Faculty Profiles - IMAFUKU YASUHIRO

Information

写真a

IMAFUKU YASUHIRO

Organization

Faculty of Science Department of Biology Assistant Professor
School of Sciences Department of Biology（Concurrent）
Graduate School of Systems Life Sciences Department of Systems Life Sciences（Concurrent）

Contact information

Profile

研究：タンパク質分子モーターの作動原理の解明を目指す生物物理学的研究。

Homepage

http://www.biology.kyushu-u.ac.jp/~funccell/

External link

Degree

PhD (Kyushu University, Japan)

Research Interests・Research Keywords

Research theme： Study of short term synaptic plasticity

Keyword： Neurobiology

Research period： 2014.4
Research theme： Biophysical study on molecular motor proteins

Keyword： molecular motor

Research period： 1998.9

Awards

第１５回加藤記念研究助成

2004.3 加藤記念バイオサイエンス研究振興財団

Papers

Novel Distinctive Roles of Docking Proteins in Short-term Synaptic Plasticity of Frog Neuromuscular Transmission Revealed by Botulinum Neurotoxins Reviewed

Yasuhiro Imafuku, Koh ichi Enomoto, Hiroko Kataoka, Isao Ito, Takashi Maeno

Neuroscience 369 374 - 385 2018.1

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Short-term synaptic plasticity (SSP) is a basic mechanism for temporal processing of neural information in synaptic transmission. Facilitation, the fastest component of SSP, has been extensively investigated with regard to Ca²⁺ signaling and other relevant substances. However, systematic analyses on the slower components of SSP, originated by Magleby and Zengel, have remained stagnant for decades, as few chemicals directly modifying these slower components have been identified. In combination with refined experimental protocols designed to study the stimulation frequency-dependence of SSP and botulinum neurotoxins A and C (BoNT-A and BoNT-C), we investigated SSP of frog neuromuscular transmission to clarify the roles of synaptosomal-associated protein of 25 kDa (SNAP-25) and syntaxin, SNARE proteins exclusively participating in vesicular events including docking, priming and exocytosis. We found that BoNT-A treatment eliminated slow potentiation, and BoNT-C poisoning abolished intermediate augmentation, two components of SSP. Fast facilitation was maintained after double poisoning with BoNT-A and -C, but the postsynaptic response became biphasic. A novel depression, termed repression, emerged by double poisoning. Repression was different from depletion because it developed even at a low-frequency stimulation of 1 Hz. We conclude that SNAP-25 and syntaxin not only play roles as cooperative exocytotic machinery, but also have roles in SSP.

DOI： 10.1016/j.neuroscience.2017.11.022
Effect of elastic energy on the folding of an RNA hairpin Reviewed

Neil Thomas, Yasuhiro Imafuku

Journal of Theoretical Biology 312 96 - 104 2012.11

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We analyse the folding and unfolding of an RNA hairpin using a conventional zipping model that includes both the free energy for RNA binding and the elastic free energy of the system. Unfolding under isotonic conditions (where we control the applied load) is known to occur at a well-defined critical load. In marked contrast, we find that unfolding under isometric conditions (where we control the extension of the hairpin) produces a series of sharp peaks in the average load as the stem of the hairpin starts to unzip base by base. A peak occurs when the elastic energy stored in the unzipped arms of the hairpin becomes so large that it is energetically favourable for the next base pair to unzip: the consequent increase in the contour length of the unzipped arms reduces their elastic energy and causes the average load to fall abruptly. However, as the contour length of the unzipped arms increases, the peaks become less distinct. Moreover, when we include the long DNA/RNA handles that have been used in single-molecule experiments, the unzipping of individual base pairs cannot be resolved at all. Instead, with the hairpin in the folded state, the average load increases with extension until the elastic energy stored in the handles makes it energetically favourable for the hairpin to unzip over a narrow range of extensions. The resultant yield point produces a mechanical hysteresis loop with a negative slope, as observed experimentally. Unfolding of the hairpin is also affected by the elastic energy stored in a compliant force transducer. We find that short, stiff handles and a stiff force transducer could improve the resolution of mechanical experiments on single molecules.

DOI： 10.1016/j.jtbi.2012.07.021
Hopping and stalling of processive molecular motors Reviewed

Yasuhiro Imafuku, Neil Thomas, Katsuhisa Tawada

Journal of Theoretical Biology 261 ( 1 ) 43 - 49 2009.11

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When a two-headed molecular motor such as kinesin is attached to its track by just a single head in the presence of an applied load, thermally activated head detachment followed by rapid re-attachment at another binding site can cause the motor to 'hop' backwards. Such hopping, on its own, would produce a linear force-velocity relation. However, for kinesin, we must incorporate hopping into the motor's alternating-head scheme, where we expect it to be most important for the state prior to neck-linker docking. We show that hopping can account for the backward steps, run length and stalling of conventional kinesin. In particular, although hopping does not hydrolyse ATP, we find that the hopping rate obeys the same Michaelis-Menten relation as the ATP hydrolysis rate. Hopping can also account for the reduced processivity observed in kinesins with mutations in their tubulin-binding loop. Indeed, it may provide a general mechanism for the breakdown of perfect processivity in two-headed molecular motors.

