Updated on 2024/11/25

Information

 

写真a

 
OKUMOTO KANJI
 
Organization
Faculty of Science Department of Biology Assistant Professor
School of Sciences Department of Biology(Concurrent)
Graduate School of Systems Life Sciences Department of Systems Life Sciences(Concurrent)
Title
Assistant Professor
Contact information
メールアドレス
Tel
0926426341
Profile
Research: The system regulating homeostasis of peroxisomes, including the biogenesis, morphology, and metabolic functions. Peroxisome biogenesis and its dysfunction in human disorders. Educatuion: Two experimental classes (fundamental biological experiments and applied cell biology); A class of molecular cell biology. Social activity: "ESSP ver.2", the high-level scientific program for high school students.
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Degree

  • Studies on RING finger peroxins, Pex10p and Pex12p: cDNA cloning and functional analysis

Research Interests・Research Keywords

  • Research theme:Studies on peroxisome biogenesis and the homeostasis

    Keyword:Peroixsome, homeostasis, ubiquitin, phosphorylation

    Research period: 2003.2

Awards

  • 第17回井上研究奨励賞

    2000.12   井上科学振興財団  

Papers

  • Analysis of Peroxisome Biogenesis by Phos-Tag SDS-PAGE. Reviewed International journal

    Kanji Okumoto, Yukio Fujiki

    Methods in molecular biology (Clifton, N.J.)   2643   207 - 215   2023.3   ISSN:10643745

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    Language:English   Publishing type:Research paper (scientific journal)  

    Phos-tag, a selective phosphate-binding molecule, and Phos-tag-based methodologies have been developed to investigate the phosphoproteome. In various analytical techniques using Phos-tag derivatives, phosphate-affinity electrophoresis using Phos-tag acrylamide, called Phos-tag SDS-PAGE, enables separation of phosphorylated proteins with a slower migration from non-phosphorylated proteins in polyacrylamide gels. The procedures for Phos-tag SDS-PAGE are largely common to those for conventional SDS-PAGE, thus being readily available for all laboratories. Phos-tag SDS-PAGE is widely applied to quantitative analysis of the overall phosphorylation state depending on the number and/or sites of the phosphate group. Phos-tag SDS-PAGE has also been introduced to the field of peroxisome study, including oxidative stress-induced and mitosis-specific phosphorylation of Pex14, a central component of the translocation machinery complex for peroxisomal matrix proteins. Here, we describe a practical protocol for Phos-tag SDS-PAGE and its application to peroxisome biogenesis research.

    DOI: 10.1007/978-1-0716-3048-8_15

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  • Molecular insights into peroxisome homeostasis and peroxisome biogenesis disorders Reviewed International journal

    Yukio Fujiki, Kanji Okumoto, Masanori Honsho, Yuichi Abe

    Biochimica et Biophysica Acta (BBA) - Molecular Cell Research   1869 ( 11 )   119330 - 119330   2022.11   ISSN:0167-4889 eISSN:1879-2596

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    Language:Others   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.bbamcr.2022.119330

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  • Regulation of Myt1 kinase activity via its N-terminal region in Xenopus meiosis and mitosis. Reviewed International journal

    Yukito Aiba, Jihoon Kim, Arata Imamura, Kanji Okumoto, Nobushige Nakajo

    Cells and Development   169   203754   2022.3   ISSN:2667-2901

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    Language:English   Publishing type:Research paper (scientific journal)  

    Immature animal oocytes are naturally arrested at the first meiotic prophase (Pro-I), which corresponds to the G2 phase of the cell cycle. In Xenopus oocytes, Myt1 kinase phosphorylates and inactivates cyclin-dependent kinase 1 (Cdk1) at Pro-I, thereby preventing oocytes from entering meiosis I (MI) prematurely. Previous studies have shown that, upon resuming MI, Cdk1 and p90rsk, which is a downstream kinase of the Mos-MAPK pathway, in turn phosphorylate the C-terminal region of Myt1, to suppress its activity, thereby ensuring high Cdk1 activity during M phase. However, the roles of the N-terminal region of Myt1 during meiosis and mitosis remain to be elucidated. In the present study, we show that the N-terminal region of Myt1 participates in the regulation of Myt1 activity in the Xenopus cell cycle. In particular, we found that a short, conserved sequence in the N-terminal region, termed here as the PAYF motif, is required for the normal activity of Myt1 in oocytes. Furthermore, multiple phosphorylations by Cdk1 at the Myt1 N-terminal region were found to be involved in the negative regulation of Myt1. In particular, phosphorylations at Thr11 and Thr16 of Myt1, which are adjacent to the PAYF motif, were found to be important for the inactivation of Myt1 in the M phase of the cell cycle. These results suggest that in addition to the regulation of Myt1 activity via the C-terminal region, the N-terminal region of Myt1 also plays an important role in the regulation of Myt1 activity.

    DOI: 10.1016/j.cdev.2021.203754

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  • Peroxisome Biogenesis Disorders Reviewed

    Masanori Honsho, Kanji Okumoto, Shigehiko Tamura, Yukio Fujiki

    Advances in Experimental Medicine and Biology   45 - 54   2020.12

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    DOI: 10.1007/978-3-030-60204-8_4

  • Peroxisome: Metabolic Functions and Biogenesis Reviewed

    Kanji Okumoto, Shigehiko Tamura, Masanori Honsho, Yukio Fujiki

    Advances in Experimental Medicine and Biology   3 - 17   2020.12

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    DOI: 10.1007/978-3-030-60204-8_1

  • The peroxisome counteracts oxidative stresses by suppressing catalase import via Pex14 phosphorylation Reviewed International journal

    Kanji Okumoto, Mahmoud El Shermely, Masanao Natsui, Hidetaka Kosako, Ryuichi Natsuyama, Toshihiro Marutani, Yukio Fujiki

    eLife   9   2020.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.7554/elife.55896

