Updated on 2025/07/14

Information

 

写真a

 
SAKAI YASUNARI
 
Organization
Faculty of Medical Sciences Department of Clinical Medicine Associate Professor
Graduate School of Medical Sciences Department of Medical Sciences(Concurrent)
Graduate School of Medical Sciences Department of Medicine(Concurrent)
School of Medicine Department of Medicine(Concurrent)
Title
Associate Professor
Profile
1. Research: Molecular mechanisms of neuro-developmental disorders. 2. Education: Scientific training for graduate and undergraduate students 3. Clinical and social activity: Child neurology & Emergency service
External link

Research Areas

  • Life Science / System genome science

  • Life Science / Genome biology

  • Life Science / Medical biochemistry

  • Life Science / Embryonic medicine and pediatrics

Degree

  • M.D., Ph.D.

Research History

  • 九州大学大学院 医学研究院 成長発達医学分野(小児科学) Associate Professor 

    2017.4

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  • 九州大学病院 小児科 診療准教授 

    2014.4

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  • 九州大学病院 小児科 Lecturer 

    2011.4

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  • Kyushu University  Research Assistant 

    2005

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Research Interests・Research Keywords

  • Research theme: 修飾オリゴヌクレオチド

    Keyword: 修飾オリゴヌクレオチド

    Research period: 2025

  • Research theme: Protein complex

    Keyword: Protein complex

    Research period: 2025

  • Research theme: スプライシング

    Keyword: スプライシング

    Research period: 2025

  • Research theme: Interactome

    Keyword: Interactome

    Research period: 2025

  • Research theme: SHANK3

    Keyword: SHANK3

    Research period: 2025

  • Research theme: 多発性硬化症

    Keyword: 多発性硬化症

    Research period: 2025

  • Research theme: 急性散在性脳脊髄炎

    Keyword: 急性散在性脳脊髄炎

    Research period: 2025

  • Research theme: 急性脳炎

    Keyword: 急性脳炎

    Research period: 2025

  • Research theme: 細胞遺伝子療法

    Keyword: 細胞遺伝子療法

    Research period: 2025

  • Research theme: 結核菌

    Keyword: 結核菌

    Research period: 2025

  • Research theme: 脳機能

    Keyword: 脳機能

    Research period: 2025

  • Research theme: Autism

    Keyword: Autism

    Research period: 2025

  • Research theme: Language system

    Keyword: Language system

    Research period: 2025

  • Research theme: 遺伝子修復

    Keyword: 遺伝子修復

    Research period: 2025

  • Research theme: 遺伝子多型

    Keyword: 遺伝子多型

    Research period: 2025

  • Research theme: Systemic diseases in childhood and human-specific development of the postnatal brain function

    Keyword: Autism spectrum disorder, Epilepsy, Brain-Immune interaction, Interactome, Alternative splicing, Molecular signaling

    Research period: 2011.4

Awards

  • 第19回小児医学川野賞

    2019.3   川野小児医学奨学財団   小児脳疾患の収束的分子シグナルに関する研究

Papers

  • Heterogeneity and mitochondrial vulnerability configurate the divergent immunoreactivity of human induced microglia-like cells. Reviewed International journal

    Kousuke Yonemoto, Fumihiko Fujii, Ryoji Taira, Masahiro Ohgidani, Katsuhide Eguchi, Sayaka Okuzono, Yuko Ichimiya, Yuri Sonoda, Pin Fee Chong, Hironori Goto, Hikaru Kanemasa, Yoshitomo Motomura, Masataka Ishimura, Yuhki Koga, Keita Tsujimura, Takao Hashiguchi, Hiroyuki Torisu, Ryutaro Kira, Takahiro A Kato, Yasunari Sakai, Shouichi Ohga

    Clin Immunol   255   109756 - 109756   2023.10   ISSN:1521-6616 eISSN:1521-7035

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Clinical Immunology  

    Microglia play versatile roles in progression of and protection against neuroinflammatory diseases. Little is known, however, about the mechanisms underlying the diverse reactivity of microglia to inflammatory conditions. We investigated how human induced microglia-like (iMG) cells respond to innate immune ligands. Quantitative PCR showed that poly-I:C and lipopolysaccharide (LPS) activated the expression of IL1B and TNF. Immunoreactivity of iMG did not differ between controls (n = 11) and patients with neuroinflammatory diseases (n = 24). Flow cytometry revealed that CD14high cells expressed interleukin (IL) -1β after LPS treatment. Immunoblotting showed that poly-I:C and LPS differentially activated inflammatory pathways but commonly induced mitochondrial instability and the expression of pyruvate kinase isoform M2 (PKM2). Furthermore, a potent stimulator of PKM2 (DASA-58) alleviated IL-1β production after LPS treatment. These data indicate that heterogeneous cell populations and mitochondrial stability underlie the divergent immunoreactivity of human iMG in environments.

    DOI: 10.1016/j.clim.2023.109756

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  • Divergent neurodevelopmental profiles of very-low-birth-weight infants. Reviewed International journal

    Reina Ogata, Kyoko Watanabe, Pin Fee Chong, Jun Okamoto, Yoshihiro Sakemi, Toshinori Nakashima, Takuro Ohno, Hiroyuki Nomiyama, Yuri Sonoda, Yuko Ichimiya, Hirosuke Inoue, Masayuki Ochiai, Hironori Yamashita, Yasunari Sakai, Shouichi Ohga

    Pediatr Res   95 ( 1 )   233 - 240   2023.8   ISSN:0031-3998 eISSN:1530-0447

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Pediatric Research  

    BACKGROUND: Advanced perinatal medicine has decreased the mortality rate of preterm infants. Long-term neurodevelopmental outcomes of very-low-birth-weight infants (VLBWIs) remain to be investigated. METHODS: Participants were 124 VLBWIs who had in-hospital birth from 2007 to 2015. Perinatal information, developmental or intelligence quotient (DQ/IQ), and neurological comorbidities at ages 3 and 6 years were analyzed. RESULTS: Fifty-eight (47%) VLBWIs received neurodevelopmental assessments at ages 3 and 6 years. Among them, 15 (26%) showed DQ/IQ <75 at age 6 years. From age 3 to 6 years, 21 (36%) patients showed a decrease (≤-10), while 5 (9%) showed an increase (≥+10) in DQ/IQ scores. Eight (17%) with autism spectrum disorder or attention-deficit hyperactivity disorder (ASD/ADHD) showed split courses of DQ/IQ, including two with ≤-10 and one with +31 to their scores. On the other hand, all 7 VLBWIs with cerebral palsy showed DQ ≤35 at these ages. Magnetic resonance imaging detected severe brain lesions in 7 (47%) of those with DQ <75 and 1 (18%) with ASD/ADHD. CONCLUSIONS: VLBWIs show a broad spectrum of neurodevelopmental outcomes after 6 years. These divergent profiles also indicate that different risks contribute to the development of ASD/ADHD from those of cerebral palsy and epilepsy in VLBWIs. IMPACT: Very-low-birth-weight infants (VLBWIs) show divergent neurodevelopmental outcomes from age 3 to 6 years. A deep longitudinal study depicts the dynamic change in neurodevelopmental profiles of VLBWIs from age 3 to 6 years. Perinatal brain injury is associated with developmental delay, cerebral palsy and epilepsy, but not with ASD or ADHD at age 6 years.

    DOI: 10.1038/s41390-023-02778-w

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  • Long-lasting pain and somatosensory disturbances in children with myelin oligodendrocyte glycoprotein antibody-associated disease. Reviewed International journal

    Yuko Ichimiya, Pin Fee Chong, Yuri Sonoda, Vlad Tocan, Mitsuru Watanabe, Hiroyuki Torisu, Ryutaro Kira, Toshiyuki Takahashi, Jun-Ichi Kira, Noriko Isobe, Yasunari Sakai, Shouichi Ohga

    Eur J Pediatrics   182 ( 7 )   3175 - 3185   2023.4   ISSN:0340-6199 eISSN:1432-1076

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:European Journal of Pediatrics  

    UNLABELLED: Myelin oligodendrocyte glycoprotein antibody (MOG-Ab) is an autoantibody associated with acquired demyelinating syndrome (ADS) in childhood and adults. The pathogenic roles of MOG-Ab and long-term outcomes of children with MOG-Ab-associated disease (MOGAD) remain elusive. We investigated the clinical features of children with ADS during follow-up in our institute. Clinical data were retrospectively analyzed using medical charts of patients managed in Kyushu University Hospital from January 1st, 2001, to March 31st, 2022. Participants were children of < 18 years of age when they received a diagnosis of ADS in our hospital. Cell-based assays were used to detect MOG-Ab in serum or cerebrospinal fluid at the onset or recurrence of ADS. The clinical and neuroimaging data of MOG-Ab-positive and MOG-Ab-negative patients were statistically analyzed. Among 31 patients enrolled in this study, 22 (13 females, 59%) received tests for MOG antibodies. Thirteen cases (59%) were MOG-Ab-positive and were therefore defined as MOGAD; 9 (41%) were MOG-Ab-negative. There were no differences between MOGAD and MOG-Ab-negative patients in age at onset, sex, diagnostic subcategories, or duration of follow-up. MOGAD patients experienced headache and/or somatosensory symptoms more frequently than MOG-Ab-negative patients (12/13 (92%) vs. 3/9 (22%); p = 0.0066). Somatosensory problems included persistent pain with hyperesthesia in the left toe, perineal dysesthesia, and facial hypesthesia. No specific neuroimaging findings were associated with MOGAD or the presence of somatosensory symptoms. CONCLUSIONS: Long-lasting somatosensory disturbances are prominent comorbidities in children with MOGAD. Prospective cohorts are required to identify molecular and immunogenetic profiles associated with somatosensory problems in MOGAD. WHAT IS KNOWN: • Recurrence of demyelinating events occurs in a group of children with myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD). WHAT IS NEW: • Long-lasting headache and somatosensory problems are frequent comorbidities with pediatric MOGAD. Pain and somatosensory problems may persist for more than 5 years. • Neuroimaging data do not indicate specific findings in children with somatic disturbances.

    DOI: 10.1007/s00431-023-04989-z

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  • Shank3a/b isoforms regulate the susceptibility to seizures and thalamocortical development in the early postnatal period of mice. Reviewed International journal

    Sayaka Okuzono, Fumihiko Fujii, Yuki Matsushita, Daiki Setoyama, Yohei Shinmyo, Ryoji Taira, Kousuke Yonemoto, Satoshi Akamine, Yoshitomo Motomura, Masafumi Sanefuji, Takeshi Sakurai, Hiroshi Kawasaki, Kihoon Han, Takahiro A Kato, Hiroyuki Torisu, Dongchon Kang, Yusaku Nakabeppu, Yasunari Sakai, Shouichi Ohga

    Neurosci Res   193   13 - 19   2023.3   ISSN:0168-0102 eISSN:1872-8111

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Neuroscience Research  

    Epileptic seizures are distinct but frequent comorbidities in children with autism spectrum disorder (ASD). The hyperexcitability of cortical and subcortical neurons appears to be involved in both phenotypes. However, little information is available concerning which genes are involved and how they regulate the excitability of the thalamocortical network. In this study, we investigate whether an ASD-associated gene, SH3 and multiple ankyrin repeat domains 3 (Shank3), plays a unique role in the postnatal development of thalamocortical neurons. We herein report that Shank3a/b, the splicing isoforms of mouse Shank3, were uniquely expressed in the thalamic nuclei, peaking from two to four weeks after birth. Shank3a/b-knockout mice showed lower parvalbumin signals in the thalamic nuclei. Consistently, Shank3a/b-knockout mice were more susceptible to generalized seizures than wild-type mice after kainic acid treatments. Together, these data indicate that NT-Ank domain of Shank3a/b regulates molecular pathways that protect thalamocortical neurons from hyperexcitability during the early postnatal period of mice.

    DOI: 10.1016/j.neures.2023.03.001

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  • Neurodevelopmental Outcomes of High-Risk Preterm Infants A Prospective Study in Japan Reviewed International journal

    Michiko Torio, Mariko Iwayama, Toru Sawano, Hirosuke Inoue, Masayuki Ochiai, Ryoji Taira, Kousuke Yonemoto, Yuko Ichimiya, Yuri Sonoda, Momoko Sasazuki, Yoshito Ishizaki, Masafumi Sanefuji, Kenichi Yamane, Hiroshi Yamashita, Hiroyuki Torisu, Ryutaro Kira, Toshiro Hara, Shigenobu Kanba, Yasunari Sakai, Shouichi Ohga

    Neurol Clin Pract   11 ( 5 )   398 - 405   2021.10

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    DOI: 10.1212/CPJ.0000000000000920

  • Lipidomics links oxidized phosphatidylcholines and coronary arteritis in Kawasaki disease. Reviewed International journal

    Yasutaka Nakashima, Yasunari Sakai, Yumi Mizuno, Kenji Furuno, Keiichi Hirono, Shinichi Takatsuki, Hiroyuki Suzuki, Yoshihiro Onouchi, Tohru Kobayashi, Kazuhiro Tanabe, Kenji Hamase, Tomofumi Miyamoto, Ryohei Aoyagi, Makoto Arita, Kenichiro Yamamura, Tamami Tanaka, Hisanori Nishio, Hidetoshi Takada, Shouichi Ohga, Toshiro Hara

    Cardiovasc Res   117 ( 1 )   96 - 108   2021.1

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    DOI: 10.1093/cvr/cvz305

  • GNAO1 organizes the cytoskeletal remodeling and firing of developing neurons. Reviewed International journal

    Satoshi Akamine, Sayaka Okuzono, Hiroyuki Yamamoto, Daiki Setoyama, Noriaki Sagata, Masahiro Ohgidani, Takahiro A Kato, Tohru Ishitani, Hiroki Kato, Keiji Masuda, Yuki Matsushita, Hiroaki Ono, Yoshito Ishizaki, Masafumi Sanefuji, Hirotomo Saitsu, Naomichi Matsumoto, Dongchon Kang, Shigenobu Kanba, Yusaku Nakabeppu, Yasunari Sakai, Shouichi Ohga

    FASEB J   34 ( 12 )   16601 - 16621   2020.12

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    DOI: 10.1096/fj.202001113R

  • De Novo Truncating Mutation of TRIM8 Causes Early-Onset Epileptic Encephalopathy. Reviewed International journal

    Sakai Y, Fukai R, Matsushita Y, Miyake N, Saitsu H, Akamine S, Torio M, Sasazuki M, Ishizaki Y, Sanefuji M, Torisu H, Shaw CA, Matsumoto N, Hara T

    Ann Hum Genet   80 ( 4 )   235 - 240   2016.7

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    De Novo Truncating Mutation of TRIM8 Causes Early-Onset Epileptic Encephalopathy.
    BACKGROUND: Early-onset epileptic encephalopathy (EOEE) is a heterogeneous group of neurodevelopmental disorders characterised by infantile-onset intractable epilepsy and unfavourable developmental outcomes. Hundreds of mutations have been reported to cause EOEE; however, little is known about the clinical features of individuals with rare variants. CASE REPORT AND METHODS: We present a 10-year-old boy with severe developmental delay. He started experiencing recurrent focal seizures at 2 months old. Serial electroencephalograms persistently detected epileptiform discharges from the left hemisphere. Whole-exome sequencing and array-comparative genome hybridization were performed to search for de novo variations. Two-week-old C57Bl/6 mice were used for immunofluorescence studies. RESULTS: This case had a paternally inherited, 0.2-Mb duplication at chromosome 22q11.22. The whole-exome sequencing identified a de novo truncating mutation of tripartite motif containing 8 (TRIM8) (NM_030912:c.1099_1100insG:p.C367fs), one of the epileptic encephalopathy-associated genes. We verified that the murine homologues of these genes are expressed in the postnatal mouse brain. CONCLUSION: This is the second case of EOEE caused by a de novo truncating mutation of TRIM8. Further studies are required to determine the functional roles of TRIM8 in the postnatal development of the human brain and its functional relationships with other EOEE-associated genes.

    DOI: 10.1111/ahg.12157

  • Hyperactive mTOR signals in the proopiomelanocortin-expressing hippocampal neurons cause age-dependent epilepsy and premature death in mice. Reviewed International journal

    Matsushita Y, Sakai Y, Shimmura M, Shigeto H, Nishio M, Akamine S, Sanefuji M, Ishizaki Y, Torisu H, Nakabeppu Y, Suzuki A, Takada H, Hara T

    Sci Rep   6   22991 - 22991   2016.3

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    Hyperactive mTOR signals in the proopiomelanocortin-expressing hippocampal neurons cause age-dependent epilepsy and premature death in mice.
    Epilepsy is a frequent comorbidity in patients with focal cortical dysplasia (FCD). Recent studies utilizing massive sequencing data identified subsets of genes that are associated with epilepsy and FCD. AKT and mTOR-related signals have been recently implicated in the pathogenic processes of epilepsy and FCD. To clarify the functional roles of the AKT-mTOR pathway in the hippocampal neurons, we generated conditional knockout mice harboring the deletion of Pten (Pten-cKO) in Proopiomelanocortin-expressing neurons. The Pten-cKO mice developed normally until 8 weeks of age, then presented generalized seizures at 8-10 weeks of age. Video-monitored electroencephalograms detected paroxysmal discharges emerging from the cerebral cortex and hippocampus. These mice showed progressive hypertrophy of the dentate gyrus (DG) with increased expressions of excitatory synaptic markers (Psd95, Shank3 and Homer). In contrast, the expression of inhibitory neurons (Gad67) was decreased at 6-8 weeks of age. Immunofluorescence studies revealed the abnormal sprouting of mossy fibers in the DG of the Pten-cKO mice prior to the onset of seizures. The treatment of these mice with an mTOR inhibitor rapamycin successfully prevented the development of seizures and reversed these molecular phenotypes. These data indicate that the mTOR pathway regulates hippocampal excitability in the postnatal brain.

    DOI: 10.1038/srep22991

  • Protein Interactome Reveals Converging Molecular Pathways Among Autism Disorders Reviewed International journal

    Yasunari Sakai, Chad A. Shaw, Brian C. Dawson, Diana V. Dugas, Zaina Al-Mohtaseb, David E. Hill, Huda Y. Zoghbi

    Sci Transl Med   3 ( 86 )   86ra49   2011.6

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    DOI: 10.1126/scitranslmed.3002166

  • A molecular basis for the selective recognition of 2-hydroxy-dATP and 8-oxo-dGTP by human MTH1. Reviewed International journal

    Yasunari Sakai, Masato Furuichi, Masayuki Takahashi, Masaki Mishima, Shigenori Iwai, Masahiro Shirakawa, Yusaku Nakabeppu

    J Biol Chem   277 ( 10 )   8579 - 87   2002.3

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    DOI: 10.1074/jbc.M110566200

  • National study on pediatric acute encephalopathy in Japan (April 2020 to October 2023): Insights from the third study

    Omata T., Sakuma H., Nagase H., Abe Y., Kuki I., Okumura A., Maegaki Y., Murayama K., Sakai Y., Hoshino A., Mizuguchi M., Takanashi J.i.

    Brain and Development   47 ( 4 )   104387   2025.8   ISSN:03877604

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    Language:English   Publisher:Brain and Development  

    Background: Previous national studies of acute encephalopathy in Japan were conducted in 2007–2010 and 2014–2017. In this third study (April 1, 2020–October 31, 2023), spanning the coronavirus disease 2019 (COVID-19) outbreak, we compared results to assess trends in pediatric viral infections and therapeutic practices. Methods: Questionnaires were sent to 430 hospitals of the Japanese Pediatric Society, yielding 241 responses and 1197 cases. A secondary survey to 151 facilities received 110 responses (72.8 %), identifying 622 eligible patients (604 for treatment, 544 for outcome analysis). Data on patient background, syndrome classification, causative virus, treatment, and prognosis were collected. Results: Among 622 cases (54.6 % boys), the highest incidence was in 1-year-olds, with case numbers peaking during COVID-19 and influenza outbreaks. The frequency of clinical syndromes was: acute encephalopathy with biphasic seizures and late reduced diffusion, 35.1 %; clinically mild encephalitis/encephalopathy with a reversible splenial lesion, 17.8 %; hemorrhagic shock and encephalopathy syndrome (HSES), 6.9 %; acute encephalitis with refractory, repetitive partial seizures, 3.7 %; acute fulminant cerebral edema (AFCE), 2.2 %; acute necrotizing encephalopathy, 1.6 %; and unclassifiable, 31.4 %. Notably, the combined HSES/AFCE rate increased from 1.9 % in the 2014–2017 survey to 9.1 %, likely due to enhanced diagnosis and COVID-19 impact. Pathogens were exanthem subitum (16.1 %), severe acute respiratory syndrome coronavirus 2 (15.9 %), and influenza (7.7 %). Treatments included steroid pulse therapy (68.9 %), mitochondrial cocktails (42.5 %), and targeted temperature management (31.0 %). Conclusion: This study provides a comprehensive overview of pediatric acute encephalopathy during COVID-19 outbreaks, shows increases in the incidence of HSES/AFCE, and presents current treatment.

