Updated on 2024/10/07

Information

 

写真a

 
MIURA TAKASHI
 
Organization
Faculty of Medical Sciences Research Center for Human Disease Modeling Professor
Faculty of Medical Sciences Research Center for Human Disease Modeling(Concurrent)
School of Medicine Department of Medicine(Concurrent)
Graduate School of Medical Sciences Department of Medicine(Concurrent)
Graduate School of Medical Sciences Department of Health Care Administration and Management(Concurrent)
Title
Professor
Contact information
メールアドレス
Tel
0926426048
Homepage
External link

Research Areas

  • Life Science / Anatomy

  • Life Science / Developmental biology

  • Natural Science / Basic mathematics

  • Natural Science / Applied mathematics and statistics

  • Life Science / Morphology and anatomical structure

Degree

  • Ph. D

Research History

  • 2000/4/1 京都大学大学院医学研究科   

Research Interests・Research Keywords

  • Research theme: Anatomy

    Keyword: Anatomy

    Research period: 2024

  • Research theme: spontaneous pattern formation

    Keyword: spontaneous pattern formation

    Research period: 2024

  • Research theme: Developmental Biology

    Keyword: Developmental Biology

    Research period: 2024

  • Research theme: Mathematical Biology

    Keyword: Mathematical Biology

    Research period: 2024

  • Research theme: reaction-diffusion system

    Keyword: reaction-diffusion system

    Research period: 2024

  • Research theme: microfluidic device

    Keyword: microfluidic device

    Research period: 2024

  • Research theme: Mathematical modeling of biological pattern formation phenomena and their experimental verification

    Keyword: Pattern formation, mathematical modeling, reaction-diffusion, particle system, developmental biology

    Research period: 1996.4 - 2013.2

Papers

  • Exploiting mechanisms for hierarchical branching structure of lung airway Reviewed

    Hisako Takigawa-Imamura, Katsumi Fumoto, Hiroaki Takesue, Takashi Miura

    PLoS ONE   19 ( 8 )   e0309464   2024.8   ISSN:1932-6203 eISSN:1932-6203

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    Authorship:Last author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:PLoS ONE  

    The lung airways exhibit distinct features with long, wide proximal branches and short, thin distal branches, crucial for optimal respiratory function. In this study, we investigated the mechanism behind this hierarchical structure through experiments and modeling, focusing on the regulation of branch length and width during the pseudoglandular stage. To evaluate the response of mouse lung epithelium to fibroblast growth factor 10 (FGF10), we monitored the activity of extracellular signal-regulated kinase (ERK). ERK activity exhibited an increase dependent on the curvature of the epithelial tissue, which gradually decreased with the progression of development. We then constructed a computational model that incorporates curvature-dependent growth to predict its impact on branch formation. It was demonstrated that branch length is determined by the curvature dependence of growth. Next, in exploring branch width regulation, we considered the effect of apical constriction, a mechanism we had previously proposed to be regulated by Wnt signaling. Analysis of a mathematical model representing apical constriction showed that branch width is determined by cell shape. Finally, we constructed an integrated computational model that includes curvature-dependent growth and cell shape controls, confirming their coordination in regulating branch formation. This study proposed that changes in the autonomous property of the epithelium may be responsible for the progressive branch morphology.

    DOI: 10.1371/journal.pone.0309464

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  • Analyzing the effect of cell rearrangement on Delta-Notch pattern formation Reviewed

    Toshiki Oguma, Hisako Takigawa-Imamura, Tomoyasu Shinoda, Shuntaro Ogura, Akiyoshi Uemura, Takaki Miyata, Philip K. Maini, Takashi Miura

    Physical Review E   107 ( 6 )   2023.6

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    Language:Others   Publishing type:Research paper (scientific journal)  

    DOI: 10.1103/physreve.107.064404

  • Mechanism of interdigitation formation at apical boundary of MDCK cell Reviewed International journal

    Shintaro Miyazaki, Tetsuhisa Otani, Kei Sugihara, Toshihiko Fujimori, Mikio Furuse, Takashi Miura

    iScience   106594 - 106594   2023.4

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    DOI: 10.1016/j.isci.2023.106594

  • Pericytes and shear stress each alter the shape of a self-assembled vascular network Reviewed International journal

    Kazuya Fujimoto, Scott Erickson, Masamune Nakayama, Hroki Ihara, Kei Sugihara, Yuji Nashimoto, Koichi Nishiyama, Takashi Miura, Ryuji Yokokawa

    Lab on a Chip   23 ( 2 )   306 - 317   2023.1   ISSN:1473-0197 eISSN:1473-0189

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Royal Society of Chemistry (RSC)  

    Blood vessel morphology is dictated by mechanical and biochemical cues. Among these, flow-induced shear stress and pericytes have been studied using an on-chip vascular network, for understanding the relationship between...

    DOI: 10.1039/d2lc00605g

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  • Tmsb10 triggers fetal Leydig differentiation by suppressing the RAS/ERK pathway Reviewed International journal

    Miki Inoue, Takashi Baba, Fumiya Takahashi, Miho Terao, Shogo Yanai, Yuichi Shima, Daisuke Saito, Kei Sugihara, Takashi Miura, Shuji Takada, Mikita Suyama, Yasuyuki Ohkawa, Ken-ichirou Morohashi

    Communications Biology   5 ( 1 )   974 - 974   2022.9

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    Abstract

    Leydig cells in fetal testes play crucial roles in masculinizing fetuses through androgen production. Gene knockout studies have revealed that growth factors are implicated in fetal Leydig cell (FLC) differentiation, but little is known about the mechanisms regulating this process. We investigate this issue by characterizing FLC progenitor cells using single-cell RNA sequencing. The sequence datasets suggest that thymosin β10 (Tmsb10) is transiently upregulated in the progenitors. While studying the function of Tmsb10, we reveal that platelet-derived growth factor (PDGF) regulates ciliogenesis through the RAS/ERK and PI3K/AKT pathways, and thereby promotes desert hedgehog (DHH)-dependent FLC differentiation. Tmsb10 expressed in the progenitor cells induces their differentiation into FLCs by suppressing the RAS/ERK pathway. Through characterizing the transiently expressed Tmsb10 in the FLC progenitors, this study unveils the molecular process of FLC differentiation and shows that it is cooperatively induced by DHH and PDGF.

    DOI: 10.1038/s42003-022-03941-5

  • Quantitative modeling of regular retinal microglia distribution Reviewed

    Yoshie Endo, Daisuke Asanuma, Shigeyuki Namiki, Kei Sugihara, Kenzo Hirose, Akiyoshi Uemura, Yoshiaki Kubota, Takashi Miura

    Scientific Reports   11 ( 1 )   2021.12

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    <title>Abstract</title>Microglia are resident immune cells in the central nervous system, showing a regular distribution. Advancing microscopy and image processing techniques have contributed to elucidating microglia’s morphology, dynamics, and distribution. However, the mechanism underlying the regular distribution of microglia remains to be elucidated. First, we quantitatively confirmed the regularity of the distribution pattern of microglial soma in the retina. Second, we formulated a mathematical model that includes factors that may influence regular distribution. Next, we experimentally quantified the model parameters (cell movement, process formation, and ATP dynamics). The resulting model simulation from the measured parameters showed that direct cell–cell contact is most important in generating regular cell spacing. Finally, we tried to specify the molecular pathway responsible for the repulsion between neighboring microglia.

    DOI: 10.1038/s41598-021-01820-3

  • Mathematical Modeling of Dynamic Cellular Association Patterns in Seminiferous Tubules Reviewed International journal

    Mari Kawamura, Kei Sugihara, Hisako Takigawa-Imamura, Toshiyuki Ogawa, Takashi Miura

    Bulletin of Mathematical Biology   83 ( 4 )   33 - 33   2021.4

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    In vertebrates, sperm is generated in testicular tube-like structures called seminiferous tubules. The differentiation stages of spermatogenesis exhibit a dynamic spatiotemporal wavetrain pattern. There are two types of pattern-the vertical type, which is observed in mice, and the helical type, which is observed in humans. The mechanisms of this pattern difference remain little understood. In the present study, we used a three-species reaction-diffusion model to reproduce the wavetrain pattern observed in vivo. We hypothesized that the wavelength of the pattern in mice was larger than that in humans and undertook numerical simulations. We found complex patterns of helical and vertical pattern frequency, which can be understood by pattern selection using boundary conditions. From these theoretical results, we predicted that a small number of vertical patterns should be present in human seminiferous tubules. We then found vertical patterns in histological sections of human tubules, consistent with the theoretical prediction. Finally, we showed that the previously reported irregularity of the human pattern could be reproduced using two factors: a wider unstable wavenumber range and the irregular geometry of human compared with mouse seminiferous tubules. These results show that mathematical modeling is useful for understanding the pattern dynamics of seminiferous tubules in vivo.

    DOI: 10.1007/s11538-021-00863-x

  • A new perfusion culture method with a self-organized capillary network Reviewed International journal

    Kei Sugihara, Yoshimi Yamaguchi, Shiori Usui, Yuji Nashimoto, Sanshiro Hanada, Etsuko Kiyokawa, Akiyoshi Uemura, Ryuji Yokokawa, Koichi Nishiyama, Takashi Miura

    PLOS ONE   15 ( 10 )   e0240552 - e0240552   2020.10

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    A lack of perfusion has been one of the most significant obstacles for three-dimensional culture systems of organoids and embryonic tissues. Here, we developed a simple and reliable method to implement a perfusable capillary network in vitro. The method employed the self-organization of endothelial cells to generate a capillary network and a static pressure difference for culture medium circulation, which can be easily introduced to standard biological laboratories and enables long-term cultivation of vascular structures. Using this culture system, we perfused the lumen of the self-organized capillary network and observed a flow-induced vascular remodeling process, cell shape changes, and collective cell migration. We also observed an increase in cell proliferation around the self-organized vasculature induced by flow, indicating functional perfusion of the culture medium. We also reconstructed extravasation of tumor and inflammatory cells, and circulation inside spheroids including endothelial cells and human lung fibroblasts. In conclusion, this system is a promising tool to elucidate the mechanisms of various biological processes related to vascular flow.

    DOI: 10.1371/journal.pone.0240552

  • Mechanisms of endothelial cell coverage by pericytes: computational modelling of cell wrapping and in vitro experiments Reviewed International journal

    Kei Sugihara, Saori Sasaki, Akiyoshi Uemura, Satoru Kidoaki, Takashi Miura

    Journal of The Royal Society Interface   17 ( 162 )   20190739 - 20190739   2020.1

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    Pericytes (PCs) wrap around endothelial cells (ECs) and perform diverse functions in physiological and pathological processes. Although molecular interactions between ECs and PCs have been extensively studied, the morphological processes at the cellular level and their underlying mechanisms have remained elusive. In this study, using a simple cellular Potts model, we explored the mechanisms for EC wrapping by PCs. Based on the observed
    <italic>in vitro</italic>
    cell wrapping in three-dimensional PC–EC coculture, the model identified four putative contributing factors: preferential adhesion of PCs to the extracellular matrix (ECM), strong cell–cell adhesion, PC surface softness and larger PC size. While cell–cell adhesion can contribute to the prevention of cell segregation and the degree of cell wrapping, it cannot determine the orientation of cell wrapping alone. While atomic force microscopy revealed that PCs have a larger Young’s modulus than ECs, the experimental analyses supported preferential ECM adhesion and size asymmetry. We also formulated the corresponding energy minimization problem and numerically solved this problem for specific cases. These results give biological insights into the role of PC–ECM adhesion in PC coverage. The modelling framework presented here should also be applicable to other cell wrapping phenomena observed
    <italic>in vivo</italic>
    .

    DOI: 10.1098/rsif.2019.0739

  • Integrating perfusable vascular networks with a three-dimensional tissue in a microfluidic device. Reviewed International journal

    Yuji Nashimoto, Tomoya Hayashi, Itsuki Kunita, Akiko Nakamasu, Yu-Suke Torisawa, Masamune Nakayama, Hisako Takigawa-Imamura, Hidetoshi Kotera, Koichi Nishiyama, Takashi Miura, Ryuji Yokokawa

    Integrative biology : quantitative biosciences from nano to macro   9 ( 6 )   506 - 518   2017.6

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    Creating vascular networks in tissues is crucial for tissue engineering. Although recent studies have demonstrated the formation of vessel-like structures in a tissue model, long-term culture is still challenging due to the lack of active perfusion in vascular networks. Here, we present a method to create a three-dimensional cellular spheroid with a perfusable vascular network in a microfluidic device. By the definition of the cellular interaction between human lung fibroblasts (hLFs) in a spheroid and human umbilical vein endothelial cells (HUVECs) in microchannels, angiogenic sprouts were induced from microchannels toward the spheroid; the sprouts reached the vessel-like structures in a spheroid to form a continuous lumen. We demonstrated that the vascular network could administer biological substances to the interior of the spheroid. As cell density in the spheroid is similar to that of a tissue, the perfusable vasculature model opens up new possibilities for a long-term tissue culture in vitro.

    DOI: 10.1039/c7ib00024c

  • Exogenous Cellulase Switches Cell Interdigitation to Cell Elongation in an RIC1-dependent Manner in Arabidopsis thaliana Cotyledon Pavement Cells Reviewed

    Takumi Higaki, Hisako Takigawa-Imamura, Kae Akita, Natsumaro Kutsuna, Ryo Kobayashi, Seiichiro Hasezawa, Takashi Miura

    Plant and Cell Physiology   pcw183 - pcw183   2016.12

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    DOI: 10.1093/pcp/pcw183

  • A Theoretical Model of Jigsaw-Puzzle Pattern Formation by Plant Leaf Epidermal Cells Reviewed

    Takumi Higaki, Natsumaro Kutsuna, Kae Akita, Hisako Takigawa-Imamura, Kenji Yoshimura, Takashi Miura

    PLOS COMPUTATIONAL BIOLOGY   12 ( 4 )   e1004833   2016.4

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    Plant leaf epidermal cells exhibit a jigsaw puzzle-like pattern that is generated by interdigitation of the cell wall during leaf development. The contribution of two ROP GTPases, ROP2 and ROP6, to the cytoskeletal dynamics that regulate epidermal cell wall interdigitation has already been examined; however, how interactions between these molecules result in pattern formation remains to be elucidated. Here, we propose a simple interface equation model that incorporates both the cell wall remodeling activity of ROP GTPases and the diffusible signaling molecules by which they are regulated. This model successfully reproduces pattern formation observed in vivo, and explains the counterintuitive experimental results of decreased cellulose production and increased thickness. Our model also reproduces the dynamics of three-way cell wall junctions. Therefore, this model provides a possible mechanism for cell wall interdigitation formation in vivo.