  • Recent insights into peroxisome biogenesis and associated diseases Reviewed

    Yukio Fujiki, Yuichi Abe, Yuuta Imoto, Akemi J. Tanaka, Kanji Okumoto, Masanori Honsho, Shigehiko Tamura, Non Miyata, Toshihide Yamashita, Wendy K. Chung, Tsuneyoshi Kuroiwa

    Journal of Cell Science   133 ( 9 )   jcs236943   2020.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1242/jcs.236943

  • Systematic Identification of Regulators of Oxidative Stress Reveals Non-canonical Roles for Peroxisomal Import and the Pentose Phosphate Pathway Reviewed

    Cell Reports   30 ( 5 )   1417 - 1433.e7   2020.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.celrep.2020.01.013

  • A newly identified mutation in the PEX26 gene is associated with a milder form of Zellweger spectrum disorder Reviewed

    Akemi J. Tanaka, Kanji Okumoto, Shigehiko Tamura, Yuichi Abe, Yoel Hirsch, Liyong Deng, Joseph Ekstein, Wendy K. Chung, Yukio Fujiki

    Cold Spring Harbor Molecular Case Studies   5 ( 1 )   a003483 - a003483   2019.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1101/mcs.a003483

  • Dynamics of the nucleoside diphosphate kinase protein DYNAMO2 correlates with the changes in the global GTP level during the cell cycle of Cyanidioschyzon merolae Reviewed

    Yuuta Imoto, Yuichi Abe, Kanji Okumoto, Mio Ohnuma, Haruko Kuroiwa, Tsuneyoshi Kuroiwa, Yukio Fujiki

    Proceedings of the Japan Academy Series B: Physical and Biological Sciences   95 ( 2 )   75 - 85   2019.1

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    DOI: 10.2183/pjab.95.007

  • A newly isolated Pex7-binding, atypical PTS2 protein P7BP2 is a novel dynein-type AAA+ protein Reviewed

    Hajime Niwa, Yasuhiro Miyauchi-Nanri, Kanji Okumoto, Satoru Mukai, Kentaro Noi, Teru Ogura, Yukio Fujiki

    Journal of Biochemistry   164 ( 6 )   437 - 447   2018.12

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    DOI: 10.1093/jb/mvy073

  • Onsite GTP fuelling via DYNAMO1 drives division of mitochondria and peroxisomes Reviewed

    Yuuta Imoto, Yuichi Abe, Masanori Honsho, Kanji Okumoto, Mio Ohnuma, Haruko Kuroiwa, Tsuneyoshi Kuroiwa, Yukio Fujiki

    Nature Communications   9 ( 1 )   2018.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41467-018-07009-z

  • New splicing variants of mitochondrial Rho GTPase-1 (Miro1) transport peroxisomes Reviewed

    Kanji Okumoto, Tatsuaki Ono, Ryusuke Toyama, Ayako Shimomura, Aiko Nagata, Yukio Fujiki

    Journal of Cell Biology   217 ( 2 )   619 - 633   2018.2

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    DOI: 10.1083/jcb.201708122

  • Identification of peroxisomal protein complexes with PTS receptors, pex5 and pex7, in mammalian cells Reviewed

    Kanji Okumoto, Non Miyata, Yukio Fujiki

    Subcellular Biochemistry   287 - 298   2018.1

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    DOI: 10.1007/978-981-13-2233-4_12

  • Cell death or survival against oxidative stress Reviewed International journal

    Non Miyata, Kanji Okumoto, Yukio Fujiki

    Subcellular Biochemistry   463 - 471   2018.1

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    DOI: 10.1007/978-981-13-2233-4_20

  • BAK regulates catalase release from peroxisomes Reviewed

    Yukio Fujiki, Non Miyata, Satoru Mukai, Kanji Okumoto, Emily H. Cheng

    Molecular & Cellular Oncology   4 ( 3 )   2017.3

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  • The VDAC2-BAK axis regulates peroxisomal membrane permeability Reviewed

    Ken-ichiro Hosoi, Non Miyata, Satoru Mukai, Satomi Furuki, Kanji Okumoto, Emily H. Cheng, Yukio Fujiki

    Journal of Cell Biology   216 ( 3 )   709 - 722   2017.3

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    DOI: 10.1083/jcb.201605002

  • Localization of protein kinase NDR2 to peroxisomes and its role in ciliogenesis Reviewed

    Shoko Abe, Tomoaki Nagai, Moe Masukawa, Kanji Okumoto, Yuta Homma, Yukio Fujiki, Kensaku Mizuno

    Journal of Biological Chemistry   292 ( 10 )   4089 - 4098   2017.3

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    DOI: 10.1074/jbc.M117.775916

  • Blue native PAGE Applications to study peroxisome biogenesis Reviewed International journal

    Kanji Okumoto, Shigehiko Tamura, Yukio Fujiki

    Methods in Molecular Biology   1595   197 - 205   2017.1

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    DOI: 10.1007/978-1-4939-6937-1_18

  • Peroxisomal membrane and matrix protein import using a semi-intact mammalian cell system Reviewed International journal

    Kanji Okumoto, Masanori Honsho, Yuqiong Liu, Yukio Fujiki

    Methods in Molecular Biology   1595   213 - 219   2017.1

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    DOI: 10.1007/978-1-4939-6937-1_20

  • Generation of peroxisome-deficient somatic animal cell mutants Reviewed International journal

    Kanji Okumoto, Yukio Fujiki

    Methods in Molecular Biology   1595   319 - 327   2017.1

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    DOI: 10.1007/978-1-4939-6937-1_29

  • Defining the dynamin-based ring organizing center on the peroxisome-dividing machinery isolated from Cyanidioschyzon merolae Reviewed

    Yuuta Imoto, Yuichi Abe, Kanji Okumoto, Masanori Honsho, Haruko Kuroiwa, Tsuneyoshi Kuroiwa, Yukio Fujiki

