Updated on 2026/05/18

Information

 

写真a

 
OKETANI RYOSUKE
 
Organization
Faculty of Science Department of Chemistry Assistant Professor
School of Sciences Department of Chemistry（Concurrent）
Graduate School of Sciences Department of Chemistry（Concurrent）
Title
Assistant Professor
Contact information
メールアドレス
Homepage
External link

Research Areas

  • Life Science / Biomedical engineering

  • Nanotechnology/Materials / Optical engineering and photon science

  • Nanotechnology/Materials / Optical engineering and photon science

Degree

  • Ph.D in Engineering

Research History

  • Kyushu University Faculty of Sciences Assistant Professor 

    2021.4 - Present

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  • 大阪大学大学院工学研究科特任研究員（常勤）   

    2017.4 - 2021.3

Education

  • Osaka University   Graduate school of engineering   Department of applied phisics

    2014.4 - 2017.3

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    Country：Japan

Research Interests・Research Keywords

  • Research theme： Super resolution Microscopy

    Keyword： Super resolution Microscopy

    Research period： 2026

  • Research theme： Fluorescence microscopy

    Keyword： Fluorescence microscopy

    Research period： 2026

  • Research theme： Optical microscope

    Keyword： Optical microscope

    Research period： 2026

  • Research theme： Label-free microscopy

    Keyword： Label-free microscopy

    Research period： 2026

  • Research theme： Label-free microscopy for bio imaging

    Keyword： Label free, Super resolution microscopy, Optical microscope

    Research period： 2021.4

Awards

  • 日本分光学会年次講演会若手講演賞（2023年度）

    2023.10   日本分光学会   Bessel ビームを用いた multiplex CARS 顕微鏡の色収差補償

    桶谷亮介

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  • 2018年電子・情報・システム部門研究会奨励賞

    2018.3   電気学会 電子・情報・システム部門 光・量子デバイス研究会   2光子励起とその飽和を用いた超解像蛍光イメージング

  • 大阪大学工学研究科長表彰

    2015.3   大阪大学   第５回こども科学の教室「スーパー光塾～君も光博士になろう～」学生スタッフ代表

  • First Place Student Presentation

    2014.7   Asia Student Photonics Conference 2014   Nonlinear Scattering from Metallic Nanoparticles And its Application to Super Resolution Imaging

  • 大阪大学工学研究科長表彰

    2014.3   大阪大学   第４回こども科学の教室「スーパー光塾～君も光博士になろう～」学生スタッフ代表

  • Best Student Paper Award

    2013.12   Optics & Photonics Taiwan the International Conference 2013   Spatial Resolution Boosting for Confocal Microscopy by Using Saturation of Plasmonic Light Scattering

  • Best Presentation Award

    2013.9   6th Vietnamese-Japanese Students’ Scientific Exchange Meeting   Super-Resolution Microscopy by Using Saturation of Plasmonic Light Scattering

  • Best Poster Presentation Award

    2013.7   Asia Student Photonics Conference 2013   Super-resolution Microscopy by Using Saturation of Plasmonic Light Scattering

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Papers

  • Selective-plane-activation structured illumination microscopy Reviewed International coauthorship

    Kenta Temma, Ryosuke Oketani, Toshiki Kubo, Kazuki Bando, Shunsuke Maeda, Kazunori Sugiura, Tomoki Matsuda, Rainer Heintzmann, Tatsuya Kaminishi, Koki Fukuda, Maho Hamasaki, Takeharu Nagai, Katsumasa Fujita

    Nature Methods   2024.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    革新的３次元超解像顕微鏡の開発。

    DOI： 10.1038/s41592-024-02236-3

  • All-optical classification of real biomedical cell images using a diffractive neural network: a simulation study Reviewed

    Norihide Sagami, Yueyun Weng, Cheng Lei, Ryosuke Oketani, Kotaro Hiramatsu

    Optics Letters   51 ( 7 )   1951 - 1951   2026.3   （ ISSN:0146-9592 eISSN:1539-4794 ）

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Optica Publishing Group  

    We report an in-silico demonstration of an all-optical cell classification system using a single-layer diffractive neural network (DNN) optimized for real-world biomedical images. Implemented virtually with a spatial light modulator (SLM), the DNN was numerically trained via backpropagation to differentiate breast cells, lung cancer cells, and white blood cells. The training utilized experimentally acquired phase and amplitude images from optofluidic time-stretch quantitative phase imaging. Classification was simulated by computing the optical intensities at the detection plane. The optimized DNN achieved 96.1% accuracy, approaching that of conventional convolutional neural networks. This study highlights the potential of SLM-based DNNs for ultrafast, energy-efficient biomedical image processing in practical optical computing scenarios.