DOI： 10.1016/j.jtbi.2009.07.011
Direction and speed of microtubule movements driven by kinesin motors arranged on catchin thick filaments Reviewed

Toshiki Taba, Masaki Edamatsu, Shiori Toba, Keitaro Shibata, Yasuhiro Imafuku, Yoko Yano Toyoshima, Katsuhisa Tawada, Akira Yamada

Cytoskeleton 65 ( 10 ) 816 - 826 2008.10

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Conventional kinesin (Kinesin-1) is a microtubule-based molecular motor that supports intracellular vesicle/organelle transport in various eukaryotic cells. To arrange kinesin motors similarly to myosin motors on thick filaments in muscles, the motor domain of rat conventional kinesin (amino acid residues 1-430) fused to the C-terminal 829 amino acid residues of catchin (KHC430Cat) was bacterially expressed and attached to catchin filaments that can attach to and arrange myosin molecules in a bipolar manner on their surface. Unlike the case of myosin where actin filaments move toward the center much faster than in the opposite direction along the catchin filaments, microtubules moved at the same speed in both directions. In addition, many microtubules moved across the filaments at the same speed with various angles between the axes of the microtubule and catchin filament. Kinesin/catchin chimera proteins with a shorter kinesin neck domain were also prepared. Those without the whole hinge 1 domain and the C-terminal part of the neck helix moved microtubules toward the center of the catchin filaments significantly, but only slightly, faster than in the opposite direction, although the movements in both directions were slower than those of the KHC430Cat construct. The results suggest that kinesin has substantial mechanical flexibility within the motor domain, possibly within the neck linker, enabling its interaction with microtubules having any orientation.

DOI： 10.1002/cm.20303
Anomalous fluctuations in sliding motion of cytoskeletal filaments driven by molecular motors Model simulations Reviewed

Yasuhiro Imafuku, Namiko Mitarai, Katsuhisa Tawada, Hiizu Nakanishi

Journal of Physical Chemistry B Materials 112 ( 5 ) 1487 - 1493 2008.2

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It has been found in in vitro experiments that cytoskeletal filaments driven by molecular motors show finite diffusion in sliding motion even in the long filament limit [Imafuku, Y. et al. Biophys. J. 1996, 70, 878-886. Noda, N. et al. Biophysics 2005, 1, 45-53]. This anomalous fluctuation can be evidence for cooperativity among the motors in action because fluctuation should be averaged out for a long filament if the action of each motor is independent. In order to understand the nature of the fluctuation in molecular motors, we perform numerical simulations and analyze velocity correlation in three existing models that are known to show some kind of cooperativity and/or large diffusion coefficient, i.e., the Sekimoto-Tawada model [Sekimoto, K.; Tawada, K. Phys. Rev. Lett. 1995, 75, 180], the Prost model [Prost, J. et al. Phys. Rev. Lett. 1994, 72, 2652], and the Duke model [Duke, T. Proc. Natl. Acad. Sci. U.S.A. 1999, 96, 2770]. It is shown that the Prost model and the Duke model do not give a finite diffusion in the long filament limit, in spite of the collective action of motors. On the other hand, the Sekimoto-Tawada model has been shown to give a diffusion coefficient that is independent of filament length, but it comes from the long time correlation whose time scale is proportional to filament length, and our simulations show that such a long correlation time conflicts with the experimental time scales. We conclude that none of the three models represent experimental findings. In order to explain the observed anomalous diffusion, we have to search for a mechanism that will allow both the amplitude and the time scale of the velocity correlation to be independent of the filament length.

DOI： 10.1021/jp074838l
Fluctuations in sliding motion of cytoskeltal filament driven by molecular motors Reviewed International journal

Y. Imafuku, N. Mitarai, K. Tawada, and H. Nakanishi

IEEE MHS2007 & Micro-Nano COE Vol.1, pp193-198 (2006). 2007.11

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Fluctuation of actin sliding over myosin thick filaments in vitro Reviewed International journal

N. Noda, Y. Imafuku, A. Yamada & K. Tawada

IEEE MHS2006 & Micro-Nano COE Vol.1, pp460-465 (2006). 2006.11

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Fluctuation of actin sliding over myosin thick filaments in vitro Reviewed International journal

Naoki Noda, Yasuhiro Imafuku, Akira Yamada and Katsuhisa Tawada

Biophysics Vol.1, pp45-53 (2005). 2005.1

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Kinesin: a molecular motor with a spring in its step Reviewed International journal

Neil Thomas, Yasuhiro Imafuku & Katsuhisa Tawada

P ROY SOC LOND B BIO 269 (1507): 2363-2371 269 ( 1507 ) 2363 - 2371 2002.11

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DOI： 10.1098/rspb.2002.2117
Molecular motors: thermodynamics and the random walk Reviewed International journal

Neil Thomas, Yasuhiro Imafuku & Katsuhisa Tawada

P ROY SOC LOND B BIO 268 (1481): 2113-2122 268 ( 1481 ) 2113 - 2122 2001.10

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分子モータの滑り速度とタンパク質分子摩擦 Reviewed

太和田勝久、今福泰浩

トライボロジスト 45 (2) : 138-144 2000.1

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Language：Japanese
A protein friction model of the actin sliding movement generated by myosin in mixtures of MgATP and MgGTP in vitro Reviewed International journal

Yasuhiro Imafuku, Yumiko Emoto & Katsuhisa Tawada

J THEOR BIOL 199 (4): 359-370 199 ( 4 ) 359 - 370 1999.8

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DOI： 10.1006/jtbi.1999.0963
Synchronous firing patterns of a set of insect neurosecretory cells Reviewed International journal