    Journal of Cell Science   130 ( 5 )   853 - 867   2017.1

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    DOI: 10.1242/jcs.199182

  • Protein Import into Peroxisomes: the principles and methods of studying (version 2.0) Reviewed International journal

    Yukio Fujiki, Kanji Okumoto, Masanori Honsho

    Encyclopedia of Life Sciences   2015.4

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  • Peroxisome biogenesis in mammalian cells Reviewed

    Yukio Fujiki, Kanji Okumoto, Satoru Mukai, Masanori Honsho, Shigehiko Tamura

    Frontiers in Physiology   5   307   2014.8

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    DOI: 10.3389/fphys.2014.00307

  • Distinct Modes of Ubiquitination of Peroxisome-targeting Signal Type 1 (PTS1) Receptor Pex5p Regulate PTS1 Protein Import Reviewed

    Kanji Okumoto, Hiromi Noda, Yukio Fujiki

    Journal of Biological Chemistry   289 ( 20 )   14089 - 14108   2014.5

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    DOI: 10.1074/jbc.m113.527937

  • Molecular Basis for Peroxisome Biogenesis Disorders Reviewed

    Y. Fujiki, K. Okumoto, S. Mukai, S. Tamura

    Molecular Machines Involved in Peroxisome Biogenesis and Maintenance   91 - 110   2014.5

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    DOI: 10.1007/978-3-7091-1788-0_5

  • System to quantify the import of peroxisomal matrix proteins by fluorescence intensity Reviewed International journal

    Masafumi Noguchi, Kanji Okumoto, Yukio Fujiki

    GENES TO CELLS   18 ( 6 )   476 - 492   2013.6

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    DOI: 10.1111/gtc.12051

  • Pex5p stabilizes Pex14p: a study using a newly isolated pex5 CHO cell mutant, ZPEG101 Reviewed International journal

    Ryuichi Natsuyama, Kanji Okumoto, Yukio Fujiki

    Biochem J.   449 ( 1 )   195 - 207   2013.1

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    DOI: 10.1042/BJ20120911.

  • AWP1/ZFAND6 functions in Pex5 export by interacting with cys-monoubiquitinated Pex5 and Pex6 AAA ATPase Reviewed International journal

    Traffic   13 ( 1 )   168 - 183   2012.1

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    DOI: 10.1111/j.1600-0854.2011.01298.x.

  • New insights into dynamic and functional assembly of the AAA peroxins, Pex1p and Pex6p, and their membrane receptor Pex26p in shuttling of PTS1-receptor Pex5p during peroxisome biogenesis Reviewed International journal

    Biochim. Biophys. Acta   1823 ( 1 )   2012.1

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    DOI: 10.1111/j.1600-0854.2011.01298.x.

  • Two Proteases, Trypsin Domain-containing 1 (Tysnd1) and Peroxisomal Lon Protease (PsLon), Cooperatively Regulate Fatty Acid β-Oxidation in Peroxisomal Matrix Reviewed

    286 ( 52 )   44367 - 44379   2011.12

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    DOI: 10.1074/jbc.m111.285197

  • Cysteine-ubiquitination of peroxisome-targeting-signal type 1 (PTS1)-receptor Pex5p regulates Pex5p import and export Reviewed International journal

    Kanji Okumoto, Sachi Misono, Non Miyata, Yui Matsumoto, Satoru Mukai, Yukio Fujiki

    Traffic   2011.5

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    DOI: 10.1111/j.1600-0854.2011.01217.x

  • Lessons from peroxisome-deficient Chinese hamster ovary (CHO) cell mutants Reviewed

    Yukio Fujiki, Kanji Okumoto, Naohiko Kinoshita, Kamran Ghaedi

    Biochimica et Biophysica Acta (BBA) - Molecular Cell Research   1763 ( 12 )   1374 - 1381   2006.12

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    DOI: 10.1016/j.bbamcr.2006.09.012

  • cDNA cloning and characterization of the third isoform in human peroxin Pex11p. Reviewed International journal

    Biochem. Biophys. Res. Commun.   300 ( 4 )   819 - 823   2003.1

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    DOI: 10.1016/S0006-291X(02)02936-4

  • Molecular Anatomy of the Peroxin Pex12p: RING Finger domain is essential for Pex12p function and interacts with the peroxisome-targeting signal type 1-receptor Pex5p and a RING Peroxin, Pex10p Reviewed

    Kanji Okumoto, Isao Abe, Yukio Fujiki

    Journal of Biological Chemistry   275 ( 33 )   25700 - 25710   2000.8

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    DOI: 10.1074/jbc.m003303200

  • The peroxin Pex3p initiates membrane assembly in peroxisome biogenesis. Reviewed International journal

    Mol. Biol. Cell   11 ( 6 )   2085 - 2102   2000.6

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  • Peroxisome biogenesis and molecular defects in peroxisome assembly disorders Reviewed International journal

    Cell Biochem. Biophys.   32 ( 1 )   155 - 164   2000.4

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    DOI: 10.1385/CBB:32:1-3:155

  • Isolation and characterization of novel peroxisome biogenesis-defective Chinese hamster ovary cell mutants using green fluorescent protein. Reviewed International journal

    Exp, Cell Res.   248 ( 2 )   489 - 497   1999.5

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    DOI: 10.1006/excr.1999.4413

  • The peroxin Pex14p, cDNA cloning by functional complementation on a Chinese hamster ovary cell mutant, characterization, and functional analysis. Reviewed International journal

    J. Biol. Chem   274 ( 18 )   12593 - 12604   1999.4

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    DOI: 10.1074/jbc.274.18.12593

  • Mutations in PEX10 is the cause of Zellweger peroxisome deficiency syndrome of complementation group B Reviewed

    K Okumoto, R Itoh, N Shimozawa, Y Suzuki, S Tamura, N Kondo, Y Fujiki

    Human Molecular Genetics   7 ( 9 )   1399 - 1405   1998.9

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    DOI: 10.1093/hmg/7.9.1399

