Updated on 2024/10/02

Information

 

写真a

 
OKETANI RYOSUKE
 
Organization
Faculty of Science Department of Chemistry Assistant Professor
School of Sciences Department of Chemistry(Joint Appointment)
Graduate School of Sciences Department of Chemistry(Joint Appointment)
Title
Assistant Professor
Contact information
メールアドレス
External link

Degree

  • Ph.D in Engineering

Research History

  • 大阪大学大学院工学研究科特任研究員(常勤)

Research Interests・Research Keywords

  • Research theme:Label-free microscopy for bio imaging

    Keyword:Label free, Super resolution microscopy, Optical microscope

    Research period: 2021.4

Awards

  • 日本分光学会年次講演会若手講演賞(2023年度)

    2023.10   日本分光学会   Bessel ビームを用いた multiplex CARS 顕微鏡の色収差補償

  • 2018年電子・情報・システム部門研究会奨励賞

    2018.3   電気学会 電子・情報・システム部門 光・量子デバイス研究会   2光子励起とその飽和を用いた超解像蛍光イメージング

  • 大阪大学工学研究科長表彰

    2015.3   大阪大学   第5回こども科学の教室「スーパー光塾~君も光博士になろう~」学生スタッフ代表

  • First Place Student Presentation

    2014.7   Asia Student Photonics Conference 2014  

  • 大阪大学工学研究科長表彰

    2014.3   大阪大学   第4回こども科学の教室「スーパー光塾~君も光博士になろう~」学生スタッフ代表

  • Best Student Paper Award

    2013.12   Optics & Photonics Taiwan the International Conference 2013  

  • Best Presentation Award

    2013.9  

  • Best Poster Presentation Award

    2013.7   Asia Student Photonics Conference 2013  

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Papers

  • Selective-plane-activation structured illumination microscopy Reviewed International coauthorship

    Kenta Temma, Ryosuke Oketani, Toshiki Kubo, Kazuki Bando, Shunsuke Maeda, Kazunori Sugiura, Tomoki Matsuda, Rainer Heintzmann, Tatsuya Kaminishi, Koki Fukuda, Maho Hamasaki, Takeharu Nagai, Katsumasa Fujita

    Nature Methods   2024.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41592-024-02236-3

  • Molecular Fingerprinting of Mouse Brain Using Ultrabroadband Coherent Anti-Stokes Raman Scattering (CARS) Microspectroscopy Empowered by Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) Invited Reviewed International coauthorship International journal

    Murakami Y., Ando M., Imamura A., Oketani R., Leproux P., Honjoh S., Kano H.

    Chemical and Biomedical Imaging   2024

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Chemical and Biomedical Imaging  

    The Raman fingerprint spectral region provides abundant structural information on molecules. However, analyzing vibrational images within this region using coherent Raman imaging remains challenging due to the small Raman cross section and congested spectral features. In this study, we combined ultrabroadband coherent anti-Stokes Raman scattering (CARS) microspectroscopy across the spectral range of 500-4000 cm-1 with multivariate curve resolution-alternating least-squares (MCR-ALS) to reveal hidden Raman bands in the fingerprint region. Applying this method to mouse brain tissue, we extracted information on cholesterol and collagen, leveraging their distinctive molecular signatures, as well as on key molecules such as lipids, proteins, water, and nucleic acids. Moreover, the simultaneous detection of second harmonic generation facilitated label-free visualization of organelles, including arachnoid membrane and Rootletin filaments.

    DOI: 10.1021/cbmi.4c00034

    Scopus

  • Saturated-excitation image scanning microscopy for super-resolution fluorescence imaging

    Biomedical Imaging and Sensing Conference   2023.9

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    Language:Others   Publishing type:Research paper (other academic)  

    DOI: 10.1117/12.3007909

  • Backward multiplex coherent anti-Stokes Raman (CARS) spectroscopic imaging with electron-multiplying CCD (EMCCD) camera Reviewed

    YUSUKE MURAKAMI, Minami Yoshimura, W.J. Klement, Atsuki Oda, Ryo Sakamoto, Miho Yakabe, Atsushi Matsumoto, Ryosuke Oketani, Philippe Leproux, Junichi Ikenouchi, Wesley Browne, Hideaki Kano

    Optics Continuum   2 ( 9 )   2044 - 2054   2023.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1364/optcon.497869