    DOI: 10.1371/journal.pcbi.1004833

  • Dynamics of VEGF matrix-retention in vascular network patterning Reviewed

    Alvaro Köhn-Luque, Walter de Back, Yoshimi Yamaguchi, Kenji Yoshimura, Miguel A Herrero, Takashi Miura

    Physical Biology   10 ( 6 )   066007 - 066007   2013.12

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    DOI: 10.1088/1478-3975/10/6/066007

  • Reaction-Diffusion Model as a Framework for Understanding Biological Pattern Formation Reviewed

    Shigeru Kondo, Takashi Miura

    Science   329 ( 5999 )   1616 - 1620   2010.9

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    DOI: 10.1126/science.1179047

  • Mechanism of skull suture maintenance and interdigitation Reviewed

    Takashi Miura, Chad A. Perlyn, Masato Kinboshi, Naomichi Ogihara, Mikiko Kobayashi-Miura, Gillian M. Morriss-Kay, Kohei Shiota

    Journal of Anatomy   215 ( 6 )   642 - 655   2009.12

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    DOI: 10.1111/j.1469-7580.2009.01148.x

  • The cyst-branch difference in developing chick lung results from a different morphogen diffusion coefficient Reviewed

    Miura, T., Hartmann, D., Kinboshi, M., Komada, M., Ishibashi, M., Shiota, K.

    Mechanisms of Development   126 ( 3-4 )   160 - 172   2009.4

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    The developing avian lung is formed mainly by branching morphogenesis, but there is also a unique cystic structure, the air sac, in the ventral region. It has been shown that mesenchymal tissue is responsible for the differential development of a cystic or branched structure, and that the transcription factor Hoxb may be involved in determining this regional difference. We have previously developed two scenarios for branch-cyst transition, both experimentally and theoretically: increased production or increased diffusion of FGF. The aim of the present study was to discover whether one of these scenarios actually operates in the ventral region of the chick lung. We found that the FGF10 level was lower while the diffusion of FGF10 was more rapid in the ventral lung, indicating that the second scenario is more plausible. There are two possibilities as to why the diffusion of FGF10 differs between the two regions: (1) diffusion is facilitated by the looser tissue organisation of the ventral lung mesenchyme; (2) stronger expression of heparan sulphate proteoglycan ( HSPG) in the dorsal lung traps FGF and decreases the effective diffusion coefficient. Mathematical analysis showed that the dorsal-ventral difference in the amount of HSPG is not sufficient to generate the observed difference in pattern, indicating that both extracellular matrix and tissue architecture play a role in this system. These results suggest that the regional cystic-branched difference within the developing chick lung results from a difference in the rate of diffusion of morphogen between the ventral and dorsal regions due to differential levels of HSPG and a different mesenchymal structure. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

    DOI: 10.1016/j.mod.2008.11.006

  • Modelling in vitro lung branching morphogenesis during development Reviewed

    Dirk Hartmann, Takashi Miura

    Journal of Theoretical Biology   242 ( 4 )   862 - 872   2006.10

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    DOI: 10.1016/j.jtbi.2006.05.009

  • Mixed-mode pattern in Doublefoot mutant mouse limb—Turing reaction–diffusion model on a growing domain during limb development Reviewed

    Takashi Miura, Kohei Shiota, Gillian Morriss-Kay, Philip K. Maini

    Journal of Theoretical Biology   240 ( 4 )   562 - 573   2006.6

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    DOI: 10.1016/j.jtbi.2005.10.016

  • Speed of pattern appearance in reaction-diffusion models: implications in the pattern formation of limb bud mesenchyme cells Reviewed

    Takashi Miura, Philip K. Maini

    Bulletin of Mathematical Biology   66 ( 4 )   627 - 649   2004.7

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    DOI: 10.1016/j.bulm.2003.09.009

  • Depletion of FGF acts as a lateral inhibitory factor in lung branching morphogenesis in vitro Reviewed

    Takashi Miura, Kohei Shiota

    Mechanisms of Development   116 ( 1-2 )   29 - 38   2002.8

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    DOI: 10.1016/s0925-4773(02)00132-6

  • TGFβ2 acts as an 'activator' molecule in reaction-diffusion model and is involved in cell sorting phenomenon in mouse limb micromass culture Reviewed

    Takashi Miura, Kohei Shiota

    Developmental Dynamics   217 ( 3 )   241 - 249   2000.4

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    It was previously speculated that TGFβ acts as an 'activator'-molecule in chondrogenic pattern formation in the limb micromass culture system, but its precise role and relationship with the cell sorting phenomenon have not been properly studied. In the present study, we examined whether the TGFβ2 molecule satisfies the necessary conditions for an 'activator'-molecule in the reaction-diffusion model. Firstly, we showed that TGFβ2 became localized at chondrogenic sites during the establishment of a chondrogenic pattern, and exogenous TGFβ2 promoted chondrogenesis when added in the culture medium. Secondly, TGFβ2 protein was shown to promote the production of its own mRNA after 3 hr, indicating that a positive feedback mechanism exists which may be responsible for the emergence of the chondrogenic pattern. We then found that when locally applied with beads, TGFβ2 suppressed chondrogenesis around the beads, indicating it induces the lateral inhibitory mechanism, which is a key element for the formation of the periodic pattern. We also examined the possible effects of TGFβ2 on the cell sorting phenomenon and found that TGFβ2 exerts differential chemotactic activity on proximal and distal mesenchyme cells of the limb bud, and at very early phases of differentiation TGFβ2 promotes the expression of N-cadherin protein which is known to be involved in pattern formation in this culture system. These findings suggest that TGFβ2 acts as an 'activator'-like molecule in chondrogenic pattern formation in vitro, and is possibly responsible for the cell sorting phenomenon. (C) 2000 Wiley- Liss, Inc.

    DOI: 10.1002/(SICI)1097-0177(200003)217:3<241::AID-DVDY2>3.0.CO;2-K

  • Cholesterol synthesis is essential for the growth of liver metastasis-prone colorectal cancer cells. Reviewed International journal

    Kumiko Taniguchi, Kei Sugihara, Takashi Miura, Daisuke Hoshi, Susumu Kohno, Chiaki Takahashi, Eishu Hirata, Etsuko Kiyokawa

    Cancer science   2024.9   ISSN:1347-9032 eISSN:1349-7006

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    Metastasis to the liver is a leading cause of death in patients with colorectal cancer. To investigate the characteristics of cancer cells prone to metastasis, we utilized an isogenic model of BALB/c and colon tumor 26 (C26) cells carrying an active KRAS mutation. Liver metastatic (LM) 1 cells were isolated from mice following intrasplenic transplantation of C26 cells. Subsequent injections of LM1 cells generated LM2 cells, and after four cycles, LM4 cells were obtained. In vitro, using a perfusable capillary network system, we found comparable extravasation frequencies between C26 and LM4 cells. Both cell lines showed similar growth rates in vitro. However, C26 cells showed higher glucose consumption, whereas LM4 cells incorporated more fluorescent fatty acids (FAs). Biochemical analysis revealed that LM4 cells had higher cholesterol levels than C26 cells. A correlation was observed between fluorescent FAs and cholesterol levels detected using filipin III. LM4 cells utilized FAs as a source for cholesterol synthesis through acetyl-CoA metabolism. In cellular analysis, cholesterol accumulated in punctate regions, and upregulation of NLRP3 and STING proteins, but not mTOR, was observed in LM4 cells. Treatment with a cholesterol synthesis inhibitor (statin) induced LM4 cell death in vitro and suppressed LM4 cell growth in the livers of nude mice. These findings indicate that colorectal cancer cells prone to liver metastasis show cholesterol-dependent growth and that statin therapy could help treat liver metastasis in immunocompromised patients.

    DOI: 10.1111/cas.16331

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  • Stripe and spot selection in cusp patterning of mammalian molar formation Reviewed

    Wataru Morita, Naoki Morimoto, Keishi Otsu, Takashi Miura

    Scientific Reports   12 ( 1 )   2022.12

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    Abstract

    Tooth development is governed largely by epithelial–mesenchymal interactions and is mediated by numerous signaling pathways. This type of morphogenetic processes has been explained by reaction–diffusion systems, especially in the framework of a Turing model. Here we focus on morphological and developmental differences between upper and lower molars in mice by modeling 2D pattern formation in a Turing system. Stripe vs. spot patterns are the primary types of variation in a Turing model. We show that the complexity of the cusp cross-sections can distinguish between stripe vs. spot patterns, and mice have stripe-like upper and spot-like lower molar morphologies. Additionally, our computational modeling that incorporates empirical data on tooth germ growth traces the order of cusp formation and relative position of the cusps in upper and lower molars in mice. We further propose a hypothetical framework of developmental mechanism that could help us understand the evolution of the highly variable nature of mammalian molars associated with the acquisition of the hypocone and the increase of lophedness.

    DOI: 10.1038/s41598-022-13539-w

  • Electrochemical sensing of oxygen metabolism for a three-dimensional cultured model with biomimetic vascular flow Reviewed

    Yuji Nashimoto, Rei Mukomoto, Takuto Imaizumi, Takato Terai, Shotaro Shishido, Kosuke Ino, Ryuji Yokokawa, Takashi Miura, Kunishige Onuma, Masahiro Inoue, Hitoshi Shiku

    Biosensors and Bioelectronics   114808 - 114808   2022.10

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    DOI: 10.1016/j.bios.2022.114808

  • Mechanical loading of intraluminal pressure mediates wound angiogenesis by regulating the TOCA family of F-BAR proteins. Reviewed International journal

    Shinya Yuge, Koichi Nishiyama, Yuichiro Arima, Yasuyuki Hanada, Eri Oguri-Nakamura, Sanshiro Hanada, Tomohiro Ishii, Yuki Wakayama, Urara Hasegawa, Kazuya Tsujita, Ryuji Yokokawa, Takashi Miura, Toshiki Itoh, Kenichi Tsujita, Naoki Mochizuki, Shigetomo Fukuhara

    Nature communications   13 ( 1 )   2594 - 2594   2022.5

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    Angiogenesis is regulated in coordinated fashion by chemical and mechanical cues acting on endothelial cells (ECs). However, the mechanobiological mechanisms of angiogenesis remain unknown. Herein, we demonstrate a crucial role of blood flow-driven intraluminal pressure (IP) in regulating wound angiogenesis. During wound angiogenesis, blood flow-driven IP loading inhibits elongation of injured blood vessels located at sites upstream from blood flow, while downstream injured vessels actively elongate. In downstream injured vessels, F-BAR proteins, TOCA1 and CIP4, localize at leading edge of ECs to promote N-WASP-dependent Arp2/3 complex-mediated actin polymerization and front-rear polarization for vessel elongation. In contrast, IP loading expands upstream injured vessels and stretches ECs, preventing leading edge localization of TOCA1 and CIP4 to inhibit directed EC migration and vessel elongation. These data indicate that the TOCA family of F-BAR proteins are key actin regulatory proteins required for directed EC migration and sense mechanical cell stretching to regulate wound angiogenesis.

    DOI: 10.1038/s41467-022-30197-8

  • Mathematical modeling of palatal suture pattern formation: morphological differences between sagittal and palatal sutures Reviewed

    Nobuhide Shibusawa, Yoshie Endo, Naoki Morimoto, Ichiro Takahashi, Takashi Miura

    Scientific Reports   11 ( 1 )   2021.12

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    <title>Abstract</title>The median palatal suture serves as a growth center for the maxilla; inadequate growth at this site causes malocclusion and dental crowding. However, the pattern formation mechanism of palatal sutures is poorly understood compared with that of calvarial sutures such as the sagittal suture. In the present study, therefore, we compared the morphological characteristics of sagittal and palatal sutures in human bone specimens. We found that palatal suture width was narrower than sagittal suture width, and the interdigitation amplitude of the palatal suture was lower than that of the sagittal suture. These tendencies were also observed in the neonatal stage. However, such differences were not observed in other animals such as chimpanzees and mice. We also used a mathematical model to reproduce the differences between palatal and sagittal sutures. After an extensive parameter search, we found two conditions that could generate the difference in interdigitation amplitude and suture width: bone differentiation threshold <inline-formula><alternatives><tex-math>$$v_c$$</tex-math><mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML">
    <mml:msub>
    <mml:mi>v</mml:mi>
    <mml:mi>c</mml:mi>
    </mml:msub>
    </mml:math></alternatives></inline-formula> and growth speed <italic>c</italic>. We discuss possible biological interpretations of the observed pattern difference and its cause.

    DOI: 10.1038/s41598-021-88255-y

  • Effective nonlocal kernels on reaction–diffusion networks Reviewed

    Shin-Ichiro Ei, Hiroshi Ishii, Shigeru Kondo, Takashi Miura, Yoshitaro Tanaka

    Journal of Theoretical Biology   509   110496 - 110496   2021.1

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    DOI: 10.1016/j.jtbi.2020.110496

  • Mesenchymal glioblastoma-induced mature de-novo vessel formation of vascular endothelial cells in a microfluidic device Reviewed

    Takeo Amemiya, Nobuhiro Hata, Masahiro Mizoguchi, Ryuji Yokokawa, Yoichiro Kawamura, Ryusuke Hatae, Yuhei Sangatsuda, Daisuke Kuga, Yutaka Fujioka, Kosuke Takigawa, Yojiro Akagi, Koji Yoshimoto, Koji Iihara, Takashi Miura

    Molecular Biology Reports   2021.1

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    <title>Abstract</title>High vascularization is a biological characteristic of glioblastoma (GBM); however, an <italic>in-vitro</italic> experimental model to verify the mechanism and physiological role of vasculogenesis in GBM is not well-established. Recently, we established a self-organizing vasculogenic model using human umbilical vein endothelial cells (HUVECs) co-cultivated with human lung fibroblasts (hLFs). Here, we exploited this system to establish a realistic model of vasculogenesis in GBM. We developed two polydimethylsiloxane (PDMS) devices, a doughnut-hole dish and a 5-lane microfluidic device to observe the contact-independent effects of glioblastoma cells on HUVECs. We tested five patient-derived and five widely used GBM cell lines. Confocal fluorescence microscopy was used to observe the morphological changes in Red Fluorescent Protein (RFP)-HUVECs and fluorescein isothiocyanate (FITC)-dextran perfusion. The genetic and expression properties of GBM cell lines were analyzed. The doughnut-hole dish assay revealed KNS1451 as the only cells to induce HUVEC transformation to vessel-like structures, similar to hLFs. The 5-lane device assay demonstrated that KNS1451 promoted the formation of a vascular network that was fully perfused, revealing the functioning luminal construction. Microarray analysis revealed that KNS1451 is a mesenchymal subtype of GBM. Using a patient-derived mesenchymal GBM cell line, mature <italic>de-novo</italic> vessel formation could be induced in HUVECs by contact-independent co-culture with GBM in a microfluidic device. These results support the development of a novel in vitro research model and provide novel insights in the neovasculogenic mechanism of GBM and may potentially facilitate the future detection of unknown molecular targets.