  • Newly identified Chinese hamster ovary cell mutants are defective in biogenesis of peroxisomal membrane vesicles (peroxisomal ghosts), representing a novel complementation group in mammals. Reviewed International journal

    J. Biol. Chem.   273 ( 37 )   24122 - 24130   1998.9

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    DOI: 10.1074/jbc.273.37.24122

  • PEX12 , the Pathogenic Gene of Group III Zellweger Syndrome: cDNA Cloning by Functional Complementation on a CHO Cell Mutant, Patient Analysis, and Characterization of Pex12p Reviewed

    Kanji Okumoto, Nobuyuki Shimozawa, Atsusi Kawai, Shigehiko Tamura, Toshiro Tsukamoto, Takashi Osumi, Hugo Moser, Ronald J. A. Wanders, Yasuyuki Suzuki, Naomi Kondo, Yukio Fujiki

    Molecular and Cellular Biology   18 ( 7 )   4324 - 4336   1998.7

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    DOI: 10.1128/mcb.18.7.4324

  • Clofibrate-inducible, 28-kDa peroxisomal integral membrane protein is encoded by PEX11. Reviewed International journal

    FEBS Lett.   431 ( 3 )   468 - 472   1998.7

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    DOI: 10.1016/S0014-5793(98)00815-1

  • Human PEX1 cloned by functional complementation on a CHO cell mutant is responsible for peroxisome-deficient Zellweger syndrome of complementation group I. Reviewed International journal

    Proc. Natl. Acad. Sci. USA.   95 ( 8 )   4350 - 4355   1998.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.95.8.4350

  • Peroxisome biogenesis disorders: identification of a new complementation group distinct from peroxisome-deficient CHO mutants and not complemented by human PEX13. Reviewed International journal

    Biochem. Biophys. Res. Commun.   243 ( 2 )   368 - 371   1998.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1006/bbrc.1997.8067

  • Peroxisome targeting signal type 1 (PTS1) receptor is involved in import of both PTS1 and PTS2: studies with PEX5-defective CHO cell mutants. Reviewed International journal

    Mol. Cell. Biol.   18 ( 1 )   388 - 399   1998.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/mcb.18.1.388

  • PEX12 encodes an integral membrane protein of peroxisomes Reviewed

    Kanji Okumoto, Yukio Fujiki

    Nature Genetics   17 ( 3 )   265 - 266   1997.11

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    DOI: 10.1038/ng1197-265

  • Newly identified Chinese hamster ovary cell mutants defective in peroxisome biogenesis represent two novel complementation groups in mammals Reviewed

    Keita Tateishi, Kanji Okumoto, Nobuyuki Shimozawa, Toshiro Tsukamoto, Takashi Osumi, Yasuyuki Suzuki, Naomi Kondo, Ichiro Okano, Yukio Fujiki

    European Journal of Cell Biology   73 ( 4 )   352 - 359   1997.9

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  • Isolation and characterization of peroxisome-deficient Chinese hamster ovary cell mutants representing human complementation group III Reviewed

    Kanji Okumoto, Akemi Bogaki, Keita Tateishi, Toshiro Tsukamoto, Takashi Osumi, Nobuyuki Shimozawa, Yasuyuki Suzuki, Tadao Orii, Yukio Fujiki

    Experimental Cell Research   233 ( 1 )   11 - 20   1997.5

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    DOI: 10.1006/excr.1997.3552

  • Isolation of a new peroxisome-deficient CHO cell mutant defective in peroxisome targeting signal-1 receptor Reviewed

    Toshiro Tsukamoto, Akemi Bogaki, Kanji Okumoto, Keita Tateishi, Yukio Fujiki, Nobuyuki Shimozawa, Yasuyuki Suzuki, Naomi Kondo, Takashi Osumi

    Biochemical and Biophysical Research Communications   230 ( 2 )   402 - 406   1997.1

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    DOI: 10.1006/bbrc.1996.5971

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Presentations

  • Pex14のリン酸化を介したペルオキシソームへのカタラーゼ輸送の抑制と細胞の酸化ストレス耐性応答

    奥本寛治、小迫英尊、藤木幸夫

    第43回日本分子生物学会年会  2020.12 

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    Event date: 2020.12

    Language:Japanese  

    Venue:オンライン   Country:Japan  

  • 過酸化水素分解酵素カタラーゼの細胞内輸送の抑制は細胞の酸化ストレス応答として機能する

    奥本寛治、小迫英尊、藤木幸夫

    令和2年度日本生化学会九州支部例会  2020.5 

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    Event date: 2020.5

    Language:Japanese  

    Venue:九州大学病院キャンパス、福岡   Country:Japan  

  • H2O2-induced phosphorylation of Pex14p suppresses peroxisomal import of catalase to counteract oxidative stress. Invited

    2019.12 

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    Event date: 2019.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • ペルオキシソーム局在性テイルアンカー型タンパク質ACBD5の翻訳アレストを介した局在化効率獲得

    #小山 桂恵奈, 奥本 寛治, 田村 茂彦, 藤木 幸夫

    第42回日本分子生物学会年会  2019.12 

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    Event date: 2019.12

    Language:Japanese  

    Venue:福岡国際会議場、福岡   Country:Japan  

  • ペルオキシソーム局在性テイルアンカー型膜タンパク質の輸送局在化機構

    奥本 寛治, 田村 茂彦, 八木田 悠一, 本庄 雅則, 藤木 幸夫

    第41回日本分子生物学会年会  2018.11 

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    Event date: 2018.11

    Language:Japanese  

    Venue:パシフィコ横浜、神奈川   Country:Japan  

  • Biogenesis of peroxisomal tail-anchored membrane proteins, new splicing variants of Miro1, acyl-CoA binding domain containing protein 5 (ACBD5), and peroxin Pex26p. International conference