Toshio Ichikawa, Yasuhiro Imafuku & Katsuhisa Tawada

NEUROSCI LETT 264 (1-3): 85-88 APR 2 1999 264 ( 1-3 ) 85 - 88 1999.4

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DOI： 10.1016/S0304-3940(99)00164-0
Fluctuations in sliding motion of cytoskeltal filament driven by molecular motors

Yasuhiro Imafuku, Namiko Mitarai, Katsuhisa Tawada, Hiizu Nakanishi

2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS 193 - 198 2007

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It has been found in several in vitro experiments that cytoskeletal filaments driven by molecular motors show finite diffusion in sliding movement even in the long filament limit (Imafuku et al., 1996a, 1997; Noda et al., 2005). This anomalous fluctuation can be evidence that there exists some cooperativity among the motors in action because fluctuation should be averaged out if the action of each motor is independent. In order to understand the nature of the cooperativity in existing models of molecular motors, we perform numerical simulations and analyze velocity correlation on three models that are known to show some kind of cooperativity and/or large diffusion coefficient in the long filament limit. It is shown that Prost model (1994) and Duke model (1999) do not give a finite diffusion in the long filament limit in spite of collective action of motors. Quenched randomness in Sekimoto-Tawada model (1995) has been shown to give constant diffusion coefficient independent of filament length because of long time correlation proportional to filament length, but such a long correlation time is found to conflict with the experimental time scales. We conclude that none of the three models represent experimental findings, and a mechanism to be understood should allow both the amplitude and the time scale of the velocity correlation to be independent of the filament length.

DOI： 10.1109/MHS.2007.4420851
Fluctuation of actin sliding over myosin thick filaments in vitro

Naoki Noda, Yasuhiro Imafuku, Akira Yamada, Katsuhisa Tawada

2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS 2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS 2006

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It is customarily assumed that myosin motors act as independent force generators. Here, we tested this assumption by analyzing the fluctuation of the actin sliding movement in vitro over long native thick filaments from shell fish smooth muscle. This analysis is based on the prediction that the effective diffusion coefficient of actin, a measure of the fluctuation, is proportional to the inverse of the number of myosin motors generating the sliding movement of an actin filament, hence proportional to the inverse of the actin length, if the actions of the motors are independent. Contrary to this prediction, we found the effective diffusion coefficient to be independent of the actin length. This result shows that the myosin motors are not independent force generators when generating the continuous sliding movement of actin in vitro.

DOI： 10.1109/MHS.2006.320292
Themodynamic mechano-chemistry of single motor enzymes Reviewed

Katsuhisa Tawada, Yasuhiro Imafuku, Neil Thomas

Advances in Experimental Medicine and Biology 538 361 - 367 2004

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Molecular motors Thermodynamics and the random walk Reviewed

N. Thomas, Yasuhiro Imafuku, K. Tawada

Proceedings of the Royal Society B: Biological Sciences 268 ( 1481 ) 2113 - 2122 2001.10

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The biochemical cycle of a molecular motor provides the essential link between its thermodynamics and kinetics. The thermodynamics of the cycle determine the motor's ability to perform mechanical work, whilst the kinetics of the cycle govern its stochastic behaviour. We concentrate here on tightly coupled, processive molecular motors, such as kinesin and myosin V, which hydrolyse one molecule of ATP per forward step. Thermodynamics require that, when such a motor pulls against a constant load f, the ratio of the forward and backward products of the rate constants for its cycle is exp[-(ΔG + u₀f)/kT], where - ΔG is the free energy available from ATP hydrolysis and u₀ is the motor's step size. A hypothetical one-state motor can therefore act as a chemically driven ratchet executing a biased random walk. Treating this random walk as a diffusion problem, we calculate the forward velocity v and the diffusion coefficient D and we find that its randomness parameter r is determined solely by thermodynamics. However, real molecular motors pass through several states at each attachment site. They satisfy a modified diffusion equation that follows directly from the rate equations for the biochemical cycle and their effective diffusion coefficient is reduced to D - v²τ, where τ is the time-constant for the motor to reach the steady state. Hence, the randomness of multistate motors is reduced compared with the one-state case and can be used for determining τ. Our analysis therefore demonstrates the intimate relationship between the biochemical cycle, the force - velocity relation and the random motion of molecular motors.

DOI： 10.1098/rspb.2001.1764
Fluctuation correlation in the sliding movement generated by protein motors in vitro Reviewed

K. Tawada, M. Toyoda, Yasuhiro Imafuku, A. Yamada

Advances in Experimental Medicine and Biology 453 47 - 51 1998

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The fluctuation in the sliding distance of cytoskeletal filaments driven to move by protein motors in vitro does not depend on the filament length. This is in sharp contrast to the case of Brownian movement of filamentous particles in their longitudinal directions, in which the positional fluctuation is proportional to the inverse of the length (L) of filaments. This latter 1/L dependence is a direct consequence of the central limit theorem: the statistical independence and randomness of the solvent molecule collisions with filaments, the collisions of which cause the random Brownian movement. The above length-independence in the sliding distance fluctuation found in the in vitro motility indicates the presence of correlation in the fluctuation. A possible explanation for the correlation is to assume that there is an extended time-correlation in the sliding movement, a correlation which could be produced by the actions on a sliding filament of protein motors with their heads randomly oriented in the in vitro motility assay system. We have checked this possibility by using long myosin thick filaments of molluscan smooth muscles, on which myosin heads are uniformly oriented, and have found that even with such myosin filaments with oriented myosin heads, the positional fluctuation of actin sliding distance does not depend on the actin filament length. This result thus indicates that the actions of protein motors on a sliding filament are not statistically independent or random, so that the positional fluctuation of filaments in the motor- generated sliding movement does not depend on the filament length.
Length dependence of displacement fluctuations and velocity in microtubule sliding movement driven by sea urchin sperm outer arm beta dynein in vitro Reviewed International journal