    DOI: 10.1007/s11033-020-06061-7

  • Noise-induced scaling in skull suture interdigitation Reviewed

    Yuto Naroda, Yoshie Endo, Kenji Yoshimura, Hiroshi Ishii, Shin-Ichiro Ei, Takashi Miura

    PLOS ONE   15 ( 12 )   e0235802 - e0235802   2020.12

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    Sutures, the thin, soft tissue between skull bones, serve as the major craniofacial growth centers during postnatal development. In a newborn skull, the sutures are straight; however, as the skull develops, the sutures wind dynamically to form an interdigitation pattern. Moreover, the final winding pattern had been shown to have fractal characteristics. Although various molecules involved in suture development have been identified, the mechanism underlying the pattern formation remains unknown. In a previous study, we reproduced the formation of the interdigitation pattern in a mathematical model combining an interface equation and a convolution kernel. However, the generated pattern had a specific characteristic length, and the model was unable to produce a fractal structure with the model. In the present study, we focused on the anterior part of the sagittal suture and formulated a new mathematical model with time–space-dependent noise that was able to generate the fractal structure. We reduced our previous model to represent the linear dynamics of the centerline of the suture tissue and included a time–space-dependent noise term. We showed theoretically that the final pattern from the model follows a scaling law due to the scaling of the dispersion relation in the full model, which we confirmed numerically. Furthermore, we observed experimentally that stochastic fluctuation of the osteogenic signal exists in the developing skull, and found that actual suture patterns followed a scaling law similar to that of the theoretical prediction.

    DOI: 10.1371/journal.pone.0235802

  • Mechanism underlying dynamic scaling properties observed in the contour of spreading epithelial monolayer Reviewed International journal

    Toshiki Oguma, Hisako Takigawa-Imamura, Takashi Miura

    Physical Review E   102 ( 6 )   062408 - 062408   2020.12

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    We found evidence of dynamic scaling in the spreading of Madin-Darby canine kidney (MDCK) cell monolayer, which can be characterized by the Hurst exponent α=0.86 and the growth exponent β=0.73, and theoretically and experimentally clarified the mechanism that governs the contour shape dynamics. Dynamic scaling refers to the roughness of the surface scales, both spatially and temporally. During the spreading of the monolayer, it is known that so-called leader cells generate the driving force and lead the other cells. Our time-lapse observations of cell behavior showed that these leader cells appeared at the early stage of the spreading and formed the monolayer protrusion. Informed by these observations, we developed a simple mathematical model that included differences in cell motility, cell-cell adhesion, and random cell movement. The model reproduced the quantitative characteristics obtained from the experiment, such as the spreading speed, the distribution of the increment, and the dynamic scaling law. Analysis of the model equation shows that the model can reproduce different scaling laws from (α=0.5,β=0.25) to (α=0.9,β=0.75), where the exponents α and β are determined by two dimensionless quantities determined by the microscopic cell behavior. From the analytical result, parameter estimation from the experimental results was achieved. The monolayer on the collagen-coated dishes showed a different scaling law, α=0.74,β=0.68, suggesting that cell motility increased ninefold. This result was consistent with the assay of the single-cell motility. Our study demonstrated that the dynamics of the contour of the monolayer were explained by the simple model, and we propose a mechanism that exhibits the dynamic scaling property.

    DOI: 10.1103/physreve.102.062408

  • Remodeling mechanisms determine size distributions in developing retinal vasculature Reviewed International journal

    Osamu Iizuka, Shotaro Kawamura, Atsushi Tero, Akiyoshi Uemura, Takashi Miura

    PLOS ONE   15 ( 10 )   e0235373 - e0235373   2020.10

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    The development of retinal blood vessels has extensively been used as a model to study vascular pattern formation. To date, various quantitative measurements, such as size distribution have been performed, but the relationship between pattern formation mechanisms and these measurements remains unclear. In the present study, we first focus on the islands (small regions subdivided by the capillary network). We quantitatively measured the island size distribution in the retinal vascular network and found that it tended to exhibit an exponential distribution. We were able to recapitulate this distribution pattern in a theoretical model by implementing the stochastic disappearance of vessel segments around arteries could reproduce the observed exponential distribution of islands. Second, we observed that the diameter distribution of the retinal artery segment obeyed a power law. We theoretically showed that an equal bifurcation branch pattern and Murray's law could reproduce this pattern. This study demonstrates the utility of examining size distribution for understanding the mechanisms of vascular pattern formation.

    DOI: 10.1371/journal.pone.0235373

  • Oxygen consumption rate of tumour spheroids during necrotic-like core formation. Reviewed International journal

    Rei Mukomoto, Yuji Nashimoto, Takato Terai, Takuto Imaizumi, Kaoru Hiramoto, Kosuke Ino, Ryuji Yokokawa, Takashi Miura, Hitoshi Shiku

    The Analyst   2020.7

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    Hypoxia is one of the major hallmarks of solid tumours and is associated with the poor prognosis of various cancers. A multicellular aggregate, termed a spheroid, has been used as a tumour model with a necrotic-like core for more than 45 years. Oxygen metabolism in spheroids has been studied using phosphorescence quenching and oxygen-sensitive electrodes. However, these conventional methods require chemical labelling and physical insertion of the electrode into each spheroid, which may be functionally and structurally disruptive. Scanning electrochemical microscopy (SECM) can non-invasively analyse oxygen metabolism. Here, we used SECM to investigate whether the changes of the internal structure of spheroids affect the oxygen metabolism. We investigated the oxygen consumption rate (OCR) of MCF-7 breast tumour spheroids with and without a necrotic-like core. A numerical simulation was used to describe a method for estimating the OCR of spheroids that settled at the bottom of the conventional culture plates. The OCR per spheroid volume decreased with increasing spheroid radius, indicating the limitation of the oxygen supply to the core of the MCF-7 spheroid. Formation of the necrotic-like core did not affect the oxygen metabolism significantly, implying that the core had minimal contribution to the OCR even before necrosis occurred. OCR analysis using SECM non-invasively monitors the change of oxygen metabolism in tumour spheroids. The approach is promising to evaluate various three-dimensional culture models.

    DOI: 10.1039/d0an00979b

  • A new microcirculation culture method with a self-organized capillary network Reviewed

    Kei Sugihara, Yoshimi Yamaguchi, Shiori Usui, Yuji Nashimoto, Sanshiro Hanada, Etsuko Kiyokawa, Akiyoshi Uemura, Ryuji Yokokawa, Koichi Nishiyama, Takashi Miura

    bioRxiv   2020.5

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    <title>Abstract</title>A lack of microcirculation has been one of the most significant obstacles for three-dimensional culture systems of organoids and embryonic tissues. Here, we developed a simple and reliable method to implement a perfusable capillary network in vitro. The method employed the self-organization of endothelial cells to generate a capillary network and a static pressure difference for culture medium circulation, which can be easily introduced to standard biological laboratories and enables long-term cultivation of vascular structures. Using this culture system, we perfused the lumen of the self-organized capillary network and observed a flow-induced vascular remodeling process, cell shape changes, and collective cell migration. We also observed an increase in cell proliferation around the synthetic vasculature induced by flow, indicating functional perfusion of the culture medium. We also reconstructed extravasation of tumor and inflammatory cells, and circulation inside spheroids including endothelial cells and human lung fibroblasts. In conclusion, this system is a promising tool to elucidate the mechanisms of various biological processes related to vascular flow.

    DOI: 10.1101/2020.05.12.067165

  • Vascularized cancer on a chip: The effect of perfusion on growth and drug delivery of tumor spheroid Reviewed International journal

    Yuji Nashimoto, Ryu Okada, Sanshiro Hanada, Yuichiro Arima, Koichi Nishiyama, Takashi Miura, Ryuji Yokokawa

    Biomaterials   229   119547 - 119547   2020.1

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    Tumor vasculature creates a hostile tumor microenvironment (TME) in vivo and nourishes cancers, resulting in cancer progression and drug resistance. To mimic the biochemical and biomechanical environments of tumors in vitro, several models integrated with a vascular network have been reported. However, the tumor responses to biochemical and biomechanical stimuli were evaluated under static conditions and failed to incorporate the effects of blood flow to tumors. In this study, we present a tumor-on-a-chip platform that enables the evaluation of tumor activities with intraluminal flow in an engineered tumor vascular network. The fibroblasts in the tumor spheroid induced angiogenic sprouts, which constructed a perfusable vascular network in a tumor spheroid. The perfusability of the engineered vascular network was preserved during the culture. Moreover, perfusion for over 24 h significantly increased the proliferation activities of tumor cells and decreased cell death in the spheroid. Drug administration under perfusion condition did not show the dose-dependent effects of anticancer drugs on tumor activities in contrast to the results under static conditions. Our results demonstrate the importance of flow in a vascular network for the evaluation of tumor activities in a drug screening platform.

    DOI: 10.1016/j.biomaterials.2019.119547

  • Claudins and JAM-A coordinately regulate tight junction formation and epithelial polarity Reviewed International journal

    Tetsuhisa Otani, Thanh Phuong Nguyen, Shinsaku Tokuda, Kei Sugihara, Taichi Sugawara, Kyoko Furuse, Takashi Miura, Klaus Ebnet, Mikio Furuse

    Journal of Cell Biology   218 ( 10 )   3372 - 3396   2019.10

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    Tight junctions (TJs) establish the epithelial barrier and are thought to form a membrane fence to regulate epithelial polarity, although the roles of TJs in epithelial polarity remain controversial. Claudins constitute TJ strands in conjunction with the cytoplasmic scaffolds ZO-1 and ZO-2 and play pivotal roles in epithelial barrier formation. However, how claudins and other TJ membrane proteins cooperate to organize TJs remains unclear. Here, we systematically knocked out TJ components by genome editing and show that while ZO-1/ZO-2–deficient cells lacked TJ structures and epithelial barriers, claudin-deficient cells lacked TJ strands and an electrolyte permeability barrier but formed membrane appositions and a macromolecule permeability barrier. Moreover, epithelial polarity was disorganized in ZO-1/ZO-2–deficient cells, but not in claudin-deficient cells. Simultaneous deletion of claudins and a TJ membrane protein JAM-A resulted in a loss of membrane appositions and a macromolecule permeability barrier and in sporadic epithelial polarity defects. These results demonstrate that claudins and JAM-A coordinately regulate TJ formation and epithelial polarity.

    DOI: 10.1083/jcb.201812157

  • Combining Turing and 3D vertex models reproduces autonomous multicellular morphogenesis with undulation, tubulation, and branching Reviewed

    Satoru Okuda, Takashi Miura, Yasuhiro Inoue, Taiji Adachi, Mototsugu Eiraku

    SCIENTIFIC REPORTS   8 ( 1 )   2386   2018.6

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    DOI: 10.1038/s41598-018-24858-2

  • MUTE Directly Orchestrates Cell-State Switch and the Single Symmetric Division to Create Stomata Reviewed

    Soon-Ki Han, Xingyun Qi, Kei Sugihara, Jonathan H. Dang, Takaho A. Endo, Kristen L. Miller, Eun-Deok Kim, Takashi Miura, Keiko U. Torii

    Developmental Cell   45 ( 3 )   303 - 315.e5   2018.5

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    DOI: 10.1016/j.devcel.2018.04.010

  • Elasticity-based boosting of neuroepithelial nucleokinesis via indirect energy transfer from mother to daughter Reviewed

    Tomoyasu Shinoda, Arata Nagasaka, Yasuhiro Inoue, Ryo Higuchi, Yoshiaki Minami, Kagayaki Kato, Makoto Suzuki, Takefumi Kondo, Takumi Kawaue, Kanako Saito, Naoto Ueno, Yugo Fukazawa, Masaharu Nagayama, Takashi Miura, Taiji Adachi, Takaki Miyata

    PLOS BIOLOGY   16 ( 4 )   2018.4

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    Neural progenitor cells (NPCs), which are apicobasally elongated and densely packed in the developing brain, systematically move their nuclei/somata in a cell cycle-dependent manner, called interkinetic nuclear migration (IKNM): apical during G2 and basal during G1. Although intracellular molecular mechanisms of individual IKNM have been explored, how heterogeneous IKNMs are collectively coordinated is unknown. Our quantitative cell-biological and in silico analyses revealed that tissue elasticity mechanically assists an initial step of basalward IKNM. When the soma of an M-phase progenitor cell rounds up using actomyosin within the subapical space, a microzone within 10 mu m from the surface, which is compressed and elastic because of the apical surface's contractility, laterally pushes the densely neighboring processes of non-M-phase cells. The pressed processes then recoil centripetally and basally to propel the nuclei/somata of the progenitor's daughter cells. Thus, indirect neighbor-assisted transfer of mechanical energy from mother to daughter helps efficient brain development.

    DOI: 10.1371/journal.pbio.2004426

  • 組織の形態形成解明に資するマイクロ流体デバイス内での血管網の構築とその応用 Reviewed

    梨本 裕司, 寺岡 佑佳子, 有馬 勇一郎, 花田 三四郎, 中益 朗子, 小寺 秀俊, 西山 功一, 三浦 岳, 横川 隆司

    電学論E   138 ( 7 )   275 - 280   2018.4

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    Engineering a Perfusable Vascular Network in a Microfluidic Device for a Morphological Analysis

    DOI: 10.1541/ieejsmas.138.275

  • Perfusable Vascular Network with a Tissue Model in a Microfluidic Device Reviewed International journal

    Yuji Nashimoto, Yukako Teraoka, Ramin Banan Sadeghian, Akiko Nakamasu, Yuichiro Arima, Sanshiro Hanada, Hidetoshi Kotera, Koichi Nishiyama, Takashi Miura, Ryuji Yokokawa

    Journal of Visualized Experiments   ( 134 )   2018.4

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    A spheroid (a multicellular aggregate) is regarded as a good model of living tissues in the human body. Despite the significant advancement in the spheroid cultures, a perfusable vascular network in the spheroids remains a critical challenge for long-term culture required to maintain and develop their functions, such as protein expressions and morphogenesis. The protocol presents a novel method to integrate a perfusable vascular network within the spheroid in a microfluidic device. To induce a perfusable vascular network in the spheroid, angiogenic sprouts connected to microchannels were guided to the spheroid by utilizing angiogenic factors from human lung fibroblasts cultured in the spheroid. The angiogenic sprouts reached the spheroid, merged with the endothelial cells co-cultured in the spheroid, and formed a continuous vascular network. The vascular network could perfuse the interior of the spheroid without any leakage. The constructed vascular network may be further used as a route for supply of nutrients and removal of waste products, mimicking blood circulation in vivo. The method provides a new platform in spheroid culture toward better recapitulation of living tissues.

    DOI: 10.3791/57242

  • Mathematical modeling for meshwork formation of endothelial cells in fibrin gels Reviewed

    Daiki Sasaki, Hitomi Nakajima, Yoshimi Yamaguchi, Ryuji Yokokawa, Shin-Ichiro Ei, Takashi Miura

    Journal of Theoretical Biology   429   95 - 104   2017.9

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    DOI: 10.1016/j.jtbi.2017.06.012

  • ENGINEERING A THREE-DIMENSIONAL TISSUE MODEL WITH A PERFUSABLE VASCULATURE IN A MICROFLUIDIC DEVICE Reviewed

    Yuji Nashimoto, Itsuki Kunita, Akiko Nakamasu, Yu-suke Torisawa, Masamune Nakayama, Hidetoshi Kotera, Koichi Nishiyama, Takashi Miura, Ryuji Yokokawa

    30TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2017)   592 - 595   2017.4

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    In this study, we developed a microfluidic platform for a three-dimensional tissue model with a perfusable capillary network, which will allow, for the first time, a perfusion-culture in a tissue model with a high cell density. Our group previously reported that a spheroid of lung fibroblasts induced angiogenic sprouts from microchannels [1]. In this study, we successfully connected angiogenic sprouts to the vessel-like hollow structure in a spheroid and perfused the formed vascular network through microfluidic channels to the spheroid. This model opens up new techniques for tissue-culture for long-term.