    DOI: 10.1016/j.braindev.2025.104387

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  • Total corpus callosotomy for an adult patient with progressive myoclonic epilepsy associated with dentatorubral-pallidoluysian atrophy: illustrative case.

    Mine D, Shimogawa T, Sakai Y, Shigeto H, Okubo S, Sakata A, Watanabe E, Nakamizo A, Yoshimoto K

    Journal of neurosurgery. Case lessons   10 ( 1 )   2025.7

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    DOI: 10.3171/CASE2576

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  • Larotrectinib Monotherapy After a Subtotal Resection of Infantile Hemispheric Glioma With TPM3::NTRK1 Fusion. International journal

    Satomi Yokoyama, Utako Oba, Toya Shunichiro, Yoshinao Oda, Koji Yoshimoto, Yasunari Sakai, Yuhki Koga, Shouichi Ohga

    Pediatric blood & cancer   72 ( 7 )   e31694   2025.7   ISSN:1545-5009 eISSN:1545-5017

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    DOI: 10.1002/pbc.31694

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  • Standardizing the Definition of Each Colon Cancer Segment: Delphi Consensus on Clinical Decision-Making for Oncologic Outcomes

    Kuzu M.A., Benlice C., Parvaiz A., Gorgun E., Bertelsen C.A., Wexner S.D., Dozois E.J., Hohenberger W., Miskovic D., Sugihara K., Spinelli A., Wiggers T., Lee W.Y., Möslein G., Tsarkov P., Basany E.E., Patrón Uriburu J.C., Perez R.O., Lynch C., Liu Z., Hahnloser D., Nilsson P.J., Chowdri N.A., Brown G., Rouanet P., Madoff R.D., West N.P., Sahin T., Elhan A.H., Bordeianou L.G., Abedrapo M., Adamina M., Aguilar J.G., Aigner F., Aiupov R., Akyol C., Aly E.H., Amorim E., Andersen P.V., Ando K., Araujo S.E.A., Asplund D., Atallah S., Avellaneda N., Azevedo J., Baca B., Bai J., Balaban V., Baral J., Bardakcioglu O., Behm K., Belgers H.J., Benecke C., Benno S., Benz S., Bergamaschi R., Berger D.L., Bianchi P.P., Biondo S., Boni L., Bugra D., Bundgaard L., Byrne C., Carvello M., Chand M., Chew M.H., Choen F.S., Christoph I., Chung S.S., Cirocco W.C., Cohan J., Consten E.C.J., Cotte E., Coyne P., Crolla R., Croner R., Cunha M., D'Hoore A., Dacanal F.M., Daneri M.D., Davids J.S., Davies J., de Manzini N., de Wilt J.H.W., de Beche-Adams T., Dekker J.W., Delaney C.P., Delrio P., Dennett E., Dutra Vila R.D., Dimitri C., Dzhumabaev K., Elliot A.H., Eray İ.C., Erygin D., Evans C., Faes S., Faucheron J.L., Feoktistov D., Fernandez L.M.

    Diseases of the Colon and Rectum   68 ( 7 )   835 - 844   2025.7   ISSN:00123706

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    BACKGROUND: Data registries lack a definitive classification system that distinguishes different locations of colon cancer from one another. OBJECTIVE: To establish an international consensus on the definition of primary colon cancer segment locations. DESIGN: Between December 2022 and June 2023, the Delphi survey study was conducted to seek opinions from relevant international experts and eventually develop a consensus definition of each colon cancer segment. SETTING: Three-round online-based Delphi survey study. INTERVENTIONS: The online survey included 17 questions. In the first 2 rounds, participating experts were asked to rank each statement on a scale of 1 (least relevant) to 9 (most relevant). Consensus statements and definitions were revised according to the results for statements obtaining a consensus score of 7 to 9. During the third round and online meeting, definitions and statements that reached a moderate or high consensus (above 4 for more than 70% of participants) were included. MAIN OUTCOME MEASURES: The primary goal of our project was focused on precisely localizing the specific segment affected by primary colon cancer rather than identifying surgical treatment or type of resection needed for a particular segment. RESULTS: The first round included 331 experts; 301 (91%) completed the second round and 295 (98%) completed the final round. Experts strongly supported the use of a “10-cm rule” to describe colon cancer sites at the flexures and anatomical landmarks for other segments. Regarding the definition of rectosigmoid cancer, experts from United States and Europe reached a high consensus that the term rectosigmoid as a colon cancer location must be abolished in contrast to experts from Asia. The description of overlapping segments of cancers achieved a consensus of 64%. LIMITATIONS: Subjective decisions are based on individual expert clinical experience. CONCLUSIONS: This Delphi survey, the first internationally conducted consensus study, achieved a remarkable level of consensus among a panel of global experts. Ambiguity still exists regarding overlapping lesions. See Video Abstract.

    DOI: 10.1097/DCR.0000000000003739

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  • Spontaneous histiocytic sarcoma with peritoneal dissemination in a Damaraland mole-rat (<i>Fukomys damarensis</i>): The first spontaneous tumor in this species

    Sakai, Y; Sekiguchi, K; Suzuki, Y; Kobe, M; Oka, K; Yamakawa, M; Kawamura, Y; Buffenstein, R; Miura, K

    VETERINARY PATHOLOGY   3009858251349124   2025.6   ISSN:0300-9858 eISSN:1544-2217

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  • Temporal changes in the urologist's practice behaviour of active surveillance for prostate cancer: analysis of prospective observational study cohort

    Tohi, Y; Yokomizo, A; Matsumoto, R; Mori, K; Sakamoto, S; Shiota, M; Sekine, Y; Kanno, T; Kato, T; Fukuhara, H; Sakai, Y; Kohjimoto, Y; Matsuda, I; Goto, T; Kawamura, N; Kusuhara, Y; Hashine, K; Tsumura, H; Naito, Y; Sugimoto, M

    JAPANESE JOURNAL OF CLINICAL ONCOLOGY   2025.6   ISSN:0368-2811 eISSN:1465-3621

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  • Anti-Oxidized Low-Density Lipoprotein Antibodies Before and After Intravenous Immunoglobulin Therapy in Kawasaki Disease ― Evidence for a Potentially Protective Role ―

    Kano Zenpei, Mizuno Yumi, Murata Kenji, Onoyama Sagano, Hoshina Takayuki, Sakai Yasunari, Kishimoto Junji, Kusuhara Koichi, Hara Toshiro

    Circulation Reports   7 ( 5 )   359 - 364   2025.5   eISSN:24340790

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    Language:English   Publisher:The Japanese Circulation Society  

    <p><b><i>Background:</i></b> The precise pathogenesis of Kawasaki disease (KD) remains unclear, but immune dysregulation involving damage-associated molecular patterns (DAMPs), such as oxidized low-density lipoprotein (LDL) and high mobility group box 1 (HMGB1), has been implicated. We investigated the roles of 2 anti-DAMPs antibodies in KD and their associations with inflammatory and oxidative stress markers.</p><p><b><i>Methods and Results:</i></b> Serum levels of anti-oxidized LDL and anti-HMGB1 antibodies were measured by enzyme-linked immunosorbent assay in patients with KD and in febrile disease controls (DC). Correlations with inflammatory (C-reactive protein [CRP]) and oxidative stress (red blood cell distribution width [RDW]) markers were evaluated. Serum anti-oxidized LDL antibody levels increased significantly after intravenous immunoglobulin (IVIG) therapy in KD patients, suggesting a protective role of anti-oxidized LDL antibodies against vascular inflammation. Conversely, anti-HMGB1 antibody levels showed a decreasing trend post-IVIG. A significant correlation between antibody levels and CRP was observed in DC but not in KD patients. Furthermore, a weak inverse trend between anti-oxidized LDL antibodies and RDW-coefficient of variation was noted in KD patients.</p><p><b><i>Conclusions:</i></b> This study highlighted the distinct roles of anti-oxidized LDL and anti-HMGB1 antibodies during the acute phase of KD. The increase in anti-oxidized LDL antibodies following IVIG treatment suggests a protective effect, while the transient nature of anti-HMGB1 antibodies warrants further exploration.</p>

    DOI: 10.1253/circrep.cr-25-0018

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  • Active Surveillance in Prostate Cancer With Intermediate-Risk Features: The PRIAS-JAPAN Study

    Blas, L; Shiota, M; Kato, T; Matsumoto, R; Tohi, Y; Sakamoto, S; Yokomizo, A; Kimura, T; Furukawa, J; Shoji, S; Kume, H; Goto, T; Sekine, Y; Sakai, Y; Matsuoka, Y; Hinata, N; Kamoto, T; Terada, N; Akamatsu, S; Sugimoto, M; Eto, M

    INTERNATIONAL JOURNAL OF UROLOGY   32 ( 7 )   823 - 827   2025.4   ISSN:0919-8172 eISSN:1442-2042

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    Objectives: To report outcomes of active surveillance (AS) for prostate cancer in men with intermediate-risk features of International Society of Urological Pathology (ISUP) grade group 2 and/or clinical stage T2 compared with ISUP grade group 1 and clinical stage T1 in the PRIAS-JAPAN study. Methods: Patients with prostate cancer diagnosed between January 2010 and February 2024 were included in this study. PSA test, rectal examination, and re-biopsy were performed regularly. We calculated the pathological reclassification rate, program persistence rate, and subsequent treatment. Results: Data from 1302 participants were collected. After excluding patients who did not fit inclusion criteria (n = 28) or follow-up of less than 1 year (n = 208), 1066 patients were included in this analysis. The median follow-up was 42.4 months (interquartile range 17.0–72.1). There were no statistical differences in the pathological reclassification, persistence rates, and subsequent therapy between low- and intermediate-risk features. Conclusion: This preliminary study demonstrated medium-term outcomes of AS in prostate cancer with intermediate-risk features in Japan, suggesting no significant difference in the pathological reclassification, persistence rate, and subsequent therapy between low- and intermediate-risk features.

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  • Neurodevelopmental Changes and Postnatal Growth in the First 3 Years of Extremely Preterm Infants

    Matsunaga, Y; Inoue, H; Miyauchi, Y; Watabe, T; Yasuoka, K; Sawano, T; Ochiai, M; Sakai, Y; Ohga, S

    NEONATOLOGY   122 ( 2 )   181 - 190   2025.4   ISSN:1661-7800 eISSN:1661-7819

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    Introduction: Infants born extremely preterm are at high risk for neurodevelopmental problems. However, their neurodevelopment exhibits a variety of trajectories. This study aimed to investigate the association between changes in neurodevelopmental outcomes and clinical characteristics among extremely preterm infants. Methods: This is a retrospective study of surviving children born at gestational age 22–28 weeks in Kyushu University Hospital between 2010 and 2020. We collected perinatal and post-discharge data and investigated the association between clinical characteristics and changes in developmental quotient (DQ) scores between 1.5 and 3 years of corrected age. Results: Out of the 179 eligible extremely preterm infants, 115 (64%) underwent neurological evaluations at 1.5 and 3 years of corrected age. Among them, 33 (29%) showed improvement in their DQ scores (+10 or more), 62 (54%) showed no change (−9 to +9), and 20 (17%) showed a decline (−10 or less). Gestational age, birth weight, and perinatal complications during the NICU stay did not affect individual changes in DQ scores. Multivariable analysis revealed that greater growth in height until age 3 years was a significant predictor of increasing DQ scores, while male sex and having siblings had a negative effect on changes in the DQ scores. Conclusion: We first demonstrate clinical data conceptualizing that growth in height, sex, and sibling status, rather than perinatal complications, are biologically linked with favorable or unfavorable neurodevelopmental changes of extremely preterm infants during the first 3 years of life.

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  • Methylmalonic acidemia with recurrent hemophagocytic lymphohistiocytosis: a case report and review of the literature

    Yamashita, F; Akamine, S; Chong, PF; Maeda, K; Kawakami, S; Lee, S; Ishimura, M; Murayama, K; Sakai, Y; Kira, R

    BMC PEDIATRICS   25 ( 1 )   259   2025.3   eISSN:1471-2431

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    Background: Methylmalonic acidemia is a rare autosomal recessive disorder of propionate catabolism characterized by the accumulation of propionic acid and methylmalonic acid caused by methylmalonyl-CoA mutase deficiency. Clinical presentations range from acute deterioration in the neonatal period to later onset with a heterogeneous clinical course. Metabolite accumulation results in systemic involvement, affecting the nervous, gastrointestinal, and renal system functions and causing cardiomyopathy. Bone marrow dysfunction manifesting as neutropenia and anemia is a common hematological finding. Although rare, three cases of secondary hemophagocytosis were documented. Case presentation: An 18-year-old male patient diagnosed with methylmalonic acidemia presented with vomiting and altered mental status. He had a medical history of presumably hemophagocytic lymphohistiocytosis (HLH) at the age of 17 months. Physical examination, laboratory tests, and bone marrow aspiration results met the HLH-2004 diagnostic criteria, confirming a recurrent HLH. Although he recovered after intensive treatment, his cognitive function declined. Retrospective analysis revealed higher serum levels of ferritin during acute decompensations compared with nonattack periods. Correlation analysis revealed a strong relationship between serum ferritin and propionylcarnitine, one of the major propionyl-CoA-derived metabolites. Conclusions: HLH is a rare and underrecognized hematologic emergency in methylmalonic acidemia, and its early diagnosis and treatment are critical. Serum ferritin may be a useful clinical biomarker in the diagnosis of HLH-associated attacks in methylmalonic acidemia.

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  • Aconserved human CD4+Tcell subset recognizing the mycobacterial adjuvanttrehalose monomycolate

    Sakai, Y; Asa, M; Hirose, M; Kusuhara, W; Fujiwara, N; Tamashima, H; Ikazaki, T; Oka, S; Kuraba, K; Tanaka, K; Yoshiyama, T; Nagae, M; Hoshino, Y; Motooka, D; Van Rhijn, I; Lu, XY; Ishikawa, E; Moody, DB; Kato, T; Inuki, S; Hirai, G; Yamasaki, S

    JOURNAL OF CLINICAL INVESTIGATION   135 ( 6 )   2025.3   ISSN:0021-9738 eISSN:1558-8238

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    Mycobacterium tuberculosis causes human tuberculosis (TB). As mycobacteria are protected by a thick lipid cell wall, humans have developed immune responses against diverse mycobacterial lipids. Most of these immunostimulatory lipids are known as adjuvants acting through innate immune receptors, such as C-type lectin receptors. Although a few mycobacterial lipid antigens activate unconventional T cells, the antigenicity of most adjuvantic lipids is unknown. Here, we identified that trehalose monomycolate (TMM), an abundant mycobacterial adjuvant, activated human T cells bearing a unique αβ T cell receptor (αβTCR). This recognition was restricted by CD1b, a monomorphic antigen-presenting molecule conserved in primates but not mice. Single-cell TCR-RNA-Seq using newly established CD1b-TMM tetramers revealed that TMM-specific T cells were present as CD4+ effector memory T cells in the periphery of uninfected donors but expressed IFN-γ, TNF, and anti-mycobacterial effectors upon TMM stimulation. TMM-specific T cells were detected in cord blood and PBMCs of donors without bacillus Calmette-Guérin vaccination but were expanded in patients with active TB. A cryo-electron microscopy study of CD1b-TMM-TCR complexes revealed unique antigen recognition by conserved features of TCRs, positively charged CDR3α, and long CDR3β regions. These results indicate that humans have a commonly shared and preformed CD4+ T cell subset recognizing a typical mycobacterial adjuvant as an antigen. Furthermore, the dual role of TMM justifies reconsideration of the mechanism of action of adjuvants.

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  • Genome Analysis of Japanese Yersinia pseudotuberculosis Strains Isolated From Kawasaki Disease Patients and Other Sources and Their Phylogenetic Positions in the Global Y.pseudotuberculosis Population(タイトル和訳中)

    Yasuoka Kazuaki, Gotoh Yasuhiro, Taniguchi Itsuki, Nagano Debora Satie, Nakamura Keiji, Mizuno Yumi, Abe Tomoko, Ogura Yoshitoshi, Nakajima Hiroshi, Uesugi Masayoshi, Miura Masaru, Seto Kazuko, Wakabayashi Yuki, Isobe Junko, Watari Takashi, Senda Sonoko, Hayakawa Noboru, Ogawa Eiki, Sato Toshio, Nanishi Etsuro, Sakai Yasunari, Kato Atsushi, Miyata Ippei, Ouchi Kazunobu, Ohga Shouichi, Hara Toshiro, Hayashi Tetsuya

    Microbiology and Immunology   69 ( 3 )   182 of 190 - 190 of 190   2025.3   ISSN:0385-5600

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  • Genome Analysis of Japanese <i>Yersinia pseudotuberculosis</i> Strains Isolated From Kawasaki Disease Patients and Other Sources and Their Phylogenetic Positions in the Global <i>Y. pseudotuberculosis</i> Population

    Yasuoka, K; Gotoh, Y; Taniguchi, I; Nagano, DS; Nakamura, K; Mizuno, Y; Abe, T; Ogura, Y; Nakajima, H; Uesugi, M; Miura, M; Seto, K; Wakabayashi, Y; Isobe, J; Watari, T; Senda, S; Hayakawa, N; Ogawa, E; Sato, T; Nanishi, E; Sakai, Y; Kato, A; Miyata, I; Ouchi, K; Ohga, S; Hara, T; Hayashi, T

    MICROBIOLOGY AND IMMUNOLOGY   69 ( 3 )   182 - 190   2025.3   ISSN:0385-5600 eISSN:1348-0421

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    Yersinia pseudotuberculosis (Ypt) is a gram-negative bacterium that infects both humans and animals primarily through fecal‒oral transmission. While Ypt causes acute gastroenteritis in humans, an association with Kawasaki disease (KD), a disease that primarily affects infants and young children and causes multisystemic vasculitis, has also been suspected. Although KD represents a significant health concern worldwide, the highest annual incidence rate is reported in Japan. Previously, a geographical origin-dependent population structure of Ypt comprising the Asian, transitional, and European clades was proposed. However, genomic data on KD-associated Ypt strains is currently unavailable. In this study, to analyze the phylogenetic and genomic features of KD-associated strains, we determined the whole-genome sequences of 35 Japanese Ypt strains, including 11 KD-associated strains, and constructed a genome set (n = 204) representing the global population of Ypt by adding publicly available Ypt genomes. In a phylogenetic analysis, all sequenced Japanese strains, including the KD-associated strains, belonged to the Asian clade, which appeared to be the ancestral clade of Ypt, and the KD-associated strains belonged to multiple lineages in this clade. Strains from patients with Far East scarlet-like fever (FESLF), a KD-related disease, also belonged to the Asian clade. Moreover, no KD strain-specific genes were identified in pan-genome-wide association study analyses. Notably, however, the gene encoding a superantigen called Yersinia pseudotuberculosis-derived mitogen (YPM) showed a distribution pattern highly biased to the Asian clade. Although further studies are needed, our results suggest that Asian clade strains may have a greater potential to trigger KD.

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  • Access Program for Unapproved and Off-Label Drug Use in Pediatric <i>BRAF</i> V600E-Mutated Brain Tumors in Japan

    Suzuki, M; Koga, Y; Kawasaki, T; Ueda, T; Yamamoto, S; Goto, H; Kishimoto, J; Ishida, E; Todaka, K; Sonoda, KH; Oda, Y; Koji, Y; Sakai, Y; Ohga, S

    PEDIATRIC BLOOD & CANCER   72 ( 3 )   e31510   2025.3   ISSN:1545-5009 eISSN:1545-5017

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    Programs allowing access to investigational drugs and off-label drug use for serious diseases have often been applied to pediatric cancers. A clinical study conducted under the Japanese “Patient-Proposed Healthcare Services” evaluated the efficacy and safety of dabrafenib plus trametinib in children with BRAF V600 mutant glioma (jRCTs071210071). This study successfully provided unapproved and off-label medications to four enrolled patients, two with low-grade glioma and two with high-grade glioma (median age: 10.5 years), until regulatory approval. The timeframe and data collection from such access programs need to be optimized for pediatric patients in accordance with the healthcare system of each nation.