Yasuhiro Imafuku, Yoko Y. Toyoshima & Katsuhisa Tawada

Biophysical Chemistry 67 (1-3): 117-125 67 ( 1-3 ) 117 - 125 1997.9

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DOI： 10.1016/S0301-4622(97)00028-8
Monte Carlo study for fluctuation analysis of the in vitro motility driven by protein motors Reviewed International journal

Yasuhiro Imafuku, Yoko Y. Toyoshima & Katsuhisa Tawada

Biophysical Chemistry 59 (1-2): 139-153 59 ( 1-2 ) 139 - 153 1996.3

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DOI： 10.1016/0301-4622(95)00123-9
Fluctuation in the microtubule sliding movement driven by kinesin in vitro Reviewed International journal

Yasuhiro Imafuku, Yoko Y. Toyoshima & Katsuhisa Tawada

Biophysical Journal 70 (2): 878-886 70 ( 2 ) 878 - 886 1996.2

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Fluctuation in the microtubule sliding movement driven by kinesin in vitro Reviewed

Yasuhiro Imafuku, Yoko Y. Toyoshima, Katsuhisa Tawada

Biophysical Journal 70 ( 2 I ) 878 - 886 1996.2

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We studied the fluctuation in the translational sliding movement of microtubules driven by kinesin in a motility assay in vitro. By calculating the mean-square displacement deviation from the average as a function of time, we obtained motional diffusion coefficients for microtubules and analyzed the dependence of the coefficients on microtubule length. Our analyses suggest that 1) the motional diffusion coefficient consists of the sum of two terms, one that is proportional to the inverse of the microtubule length (as the longitudinal diffusion coefficient of a filament in Brownian movement is) and another that is independent of the length, and 2) the length-dependent term decreases with increasing kinesin concentration. This latter term almost vanishes within the length range we studied at high kinesin concentrations. From the length-dependence relationship, we evaluated the friction coefficient for sliding microtubules. This value is much larger than the solvent friction and thus consistent with protein friction. The length independence of the motional diffusion coefficient observed at sufficiently high kinesin concentrations indicates the presence of correlation in the sliding movement fluctuation. This places significant constraint on the possible mechanisms of the sliding movement generation by kinesin motors in vitro.
A single-track fluctuation analysis in the sliding movement by protein motors in vitro Reviewed

K. Tawada, Yasuhiro Imafuku, Y. Y. Toyoshima

Biophysical Journal 68 ( 4 SUPPL. ) 1995

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Presentations

Novel Roles of SNARE Proteins in Short-term Synaptic Plasticity of Frog Neuromuscular Transmission

Yasuhiro Imafuku, Koh-ichi Enomoto, Hiroko Kataoka, Isao Ito, and Takashi Maeno

第41回日本神経科学大会 2018.7

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Event date： 2018.7

Language：English Presentation type：Oral presentation (general)

Venue：神戸コンベンションセンター Country：Japan
TNBS, a denaturing reagent, mimics the actions of Botulinum neurotoxin types A and C on the synaptic plasticity.

Yasuhiro Imafuku, Naoya Suzuki, Koh-ichi Enomoto, Hiroko Kataoka, Isao Ito, and Takashi Maeno

第42回日本神経科学大会 2019.7

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Event date： 2018.7

Language：English Presentation type：Oral presentation (general)

Venue：朱鷺メッセ Country：Japan
Novel Distinctive Roles of Docking Proteins in Short-term Synaptic Plasticity of Frog Neuromuscular Transmission

Yasuhiro Imafuku, Koh-ichi Enomoto, Hiroko Kataoka, Isao Ito, and Takashi Maeno

第55回日本生物物理学会年会 2017.9

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Event date： 2017.9

Language：English Presentation type：Oral presentation (general)

Venue：熊本大学 Country：Japan
ATP synthesis by elastically coupled molecular motors

Yasuhiro Imafuku, Nils Gustafsson and Neil Thomas

第52回日本生物物理学会年会 2014.9

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Event date： 2014.9

Language：English Presentation type：Oral presentation (general)

Venue：札幌コンベンションセンター Country：Japan

ATP synthesis by elastically coupled nanomotors
ATP synthesis by elastically coupled nanomotors

Yasuhiro Imafuku, Nils Gustafsson and Neil Thomas

第50回日本生物物理学会年会 2012.9

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Event date： 2012.9

Presentation type：Oral presentation (general)

Venue：名古屋大学・理学部 Country：Japan

ATP synthesis by elastically coupled nanomotors
RNA ヘアピンとキネシンネックリンカーの構造変化ダイナミクス

今福泰浩、ニール・トーマス

2011年生体運動研究合同班会議 2011.1

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Event date： 2011.1

Presentation type：Oral presentation (general)

Venue：大阪市立大学（大阪市住吉区） Country：Japan
Zipping dynamics of RNA hairpin and kinesin neck linker