  • Selective plane activation structured illumination microscopy

    Kenta Temma, Ryosuke Oketani, Toshiki Kubo, Kazuki Bando, Shunsuke Maeda, Kazunori Sugiura, Tomoki Matsuda, Rainer Heintzmann, Tatsuya Kaminishi, Koki Fukuda, Maho Hamasaki, Takeharu Nagai, Katsumasa Fujita

    bioRxiv   2023.6

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    Language:Others  

    DOI: 10.1101/2023.06.19.545558

  • Label-Free Identification of Spore-Forming Bacteria Using Ultrabroadband Multiplex Coherent Anti-Stokes Raman Scattering Microspectroscopy Reviewed

    Kyosuke Tanaka, Ryosuke Oketani, Takeshi Terada, Philippe Leproux, Yuki Morono, Hideaki Kano

    The Journal of Physical Chemistry B   127 ( 9 )   1940 - 1946   2023.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acs.jpcb.2c07291

  • Bessel-beam illumination Raman microscopy Reviewed

    Kazuki Bando, Shumpei Yabuuchi, Menglu Li, Toshiki Kubo, Ryosuke Oketani, Nicholas I. Smith, Katsumasa Fujita

    Biomedical Optics Express   13 ( 6 )   3161 - 3170   2022.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1364/BOE.456138

  • Saturated-excitation image scanning microscopy Reviewed

    Optics Express   30 ( 8 )   13825 - 13838   2022.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1364/oe.455621

  • Spectroscopic second and third harmonic generation microscopy using a femtosecond laser source in the third near-infrared (NIR-III) optical window Reviewed

    Yusuke Murakami, Minori Masaki, Shinichi Miyazaki, Ryosuke Oketani, Yu Hayashi, Masashi Yanagisawa, Sakiko Honjoh, Hideaki Kano

    Biomedical Optics Express   13 ( 2 )   694 - 708   2022.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1364/boe.446273

  • Using saturated absorption for super‐resolution laser scanning transmission microscopy Reviewed

    288 ( 2 )   117 - 129   2021.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/jmi.13033

  • Multiphoton-Excited Deep-Ultraviolet Photolithography for 3D Nanofabrication Reviewed

    Atsushi Taguchi, Atsushi Nakayama, Ryosuke Oketani, Satoshi Kawata, Katsumasa Fujita

    ACS Applied Nano Materials   3 ( 11 )   11434 - 11441   2020.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acsanm.0c02519

  • Visible-wavelength two-photon excitation microscopy with multifocus scanning for volumetric live-cell imaging Reviewed

    Ryosuke Oketani, Haruka Suda, Kumiko Uegaki, Toshiki Kubo, Tomoki Matsuda, Masahito Yamanaka, Yoshiyuki Arai, Nicholas I. Smith, Takeharu Nagai, Katsumasa Fujita

    Journal of Biomedical Optics   25 ( 01 )   014502 - 5   2019.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1117/1.jbo.25.1.014502

  • Saturated excitation microscopy using differential excitation for efficient detection of nonlinear fluorescence signals Reviewed

    Yasunori Nawa, Yasuo Yonemaru, Atsushi Kasai, Ryosuke Oketani, Hitoshi Hashimoto, Nicholas I. Smith, Katsumasa Fujita

    APL Photonics   3 ( 8 )   080805 - 080805   2018.8

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    Language:Others   Publishing type:Research paper (scientific journal)  

    DOI: 10.1063/1.5039567

  • High-resolution imaging in two-photon excitation microscopy using in situ estimations of the point spread function Reviewed

    Atsushi Doi, Ryosuke Oketani, Yasunori Nawa, Katsumasa Fujita

    Biomedical Optics Express   9 ( 1 )   202 - 213   2018.1

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    Language:Others   Publishing type:Research paper (scientific journal)  

    DOI: 10.1364/boe.9.000202

  • Saturated two-photon excitation fluorescence microscopy with core-ring illumination Reviewed

    Ryosuke Oketani, Atsushi Doi, Nicholas I. Smith, Yasunori Nawa, Satoshi Kawata, Katsumasa Fujita

    Optics Letters   42 ( 3 )   571 - 574   2017.2

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    Language:Others   Publishing type:Research paper (scientific journal)  

    DOI: 10.1364/ol.42.000571

  • Study of Nonlinear Plasmonic Scattering in Metallic Nanoparticles Reviewed

    Yu-Ting Chen, Po-Hsuan Lee, Po-Ting Shen, Jann Launer, Ryosuke Oketani, Kuan-Yu Li, Yen-Ta Huang, Kyoko Masui, Satoru Shoji, Katsumasa Fujita, Shi-Wei Chu