    DOI： 10.1364/ol.593231

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    Other Link： https://opg.optica.org/viewmedia.cfm?URI=ol-51-7-1951&seq=0

  • Two-Photon-Excited Photoinduced Force Microscopy Reviewed

    Tetsu Tamura, Norihide Sagami, Takeru Sasaki, Wataru Yamamoto, Ryosuke Oketani, Kotaro Hiramatsu

    Nano Letters   26 ( 1 )   51 - 57   2025.12   （ ISSN:1530-6984 eISSN:1530-6992 ）

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

    Sensing photoexcitation-induced forces via atomic force microscopy (AFM) has attracted much attention as a promising nanoscale molecular imaging modality. However, most such nanospectroscopies suffer from linear background and noise under single-photon excitation, including scattering into a quadrant detector or photothermal response from the cantilever or substrate. Here, we present two-photon-excited photoinduced force microscopy (TP-PiFM) to suppress these unwanted linear effects. A theoretical analysis shows that signal demodulation at ω<inf>tip</inf> ± 2ω<inf>opt</inf> can efficiently isolate the photothermal signal under intensity modulation on the pulse train at ω<inf>opt</inf> and tip driving at ω<inf>tip</inf>. As a proof-of-concept demonstration, we obtained TP-PiFM images of 40 nm gold nanoparticles with a higher resolution than that of AFM topography. Backgrounds in TP-PiFM images were suppressed through the demodulation technique as well as a fitting analysis to extract signals quadratically scaling on the illumination power. These results establish TP-PiFM as a robust, background-free nanoimaging method.

    DOI： 10.1021/acs.nanolett.5c04444

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  • β-Sheet alignment in intact feathers revealed by polarization-resolved second harmonic generation (SHG) and coherent Raman imaging Reviewed

    Zuliang Hu, Ryosuke Oketani, Kotaro Hiramatsu, Philippe Leproux, Ryo Suzuki, Hideaki Kano

    Chemistry Letters   54 ( 11 )   2025.11   （ ISSN:0366-7022 eISSN:1348-0715 ）

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    Abstract

    We visualized β-sheet alignment in intact feathers using polarization-resolved second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) imaging. SHG signal intensity was strongly dependent on the polarization of the incident laser, while the amide I and III bands in the CARS spectra exhibited moderate polarization-dependent behavior. These results demonstrate that SHG and CARS enable complementary, label-free assessment of structural anisotropy in β-sheet–rich protein fibers, with SHG being especially sensitive to the orientation of the peptide backbone.

    DOI： 10.1093/chemle/upaf207

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    Other Link： https://academic.oup.com/chemlett/article-pdf/54/11/upaf207/65272443/upaf207.pdf

  • Chromatic aberration compensation in multiplex coherent Raman microscopy using a Bessel beam Reviewed

    Ryosuke Oketani, Kyosuke Tanaka, Yusuke Murakami, Hideaki Kano

    Optics Letters   50 ( 19 )   6221 - 6221   2025.9   （ ISSN:0146-9592 eISSN:1539-4794 ）

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Optica Publishing Group  

    Multiplex coherent anti-Stokes Raman scattering (CARS) microscopy enables label-free spectroscopic imaging over an ultra-broadband range using a supercontinuum light source. However, under high numerical aperture conditions, chromatic aberration causes axial splitting of the illumination spot, leading to missing or significantly weakened peaks. Here, we used Bessel beam illumination to compensate for chromatic aberration without modifying objective lenses. Our numerical and experimental results demonstrate that combining the nonlinear nature of the CARS process with the axial elongation of the Bessel beam spot simultaneously achieves broadband spectroscopy and high spatial resolution imaging. This approach is expected to further expand the applicability of vibrational spectroscopic imaging technique.

    DOI： 10.1364/ol.574997

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  • Label-Free Visualization of Ciliary Rootlets in Mouse Brain Reviewed International coauthorship

    Yusuke Murakami, Mutsuo Nuriya, Zuliang Hu, Masaki Tomioka, Ryosuke Oketani, Kotaro Hiramatsu, Philippe Leproux, Akihito Inoko, Sakiko Honjoh, Hideaki Kano

    Analytical Chemistry   97 ( 27 )   14160 - 14167   2025.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/acs.analchem.4c05851

  • Exploring liquid-liquid phase separation in vitro and in vivo using multimodal nonlinear optical imaging Reviewed

    Murakami, Y; Obuchi, M; Kamizawa, H; Miyazaki, S; Kishimura, A; Oketani, R; Hiramatsu, K; Leproux, P; Hayashi, Y; Shiraki, K; Kano, H

    ANALYTICAL SCIENCES   2025.3   （ ISSN:0910-6340 eISSN:1348-2246 ）

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    Language：English   Publisher：Analytical Sciences  

    Liquid–liquid phase separation leads to the formation of liquid droplets (LqDs) such as P granules in Caenorhabditis elegans (C. elegans). In this study, we demonstrate the label-free visualization of LqDs using multimodal nonlinear optical imaging both in vitro and in vivo. In vitro measurements with polymerized adenine [poly(A)], we found significantly higher poly(A) concentrations in LqDs compared to surrounding solutions, with the limit of detection (LoD) of 32 mg/mL. In vivo measurements, we performed label-free imaging of C. elegans. Despite efforts to detect P granules within P lineage cells in both wild-type C. elegans and green fluorescent protein (GFP)-tagged strains, no clear RNA-specific signals were observed. This indicates that the RNA concentration in P granules is lower than anticipated and falls below our in vitro LoD. These results underscore the challenges of label-free RNA detection in P granules.