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  • Expanding diversity in developmental profiles of very-low-birth-weight infants during 6 years after birth

    Watanabe, K; Ogata, R; Kajiwara, K; Inoue, H; Sakemi, Y; Ichiyama, M; Sawano, T; Yasuoka, K; Watabe, T; Kurata, H; Nakashima, T; Sonoda, Y; Chong, PF; Akamine, S; Ochiai, M; Ohno, T; Yamashita, H; Sakai, Y; Ohga, S

    SCIENTIFIC REPORTS   15 ( 1 )   4504   2025.2   ISSN:2045-2322

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    Very-low-birth-weight infants (VLBWIs) are at high risk for neurodevelopmental problems after age 3 years. We investigated the association between the developmental quotient (DQ) of VLBWIs and their growth profiles during 6 years after birth. Participants were VLBWIs born at Kokura Medical Center (the first cohort) and Kyushu University Hospital (the second cohort) between 2012 and 2017. Recorded charts were used to collect growth profiles and developmental quotients (DQ) of the participants until age 6 years. In the first cohort (n = 64), the DQ values at age 6 years were correlated with those at age 3 years. VLBWIs with DQ ≥ 85 at age 6 years had a higher body weight and height at age 3 years than those with DQ < 85. The second cohort (n = 69) validated these findings. A comparative analysis of the two cohorts revealed that the DQ of VLBWIs was dissociated from their growth profiles after age 3 years. Clustering analyses indicated that DQ values at age 3 years predicted better the prognosis of VLBWIs with DQ ≥ 85 at age 6 years than their growth profiles. This study demonstrates that VLBWIs gain divergent profiles in growth, development, and growth-and-development correlation during postnatal 6 years.

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  • Green Surgery Awareness and Challenges: A Survey Among Members of the Japan Society for Endoscopic Surgery

    Ikenaga, N; Nakamura, M; Hirasawa, M; Naitoh, T; Sakai, Y; Habuchi, T; Kitagawa, Y

    ASIAN JOURNAL OF ENDOSCOPIC SURGERY   18 ( 1 )   e70087   2025.1   ISSN:1758-5902 eISSN:1758-5910

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    Introduction: Global warming poses an urgent challenge, with the healthcare sector being a significant contributor to greenhouse gas (GHG) emissions. The rise in minimally invasive surgery, which often depends on energy-intensive technologies and single-use devices, further exacerbates this environmental burden. The concept of “Green Surgery” aims to reduce the environmental footprint while maintaining patient care standards. Yet awareness and attitudes of surgeons in Japan regarding these practices are largely unknown. Methods: The Japanese Society for Endoscopic Surgery (JSES)'s Working Group on Environmental Issues conducted an online survey with 21 questions exploring knowledge of Green Surgery and perspectives on sustainable practices. The survey was distributed to all JSES members, with anonymous responses collected between October 18 and November 12, 2024. Results: A total of 1601 participants (10.1%) responded. Awareness of Green Surgery was limited, with only 5% demonstrating a well-developed understanding, though 67% expressed concerns about GHG emissions and operating room waste. Environmentally friendly practices were reported by only 14% of respondents' facilities, with perceived cost increases (55%) and workflow changes (39%) as key barriers. Nonetheless, 82% were willing to adopt sustainable practices, preferring reusable instruments (74%) and remanufactured single-use devices (66%). Conclusion: This survey highlights the challenges and opportunities in promoting Green Surgery in Japan. Despite limited awareness, there is considerable interest in adopting sustainable practices. These findings support the JSES in spearheading initiatives to integrate sustainability into surgical practices, thereby aiding in achieving global climate goals.

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  • 一過性の意識減損を呈し定型欠神発作と鑑別を要したhyperventilation-induced high-amplitude rhythmic slowing with altered awareness(HIHARSAA)の1例

    野田 麻里絵, 一宮 優子, 奥園 清香, 酒井 康成, 鳥巣 浩幸

    脳と発達   57 ( 1 )   45 - 48   2025.1   ISSN:0029-0831

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    Hyperventilation-induced high-amplitude rhythmic slowing with altered awareness(HIHARSAA)は,過呼吸負荷時に脳波上で高振幅かつ律動的な徐波化を生じ,意識変容を呈する現象であり,年齢依存性の非てんかん性生理現象と考えられている.今回我々は,脳波ビデオ同時記録検査でHIHARSAAと診断した4歳女児例を経験したので報告する.症例は,啼泣後の過呼吸時に一過性の意識減損を認め,精査目的に当院を受診した.来院時,意識清明で神経学的異常所見を認めず,頭部MRI・MRAで脳動脈主幹部の狭窄などの有意な異常所見を認めなかった.脳波検査では,発作間欠期に異常を認めなかったが,過呼吸負荷時に律動的な3~3.5Hz全般性高振幅徐波が突発的に出現し,脳波異常が出現する間,意識が朦朧となり,口をもぐもぐさせ,手を動かし,そわそわと落ち着かない様子を認めた.発作症状は定型欠神発作との鑑別が困難であったが,発作時脳波が棘波を伴わない律動性高振幅徐波であったことからHIHARSAAと診断し,無投薬で経過観察とした.HIHARSAAは稀な現象であるが,定型欠神発作と類似しており,両者の鑑別には脳波ビデオ同時記録が有用と考えられる.(著者抄録)

  • Towards pediatric neurological care that leaves no one behind : Are there regional disparities? Utilizing online medical consultations

    Akasaka Manami, Okazaki Shin, Nozaki Takuro, Motojima Toshino, Sakai Yasunari, Takahashi Satoru, Hirata Yuko, Kaga Yoshimi

    NO TO HATTATSU   57 ( 2 )   91 - 101   2025   ISSN:00290831 eISSN:18847668

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  • A case of hyperventilation-induced high-amplitude rhythmic slowing with altered awareness requiring differentiation from typical absence seizure

    Noda Marie, Ichimiya Yuko, Okuzono Sayaka, Sakai Yasunari, Torisu Hiroyuki

    NO TO HATTATSU   57 ( 1 )   45 - 48   2025   ISSN:00290831 eISSN:18847668

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    <p>  Hyperventilation-induced high-amplitude rhythmic slowing with altered awareness (HIHARSAA) is an age-dependent nonepileptic physiological phenomenon induced by hyperventilation, in which rhythmic high-amplitude slow-wave activity appears on electroencephalogram (EEG) and transiently alters the consciousness. Here, we report the case of a 4-year-old girl who was diagnosed with HIHARSAA by EEG video recording. She showed transient loss of consciousness during hyperventilation followed by crying and was subsequently admitted to our hospital for detailed examination. She was alert and had no neurological abnormalities. Head MRI and MRA showed no significant abnormal findings such as stenosis of the main cerebral arteries. Electroencephalography showed no abnormalities during the interictal period. During hyperventilation, a sudden occurrence of rhythmic high-amplitude 3-3.5 Hz slow-wave activity was observed on EEG, and she became dazed, started to mumble, moved her hands, and began to fidget, consistent with the appearance of EEG abnormalities. Although it was difficult to differentiate the seizures from typical absence seizures, the patient was diagnosed with HIHARSAA because the ictal EEG was a rhythmic high-amplitude slow wave without spikes. Hence, she was followed up without medication. Although HIHARSAA is a rare phenomenon, it should be differentiated from typical absence seizure by means of EEG video recording.</p>

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  • Improvement of image quality of dentomaxillofacial region in ultra-high-resolution CT: a phantom study

    Sakai, Y; Okamura, K; Kitamoto, E; Shirasaka, T; Kato, T; Chikui, T; Ishigami, K

    DENTOMAXILLOFACIAL RADIOLOGY   54 ( 3 )   203 - 209   2024.12   ISSN:0250-832X eISSN:1476-542X

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    Objectives: The purpose of this study was to compare the image quality of ultra-high-resolution CT (U-HRCT) with that of conventional multidetector row CT (convCT) and demonstrate its usefulness in the dentomaxillofacial region. Methods: Phantoms were helically scanned with U-HRCT and convCT scanners using clinical protocols. In U-HRCT, phantoms were scanned in super-high-resolution (SHR) mode, and hybrid iterative reconstruction (HIR) and filtered-back projection (FBP) techniques were performed using a bone kernel (FC81). The FBP technique was performed using the same kernel as in convCT (reference). Two observers independently evaluated the 54 resulting images using a 5-point scale (5 = excellent diagnostic image quality; 4 = above average; 3 = average; 2 = subdiagnostic; and 1 = unacceptable). The system performance function (SPF) was calculated for a comprehensive evaluation of the image quality using the task transfer function and noise power spectrum. Statistical analysis using the Kruskal-Wallis test was performed to compare the image quality among the 3 protocols. Results: The observers assigned higher scores to images acquired with the SHRHIR and SHRFBP protocols than to those acquired with the reference (P < 0.0001 and P < 0.0001, respectively). The relative SPF value at 1.0 cycles/mm in SHRHIR and SHRFBP compared to the reference protocol were 151.5% and 45.6%, respectively. Conclusions: Through phantom experiments, this study demonstrated that U-HRCT can provide superior-quality images compared to conventional CT in the dentomaxillofacial region. The development of a better image reconstruction method is required to improve image quality and optimize the radiation dose.

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  • Diagnostic MR imaging features of hypomyelination of early myelinating structures: A case report

    Abe, T; Yamashita, K; Kikuchi, K; Hatai, E; Fujii, F; Chong, PF; Sakai, Y; Saitsu, H; Inoue, K; Togao, O; Ishigami, K

    NEURORADIOLOGY JOURNAL   37 ( 6 )   758 - 760   2024.12   ISSN:1971-4009 eISSN:2385-1996

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    Hypomyelination of early myelinating structures (HEMS) has recently been defined as a new genetic disorder accompanied by clinical and MR imaging characteristics. However, no studies have focused on diffusion-weighted imaging (DWI) findings of HEMS. We would like to propose a “sheep sign,” which is formed by DWI hyperintensity in the medial medullary lamina along with alternating high-low-high (HLH) intensity stripes in the posterior limb of the internal capsule. We believe the presence of the “sheep sign” on DWI in combination with alternating HLH intensity stripes may be a valuable tool for diagnosing HEMS.

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  • Prevalence of, and Disability Due to, Multiple Sclerosis and Neuromyelitis Optica Spectrum Disorder in Japan by the Fifth Nationwide Survey

    Mitsuru Watanabe, Noriko Isobe, Masaaki Niino, Ichiro Nakashima, Takuya Matsushita, Yasunari Sakai, Jin Nakahara, Izumi Kawachi, Hirofumi Ochi, Yuji Nakatsuji, Yosikazu Nakamura, Koshi Nakamura, Kiyomi Sakata, Makoto Matsui, Satoshi Kuwabara, Jun-ichi Kira

    Neurology   103 ( 10 )   e209992   2024.11   ISSN:0028-3878 eISSN:1526-632X

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    BACKGROUND AND OBJECTIVES: All 4 previous nationwide surveys of multiple sclerosis (MS) in Japan were conducted before the discovery of anti-aquaporin-4 (AQP4) antibodies; thus, neuromyelitis optica spectrum disorder (NMOSD) was included in MS, as optic-spinal MS. We aimed to clarify the epidemiologic features and trends of MS and NMOSD in Japan separately using a fifth nationwide survey. METHODS: The primary survey, in which a questionnaire was sent to 3,799 selected departments (including neurology/internal medicine, pediatrics, and ophthalmology), explored the estimated number and prevalence of patients with MS or NMOSD in 2017, and the secondary survey collected detailed characteristics of the patients using a second questionnaire. RESULTS: The response rates for the primary and secondary surveys were 60.1% and 53.9%, respectively. The estimated total number of patients with MS or NMOSD was 24,800, 2.5-fold higher than that in the fourth survey in 2003. The crude prevalence was 19.6 per 100,000 patients (14.2 for MS and 5.4 for NMOSD), compared with 7.7 per 100,000 patients in the fourth survey. Patients with MS showed milder disability (median Expanded Disability Status Scale [EDSS] score: 2.0 [interquartile range 1.0-4.5] vs 2.5 [1.0-6.0]), decreased secondary progression (8.5% vs 15.2%), and increased usage of disease-modifying drugs (63.7% vs 37.2%) compared with those with conventional MS in the fourth survey. The proportions of oligoclonal bands and Barkhof criteria fulfillment on MRI, which are features of classical MS, increased with advancing year of birth. Patients with NMOSD also showed less disability and shorter disease duration than patients with optic-spinal MS in the fourth survey (EDSS score: 3.5 [2.0-5.5] vs 3.8 [2.0-6.0]; disease duration: 8.0 [3.9-14.8] vs 10.0 [5.0-16.0]). Among patients with NMOSD, disability was exacerbated by a history of longitudinally extensive spinal cord lesions and anti-AQP4 antibody positivity, which both decreased with advancing year of birth. DISCUSSION: The prevalences of MS (particularly with classical features) and NMOSD have been increasing in Japan, suggesting the contribution of environmental factors. However, disabilities in patients with MS and NMOSD have been mitigated. Extensive usage of various disease-modifying drugs could be a factor contributing to this disability mitigation in MS.

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  • Dural arteriovenous fistulae in a 6-year-old girl with trisomy 21 and congenital heart disease. International journal

    Toshiya Ishikura, Yuri Sonoda, Kenta Kajiwara, Pin Fee Chong, Hikaru Kanemasa, Yoshitomo Motomura, Noriyuki Kaku, Yuichiro Hirata, Hazumu Nagata, Kenichiro Yamamura, Koichi Arimura, Akira Nakamizo, Yasunari Sakai, Shouichi Ohga

    Clinical neurology and neurosurgery   246   108540 - 108540   2024.11   ISSN:0303-8467 eISSN:1872-6968

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    Dural arteriovenous fistula (DAVF) represents a pathological group of intracranial shunts arising from the dural artery to venous sinus and veins. Childhood-onset DAVF is generally considered to be poor in prognosis, whereas only limited information is available for the onset and long-term outcomes. We herein report a Japanese girl with trisomy 21, large ventricular septal defects, and pulmonary vein stenosis, for which a transcatheter stent had been placed after birth. At age 6 years, she developed bacterial meningitis due to S. pneumoniae, leading to the diagnosis of venous sinus thrombosis and multiple intracranial shunts. Cerebral angiography identified multiple shunts arising from the middle meningeal arteries to the superior sagittal sinus and a concurrent reflux to cortical vein. Endovascular embolization successfully occluded the shunts without neurovascular complications over 24 months. This report first demonstrates the favorable outcome of DAVF in a pediatric patient with trisomy 21 after the catheter intervention. For children at a risk for intracranial thrombosis, preemptive neurovascular evaluation and transcatheter intervention provide a chance of early diagnosis of DAVF to improve their survival and neurologic outcome.

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  • 後天性眼振を示したHypomyelination of Early Myelinating Structure(HEMS)の2歳男児

    畑井 恵理子, チョン・ピンフィー, 安部 時子, 梶原 健太, 藤井 史彦, 園田 有里, 赤峰 哲, 菊地 一史, 山下 孝二, 栂尾 理, 石神 康生, 才津 浩智, 井上 健, 酒井 康成, 大賀 正一

    日本小児科学会雑誌   128 ( 10 )   1365 - 1365   2024.10   ISSN:0001-6543

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  • Practice guidelines on endoscopic surgery for qualified surgeons by the endoscopic surgical skill qualification system: Large intestine

    Kuroyanagi, H; Hida, K; Ishii, Y; Yamamoto, S; Hasegawa, S; Takahashi, K; Saida, Y; Inomata, M; Nakamura, M; Sakai, Y

    ASIAN JOURNAL OF ENDOSCOPIC SURGERY   17 ( 4 )   e13364   2024.10   ISSN:1758-5902 eISSN:1758-5910

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  • Respiratory viral infections and Kawasaki disease: A molecular epidemiological analysis. International journal

    Kentaro Marutani, Kenji Murata, Yumi Mizuno, Sagano Onoyama, Takayuki Hoshina, Kenichiro Yamamura, Kenji Furuno, Yasunari Sakai, Junji Kishimoto, Koichi Kusuhura, Toshiro Hara

    Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi   57 ( 5 )   691 - 699   2024.10   ISSN:1684-1182 eISSN:1995-9133

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    BACKGROUND/PURPOSE: Recent large-scale epidemiological studies have revealed significant temporal associations between certain viral infections and the subsequent development of Kawasaki disease (KD). Despite these associations, definitive laboratory evidence linking acute or recent viral infections to KD cases remains elusive. The objective of this study is to employ a molecular epidemiological approach to investigate the temporal association between viral infections and the development of KD. METHODS: We analyzed 2460 patients who underwent the FilmArray® Respiratory Panel test between April 2020 and September 2021. RESULTS: Following the application of inclusion criteria, 2402 patients were categorized into KD (n = 148), respiratory tract infection (n = 1524), and control groups (n = 730). The KD group exhibited higher positive rates for respiratory syncytial virus (RSV), human rhinovirus/enterovirus (hRV/EV), parainfluenza virus (PIV) 3, and adenovirus (AdV) compared to the control group. Additionally, coinfections involving two or more viruses were significantly more prevalent in the KD group. Notably, RSV-positive, hRV/EV-positive, and PIV3-positive KD patients exhibited a one-month delay in peak occurrence compared to non-KD patients positive for corresponding viruses. In contrast, AdV-positive KD cases did not show a one-month delay in peak occurrence. Moreover, anti-RSV, anti-PIV3, and anti-AdV antibody-positive rates or antibody titers were higher in RSV-, PIV3-, and AdV-positive KD cases, respectively, compared to non-KD cases with the same viral infections. CONCLUSION: Recent infection with RSV, PIV3, or AdV, occasionally in conjunction with other viruses, may contribute to the pathogenesis of KD as infrequent complications.

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  • An N‐terminal and ankyrin repeat domain interactome of Shank3 identifies the protein complex with the splicing regulator Nono in mice

    Sayaka Okuzono, Fumihiko Fujii, Daiki Setoyama, Ryoji Taira, Yohei Shinmyo, Hiroki Kato, Keiji Masuda, Kousuke Yonemoto, Satoshi Akamine, Yuki Matsushita, Yoshitomo Motomura, Takeshi Sakurai, Hiroshi Kawasaki, Kihoon Han, Takahiro A. Kato, Hiroyuki Torisu, Dongchon Kang, Yusaku Nakabeppu, Shouichi Ohga, Yasunari Sakai

    Genes to Cells   29 ( 9 )   746 - 756   2024.9   ISSN:1356-9597 eISSN:1365-2443

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    An autism‐associated gene Shank3 encodes multiple splicing isoforms, Shank3a‐f. We have recently reported that Shank3a/b‐knockout mice were more susceptible to kainic acid‐induced seizures than wild‐type mice at 4 weeks of age. Little is known, however, about how the N‐terminal and ankyrin repeat domains (NT‐Ank) of Shank3a/b regulate multiple molecular signals in the developing brain. To explore the functional roles of Shank3a/b, we performed a mass spectrometry‐based proteomic search for proteins interacting with GFP‐tagged NT‐Ank. In this study, NT‐Ank was predicted to form a variety of complexes with a total of 348 proteins, in which RNA‐binding (n = 102), spliceosome (n = 22), and ribosome‐associated molecules (n = 9) were significantly enriched. Among them, an X‐linked intellectual disability‐associated protein, Nono, was identified as a NT‐Ank‐binding protein. Coimmunoprecipitation assays validated the interaction of Shank3 with Nono in the mouse brain. In agreement with these data, the thalamus of Shank3a/b‐knockout mice aberrantly expressed splicing isoforms of autism‐associated genes, Nrxn1 and Eif4G1, before and after seizures with kainic acid treatment. These data indicate that Shank3 interacts with multiple RNA‐binding proteins in the postnatal brain, thereby regulating the homeostatic expression of splicing isoforms for autism‐associated genes after birth.

