation
2019.3
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低流量電子線散乱法を用いた局所的な脂質充填構造の解析
山口晋平，木下祥尚， 松森信明
日本化学会 2019.3
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脂質膜内葉と外葉の非対称性が人工ラフト膜の構造や物性に与える影響
古閑卓也，木下祥尚，松森信明
日本化学会 2019.3
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脂質固定ビーズによる脂質特異的タンパク質の同定
安田裕貴， 松藤嵩明，木下祥尚，松森信明
日本化学会 2019.3
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人工膜脂質に対する吸入麻酔剤の作用
川口翼，木下祥尚，松森信明
日本化学会 2019.3
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生体膜中での「脂質」の分布や挙動を高精度で評価する蛍光プローブの開発
木下祥尚
新技術説明会 2019.2
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Preparation and bilayer properties of oxidized and fluorescent derivatives of ceramide
#Takaaki Matsufuji, @Masanao Kinoshita, @J. Peter Slotte, @Nobuaki Matsumori
International seminar on biophysics and chemical biology of biomembrane and lipid bilayers 2017.10
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ジヒドロスフィンゴミエリンの蛍光標識化と蛍光顕微鏡観察
#京拓実,@木下祥尚,@松森信明
第6回CSJ化学フェスタ 2016.11
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新規セラミド蛍光誘導体の合成と蛍光顕微鏡観察
#松藤嵩明,@木下祥尚@,松森信明
CSJ化学フェスタ2016 2016.11
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脂質ラフトに対する局所麻酔剤の影響
#千歳傑, @木下祥尚, @松森信明
CSJ化学フェスタ2016 2016.11
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Inter-lipid FRET titration reveals cluster formability of the raft lipids International conference
#Takumi Kyo, @Masanao Kinoshita, @Nobuaki Matsumori
2018.1
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脂質ラフトに対する局所麻酔剤の影響
#千歳傑,@木下祥尚,@松森信明
生体膜と薬物の相互作用シンポジウム 2017.10
MISC
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Excellent Fluorescent Sphingomyelin Analog Reveals the Existence of Lipid Rafts
Masanao KINOSHITA, Nobuaki MATSUMORI
Seibutsu Butsuri 2018.11
Research Projects
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脂質ラフトに対する膜タンパク質取り込みの機序とシャペロン仮説の実証
Grant number：20K06590 2023 - 2025
日本学術振興会 科学研究費助成事業 基盤研究(C)
Authorship：Principal investigator Grant type：Scientific research funding
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脂質ラフトに対する膜タンパク質取り込みの機序とシャペロン仮説の実証
Grant number：23K05719 2023 - 2025
日本学術振興会 科学研究費助成事業 基盤研究(C)
Authorship：Principal investigator Grant type：Scientific research funding
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機能性分子としての適用を指向した生体膜に対するトリプチセン誘 導体の作用解析
2023
2023年度物質・デバイス領域共同研究拠点 共同研究課題
Authorship：Principal investigator Grant type：Contract research
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脂質ラフトをターゲトとする新たな局所麻酔薬の作用機構の提唱
Grant number：20K06590 2020 - 2022
日本学術振興会 科学研究費助成事業 基盤研究(C)
Grant type：Scientific research funding
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膜貫通タンパク質の脂質ラフトへの取り込みと機能発現の相関
2018
科学研究費助成事業 特定奨励費
Authorship：Principal investigator Grant type：Scientific research funding
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膜貫通タンパク質の脂質ラフトへの取り込みと機能発現の相間
2018
金子成田タンパク質研究奨励金
Grant type：Contract research
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電子線ナノ散乱法の開発と脂質ラフトの構造解明
Grant number：17K15107 2017 - 2019
科学研究費助成事業 若手研究(B)
Authorship：Principal investigator Grant type：Scientific research funding
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電子線ナノ散乱法の開発と脂質ラフトの構造解明
Grant number：17K15107 2017 - 2019
科学研究費助成事業 若手研究(B)
Grant type：Scientific research funding
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JST-ERATO村田脂質活性構造プロジェクト
2011.10 - 2016.10
日本
Authorship：Coinvestigator(s)
Class subject
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分析化学実験(分光光度法)
2023.10 - 2024.3 Second semester
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化学序説
2023.4 - 2024.3 Full year
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化学序説
2022.10 - 2023.3 Second semester
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分析化学実験
2022.10 - 2023.3 Second semester
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分析化学特論II
2022.4 - 2022.9 First semester
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分析化学実験
2021.10 - 2022.3 Second semester
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分析化学特論II
2021.4 - 2021.9 First semester
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分析化学実験
2020.10 - 2020.12 Fall quarter
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分析化学実験
2019.10 - 2019.12 Fall quarter
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分析化学特論II
2019.4 - 2019.9 First semester
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分析化学実験
2018.10 - 2019.3 Second semester
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化学序説
2018.4 - 2018.9 First semester
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分析化学実験
2017.10 - 2018.3 Second semester
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分析化学特論II
2017.4 - 2017.9 First semester
-
化学序説
2017.4 - 2017.9 First semester
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分析化学実験
2016.10 - 2017.3 Second semester
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化学序説
2016.4 - 2016.9 First semester
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分析化学実験
2015.10 - 2016.3 Second semester
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分析化学特論II
2015.4 - 2015.9 First semester
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化学序説
2015.4 - 2015.9 First semester