    DOI： 10.1007/s44211-025-00747-3

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  • Molecular Fingerprinting of Mouse Brain Using Ultrabroadband Coherent Anti-Stokes Raman Scattering (CARS) Microspectroscopy Empowered by Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) Invited Reviewed International coauthorship International journal

    Yusuke Murakami, Masahiro Ando, Ayako Imamura, Ryosuke Oketani, Philippe Leproux, Sakiko Honjoh, Hideaki Kano

    Chemical & Biomedical Imaging   2 ( 10 )   689 - 697   2024   （ ISSN:2832-3637 eISSN:2832-3637 ）

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

    The Raman fingerprint spectral region provides abundant structural information on molecules. However, analyzing vibrational images within this region using coherent Raman imaging remains challenging due to the small Raman cross section and congested spectral features. In this study, we combined ultrabroadband coherent anti-Stokes Raman scattering (CARS) microspectroscopy across the spectral range of 500-4000 cm-1 with multivariate curve resolution-alternating least-squares (MCR-ALS) to reveal hidden Raman bands in the fingerprint region. Applying this method to mouse brain tissue, we extracted information on cholesterol and collagen, leveraging their distinctive molecular signatures, as well as on key molecules such as lipids, proteins, water, and nucleic acids. Moreover, the simultaneous detection of second harmonic generation facilitated label-free visualization of organelles, including arachnoid membrane and Rootletin filaments.

    DOI： 10.1021/cbmi.4c00034

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  • Backward multiplex coherent anti-Stokes Raman (CARS) spectroscopic imaging with electron-multiplying CCD (EMCCD) camera Reviewed

    YUSUKE MURAKAMI, Minami Yoshimura, W.J. Klement, Atsuki Oda, Ryo Sakamoto, Miho Yakabe, Atsushi Matsumoto, Ryosuke Oketani, Philippe Leproux, Junichi Ikenouchi, Wesley Browne, Hideaki Kano

    Optics Continuum   2 ( 9 )   2044 - 2054   2023.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1364/optcon.497869

  • Label-Free Identification of Spore-Forming Bacteria Using Ultrabroadband Multiplex Coherent Anti-Stokes Raman Scattering Microspectroscopy Reviewed

    Kyosuke Tanaka, Ryosuke Oketani, Takeshi Terada, Philippe Leproux, Yuki Morono, Hideaki Kano

    The Journal of Physical Chemistry B   127 ( 9 )   1940 - 1946   2023.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/acs.jpcb.2c07291

  • Bessel-beam illumination Raman microscopy Reviewed

    Kazuki Bando, Shumpei Yabuuchi, Menglu Li, Toshiki Kubo, Ryosuke Oketani, Nicholas I. Smith, Katsumasa Fujita

    Biomedical Optics Express   13 ( 6 )   3161 - 3170   2022.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    We demonstrate the use of Bessel beams for side illumination slit-scanning Raman imaging for label-free and hyperspectral analysis of cell spheroids. The background elimination by the side illumination and the aberration-resistant Bessel beam drastically improves the image contrast in Raman observation, allowing label-free investigation of intracellular molecules in thick biological samples. Live cell spheroids were observed to confirm the improvement in image contrast and background reduction with Bessel illumination compared to conventional epi-line illumination.

    DOI： 10.1364/BOE.456138

  • Saturated-excitation image scanning microscopy Reviewed

    Kenta Temma, Ryosuke Oketani, René Lachmann, Toshiki Kubo, Nicholas I. Smith, Rainer Heintzmann, Katsumasa Fujita

    Optics Express   30 ( 8 )   13825 - 13838   2022.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1364/oe.455621

  • Spectroscopic second and third harmonic generation microscopy using a femtosecond laser source in the third near-infrared (NIR-III) optical window Reviewed

    Yusuke Murakami, Minori Masaki, Shinichi Miyazaki, Ryosuke Oketani, Yu Hayashi, Masashi Yanagisawa, Sakiko Honjoh, Hideaki Kano

    Biomedical Optics Express   13 ( 2 )   694 - 708   2022.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    In this study, second harmonic generation (SHG) and third harmonic generation (THG) spectroscopic imaging were performed on biological samples using a femtosecond laser source in the third near-infrared (NIR) optical window (NIR-III). Using a visible-NIR spectrometer, the SHG and THG signals were simultaneously detected and were extracted using spectral analysis. Visualization of biological samples such as cultured cells (HEK293 T), mouse brain slices, and the nematode Caenorhabditis elegans was performed in a label-free manner. In particular, in an SHG image of an entire coronal brain section (8 × 6 mm2), we observed mesh-like and filamentous structures in the arachnoid mater and wall of the cerebral ventricle, probably corresponding to the collagen fibers, cilia, and rootlet. Moreover, the THG images clearly depicted the densely packed axons in the white matter and cell nuclei at the cortex of the mouse brain slice sample and lipid-rich granules such as lipid droplets inside the nematode. The observations and conclusions drawn from this technique confirm that it can be utilized for various biological applications, including in vivo label-free imaging of living animals.