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  • 【子どもの心と育ちを理解するツール】小児科医の目からみた遠城寺式乳幼児分析的発達検査の有用性と課題

    市山 正子, 川上 沙織, 井上 普介, 吉良 龍太郎, 酒井 康成, 大賀 正一

    教育と医学   72 ( 5 )   418 - 426   2024.9   ISSN:0452-9677

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  • オナセムノゲンアベパルボベク投与後、RSウイルス感染をきっかけに急性肝不全を発症した脊髄性筋萎縮症I型乳児の1例(Acute liver failure worsened after respiratory syncytial virus infection in an infant with spinal muscular atrophy type I after receiving onasemnogene abeparvovec)

    Sakemi Shohei, Fujita Takako, Kaku Noriyuki, Yatsuga Shuichi, Ito Kazutoshi, Sasaoka Daiki, Yamaguchi Hiromi, Hayashi Hitomi, Inoue Takahito, Higashi Kanako, Sakai Yasunari, Ohga Shouichi, Nagamitsu Shinichiro

    Brain and Development Case Reports   2 ( 3 )   j.bdcasr.2024.100022 - j.bdcasr.2024.100022   2024.9

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    症例は生後2ヵ月の乳児で、生後1ヵ月で筋力低下を認め、遺伝子スクリーニングでSMN1エクソン7のホモ接合性欠失およびSMN2コピー数2を有し、脊髄性筋萎縮症(SMA)I型と診断された。抗AAV9抗体が陽性であったため、ヌシネルセンやオナセムノゲンアベパルボベク(OA)は使用せず、代替として経口リスジプラムを導入した。抗体価は徐々に低下し、生後7ヵ月で1:25未満となったため、OA(1.1×10^14 vector genome/kg)を投与した。投与前日より経口プレドニゾロン1mg/kg/日を開始した。OA投与5日後にAST 100U/L、ALT 39U/Lと軽度の肝酵素上昇を認めたが自然軽快した。投与46日後にRSウイルス感染による呼吸困難が出現し、51日後には人工呼吸管理が必要となった。52日後に急性肝不全を発症し、AST 24297U/L、ALT 6128U/L、LDH 21659U/L、凝固異常を認めた。ヒドロコルチゾン7mg/kg静注とメチルプレドニゾロン4mg/kg/日で加療し、呼吸状態と肝機能は改善した。

  • 特集 公費補助制度を使いこなす! Ⅱ.各論 多発性硬化症

    梶原 健太, チョン ピンフィー, 酒井 康成

    小児科診療   87 ( 8 )   1080 - 1084   2024.8   ISSN:03869806

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  • Multiple endocrine neoplasia type 2B diagnosed after small intestinal volvulus with progressive megacolon in an adolescent

    Sakai, Y; Nakayama, Y; Kurasawa, S; Sado, T; Kato, S; Hidaka, N; Takamizawa, S; Yoshizawa, K; Yoshimaru, K; Taguchi, T

    CLINICAL JOURNAL OF GASTROENTEROLOGY   17 ( 4 )   640 - 646   2024.8   ISSN:1865-7257 eISSN:1865-7265

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    Multiple endocrine neoplasia type 2B is a rare autosomal dominant disease characterized by the presence of medullary thyroid carcinoma, pheochromocytoma, Marfan-like fatigue, a peculiar face with thickening of the lips, mucosal neuromas on the lips and tongue, and gastrointestinal phenomena. Most patients harbor pathological variants of the RET gene. Herein, we present the first case of a 14 year-old boy who experienced small intestinal volvulus along with a megacolon, and he was diagnosed with multiple endocrine neoplasia type 2B. The patient complained of constipation since he was 2 years old and slowly progressive abdominal distension at school age. At 14 years of age, he presented with remarkable megacolon mimicking Hirschsprung’s disease and complicated with small intestinal volvulus. The volvulus was successfully repaired, and the particularly dilated transverse colon was resected following a rectal biopsy. Histopathological evaluation of the resected transverse colon revealed to be compatible with ganglioneuromatosis. After emergency surgery, the patient was diagnosed with multiple endocrine neoplasia type 2B with medullary thyroid carcinoma, and a de novo variant of RET was confirmed. Gastroenterologists should consider it when treating patients with constipation, especially those with megacolon. Therefore, timely diagnosis may lead to appropriate treatment of medullary thyroid carcinoma and improve mortality.

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  • Gnao1 is a molecular switch that regulates the Rho signaling pathway in differentiating neurons

    Ryoji Taira, Satoshi Akamine, Sayaka Okuzono, Fumihiko Fujii, Eriko Hatai, Kousuke Yonemoto, Ryuichi Takemoto, Hiroki Kato, Keiji Masuda, Takahiro A. Kato, Ryutaro Kira, Keita Tsujimura, Kenichiro Yamamura, Norio Ozaki, Shouichi Ohga, Yasunari Sakai

    Scientific Reports   14 ( 1 )   17097   2024.7   ISSN:2045-2322 eISSN:2045-2322

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    GNAO1 encodes G protein subunit alpha O1 (Gαo). Pathogenic variations in GNAO1 cause developmental delay, intractable seizures, and progressive involuntary movements from early infancy. Because the functional role of GNAO1 in the developing brain remains unclear, therapeutic strategies are still unestablished for patients presenting with GNAO1-associated encephalopathy. We herein report that siRNA-mediated depletion of Gnao1 perturbs the expression of transcripts associated with Rho GTPase signaling in Neuro2a cells. Consistently, siRNA treatment hampered neurite outgrowth and extension. Growth cone formation was markedly disrupted in monolayer neurons differentiated from iPSCs from a patient with a pathogenic variant of Gαo (p.G203R). This variant disabled neuro-spherical assembly, acquisition of the organized structure, and polarized signals of phospho-MLC2 in cortical organoids from the patient’s iPSCs. We confirmed that the Rho kinase inhibitor Y27632 restored these morphological phenotypes. Thus, Gαo determines the self-organizing process of the developing brain by regulating the Rho-associated pathway. These data suggest that Rho GTPase pathway might be an alternative target of therapy for patients with GNAO1-associated encephalopathy.

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  • The immunoreactive signature of monocyte-derived dendritic cells from patients with Down syndrome. International journal

    Kentaro Nakashima, Takashi Imai, Akira Shiraishi, Ryoko Unose, Hironori Goto, Yusaku Nagatomo, Kanako Kojima-Ishii, Yuichi Mushimoto, Kei Nishiyama, Kenichiro Yamamura, Hazumu Nagata, Masataka Ishimura, Koichi Kusuhara, Yuhki Koga, Yasunari Sakai, Shouichi Ohga

    Clinical and experimental immunology   217 ( 3 )   291 - 299   2024.6   ISSN:0009-9104 eISSN:1365-2249

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    The clinical spectrum of Down syndrome (DS) ranges from congenital malformations to premature aging and early-onset senescence. Excessive immunoreactivity and oxidative stress are thought to accelerate the pace of aging in DS patients; however, the immunological profile remains elusive. We investigated whether peripheral blood monocyte-derived dendritic cells (MoDCs) in DS patients respond to lipopolysaccharide (LPS) distinctly from non-DS control MoDCs. Eighteen DS patients (age 2-47 years, 12 males) and 22 controls (age 4-40 years, 15 males) were enrolled. CD14-positive monocytes were immunopurified and cultured for 7 days in the presence of granulocyte-macrophage colony-stimulating factor and IL-4, yielding MoDCs in vitro. After the LPS-stimulation for 48 hours from days 7 to 9, culture supernatant cytokines were measured by multiplex cytokine bead assays, and bulk-prepared RNA from the cells was used for transcriptomic analyses. MoDCs from DS patients produced cytokines/chemokines (IL-6, IL-8, TNF-α, MCP-1, and IP-10) at significantly higher levels than those from controls in response to LPS. RNA sequencing revealed that DS-derived MoDCs differentially expressed 137 genes (74 upregulated and 63 downregulated) compared with controls. A gene enrichment analysis identified 5 genes associated with Toll-like receptor signaling (KEGG: hsa04620, P = 0.00731) and oxidative phosphorylation (hsa00190, P = 0.0173) pathways. MoDCs obtained from DS patients showed higher cytokine or chemokine responses to LPS than did control MoDCs. Gene expression profiles suggest that hyperactive Toll-like receptor and mitochondrial oxidative phosphorylation pathways configure the immunoreactive signature of MoDCs in DS patients.

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  • ATP1A3 regulates protein synthesis for mitochondrial stability under heat stress. International journal

    Fumihiko Fujii, Hikaru Kanemasa, Sayaka Okuzono, Daiki Setoyama, Ryoji Taira, Kousuke Yonemoto, Yoshitomo Motomura, Hiroki Kato, Keiji Masuda, Takahiro A Kato, Shouichi Ohga, Yasunari Sakai

    Disease models & mechanisms   17 ( 6 )   2024.6   ISSN:1754-8403 eISSN:1754-8411

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    Pathogenic variants in ATP1A3, the α3 subunit of the Na+/K+-ATPase-encoding gene, cause alternating hemiplegia of childhood (AHC) and related disorders. Impairments in Na+/K+-ATPase activity are associated with the clinical phenotype. However, it remains unclear whether additional mechanisms are involved in the exaggerating symptoms under stressed conditions in patients with AHC. We herein report that the intracellular loop (ICL) of ATP1A3 interacted with RNA-binding proteins, such as EIF4G, PABPC1 and FMRP. Both the siRNA-mediated depletion of Atp1a3 and ectopic expression of the p.R756C-variant ATP1A3-ICL in Neuro2a cells resulted in excessive phosphorylation of ribosomal protein S6 and increased susceptibility to heat stress. In agreement with these findings, iPSCs from a patient with the p.R756C variant were more vulnerable to heat stress than control iPSCs. Neurons established from the patient's iPSCs showed lower calcium influxes in responses to stimulation with ATP than controls. These data indicated that inefficient protein synthesis contributes to the progressive and deteriorating phenotypes of patients with the p.R756C variant among a variety of ATP1A3-related disorders.

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  • 産院退院時には健康と判断された児が新生児期に発症した疾患 PICUを有する大学病院による疫学調査

    井上 普介, 藤吉 順子, 宮内 雄太, 渡部 貴秀, 安岡 和昭, 澤野 徹, 落合 正行, 大賀 正一, 虫本 雄一, 本村 良知, 西山 慶, 賀来 典之, 永田 弾, 山村 健一郎, 石村 匡崇, 古賀 友紀, 酒井 康成

    日本周産期・新生児医学会雑誌   60 ( Suppl.1 )   P282 - P282   2024.6   ISSN:1348-964X eISSN:2435-4996

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  • Mechanism of intermetallic charge transfer and bond disproportionation in BiNiO3 and PbNiO3 revealed by hard x-ray photoemission spectroscopy

    Yamaguchi, T; Furo, M; Sakai, Y; Nishikubo, T; Hojo, H; Azuma, M; Oka, K; Mori, D; Inaguma, Y; Mizumaki, M; Yamamoto, K; Kunes, J; Mizokawa, T; Hariki, A

    PHYSICAL REVIEW B   109 ( 20 )   2024.5   ISSN:2469-9950 eISSN:2469-9969

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    Perovskites with Bi or Pb on the A site host a number of interesting and yet to be understood phenomena such as negative thermal expansion in BiNiO3. We employ hard x-ray photoemission spectroscopy of Ni 2p core level as well as valence band to probe the electronic structure of BiNiO3 and PbNiO3. The experimental results supported by theoretical calculations using dynamical mean-field theory reveal essentially identical electronic structure of the Ni-O subsystem typical of Ni2+ charge-transfer insulators. The two materials are distinguished by filling of the Bi(Pb)-O antibonding states in the vicinity of the Fermi level, which is responsible for the Bi disproportionation in BiNiO3 at ambient pressure and absence of similar behavior in PbNiO3. The present experiments provide evidence for this conclusion by revealing the presence/absence of Bi/Pb 6s states at the top of the valence band in the two materials.

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  • 日本の地域治療ネットワークにおける家族性血球貪食性リンパ組織球症に対する早期造血細胞移植(Early hematopoietic cell transplantation for familial hemophagocytic lymphohistiocytosis in a regional treatment network in Japan)

    Ishimura Masataka, Eguchi Katsuhide, Sonoda Motoshi, Tanaka Tamami, Shiraishi Akira, Sakai Yasunari, Yasumi Takahiro, Miyamoto Takayuki, Voskoboinik Ilia, Hashimoto Kunio, Matsumoto Shirou, Ozono Shuichi, Moritake Hiroshi, Takada Hidetoshi, Ohga Shouichi

    International Journal of Hematology   119 ( 5 )   592 - 602   2024.5   ISSN:0925-5710

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    家族性血球貪食性リンパ組織球症(FHLH)に対する根治的造血細胞移植(HCT)を確立するため、九州の地域治療ネットワークにおける骨髄非破壊的造血幹細胞移植の治療成績を検討した。2011年から2022年までにフローサイトメトリースクリーニングにより診断されたFHLH患児13例(FHLH2;11例、FHLH3;2例)を対象とした。新規PRF1バリアントの1例はスクリーニングから漏れたが、FHLH2の全例が診断に至り、そのうち8例は生後3ヵ月と9ヵ月までにHCTを受けた。最も早いHCTは生後65日目に行われた。移植前の死亡例は診断時に肝不全であった新生児の3例であった。移植後の患児10例は無病状態を維持しており、そのうち7例には神経学的病変を認めなかった。最初のエトポシド点滴静注からHCTまでの期間は、中枢神経系疾患や出血のない患児の方が、それらの因子を有する患児よりも短かった。加えて、血縁関係のない患児9例中6例にPRF1 c.1090_1091delCTバリアントが認められた。

  • Early hematopoietic cell transplantation for familial hemophagocytic lymphohistiocytosis in a regional treatment network in Japan

    Ishimura M., Eguchi K., Sonoda M., Tanaka T., Shiraishi A., Sakai Y., Yasumi T., Miyamoto T., Voskoboinik I., Hashimoto K., Matsumoto S., Ozono S., Moritake H., Takada H., Ohga S.

    International Journal of Hematology   119 ( 5 )   592 - 602   2024.5   ISSN:09255710

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    Familial hemophagocytic lymphohistiocytosis (FHLH) is a fatal hyperinflammation syndrome arising from the genetic defect of perforin-mediated cytolysis. Curative hematopoietic cell transplantation (HCT) is needed before development of central nervous system (CNS) disease. We studied treatment outcomes of 13 patients (FHLH2 n = 11, FHLH3 n = 2) consecutively diagnosed from 2011 to 2022 by flow cytometric screening for non-myeloablative HCT in a regional treatment network in Kyushu, Japan. One patient with a novel PRF1 variant escaped screening, but all patients with FHLH2 reached diagnosis and 8 of them received HCT until 3 and 9 months of age, respectively. The earliest HCT was conducted 65 days after birth. Three pretransplant deaths occurred in newborns with liver failure at diagnosis. Ten posttransplant patients have remained disease-free, 7 of whom had no neurological involvement. Time from first etoposide infusion to HCT was shorter in patients without CNS disease or bleeding than in patients with those factors (median [range] days: 62 [50–81] vs. 122 [89–209], p = 0.016). Six of 9 unrelated patients had a PRF1 c.1090_1091delCT variant. These results suggest that the critical times to start etoposide and HCT are within 3 months after birth and during etoposide control, respectively. Newborn screening may increase the percentage of disease-free survivors without complications.

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  • 後天性眼振を示したHypomyelination of Early Myelinating Structureの2歳男児

    畑井 恵理子, チョン・ピンフィー, 安倍 時子, 梶原 健太, 藤井 史彦, 園田 有里, 栂尾 理, 酒井 康成, 才津 浩智, 井上 健, 大賀 正一

    脳と発達   56 ( Suppl. )   S191 - S191   2024.5   ISSN:0029-0831 eISSN:1884-7668

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  • 後天性眼振を示したHypomyelination of Early Myelinating Structureの2歳男児

    畑井 恵理子, チョン・ピンフィー, 安倍 時子, 梶原 健太, 藤井 史彦, 園田 有里, 栂尾 理, 酒井 康成, 才津 浩智, 井上 健, 大賀 正一

    脳と発達   56 ( Suppl. )   S191 - S191   2024.5   ISSN:0029-0831 eISSN:1884-7668

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  • ヒト誘導ミクログリアが示す多様な免疫応答はミトコンドリア脆弱性に関連する

    米元 耕輔, 藤井 史彦, 平良 遼志, 扇谷 昌宏, 奥園 清香, チョン・ピンフィー, 吉良 龍太郎, 加藤 隆弘, 酒井 康成, 大賀 正一

    脳と発達   56 ( Suppl. )   S228 - S228   2024.5   ISSN:0029-0831 eISSN:1884-7668

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  • A first-in-human clinical study of laparoscopic autologous myoblast sheet transplantation to prevent delayed perforation after duodenal endoscopic mucosal dissection

    Kanetaka, K; Maruya, Y; Higashi, M; Yamaguchi, S; Matsumoto, R; Kobayashi, S; Hashiguchi, K; Oohashi, F; Matsumura, M; Naka, T; Sakai, Y; Nakao, K; Miyagawa, S; Eguchi, S

    STEM CELL RESEARCH & THERAPY   15 ( 1 )   117   2024.4   eISSN:1757-6512

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    Background: The detection rate of superficial non-ampullary duodenal epithelial tumors (SNADETs) has recently been increasing. Large tumors may contain malignant lesions and early therapeutic intervention is recommended. Endoscopic mucosal dissection (ESD) is considered a feasible treatment modality, however, the anatomical and physiological characteristics of the duodenum create a risk of postoperative perforation after ESD. Methods: To explore whether myoblast sheet transplantation could prevent delayed perforation after ESD, a first-in-human (FIH) clinical trial of laparoscopic autologous myoblast sheet transplantation after duodenal ESD was launched. Autologous myoblast sheets fabricated from muscle tissue obtained seven weeks before ESD were transplanted laparoscopically onto the serous side of the ESD. The primary endpoints were the onset of peritonitis due to delayed perforation within three days after surgery and all adverse events during the follow-up period. Results: Three patients with SNADETs ≥ 20 mm in size underwent transplantation of a myoblast sheet onto the serous side of the duodenum after ESD. In case 1, The patient’s postoperative course was uneventful. Endoscopy and abdominal computed tomography revealed no signs of delayed perforation. Despite incomplete mucosal closure in case 2, and multiple micro perforations during ESD in case 3, cell sheet transplantation could prevent the postoperative massive perforation after ESD, and endoscopy on day 49 after transplantation revealed no stenosis. Conclusions: This clinical trial showed the safety, efficacy, and procedural operability of this novel regenerative medicine approach involving transplanting an autologous myoblast sheet laparoscopically onto the serosa after ESD in cases with a high risk of delayed perforation. This result indicates the potential application of cell sheet medicine in treating various abdominal organs and conditions with minimal invasiveness in the future. Trial registration: jRCT, jRCT2073210094. Registered November 8 2021, https://jrct.niph.go.jp/latest-detail/jRCT2073210094.

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  • Rapid and long-lasting efficacy of high-dose ambroxol therapy for neuronopathic Gaucher disease: A case report and literature review. Reviewed International journal

    Kanako Higashi, Yuri Sonoda, Noriyuki Kaku, Fumihiko Fujii, Fumiya Yamashita, Sooyoung Lee, Vlad Tocan, Go Ebihara, Wakato Matsuoka, Kenichi Tetsuhara, Motoshi Sonoda, Pin Fee Chong, Yuichi Mushimoto, Kanako Kojima-Ishii, Masataka Ishimura, Yuhki Koga, Atsuhisa Fukuta, Nana Akagi Tsuchihashi, Yoshikazu Kikuchi, Takahito Karashima, Takaaki Sawada, Taeko Hotta, Makoto Yoshimitsu, Hideyuki Terazono, Tatsuro Tajiri, Takashi Nakagawa, Yasunari Sakai, Kimitoshi Nakamura, Shouichi Ohga

    Mol Genet Genomic Med   12 ( 4 )   e2427   2024.4   ISSN:2324-9269

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    Gaucher disease (GD) is a lysosomal storage disorder caused by a deficiency in the GBA1-encoded enzyme, β-glucocerebrosidase. Enzyme replacement therapy is ineffective for neuronopathic Gaucher disease (nGD). High-dose ambroxol has been administered as an alternative treatment for a group of patients with nGD. However, little is known about the clinical indication and the long-term outcome of patients after ambroxol therapy. We herein report a case of a female patient who presented with a progressive disease of GD type 2 from 11 months of age and had the pathogenic variants of p.L483P (formerly defined as p.L444P) and p.R502H (p.R463H) in GBA1. A combined treatment of imiglucerase with ambroxol started improving the patient's motor activity in 1 week, while it kept the long-lasting effect of preventing the deteriorating phenotype for 30 months. A literature review identified 40 patients with nGD, who had received high-dose ambroxol therapy. More than 65% of these patients favorably responded to the molecular chaperone therapy, irrespective of p.L483P homozygous, heterozygous or the other genotypes. These results highlight the long-lasting effect of ambroxol-based chaperone therapy for patients with an expanding spectrum of mutations in GBA1.