    DOI: 10.1109/MEMSYS.2017.7863476

  • Reelin transiently promotes N-cadherin–dependent neuronal adhesion during mouse cortical development Reviewed International journal

    Yuki Matsunaga, Mariko Noda, Hideki Murakawa, Kanehiro Hayashi, Arata Nagasaka, Seika Inoue, Takaki Miyata, Takashi Miura, Ken-ichiro Kubo, Kazunori Nakajima

    Proceedings of the National Academy of Sciences   114 ( 8 )   2048 - 2053   2017.2

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    Reelin is an essential glycoprotein for the establishment of the highly organized six-layered structure of neurons of the mammalian neocortex. Although the role of Reelin in the control of neuronal migration has been extensively studied at the molecular level, the mechanisms underlying Reelin-dependent neuronal layer organization are not yet fully understood. In this study, we directly showed that Reelin promotes adhesion among dissociated neocortical neurons in culture. The Reelin-mediated neuronal aggregation occurs in an N-cadherin–dependent manner, both in vivo and in vitro. Unexpectedly, however, in a rotation culture of dissociated neocortical cells that gradually reaggregated over time, we found that it was the neural progenitor cells [radial glial cells (RGCs)], rather than the neurons, that tended to form clusters in the presence of Reelin. Mathematical modeling suggested that this clustering of RGCs could be recapitulated if the Reelin-dependent promotion of neuronal adhesion were to occur only transiently. Thus, we directly measured the adhesive force between neurons and N-cadherin by atomic force microscopy, and found that Reelin indeed enhanced the adhesiveness of neurons to N-cadherin; this enhanced adhesiveness began to be observed at 30 min after Reelin stimulation, but declined by 3 h. These results suggest that Reelin transiently (and not persistently) promotes N-cadherin–mediated neuronal aggregation. When N-cadherin and stabilized β-catenin were overexpressed in the migrating neurons, the transfected neurons were abnormally distributed in the superficial region of the neocortex, suggesting that appropriate regulation of N-cadherin–mediated adhesion is important for correct positioning of the neurons during neocortical development.

    DOI: 10.1073/pnas.1615215114

  • A new mathematical model for pattern formation by cranial sutures Reviewed

    Kenji Yoshimura, Ryo Kobayashi, Tomohisa Ohmura, Yoshinaga Kajimoto, Takashi Miura

    Journal of Theoretical Biology   408   66 - 74   2016.11

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    DOI: 10.1016/j.jtbi.2016.08.003

  • Theoretical Models of Vascular Pattern Formation. Reviewed

    Takashi Miura

    Fukuoka igaku zasshi = Hukuoka acta medica   107 ( 9 )   161 - 168   2016.9

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    Theoretical Models of Vascular Pattern Formation.

    DOI: 10.15017/1787037

  • Notch-mediated lateral inhibition regulates proneural wave propagation when combined with EGF-mediated reaction diffusion Reviewed

    Makoto Sato, Tetsuo Yasugi, Yoshiaki Minami, Takashi Miura, Masaharu Nagayama

    Proceedings of the National Academy of Sciences   113 ( 35 )   E5153 - E5162   2016.8

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    Notch-mediated lateral inhibition regulates binary cell fate choice, resulting in salt and pepper patterns during various developmental processes. However, how Notch signaling behaves in combination with other signaling systems remains elusive. The wave of differentiation in the <italic>Drosophila</italic> visual center or “proneural wave” accompanies Notch activity that is propagated without the formation of a salt and pepper pattern, implying that Notch does not form a feedback loop of lateral inhibition during this process. However, mathematical modeling and genetic analysis clearly showed that Notch-mediated lateral inhibition is implemented within the proneural wave. Because partial reduction in EGF signaling causes the formation of the salt and pepper pattern, it is most likely that EGF diffusion cancels salt and pepper pattern formation in silico and in vivo. Moreover, the combination of Notch-mediated lateral inhibition and EGF-mediated reaction diffusion enables a function of Notch signaling that regulates propagation of the wave of differentiation.

    DOI: 10.1073/pnas.1602739113

  • Tissue culture on a chip: Developmental biology applications of self-organized capillary networks in microfluidic devices Reviewed

    Takashi Miura, Ryuji Yokokawa

    Development, Growth & Differentiation   58 ( 6 )   505 - 515   2016.8

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    DOI: 10.1111/dgd.12292

  • Rat Articular Cartilages Change Their Tissue and Protein Compositions During Perinatal Period Reviewed International journal

    M. Kobayashi-Miura, T. Miura, H. Osago, Y. Yamaguchi, T. Aoyama, T. Tanabe, K.-i. Matsumoto, Y. Fujita

    Anatomia, Histologia, Embryologia   45 ( 1 )   9 - 18   2016.2

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    Articular cartilage (AC) covers the surface of bones in joints and functions as a cushion against mechanical loading. The tissue contains abundant extracellular matrix (ECM), which mainly consists of proteoglycans (PG) and collagen (COL) fibres. The property of AC is gradually changing by ageing with gravity loading. To know the property change of AC by initial gravity loading during short period after birth, we performed histological assays and proteomics assay on the AC of the femoral condyle in knee joints of perinatal rats. The water content (&#37;) was significantly decreased in neonate AC compared with fetal AC. During the perinatal stages (E19 and P0), the localizations of glycosaminoglycan (GAG) and type I and II COLs were homogeneous. The density of chondrocytes was significantly decreased in the deeper layers comparing with the surface layer in neonate AC. In addition, we found a drastic change in the protein expression pattern on proteomic analysis. The expressions of ECM components were relatively increased in neonate AC compared with fetal AC.

    DOI: 10.1111/ahe.12165

  • VASCULAR NETWORK FORMATION FOR A LONG-TERM SPHEROID CULTURE BY CO-CULTURING ENDOTHELIAL CELLS AND FIBROBLASTS Reviewed

    Tomoya Hayashi, Hisako Takigawa-Imamura, Koichi Nishiyama, Hirofumi Shintaku, Hidetoshi Kotera, Takashi Miura, Ryuji Yokokawa

    2015 28TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2015)   476 - 479   2015.4

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    In this paper, we present a poly-dimethylsiloxane (PDMS) microfluidic device to create a vascular network for a long-term spheroid culture, of which network and spheroid are consist of human umbilical vein endothelial cells (HUVEC) and normal human lung fibroblasts (LF), respectively. Following device design, fabrication, and fundamental evaluation of HUVEC sprouting conditions, we visualized that HUVEC networks were successfully formed by the co-culture with LFs and reached a LF-based spheroid. Moreover, perfusability of the network was evaluated by injecting fluorescent microbeads. This platform will be applicable for long-term tissue cultures to understand morphogenesis and modeling of blood vessel functions.

  • Models of lung branching morphogenesis Reviewed

    Takashi Miura

    Journal of Biochemistry   157 ( 3 )   121 - 127   2015.3

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    Vertebrate airway has a tree-like-branched structure. This structure is generated by repeated tip splitting, which is called branching morphogenesis. Although this phenomenon is extensively studied in developmental biology, the mechanism of the pattern formation is not well understood. Conversely, there are many tree-like structures in purely physical or chemical systems, and their pattern formation mechanisms are well-understood using mathematical models. Recent studies correlate these biological observations and mathematical models to understand lung branching morphogenesis. These models use slightly different mechanisms. In this article, we will review recent progress in modelling lung branching morphogenesis, and future directions to experimentally verify the models.

    DOI: 10.1093/jb/mvu087

  • Anatomical study for SLAP lesion repair Reviewed

    Ryuzo Arai, Masahiko Kobayashi, Hideto Harada, Hiroyuki Tsukiyama, Takahiko Saji, Yoshinobu Toda, Yoshihiro Hagiwara, Takashi Miura, Shuichi Matsuda

    Knee Surgery, Sports Traumatology, Arthroscopy   22 ( 2 )   435 - 441   2014.2

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    Purpose: The purpose of this study was to meticulously observe the structures around the origin of the long head of the biceps tendon (LHB) in order to propose a method of anatomical superior labrum anterior and posterior repair. Methods: Twenty-eight shoulders of 16 cadavers with intact LHB origin were macroscopically investigated. Among them, 20 shoulders with an intact superior labrum were additionally observed, to determine whether the anterior edge of LHB on the labrum (point 'A') was anterior to the supraglenoid tubercle. Serial sections vertical to LHB were observed using ordinary light and polarized microscopy in three glenoids and scanning acoustic microscopy in one. Results: The labrum had a meniscal appearance, and no LHB fibre was sent anterior to the anterior edge of the supraglenoid tubercle. 'A' was not located more posterior than the supraglenoid tubercle. All specimens had the so-called 'the sheet-like structure', in which the portion closer to the LHB origin tends to be stiffer. Fibres of the sheet-like structure ran vertically to LHB. Conclusion: Fibre orientation and the stiffness of the sheet-like structure suggest its support of LHB. As LHB fibres do not anteriorly cross over 'A', 'A' could be a landmark for the anterior border of LHB, independent from the sheet-like structure. Considering a previous report mentioning that the horizontal mattress suture maintains the meniscus-like structure which might be sufficient for proper motion of the normal superior labrum, the horizontal mattress suture not crossing over 'A' should be recommended from the viewpoint of functional anatomy. © 2013 Springer-Verlag Berlin Heidelberg.

    DOI: 10.1007/s00167-013-2385-3

  • [What we can see through mathematical modeling of biological pattern formation] Reviewed

    Takashi Miura

    Fukuoka igaku zasshi = Hukuoka acta medica   2013.4

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  • Turing and Wolpert Work Together During Limb Development Reviewed

    Takashi Miura

    Science Signaling   6 ( 270 )   pe14 - pe14   2013.4

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    DOI: 10.1126/scisignal.2004038

  • Fiber components of the shoulder superior labrum Reviewed

    Ryuzo Arai, Masahiko Kobayashi, Yoshinobu Toda, Shinichiro Nakamura, Takashi Miura, Takashi Nakamura

    Surgical and Radiologic Anatomy   34 ( 1 )   49 - 56   2012.1

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    DOI: 10.1007/s00276-011-0840-8

  • Application of reaction-diffusion equation to biological pattern formation Reviewed

    Miura, T., Nagayama, M.

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   2010.4

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  • In vitro vasculogenesis models revisited - Measurement of VEGF diffusion in matrigel Reviewed

    T. Miura, R. Tanaka

    Mathematical Modelling of Natural Phenomena   4 ( 4 )   118 - 130   2009.1

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    The circulatory system is one of the first to function during development. The earliest event in the system's development is vasculogenesis, whereby vascular progeniter cells form clusters called blood islands, which later fuse to form capillary networks. There exists a very good in vitro system that mimics this process. When HUVECs (Human Umbilical Vein Endothelial Cells) are cultured on Matrigel, they spontaneously form a capillary network structure. Two theoretical models have been proposed to explain the pattern formation of this in vitro system. Both models utilize chemotaxis to generate spatial instability, and one model specifies VEGF as the chemoattractant. However, there are several unknown factors concerning the experimental model. First, the pattern formation process occurs at the interface between the liquid medium and Matrigel, and it is unclear whether diffusion in the liquid or gel is critical. Second, the diffusion coefficient of VEGF, which determines the spatial scale of the capillary structure, has not been properly measured. In the present study, we modified the experimental system to clarify the effect of diffusion in Matrigel, and experimentally measured the diffusion coefficient of VEGF in this system. The relationship with the spatial scale of the pattern generated is discussed. © EDP Sciences, 2009.

    DOI: 10.1051/mmnp/20094404

  • Hedgehog signaling is involved in development of the neocortex Reviewed

    Masakazu Komada, Hiroto Saitsu, Masato Kinboshi, Takashi Miura, Kohei Shiota, Makoto Ishibashi

    Development   135 ( 16 )   2717 - 2727   2008.7

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    DOI: 10.1242/dev.015891

  • Modeling Lung Branching Morphogenesis Reviewed

    Takashi Miura

    Current Topics in Developmental Biology   291 - 310   2008.4

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    DOI: 10.1016/s0070-2153(07)81010-6

  • Mathematical analysis of a free-boundary model for lung branching morphogenesis Reviewed

    Dirk Hartmann, Takashi Miura

    Mathematical Medicine and Biology: A Journal of the IMA   24 ( 2 )   209 - 224   2007.6

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    DOI: 10.1093/imammb/dql029

  • [Turing reaction-diffusion model in developmental biology: application to limb skeletal pattern formation]. Reviewed

    Takashi Miura

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   50 ( 15 )   1955 - 1961   2005.12

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    [Turing reaction-diffusion model in developmental biology: application to limb skeletal pattern formation].

  • Disruption of actin cytoskeleton and anchorage-dependent cell spreading induces apoptotic death of mouse neural crest cells cultured in vitro Reviewed

    Atsushi Hinoue, Toshiya Takigawa, Takashi Miura, Yoshihiko Nishimura, Shigehiko Suzuki, Kohei Shiota

    Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology   282 ( 2 )   130 - 137   2005.2

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    In vertebrate embryos, neural crest cells emigrate out of the neural tube and contribute to the formation of a variety of neural and nonneural tissues. Some neural crest cells undergo apoptotic death during migration, but its biological significance and the underlying mechanism are not well understood. We carried out an in vitro study to examine how the morphology and survival of cranial neural crest (CNC) cells of the mouse embryo are affected when their actin cytoskeleton or anchorage-dependent cell spreading is perturbed. Disruption of actin fiber organization by cytochalasin D (1 μg/ml) and inhibition of cell attachment by matrix metalloproteinase-2 (MMP-2
    2.0 units/ml) were followed by morphologic changes and apoptotic death of cultured CNC cells. When the actin cytoskeleton was disrupted by cytochalasin D, the morphologic changes of cultured CNC cells preceded DNA fragmentation. These results indicate that the maintenance of cytoskeleton and anchorage-dependent cell spreading are required for survival of CNC cells. The spatially and temporally regulated expression of proteinases may be essential for the differentiation and migration of neural crest cells. © 2005 Wiley-Liss, Inc.

    DOI: 10.1002/ar.a.20150

  • A mesenchyme-free culture system to elucidate the mechanism of otic vesicle morphogenesis Reviewed

    Takashi Miura, Kohei Shiota, Gillian Morriss-Kay

    Journal of Anatomy   205 ( 4 )   297 - 312   2004.10

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    DOI: 10.1111/j.0021-8782.2004.00335.x

  • Periodic pattern formation in reaction-diffusion systems: An introduction for numerical simulation Reviewed

    Takashi Miura, Philip K. Maini

    Anatomical Science International   79 ( 3 )   112 - 123   2004.9

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    The aim of the present review is to provide a comprehensive explanation of Turing reaction-diffusion systems in sufficient detail to allow readers to perform numerical calculations themselves. The reaction-diffusion model is widely studied in the field of mathematical biology, serves as a powerful paradigm model for self-organization and is beginning to be applied to actual experimental systems in developmental biology. Despite the increase in current interest, the model is not well understood among experimental biologists, partly because appropriate introductory texts are lacking. In the present review, we provide a detailed description of the definition of the Turing reaction-diffusion model that is comprehensible without a special mathematical background, then illustrate a method for reproducing numerical calculations with Microsoft Excel. We then show some examples of the patterns generated by the model. Finally, we discuss future prospects for the interdisciplinary field of research involving mathematical approaches in developmental biology.