    Kanji Okumoto, Yuichi Yagita, Masanori Honsho, and Yukio Fujiki

    2018.8 

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    Event date: 2018.8

    Language:English  

    Country:Japan  

  • ペルオキシソームの機能と細胞内配置・運動性の新たな制御機構 Invited

    奥本寛治, #小野立晃, 外山隆介, #細井謙一郎, 宮田暖, 向井悟, Cheng E. H., Yukio Fujiki

    2017年度生命科学系学会合同年次大会  2017.12 

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    Event date: 2017.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:神戸国際会議場   Country:Japan  

  • ペルオキソソームの恒常性:膜およびマトリックスタンパク質輸送の分子基盤 Invited

    第17回日本蛋白質科学会年会  2017.6 

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    Event date: 2017.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:仙台国際会議場(宮城)   Country:Japan  

  • ペルオキシソーム局在型Miro1バリアントによるペルオキシソームの細胞内移動制御

    奥本寛治, #小野立晃, #下村紋子, 外山隆介, Yukio Fujiki

    第89回日本生化学会大会  2016.9 

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    Event date: 2016.9

    Language:Japanese  

    Venue:仙台国際センター   Country:Japan  

  • ペルオキシソーム移行シグナル1型(PTS1)レセプターPex5pの結合因子、P5BP1の機能解析

    第66回日本細胞生物学会大会  2014.6 

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    Event date: 2014.6

    Language:Japanese  

    Venue:東大寺総合文化センター(奈良市)   Country:Japan  

  • RING peroxin complexes comprising Pex10p and Pex12p ubiquitinate the PTS1 receptor Pex5p and regulate its shuttling between peroxisomes and the cytosol. International conference

    Kanji Okumoto, Hiromi Noda, Yukio Fujiki

    PerFuMe Kick-off Conference  2013.12 

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    Event date: 2013.12

    Language:English  

    Country:Netherlands  

    RING peroxin complexes comprising Pex10p and Pex12p ubiquitinate the PTS1 receptor Pex5p and regulate its shuttling between peroxisomes and the cytosol.

  • ペルオキシソーム移行シグナル1型(PTS1)レセプターPex5pの新規結合因子、P5BP1の同定

    第86回日本生化学会大会  2013.9 

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    Event date: 2013.9

    Language:Japanese  

    Venue:パシフィコ横浜(横浜市)   Country:Japan  

  • Pex14pの安定性に関わるPTS1レセプターPex5pの新たな機能:新規PEX5欠損CHO変異細胞ZPEG101を用いた解析

    第85回日本生化学会大会  2012.12 

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    Event date: 2012.12

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    Venue:福岡国際会議場(福岡市)   Country:Japan  

  • PTS1レセプターPex5pは複数種のユビキチン化修飾を受け、ペルオキシソームマトリクスタンパク質輸送を制御する

    35回日本分子生物学会年会  2012.12 

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    Event date: 2012.12

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    Venue:福岡国際会議場(福岡市)   Country:Japan  

  • Pex14リン酸化を介したカタラーゼの細胞内局在制御による新規酸化ストレス応答機構 International conference

    奥本寛治, 藤木幸夫

    第44回日本分子生物学会年会  2021.12 

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    Language:Others   Presentation type:Symposium, workshop panel (public)  

    Country:Other  

  • カタラーゼの局在制御を介したストレス応答機構を調節するリン酸化シグナル伝達経路 International conference

    奥本寛治, 藤木幸夫

    第95回日本生化学会大会  2022.11 

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    Language:Others  

    Venue:名古屋国際会議場   Country:Japan  

  • ペルオキシソームを介した細胞の酸化ストレス応答機構 Invited International conference

    奥本寛治, 藤木幸夫

    第96回日本生化学会大会  2023.11 

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    Language:Others   Presentation type:Symposium, workshop panel (public)  

    Venue:福岡国際会議場   Country:Japan  

  • 過酸化水素分解酵素カタラーゼの細胞内局在制御による酸化ストレス応答の分子機構

    藤木幸夫、奥本寛治、宮田暖、Emily Cheng

    第14回日本臨床ストレス応答学会  2019.11 

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    Event date: 2019.11

    Language:Japanese  

    Venue:大阪市立大学、大阪   Country:Japan  

  • ペルオキシソーム⽋損症病因遺伝⼦PEX26の新規変異同定とその障害の分⼦メカニズム

    ⽥村茂彦, 奥本寛治, Tanaka Akemi J., 阿部雄⼀, Hirsch Yoel, Deng Liyong, Ekstein Joseph, Chung Wendy K., 藤⽊幸夫

    第92回⽇本⽣化学会⼤会  2019.9 

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    Event date: 2019.9

    Language:Japanese  

    Venue:パシフィコ横浜、神奈川   Country:Japan  

  • Local GTP fueling via nucleoside diphosphate kinase like protein DYNAMO1 drives division of peroxisome and mitochondrion. Invited International conference

    Yukio Fujiki, Yuuta Imoto, Yuichi Abe, Masanori Honsho, Kanji Okumoto, Mio Ohnuma, Haruko Kuroiwa, and Tsuneyoshi Kuroiwa

    ASCB/EMBO 2018 Meeting  2018.12 

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    Event date: 2018.12

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:United States  

  • テイルアンカー型タンパク質ACBD5 の 翻訳速度とペルオキシソームへの輸送・局在化効率の関連性の検討

    #小山桂恵奈, 奥本寛治, 田村茂彦, 藤木幸夫

    平成30年度日本生化学会九州支部例会  2018.6 

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    Event date: 2018.6 - 2018.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:九州大学、福岡   Country:Japan  

  • VDAC2-BAK axis regulates peroxisomal membrane permeability and catalase release. Invited International conference

    Yukio Fujiki, Miyata, N., Mukai, S.,Hosoi, K., Okumoto, K., and Cheng, E.H.