    DOI： 10.1364/boe.446273

  • Using saturated absorption for super‐resolution laser scanning transmission microscopy Reviewed

    Kentaro Nishida, Hikaru Sato, Ryosuke Oketani, Kentaro Mochizuki, Kenta Temma, Yasuaki Kumamoto, Hideo Tanaka, Katsumasa Fujita

    Journal of Microscopy   288 ( 2 )   117 - 129   2021.5

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    DOI： 10.1111/jmi.13033

  • Multiphoton-Excited Deep-Ultraviolet Photolithography for 3D Nanofabrication Reviewed

    Atsushi Taguchi, Atsushi Nakayama, Ryosuke Oketani, Satoshi Kawata, Katsumasa Fujita

    ACS Applied Nano Materials   3 ( 11 )   11434 - 11441   2020.11

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    © 2020 American Chemical Society. Light-matter interactions in the deep-ultraviolet (DUV) wavelength region exhibit a variety of optical effects such as luminescence, photoisomerization, and polymerization in many materials. Despite the rich photochemistry and high spatial resolution due to the short wavelength, the notorious lack of DUV-compatible optical components and devices precludes the use of DUV light in microscopy and lithography as a routine laboratory tool. Here, we present the use of two-photon excitation with visible laser light to realize photopolymerization of molecules with an excitation energy equivalent to DUV light. Using standard optics for visible light, we polymerized methacrylate oligomers with 400 nm femtosecond pulses without any addition of photoinitiators and sensitizers. By scanning the laser focus in 3D, we created a series of fine 3D structures with the smallest resolved line-space features of 80 nm. We found that photopolymerizations induced by two-photon absorption at DUV is surprisingly efficient and requires laser intensity only on the order of 100 kW/cm2. The use of DUV absorption sites natively existing in monomers simplifies polymerization reactions without sacrificing material purities by adding initiators and facilitates applications to diverse materials. We have applied two-photon DUV polymerizations to metal-oxo clusters and the amino acid cysteine. With the variety of successful demonstrations including organic- and inorganic-material-made-structures presented, multiphoton DUV polymerization offers a distinct tool for fabrications of nanodevices in 3D.

    DOI： 10.1021/acsanm.0c02519

  • Visible-wavelength two-photon excitation microscopy with multifocus scanning for volumetric live-cell imaging Reviewed

    Ryosuke Oketani, Haruka Suda, Kumiko Uegaki, Toshiki Kubo, Tomoki Matsuda, Masahito Yamanaka, Yoshiyuki Arai, Nicholas I. Smith, Takeharu Nagai, Katsumasa Fujita

    Journal of Biomedical Optics   25 ( 01 )   014502 - 5   2019.11

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    Two-photon excitation microscopy is one of the key techniques used to observe three-dimensional (3-D) structures in biological samples. We utilized a visible-wavelength laser beam for two-photon excitation in a multifocus confocal scanning system to improve the spatial resolution and image contrast in 3-D live-cell imaging. Experimental and numerical analyses revealed that the axial resolution has improved for a wide range of pinhole sizes used for confocal detection. We observed the 3-D movements of the Golgi bodies in living HeLa cells with an imaging speed of 2 s per volume. We also confirmed that the time-lapse observation up to 8 min did not cause significant cell damage in two-photon excitation experiments using wavelengths in the visible light range. These results demonstrate that multifocus, two-photon excitation microscopy with the use of a visible wavelength can constitute a simple technique for 3-D visualization of living cells with high spatial resolution and image contrast.

    DOI： 10.1117/1.jbo.25.1.014502

  • Saturated excitation microscopy using differential excitation for efficient detection of nonlinear fluorescence signals Reviewed

    Yasunori Nawa, Yasuo Yonemaru, Atsushi Kasai, Ryosuke Oketani, Hitoshi Hashimoto, Nicholas I. Smith, Katsumasa Fujita

    APL Photonics   3 ( 8 )   080805 - 080805   2018.8

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    Language：Others   Publishing type：Research paper (scientific journal)  

    DOI： 10.1063/1.5039567

  • High-resolution imaging in two-photon excitation microscopy using in situ estimations of the point spread function Reviewed

    Atsushi Doi, Ryosuke Oketani, Yasunori Nawa, Katsumasa Fujita

    Biomedical Optics Express   9 ( 1 )   202 - 213   2018.1

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    Language：Others   Publishing type：Research paper (scientific journal)  