    DOI: 10.1111/j.1447-073x.2004.00079.x

  • Stage-dependency of the effect of ethanol on chondrogenic differentiation in mouse limb micromass culture

    Miura T., Kobayashi M., Shiota K.

    Congenital anomalies   41 ( 3 )   249 - 250   2001.9

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    Stage-dependency of the effect of ethanol on chondrogenic differentiation in mouse limb micromass culture

  • A novel method for analysis of the periodicity of chondrogenic patterns in limb bud cell culture: correlation of in vitro pattern formation with theoretical models Reviewed

    Takashi Miura, Masaru Komori, Kohei Shiota

    Anatomy and Embryology   201 ( 5 )   419 - 428   2000.4

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    DOI: 10.1007/s004290050329

  • Time-lapse observation of branching morphogenesis of the lung bud epithelium in mesenchyme-free culture and its relationship with the localization of actin filaments Reviewed

    Takashi Miura, Kohei Shiota

    International Journal of Developmental Biology   44 ( 8 )   899 - 902   2000.4

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    It has been shown that branching morphogenesis of the lung bud is mediated by epithelial-mesenchymal interaction via such molecules as FGF10, BMP4 and Shh. However, a recent study showed that the isolated lung epithelium still undergoes branching morphogenesis in vitro even in the absence of mesenchyme (Nogawa and Ito, 1995). In the present study, we observed in vitro the dynamic movement of the isolated lung epithelium of the fetal mouse using time-lapse recording, and investigated the roles of actin filaments in branching of the lung bud. First, time-lapse observation of the initial phase of lung branching morphogenesis revealed that at the sites of cleft formation, the epithelial surface was retracted inward from its original position. From this observation we assumed that there should be some structures which exert a physical force on the epithelium, and the localization and arrangement of actin fibers in the cultured lung epithelium were examined at various stages of branching morphogenesis. At the prebudding (6 h) and onset-budding (24 h) stages, no specific localization of actin filaments was observed in the lung bud epithelium, but at the postbudding stage (48 h) they were localized densely in the cells at the tip of the branched lung epithelium. The cell density was not different between the tip and cleft regions of the lung bud epithelium. When cultured with FGF-soaked beads, an actin-rich region was induced at the tip of the lung bud which was growing toward an FGF-soaked bead. These results indicate that actin fibers do not play a significant part in cleft formation but can be secondarily induced by FGF in the surrounding matrix and play some roles at later shaping of the branch in lung morphogenesis.

  • Extracellular matrix environment influences chondrogenic pattern formation in limb bud micromass culture: Experimental verification of theoretical models Reviewed

    Takashi Miura, Kohei Shiota

    The Anatomical Record   258 ( 1 )   100 - 107   2000.1

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    DOI: 10.1002/(sici)1097-0185(20000101)258:1<100::aid-ar11>3.0.co;2-3

  • Computerized three-dimensional reconstruction of human embryos and their organs using the "NIH image" software Reviewed

    Takashi Miura, Masaru Komori, Tadashi Takahashi, Kohei Shiota

    Kaibogaku zasshi. Journal of anatomy   70 ( 4 )   353 - 361   1995.4

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Books

  • 発生の数理

    三浦, 岳

    京都大学学術出版会  2015.12 

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    Responsible for pages:総ページ数:v, 220p   Language:Japanese  

  • 発生の数理

    三浦 岳(Role:Sole author)

    2015.12 

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  • チューリング : 総特集 = A. M. Turing

    三浦 岳

    青土社  2012.10 

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    Responsible for pages:総ページ数:222p   Language:Japanese  

Presentations

  • 培養細胞のパターン形成

    三浦 岳

    岡山駅前セミナー  2014.12 

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    Venue:岡山   Country:Other  

  • 細胞運動と Delta-Notch の相互作用の解析

    小熊 俊輝, 三浦 岳

    定量生物学の会  2015.1 

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    Venue:福岡   Country:Other  

  • 生物のかたちづくりのモデリングの歴史:単純と複雑の循環 Invited

    三浦 岳

    定量生物学の会  2015.1 

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    Venue:福岡   Country:Other  

  • 血管網形成の数理モデル Invited

    三浦 岳

    次世代創薬シンポジウム  2015.3 

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    Venue:京都   Country:Other  

  • Modeling Pattern formation during development_

    Takashi Miura

    熊本大学講義  2015.6 

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    Venue:熊本   Country:Other  

  • Mechanism of shortcut prevention during retinal blood vessel development Invited

    Atsushi Tero, Akiyoshi Uemura, Takashi Miura

    発生生物学会  2015.6 

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    Venue:茨城   Country:Other  

    Perfusable synthetic self-organized vascular network - experiment and modeling

  • 縫合線パターンのフラクタル性の起源

    三浦 岳

    Craniosynostosis研究会  2015.7 

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    Venue:大阪   Country:Other  

  • Modeling endothelial capillary network formation in microdevice

    Takashi Miura

    数理生物学会  2015.8 

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    Venue:京都   Country:Other  

  • Mathematical Modeling of MDCK spontaneous movement

    Daiki Sasaki, Takashi Miura

    数理生物学会  2015.8 

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    Venue:京都   Country:Other  

  • Formation of fractal structure in skull suture

    Takashi Miura

    ICIAM  2015.8 

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    Venue:Beijing   Country:Other  

  • 体外で機能する毛細血管網の形成 - モデリングによる理解と器官培養系への応用

    三浦 岳

    大阪母子センターセミナー  2015.9 

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    Venue:大阪   Country:Other  

  • Modeling vasculogenesis in perfusable 3D microvascular networks on a chip

    Takashi Miura

    Asia Pacific Developmental Biology Conference  2015.9 

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    Venue:Xi'an   Country:Other  

  • MDCK細胞の集団運動における細胞間相互作用

    三浦 岳

    応用数理学会  2015.9 

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    Venue:金沢   Country:Other  

  • 発生におけるマルチスケールの自発的パターン形成現象

    三浦 岳

    富山シンポジウム  2015.11 

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    Venue:富山   Country:Other  

  • 発生におけるマルチスケールのパターン形成現象

    三浦 岳

    数理研研究集会「現象解明に向けた数値解析学の新展開」  2015.11 

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    Venue:京都   Country:Other  

  • 生物のかたちを数学で理解する Invited

    三浦 岳

    白石記念講座  2015.11 

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    Venue:東京   Country:Other  

  • Mechanism of epithelial pattern formation in development: experiment and modeling Invited

    Takashi Miura

    International Conference on Systems Biolofy  2015.11 

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    Venue:Singapore   Country:Other  

  • Perfusable self-organized capillary network in a microdevice: modeling and applications

    Takashi Miura

    熊本大学  2015.12 

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    Venue:熊本   Country:Other  

  • 頭蓋骨縫合線のパターン形成とフラクタル性

    三浦 岳

    京都大学理学部  2016.2 

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    Venue:京都   Country:Other  

  • 体外で機能する毛細血管網の形成 - モデリングによる理解と器官培養系への応用

    三浦 岳

    解剖学会  2016.3 

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    Venue:福島   Country:Other  

  • Mechanism of Scaling law formation in developing retina vasculature structure

    Osamu Iizuka, Akiyoshi Uemura, Hiroshi Kori, Takashi Miura

    発生生物学会特別シンポジウム  2016.6 

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    Venue:東京   Country:Other  

  • Mathematical analysis of fractality of skull sutures

    Yuto Naroda, Yoshinaga Kajimoto, Kenji Yoshimura, Takashi Miura

    発生生物学会特別シンポジウム  2016.6 

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    Venue:東京   Country:Other  

    Mechanism of scaling law formation in retina vasculature - modeling and experiment

  • 血管網形成の数理モデルと実験的検証 Invited

    三浦 岳

    生物リズム若手の会  2016.9 

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    Venue:山口   Country:Other  

    Pericyte coverage of endothelial cells: in vitro experiments and computational modeling.

  • The effect of cell dynamics on Delta-Notch pattern formation during retinal angiogenesis

    Toshiki Oguma, Tomoharu Shinoda, Shuntaro Ogura, Akiyoshi Uemura, Takaki Miyata, Takashi Miura

    数理生物学会  2016.9 

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    Venue:福岡   Country:Other  

    Modeling of skull suture pattern formation

  • Modeling pattern formation of skull suture interdigitation

    Takashi Miura

    Mathematics of Pattern Formation  2016.9 

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    Venue:Poland   Country:Other  

    Mechanism of skull suture pattern formation

  • Mechanism of jigsaw-puzzle pattern formation in plant leaf epidermal cell: modeling single cell shape by interface equation and convolution kernel

    Takashi Miura, Takumi Higaki, Natsumaro Kutsuna, Kae Akita, Hisako Takigawa-Imamura, Kenji Yoshimura

    数理生物学会  2016.9 

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    Venue:福岡   Country:Other  

  • Mechanism of Scaling law formation in developing retina vasculature

    Osamu Iizuka, Akiyoshi Uemura, Takashi Miura

    数理生物学会  2016.9 

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    Venue:福岡   Country:Other  

  • Mathematical analysis of fractality of skull sutures

    Yuto Naroda, Kenji Yoshimura, Yoshinaga Kajimoto, Tomohisa Ohmura, Takashi Miura

    数理生物学会  2016.9 

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    Venue:福岡   Country:Other  

    Distribution pattern of immune cells - quantification and mathematical modeling of pattern formation mechanism

  • Mathematical analysis of fractality of skull sutures

    Yuto Naroda, Kenji Yoshimura, Yoshinaga Kajimoto, Tomohisa Ohmura, Takashi Miura

    数理生物学会  2016.9 

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    Venue:福岡   Country:Other  

  • 頭蓋骨縫合線のパターン形成

    三浦 岳

    六甲医学研究会  2016.10 

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    Venue:淡路島   Country:Other  

  • 灌流可能な自己組織化血管網

    三浦 岳

    金沢大学先魁プロジェクト  2016.11 

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    Venue:金沢   Country:Other  

  • Vascular pattern formation: experiment, theory and reconstruction

    Takashi Miura

    京都大学講義  2016.11 

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    Venue:京都   Country:Other  

  • 反応拡散系を用いた自発的パターン形成の数理モデル化

    三浦 岳

    京都大学医学部皮膚科  2016.12 

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    Venue:京都   Country:Other  

  • Modeling vascular pattern formation in retina vasculature

    Takashi Miura

    血管生物医学会  2016.12 

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    Venue:長崎   Country:Other  

  • Mechanism of jigsaw-puzzle pattern formation in plant leaf epidermal cell

    Takashi Miura

    分子生物学会  2016.12 

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    Venue:横浜   Country:Other  

  • 細胞壁と頭蓋骨 - 数理モデルによる 自発的パターン形成メカニズムの解明

    三浦岳

    基礎生物学研究所セミナー  2017.1 

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    Venue:岡崎   Country:Other  

  • 血管内皮細胞による自律的なパターン形成の数理

    ◯中益朗子,出原浩史,新宮直人,三浦岳

    第4回JST数学領域横断若手合宿  2017.2 

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    Venue:香川   Country:Other  

    Establishment of perfusable vascular network for understanding multicellular pattern formation

  • 血管内皮細胞が自律的に形成するパターンサイズに対する数理解析

    Akiko Nakamasu, Masamune Nakayama, Naoto Shingu, Hirofumi Izuhara, Yuji Nashimoto, Itsuki Kunita, Yuichiro Arima, Koichi Nishiyama, Ryuji Yokokawa, Takashi Miura

    発生生物学会  2017.5 

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    Venue:東京   Country:Other  

    Analyses of sizes of self-organized patterns composed by endothelial cell

  • 網膜血管網におけるスケーリング則の形成メカニズム

    Shotaro Kawamura, Atsushi Tero, Hiroshi Kori, Akiyoshi Uemura, Takashi Miura

    発生生物学会  2017.5 

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    Venue:東京   Country:Other  

    Modeling vascular scaling law formation in retina vasculature

  • 生物の形づくりの数理 -Mathematicaによるrapid prototyping- Invited

    三浦 岳

    Wolfram Conference  2017.6 

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    Venue:福岡   Country:Other  

  • 精細管における wavetrain パターン形成のモデリング

    河村真理, 杉原圭, 三浦岳

    数理生物学会  2017.10 

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    Venue:札幌   Country:Other  

  • 人工血管網形成の数理モデル

    佐々木大貴, 三浦岳

    数理生物学会  2017.10 

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    Venue:札幌   Country:Other  

  • Skull suture and plant cell wall: Mechanism of interdigitation pattern formation

    三浦岳

    Patterns and dynamics with nonlocal effects (定山渓)  2017.10 

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    Venue:札幌   Country:Other  

    Skull suture and plant cell wall: Mechanism of interdigitation pattern formation

  • Mechanism of scaling law formation in retina vasculature - modeling and experiment

    Osamu Iizuka, Shotaro Kawamura, Akiyoshi Uemura, Takashi Miura

    数理生物学会  2017.10 

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    Venue:札幌   Country:Other  

    Mechanism of scaling law formation in retina vasculature - modeling and experiment

  • FGFとWntの協同による肺分岐のヒエラルキー構造形成

    今村(滝川)寿子, 麓勝己, 三浦岳

    数理生物学会  2017.10 

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    Venue:札幌   Country:Other  

  • A Mathematical model to generate fractal structure in skull suture

    Yuto Naroda, Yoshie Endo, Kenji Yoshimura, Takashi Miura

    数理生物学会  2017.10 

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    Venue:札幌   Country:Other  

    A Mathematical model to generate fractal structure in skull suture

  • Mechanism of skull suture pattern formation

    三浦岳

    IWOMB2018 (Cebu)  2018.1 

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    Venue:Cebu   Country:Other  

    Mechanism of skull suture pattern formation

  • 植物細胞壁の湾曲構造形成の 理論モデル

    三浦岳 桧垣匠

    AIMaP「反応拡散系と実験の融合」  2018.2 

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    Venue:金沢   Country:Other  

  • Pericyte coverage of endothelial cells: in vitro experiments and computational modeling.

    Kei Sugihara, Saori Sasaki, Akiyoshi Uemura, Satoru Kidoaki, Takashi Miura

    日本発生生物学会  2018.6 

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    Venue:東京   Country:Other  

    Pericyte coverage of endothelial cells: in vitro experiments and computational modeling.