    Gordon Research Conference on Organellar Channels & Transporters  2017.7 

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    Event date: 2017.7

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:United States  

  • NDR2 のペルオキシソーム膜への局在と一次繊毛形成における役割 Invited

    増川萌瑛 , 阿部彰子, 永井友朗, 奥本寛治, 本間悠太, 藤木幸夫, 水野健作

    第69回日本細胞生物学会大会  2017.6 

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    Event date: 2017.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:仙台国際会議場(宮城)   Country:Japan  

  • Identification of a novel protein interacting with peroxisome targeting signal type 1 (PTS1) receptor, Pex5p.

    Yuri Shirahama, Kanji Okumoto, Yukio Fujiki

    2013.12 

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    Event date: 2013.12

    Language:Japanese  

    Country:Japan  

    Identification of a novel protein interacting with peroxisome targeting signal type 1 (PTS1) receptor, Pex5p.

  • Two proteases, trypsin domain-containing 1 (Tysnd1) and peroxisomal Lon protease (PsLon), cooperatively regulate fatty acid β-oxidation in peroxisomal matrix

    奥本寛治、亀谷紫、藤木幸夫

    第45回日本発生生物学会、第64回日本細胞生物学会合同年会  2012.5 

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    Event date: 2012.5

    Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    Venue:神戸国際会議場(神戸市)   Country:Japan  

  • PTS1レセプターPex5pのペルオキシソーム-細胞質間リサイクリングはシステイン残基のユビキチン化修飾により制御される

    奥本寛治、美園紗知、宮田暖、松元由依、向井悟、藤木幸夫

    第35回日本分子生物学会年会  2011.12 

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    Event date: 2011.12

    Venue:パシフィコ横浜(横浜市)   Country:Japan  

  • A Novel Function of AWP1/ZFAND6: Regulation of Pex5p Export by Interacting with Cys-monoubiquitinated Pex5p and AAA ATPase, Pex6p International conference

    The American Society for Cell Biology Annual Meeting 2011  2011.12 

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    Event date: 2011.12

    Country:United States  

    A Novel Function of AWP1/ZFAND6: Regulation of Pex5p Export by Interacting with Cys-monoubiquitinated Pex5p and AAA ATPase, Pex6p

  • PTS1レセプターPex5pのユビキチン化修飾によるペルオキシソームマトリクスタンパク質輸送の制御

    奥本寛治、藤木幸夫

    平成23年度日本生化学会九州支部例会  2011.5 

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    Event date: 2011.5

    Presentation type:Oral presentation (general)  

    Venue:久留米大学医学部(久留米市)   Country:Japan  

  • Functional roles of two serine proteases, Tysnd1 and PsLon, in peroxisome matrix International conference

    The 3rd International Symposium on Protein Community  2010.9 

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    Event date: 2010.9

    Country:Japan  

  • Characterization of peroxisome membrane protein TMEM135/ PMP52. International conference

    2010.5 

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    Event date: 2010.5

    Country:Japan  

  • The complex of RING peroxins, Pex10p and Pex12p, functions as an ubiquitin ligase for the PTS1 receptor Pex5p and regulates peroxisomal matrix protein import. International conference

    2009.12 

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    Event date: 2009.12

    Country:Japan  

  • Domain mapping of RING peroxins essential for peroxisomal localization and interaction with peroxisome targeting signal type-1 receptor Pex5p International conference

    2006.6 

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    Country:Japan  

  • Novel function of peroxisome targeting signal type-1 receptor Pex5p in Pex14p stability: study using a newly isolated peroxisome-deficient CHO mutant, ZPEG101. International conference

    2007.5 

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    Country:Japan  

  • Construction and Characterization of a Dominant-Negative Mutant of Peroxisome Targeting Signal Type-1 Receptor Pex5p International conference

    2007.12 

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    Country:United States  

  • ペルオキシソーム移行シグナル1型レセプターPex5pのドミナントネガティブ変異体を用いたペルオキシソームマトリクスタンパク質輸送機構の解析 International conference

    奥本寛治、藤木幸夫

    第30回日本分子生物学会年会・第80回日本生化学会 合同大会  2007.12 

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    Venue:パシフィコ横浜(横浜市)   Country:Japan  

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MISC

  • ペルオキシソームの動態と機能制御研究の新展開 Reviewed

    奥本 寛治, 阿部 雄一, 藤木 幸夫

    生化学   2023.12

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • ペルオキシソームの輸送と分解を基盤とした恒常性制御機構

    奥本 寛治, 藤木 幸夫

    2018.12

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    Language:Japanese  

  • オルガネラ間の協奏によるペルオキシソームの機能と恒常性の制御

    藤木幸夫, 奥本寛治, 本庄雅則

    2018.11

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    Language:Japanese  

  • ペキソファジー:ペルオキシソームの形成・機能制御と分解機構

    藤木 幸夫, 山下俊一, 奥本 寛治, 本庄 雅則

    2017.7

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    Language:Japanese  

  • Molecular basis for peroxisome biogenesis disorders

    Molecular machines involved in peroxisome biogenesis and maintenance, Springer-Verlag, Wien, Austria. pp. 91-110   2014.10

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    Language:English   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • ペルオキシソームの形成・制御とその障害

    藤木 幸夫, 宮田 暖, 奥本 寛治, 田村 茂彦, 糸山 彰徳, 本庄 雅則

    2012.10

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    Language:Japanese  

  • ペルオキシソームの形成・制御の分子基盤

    2011.11

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    Language:Japanese  

  • ペルオキシソームの形成障害と分子病態 (共著)

    藤木幸夫, 奥本寛治, 田村茂彦

    実験医学   2003.9

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    Language:Japanese  

  • ペルオキシソームの形成とその異常症(共著)

    藤木幸夫, 奥本寛治

    蛋白質核酸酵素   2000.4

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    Language:Japanese  

  • 【細胞小器官の形成,活性調節およびストレス応答研究の新展開】ペルオキシソームの動態と機能制御研究の新展開

    奥本 寛治, 阿部 雄一, 藤木 幸夫

    生化学   95 ( 6 )   719 - 729   2023.12   ISSN:0037-1017

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    Authorship:Lead author   Language:Japanese   Publisher:(公社)日本生化学会  