Yasuhiro Imafuku and Neil Thomas

第48回日本生物物理学会念会 2010.9

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Event date： 2010.9

Presentation type：Oral presentation (general)

Venue：東北大学・川内北キャンパス（宮城県仙台市） Country：Japan

Zipping dynamics of RNA hairpin and kinesin neck linker
キネシンネックリンカーとRNA ヘアピンのダイナミックス

今福泰浩、ニール・トーマス

2010年生体運動研究合同班会議 2010.1

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Event date： 2010.1

Presentation type：Oral presentation (general)

Venue：中央大学理工学部（東京都文京区） Country：Japan
Dynamics of zipping the kinesin neck linker and an RNA hairpin

Yasuhiro Imafuku and Neil Thomas

第47回日本生物物理学会念会 2009.10

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Event date： 2009.10 - 2009.11

Presentation type：Oral presentation (general)

Venue：アスティとくしま（徳島県徳島市） Country：Japan

Dynamics of zipping the kinesin neck linker and an RNA hairpin
Hopping and Stalling of Processive Molecular Motors International conference

Yasuhiro Imafuku, Katsuhisa Tawada and Neil Thomas

6th Asian Biophysics Association (ABA) Symposium 2009.1

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Event date： 2009.1

Presentation type：Oral presentation (general)

Venue：Hong Kong University of Science and Technology

Hopping and Stalling of Processive Molecular Motors
Torsional Flexibility of Kinesin: Direction and Speed of Microtubule Movements Driven by Kinesin Motors Arranged on Catchin Filaments International conference

Toshiki Taba, Masaki Edamatsu, Shiori Toba, Keitaro Shibata, Yasuhiro Imafuku, Yoko Yano Toyoshima, Katsuhisa Tawada, and Akira Yamada

2nd International Symposium on Bio-nanosystems 2008.11

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Event date： 2008.11 - 2007.11

Presentation type：Oral presentation (general)

Venue：東京大学小柴ホール（東京都文京区） Country：Japan

Torsional Flexibility of Kinesin: Direction and Speed of Microtubule Movements Driven by Kinesin Motors Arranged on Catchin Filaments
Zipping model for kinesin neck-linker docking

Neil Thomas and Yasuhiro Imafuku

第46回日本生物物理学会年会 2008.12

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Presentation type：Oral presentation (general)

Venue：福岡国際会議場 Country：Japan
キネシン変異体ヘテロダイマーの運動特性とホッピング

今福泰浩、Neil Thomas、太和田勝久

生体運動合同班会議 2004.1

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Venue：東京大学駒場地区（東京都） Country：Japan
5. , “キネシン変異体ヘテロダイマーの運動特性とホッピング”, ,

今福泰浩、Neil Thomas、太和田勝久

生体運動合同班会議 2004.1

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Venue：東京大学駒場地区（東京都） Country：Japan
キネシンヘテロダイマーの運動速度、キネシン頭部の速い結合解離による「ホッピング」

今福泰浩、Neil Thomas、太和田勝久

第42回生物物理学会年会（演題番号3P173） 2004.12

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Venue：京都国際会館（京都市） Country：Japan
キネシン繊維に沿った微小管の運動；ネック部位を短くした場合

田場登志希、枝松正樹、豊島陽子、鳥羽栞、山田章、今福泰浩、太和田勝久

第43回生物物理学会年会（演題番号1P173） 2005.11

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Venue：札幌コンベンションセンター（札幌市） Country：Japan
多数の分子モーターの同期と協調；滑り運動の計算機シミュレーションの揺らぎ解析

今福泰浩、御手洗菜美子、中西秀、太和田勝久

生体運動合同班会議 2006.1

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Venue：東京大学農学部（東京都） Country：Japan
Fluctuation of actin sliding over myosin thick filament in vitro International conference

Naoki Noda, Yasuhiro Imafuku, Akira Yamada, and Katsuhisa Tawada

MHS 2006 and Micro-Nano COE 2006.11

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Country：Japan
Fluctuation in sliding movement driven by molecular motors: simulation study International conference

Yasuhiro Imafuku, Namiko Mitarai, Hiizu Nakanishi, and Katsuhisa Tawada,

EABS and BSJ 2006 2006.11

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Venue：沖縄コンベンションセンター（宜野湾市） Country：Japan
Fluctuation in sliding movement driven by molecular motors: computer simulation study International conference

Yasuhiro Imafuku, Namiko Mitarai, Hiizu Nakanishi and Katsuhisa Tawada

Alpbach Workshop on Molecular Motors 2007 2007.3

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Venue：Alpbach, Tirol, Austria. Country：Japan
Fluctuations in sliding motion of cytoskeltal filament driven by molecular motors International conference

Y. Imafuku, N. Mitarai, K. Tawada, and H. Nakanishi

MHS2007 & Micro-Nano COE 2007.11

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Presentation type：Oral presentation (general)

Venue：名古屋大学 Country：Japan
再構成キネシン繊維上での微小管滑り運動の方向と速度

田場登志希、枝松正樹、鳥羽栞、今福泰浩、豊島陽子、太和田勝久、山田章

第45回日本生物物理学会年会 2007.12

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Venue：パシフィコ横浜 Country：Japan
ミオシン繊維上でのアクチン繊維滑り運動揺らぎ解析