  • 血の通った培養系: マイクロ流体デバイスと 自己組織化血管網の融合

    三浦 岳

    細胞凝集研究会  2018.7 

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    Venue:酒田   Country:Other  

  • 細胞壁と頭蓋骨: 分野を超える数理 Invited

    三浦岳

    生物数学の理論とその応用  2018.9 

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    Venue:京都   Country:Other  

  • Modeling of skull suture pattern formation

    三浦岳

    生物物理学会  2018.9 

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    Venue:岡山   Country:Other  

    Modeling of skull suture pattern formation

  • Distribution pattern of immune cells - quantification and mathematical modeling of pattern formation mechanism

    三浦岳

    日本生化学会  2018.9 

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    Venue:京都   Country:Other  

    Distribution pattern of immune cells - quantification and mathematical modeling of pattern formation mechanism

  • Establishment of perfusable vascular network for understanding multicellular pattern formation

    三浦岳

    日本分子生物学会  2018.11 

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    Venue:横浜   Country:Other  

    Establishment of perfusable vascular network for understanding multicellular pattern formation

  • 肺の枝分れの形成機構 - 数理モデリングによる理解 Invited

    三浦 岳

    呼吸器イメージング学会  2019.1 

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    Venue:東京   Country:Other  

  • 頭蓋骨縫合線のパターン形成

    三浦 岳

    反応拡散系 のパターン形成とその応用  2019.2 

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    Venue:岡山   Country:Other  

  • 口蓋縫合のパターン形成の数理モデル

    澁澤伸英, 三浦岳

    日本解剖学会  2019.3 

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    Venue:新潟   Country:Other  

  • 頭蓋骨縫合線の形成機構

    三浦 岳

    第2回松江数理生物学・現象数理学ワークショップ  2019.8 

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    Venue:島根   Country:Other  

  • 血管網のパターン形成:数理モデリングと再構成による検証 Invited

    三浦 岳

    数理シグナル若手の会  2019.9 

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    Venue:愛媛   Country:Other  

  • 細胞集団運動における周期的突起形成のメカニズム

    小熊俊輝, 今村寿子, 三浦岳

    日本数理生物学会年会  2019.9 

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    Country:Other  

  • 小腸粘膜の三次元構造は上皮細胞をムラなく脱落させる

    甲斐悠斗, 三浦岳

    数理生物学会  2019.9 

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    Venue:東京   Country:Other  

  • Perfusable synthetic self-organized vascular network - experiment and modeling Invited

    三浦 岳

    International Conference of Systems Biology  2019.11 

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    Venue:沖縄   Country:Other  

    Perfusable synthetic self-organized vascular network - experiment and modeling

  • 上皮細胞シートにおけるフラクタル構造形成のメカニズム

    小熊 俊輝, 今村 寿子, 三浦 岳

    第16回 生物数学の理論とその応用 -生命現象の定量的理解に向けて  2020.1 

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  • ペリサイトが血管分岐部を選好するメカニズムの探索

    杉原圭, 植村明嘉, 三浦岳

    日本数理生物学会  2020.9 

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    Country:Other  

  • 頭蓋骨縫合線のパターン形成機構 Invited

    三浦 岳

    東京医科歯科大学 数理生物学セミナー2020  2020.11 

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    Country:Other  

  • 血管網のパターン形成:実験、数理モデリングと再構成

    三浦岳

    北大MMCセミナー  2021.1 

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    Country:Other  

  • Self-organized perfusable capillary network in vitro: Experiment and modeling

    Takashi Miura

    Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture  2021.3 

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    Country:Other  

  • 生物の形作りとシミュレーション:分野間連携の実際 Invited

    三浦 岳

    NINSコロキウム  2014.12 

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    Venue:小田原   Country:Other  

  • マウス四肢原基微小集積培養系における軟骨形成パターンのメカニズム Invited

    三浦 岳

    日本解剖学会  2000.3 

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  • 四肢間葉細胞微小集積培養系の形態形成のメカニズム

    三浦 岳,塩田 浩平

    第33回日本発生生物学会  2000.5 

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  • Mechanisms of Pattern Formation in Limb Bud Micromass Culture: Possible Relationship with in vivo Pattern Formation

    Takashi Miura

    先天異常学会  2000.7 

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    Mechanisms of Pattern Formation in Limb Bud Micromass Culture: Possible Relationship with in vivo Pattern Formation

  • Morphogenesis of Embryonic Lung Epithelium in Vitro: Possible Relationship with Theoretical Models

    Takashi Miura

    日伊応用数理joint meeting Biology and Medicine Moving Towards Mathematics  2000.11 

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    Morphogenesis of Embryonic Lung Epithelium in Vitro: Possible Relationship with Theoretical Models

  • マウス四肢原基微小集積培養系における軟骨パターン形成のメカニズム Invited

    三浦 岳

    日本先天異常学会  2001.7 

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  • Role of actin filaments in branching morphogenesis of the embryonic lung epithelium in vitro

    Takashi Miura

    14th ICDB  2001.7 

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    Role of actin filaments in branching morphogenesis of the embryonic lung epithelium in vitro

  • Speed of pattern appearance in reaction-diffusion system

    Takashi Miura

    Mathematical Understanding of Complex Patterns in the Life Sciences  2003.3 

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    Speed of pattern appearance in reaction-diffusion system

  • Mesenchyme-free otic vesicle culture to elucidate the mechanism of morphogenesis

    Takashi Miura, Kohei Shiota, Gillian Morriss-Kay

    Inner Ear Workshop  2003.9 

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  • Thin digits in the Doublefoot mutant limb: mixed-mode pattern in reaction-diffusion model of the growing domain

    Takashi Miura, GIllian Morriss-Kay, Philip, K. Maini

    SMB2004  2004.7 

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  • Manipulating expression of the ventral otic vesicle marker Pax-2 in a mesenchyme-free culture system

    Takashi Miura, Kohei Shiota, GIllian Morriss-Kay

    IFAA  2004.8 

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  • Digit splitting pattern in Doublefoot mutant mice limb buds - Relationship with reaction-diffusion model on growing domain Invited

    Takashi Miura

    日本数理生物学会  2004.9 

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  • Doublefoot mutant マウスにおける四肢の変異パターン – 拡大領域上での Turing パターンとの関連

    三浦 岳

    形の科学会  2005.6 

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  • Modelling dynamics of skull suture pattern formation during development

    Takashi Miura, Kohei Shiota

    日本先天異常学会  2005.7 

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    Modelling dynamics of skull suture pattern formation during development

  • 頭蓋骨縫合線の パターン形成のモデル化

    三浦 岳

    発生生物学会  2006.6 

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  • 頭蓋骨縫合線のパターン形成の数理モデル化とその実験的検証

    三浦岳

    かたちまつり2006  2006.11 

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  • Cyst-branch difference in chick lung

    Takashi Miura

    日本解剖学会  2007.3 

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    Cyst-branch difference in chick lung

  • Analysis of chick lung branching morphogenesis using mathematical model

    Takashi Miura, Kohei Shiota

    日本解剖学会  2007.3 

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  • Cyst-branch difference of chick lung results from different morphogen diffusion coefficient

    Takashi Miura

    発声生物学会  2007.5 

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  • Noninvasive observation of mouse skull using microCT

    Miura, T, Morimoto, N, Ogihara, N, Shiota K

    日本先天異常学会  2007.7 

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  • Mechanism of lung branching morphogenesis

    Takashi Miura

    Societr of Mathematical Biology Annual Meeting  2007.7 

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    Mechanism of lung branching morphogenesis

  • 発生に於ける形態形成現象をどうモデル化するか?

    三浦 岳

    宇治化研セミナー  2008.1 

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  • 生物の二つの「形」

    三浦 隆

    非平衡現象と協調現象の数理  2008.1 

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  • Time course observation of mouse skull development using microCT

    Takashi Miura

    解剖学会  2008.3 

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    Time course observation of mouse skull development using microCT

  • Modelling lung branching morphogenesis

    Takashi Miura

    現象数理セミナー  2008.4 

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    Modelling lung branching morphogenesis

  • 生物の形づくりのしくみ

    三浦 岳

    マイクロデバイス集中講義  2008.5 

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  • In vitro vasculogenesis model revisited: Measurement of VEGF diffusion

    Takashi Miura

    発生生物学会  2008.5 

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    In vitro vasculogenesis model revisited: Measurement of VEGF diffusion

  • 頭蓋骨の縫合線のパターン形成

    三浦 岳

    パターンダイナミクスの数理とその周辺  2008.6 

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  • Visualizing morphogen distribution in lumen

    Takashi Miura

    SDB meeting  2008.7 

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    Visualizing morphogen distribution in lumen

  • Mechanism of lung branching morphogenesis

    Takashi Miura

    金沢非線形現象研究会  2008.8 

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  • 肺の枝分かれ形成の メカニズム

    三浦 岳

    「複雑系情報学に基づく創発的なデータ生成過程のモデル化」に関する研究会  2008.9 

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  • 肺の枝分かれ構造の形成機構

    三浦 岳

    定量生物学の会  2009.1 

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  • 似てるものは同じ?

    三浦 岳

    数理研研究集会  2009.1 

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  • Mechanism of lung branching morphogenesis

    Takashi Miura

    生物数学の理論とその応用  2009.1 

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    Mechanism of lung branching morphogenesis

  • Expression of proximodistal markers during chick lung development

    Takashi Miura

    日本解剖学会  2009.3 

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    Expression of proximodistal markers during chick lung development

  • Expression of proximodistal markers during chick lung development

    Takashi Miura

    発生生物学会  2009.5 

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  • Expression of proximodistal markers during chick lung development

    Takashi Miura

    ISDB meeting  2009.9 

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    Expression of proximodistal markers during chick lung development

  • 自発的パターン形成における拡散と領域成長の役割:培養系での形態制御の可能性

    三浦 岳

    バイオマテリアル学会  2009.11 

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  • Mechamism of lung branching morphogenesis

    Takashi Miura

    日本生理学会  2010.5 

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    Mechamism of lung branching morphogenesis

  • Roles of epithelium and mesenchyme during lung branching morphogenesis

    Takashi Miura

    日本発生生物学会  2010.6 

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    Roles of epithelium and mesenchyme during lung branching morphogenesis

  • FGF-induced collective cell migration during lung branching morphogenesis

    Takashi Miura

    SDB-JSDB Joint Meeting in New Mexico  2010.8 

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  • Mechanism of lung branching morphogenesis

    Takashi Miura

    24th ALTENBERG WORKSHOP IN THEORETICAL BIOLOGY  2010.9 

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    Mechanism of lung branching morphogenesis

  • 発生における 形態形成のモデル化とその実験的検証

    三浦 岳

    明治大学集中講義  2010.11 

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  • in vitroでのVEGFの拡散係数計測

    吉村賢二, 三浦岳

    定量生物学の会  2010.11 

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  • 発生現象の数理モデル化とその実験的検証

    三浦 岳

    Potts model研究会  2010.12 

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  • Modeling lung branching morphogenesis in vitro

    Takashi Miura

    Far-From-Equilibrium-Dynamics 研究集会  2011.1 

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  • Modeling lung branching morphogenesis via epithelial-mesenchymal interaction

    Takashi Miura

    発生生物学会  2011.5 

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    Modeling lung branching morphogenesis via epithelial-mesenchymal interaction

  • 肺の枝分かれ構造の 形成機構

    三浦 岳

    京都駅前セミナー  2011.7 

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  • Modeling lung branching morphogenesis via epithelial-mesenchymal interaction

    Takashi Miura

    SDB annual meeting 2011  2011.7 

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    Modeling lung branching morphogenesis via epithelial-mesenchymal interaction

  • 上皮間葉間相互作用による 肺の枝分かれ構造形成

    三浦 岳

    数理生物学会  2011.9 

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  • Morphogen gradient

    三浦 岳

    RIMS研究集会  2011.9 

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  • How well does Turing's theory of morphogenesis work?

    三浦岳

    RIMS 研究集会 第8回生物数学の理論とその応用  2011.11 

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    How well does Turing's theory of morphogenesis work?

  • Turing パターンにおけるパターンの精度と生成速度の関係

    平賀顕一, 三浦岳

    定量生物学の会  2012.1 

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  • 頭蓋骨縫合線のパターン形成の数理モデル

    吉村賢二, 三浦岳

    日本解剖学会  2012.3 

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  • フェーズフィールド法を用いた肺の枝分かれ構造形成のモデル化

    三浦 岳

    解剖学会  2012.3 

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  • 頭蓋骨縫合線のパターン形成:発生と数理をつなぐ

    三浦 岳

    Evo-Devo若手の会  2012.6 

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  • Visualizing morphogen diffusion dynamics during lung branching morphogenesis in vitro.

    Takashi Miura

    FASEB lung meeting  2012.7 

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    Visualizing morphogen diffusion dynamics during lung branching morphogenesis in vitro.

  • 頭蓋骨縫合線のパターン形成の 数理モデル化とその実験的検証 Invited

    三浦 岳

    植物学会  2012.9 

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  • 内皮細胞のメッシュワーク形成と VEGFの拡散ダイナミクス

    三浦 岳

    数理生物学会  2012.9 

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  • Visualizing Morphogen Diffusion Dynamics in lung branching morphogenesis

    Takashi Miura

    APDB conference  2012.10 

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    Visualizing Morphogen Diffusion Dynamics in lung branching morphogenesis

  • 生物の形づくりのモデリング:プロトタイピング言語としての Mathematica Invited

    三浦 岳

    Wolfram Conference 2012  2012.11 

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  • A novel mathematical model of endothelial cell dynamics during angiogenic morphogenesis: analysis of cell-based mechanisms

    杉原 圭, 西山 功一, 三浦岳, 有馬 聡, 箱崎 勇治, 栗原 由紀子, 栗原 裕基

    分子生物学会  2012.12 

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    A novel mathematical model of endothelial cell dynamics during angiogenic morphogenesis: analysis of cell-based mechanisms

  • A New Mathematical Model of Pattern Formation of the Cranial Suture

    吉村賢二, 三浦岳

    分子生物学会  2012.12 

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    A New Mathematical Model of Pattern Formation of the Cranial Suture

  • 頭蓋骨縫合線のパターン形成の 数理モデル化とその実験的検証

    三浦 岳, 吉村 賢二

    植物ミニシンポジウム  2013.1 

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    Venue:熊本   Country:Other  

    Modeling pattern formation of skull suture interdigitation

  • 細胞壁と頭蓋骨 - パターン形成ダイナミクスの数理モデルによる統合的理解 Invited

    三浦 岳, 吉村 賢二

    植物細胞壁シンポジウム  2013.3 

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    Venue:仙台   Country:Other  

  • Cell Wall and Skull Bone:Understanding Pattern Formation Dynamics using Mathematical Model

    Takumi Higaki, Takashi Miura

    発生生物学会  2013.5 

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    Venue:松江   Country:Other  

    Mathematical model of epithelial buckling for single- and multi-step processes of the intestinal villus formation

  • 頭蓋骨縫合線のパターン形成の数理モデル Invited

    三浦 岳

    Craniosynostosis研究会  2013.7 

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    Venue:東京   Country:Other  

  • 肺の枝分れの形成機構とmorphogen の拡散動態

    三浦 岳

    CDBセミナー  2013.7 

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    Venue:神戸   Country:Other  