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  • Patients With Subacute Sclerosing Panencephalitis in Japan: A 2022 Nationwide Survey

    Okabe, H; Hashimoto, K; Norito, S; Kume, Y; Chishiki, M; Hasegawa, S; Sakai, Y; Nomura, K; Shibata, T; Suzuki, Y; Sunagawa, T; Takao, M; Hosoya, M

    PEDIATRIC INFECTIOUS DISEASE JOURNAL   43 ( 4 )   313 - 319   2024.4   ISSN:0891-3668 eISSN:1532-0987

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    Background: In Japan, the incidence of subacute sclerosing panencephalitis (SSPE) has reduced; however, the medical conditions and factors associated with disease progression remain unclear. Methods: A nationwide survey of SSPE was conducted using a questionnaire in 2022. We conducted a descriptive analysis of the patients with SSPE in 2022 and Cox proportional hazards analyses for disease progression. We compared the patients with SSPE with those in a 2007 survey. Results: A total of 37 surviving patients with SSPE were enrolled [median age: 32 years (range: 16-52 years)]. No new cases have been identified since 2017 in the survey. Jabbour stage IV was the most common stage (66.7%). The hazard ratios (95% confidence intervals) of male sex and age at the time of measles infection (years) were 2.56 (1.13-5.76) and 0.57 (0.34-0.93), respectively. Compared with those in 2007, the proportion of patients in hospitals decreased from 13.7% to 2.7%, whereas that of patients in nursing facilities increased from 17.6% to 29.7%. The proportions of patients prescribed inosine pranobex, interferon and ribavirin at the time of the survey decreased from 96.1% to 79.4%, 74.8% to 14.3% and 25.3% to 0%, respectively. The proportions of patients with gastrostomy, tracheostomy and ventilator use increased from 5.9% to 69.7%, 23.3% to 60.0% and 10.8% to 32.4%, respectively. Conclusions: Decreased measles cases in Japan reduced new SSPE cases. However, surviving patients in 2022 had advanced disease stages and needed medical care. Male sex and early measles infection were significantly associated with disease progression.

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  • White matter diffusion estimates in obsessive-compulsive disorder across 1653 individuals: machine learning findings from the ENIGMA OCD Working Group

    Kim B.G., Kim G., Abe Y., Alonso P., Ameis S., Anticevic A., Arnold P.D., Balachander S., Banaj N., Bargalló N., Batistuzzo M.C., Benedetti F., Bertolín S., Beucke J.C., Bollettini I., Brem S., Brennan B.P., Buitelaar J.K., Calvo R., Castelo-Branco M., Cheng Y., Chhatkuli R.B., Ciullo V., Coelho A., Couto B., Dallaspezia S., Ely B.A., Ferreira S., Fontaine M., Fouche J.P., Grazioplene R., Gruner P., Hagen K., Hansen B., Hanna G.L., Hirano Y., Höxter M.Q., Hough M., Hu H., Huyser C., Ikuta T., Jahanshad N., James A., Jaspers-Fayer F., Kasprzak S., Kathmann N., Kaufmann C., Kim M., Koch K., Kvale G., Kwon J.S., Lazaro L., Lee J., Lochner C., Lu J., Manrique D.R., Martínez-Zalacaín I., Masuda Y., Matsumoto K., Maziero M.P., Menchón J.M., Minuzzi L., Moreira P.S., Morgado P., Narayanaswamy J.C., Narumoto J., Ortiz A.E., Ota J., Pariente J.C., Perriello C., Picó-Pérez M., Pittenger C., Poletti S., Real E., Reddy Y.C.J., van Rooij D., Sakai Y., Sato J.R., Segalas C., Shavitt R.G., Shen Z., Shimizu E., Shivakumar V., Soreni N., Soriano-Mas C., Sousa N., Sousa M.M., Spalletta G., Stern E.R., Stewart S.E., Szeszko P.R., Thomas R., Thomopoulos S.I., Vecchio D., Venkatasubramanian G., Vriend C., Walitza S., Wang Z., Watanabe A., Wolters L.

    Molecular Psychiatry   29 ( 4 )   1063 - 1074   2024.4   ISSN:13594184

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    White matter pathways, typically studied with diffusion tensor imaging (DTI), have been implicated in the neurobiology of obsessive-compulsive disorder (OCD). However, due to limited sample sizes and the predominance of single-site studies, the generalizability of OCD classification based on diffusion white matter estimates remains unclear. Here, we tested classification accuracy using the largest OCD DTI dataset to date, involving 1336 adult participants (690 OCD patients and 646 healthy controls) and 317 pediatric participants (175 OCD patients and 142 healthy controls) from 18 international sites within the ENIGMA OCD Working Group. We used an automatic machine learning pipeline (with feature engineering and selection, and model optimization) and examined the cross-site generalizability of the OCD classification models using leave-one-site-out cross-validation. Our models showed low-to-moderate accuracy in classifying (1) “OCD vs. healthy controls” (Adults, receiver operator characteristic-area under the curve = 57.19 ± 3.47 in the replication set; Children, 59.8 ± 7.39), (2) “unmedicated OCD vs. healthy controls” (Adults, 62.67 ± 3.84; Children, 48.51 ± 10.14), and (3) “medicated OCD vs. unmedicated OCD” (Adults, 76.72 ± 3.97; Children, 72.45 ± 8.87). There was significant site variability in model performance (cross-validated ROC AUC ranges 51.6–79.1 in adults; 35.9–63.2 in children). Machine learning interpretation showed that diffusivity measures of the corpus callosum, internal capsule, and posterior thalamic radiation contributed to the classification of OCD from HC. The classification performance appeared greater than the model trained on grey matter morphometry in the prior ENIGMA OCD study (our study includes subsamples from the morphometry study). Taken together, this study points to the meaningful multivariate patterns of white matter features relevant to the neurobiology of OCD, but with low-to-moderate classification accuracy. The OCD classification performance may be constrained by site variability and medication effects on the white matter integrity, indicating room for improvement for future research.

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  • Non-conditioned cord blood transplantation for infection control in athymic CHARGE syndrome. Reviewed International journal

    Motoshi Sonoda, Masataka Ishimura, Hirosuke Inoue, Katsuhide Eguchi, Masayuki Ochiai, Yasunari Sakai, Takehiko Doi, Kyoko Suzuki, Takeshi Inoue, Tomoyuki Mizukami, Kimitoshi Nakamura, Hidetoshi Takada, Shouichi Ohga

    Pediatr Blood Cancer   71 ( 3 )   e30809   2024.3   ISSN:1545-5009 eISSN:1545-5017

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    OBJECTIVE: CHARGE syndrome is a congenital malformation syndrome caused by heterozygous mutations in the CHD7 gene. Severe combined immunodeficiency (SCID) arises from congenital athymia called CHARGE/complete DiGeorge syndrome. While cultured thymus tissue implantation (CTTI) provides an immunological cure, hematopoietic cell transplantation (HCT) is an alternative option for immuno-reconstitution of affected infants. We aimed to clarify the clinical outcomes of patients with athymic CHARGE syndrome after HCT. METHODS: We studied the immunological reconstitution and outcomes of four patients who received non-conditioned unrelated donor cord blood transplantation (CBT) at Kyushu University Hospital from 2007 to 2022. The posttransplant outcomes were compared with the outcomes of eight reported patients. RESULTS: Four index cases received CBT 70-144 days after birth and had no higher than grade II acute graft-versus-host disease. One infant was the first newborn-screened athymic case in Japan. They achieved more than 500/μL naïve T cells with balanced repertoire 1 month post transplant, and survived more than 12 months with home care. Twelve patients including the index cases received HCT at a median 106 days after birth (range: 70-195 days). One-year overall survival rate was significantly higher in patients who underwent non-conditioned HCT than in those who received conditioned HCT (100% vs. 37.5%, p = .02). Nine patients died, and the major cause of death was cardiopulmonary failure. CONCLUSIONS: Athymic infants achieved a prompt reconstitution of non-skewing naïve T cells after non-conditioned CBT that led to home care in infancy without significant infections. Non-conditioned CBT is a useful bridging therapy for newborn-screened cases toward an immunological cure by CTTI.

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  • High-Titer Anti-ZSCAN1 Antibodies in a Toddler Clinically Diagnosed with Apparent Rapid-Onset Obesity with Hypothalamic Dysfunction, Hypoventilation, and Autonomic Dysregulation Syndrome

    Tocan, V; Nakamura-Utsunomiya, A; Sonoda, Y; Matsuoka, W; Mizuguchi, S; Muto, Y; Hijioka, T; Nogami, M; Sasaoka, D; Nagamatsu, F; Oba, U; Kawakubo, N; Hamada, H; Mushimoto, Y; Chong, PF; Kaku, N; Koga, Y; Sakai, Y; Oda, Y; Tajiri, T; Ohga, S

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   25 ( 5 )   2024.3   ISSN:1661-6596 eISSN:1422-0067

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    Severe obesity in young children prompts for a differential diagnosis that includes syndromic conditions. Rapid-Onset Obesity with Hypothalamic Dysfunction, Hypoventilation, and Autonomic Dysregulation (ROHHAD) syndrome is a potentially fatal disorder characterized by rapid-onset obesity associated with hypoventilation, neural crest tumors, and endocrine and behavioral abnormalities. The etiology of ROHHAD syndrome remains to be established, but recent research has been focusing on autoimmunity. We report on a 2-year-old girl with rapid-onset obesity during the first year of life who progressed to hypoventilation and encephalitis in less than four months since the start of accelerated weight gain. The patient had a high titer of anti-ZSCAN1 antibodies (348; reference range < 40), and the increased values did not decline after acute phase treatment. Other encephalitis-related antibodies, such as the anti-NDMA antibody, were not detected. The rapid progression from obesity onset to central hypoventilation with encephalitis warns about the severe consequences of early-onset ROHHAD syndrome. These data indicate that serial measurements of anti-ZSCAN1 antibodies might be useful for the diagnosis and estimation of disease severity. Further research is needed to determine whether it can predict the clinical course of ROHHAD syndrome and whether there is any difference in antibody production between patients with and without tumors.

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  • Tele-proctoring for minimally invasive surgery across Japan: An initial step toward a new approach to improving the disparity of surgical care and supporting surgical education

    Takemasa, I; Okuya, K; Okita, K; Akizuki, E; Miyo, M; Ishii, M; Miura, R; Ichihara, M; Takahiro, K; Oki, E; Takatsuki, M; Eguchi, S; Ichikawa, D; Kitagawa, Y; Sakai, Y; Mori, M

    ANNALS OF GASTROENTEROLOGICAL SURGERY   8 ( 2 )   356 - 364   2024.3   ISSN:2475-0328

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    Aim: The aim of this study was to verify the clinical feasibility of tele-proctoring using our ultra-low latency communication system with shared internet access. Methods: Connections between two multiple remote locations at various distances were established through the TELEPRO® tele-proctoring system. The server records the latency between the two locations for tele-proctoring using the annotations. Questionnaires were administered to the surgeons, assistants, and medical staff. Respondents rated the quickness and quality of communication in terms of latency and disturbances in the audio, video, and usefulness of the live telestrations with annotation. Results: Seven hospitals tele-proctored with Sapporo Medical University between January 2021 and September 2022. The median latency of annotation between the two locations ranged from 24.5 to 48.5 ms. No major technological problems occurred, such as streaming interruption, loss of video or audio, poor resolution. The video encoding time was 10 ms, and its decoding time was 0.8 ms. The total latency positively correlated with the distance between two locations (R = 0.55, p < 0.01). The quality of communication regarding latency, disturbance, and surgical education with intraoperative annotative instructions showed similar trends, with perfectly fine being the most common response. No significant differences in surgical quality, educational effect, or social impact were observed between the latency ≥30 and <30 ms groups for whether the size of latency affects surgical education. Conclusion: The feasibility of the tele-proctoring system is expected to be a sustainable approach to help education for young surgeons and surgical supports in rural areas, thereby reducing disparities in health care.

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  • The Etiology and Outcome of Area Postrema Syndrome in Childhood: Two Cases and a Literature Review

    Tomari, Y; Igata, Y; Chong, PF; Kajiwara, K; Hatai, E; Sonoda, Y; Oba, U; Kaku, N; Koga, Y; Sakai, Y; Ohga, S

    PEDIATRIC NEUROLOGY   152   11 - 15   2024.3   ISSN:0887-8994 eISSN:1873-5150

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    Background: Area postrema syndrome (APS), a rare childhood condition, manifests as intractable nausea and hiccups. APS has high diagnostic significance in neuromyelitis optica syndrome spectrum disorders (NMOSD) and can be the initial presentation of other critical diseases, including brainstem glioma. Methods: We described two representative cases of unrelated Japanese patients with APS. An etiologic evaluation, including a detailed intracranial neuroradiological examination and autoantibodies assessment, was performed. We also reviewed the literature focusing on the prognosis of pediatric APS symptoms. Results: A 14-year-old girl with aquaporin-4 antibody-positive NMOSD showed a good prognosis with immunotherapy, whereas another nine-year-old girl with irresectable medullary low-grade glioma had persistent symptoms for more than 10 years. All reported children aged >12 years were diagnosed with NMOSD, and patients aged <13 years showed heterogeneous etiologies. Conclusions: Distinctive time courses and neuroimaging features were key clinical findings for the diagnostic and therapeutic processes in these patients. This literature review highlights the wide spectrum and prognosis of pediatric-onset APS.

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  • Clinical characteristics of SARS-CoV-2-associated encephalopathy in children: Nationwide epidemiological study

    Kasai, M; Sakuma, H; Abe, Y; Kuki, I; Maegaki, Y; Murayama, K; Murofushi, Y; Nagase, H; Nishiyama, M; Okumura, A; Sakai, Y; Tada, H; Mizuguchi, M; Takanashi, JI

    JOURNAL OF THE NEUROLOGICAL SCIENCES   457   122867   2024.2   ISSN:0022-510X eISSN:1878-5883

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    Objective: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sometimes triggers acute encephalopathy as a serious neurological complication in children. We previously reported the clinico-radiological findings of SARS-CoV-2-associated encephalopathy. The advent of the SARS-CoV-2 omicron variant led to a marked increase in pediatric patients with coronavirus disease 2019 (COVID-19); however, epidemiological changes with acute encephalopathy according to the emergence of SARS-CoV-2 have not yet been documented. Therefore, the present study investigated epidemiological differences in SARS-CoV-2-associated encephalopathy during the BA.1/BA.2 and BA.5 predominant periods and also between SARS-CoV-2-associated and non-SARS-CoV-2-associated encephalopathy. Methods: We conducted a nationwide survey of SARS-CoV-2-associated encephalopathy in Japanese children between June and November 2022. We compared the present results during the BA.5 predominant period and previous findings during the BA.1/BA.2 predominant period. We also compared the clinico-radiological syndromes of encephalopathy between SARS-CoV-2-associated and non-SARS-CoV-2-associated encephalopathy. Results: Although many patients with SARS-CoV-2-associated encephalopathy in the BA.5 predominant period had seizures as their initial symptoms, no significant differences were observed in the clinical features. Patients with SARS-CoV-2-associated encephalopathy had worse outcomes than those with non-SARS-CoV-2-associated encephalopathy (p-value = 0.003). Among 103 patients with SARS-CoV-2-associated encephalopathy, 14 (13.6%) had severe types of acute encephalopathy, namely, encephalopathy with acute fulminant cerebral edema (AFCE) and hemorrhagic shock and encephalopathy syndrome (HSES). Also, 28 (27.2%) patients with SARS-CoV-2-associated encephalopathy had poor outcome: severe neurological sequelae or death. Ninety-five patients (92.2%) were not vaccinated against SARS-CoV-2. Conclusions: In SARS-CoV-2-associated encephalopathy, high percentages of AFCE and HSES can result in poor outcomes.

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  • Development of erythrocyte-mimetic PFOB/PDMS thermoplastic elastomer core-shell microparticles via SPG membrane emulsification

    Zhang, QM; Inagaki, NF; Yoshida, H; Kamihira, M; Sakai, Y; Ito, T

    JOURNAL OF MEMBRANE SCIENCE   689   2024.1   ISSN:0376-7388 eISSN:1873-3123

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    Erythrocyte-mimetic perfluorooctylbromide (PFOB)/polydimethylsiloxane (PDMS) thermoplastic elastomer core-shell microparticles were fabricated (for the first time) via Shirasu porous glass membrane emulsification (SPG ME) with evaporation-induced phase separation (EIPS). The resulting micro-sized core-shell particles showed a spontaneously concave shape without a subsequent deformation process such as organic solvent immersion or temperature variations. It replicated the healthy human erythrocyte morphology. In addition, their sizes and morphologies could be controlled by varying the pore size and dispersed-phase composition of the SPG membrane. The diameters of the precisely controlled particles were similar to those of human blood cells (9.6 ± 1.9 μm). The microcompression test revealed a high deformability of the particles owing to both softness of the PDMS-TPE shell and their core-shell structure. The PDMS-based materials displayed a high oxygen permeability, and PFOB displayed a high oxygen solubility. Finally, the oxygen transportation function was evaluated using oxygen tension-responsive HeLa cells (hr-HeLa) under hypoxic conditions (5% O<inf>2</inf>). This demonstrated the effectiveness of PFOB/PDMS-TPE microparticles as artificial oxygen carriers in various biomedical applications such as tissue engineering.

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  • Persistent intracranial hyper-inflammation in ruptured cerebral aneurysm after COVID-19: case report and review of the literature. International journal

    Pin Fee Chong, Kanako Higashi, Wakato Matsuoka, Koichi Arimura, Yuhei Sangatsuda, Katsuma Iwaki, Yuri Sonoda, Yuko Ichimiya, Akiko Kamori, Akiko Kawakami, Soichi Mizuguchi, Noriyuki Kaku, Yasunari Sakai, Shouichi Ohga

    BMC neurology   24 ( 1 )   17 - 17   2024.1   eISSN:1471-2377

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    BACKGROUND: The systemic manifestations of coronavirus disease 2019 (COVID-19) include hyperinflammatory reactions in various organs. Recent studies showed evidence for the frequent involvement of central nervous system in affected patients; however, little is known about clinical features of cerebrovascular diseases in childhood-onset COVID-19. CASE PRESENTATION: A 10-year-old boy recovered from SARS-CoV-2 infection without complication. On 14 days after infection, he presented with loss of consciousness. A head computed tomography detected a ruptured cerebral aneurysm at the left posterior cerebral artery accompanying subarachnoid hemorrhage (SAH). Immediate surgical intervention did not rescue the patient, resulting in the demise 7 days after admission. Serological and genetic tests excluded the diagnosis of vasculitis and connective tissue disorders. Retrospective analysis showed markedly higher levels of interleukin (IL)-1β, IL-6 and IL-8 in the cerebrospinal fluid than the serum sample concurrently obtained. A review of literature indicated that adult patients with COVID-19 have a risk for the later development of SAH during the convalescent phase of COVID-19. CONCLUSIONS: SAH is a severe complication of COVID-19 in children and adults who have asymptomatic cerebrovascular aneurysms. The markedly high levels of cytokines detected in the cerebrospinal fluid suggested that intracranial hyperinflammatory condition might be one of the possible mechanisms involved in the rupture of a preexisting cerebrovascular aneurysms.