    ACS Photonics   3 ( 8 )   1432 - 1439   2016.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acsphotonics.6b00025

  • Ultrasmall all-optical plasmonic switch and its application to superresolution imaging Reviewed

    Hsueh-Yu Wu, Yen-Ta Huang, Po-Ting Shen, Hsuan Lee, Ryosuke Oketani, Yasuo Yonemaru, Masahito Yamanaka, Satoru Shoji, Kung-Hsuan Lin, Chih-Wei Chang, Satoshi Kawata, Katsumasa Fujita, Shi-Wei Chu

    Scientific Reports   6 ( 1 )   24293   2016.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/srep24293

  • Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle Reviewed

    Hsuan Lee, Kuan-Yu Li, Yen-Ta Huang, Po-Ting Shen, Gitanjal Deka, Ryosuke Oketani, Yasuo Yonemaru, Masahito Yamanaka, Katsumasa Fujita, Shi-Wei Chu

    Journal of Visualized Experiments   ( 107 )   e53338   2016.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3791/53338

  • Point spread function analysis with saturable and reverse saturable scattering Reviewed

    Hsuan Lee, Ryosuke Oketani, Yen-Ta Huang, Kuan-Yu Li, Yasuo Yonemaru, Masahito Yamanaka, Satoshi Kawata, Katsumasa Fujita, Shi-Wei Chu

    Optics Express   22 ( 21 )   26016 - 26022   2014.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1364/oe.22.026016

  • Measurement of a Saturated Emission of Optical Radiation from Gold Nanoparticles: Application to an Ultrahigh Resolution Microscope Reviewed

    Shi-Wei Chu, Tung-Yu Su, Ryosuke Oketani, Yen-Ta Huang, Hsueh-Yu Wu, Yasuo Yonemaru, Masahito Yamanaka, Hsuan Lee, Guan-Yu Zhuo, Ming-Ying Lee, Satoshi Kawata, Katsumasa Fujita

    Physical Review Letters   112 ( 1 )   017402   2014.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1103/physrevlett.112.017402

  • Saturation and Reverse Saturation of Scattering in a Single Plasmonic Nanoparticle Reviewed

    Shi-Wei Chu, Hsueh-Yu Wu, Yen-Ta Huang, Tung-Yu Su, Hsuan Lee, Yasuo Yonemaru, Masahito Yamanaka, Ryosuke Oketani, Satoshi Kawata, Satoru Shoji, Katsumasa Fujita

    ACS Photonics   1 ( 1 )   32 - 37   2014.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/ph4000218

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Presentations

  • Bessel ビームを用いた multiplex CARS 顕微鏡の色収差補償

    桶谷 亮介, 田中 京介, 加納 英明

    2023年度日本分光学会年次講演会  2023.10 

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    Event date: 2023.10

    Language:Japanese  

    Country:Other  

  • 超解像イメージング:飽和したプラズモン光散乱の利用

    桶谷 亮介, Tung-Yu Su, 米丸 泰央, Hsuan Lee, 山中 真仁, Ming-Ying Lee, 河田 聡, Shi-Wei Chu, 藤田 克昌

    第60回応用物理学会春季学術講演会  2013.3 

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    Language:Japanese  

    Country:Other  

  • Development of super-resolution microscopy by means of saturable plasmon scattering of metal nano particles

    Ryosuke Oketani, Tung-Yu Su, Yasuo Yonemaru, Hsuan Lee, Masahito Yamanaka, Ming-Ying Lee, Satoshi Kawata, Shi-Wei Chu, Katsumasa Fujita

    NCS-JST Workshop on Nano Devices  2013.3 

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    Language:English  

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  • Super-resolution Imaging of Gold Nanoparticles Based on Saturation of Plasmonic Light Scattering

    Ryosuke Oketani, Tung-Yu Su, Yasuo Yonemaru, Hsuan Lee, Masahito Yamanaka, Ming-Ying Lee, Satoshi Kawata, Shi-Wei Chu, Katsumasa Fujita

    2013 Conference on Lasers and Electro-Optics Pacific Rim  2013.7 

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  • Super-Resolution Microscopy by Using Saturation of Plasmonic Light Scattering