野田直紀、今福泰浩、山田章、太和田勝久

第40回日本生物物理学会年会 2002.11

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Presentation type：Oral presentation (general)

Venue：名古屋大学（名古屋市） Country：Japan
キネシン・ネックリンカーのドッキングと8nm未満ステップ

今福泰浩、N. Thomas、太和田勝久

第40回日本生物物理学会年会 2002.11

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Presentation type：Oral presentation (general)

Venue：名古屋大学（名古屋市） Country：Japan
ミオシン繊維に沿ったアクチン繊維滑り運動の揺らぎ解析-ミオシン分子間共同作用の解明を目指して

野田直紀、今福泰浩、太和田勝久

生体運動合同班会議 2003.1

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Presentation type：Oral presentation (general)

Venue：アクロス福岡（福岡市） Country：Japan
ミオシンV単分子運動の負荷依存性とサブステップサイズ

今福泰浩、Neil Thomas、太和田勝久

第41回生物物理学会年会 2003.9

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Presentation type：Oral presentation (general)

Venue：朱鷺メッセ（新潟市） Country：Japan
アクチン繊維・ミオシン繊維間の滑り運動揺らぎ解析：ミオシンのコヒーレントな運動

野田直紀、今福泰浩、山田章、太和田勝久

第41回日本生物物理学会年会 2003.9

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Presentation type：Oral presentation (general)

Venue：朱鷺メッセ（新潟市） Country：Japan
kinesin thick filament の作成

田場登志希、今福泰浩、豊島陽子、枝松正樹、山田章、太和田勝久

第41回日本生物物理学会年会 2003.9

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Presentation type：Oral presentation (general)

Venue：朱鷺メッセ（新潟市） Country：Japan
"Force-velocity relationships of heterodimeric kinesins" International conference

Yasuhiro Imafuku, Neil Thomas and Katsuhisa Tawada

COE International Conference 2003 "Molecular Mechanism of Intracellular Transports: The Roles of Kinesin and Dynein Superfamily Proteins" 2003.11

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Presentation type：Oral presentation (general)

Venue：箱根プリンスホテル（箱根町） Country：Japan
"Processive movement of the myosin V molecular motor"

Yasuhiro Imafuku, Neil Thomas, and Katsuhisa Tawada

31st Annual Meeting of the European Society of Muscle Research 2002.9

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Presentation type：Oral presentation (general)

Venue：De Werelt（オランダ・ルンテレン） Country：Netherlands
双頭キネシン１分子の一方向性滑り運動の計算機シミュレーション

今福泰浩、N. Thomas、太和田勝久

生体運動合同班会議 1998.1

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Presentation type：Oral presentation (general)

Venue：順天堂大学（東京都） Country：Japan
滑り運動の揺らぎ：Huxleyモデルの計算機シミュレーションと実験の比較

今福泰浩、豊田将史、山田章、太和田勝久

第36回日本生物物理学会年会 1998.10

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Presentation type：Oral presentation (general)

Venue：九州大学六本松キャンパス（福岡市） Country：Japan
キネシン歩行モデルの計算機シミュレーション：8nm未満ステップとその機構

今福泰浩、神谷力、N. Thomas、R.A. Thornhill、太和田勝久

生体運動合同班会議 1999.1

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Presentation type：Oral presentation (general)

Venue：名古屋市立大学医学部（名古屋市） Country：Japan
ミオシン繊維の上をすべるアクチン滑り運動の揺らぎ

今福泰浩、豊田将史、高田聡、山田章、太和田勝久

第37回日本生物物理学会年会 1999.10

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Presentation type：Oral presentation (general)

Venue：和光市市民文化センター（和光市） Country：Japan
キネシン歩行モデルの計算機シミュレーション

今福泰浩、神谷力、N. Thomas、R.A. Thornhill、太和田勝久

生体運動合同班会議 2000.1

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Presentation type：Oral presentation (general)

Venue：千里ライフサイエンスセンター（吹田市） Country：Japan
キネシンネックリンカーのドッキングが運動速度と力の関係を決めている

今福泰浩、N. Thomas、太和田勝久

第38回日本生物物理学会年会 2000.9

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Presentation type：Oral presentation (general)

Venue：東北大学川内北キャンパス（仙台市） Country：Japan
"A Neck-Linker Docking Model of the Kinesin Molecular Motor" International conference

Yasuhiro Imafuku, Neil Thomas, Tsutomu Kamiya, and Katsuhisa Tawada

Frontiers in Molecular Motors Research 2000.11

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Presentation type：Oral presentation (general)

Venue：淡路夢舞台国際会議場（兵庫県津名郡東浦町） Country：Japan
ネックリンカー・ドッキングに基づくキネシン歩行モデル

今福泰浩、N. Thomas、太和田勝久

生体運動合同班会議 2001.1

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Presentation type：Oral presentation (general)

Venue：早稲田大学（東京都） Country：Japan
ミオシンＶの歩行モデル

今福泰浩、N. Thomas、太和田勝久

第39回日本生物物理学会年会 2001.10

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Presentation type：Oral presentation (general)

Venue：大阪大学（吹田市） Country：Japan
歩行型タンパク質分子モーター：熱力学とランダムウォーク

太和田勝久、N. Thomas、今福泰浩

第39回日本生物物理学会年会 2001.10

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Presentation type：Oral presentation (general)