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  • Spliceosome malfunction causes neurodevelopmental disorders with overlapping features

    Li, D; Wang, Q; Bayat, A; Battig, MR; Zhou, YJ; Bosch, DGM; van Haaften, G; Granger, L; Petersen, AK; Pérez-Jurado, LA; Aznar-Laín, G; Aneja, A; Hancarova, M; Bendova, S; Schwarz, M; Pourova, RK; Sedlacek, Z; Keena, BA; March, ME; Hou, CP; O'Connor, N; Bhoj, EJ; Harr, MH; Lemire, G; Boycott, KM; Towne, M; Li, M; Tarnopolsky, M; Brady, L; Parker, MJ; Faghfoury, H; Parsley, LK; Agolini, E; Dentici, ML; Novelli, A; Wright, M; Palmquist, R; Lai, K; Scala, M; Striano, P; Iacomino, M; Zara, F; Cooper, A; Maarup, TJ; Byler, M; Lebel, RR; Balci, TB; Louie, R; Lyons, M; Douglas, J; Nowak, C; Afenjar, A; Hoyer, J; Keren, B; Maas, SM; Motazacker, MM; Martinez-Agosto, JA; Rabani, AM; McCormick, EM; Falk, MJ; Ruggiero, SM; Helbig, I; Moller, RS; Tessarollo, L; Ardori, FT; Palko, ME; Hsieh, TC; Krawitz, PM; Ganapathi, M; Gelb, BD; Jobanputra, V; Wilson, A; Greally, J; Jacquemont, S; Jizi, K; Bruel, AL; Quelin, C; Misra, VK; Chick, E; Romano, C; Greco, D; Arena, A; Morleo, M; Nigro, V; Seyama, R; Uchiyama, Y; Matsumoto, N; Taira, R; Tashiro, K; Sakai, Y; Yigit, G; Wollnik, B; Wagner, M; Kutsche, B; Hurst, ACE; Thompson, ML; Schmidt, R; Randolph, L; Spillmann, RC; Shashi, V; Higginbotham, EJ; Cordeiro, D; Carnevale, A; Costain, G; Khan, T; Funalot, B; Mau-Them, FT; Moya, LFG; García-Miñaúr, S; Osmond, M; Chad, L; Quercia, N; Carrasco, D; Li, CM; Sanchez-Valle, A; Kelley, M; Nizon, M; Jensson, BO; Sulem, P; Stefansson, K; Gorokhova, S; Busa, T; Rio, M; Habdallah, HH; Lesieur-Sebellin, M; Amiel, J; Pingault, V; Mercier, S; Vincent, M; Philippe, C; Fatus-Fauconnier, C; Friend, K; Halligan, RK; Biswas, S; Rosser, J; Shoubridge, C; Corbett, M; Barnett, C; Gecz, J; Leppig, K; Slavotinek, A; Marcelis, C; Pfundt, R; de Vries, BBA; van Slegtenhorst, MA; Brooks, AS; Cogne, B; Rambaud, T; Tümer, Z; Zackai, EH; Akizu, N; Song, YQ; Hakonarson, H

    JOURNAL OF CLINICAL INVESTIGATION   134 ( 1 )   2024.1   ISSN:0021-9738 eISSN:1558-8238

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    Pre-mRNA splicing is a highly coordinated process. While its dysregulation has been linked to neurological deficits, our understanding of the underlying molecular and cellular mechanisms remains limited. We implicated pathogenic variants in U2AF2 and PRPF19, encoding spliceosome subunits in neurodevelopmental disorders (NDDs), by identifying 46 unrelated individuals with 23 de novo U2AF2 missense variants (including 7 recurrent variants in 30 individuals) and 6 individuals with de novo PRPF19 variants. Eight U2AF2 variants dysregulated splicing of a model substrate. Neuritogenesis was reduced in human neurons differentiated from human pluripotent stem cells carrying two U2AF2 hyper-recurrent variants. Neural loss of function (LoF) of the Drosophila orthologs U2af50 and Prp19 led to lethality, abnormal mushroom body (MB) patterning, and social deficits, which were differentially rescued by wild-type and mutant U2AF2 or PRPF19. Transcriptome profiling revealed splicing substrates or effectors (including Rbfox1, a third splicing factor), which rescued MB defects in U2af50deficient flies. Upon reanalysis of negative clinical exomes followed by data sharing, we further identified 6 patients with NDD who carried RBFOX1 missense variants which, by in vitro testing, showed LoF. Our study implicates 3 splicing factors as NDD-causative genes and establishes a genetic network with hierarchy underlying human brain development and function.

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  • 小児神経と生命倫理学 生命倫理学と小児神経学のトランスレーション

    笹月 桃子, トカン・ヴラッド, 酒井 康成, 吉良 龍太郎, 大賀 正一

    脳と発達   56 ( 1 )   5 - 8   2024.1   ISSN:0029-0831 eISSN:1884-7668

  • CD14 down-modulation as a real-time biomarker in Kawasaki disease. International journal

    Yutaro Inada, Motoshi Sonoda, Yumi Mizuno, Kenichiro Yamamura, Yoshitomo Motomura, Aoba Takuma, Kenji Murata, Kenji Furuno, Junichiro Tezuka, Yasunari Sakai, Shouichi Ohga, Junji Kishimoto, Koki Hosaka, Satomi Sakata, Toshiro Hara

    Clinical & translational immunology   13 ( 1 )   e1482   2024   ISSN:2050-0068 eISSN:2050-0068

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    OBJECTIVES: The objectives of this study were to investigate the pathophysiology of Kawasaki disease (KD) from immunological and oxidative stress perspectives, and to identify real-time biomarkers linked to innate immunity and oxidative stress in KD. METHODS: We prospectively enrolled 85 patients with KD and 135 patients with diverse conditions including immune, infectious and non-infectious diseases for this investigation. Flow cytometry was used to analyse the surface expression of CD14, CD38 and CD62L on monocytes, along with a quantitative assessment of CD14 down-modulation. Additionally, oxidative stress levels were evaluated using derivatives of reactive oxygen metabolites (d-ROMs) and antioxidant capacity measured by a free radical elective evaluator system. RESULTS: During the acute phase of KD, we observed a prominent CD14 down-modulation on monocytes, reflecting the indirect detection of circulating innate immune molecular patterns. Moreover, patients with KD showed a significantly higher CD14 down-modulation compared with infectious and non-infectious disease controls. Notably, the surface expression of CD14 on monocytes was restored concurrently with responses to intravenous immunoglobulin and infliximab treatment in KD. Furthermore, d-ROM levels in patients with KD were significantly elevated compared with patients with infectious and non-infectious diseases. Following intravenous immunoglobulin treatment, oxidative stress levels decreased in patients with KD. CONCLUSION: Monitoring CD14 down-modulation on monocytes in real-time is a valuable strategy for assessing treatment response, distinguishing KD relapse from concomitant infections and selecting second-line therapy after IVIG treatment in KD patients. The interplay between inflammation and oxidative stress likely plays a crucial role in the development of KD.

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  • Translational medicine of bioethics and child neurology : A short essay

    Sasazuki Momoko, Tocan Vlad, Sakai Yasunari, Kira Ryutaro, Ohga Shouichi

    NO TO HATTATSU   56 ( 1 )   5 - 8   2024   ISSN:00290831 eISSN:18847668

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    <p>  Recent progress in genetic analysis and therapeutics provide patients and families with a hope for the cure of intractable diseases. The current age also enforces pediatricians to think over the complex and diverse decision-making processes and therapeutic options for each patient. Presumably, this situation will become more complex within the range of legal and ethical consensus for therapeutic options that pediatricians must consider as advocates for ill children. We cannot simplify or streamline the process for how they support the interests of children and what they should protect. When standing at the critical point of decision-making for a life-threatening condition, pediatricians need to develop agreement with their patients and families through persistent discussions without setting up a goal for agreement among them. Thus, translational research in pediatrics may represent the search for essence deeply embedded in bioethics, which may possibly lead us to untangling the conundrums in clinical practice.</p>

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  • Decellularized Mouse Liver as a Small-Scale Scaffold for the Creation of a Miniaturized Human Liver

    Ijima, H; Fukuda, Y; Uehara, MK; Shafiq, M; Wu, FQ; Cho, JY; Sakai, Y; Miyata, T; Yamao, T; Nakao, Y; Kuroda, Y; Motomura, T; Yamashita, YI; Baba, H

    JOURNAL OF CHEMICAL ENGINEERING OF JAPAN   56 ( 1 )   2023.12   ISSN:0021-9592 eISSN:1881-1299

  • Progressive myoclonic epilepsy as an expanding phenotype of NGLY1-associated congenital deglycosylation disorder: A case report and review of the literature. Reviewed International journal

    Yuri Sonoda, Atsushi Fujita, Michiko Torio, Takahiko Mukaino, Ayumi Sakata, Masaru Matsukura, Kousuke Yonemoto, Ken Hatae, Yuko Ichimiya, Pin Fee Chong, Masayuki Ochiai, Yoshinao Wada, Machiko Kadoya, Nobuhiko Okamoto, Yoshiko Murakami, Tadashi Suzuki, Noriko Isobe, Hiroshi Shigeto, Naomichi Matsumoto, Yasunari Sakai, Shouichi Ohga

    Eur J Med Genet   67   104895 - 104895   2023.12   ISSN:1769-7212 eISSN:1878-0849

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    INTRODUCTION: NGLY1-associated congenital disorder of deglycosylation (CDDG1: OMIM #615273) is a rare autosomal recessive disorder caused by a functional impairment of endoplasmic reticulum in degradation of glycoproteins. Neurocognitive dysfunctions have been documented in patients with CDDG1; however, deteriorating phenotypes of affected individuals remain elusive. CASE PRESENTATION: A Japanese boy with delayed psychomotor development showed ataxic movements from age 5 years and myoclonic seizures from age 12 years. Appetite loss, motor and cognitive decline became evident at age 12 years. Electrophysiological studies identified paroxysmal discharges on myoclonic seizure and a giant somatosensory evoked potential. Perampanel was effective for controlling myoclonic seizures. Exome sequencing revealed that the patient carried compound heterozygous variants in NGLY1, NM_018297.4: c.857G > A and c.-17_12del, which were inherited from mother and father, respectively. A literature review confirmed that myoclonic seizures were observed in 28.5% of patients with epilepsy. No other patients had progressive myoclonic epilepsy or cognitive decline in association with loss-of-function variations in NGLY1. CONCLUSION: Our data provides evidence that a group of patients with CDDG1 manifest slowly progressive myoclonic epilepsy and cognitive decline during the long-term clinical course.

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  • Subcutaneous angiogenesis induced by transdermal delivery of gel-in-oil nanogel dispersion

    Zhang, Y; Fardous, J; Inoue, Y; Doi, R; Obata, A; Sakai, Y; Aishima, S; Ijima, H

    BIOMATERIALS ADVANCES   154   213628   2023.11   eISSN:2772-9508

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    Subcutaneous transplantation aims to enhance the growth and functionality of transplanted cells for therapeutic outcomes in tissue engineering. However, the limited subcutaneous vascular network poses a challenge. Conventional methods involve co-transplantation with endothelial cells or angiogenic scaffold implantation, but they have drawbacks like tissue inflammation, compromised endothelial cell functionality, and the risk of repeated scaffold transplantation. Effective techniques are needed to overcome these challenges. This study explores the potential of G/O-NGD, a gel-in-oil nanogel dispersion, as a transdermal carrier of proliferative factors to promote angiogenesis in subcutaneous graft beds before cell transplantation. We observed robust subcutaneous angiogenesis by delivering varying amounts of bFGF using the G/O-NGD emulsion. Quantitative analysis of several parameters confirmed the efficacy of this method for building a subcutaneous vascular network. G/O-NGD is a biodegradable material that facilitates localized transdermal delivery of bFGF while maintaining its activity. The findings of this study have significant implications in both medical and industrial fields.

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  • Bimodal artificial intelligence using TabNet for differentiating spinal cord tumors-Integration of patient background information and images

    Kita, K; Fujimori, T; Suzuki, Y; Kanie, Y; Takenaka, S; Kaito, T; Taki, T; Ukon, Y; Furuya, M; Saiwai, H; Nakajima, N; Sugiura, T; Ishiguro, H; Kamatani, T; Tsukazaki, H; Sakai, Y; Takami, H; Tateiwa, D; Hashimoto, K; Wataya, T; Nishigaki, D; Sato, J; Hoshiyama, M; Tomiyama, N; Okada, S; Kido, S

    ISCIENCE   26 ( 10 )   2023.10   eISSN:2589-0042

  • TRPV4‐mediated Ca2+ deregulation causes mitochondrial dysfunction via the AKT/α‐synuclein pathway in dopaminergic neurons Reviewed International journal

    Xiao Sun, Jun Kong, Shuangshan Dong, Hiroki Kato, Hiroshi Sato, Yuta Hirofuji, Yosuke Ito, Lu Wang, Takahiro A. Kato, Michiko Torio, Yasunari Sakai, Shouichi Ohga, Satoshi Fukumoto, Keiji Masuda

    FASEB BioAdv   5 ( 12 )   507 - 520   2023.10   ISSN:2573-9832 eISSN:2573-9832

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    <jats:title>Abstract</jats:title><jats:p>Mutations in the gene encoding the transient receptor potential vanilloid member 4 (TRPV4), a Ca<jats:sup>2+</jats:sup> permeable nonselective cation channel, cause TRPV4‐related disorders. TRPV4 is widely expressed in the brain; however, the pathogenesis underlying TRPV4‐mediated Ca<jats:sup>2+</jats:sup> deregulation in neurodevelopment remains unresolved and an effective therapeutic strategy remains to be established. These issues were addressed by isolating mutant dental pulp stem cells from a tooth donated by a child diagnosed with metatropic dysplasia with neurodevelopmental comorbidities caused by a gain‐of‐function TRPV4 mutation, c.1855C &gt; T (p.L619F). The mutation was repaired using CRISPR/Cas9 to generate corrected isogenic stem cells. These stem cells were differentiated into dopaminergic neurons and the pharmacological effects of folic acid were examined. In mutant neurons, constitutively elevated cytosolic Ca<jats:sup>2+</jats:sup> augmented AKT‐mediated α‐synuclein (α‐syn) induction, resulting in mitochondrial Ca<jats:sup>2+</jats:sup> accumulation and dysfunction. The TRPV4 antagonist, AKT inhibitor, or α‐syn knockdown, normalizes the mitochondrial Ca<jats:sup>2+</jats:sup> levels in mutant neurons, suggesting the importance of mutant TRPV4/Ca<jats:sup>2+</jats:sup>/AKT‐induced α‐syn in mitochondrial Ca<jats:sup>2+</jats:sup> accumulation. Folic acid was effective in normalizing mitochondrial Ca<jats:sup>2+</jats:sup> levels via the transcriptional repression of α‐syn and improving mitochondrial reactive oxygen species levels, adenosine triphosphate synthesis, and neurite outgrowth of mutant neurons. This study provides new insights into the neuropathological mechanisms underlying TRPV4‐related disorders and related therapeutic strategies.</jats:p>

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  • The functional connectome in obsessive-compulsive disorder: resting-state mega-analysis and machine learning classification for the ENIGMA-OCD consortium

    Bruin, WB; Abe, Y; Alonso, P; Anticevic, A; Backhausen, LL; Balachander, S; Bargallo, N; Batistuzzo, MC; Benedetti, F; Triquell, SB; Brem, S; Calesella, F; Couto, B; Denys, DAJP; Echevarria, MAN; Eng, GK; Ferreira, S; Feusner, JD; Grazioplene, RG; Gruner, P; Guo, JY; Hagen, K; Hansen, B; Hirano, Y; Hoexter, MQ; Jahanshad, N; Jaspers-Fayer, F; Kasprzak, S; Kim, M; Koch, K; Kwak, YB; Kwon, JS; Lazaro, L; Li, CSR; Lochner, C; Marsh, R; Martínez-Zalacaín, I; Menchon, JM; Moreira, PS; Morgado, P; Nakagawa, A; Nakao, T; Narayanaswamy, JC; Nurmi, EL; Zorrilla, JCP; Piacentini, J; Picó-Pérez, M; Piras, F; Piras, F; Pittenger, C; Reddy, JYC; Rodriguez-Manrique, D; Sakai, Y; Shimizu, E; Shivakumar, V; Simpson, BH; Soriano-Mas, C; Sousa, N; Spalletta, G; Stern, ER; Stewart, SE; Szeszko, PR; Tang, JS; Thomopoulos, S; Thorsen, AL; Tokiko, Y; Tomiyama, H; Vai, B; Veer, IM; Venkatasubramanian, G; Vetter, NC; Vriend, C; Walitza, S; Waller, L; Wang, Z; Watanabe, A; Wolff, N; Yun, JY; Zhao, Q; van Leeuwen, WA; van Marle, HJF; van de Mortel, LA; van der Straten, A; van der Werf, YD; Thompson, PM; Stein, DJ; van den Heuvel, OA; van Wingen, GA

    MOLECULAR PSYCHIATRY   28 ( 10 )   4307 - 4319   2023.10   ISSN:1359-4184 eISSN:1476-5578

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    Current knowledge about functional connectivity in obsessive-compulsive disorder (OCD) is based on small-scale studies, limiting the generalizability of results. Moreover, the majority of studies have focused only on predefined regions or functional networks rather than connectivity throughout the entire brain. Here, we investigated differences in resting-state functional connectivity between OCD patients and healthy controls (HC) using mega-analysis of data from 1024 OCD patients and 1028 HC from 28 independent samples of the ENIGMA-OCD consortium. We assessed group differences in whole-brain functional connectivity at both the regional and network level, and investigated whether functional connectivity could serve as biomarker to identify patient status at the individual level using machine learning analysis. The mega-analyses revealed widespread abnormalities in functional connectivity in OCD, with global hypo-connectivity (Cohen’s d: -0.27 to -0.13) and few hyper-connections, mainly with the thalamus (Cohen’s d: 0.19 to 0.22). Most hypo-connections were located within the sensorimotor network and no fronto-striatal abnormalities were found. Overall, classification performances were poor, with area-under-the-receiver-operating-characteristic curve (AUC) scores ranging between 0.567 and 0.673, with better classification for medicated (AUC = 0.702) than unmedicated (AUC = 0.608) patients versus healthy controls. These findings provide partial support for existing pathophysiological models of OCD and highlight the important role of the sensorimotor network in OCD. However, resting-state connectivity does not so far provide an accurate biomarker for identifying patients at the individual level.

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  • The functional connectome in obsessive-compulsive disorder: resting-state mega-analysis and machine learning classification for the ENIGMA-OCD consortium (May, 10.1038/s41380-023-02077-0, 2023)

    Bruin, WB; Abe, Y; Alonso, P; Anticevic, A; Backhausen, LL; Balachander, S; Bargallo, N; Batistuzzo, MC; Benedetti, F; Triquell, SB; Brem, S; Calesella, F; Couto, B; Denys, DAJP; Echevarria, MAN; Eng, GK; Ferreira, S; Feusner, JD; Grazioplene, RG; Gruner, P; Guo, JY; Hagen, K; Hansen, B; Hirano, Y; Hoexter, MQ; Jahanshad, N; Jaspers-Fayer, F; Kasprzak, S; Kim, M; Koch, K; Kwak, YB; Kwon, JS; Lazaro, L; Li, CSR; Lochner, C; Marsh, R; Martínez-Zalacaín, I; Menchon, JM; Moreira, PS; Morgado, P; Nakagawa, A; Nakao, T; Narayanaswamy, JC; Nurmi, EL; Zorrilla, JCP; Piacentini, J; Picó-Pérez, M; Piras, F; Piras, F; Pittenger, C; Reddy, JYC; Rodriguez-Manrique, D; Sakai, Y; Shimizu, E; Shivakumar, V; Simpson, BH; Soriano-Mas, C; Sousa, N; Spalletta, G; Stern, ER; Stewart, SE; Szeszko, PR; Tang, JS; Thomopoulos, SI; Thorsen, AL; Yoshida, T; Tomiyama, H; Vai, B; Veer, IM; Venkatasubramanian, G; Vetter, NC; Vriend, C; Walitza, S; Waller, L; Wang, Z; Watanabe, A; Wolff, N; Yun, JY; Zhao, Q; van Leeuwen, WA; van Marle, HJF; van de Mortel, LA; van der Straten, A; van der Werf, YD; Arai, H; Bollettini, I; Escalona, RC; Coelho, A; Colombo, F; Darwich, L; Fontaine, M; Ikuta, T; Ipser, JC; Juaneda-Seguí, A; Kitagawa, H; Kvale, G; Machado-Sousa, M; Morer, A; Nakamae, T; Narumoto, J; O'Neill, J; Okawa, S; Real, E; Roessner, V; Sato, JR; Segalàs, C; Shavitt, RG; Veltman, DJ; Yamada, K; Thompson, PM; Stein, DJ; van den Heuvel, OA; van Wingen, GA

    MOLECULAR PSYCHIATRY   28 ( 10 )   4320 - 4320   2023.10   ISSN:1359-4184 eISSN:1476-5578

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    Correction to: Molecular Psychiatry, published online 2 May 2023 In this article Honami Arai, Irene Bollettini, Rosa Calvo Escalona, Ana Coelho, Federica Colombo, Leila Darwich, Martine Fontaine, Toshikazu Ikuta, Jonathan C. Ipser, Asier Juaneda-Seguí, Hitomi Kitagawa, Gerd Kvale, Mafalda Machado-Sousa, Astrid Morer, Takashi Nakamae, Jin Narumoto, Joseph O’Neill, Sho Okawa, Eva Real, Veit Roessner, Joao R. Sato, Cinto Segalàs, Roseli G. Shavitt, Dick J. Veltman, Kei Yamada were missing from the author list indexed under the ENIGMA-OCD Working Group. Additionally, there was an error regarding Tokiko Yoshida’s name, where the first name and last name were written in the wrong order. The original article has been corrected.

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  • Effect of extracellular matrix fiber cross-linkage on cancer cell motility and surrounding matrix deformation

    Omata, S; Fukuda, K; Sakai, Y; Ohuchida, K; Morita, Y

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   673   44 - 50   2023.9   ISSN:0006-291X eISSN:1090-2104

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    Cancer incidence is increasing annually, and the invasion of cancer into the stroma significantly affects cancer metastasis. The stroma primarily comprises an abundant extracellular matrix (ECM) that interacts closely with cancer cells. Cancer cells use the ECM as a scaffold to migrate from a tumor via mechanical actions such as pushing and pulling the fibers. The purpose of this study is to clarify the effects of elastic modulus differences on cell migration behavior based on the same ECM fiber structure. We observe temporal changes in the morphology of cancer cells and the surrounding ECM to elucidate the relationship between changes in the mechanical properties of the ECM and the invasive behavior of cancer cells. We analyze the shape and migration distance of cancer cells and the displacement field of the ECM by varying the fiber elastic modulus but fixing the ECM density. Increasing the elastic modulus results in a protruding cell shape, which indicates the maximum displacement of the ECM around the cell. Additionally, differences in cell migration speed and dispersion based on the elastic modulus are observed. The behavior of cells with increasing elasticity is classified via cluster analysis. Owing to the chemical cross-linking of the fibers, some cells cannot deform the surrounding tissue. This is attributable to the gel state of the ECM and microscopic fluctuations in the fiber density around the cells. We successfully assessed the effect of changes in the ECM modulus on cell mortality and morphology to reveal the mechanism of cancer invasion.