  • Modeling lung branching morphogenesis

    Takashi Miura

    第61回基礎生物学研究所研究集会  2013.7 

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    Venue:岡崎   Country:Other  

    Modeling vascular scaling law formation in retina vasculature

  • 発生における 自発的パターン形成

    三浦 岳

    細胞システムコロキウム  2013.9 

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    Venue:埼玉   Country:Other  

  • ショウジョウバエ神経発生におけるProneural waveの伝播現象

    三浦 岳, 長山 雅晴, 佐藤 純

    数理生物学会  2013.9 

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    Venue:静岡   Country:Other  

  • Mechanism of lung branching morphogenesis

    Takashi Miura

    3領域合同シンポジウム  2013.9 

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    Venue:名古屋   Country:Other  

  • Mechanism of lung branching morphogenesis

    Takashi Miura

    熊大リエゾンラボ研究会  2013.10 

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    Venue:熊本   Country:Other  

  • Mechanism of lung branching morphogenesis

    Takashi Miura

    生物物理学会  2013.10 

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    Venue:名古屋   Country:Other  

  • 細胞集団運動における振動現象

    三浦 岳

    時間生物学会  2013.11 

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    Venue:大阪   Country:Other  

    Modeling vascular scaling law formation in retina vasculature

  • 内皮細胞の自発的パターン形成 - ブレイクスルーを生むコラボレーション

    三浦 岳, 西山 功一

    発生生物学会秋季シンポジウム  2013.11 

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    Venue:大阪   Country:Other  

    Mechanism of jigsaw-puzzle pattern formation in plant leaf epidermal cell

  • 発生における自発的パターン形成とmorphogen の拡散動態

    三浦 岳

    顕微鏡学会九州支部会  2013.12 

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    Venue:福岡   Country:Other  

    Analyses of sizes of self-organized patterns composed by endothelial cell

  • Regulation of morphogen diffusion affects spontaneous pattern formation in development

    Takashi Miura

    発生生物学会  2014.5 

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    Venue:名古屋   Country:Other  

  • 肺の枝分れの形成機構 Invited

    三浦 岳

    分子病理学研究会宮城蔵王シンポジウム  2014.7 

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    Venue:仙台   Country:Other  

  • Morphogen diffusion and spontaneous pattern formation: from theory to practice

    Takashi Miura

    基礎生物学研究所サマースクール  2014.8 

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    Venue:岡崎   Country:Other  

  • 肺の枝分れ構造の形成機構 Invited

    三浦 岳

    佐島シンポジウム  2014.10 

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    Venue:京都   Country:Other  

  • Collective migration of lung epithelial cells during branching morphogenesis

    Takashi Miura

    生化学会  2014.10 

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    Venue:京都   Country:Other  

  • 食道蠕動運動の数理モデリング Invited

    三浦 岳

    平滑筋学会若手の会  2023.2 

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  • 頭蓋骨と植物細胞壁: 生物の形を数理モデルで理解する Invited

    三浦 岳

    日本生化学会  2023.10 

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  • 頭蓋骨と植物細胞壁 生物の形を数理モデルで理解する

    三浦 岳

    日本生化学会大会プログラム・講演要旨集  2023.10  (公社)日本生化学会

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  • 細胞のかたちづくりの原理に数理と実験で迫る 細胞間接着の彎曲構造の形成機構

    大谷 哲久, 三浦 岳

    日本生化学会大会プログラム・講演要旨集  2023.10  (公社)日本生化学会

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  • 細胞のかたちづくりの原理に数理と実験で迫る 細胞が細胞を包むしくみ 血管内皮細胞と周皮細胞の場合

    杉原 圭, 佐々木 沙織, 木戸秋 悟, 植村 明嘉, 三浦 岳

    日本生化学会大会プログラム・講演要旨集  2023.10  (公社)日本生化学会

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  • 中心窩無血管領域周辺でのヒト網膜血管パターンの数理モデル化

    吉村 公太朗, 杉原 圭, 丸子 一朗, 飯田 知弘, 三浦 岳

    日本生化学会大会プログラム・講演要旨集  2023.10  (公社)日本生化学会

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MISC

  • Macular imaging 病態のより深い理解へ

    飯田 知弘, 丸子 一朗, 長谷川 泰司, 丸子 留佳, 高木 まなみ, 内村 英子, 荒川 久弥, 河野 泰三, 高橋 洋平, 和泉 雄彦, 河合 萌子, 梯 瑞葉, 橋谷 臨, 鄭 雅心, 横山 達郎, 田口 晃一, 蔵並 藍, 山口 沙耶, 西原 聡一朗, 石龍 鉄樹, 古田 実, 齋藤 昌晃, 小島 彰, 菅野 幸紀, 小山田 紘, 小笠原 雅, 古泉 英貴, 岡田 アナベルあやめ, 片岡 恵子, 山本 亜希子, 中山 真紀子, 渡邊 裕斗, 向井 亮, 板垣 可奈子, 笠井 暁仁, 新竹 広晃, 本庄 純一郎, 寺尾 信宏, 玉城 環, 湧川 空子, 宮良 安宣, 森 隆三郎, 田中 公二, 若月 優, 小野江 元, 三浦 岳, 今村 寿子, 杉原 圭, 吉村 公太郎, Spaide Richard F., 今村 裕, 秋葉 正博, 山口 達夫, 廣瀬 僚一, 境原 学

    日本眼科学会雑誌   128 ( 3 )   159 - 196   2024.3   ISSN:0029-0203

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    Language:Japanese   Publisher:(公財)日本眼科学会  

    高度な視機能を司るためにきわめて精巧に「創られた」黄斑ではあるが,そこにはさまざまな病変が生じてしまう.なぜ,黄斑には病変が起こりやすいのか.Macular imagingを用いて,疑問を解決するために取り組んできた研究を紹介して,黄斑疾患の病態理解と治療への展開,そして今後の研究の展望を論じたい.I.光干渉断層計(OCT)による脈絡膜・強膜構造の解析OCTを用いて,長らくブラックボックスであった脈絡膜の構造解析を行った.中心性漿液性脈絡網膜症(CSC)に対する光線力学的療法(PDT)は,病的状態にある脈絡膜厚,脈絡膜血管径と血管透過性亢進を正常化させて,網膜下液を消失させることが分かった.これは脈絡膜形態が治療により変化して,治療効果と密接に関係することを示した最初の研究であった.ポリープ状脈絡膜血管症(PCV)でも脈絡膜の状態は疾患活動性に関与していた.新生血管型加齢黄斑変性(AMD)でもPDTや抗血管内皮増殖因子(VEGF)薬により脈絡膜厚は減少し,その程度は治療反応に連動しており,「脈絡膜所見に基づく新生血管型AMD治療戦略」という新しい考え方を提唱した.現在では臨床治験でも脈絡膜厚測定が行われるなど,その重要性が認識されてきている.Vogt-小柳-原田病(VKH病)では急性期に脈絡膜厚は著しく増加し,治療により急速に減少する.時に視神経炎などとの鑑別が困難な乳頭浮腫型VKH病でも同様であり,OCTによる脈絡膜観察は診断と治療効果判定に有用である.強度近視眼と傾斜乳頭症候群で,脈絡膜よりさらに深部にある強膜画像を解析して,黄斑部に限局した強膜肥厚があること,その形態異常がさまざまな黄斑病変を引き起こすことを示した.強度近視眼における眼軸長延長に伴う強膜伸展は均一ではなく,黄斑部には限局した強膜肥厚があり,視機能維持のために黄斑部での眼球形態を保持する機構が存在する可能性がある.II.超広角OCTの開発と病態解明へのアプローチ共同研究開発を進めているprototype超広角OCTでは,眼底後極部から赤道部までの幅約31.5mm,深さ約10.9mmの範囲を撮影可能である.裂孔原性網膜剥離など周辺部の網膜病変だけでなく,広範囲の脈絡膜構造・体積や眼球形態を捉えることができる.超広角OCTを用いてCSCを観察したところ,脈絡膜肥厚は後極部のみに限局しており,周辺部では正常眼と差がないことが分かった.これは,CSCでは後極部の脈絡膜に水分貯留が起こりやすい眼球形態をしていることを示唆し,なぜ黄斑部に発症するのかという疑問に新しい考え方を提供する.III.光干渉断層血管撮影(OCTA)による中心窩構造の解析中心窩形成・発達の過程で網膜内層は遠心性に移動して中心窩陥凹と中心窩無血管域(FAZ)が形成される.非侵襲的に網膜毛細血管を評価できるOCTAはOCT断層像のvolume scanを行っていることから,FAZ評価と同時に中心窩の形態変化を詳細に観察できる.正常眼でもFAZがなく網膜内層が残存している例があることが分かり,これは軽度の先天中心窩低形成である.未熟児網膜症(ROP)既往眼でも,FAZの縮小・消失と,中心窩での網膜内層の残存があり,FAZ面積は在胎週数と相関していた.ROP既往眼では,中心窩が未発達な状態が小児期にも持続していた.FAZが極端に小さな例を含めて全眼で,中心窩視細胞層の中心点(foveal bulge)はFAZ内に位置しており,FAZ形成と視細胞層の発達には密接な位置関係があることが分かった.IV.数理モデルによる中心窩形成過程の解明中心窩形成に関しては,少数例のヒト剖検眼とサル眼での研究が進められてきており,未だ不明な点が多い.そこで,ヒト網膜のFAZ形成と中心窩陥凹の発生に関して,数理モデルを用いてin silicoで形態形成過程を再現することで,メカニズムの解明を目指している.ヒト網膜血管パターンの数理モデルでは,FAZを形成する網膜血管の発生過程が得られ,ROP眼でみられる周辺部の無血管領域や彎入が再現された.中心窩陥凹形成モデルでは中央に弾力性のある部位を設け,水平方向と垂直方向の力を加えることで,OCT画像に類似した陥凹形態が得られた.これらのモデルの疾患シミュレーションへの応用を進めている.黄斑部に形態変化が起こりやすいのは,中心窩網膜に弾力性があることが関係している可能性がある.(著者抄録)

  • Fractality of Cranial Sutures

    Takashi Miura

    Advances in Neurobiology   36   227 - 240   2024   ISSN:2190-5215 eISSN:2190-5223

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    It has long been known that skull suture has a typical fractal structure. Although the fractal dimension has been utilized to assess morphology, the mechanism of the fractal structure formation remains to be elucidated. Recent advances in the mathematical modeling of biological pattern formation provided useful frameworks for understanding this mechanism. This chapter describes how various proposed mechanisms tried to explain the formation of fractal structures in cranial sutures.

    DOI: 10.1007/978-3-031-47606-8_11

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  • Mesoscale regulatory mechanisms of branch formation generating hierarchical structure of the lung

    Hisako Takigawa-Imamura, Hiroaki Takesue, Takashi Miura

    2023.3

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    The lung airways are characterized by long and wide proximal branches and short and thin distal branches. In this study, we investigated the emergence of this hierarchical structure through experimental observations and computational models. We focused on the branch formation in the pseudoglandular stage and examined the response of mouse lung epithelium to fibroblast growth factor 10 (FGF10) by monitoring extracellular signal-regulated kinase (ERK) activity. The ERK activity increased depending on the epithelial tissue curvature. This curvature-dependent response decreased as the development progressed. Therefore, to understand how these epithelial changes affect branching morphology, we constructed a computational model of curvature-dependent epithelial growth. We demonstrated that branch length was controlled by the curvature dependence of growth that was consistent with the experimental observations and lung morphology. However, the branching of the thin branches is suppressed in this model, which is inconsistent with the fact that thin branches in the lung are short. Thus, we introduced branch formation by apical constriction, which was shown to be regulated by Wnt signaling in our previous studies. Mathematical analysis indicated that the effect of apical constriction is cell shape-dependent, suggesting that apical constriction ameliorates the branching of thin branches. Finally, we were able to provide clarity on the hierarchical branching structure through an integrated computational model of curvature-dependent growth and cell shape regulation. We proposed that curvature-dependent growth involving FGF and Wnt-mediated cell shape regulation coordinate to control the spatial scale and frequency of branch formation.

    DOI: 10.1101/2023.03.23.533381

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  • マイクロ流体デバイスを用いた血管網に対するペリサイトの影響の評価

    寺岡佑佳子, ERICKSON Scott, 梨本裕司, 新宅博文, 小寺秀俊, 横川隆司, 中益朗子, 三浦岳, 有馬勇一郎, 花田三四郎, 西山功一

    電気学会全国大会講演論文集(CD-ROM)   2018.3

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    マイクロ流体デバイスを用いた血管網に対するペリサイトの影響の評価

  • オンチップ血管網での剪断応力知覚による血管新生の評価

    寺岡佑佳子, 中山雅宗, 梨本裕司, 中益朗子, 花田三四郎, 有馬勇一郎, 鳥澤勇介, 新宅博文, 小寺秀俊, 西山功一, 三浦岳, 横川隆司

    バイオエンジニアリング講演会講演論文集(CD-ROM)   2017.12

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    オンチップ血管網での剪断応力知覚による血管新生の評価

  • 灌流可能な血管を有するオンチップ腫瘍モデルの創出

    梨本裕司, 寺岡佑佳子, 有馬勇一朗, 花田三四郎, 中益朗子, 鳥澤勇介, 小寺秀俊, 西山功一, 三浦岳, 横川隆司

    センサ・マイクロマシンと応用システムシンポジウム(CD-ROM)   2017.10

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    灌流可能な血管を有するオンチップ腫瘍モデルの創出

  • 3D血管網を用いた剪断応力の知覚と伝達による血管新生評価システム

    寺岡佑佳子, 中山雅宗, 梨本裕司, 中益朗子, 花田三四郎, 有馬勇一郎, 鳥澤勇介, 新宅博文, 小寺秀俊, 西山功一, 三浦岳, 横川隆司

    センサ・マイクロマシンと応用システムシンポジウム(CD-ROM)   2017.10

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    3D血管網を用いた剪断応力の知覚と伝達による血管新生評価システム

  • Remodeling in synthetic vascular network - experiment and modeling Reviewed

    Takashi Miura, Nobuyuki Futai, Daiki Sasaki, Yuji Nashimoto, Ryuji Yokokawa, Kimiko Yamamoto, Shin Koide

    MECHANISMS OF DEVELOPMENT   2017.7

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    Remodeling in synthetic vascular network - experiment and modeling

    DOI: 10.1016/j.mod.2017.04.059

  • Development of three-dimensional tumor model with a perfusable vasculature using a microfluidic device Reviewed

    Yuji Nashimoto, Yukako Teraoka, Yuichiro Arima, Akiko Nakamasu, Yu-suke Torisawa, Hidetoshi Kotera, Koichi Nishiyama, Takashi Miura, Ryuji Yokokawa

    MECHANISMS OF DEVELOPMENT   2017.7

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    Development of three-dimensional tumor model with a perfusable vasculature using a microfluidic device

    DOI: 10.1016/j.mod.2017.04.041

  • Reconstitutive analyses of impacts of pericytes and blood flow on angiogenic morphogenesis using a microfluidic device Reviewed