Venue：大阪大学（吹田市） Country：Japan
脊髄由来細胞増殖抑制因子存在下でＭ期に入った細胞が示す奇妙な運動

前田梨恵、江本由美子、今福泰浩、太和田勝久

第39回日本生物物理学会年会 2001.10

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Presentation type：Oral presentation (general)

Venue：大阪大学（吹田市） Country：Japan
キネシン様歩行型分子モーター１分子の動力学と熱力学とランダムウォーク

太和田勝久、今福泰浩、Neil Thomas

生体運動合同班会議 2002.1

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Presentation type：Oral presentation (general)

Venue：千葉大学（千葉市） Country：Japan

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Works

MeasureWin.exe

今福泰浩

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粒子運動の動画を観察する際に、粒子の位置を測定するソフトウェア。Microsoft Windows において使用可能。ただし、.NET 1.1 および DirectX9 が必要。開発には C# を使用した。

Professional Memberships

Biophysical Society of Japan
The Japan Neuroscience Society

Academic Activities

座長（Chairmanship）

第41回日本生物物理学会年会（ Japan ） 2003.9

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Type：Competition, symposium, etc.
シンポジウム企画および座長

生物物理学会年会（ Japan ） 2003.9

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Type：Competition, symposium, etc.

Number of participants：200

Research Projects

キネシン分子モーターとＲＮＡヘアピンの構造変化ダイナミクスと機能の関連

2011 - 2013

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

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Authorship：Principal investigator Grant type：Scientific research funding
キネシンネックリンカードッキングにおけるＡＴＰの役割：ＲＮＡヘアピンとの比較

2011 - 2012

Japan Society for the Promotion of Science・Ministry of Education, Culture, Sports, Science and Technology Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas

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Authorship：Principal investigator Grant type：Scientific research funding
再構成キネシン繊維上での微小管滑り運動の速度と揺らぎ

Grant number：19770132 2007 - 2009

Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

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Authorship：Principal investigator Grant type：Scientific research funding
天然の筋肉構造にヒントを得た再構成分子モーター繊維の作成と滑り運動の解析

Grant number：18048035 2006 - 2007

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Priority Areas

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Authorship：Principal investigator Grant type：Scientific research funding
多数の生物分子モーターが非線型相互作用することでおこるマクロな滑り運動の創発

2005

平成17年度稲盛財団研究助成

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Authorship：Principal investigator Grant type：Contract research
多数の分子モーターの非線形相互作用によるマクロな滑り運動の創発

Grant number：16770119 2004 - 2006

Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

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Authorship：Principal investigator Grant type：Scientific research funding
多数の生物分子モーターが非線型相互作用することでおこるマクロな滑り運動の創発

2004

加藤記念研究助成

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Grant type：Contract research
多数の生物分子モーターが非線型相互作用することでおこるマクロな滑り運動の創発

2003

笹川科学研究助成

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Authorship：Principal investigator Grant type：Contract research
タンパク質分子モーターの動作機構に関する理論的研究 International coauthorship

1996.4 - 2013.3

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Authorship：Coinvestigator(s)
精子鞭毛の高速微小振動における分子モーターのシンクロナイゼーション

Grants-in-Aid for Scientific Research 特定領域研究(A)特定領域研究(B)

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Authorship：Principal investigator Grant type：Scientific research funding