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  • Angiogenic and inflammatory responses in human induced microglia-like (iMG) cells from patients with Moyamoya disease. Reviewed International journal

    Noritoshi Shirozu, Masahiro Ohgidani, Nobuhiro Hata, Shunya Tanaka, Shogo Inamine, Noriaki Sagata, Tetsuaki Kimura, Ituro Inoue, Koichi Arimura, Akira Nakamizo, Ataru Nishimura, Naoki Maehara, Soh Takagishi, Katsuma Iwaki, Tomohiro Nakao, Keiji Masuda, Yasunari Sakai, Masahiro Mizoguchi, Koji Yoshimoto, Takahiro A Kato

    Sci Rep   13 ( 1 )   14842 - 14842   2023.9   ISSN:2045-2322

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    Angiogenic factors associated with Moyamoya disease (MMD) are overexpressed in M2 polarized microglia in ischemic stroke, suggesting that microglia may be involved in the pathophysiology of MMD; however, existing approaches are not applicable to explore this hypothesis. Herein we applied blood induced microglial-like (iMG) cells. We recruited 25 adult patients with MMD and 24 healthy volunteers. Patients with MMD were subdivided into progressive (N = 7) or stable (N = 18) group whether novel symptoms or radiographic advancement of Suzuki stage within 1 year was observed or not. We produced 3 types of iMG cells; resting, M1-, and M2-induced cells from monocytes, then RNA sequencing followed by GO and KEGG pathway enrichment analysis and qPCR assay were performed. RNA sequencing of M2-induced iMG cells revealed that 600 genes were significantly upregulated (338) or downregulated (262) in patients with MMD. Inflammation and immune-related factors and angiogenesis-related factors were specifically associated with MMD in GO analysis. qPCR for MMP9, VEGFA, and TGFB1 expression validated these findings. This study is the first to demonstrate that M2 microglia may be involved in the angiogenic process of MMD. The iMG technique provides a promising approach to explore the bioactivity of microglia in cerebrovascular diseases.

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  • Effects of melatonin on dopaminergic neuron development via IP3-mediated mitochondrial Ca2+ regulation in autism spectrum disorder. Reviewed International journal

    Dong S, Kifune T, Kato H, Wang L, Kong J, Hirofuji Y, Xiao Sun, Sato H, Ito Y, Kato TA, Sakai Y, Ohga S, Fukumoto S, Masuda K

    Biochem Biophys Res Commun   681   7 - 12   2023.9   ISSN:0006-291X eISSN:1090-2104

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    Melatonin entrainment of suprachiasmatic nucleus-regulating circadian rhythms is mediated by MT1 and MT2 receptors. Melatonin also has neuroprotective and mitochondrial activating effects, suggesting it may affect neurodevelopment. We studied melatonin's pharmacological effects on autism spectrum disorder (ASD) neuropathology. Deciduous tooth-derived stem cells from children with ASD were used to model neurodevelopmental defects and differentiated into dopaminergic neurons (ASD-DNs) with or without melatonin. Without melatonin, ASD-DNs had reduced neurite outgrowth, mitochondrial dysfunction, lower mitochondrial Ca<sup>2+</sup> levels, and Ca<sup>2+</sup> accumulation in the endoplasmic reticulum (ER) compared to control DNs from typically developing children-derived stem cells. Melatonin enhanced IP3-dependent Ca<sup>2+</sup> release from ER to mitochondria, improving mitochondrial function and neurite outgrowth in ASD-DNs. Luzindole, an MT1/MT2 antagonist, blocked these effects. Thus, melatonin supplementation may improve dopaminergic system development in ASD by modulating mitochondrial Ca<sup>2+</sup> homeostasis via MT1/MT2 receptors.

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  • Critical diseases in neonates after discharge home from birth hospital: A retrospective study from a tertiary hospital in Japan. Reviewed International journal

    Junko Fujiyoshi, Hirosuke Inoue, Toru Sawano, Yuichi Mushimoto, Yoshitomo Motomura, Kei Nishiyama, Noriyuki Kaku, Hazumu Nagata, Kenichiro Yamamura, Masataka Ishimura, Yuhki Koga, Masayuki Ochiai, Yasunari Sakai, Tatsuro Tajiri, Shouichi Ohga

    Early human development   186   105869 - 105869   2023.9   ISSN:0378-3782 eISSN:1872-6232

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    INTRODUCTION: To establish actionable neonatal screening during the first month of life, we investigated critical diseases in seemingly healthy newborns discharged from birth hospitals. METHODS: This retrospective study enrolled previously healthy full-term infants who visited our hospital, a tertiary hospital in Japan, from home between 5 and 28 days after birth from 2009 to 2018. Infants with known perinatal or congenital diseases, positive newborn screening results, or accidental injuries were excluded. Data were collected from electronic medical records, including principal diagnosis, clinical details, and prognosis at 18 months of age. RESULTS: Ninety-seven (58 %) of 168 eligible neonates were admitted to the hospital, and 71 (42 %) were not. The median admission rate in patients with disease onset at ≤14 days after birth (80 %) was significantly higher than that in patients with disease onset at ≥15 days (42 %). Among 45 patients who received intensive medical care, 5 died and 10 developed neurodevelopmental sequelae. Four of 5 patients died by 100 days. Among 25 diseases treated in intensive care unit, 17 (68 %) diseases had a prevalence of <1 per 2000 live births. The commonly used diagnostic methods were imaging (n = 58, 35 %) and physical examination (n = 34, 20 %). CONCLUSION: Critical diseases due to rare and heterogeneous causes in ostensibly healthy newborns occurred predominantly in the first two weeks of life. Optimal newborn screening and health check-up protocols may benefit from the wide spectrum of life-threatening diseases occurring in home after birth.

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  • PCDH12関連疾患にみられる発達性てんかん性脳症の臨床的特徴

    一宮 優子, 酒井 康成, 緒方 怜奈, 吉浦 孝一郎, 園田 有里, チョン・ピンフィー, 小幡 聡, 松尾 美央子, 星出 まどか, 鳥巣 浩幸, 大賀 正一

    てんかん研究   41 ( 2 )   444 - 444   2023.9   ISSN:0912-0890 eISSN:1347-5509

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  • 10年間における我が国の重症筋無力症臨床像の変化 全国疫学調査2006と同2018の比較

    吉川 弘明, 中村 好一, 栗山 長門, 村井 弘之, 酒井 康成, 野村 芳子, 松井 真, 足立 由美, 岩佐 和夫, 古川 裕, 桑原 聡

    臨床神経学   63 ( Suppl. )   S196 - S196   2023.9   ISSN:0009-918X eISSN:1882-0654

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  • PCDH12関連疾患にみられる発達性てんかん性脳症の臨床的特徴

    一宮 優子, 酒井 康成, 緒方 怜奈, 吉浦 孝一郎, 園田 有里, チョン・ピンフィー, 小幡 聡, 松尾 美央子, 星出 まどか, 鳥巣 浩幸, 大賀 正一

    てんかん研究   41 ( 2 )   444 - 444   2023.9   ISSN:0912-0890 eISSN:1347-5509

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  • Magnetization controlled by crystallization in soft magnetic Fe-Si-B-P-Cu alloys

    Nakajima, H; Osako, A; Yodoshi, N; Yamada, Y; Tsukasaki, H; Harada, K; Sakai, Y; Shigematsu, K; Nishikubo, T; Azuma, M; Mori, S

    MICROSCOPY   72 ( 4 )   274 - 278   2023.8   ISSN:2050-5698 eISSN:2050-5701

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    Soft magnetic materials have low coercive fields and high permeability. Recently, nanocrystalline alloys obtained using annealing amorphous alloys have attracted much interest since nanocrystalline alloys with small grain sizes of tens of nanometers exhibit low coercive fields comparable to that of amorphous alloys. Since nanocrystalline soft magnetic materials attain remarkable soft magnetic properties by controlling the grain size, the crystal grains' microstructure has a substantial influence on the soft magnetic properties. In this research, we examined the magnetic properties of Fe-Si-B-P-Cu nanocrystalline soft magnetic alloys obtained by annealing amorphous alloys. During crystallization, the observation findings reveal the correlation between the generated microstructures and soft magnetic properties.

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  • Effects of scan parameters on the accuracies of iodine quantification and hounsfield unit values in dual layer dual-energy head and neck computed tomography: A phantom study conducted in a hospital in Japan

    Sakai Y., Shirasaka T., Hioki K., Yamane S., Kinoshita E., Kato T.

    Radiography   29 ( 5 )   838 - 844   2023.8   ISSN:10788174

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    Introduction: No study has investigated scan parameters in head and neck dual layer dual-energy computed tomography (DL-DECT). This study aimed to select the appropriate scan parameters in head and neck imaging by evaluating the scan parameter effects on the accuracies of CT numbers and conduct iodine quantification in DL-DECT. Methods: A multi-energy phantom was scanned using a dual layer CT (DLCT) scanner. Reference materials of iodine, blood, calcium, and adipose were used. A helical scan was performed by using reference and several protocols. Iodine density and virtual monochromatic images (VMIs) at the energy of 50, 70, and 100 keV were reconstructed. The iodine concentrations and CT numbers in each protocol were measured. Moreover, the absolute percentage errors (APEs) of iodine quantifications and CT numbers (reference vs. each protocol) were compared. Equivalence was observed when APEs between reference and each protocol was within 5%. Statistical analysis was performed using appropriate software. Results: The APEs between the high-tube-voltage and reference protocol were 23.7, 14.0, 8.8, and 8.1% for iodine reference materials with concentrations equal to 2, 5, 10, and 15 mg/ml, respectively. At 50 keV, APEs between the high-tube-voltage and reference protocols were greater than 5% except for calcium and adipose. At 100 keV, APEs between the high-tube-voltage and reference protocols were greater than 5% except for blood and calcium. Conclusions: The high-tube-voltage protocol improved the accuracies of the measurement for iodine quantification and CT numbers. Additionally, the scanning parameters except for tube voltage had no effect on accuracies of iodine quantitation and CT numbers in the DLCT scanner. Implications for practice: The use of the high-tube-voltage protocol will be recommended for more accurate material decomposition in head and neck DL-DECT.

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  • Shank3a/bアイソフォームはマウスの生後早期のてんかん発作感受性および視床皮質の発達を調節する(Shank3a/b isoforms regulate the susceptibility to seizures and thalamocortical development in the early postnatal period of mice)

    Okuzono Sayaka, Fujii Fumihiko, Matsushita Yuki, Setoyama Daiki, Shinmyo Yohei, Taira Ryoji, Yonemoto Kousuke, Akamine Satoshi, Motomura Yoshitomo, Sanefuji Masafumi, Sakurai Takeshi, Kawasaki Hiroshi, Han Kihoon, Kato Takahiro A., Torisu Hiroyuki, Kang Dongchon, Nakabeppu Yusaku, Sakai Yasunari, Ohga Shouichi

    Neuroscience Research   193   13 - 19   2023.8   ISSN:0168-0102

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    マウスの生後早期の視床機能の発達におけるShank3a/bアイソフォームの役割について検討した。WTマウスとShank3a/bノックアウトを使用した。評価項目は脳の各領域における定量PCR、カイニン酸治療、免疫蛍光染色、ウェスタンブロット法などとした。その結果、マウスShank3スプライシングアイソフォームShank3a/bは視床核で特異的に発現し、生後2~4週間でピークに達することが示された。また、Shank3a/bノックアウトマウスでは視床核のパルブアルブミンが低かった。一貫して、Shank3a/bノックアウトマウスは、カイニン酸処理後、WTマウスと比較して全般発作を起こしやすいことが示された。以上から、Shank3a/bのNT-Ankドメインは、マウス生後早期において、視床皮質ニューロンを過剰興奮から守る分子経路を制御していることが示された。

  • COVID-19罹患後に脳動脈瘤破裂によるくも膜下出血を合併した男児

    家守 章子, チョン・ピンフィー, 一宮 優子, 園田 有里, 酒井 康成, 大賀 正一, 有村 公一, 東 加奈子, 松岡 若利, 川上 晶子, 水口 壮一, 賀来 典之

    日本小児科学会雑誌   127 ( 7 )   1017 - 1017   2023.7   ISSN:0001-6543

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  • 健常児として自宅退院した新生児に発症した重篤な疾患の検討

    藤吉 順子, 井上 普介, 澤野 徹, 虫本 雄一, 本村 良知, 賀来 典之, 永田 弾, 山村 健一郎, 石村 匡崇, 古賀 友紀, 落合 正行, 酒井 康成, 大賀 正一, 永田 公二, 田尻 達郎

    日本小児科学会雑誌   127 ( 7 )   1015 - 1015   2023.7   ISSN:0001-6543

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  • Regulation of reactive oxygen species and phytohormones in osmotic stress tolerance during seed germination in <i>indica</i> rice

    Kawaguchi, R; Suriyasak, C; Matsumoto, R; Sawada, Y; Sakai, Y; Hamaoka, N; Sasaki, K; Yamane, K; Kato, Y; Bailly, C; Ishibashi, Y

    FRONTIERS IN PLANT SCIENCE   14   1186960   2023.6   ISSN:1664-462X

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    Climate change due to global warming is now affecting agricultural production worldwide. In rice, one of the most important crops, water limitation due to irregular rainfall in rainfed lowlands during crop growth limits yield. Dry direct-sowing has been proposed as a water-efficient approach to cope with water stress during rice growth, but poor seedling establishment due to drought during germination and emergence is a problem. Here, we germinated indica rice cultivars Rc348 (drought tolerant) and Rc10 (drought sensitive) under osmotic stress induced by PEG to elucidate mechanisms of germination under drought. Rc348 had higher germination rate and germination index under severe osmotic stress of −1.5 MPa, above those of Rc10. Rc348 showed up-regulated GA biosynthesis, down-regulated ABA catabolism, and up-regulated α-amylase gene expression in imbibed seeds under PEG treatment compared to that of Rc10. During germination, reactive oxygen species (ROS) play important roles in antagonism between gibberellic acid (GA) and abscisic acid (ABA). Embryo of Rc348 treated with PEG had significantly greater expression of NADPH oxidase genes and higher endogenous ROS levels, together with significantly increased endogenous GA<inf>1</inf>, GA<inf>4</inf> and ABA contents compared to that of Rc10. In aleurone layers treated with exogenous GA, expression of α-amylase genes was higher in Rc348 than in Rc10, and expression of NADPH oxidase genes was enhanced with significantly higher ROS content in Rc348, suggesting higher sensitivity of GA to ROS production and starch degradation in aleurone cells of Rc348. These results suggest that the osmotic stress tolerance of Rc348 is due to enhancement of ROS production, GA biosynthesis, and GA sensitivity, resulting in a higher germination rate under osmotic stress.

    DOI: 10.3389/fpls.2023.1186960

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  • [Possible Radiation Dose Reduction in Abdominal Plain CT Using Deep Learning Reconstruction].

    Onizuka Y, Sakai Y, Shirasaka T, Kondo M, Kato T

    Nihon Hoshasen Gijutsu Gakkai zasshi   79 ( 5 )   446 - 452   2023.5   ISSN:0369-4305

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    DOI: 10.6009/jjrt.2023-1289

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  • COVID-19にともなう軽症急性脳症の8歳男児

    家守 章子, 園田 有里, 一宮 優子, チョン・ピンフィー, 水口 壮一, 賀来 典之, 金政 光, 本村 良知, 酒井 康成, 大賀 正一

    脳と発達   55 ( Suppl. )   S398 - S398   2023.5   ISSN:0029-0831 eISSN:1884-7668

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  • COVID-19にともなう軽症急性脳症の8歳男児

    家守 章子, 園田 有里, 一宮 優子, チョン・ピンフィー, 水口 壮一, 賀来 典之, 金政 光, 本村 良知, 酒井 康成, 大賀 正一

    脳と発達   55 ( Suppl. )   S398 - S398   2023.5   ISSN:0029-0831 eISSN:1884-7668

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  • 極低出生体重児が6歳までに示す神経発達の変動 単一施設コホート

    緒方 怜奈, 安永 由紀恵, 渡辺 恭子, チョン・ピンフィー, 岡本 潤, 酒見 好弘, 中嶋 敏紀, 大野 拓郎, 園田 有里, 一宮 優子, 井上 普介, 落合 正行, 酒井 康成, 山下 博徳, 大賀 正一

    脳と発達   55 ( Suppl. )   S410 - S410   2023.5   ISSN:0029-0831 eISSN:1884-7668

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  • 超早産児の修正1歳半から修正3歳のDQスコア上昇には身長の伸びが関連する

    松永 友佳, 井上 普介, 落合 正行, チョン・ピンフィー, 酒井 康成, 大賀 正一

    脳と発達   55 ( Suppl. )   S329 - S329   2023.5   ISSN:0029-0831 eISSN:1884-7668

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  • ヒト誘導ミクログリアを用いた炎症性シグナルの解析

    米元 耕輔, 藤井 史彦, 平良 遼志, 扇谷 昌宏, 奥園 清香, チョン・ピンフィー, 吉良 龍太郎, 加藤 隆弘, 酒井 康成, 大賀 正一

    脳と発達   55 ( Suppl. )   S297 - S297   2023.5   ISSN:0029-0831 eISSN:1884-7668

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  • 先天性心疾患のフォローアップ中に硬膜動静脈瘻と診断された21 trisomyの6歳女児

    園田 有里, 石倉 稔也, 一宮 優子, チョン・ピンフィー, 金政 光, 本村 良知, 平田 悠一郎, 有村 公一, 中溝 玲, 酒井 康成, 大賀 正一

    脳と発達   55 ( Suppl. )   S388 - S388   2023.5   ISSN:0029-0831 eISSN:1884-7668

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  • 先天性心疾患のフォローアップ中に硬膜動静脈瘻と診断された21 trisomyの6歳女児

    園田 有里, 石倉 稔也, 一宮 優子, チョン・ピンフィー, 金政 光, 本村 良知, 平田 悠一郎, 有村 公一, 中溝 玲, 酒井 康成, 大賀 正一

    脳と発達   55 ( Suppl. )   S388 - S388   2023.5   ISSN:0029-0831 eISSN:1884-7668

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  • Stroke in a protein C-deficient infant after stem cell transplant for CHARGE syndrome. Reviewed International journal

    Atsushi Tanaka, Yoshiaki Sakaguchi, Hirosuke Inoue, Naoki Egami, Yuri Sonoda, Motoshi Sonoda, Masataka Ishimura, Masayuki Ochiai, Taeko Hotta, Takeshi Uchiumi, Yasunari Sakai, Shouichi Ohga

    Pediatr Blood Cancer   70 ( 4 )   e30047   2023.4   ISSN:1545-5009 eISSN:1545-5017

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    DOI: 10.1002/pbc.30047

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  • 頭蓋内電極留置を経て焦点切除手術を行なった小児てんかん症例の治療成績

    下川 能史, 森岡 隆人, 村上 信哉, 橋口 公章, 迎 伸孝, 重藤 寛史, 酒井 康成, 酒田 あゆみ, 渡邉 恵利子, 吉本 幸司

    小児の脳神経   48 ( 2 )   196 - 196   2023.4   ISSN:0387-8023 eISSN:2435-824X

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  • Molecular diagnosis of 405 individuals with autism spectrum disorder. Reviewed International journal

    Noriko Miyake, Yoshinori Tsurusaki, Ryoko Fukai, Itaru Kushima, Nobuhiko Okamoto, Kei Ohashi, Kazuhiko Nakamura, Ryota Hashimoto, Yoko Hiraki, Shuraku Son, Mitsuhiro Kato, Yasunari Sakai, Hitoshi Osaka, Kimiko Deguchi, Toyojiro Matsuishi, Saoko Takeshita, Aviva Fattal-Valevski, Nina Ekhilevitch, Jun Tohyama, Patrick Yap, Wee Teik Keng, Hiroshi Kobayashi, Keiyo Takubo, Takashi Okada, Shinji Saitoh, Yuka Yasuda, Toshiya Murai, Kazuyuki Nakamura, Shouichi Ohga, Ayumi Matsumoto, Ken Inoue, Tomoko Saikusa, Tova Hershkovitz, Yu Kobayashi, Mako Morikawa, Aiko Ito, Toshiro Hara, Yota Uno, Chizuru Seiwa, Kanako Ishizuka, Emi Shirahata, Atsushi Fujita, Eriko Koshimizu, Satoko Miyatake, Atsushi Takata, Takeshi Mizuguchi, Norio Ozaki, Naomichi Matsumoto

    Eur J Hum Genet   32 ( 12 )   1551 - 1558   2023.3   ISSN:1018-4813 eISSN:1476-5438

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    Autism spectrum disorder (ASD) is caused by combined genetic and environmental factors. Genetic heritability in ASD is estimated as 60-90%, and genetic investigations have revealed many monogenic factors. We analyzed 405 patients with ASD using family-based exome sequencing to detect disease-causing single-nucleotide variants (SNVs), small insertions and deletions (indels), and copy number variations (CNVs) for molecular diagnoses. All candidate variants were validated by Sanger sequencing or quantitative polymerase chain reaction and were evaluated using the American College of Medical Genetics and Genomics/Association for Molecular Pathology guidelines for molecular diagnosis. We identified 55 disease-causing SNVs/indels in 53 affected individuals and 13 disease-causing CNVs in 13 affected individuals, achieving a molecular diagnosis in 66 of 405 affected individuals (16.3%). Among the 55 disease-causing SNVs/indels, 51 occurred de novo, 2 were compound heterozygous (in one patient), and 2 were X-linked hemizygous variants inherited from unaffected mothers. The molecular diagnosis rate in females was significantly higher than that in males. We analyzed affected sibling cases of 24 quads and 2 quintets, but only one pair of siblings shared an identical pathogenic variant. Notably, there was a higher molecular diagnostic rate in simplex cases than in multiplex families. Our simulation indicated that the diagnostic yield is increasing by 0.63% (range 0-2.5%) per year. Based on our simple simulation, diagnostic yield is improving over time. Thus, periodical reevaluation of ES data should be strongly encouraged in undiagnosed ASD patients.