    ペルオキシソームは極長鎖脂肪酸のβ酸化をはじめとした多様かつ重要な代謝機能を有する細胞小器官(オルガネラ)である.ペルオキシソーム形成に必須な多数のペルオキシン遺伝子(PEX)の同定,およびその翻訳産物であるペルオキシンの機能解析が大きく進展し,ペルオキシソーム欠損症の全病因PEX遺伝子の解明に続いてペルオキシソームの形成機構が明らかとなってきた.ここでは,ペルオキシソーム構成タンパク質の輸送局在化やペルオキシソームの分裂,形態制御,分解などの統合的制御によるペルオキシソーム形成機構を概説し,ペルオキシソームタンパク質の輸送局在化機構や酸化ストレス応答におけるペルオキシソームの役割に関する最新の知見について,哺乳類ペルオキシソームを中心に我々の成果を紹介する.(著者抄録)

  • Molecular basis of local energy generation during mitochondrial and peroxisomal division Reviewed

    Imoto Y., Abe Y., Honsho M., Okumoto K., Ohnuma M., Kuroiwa H., Kuroiwa T., and Fujiki Y.

    2021.3

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    DOI: 10.5685/plmorphol.32.59

  • Protein Import into Peroxisomes: the principles and methods of studying (version 2.0)

    Yukio Fujiki, Kanji Okumoto, Masanori Honsho

    Encyclopedia of Life Sciences   2015.4

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    Language:Others  

  • Isolation and characterization of three newly identified complementation groups of peroxisome-deficient mammalian cell mutants.

    Y Fujiki, K Okumoto, K Tateishi, T Tsukamoto, T Osumi, N Shimozawa, Y Suzuki

    MOLECULAR BIOLOGY OF THE CELL   1996.12

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    Language:English  

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Professional Memberships

  • The Molecular Biology Society of Japan

  • The Japanese Biochemistry Society

  • Japan Society for Cell Biology

Academic Activities

  • 実行委員 International contribution

    第85回日本生化学会大会  ( 福岡国際会議場(福岡市) ) 2012.12

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    Type:Competition, symposium, etc. 

    Number of participants:4,000

  • 実行委員

    第40回日本発生生物学会・第59回日本細胞生物学会合同大会  ( 福岡国際会議場(福岡市) ) 2007.5 - Present

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    Type:Competition, symposium, etc. 

    Number of participants:2,000

  • 組織委員

    第28回日本分子生物学会  ( 福岡市 福岡ドーム ) 2005.12 - Present

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    Type:Competition, symposium, etc. 

    Number of participants:10,000

Research Projects

  • リン酸化修飾が制御するペルオキシソームタンパク質輸送と酸化ストレス応答

    Grant number:21K06155  2021 - 2024

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 過酸化水素分解酵素カタラーゼの細胞内局在制御による酸化ストレス応答の分子機構の解明

    2018

    日本応用酵素協会 酵素研究助成 2018年度

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    Authorship:Principal investigator  Grant type:Contract research

  • リン酸化修飾を介したペルオキシソーム恒常性制御

    Grant number:17K07310  2017 - 2019

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • ペルオキシソーム–小胞体接触領域の形成機構とその生理機構

    2016

    QRプログラム わかばチャレンジ

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    Authorship:Principal investigator  Grant type:On-campus funds, funds, etc.

  • リン酸化シグナル伝達によるペルオキシソーム形成調節機構

    Grant number:26440032  2014 - 2016

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 抗細胞老化物質の探索を目指した、カタラーゼのペルオキシソーム輸送効率測定法の確立

    2013

    科学技術振興機構 研究成果展開事業研究成果最適展開支援プログラム(A-STEP)

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    Authorship:Principal investigator  Grant type:Contract research

  • システイン残基修飾を介したペルオキシソームタンパク質輸送の制御

    Grant number:24770130  2012 - 2013

    科学研究費助成事業  若手研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • ユビキチン化修飾によるペルオキシソームマトリクスタンパク質輸送の調節機構

    Grant number:21770117  2009 - 2010

    科学研究費助成事業  若手研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • ペルオキシソームの形成・制御と障害

    Grant number:19058011  2007 - 2011

    科学研究費助成事業  特定領域研究

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • ユビキチン化修飾によるペルオキシソーム形成機構の制御

    Grant number:19770087  2007 - 2008

    科学研究費助成事業  若手研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 細胞内小器官ペルオキシソームのマトリックスタンパク質輸送におけるユビキチンリガーゼの役割

    2006

    日本応用酵素協会 平成18年度研究助成

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    Authorship:Principal investigator  Grant type:Contract research

  • ペルオキシソーム形成因子群の膜インタフェイス機能と制御

    Grant number:17048020  2005 - 2006

    科学研究費助成事業  特定領域研究

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • ペルオキシソーム形成および細胞機能の制御:ペルオキシン群の分子機能と動態

    Grant number:17028042  2005 - 2006

    科学研究費助成事業  特定領域研究

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • ペルオキシソーム形成因子群複合体の構成と機能制御

    Grant number:17053020  2005

    科学研究費助成事業  特定領域研究

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • ペルオキシソームマトリクスタンパク質輸送機構におけるタンパク質翻訳後修飾の役割

    Grant number:16770101  2004 - 2005

    科学研究費助成事業  若手研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • ペルオキシソームマトリクスタンパク質輸送におけるタンパク質翻訳後修飾の役割

    2004

    花王芸術・科学財団 平成16年度研究助成

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    Authorship:Principal investigator  Grant type:Contract research

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Educational Activities

  • A experimental class of Applied Cell Physiology.
    Classes of General Science Experiments, Molecular Cell Biology, and Research Administration I .