    Ryosuke Oketani, Tung-Yu Su, Yasuo Yonemaru, Hsuan Lee, Masahito Yamanaka, Ming-Ying Lee, Satoshi Kawata, Shi-Wei Chu, Katsumasa Fujita

    Asia Student Photonics Conference 2013  2013.7 

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    Language:English  

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  • Spatial Resolution Boosting for Confocal Microscopy by Using Saturation of Plasmonic Light Scattering

    Ryosuke Oketani, Tung-Yu Su, Masahito Yamanaka, YasuoYonemaru, Yen-Ta Huang, Hsueh-Yu Wu, Hsuan Lee, Guan-Yu Zhuo, Ming-Ying Lee, Satoshi Kawata, Shi-Wei Chu, Katsumasa Fujita

    Optics & Photonics Taiwan the International Conference 2013  2013.12 

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  • 金ナノ粒子の散乱光飽和を用いた超解像イメージング

    応用物理学会関西支部 平成25年度第3回講演会「関西からのフォトニクスとカーボン研究イノベーション」  2014.2 

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    Language:Japanese  

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  • Nonlinear Scattering from Metallic Nanoparticles And its Application to Super Resolution Imaging

    Ryosuke Oketani, Tung-Yu-Su, Hsueh-Yu Wu, Hsuan Lee, Yen-Ta Huang, Yasuo Yonemaru, Satoshi Kawata, Shi-Wei Chu, Katsumasa Fujita

    Asia Student Photonics Conference 2014  2014.7 

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    Language:English  

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  • Super-Resolution Microscopy by Using Saturation of Plasmonic Light Scattering

    Ryosuke Oketani, Tung-Yu Su, Yasuo Yonemaru, Hsuan Lee, Masahito Yamanaka, Ming-Ying Lee, Satoshi Kawata, Shi-Wei Chu, Katsumasa Fujita

    2014.9 

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  • Nonlinear Scattering from Gold Nanoparticles And its Application to High Resolution Imaging

    Ryosuke Oketani, Tung-Yu-Su, Hsueh-Yu Wu, Hsuan Lee, Yen-Ta Huang, Yasuo Yonemaru, Satoshi Kawata, Shi-Wei Chu, Katsumasa Fujita

    International OSA Network of Students 5 Hokkaido  2014.9 

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  • Modification of Point-Spread Function in Confocal Microscopy by Nonlinear Plasmonic Light Scattering

    Ryosuke Oketani, Hsueh-Yu Wu, Hsuan Lee, Yen-Ta Huang, Yasuo Yonemaru, Tung-Yu Su, Satoshi Kawata, Shi-Wei Chu, Katsumasa Fujita

    JSAP-OSA Joint Symposia 2014  2014.9 

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  • Point-Spread Function of Confocal Microscopy Modified by Nonlinear Plasmonic Light Scattering

    Ryosuke Oketani, Hsueh-Yu Wu, Hsuan Lee, Yen-Ta Huang, Yasuo Yonemaru, Tung-Yu Su, Satoshi Kawata, Shi-Wei Chu, Katsumasa Fujita

    Japan-Singapore Workshop on Nanophotonics, Plasmonics, and Metamaterials  2014.12 

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  • Modification of Point-Spread Function in Confocal Imaging by Using Nonlinear Light Scattering

    Ryosuke Oketani, Hsueh-Yu Wu, Hsuan Lee, Yen-Ta Huang, Yasuo Yonemaru, Tung-Yu Su, Satoshi Kawata, Shi-Wei Chu, Katsumasa Fujita

    Optics & Photonics Taiwan the International Conference 2014  2014.12 

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  • High-Resolution Fluorescence Imaging by Saturated Two-Photon Excitation with Core-Ring Illumination

    Ryosuke Oketani, Atsushi Doi, Nicholas I. Smith, Yasunori Nawa, Satoshi Kawata, Katsumasa Fujita

    The 24th General Congress of the International Commission for Optics  2017.8 

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  • High-Resolution Fluorescence Imaging by Saturated Two-Photon Excitation with Core-Ring Illumination

    Ryosuke Oketani, Atsushi Doi, Nicholas I. Smith, Yasunori Nawa, Satoshi Kawata, Katsumasa Fujita

    INP-2017, The international Nanophotonics Symposium  2017.8 

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  • Super-Resolution in Two-Photon Excitation Microscopy via Saturated Excitation