    DOI： 10.1364/boe.9.000202

  • Saturated two-photon excitation fluorescence microscopy with core-ring illumination Reviewed

    Ryosuke Oketani, Atsushi Doi, Nicholas I. Smith, Yasunori Nawa, Satoshi Kawata, Katsumasa Fujita

    Optics Letters   42 ( 3 )   571 - 574   2017.2

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    We demonstrated resolution improvement in two-photon excitation microscopy by combining saturated excitation (SAX) of fluorescence and pupil manipulation. We theoretically estimated the resolution improvement and the sidelobe effect in the point spread function with various pupil designs and found that the combination of SAX and core-ring illumination can effectively enhance the spatial resolution in 3D and suppress sidelobe artifacts. The experimental demonstration shows that the proposed technique is effective for observation with a depth of 100 μm in a tissue phantom and can be applied to 3D observations of tissue samples with higher spatial resolution than conventional two-photon excitation microscopy.

    DOI： 10.1364/ol.42.000571

  • Study of Nonlinear Plasmonic Scattering in Metallic Nanoparticles Reviewed

    Yu-Ting Chen, Po-Hsuan Lee, Po-Ting Shen, Jann Launer, Ryosuke Oketani, Kuan-Yu Li, Yen-Ta Huang, Kyoko Masui, Satoru Shoji, Katsumasa Fujita, Shi-Wei Chu

    ACS Photonics   3 ( 8 )   1432 - 1439   2016.8

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    Nonlinear optical effects play key roles to communication, sensing, imaging, and so on. Recently, nonlinear scattering (saturation and reverse saturation) was discovered in gold nanospheres, providing a novel approach to nonbleaching super-resolution microscopy. However, the nonlinearity was previously limited to green-orange plasmonic band. It is highly desirable to extend the applicable wavelength range. In this work, we demonstrated nonlinear scattering in near-infrared with gold nanorods and in blue-violet with silver nanospheres. Besides, the nonlinear mechanism is clarified via different material/geometry. By spectrally decoupling the contributions of plasmonic absorption/scattering and interband/intraband transitions, we have verified plasmonic absorption, and the subsequent thermal effects to be the dominating source of nonlinearity. Our work not only provides the physical mechanism of the nonlinear scattering, but also paves the way toward multicolor super-resolution imaging based on plasmonic scattering.

    DOI： 10.1021/acsphotonics.6b00025

  • Ultrasmall all-optical plasmonic switch and its application to superresolution imaging Reviewed

    Hsueh-Yu Wu, Yen-Ta Huang, Po-Ting Shen, Hsuan Lee, Ryosuke Oketani, Yasuo Yonemaru, Masahito Yamanaka, Satoru Shoji, Kung-Hsuan Lin, Chih-Wei Chang, Satoshi Kawata, Katsumasa Fujita, Shi-Wei Chu

    Scientific Reports   6 ( 1 )   24293   2016.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/srep24293

  • Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle Reviewed

    Hsuan Lee, Kuan-Yu Li, Yen-Ta Huang, Po-Ting Shen, Gitanjal Deka, Ryosuke Oketani, Yasuo Yonemaru, Masahito Yamanaka, Katsumasa Fujita, Shi-Wei Chu

    Journal of Visualized Experiments   ( 107 )   e53338   2016.1

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    DOI： 10.3791/53338

  • Point spread function analysis with saturable and reverse saturable scattering Reviewed

    Hsuan Lee, Ryosuke Oketani, Yen-Ta Huang, Kuan-Yu Li, Yasuo Yonemaru, Masahito Yamanaka, Satoshi Kawata, Katsumasa Fujita, Shi-Wei Chu

    Optics Express   22 ( 21 )   26016 - 26022   2014.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1364/oe.22.026016

  • Saturation and Reverse Saturation of Scattering in a Single Plasmonic Nanoparticle Reviewed

    Shi-Wei Chu, Hsueh-Yu Wu, Yen-Ta Huang, Tung-Yu Su, Hsuan Lee, Yasuo Yonemaru, Masahito Yamanaka, Ryosuke Oketani, Satoshi Kawata, Satoru Shoji, Katsumasa Fujita

    ACS Photonics   1 ( 1 )   32 - 37   2014.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1021/ph4000218

  • Measurement of a Saturated Emission of Optical Radiation from Gold Nanoparticles: Application to an Ultrahigh Resolution Microscope Reviewed

    Shi-Wei Chu, Tung-Yu Su, Ryosuke Oketani, Yen-Ta Huang, Hsueh-Yu Wu, Yasuo Yonemaru, Masahito Yamanaka, Hsuan Lee, Guan-Yu Zhuo, Ming-Ying Lee, Satoshi Kawata, Katsumasa Fujita

    Physical Review Letters   112 ( 1 )   017402   2014.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1103/physrevlett.112.017402