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Educational Activities

基礎生物物理学実験、生物物理学、生物学概論、などの講義を担当。

Class subject

生物学概論

2024.10 - 2025.3 Second semester
基礎生物物理学実習

2024.10 - 2025.3 Second semester
生物物理学

2024.4 - 2024.9 First semester
生物学概論

2023.10 - 2024.3 Second semester
基礎生物物理学実習

2023.10 - 2024.3 Second semester
生物物理学

2023.4 - 2023.9 First semester
生物学概論

2022.10 - 2023.3 Second semester
基礎生物物理学実習

2022.10 - 2023.3 Second semester
生物物理学

2022.4 - 2022.9 First semester
生物学概論

2021.10 - 2022.3 Second semester
基礎生物物理学実習

2021.10 - 2022.3 Second semester
生物物理学

2021.4 - 2021.9 First semester
基礎生物学概要

2020.10 - 2021.3 Second semester
基礎生物物理学実習

2020.10 - 2021.3 Second semester
生物物理学

2020.4 - 2020.9 First semester
基礎生物物理学実習

2019.10 - 2020.3 Second semester
基礎生物学概要

2019.10 - 2020.3 Second semester
情報生物学

2019.10 - 2020.3 Second semester
生物物理学

2019.10 - 2020.3 Second semester
基礎生物物理学実習

2018.10 - 2019.3 Second semester
基礎生物学概要

2018.10 - 2019.3 Second semester
情報生物学

2018.10 - 2019.3 Second semester
情報生物学

2017.10 - 2018.3 Second semester
基礎生物物理学実習

2017.10 - 2018.3 Second semester
基礎生物学概要

2017.10 - 2018.3 Second semester
基礎生物物理学実習

2016.10 - 2017.3 Second semester
基礎生物学概要

2016.10 - 2017.3 Second semester
情報生物学

2016.10 - 2017.3 Second semester
基礎生物物理学実習

2015.10 - 2016.3 Second semester
基礎生物学概要

2015.10 - 2016.3 Second semester
基礎生物物理学実習

2014.10 - 2015.3 Second semester
基礎生物学概要

2014.10 - 2015.3 Second semester
基礎生物物理学実習

2013.10 - 2014.3 Second semester
自然科学総合実験

2013.4 - 2013.9 First semester
コアセミナー

2013.4 - 2013.9 First semester
生体高分子学実習

2011.10 - 2012.3 Second semester
コアセミナー

2011.4 - 2011.9 First semester
自然科学総合実験

2011.4 - 2011.9 First semester
生体高分子学実習

2010.10 - 2011.3 Second semester
コアセミナー

2010.4 - 2010.9 First semester
自然科学総合実験

2010.4 - 2010.9 First semester
生体高分子学実習

2009.10 - 2010.3 Second semester
自然科学総合実験

2009.4 - 2009.9 First semester
コアセミナー

2009.4 - 2009.9 First semester
生体高分子学実習

2008.10 - 2009.3 Second semester
自然科学総合実験

2008.10 - 2009.3 Second semester
コアセミナー

2008.4 - 2008.9 First semester
自然科学総合実験

2007.10 - 2008.3 Second semester
生体高分子学実習

2007.10 - 2008.3 Second semester
コアセミナー

2007.4 - 2007.9 First semester
生物学演習

2006.10 - 2007.3 Second semester
生体高分子学実習

2006.10 - 2007.3 Second semester
生物学演習

2005.10 - 2006.3 Second semester
生体高分子学実習

2005.10 - 2006.3 Second semester
生物学演習

2004.10 - 2005.3 Second semester
生体高分子学実習

2004.10 - 2005.3 Second semester
生物学演習

2003.10 - 2004.3 Second semester
生体高分子学実習

2003.4 - 2003.9 First semester

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FD Participation

2023.3 Role：Participation Title：【生物学科】大学発明の出願・権利化に関するFD

Organizer：Undergraduate school department
2022.3 Role：Participation Title：【生物学科】入学者選抜試験に関するFD

Organizer：Undergraduate school department
2022.3 Role：Participation Title：リベラルサイエンス教育開発FD「今、教養教育に求められるもの：イギリス教養史にみる科学の受容から」

Organizer：[Undergraduate school/graduate school/graduate faculty]
2021.6 Role：Participation Title：理系研究室の運営技術 ― ラボラトリーマネジメントという考え方 ―

Organizer：Undergraduate school department
2020.12 Role：Participation Title：【オンライン配信】新型コロナウィルス感染拡大状況での学生のメンタルヘルス

Organizer：[Undergraduate school/graduate school/graduate faculty]
2020.9 Role：Participation Title：M2B学習支援システム講習会（オンライン開催）◇初級編・中級編◇10:00～12:00

Organizer：University-wide
2019.12 Role：Participation Title：緊急時の応急措置に関するＦＤ／ＳＤ～エピペン及びＡＥＤについて～

Organizer：[Undergraduate school/graduate school/graduate faculty]
2019.10 Role：Participation Title：（生物学科FD）科研費改革後の学術研究動向について

Organizer：Undergraduate school department
2004.3 Role：Participation Title：不明

Organizer：[Undergraduate school/graduate school/graduate faculty]

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Other educational activity and Special note

2021 Class Teacher 学部
2020 Class Teacher 学部
2013 Class Teacher 学部

Outline of Social Contribution and International Cooperation activities

なし

Social Activities

出前講義

山口県立下関西高等学校 2015.10

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Audience：Infants,　Schoolchildren,　Junior students,　High school students

Type：Seminar, workshop
進学セミナー

長崎県立長崎北高等学校 2009.11

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Audience：Infants,　Schoolchildren,　Junior students,　High school students

Type：Seminar, workshop
社会人講演会

福岡県立筑紫丘高等学校 2005.6

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Audience：Infants,　Schoolchildren,　Junior students,　High school students

Type：Seminar, workshop

Acceptance of Foreign Researchers, etc.

The University of Birmingham

Acceptance period： 2005.8 - 2005.9 （Period）：1 month or more

Nationality：United Kingdom

Business entity：Japan Society for the Promotion of Science
The University of Birmingham

Acceptance period： 2004.4 （Period）：1 month or more

Nationality：United Kingdom

Business entity：Foreign governments, foreign research institutes, international organizations
The University of Birmingham

Acceptance period： 2003.8 - 2003.12 （Period）：1 month or more

Nationality：United Kingdom

Business entity：Foreign governments, foreign research institutes, international organizations

Travel Abroad

2012.3

Staying countory name 1：United Kingdom Staying institution name 1：University of Birmingham, UK
2011.1 - 2011.2

Staying countory name 1：United Kingdom Staying institution name 1：University of Birmingham, UK
2010.8 - 2010.9

Staying countory name 1：United Kingdom Staying institution name 1：University of Birmingham, UK
2009.8 - 2009.9

Staying countory name 1：United Kingdom Staying institution name 1：University of Birmingham, UK
2008.8 - 2008.9

Staying countory name 1：United Kingdom Staying institution name 1：University of Birmingham, UK
2007.8 - 2007.9

Staying countory name 1：United Kingdom Staying institution name 1：University of Birmingham, UK
2007.3

Staying countory name 1：United Kingdom Staying institution name 1：University of Birmingham, UK

Staying countory name 2：Austria Staying institution name 2：Alpbach Congress Centre, Tirol, Austria
2006.2 - 2006.3

Staying countory name 1：United Kingdom Staying institution name 1：University of Birmingham, UK
2003.1 - 2003.2

Staying countory name 1：United Kingdom Staying institution name 1：University of Birmingham, UK

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