    Ryosuke Oketani, Atsushi Doi, Nicholas I. Smith, Yasunori Nawa, Satoshi Kawata, Katsumasa Fujita

    JSAP-OSA Joint Symposia 2017  2017.9 

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  • 2光子励起の飽和を利用した超解像イメージング

    光子励起の飽和を利用した超解像イメージング, 桶谷亮介, 土井厚志, ミヤサキ ケートリン, 久保俊貴, 名和靖矩, 河田 聡, 藤田克昌

    2017.11 

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    Language:Japanese  

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  • 2光子励起の飽和とCore-ring照明を利用した 超解像イメージング

    桶谷亮介, 土井厚志, Nicholas I. Smith, 名和靖矩, 河田聡, 藤田克昌

    第43回レーザ顕微鏡研究会&シンポジウム  2018.1 

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  • 2 光子励起とその飽和を用いた超解像蛍光イメージング

    桶谷亮介, 土井厚志, ミヤサキケートリン, 久保俊貴, 名和靖矩, 河田聡, 藤田克昌

    電気学会 電子・情報・システム部門 光・量子デバイス研究会  2018.3 

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    Language:Japanese  

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  • 可視光を用いた 2 光子励起顕微鏡による 3 次元生細胞イメージング

    桶谷亮介, 上垣久美子, 久保俊貴, 松田知己, スミスニコラス, 永井健治, 藤田克昌

    2018.11 

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  • 2 光子励起とその飽和を用いた超解像蛍光イメージング Invited

    桶谷亮介

    平成 30 年 電子・情報・システム部門 優秀論文発表賞 A 賞,研究会奨励賞,技術委員会奨励賞 受賞記念講演会  2019.3 

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  • 可視光を用いた2光子励起多点走査顕微鏡による 3 次元生細胞イメージング

    桶谷亮介, 須田悠, 上垣久美子, 久保俊貴, 松田知己, 山中真仁, 新井由之, Nicholas I, Smith, 永井健治, 藤田克昌

    レーザ顕微鏡研究会 第44回講演会  2019.7 

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  • Not so long time ago in my student life, filed away…. Invited

    2019.11 

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  • スピニングディスク共焦点蛍光顕微鏡の線型および可視域二光子励起における結像特性

    須田悠, 桶谷亮介, 藤田克昌

    2019.12 

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  • Besselビーム照明による色収差補正を用いたmultiplex非線形ラマンイメージング

    桶谷 亮介, 田中 京介, 加納 英明

    第84回応用物理学会秋季学術講演会  2023.9 

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MISC

  • 応用物理学会学生チャプターは,暗い部屋に集まってコソコソ活動しているわけじゃない!

    桶谷亮介

    応用物理   2015.2

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    Language:Japanese  

Professional Memberships

  • The Japan Society of Applied Physics

  • Optica

  • the Spectroscopical Society of Japan

Research Projects

  • 脳波のコヒーレンスを生み出す細胞間協同作用のin-vivoラベルフリー可視化

    Grant number:21H04961  2021 - 2023

    日本学術振興会  科学研究費助成事業  基盤研究(A)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • コヒーレントラマン分光と信号飽和を利用した観察対象を指定しない超解像顕微鏡の開発

    Grant number:21K20505  2021 - 2022

    日本学術振興会  科学研究費助成事業  研究活動スタート支援

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 金属ナノ粒子におけるプラズモン光散乱の非線形特性解明とその光学機器への応用

    Grant number:15J01780  2015 - 2016

    日本学術振興会  科学研究費助成事業  特別研究員奨励費

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    Authorship:Principal investigator  Grant type:Scientific research funding

Class subject

  • 物理化学実験

    2023.12 - 2024.2   Winter quarter

  • 物理化学実験

    2022.12 - 2023.2   Winter quarter

  • 物理化学実験

    2021.12 - 2022.2   Winter quarter

  • 化学序説(R3年度入学者及び2年次未進級者用)

    2021.4 - 2022.3   Full year

  • 化学序説(R2年度以前入学者用)

    2021.4 - 2021.9   First semester

FD Participation

  • 2022.7   Role:Participation   Title:【化学科FD】電子ジャーナルに関するFD

    Organizer:Undergraduate school department

  • 2021.4   Role:Participation   Title:2021年度新任教員教育セミナー

    Organizer:University-wide

  • 2021.4   Role:Participation   Title:2021年度・第1回全学FD(新任教員の研修)

    Organizer:University-wide