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Presentations

  • Sheet-activation-based Super-Resolution Microscopy for Imaging Thick Biological Samples Invited

    桶谷亮介, 天満健太, 藤田克昌

    日本顕微鏡学会第81回学術講演会  2025.6 

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    Event date： 2025.6

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

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  • Bessel ビームを用いた multiplex CARS 顕微鏡の色収差補償

    桶谷 亮介, 田中 京介, 加納 英明

    2023年度日本分光学会年次講演会  2023.10 

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    Event date： 2023.10

    Language：Japanese  

    Country：Other  

  • 超解像イメージング：飽和したプラズモン光散乱の利用

    桶谷 亮介, Tung-Yu Su, 米丸 泰央, Hsuan Lee, 山中 真仁, Ming-Ying Lee, 河田 聡, Shi-Wei Chu, 藤田 克昌

    第60回応用物理学会春季学術講演会  2013.3 

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    Language：Japanese  

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  • Development of super-resolution microscopy by means of saturable plasmon scattering of metal nano particles

    Ryosuke Oketani, Tung-Yu Su, Yasuo Yonemaru, Hsuan Lee, Masahito Yamanaka, Ming-Ying Lee, Satoshi Kawata, Shi-Wei Chu, Katsumasa Fujita

    NCS-JST Workshop on Nano Devices  2013.3 

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  • Super-resolution Imaging of Gold Nanoparticles Based on Saturation of Plasmonic Light Scattering

    Ryosuke Oketani, Tung-Yu Su, Yasuo Yonemaru, Hsuan Lee, Masahito Yamanaka, Ming-Ying Lee, Satoshi Kawata, Shi-Wei Chu, Katsumasa Fujita

    2013 Conference on Lasers and Electro-Optics Pacific Rim  2013.7 

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  • Super-Resolution Microscopy by Using Saturation of Plasmonic Light Scattering

    Ryosuke Oketani, Tung-Yu Su, Yasuo Yonemaru, Hsuan Lee, Masahito Yamanaka, Ming-Ying Lee, Satoshi Kawata, Shi-Wei Chu, Katsumasa Fujita

    Asia Student Photonics Conference 2013  2013.7 

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  • Spatial Resolution Boosting for Confocal Microscopy by Using Saturation of Plasmonic Light Scattering

    Ryosuke Oketani, Tung-Yu Su, Masahito Yamanaka, YasuoYonemaru, Yen-Ta Huang, Hsueh-Yu Wu, Hsuan Lee, Guan-Yu Zhuo, Ming-Ying Lee, Satoshi Kawata, Shi-Wei Chu, Katsumasa Fujita

    Optics & Photonics Taiwan the International Conference 2013  2013.12 

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  • 金ナノ粒子の散乱光飽和を用いた超解像イメージング

    Ryosuke Oketani, Tung-Yu Su, Yasuo Yonemaru, Hsuan Lee, Masahito Yamanaka, Ming-Ying Lee, Satoshi Kawata, Shi-Wei Chu, Katsumasa Fujita

    応用物理学会関西支部 平成25年度第3回講演会「関西からのフォトニクスとカーボン研究イノベーション」  2014.2 

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    Language：Japanese  

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  • Nonlinear Scattering from Metallic Nanoparticles And its Application to Super Resolution Imaging

    Ryosuke Oketani, Tung-Yu-Su, Hsueh-Yu Wu, Hsuan Lee, Yen-Ta Huang, Yasuo Yonemaru, Satoshi Kawata, Shi-Wei Chu, Katsumasa Fujita

    Asia Student Photonics Conference 2014  2014.7 

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  • Super-Resolution Microscopy by Using Saturation of Plasmonic Light Scattering

    Ryosuke Oketani, Tung-Yu Su, Yasuo Yonemaru, Hsuan Lee, Masahito Yamanaka, Ming-Ying Lee, Satoshi Kawata, Shi-Wei Chu, Katsumasa Fujita

    6th Vietnamese-Japanese Students’ Scientific Exchange Meeting  2014.9 

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  • Nonlinear Scattering from Gold Nanoparticles And its Application to High Resolution Imaging

    Ryosuke Oketani, Tung-Yu-Su, Hsueh-Yu Wu, Hsuan Lee, Yen-Ta Huang, Yasuo Yonemaru, Satoshi Kawata, Shi-Wei Chu, Katsumasa Fujita

    International OSA Network of Students 5 Hokkaido  2014.9 

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  • Modification of Point-Spread Function in Confocal Microscopy by Nonlinear Plasmonic Light Scattering

    Ryosuke Oketani, Hsueh-Yu Wu, Hsuan Lee, Yen-Ta Huang, Yasuo Yonemaru, Tung-Yu Su, Satoshi Kawata, Shi-Wei Chu, Katsumasa Fujita

    JSAP-OSA Joint Symposia 2014  2014.9 

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  • Point-Spread Function of Confocal Microscopy Modified by Nonlinear Plasmonic Light Scattering