Class subject

  • 分子細胞生物学

    2024.10 - 2025.3   Second semester

  • 応用細胞機能学実験

    2024.4 - 2024.9   First semester

  • 分子細胞生物学

    2023.10 - 2024.3   Second semester

  • 応用細胞機能学実験

    2023.4 - 2023.9   First semester

  • 分子細胞生物学

    2022.10 - 2023.3   Second semester

  • 応用細胞機能学実験

    2022.4 - 2022.9   First semester

  • 分子細胞生物学

    2021.10 - 2022.3   Second semester

  • 自然科学総合実験(生物科学)

    2021.4 - 2021.9   First semester

  • 応用細胞機能学実験

    2021.4 - 2021.9   First semester

  • 応用細胞機能学実験

    2020.10 - 2021.3   Second semester

  • 分子細胞生物学

    2020.10 - 2021.3   Second semester

  • 自然科学総合実験(生物科学)

    2019.10 - 2020.3   Second semester

  • 分子細胞生物学

    2019.10 - 2020.3   Second semester

  • 応用細胞機能学実験

    2019.4 - 2019.9   First semester

  • 自然科学総合実験(生物科学)

    2018.10 - 2019.3   Second semester

  • 応用細胞生物学実験

    2018.4 - 2018.9   First semester

  • 自然科学総合実験(生物科学)

    2017.10 - 2018.3   Second semester

  • 応用細胞生物学実験

    2017.4 - 2017.9   First semester

  • 自然科学総合実験(生物科学)

    2016.10 - 2017.3   Second semester

  • 応用細胞生物学実験

    2016.4 - 2016.9   First semester

  • 自然科学総合実験(生物科学)

    2015.10 - 2016.3   Second semester

  • 応用細胞生物学実験

    2015.4 - 2015.9   First semester

  • 自然科学総合実験(生物科学)

    2014.10 - 2015.3   Second semester

  • 応用細胞生物学実験

    2014.4 - 2014.9   First semester

  • 自然科学総合実験(生物科学)

    2013.4 - 2013.9   First semester

  • 代謝生理学実験

    2012.10 - 2013.3   Second semester

  • 自然科学総合実験(生物科学)

    2012.4 - 2012.9   First semester

  • 代謝生理学実験

    2011.10 - 2012.3   Second semester

  • 自然科学総合実験(生物科学)

    2011.4 - 2011.9   First semester

  • 代謝生理学実験

    2010.10 - 2011.3   Second semester

  • 自然科学総合実験(生物科学)

    2010.4 - 2010.9   First semester

  • 代謝生理学実験

    2009.10 - 2010.3   Second semester

  • 自然科学総合実験(生物科学)

    2009.4 - 2009.9   First semester

  • 代謝生理学実験

    2008.10 - 2009.3   Second semester

  • 自然科学総合実験(生物科学)

    2008.4 - 2008.9   First semester

  • 代謝生理学実験

    2007.10 - 2008.3   Second semester

  • 代謝生理学実験

    2006.10 - 2007.3   Second semester

  • 代謝生理学実験

    2005.10 - 2006.3   Second semester

  • 代謝生理学実験

    2004.10 - 2005.3   Second semester

  • 代謝生理学実験

    2003.10 - 2004.3   Second semester

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FD Participation

  • 2024.3   Role:Participation   Title:有体物管理センターの業務および 成果有体物収入の配分率の変更について

    Organizer:University-wide

  • 2023.3   Role:Participation   Title:【生物学科】大学発明の出願・権利化に関するFD

    Organizer:Undergraduate school department

  • 2022.3   Role:Participation   Title:全学FD:メンタルヘルス講演会

    Organizer:University-wide

  • 2022.3   Role:Participation   Title:入学者選抜試験に関するFD

    Organizer:Undergraduate school department

  • 2020.12   Role:Participation   Title:新型コロナウィルス感染拡大状況での学生のメンタルヘルス

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2019.10   Role:Participation   Title:(生物学科FD)科研費改革後の学術研究動向について

    Organizer:Undergraduate school department

  • 2018.5   Role:Participation   Title:生物学科教育支援室の5年間とこれから

    Organizer:Undergraduate school department

  • 2016.8   Role:Participation   Title:高大連携・大学からのアプローチ

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2014.6   Role:Participation   Title:改定GPA制度の実施のためのFD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2013.3   Role:Participation   Title:理学研究院 化学部門・理学研究院 共同開催 平成24年度FD・HD講演会「アクティブ・ラーニング:入門と実践」

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2008.12   Role:Participation   Title:平成20年度システム生命科学府ファカルティディベロップメント

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2005.3   Role:Participation   Title:平成16年度理学部ファカルティディベロップメント

    Organizer:[Undergraduate school/graduate school/graduate faculty]

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Outline of Social Contribution and International Cooperation activities

  • ESSP (Excellent Students in Science) project for high school students

Social Activities

  • エクセレント・スチューデント・イン・サイエンス育成プロジェクト(ESSP ver.2)の実施(高校生対象)。 試験で選抜した少人数の高校生に対して、5日間の講義、実験を行った。

    2023.8

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    Audience: Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

  • エクセレント・スチューデント・イン・サイエンス育成プロジェクト(ESSP ver.2)の実施(高校生対象)。 試験で選抜した少人数の高校生に対して、5日間の講義、実験を行った。

    2022.8

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    Audience: Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

  • エクセレント・スチューデント・イン・サイエンス育成プロジェクト(ESSP ver.2)の実施(高校生対象)。 試験で選抜した少人数の高校生に対して、5日間の講義、実験を行った。

    2021.8

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    Audience: Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

Travel Abroad

  • 2013.12

    Staying countory name 1:Netherlands   Staying institution name 1:Hampshire Hotel Plaza Groningen

  • 2011.2 - 2011.11

    Staying countory name 1:Singapore   Staying institution name 1:National University of Singapore

  • 2009.11

    Staying countory name 1:United States   Staying institution name 1:Institute for Systems Biology

  • 2003.5

    Staying countory name 1:Germany   Staying institution name 1:リンダウ国際会議場