    Koichi Nishiyama, Yuichiro Arima, Shigetomo Fukuhara, Yoshimi Yamaguchi, Michie Uchikawa, Yuji Nashimoto, Akiko Nakamasu, Ryuji Yokokawa, Takashi Miura

    MECHANISMS OF DEVELOPMENT   2017.7

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    Reconstitutive analyses of impacts of pericytes and blood flow on angiogenic morphogenesis using a microfluidic device

    DOI: 10.1016/j.mod.2017.04.158

  • 第32回研究会優秀研究賞 血管新生現象を利用した組織 : マイクロ流路間の接続と灌流培養への取り組み

    梨本 裕司, 國田 樹, 中益 朗子, 鳥澤 勇介, 中山 雅宗, 今村(滝川) 寿子, 小寺 秀俊, 西山 功一, 三浦 岳, 横川 隆司

    化学とマイクロ・ナノシステム = Journal of the Society for Chemistry and Micro-Nano Systems : 化学とマイクロ・ナノシステム研究会誌   2016.3

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    Anastomosis between tissue models and microchannels using angiogenic sprouts for the perfusable culture system

  • 発生における細胞外シグナル因子の拡散ダイナミクス (生物現象に対するモデリングの数理)

    三浦 岳

    数理解析研究所講究録   2012.4

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  • 発生生物学におけるチューリング系の応用--四肢骨格のパターン形成を例として (特集 数理生物学)

    三浦 岳

    蛋白質核酸酵素   2005.12

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  • 発生生物学における Turing 系の応用 - 四肢骨格のパターン形成を例として

    三浦 岳

    蛋白質核酸酵素   2005.12

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    生物の発生段階において、自発的なパターン形成によって周期構造が生じる事がある。数理生物学の分野ではこの現象をモデル化する際に Turing の反応拡散系が広く使われているが、発生生物学での応用例はま だ少ない。我々は四肢の骨格のパターン形成に関して Turing 系を応用 する試みをしているが、これによって実験的に進歩した部分、理論的に 進歩した部分をそれぞれ紹介し、数理生物学との相互作用による利点や 将来の展望について論じる。

    Repository Public URL: http://hdl.handle.net/2324/4751312

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Professional Memberships

  • Japanese Society for Mathematical Biologists

    2004.7 - Present

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  • 生物物理学会

    2004.7 - Present

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  • 発生生物学会

    1996.4 - Present

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  • 解剖学会

    1996.4 - Present

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  • 発生生物学会

  • 解剖学会

  • 先天異常学会

  • Society for Developmental Biologists

  • 数理生物学会

  • Japanese Society for Mathematical Biologists

  • 生物物理学会

  • 先天異常学会

  • 発生生物学会

  • 解剖学会

  • 生化学会

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  • Screening of academic papers

    Role(s): Peer review

    2020

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    Number of peer-reviewed articles in foreign language journals:9

  • Screening of academic papers

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    2019

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    Number of peer-reviewed articles in foreign language journals:9

  • Screening of academic papers

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    2018

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    Number of peer-reviewed articles in foreign language journals:10

  • Screening of academic papers

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    2017

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    Number of peer-reviewed articles in foreign language journals:8

  • その他

    日本数理生物学会  ( Japan ) 2016.9

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  • Screening of academic papers

    Role(s): Peer review

    2016

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:7

  • Screening of academic papers

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    2015

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    Number of peer-reviewed articles in foreign language journals:11

  • Screening of academic papers

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    2014

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    Number of peer-reviewed articles in foreign language journals:8

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Research Projects

  • スケーリング則を持つ脊椎動物組織のパターン形成機構の数理モデル化と実験的検証

    Grant number:24K02036  2024 - 2027

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 植物オルガネラの統合的フェノーム解析技術の研究

    Grant number:16K18562  2016 - 2018

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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  • 網膜新生血管における内皮細胞ダイナミクスの解析

    Grant number:16K15737  2016 - 2017

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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  • 発生におけるマルチスケールの自発的パターン形成現象の数理の解明

    Grant number:15KT0018  2015 - 2018

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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  • 葉表皮細胞の人為的変形系を用いた細胞形態および細胞間信号伝達シミュレーション解析

    Grant number:15K14536  2015 - 2016

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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  • からだの外でかたちを育てる

    2014 - 2019

    CREST

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    Authorship:Principal investigator  Grant type:Contract research

  • 植物表皮細胞壁のジグソーパズル構造形成メカニズム

    Grant number:26520207  2014 - 2016

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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  • 自己組織化を利用したオンチップ血管モデルの開発―血管生理・病態の再現と理解

    Grant number:26670394  2014 - 2015

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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  • 発生に於けるパターン形成現象の数理モデル化

    2014

    上原記念財団研究推進特別奨励金

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  • RhoJによる内皮細胞運動と血管網パターン形成の制御機構

    Grant number:25293078  2013 - 2015

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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  • 形態形成理解のためのマルチスケールマウス初期胚培養デバイス

    Grant number:25600060  2013 - 2015

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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  • 肺上皮の集団運動のメカニズムとmorphogen gradient

    Grant number:25111713  2013 - 2014

    Japan Society for the Promotion of Science・Ministry of Education, Culture, Sports, Science and Technology  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas

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    Grant type:Scientific research funding

  • 被毛パターン変異ラットを用いた反応拡散モデル実証のための実験モデル系の創出

    Grant number:25640046  2013 - 2014

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • 血管網を作る - 内皮細胞の自己組織化現象の実験と理論による解明

    Grant number:25127708  2013 - 2014

    Japan Society for the Promotion of Science・Ministry of Education, Culture, Sports, Science and Technology  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas

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    Grant type:Scientific research funding

  • 発生に於けるパターン形成現象の 数理モデル化

    2013

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 細胞骨格の制御を介した細胞外情報処理機構の解明

    Grant number:24114007  2012 - 2016

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • 生物の形作りの数理的記述法の確立

    Grant number:23654036  2011 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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  • 肺の枝分かれ構造形成における細胞集団運動のメカニズムの解明

    Grant number:23111514  2011 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • 生理学と協働した数理科学による皮膚疾患機構の解明

    2010.4 - 2015.3

    北海道大学 

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    Authorship:Collaborating Investigator(s) (not designated on Grant-in-Aid) 

  • 発生過程の関節軟骨の力学特性とリハビリテーションの基礎的研究

    Grant number:22700532  2010 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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  • 頭蓋骨縫合線のパターン形成の数理モデル化とその実験的検証

    Grant number:22659035  2010 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • 前脳形態形成におけるシグナル分子の役割の解明

    Grant number:20590169  2008 - 2010

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • モルフォゲンの濃度勾配ロバストネス保証および分化運命決定における閾値の分子機構

    Grant number:20022020  2008 - 2009

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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  • 上皮組織のかたちづくりを理解する

    2007 - 2010

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 生物における構造形成と情報に関する数理的研究

    Grant number:19340023  2007 - 2010

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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  • 前脳形態形成分子機構の解明

    Grant number:18590168  2006 - 2007

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • 哺乳動物の発生過程における自発的パターン形成現象の数理モデル化とその実験的検証

    Grant number:17689009  2005 - 2007

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • 内耳原基の器官培養系を用いた内耳の形態形成機構と内耳奇形発生のメカニズムの解明

    Grant number:14657005  2002 - 2004

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • マウス胎児肢芽細胞の微少集積培養系におけるパターン形成メカニズムの解明

    Grant number:13770010  2001 - 2002

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • 胎児の肺原基における初期分枝パターンの数理モデル化とその実験的検証

    Grant number:12877003  2000 - 2001

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • 哺乳類の形態形成現象に関与する分子の三次元的定量とその数理モデル化の研究

    Grant number:97J03294  1998 - 1999

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for JSPS Fellows

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    Authorship:Principal investigator  Grant type:Scientific research funding

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Educational Activities

  • Cell Biology
    Human Anatomy
    Histology

Class subject

  • 発生生物学

    2023.10 - 2024.3   Second semester

  • 人体構造概論

    2023.4 - 2023.9   First semester

  • 細胞生物学

    2023.4 - 2023.9   First semester

  • 人体解剖学概論

    2023.4 - 2023.9   First semester

  • 組織学

    2023.4 - 2023.9   First semester

  • 発生生物学

    2022.10 - 2023.3   Second semester

  • 人体構造概論

    2022.4 - 2022.9   First semester

  • 細胞生物学

    2022.4 - 2022.9   First semester

  • 人体解剖学概論

    2022.4 - 2022.9   First semester

  • 組織学

    2022.4 - 2022.9   First semester

  • 発生生物学

    2021.10 - 2022.3   Second semester

  • 人体構造概論

    2021.4 - 2021.9   First semester

  • 細胞生物学

    2021.4 - 2021.9   First semester

  • 人体解剖学概論

    2021.4 - 2021.9   First semester

  • 組織学

    2021.4 - 2021.9   First semester

  • 発生生物学

    2020.10 - 2021.3   Second semester

  • 人体構造概論

    2020.4 - 2020.9   First semester

  • 組織学

    2020.4 - 2020.9   First semester

  • 人体解剖学概論

    2020.4 - 2020.9   First semester

  • 細胞生物学

    2020.4 - 2020.9   First semester

  • 発生生物学

    2019.10 - 2020.3   Second semester

  • 人体構造概論

    2019.4 - 2019.9   First semester

  • 組織学

    2019.4 - 2019.9   First semester

  • 人体解剖学概論

    2019.4 - 2019.9   First semester

  • 発生生物学

    2018.10 - 2019.3   Second semester

  • 人体構造概論

    2018.4 - 2018.9   First semester

  • 組織学

    2018.4 - 2018.9   First semester

  • 人体解剖学概論

    2018.4 - 2018.9   First semester

  • 生命の科学A

    2018.4 - 2018.6   Spring quarter

  • 発生生物学

    2017.10 - 2018.3   Second semester

  • 数理医学

    2017.10 - 2017.12   Fall quarter

  • 人体構造概論

    2017.4 - 2017.9   First semester

  • 組織学

    2017.4 - 2017.9   First semester

  • 人体解剖学概論

    2017.4 - 2017.9   First semester

  • 発生生物学

    2016.10 - 2017.3   Second semester

  • 人体解剖学概論

    2016.4 - 2016.9   First semester

  • 人体構造概論

    2016.4 - 2016.9   First semester

  • 組織学

    2016.4 - 2016.9   First semester

  • 発生生物学

    2015.10 - 2016.3   Second semester

  • 人体解剖学概論

    2015.4 - 2015.9   First semester

  • 人体構造概論

    2015.4 - 2015.9   First semester

  • 組織学

    2015.4 - 2015.9   First semester

  • 人体解剖学概論

    2014.4 - 2014.9   First semester

  • 人体構造概論

    2014.4 - 2014.9   First semester

  • 組織学

    2014.4 - 2014.9   First semester

  • 発生生物学

    2014.4 - 2014.9   First semester

  • 人体構造概論

    2013.10 - 2014.3   Second semester

  • 組織学

    2013.4 - 2013.9   First semester

  • 人体構造と機能I

    2013.4 - 2013.9   First semester

  • 人体構造学概論

    2013.4 - 2013.9   First semester

  • 発生生物学

    2013.4 - 2013.9   First semester

  • 組織学

    2013.4 - 2013.9   First semester

  • 人体解剖学概論

    2013.4 - 2013.9   First semester

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FD Participation

  • 2015.12   Role:Participation   Title:H27年度大学院FD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

Other educational activity and Special note

  • 2023  Coaching of Students' Association  弓道部

     詳細を見る

    顧問

  • 2023  Coaching of Students' Association  数理医学研究会

     詳細を見る

    顧問

  • 2022  Coaching of Students' Association  数理医学研究会

     詳細を見る

    顧問

  • 2022  Coaching of Students' Association  弓道部

     詳細を見る

    顧問

  • 2020  Coaching of Students' Association  弓道部

     詳細を見る

    部長

  • 2020  Coaching of Students' Association  数理医学研究会

     詳細を見る

    顧問

  • 2019  Coaching of Students' Association  弓道部

     詳細を見る

    部長

  • 2019  Coaching of Students' Association  数理医学研究会

     詳細を見る

    顧問

  • 2018  Coaching of Students' Association  数理医学研究会

     詳細を見る

    顧問

  • 2018  Coaching of Students' Association  弓道部

     詳細を見る

    部長

  • 2017  Coaching of Students' Association  弓道部

     詳細を見る

    部長

  • 2017  Coaching of Students' Association  数理医学研究会

     詳細を見る

    顧問

  • 2016  Coaching of Students' Association  弓道部

     詳細を見る

    部長

  • 2016  Coaching of Students' Association  数理医学研究会

     詳細を見る

    顧問

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Media Coverage

  • 九大、毛細血管網を手軽に作製 生体外で生物学的な機能

    2020.10

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    九大、毛細血管網を手軽に作製 生体外で生物学的な機能

  • 九州大、細胞が細胞を包む仕組みは何か?―血管における細胞の形作りに数学で迫る―

    2020.2

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    九州大、細胞が細胞を包む仕組みは何か?―血管における細胞の形作りに数学で迫る―

  • NHKスペシャル「人体」

    2017.10

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    NHKスペシャル「人体」

  • 臓器につなげる血管網の作製器具「マイクロ流体デバイス」開発 京大など共同チーム…再生医療技術で普及期待

    2017.6

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    臓器につなげる血管網の作製器具「マイクロ流体デバイス」開発 京大など共同チーム…再生医療技術で普及期待

  • 京大と九大と熊本大、培養組織の中に毛細血管状の管を通し栄養や酸素を供給できるマイクロ流体デバイスを開発

    2017.6

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    京大と九大と熊本大、培養組織の中に毛細血管状の管を通し栄養や酸素を供給できるマイクロ流体デバイスを開発

  • 世界初、植物細胞の形の謎を解明 鍵は頭蓋骨縫合線と数学 九州大学ほか

    2016.4

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    世界初、植物細胞の形の謎を解明 鍵は頭蓋骨縫合線と数学 九州大学ほか

  • Turing vs. Wolpert - 反応拡散系に寄るパターン形成の応用研究の系譜 Newspaper, magazine

    現代思想Vol40-14  2012.10

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    Turing vs. Wolpert - 反応拡散系に寄るパターン形成の応用研究の系譜

  • サイエンスZERO シリーズ細胞の世界1 体をつくる不思議な"波”~

    2011.4

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    サイエンスZERO シリーズ細胞の世界1 体をつくる不思議な"波”~

  • シリーズ細胞の世界(1)体を作る不思議な「波」 TV or radio program

    サイエンスゼロ  2011.4

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    シリーズ細胞の世界(1)体を作る不思議な「波」

  • 動物たちの模様を解く魔法の数式 天才数学者が残した遺産

    2004.2

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    動物たちの模様を解く魔法の数式 天才数学者が残した遺産

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Travel Abroad

  • 2011.12

    Staying countory name 1:Spain   Staying institution name 1:Univ Compultense de Madrid

    Staying countory name 2:Germany   Staying institution name 2:EMBL Heidelberg

  • 2002.7 - 2004.6

    Staying countory name 1:United Kingdom   Staying institution name 1:Oxford University

    Staying countory name 2:Germany   Staying institution name 2:Heidelberg University