    Ryosuke Oketani, Hsueh-Yu Wu, Hsuan Lee, Yen-Ta Huang, Yasuo Yonemaru, Tung-Yu Su, Satoshi Kawata, Shi-Wei Chu, Katsumasa Fujita

    Japan-Singapore Workshop on Nanophotonics, Plasmonics, and Metamaterials  2014.12 

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  • Modification of Point-Spread Function in Confocal Imaging by Using Nonlinear Light Scattering

    Ryosuke Oketani, Hsueh-Yu Wu, Hsuan Lee, Yen-Ta Huang, Yasuo Yonemaru, Tung-Yu Su, Satoshi Kawata, Shi-Wei Chu, Katsumasa Fujita

    Optics & Photonics Taiwan the International Conference 2014  2014.12 

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  • High-Resolution Fluorescence Imaging by Saturated Two-Photon Excitation with Core-Ring Illumination

    Ryosuke Oketani, Atsushi Doi, Nicholas I. Smith, Yasunori Nawa, Satoshi Kawata, Katsumasa Fujita

    The 24th General Congress of the International Commission for Optics  2017.8 

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  • High-Resolution Fluorescence Imaging by Saturated Two-Photon Excitation with Core-Ring Illumination

    Ryosuke Oketani, Atsushi Doi, Nicholas I. Smith, Yasunori Nawa, Satoshi Kawata, Katsumasa Fujita

    INP-2017, The international Nanophotonics Symposium  2017.8 

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  • Super-Resolution in Two-Photon Excitation Microscopy via Saturated Excitation

    Ryosuke Oketani, Atsushi Doi, Nicholas I. Smith, Yasunori Nawa, Satoshi Kawata, Katsumasa Fujita

    JSAP-OSA Joint Symposia 2017  2017.9 

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  • 2光子励起の飽和を利用した超解像イメージング

    光子励起の飽和を利用した超解像イメージング, 桶谷亮介, 土井厚志, ミヤサキ ケートリン, 久保俊貴, 名和靖矩, 河田 聡, 藤田克昌

    Optics & Photonics Japan 2017  2017.11 

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  • 2光子励起の飽和とCore-ring照明を利用した 超解像イメージング

    桶谷亮介, 土井厚志, Nicholas I. Smith, 名和靖矩, 河田聡, 藤田克昌

    第43回レーザ顕微鏡研究会＆シンポジウム  2018.1 

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  • 2 光子励起とその飽和を用いた超解像蛍光イメージング

    桶谷亮介, 土井厚志, ミヤサキケートリン, 久保俊貴, 名和靖矩, 河田聡, 藤田克昌

    電気学会 電子・情報・システム部門 光・量子デバイス研究会  2018.3 

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    Language：Japanese  

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  • 可視光を用いた 2 光子励起顕微鏡による 3 次元生細胞イメージング

    桶谷亮介, 上垣久美子, 久保俊貴, 松田知己, スミスニコラス, 永井健治, 藤田克昌

    Optics & Photonics Japan 2018  2018.11 

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    Language：Japanese  

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  • 2 光子励起とその飽和を用いた超解像蛍光イメージング Invited

    桶谷亮介

    平成 30 年 電子・情報・システム部門 優秀論文発表賞 A 賞，研究会奨励賞，技術委員会奨励賞 受賞記念講演会  2019.3 

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  • 可視光を用いた2光子励起多点走査顕微鏡による 3 次元生細胞イメージング

    桶谷亮介, 須田悠, 上垣久美子, 久保俊貴, 松田知己, 山中真仁, 新井由之, Nicholas I, Smith, 永井健治, 藤田克昌

    レーザ顕微鏡研究会 第44回講演会  2019.7 

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  • Not so long time ago in my student life, filed away…. Invited

    Ryosuke Oketani

    Student Conference On Light  2019.11 

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  • スピニングディスク共焦点蛍光顕微鏡の線型および可視域二光子励起における結像特性

    須田悠, 桶谷亮介, 藤田克昌

    Optics & Photonics Japan 2019  2019.12 

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  • Besselビーム照明による色収差補正を用いたmultiplex非線形ラマンイメージング

    桶谷 亮介, 田中 京介, 加納 英明

    第84回応用物理学会秋季学術講演会  2023.9 

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  • Selective plane activation for super-resolution imaging inside volumetric samples Invited International conference

    Ryosuke Oketani

    SPIE Photonics West 2025  2025.1  SPIE

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    Language：English   Presentation type：Oral presentation (invited, special)  

    Country：United States  

  • 非線形照明パターンを用いた光学顕微鏡の開発 Invited

    桶谷亮介

    第18 回 先端的レーザー分光の若手シンポジウム  2024.12 

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    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Country：Japan  

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MISC

  • ラマン分光イメージング法の高速化技術 Invited

    桶谷亮介

    分光研究   74 ( 5 )   118 - 120   2025

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    Authorship：Lead author,　Corresponding author   Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

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  • Super-Resolution Microscopy with Nonlinear Fluorescence Responses for Signal Localization Invited

    Oketani Ryosuke, Temma Kenta, Fujita Katsumasa

    KENBIKYO   60 ( 2 )   51 - 56   2025   （ ISSN:13490958 eISSN:24342386 ）

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)   Publisher：The Japanese Society of Microscopy  

    <p>Nonlinear optical responses of specimens enable super-resolution microscopy by confining the excitation and detection volumes of fluorescence to spatial regions smaller than the diffraction limit. These responses arise under specific illumination conditions, such as increased light intensity or specific excitation wavelengths, which induce non-linear emission behaviors in fluorescent molecules. This review highlights laser-scanning and light-sheet microscopy as key platforms where nonlinear optical effects have been applied to significantly enhance spatial resolution. In addition to these platforms, structured illumination microscopy (SIM), an established super-resolution technique, can further broaden its capabilities by incorporating nonlinear optical responses—a direction also highlighted in this review. Selective-plane activation SIM (SPA-SIM), for instance, integrates light-sheet illumination and structured illumination using photoswitchable fluorescent proteins, enabling the selective observation of specific planes with high spatial resolution. This approach effectively suppresses background signals and enables three-dimensional super-resolution imaging even in thick or dense specimens, such as cell spheroids.</p>

    DOI： 10.11410/kenbikyo.60.2_51

    CiNii Research

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  • 応用物理学会学生チャプターは，暗い部屋に集まってコソコソ活動しているわけじゃない！

    桶谷亮介

    応用物理   2015.2

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    Language：Japanese  

Professional Memberships

Committee Memberships

  • 日本光学会   「光学」編集委員  

    2024.4 - Present   

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    Committee type：Academic society

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Research Projects

  • Development of Super-Resolution Nonlinear Raman Imaging Method for 3D Label-Free Observation of Biomolecules

    Grant number：24K17623  2024.4 - 2026.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Early-Career Scientists

    桶谷 亮介

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    Grant type：Scientific research funding

    本研究では、3次元観察が可能で標識が不要な顕微鏡を開発し、その空間分解能を分子レベルにまで高めるための基盤となる技術開発と結像特性の理論的・実験的検証を目的とする。具体的には、無標識3次元観察法の1つである非線形ラマン顕微鏡に、申請者がこれまで開発してきた超解像技術を導入し、 空間分解能の向上とその結像特性を評価する。また、スペクトル検出を同時に行うことで、 試料の網羅的な無標識分子同定を可能にする。非線形ラマンのコヒーレント特性を最大限活用可能な手法と理論を確立し、新たな生体内分子観察技術の礎を築く。

    CiNii Research

  • 脳波のコヒーレンスを生み出す細胞間協同作用のin-vivoラベルフリー可視化

    Grant number：21H04961  2021 - 2023

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

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    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

  • コヒーレントラマン分光と信号飽和を利用した観察対象を指定しない超解像顕微鏡の開発

    Grant number：21K20505  2021 - 2022

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Research Activity start-up

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • 金属ナノ粒子におけるプラズモン光散乱の非線形特性解明とその光学機器への応用

    Grant number：15J01780  2015 - 2016

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for JSPS Fellows

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    Authorship：Principal investigator  Grant type：Scientific research funding

Award for Educational Activities

  • 大阪大学工学研究科長表彰

    2015.3   大阪大学  

    Award-winner：第５回こども科学の教室「スーパー光塾～君も光博士になろう～」学生スタッフ代表

  • 大阪大学工学研究科長表彰

    2014.3   大阪大学  

    Award-winner：第４回こども科学の教室「スーパー光塾～君も光博士になろう～」学生スタッフ代表

Class subject

  • 化学序説

    2025.4 - 2026.3   Full year

  • 物理化学実験

    2024.12 - 2025.2   Winter quarter

  • 物理化学実験

    2023.12 - 2024.2   Winter quarter

  • 化学序説

    2023.4 - 2024.3   Full year

  • 物理化学実験

    2022.12 - 2023.2   Winter quarter

  • 物理化学実験

    2021.12 - 2022.2   Winter quarter

  • 化学序説（R3年度入学者及び2年次未進級者用）

    2021.4 - 2022.3   Full year

  • 化学序説（R2年度以前入学者用）

    2021.4 - 2021.9   First semester

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FD Participation

  • 2022.7   Role：Participation   Title：【化学科FD】電子ジャーナルに関するFD

    Organizer：Undergraduate school department

  • 2021.4   Role：Participation   Title：2021年度新任教員教育セミナー

    Organizer：University-wide

  • 2021.4   Role：Participation   Title：2021年度・第1回全学ＦＤ（新任教員の研修）

    Organizer：University-wide

Social Activities

  • いろんな虹を作って調べよう！

    Role(s)：Lecturer

    福岡市立有田小学校  科学クラブ  2026.1

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    Type：Visiting lecture

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