    DOI: 10.1038/s41431-023-01335-7

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  • 心疾患患者における5回立ち上がり検査から運動耐容能は推定可能か?

    石田 昂彬, 山本 周平, 三澤 加代子, 常田 亮介, 酒井 康成, 矢嶋 史恵, 樋口 智子, 池上 章太, 堀内 博志

    理学療法研究・長野   ( 51 )   1 - 6   2023.3   ISSN:1347-2976

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    外来心臓リハビリテーションで心肺運動負荷試験(CPX)と身体機能評価を行った心疾患患者40例(平均年齢61.2±11.4歳)を対象に、5回立ち上がり検査(5STS)と運動耐容能の関連性を明確にし、運動耐容能評価としての5STSの有用性について検討した。CPXから得られた最高酸素摂取量(Peak VO2)とPeak METs、身体機能評価として膝伸展筋力、握力、片脚立位時間、5STS、10m歩行の最大および快適速度を調査し、Peak VO2と各指標との相関を算出した。その結果、Peak VO2と有意な相関を認めた身体機能評価項目は年齢、5STS、10m最大歩行速度と片脚立位時間であった。運動耐容能を推定する上で最も優れた身体機能評価項目は5STSであり、5METs以上の運動耐容能を有する5STSのカットオフ値は7.28秒(感度80.9%、特異度78.9%)であった。

  • 先天性横隔膜ヘルニア(congenital diaphragmatic hernia;CDH)児の3歳時発達予後

    澤野 徹, 井上 普介, 落合 正行, 酒井 康成, 大賀 正一, 藤田 恭之, 加藤 聖子, 近藤 琢也, 永田 公二, 田尻 達郎

    日本小児科学会雑誌   127 ( 3 )   470 - 470   2023.3   ISSN:0001-6543

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  • Severe pediatric acute encephalopathy syndromes related to SARS-CoV-2

    Sakuma, H; Takanashi, JI; Muramatsu, K; Kondo, H; Shiihara, T; Suzuki, M; Okanari, K; Kasai, M; Mitani, O; Nakazawa, T; Omata, T; Shimoda, K; Abe, Y; Maegaki, Y; Murayama, K; Murofushi, Y; Nagase, H; Okumura, A; Sakai, Y; Tada, H; Mizuguchi, M

    FRONTIERS IN NEUROSCIENCE   17   1085082   2023.2   ISSN:16624548 eISSN:1662-453X

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    Background and objectives: To clarify whether severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection cause acute encephalopathy in children and which are the most common syndromes that cause them and what are the outcomes. Methods: A nationwide web-based survey among all members of the Japanese Society of Child Neurology to identify pediatric patients aged < 18 years who developed acute encephalopathy in Japan between 1 January 2020 and 31 May 2022 associated with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection confirmed by polymerase chain reaction or antigen tests using pharyngeal swabs. Acute encephalopathy was defined as acute onset of impaired consciousness lasting > 24 h or an altered mental state; neurological symptoms arising within 2 weeks of onset of COVID-19 or multisystem inflammatory syndrome in children (MIS-C)/pediatric inflammatory multisystem syndrome (PIMS); evidence of SARS-CoV-2 infection; and reasonable exclusion of other diseases. Patients were divided into the known clinico-radiological acute encephalopathy syndrome group and unexplained or unclassifiable acute encephalopathy group. Outcomes were assessed by pediatric cerebral performance category (PCPC) score at hospital discharge. Results: Of the 3,802 society members, 217 representing institutions responded, and 39 patients with suspected acute encephalopathy were reported, of which 31 met inclusion criteria. Of these patients, 14 were diagnosed with known clinico-radiological acute encephalopathy syndromes, with acute encephalopathy with biphasic seizures and late reduced diffusion (five patients) being the most common. Five developed acute encephalopathy associated with MIS-C/PIMS. Among 31 patients, 9 (29.0%) had severe sequelae or died (PCPC ≥ 4). Two of three patients with encephalopathy with acute fulminant cerebral edema and two with hemorrhagic shock and encephalopathy syndrome died. The PCPC scores were higher in the known clinico-radiological acute encephalopathy syndrome group than in the unexplained or unclassifiable acute encephalopathy group (P < 0.01). Discussion: Acute encephalopathy related to SARS-CoV-2 infection was demonstrated to be more severe than that caused by other viruses in Japan. Acute encephalopathy syndromes characterized by specific neuroradiological findings was associated with poor clinical outcomes.

    DOI: 10.3389/fnins.2023.1085082

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  • Input optics systems of the KAGRA detector during O3GK

    Akutsu, T; Ando, M; Arai, K; Arai, Y; Araki, S; Araya, A; Aritomi, N; Asada, H; Aso, Y; Bae, S; Bae, Y; Baiotti, L; Bajpai, R; Barton, MA; Cannon, K; Cao, Z; Capocasa, E; Chan, M; Chen, C; Chen, K; Chen, Y; Chiang, C; Chu, H; Chu, YK; Eguchi, S; Enomoto, Y; Flaminio, R; Fujii, Y; Fujikawa, Y; Fukunaga, M; Fukushima, M; Furuhata, T; Gao, D; Ge, GG; Ha, S; Hagiwara, A; Haino, S; Han, WB; Hasegawa, K; Hattori, K; Hayakawa, H; Hayama, K; Himemoto, Y; Hiranuma, Y; Hirata, N; Hirose, E; Hong, Z; Hsieh, BH; Huang, GZ; Huang, HY; Huang, P; Huang, YC; Huang, YJ; Hui, DCY; Ide, S; Ikenoue, B; Imam, S; Inayoshi, K; Inoue, Y; Ioka, K; Ito, K; Itoh, Y; Izumi, K; Jeon, C; Jin, HB; Jung, K; Jung, P; Kaihotsu, K; Kajita, T; Kakizaki, M; Kamiizumi, M; Kanbara, S; Kanda, N; Kang, G; Kataoka, Y; Kawaguchi, K; Kawai, N; Kawasaki, T; Kim, C; Kim, J; Kim, JC; Kim, WS; Kim, YM; Kimura, N; Kita, N; Kitazawa, H; Kojima, Y; Kokeyama, K; Komori, K; Kong, AKH; Kotake, K; Kozakai, C; Kozu, R; Kumar, R; Kume, J; Kuo, C; Kuo, HS; Kuromiya, Y; Kuroyanagi, S; Kusayanagi, K; Kwak, K; Lee, HK; Lee, HW; Lee, R; Leonardi, M; Li, KL; Lin, LCC; Lin, CY; Lin, FK; Lin, FL; Lin, HL; Liu, GC; Luo, LW; Majorana, E; Marchio, M; Michimura, Y; Mio, N; Miyakawa, O; Miyamoto, A; Miyazaki, Y; Miyo, K; Miyoki, S; Mori, Y; Morisaki, S; Moriwaki, Y; Nagano, K; Nagano, S; Nakamura, K; Nakano, H; Nakano, M; Nakashima, R; Nakayama, Y; Narikawa, T; Naticchioni, L; Negishi, R; Quynh, LN; Ni, WT; Nishizawa, A; Nozaki, S; Obuchi, Y; Ogaki, W; Oh, JJ; Oh, K; Oh, SH; Ohashi, M; Ohishi, N; Ohkawa, M; Ohta, H; Okutani, Y; Okutomi, K; Oohara, K; Ooi, C; Oshino, S; Otabe, S; Pan, KC; Pang, H; Parisi, A; Park, J; Arellano, FEP; Pinto, ; Sago, N; Saito, S; Saito, Y; Sakai, K; Sakai, Y; Sakuno, Y; Sato, S; Sato, T; Sawada, T; Sekiguchi, T; Sekiguchi, Y; Shao, L; Shibagaki, S; Shimizu, R; Shimoda, T; Shimode, K; Shinkai, H; Shishido, T; Shoda, A; Somiya, K; Son, EJ; Sotani, H; Sugimoto, R; Suresh, J; Suzuki, T; Suzuki, T; Tagoshi, H; Takahashi, H; Takahashi, R; Takamori, A; Takano, S; Takeda, H; Takeda, M; Tanaka, H; Tanaka, K; Tanaka, K; Tanaka, T; Tanaka, T; Tanioka, S; San Martín, ENT; Telada, S; Tomaru, T; Tomigami, Y; Tomura, T; Travasso, F; Trozzo, L; Tsang, T; Tsao, JS; Tsubono, K; Tsuchida, S; Tsutsui, T; Tsuzuki, T; Tuyenbayev, D; Uchikata, N; Uchiyama, T; Ueda, A; Uehara, T; Ueno, K; Ueshima, G; Uraguchi, F; Ushiba, T; Putten, MHPM; Vocca, H; Wang, J; Washimi, T; Wu, C; Wu, H; Wu, S; Xu, WR; Yamada, T; Yamamoto, K; Yamamoto, K; Yamamoto, T; Yamashita, K; Yamazaki, R; Yang, Y; Yano, K; Yokogawa, K; Yokoyama, J; Yokozawa, T; Yoshioka, T; Yuzurihara, H; Zeidler, S; Zhan, M; Zhang, H; Zhao, Y; Zhu, ZH; Goetz, R; Heintze, M; Liu, J; Müller, C; Savage, RL; Tanner, DB

    PROGRESS OF THEORETICAL AND EXPERIMENTAL PHYSICS   2023 ( 2 )   2023.2   ISSN:2050-3911

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    KAGRA, the underground and cryogenic gravitational-wave detector, was operated for its solo observation from February 25 to March 10, 2020, and its first joint observation with the GEO 600 detector from April 7 to April 21, 2020 (O3GK). This study presents an overview of the input optics systems of the KAGRA detector, which consist of various optical systems, such as a laser source, its intensity and frequency stabilization systems, modulators, a Faraday isolator, mode-matching telescopes, and a high-power beam dump. These optics were successfully delivered to the KAGRA interferometer and operated stably during the observations. The laser frequency noise was observed to limit the detector sensitivity above a few kilohertz, whereas the laser intensity did not significantly limit the detector sensitivity.

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  • 新生児健診のこれから-2021 大学病院へ緊急受診を要した新生児の基礎疾患

    落合 正行, 藤吉 順子, 井上 普介, 澤野 徹, 虫本 雄一, 本村 良和, 賀来 典之, 永田 弾, 山村 健一郎, 石村 匡崇, 古賀 友紀, 酒井 康成, 大賀 正一

    日本小児科学会雑誌   127 ( 2 )   170 - 170   2023.2   ISSN:0001-6543

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  • Shank3aは生後早期マウスのけいれん感受性を制御する

    奥園 清香, 藤井 史彦, 松下 悠紀, 瀬戸山 大樹, 新明 洋平, 平良 遼志, 米元 耕輔, 赤峰 哲, 本村 良知, 實藤 雅文, 櫻井 武, 河崎 洋志, Han Kihoon, 加藤 隆弘, 鳥巣 浩幸, 康 東天, 中別府 雄作, 酒井 康成, 大賀 正一

    日本小児科学会雑誌   127 ( 2 )   193 - 193   2023.2   ISSN:0001-6543

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  • Paradoxical spells during ACTH treatment in an infant with Tetralogy of Fallot. International journal

    Rie Kikuno, Kenichiro Yamamura, Yusaku Nagatomo, Hazumu Nagata, Yuko Ichimiya, Yasunari Sakai, Shouichi Ohga

    Pediatrics international : official journal of the Japan Pediatric Society   65 ( 1 )   e15503   2023.1   ISSN:1328-8067 eISSN:1442-200X

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    DOI: 10.1111/ped.15503

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  • 静脈洞血栓,頭蓋内石灰化から硬膜動静脈瘻の診断に至った21 trisomyの6歳女児

    石倉 稔也, 園田 有里, 一宮 優子, チョン・ピンフィー, 本村 良知, 平田 悠一郎, 有村 公一, 中溝 玲, 酒井 康成, 大賀 正一

    脳と発達   55 ( 1 )   66 - 66   2023.1   ISSN:0029-0831 eISSN:1884-7668

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  • Fallot四徴症でACTH治療中に奇異性の発作がみられた乳児例(Paradoxical spells during ACTH treatment in an infant with Tetralogy of Fallot)

    Kikuno Rie, Yamamura Kenichiro, Nagatomo Yusaku, Nagata Hazumu, Ichimiya Yuko, Sakai Yasunari, Ohga Shouichi

    Pediatrics International   65 ( 1 )   ped.15503 - ped.15503   2023   ISSN:1328-8067

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    症例は9ヵ月齢男児。Down症候群とFallot四徴症に罹患していた。右室流出路閉塞は比較的軽症であり、出生以降、チアノーゼまたは無酸素発作のエピソードはみられていなかった。9ヵ月齢になってから点頭発作を伴う強直性攣縮がみられ始めた。脳波検査でヒプスアリスミアが示されたことからWest症候群と確定診断され、副腎皮質刺激ホルモン(ACTH)治療を行うため当院へ入院となった。入院6日目にACTH治療を開始したが、同治療6日目の哺乳後に徐脈(心拍数60bpm)とチアノーゼを発症した。心エコー法では右室流出路閉塞の悪化と肺血流量の減少が示された。無酸素発作を疑い、体位をchest-knee positionとしたところ、容量輸液を行わずとも約30分後に発作は回復した。以降も徐脈がみられ無酸素発作を間欠的に4回発症したことからACTH治療は中止した。その中止から2日後の心拍数は110bpmまで回復し、無酸素発作はもはやみられなくなっていた。ACTH中止後はトピラマートを使用した。以降も無酸素発作または攣縮発作はみられず退院した。14ヵ月齢時に心内修復術が施行され、術後経過に問題はみられず、6ヵ月間の経過観察中にもてんかん性攣縮は再発しなかった。

  • Associations of medication with subcortical morphology across the lifespan in OCD: Results from the international ENIGMA Consortium

    Ivanov, I; Boedhoe, PSW; Abe, Y; Alonso, P; Ameis, SH; Arnold, PD; Balachander, S; Baker, JT; Banaj, N; Bargalló, N; Batistuzzo, MC; Benedetti, F; Beucke, JC; Bollettini, I; Brem, S; Brennan, BP; Buitelaar, J; Calvo, R; Cheng, YQ; Cho, KIK; Dallaspezia, S; Denys, D; Diniz, JB; Ely, BA; Feusner, JD; Ferreira, S; Fitzgerald, KD; Fontaine, M; Gruner, P; Hanna, GL; Hirano, Y; Hoexter, MQ; Huyser, C; Ikari, K; James, A; Jaspers-Fayer, F; Jiang, HY; Kathmann, N; Kaufmann, C; Kim, M; Koch, K; Kwon, JS; Lázaro, L; Liu, YN; Lochner, C; Marsh, R; Martínez-Zalacaín, I; Mataix-Cols, D; Menchón, JM; Minuzzi, L; Morer, A; Morgado, P; Nakagawa, A; Nakamae, T; Nakao, T; Narayanaswamy, JC; Nurmi, EL; Oh, S; Perriello, C; Piacentini, JC; Picó-Pérez, M; Piras, F; Piras, F; Reddy, YCJ; Manrique, DR; Sakai, Y; Shimizu, E; Simpson, HB; Soreni, N; Soriano-Mas, C; Spalletta, G; Stern, ER; Stevens, MC; Stewart, SE; Szeszko, PR; Tolin, DF; van Rooij, D; Veltman, DJ; van der Werf, YD; van Wingen, GA; Venkatasubramanian, G; Walitza, S; Wang, Z; Watanabe, A; Wolters, LH; Xu, XF; Yun, JY; Zarei, M; Zhang, FR; Zhao, Q; Jahanshad, N; Thomopoulos, SI; Thompson, PM; Stein, DJ; van den Heuvel, OA; O'Neill, J

    JOURNAL OF AFFECTIVE DISORDERS   318   204 - 216   2022.12   ISSN:0165-0327 eISSN:1573-2517

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    Background: Widely used psychotropic medications for obsessive-compulsive disorder (OCD) may change the volumes of subcortical brain structures, and differently in children vs. adults. We measured subcortical volumes cross-sectionally in patients finely stratified for age taking various common classes of OCD drugs. Methods: The ENIGMA-OCD consortium sample (1081 medicated/1159 unmedicated OCD patients and 2057 healthy controls aged 6–65) was divided into six successive 6–10-year age-groups. Individual structural MRIs were parcellated automatically using FreeSurfer into 8 regions-of-interest (ROIs). ROI volumes were compared between unmedicated and medicated patients and controls, and between patients taking serotonin reuptake inhibitors (SRIs), tricyclics (TCs), antipsychotics (APs), or benzodiazepines (BZs) and unmedicated patients. Results: Compared to unmedicated patients, volumes of accumbens, caudate, and/or putamen were lower in children aged 6–13 and adults aged 50–65 with OCD taking SRIs (Cohen's d = −0.24 to −0.74). Volumes of putamen, pallidum (d = 0.18–0.40), and ventricles (d = 0.31–0.66) were greater in patients aged 20–29 receiving APs. Hippocampal volumes were smaller in patients aged 20 and older taking TCs and/or BZs (d = −0.27 to −1.31). Conclusions: Results suggest that TCs and BZs could potentially aggravate hippocampal atrophy of normal aging in older adults with OCD, whereas SRIs may reduce striatal volumes in young children and older adults. Similar to patients with psychotic disorders, OCD patients aged 20–29 may experience subcortical nuclear and ventricular hypertrophy in relation to APs. Although cross-sectional, present results suggest that commonly prescribed agents exert macroscopic effects on subcortical nuclei of unknown relation to therapeutic response.

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  • Quantitative indices for an intracranial aneurysm and subarachnoid hemorrhage in early childhood: a case report. Reviewed International journal

    Kenichi Tetsuhara, Noriyuki Kaku, Koichi Arimura, Yasunari Sakai, Shouichi Ohga

    BMC Neurol   22 ( 1 )   488 - 488   2022.12   eISSN:1471-2377

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    BACKGROUND: Intracranial aneurysms (ICA) rarely occur in children under 3 years of age. Little is known for neuroimaging parameters that predict survival and clinical outcomes of patients with ICA in early childhood. CASE PRESENTATION: A 2-year-old girl showed intracranial hemorrhage due to a rupture of aneurysm at the middle cerebral artery. Quantitative measurements of ischemic damages on the head computed tomography (CT) marked an extremely low score of 2 points with modified Alberta Stroke Program Early CT Score (mASPECTS). She died 15 days after admission. In publications from 2021 to 2022, we found 21 children who were under 3 years of age at onset of ICA. None of them died, but two of three patients who had mASPECTS scores 0-8 showed developmental delay and/or epilepsy as neurological complications. CONCLUSION: Early CT findings are applicable for predicting survival and neurological outcomes of young children with intracranial hemorrhage.

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  • MLAGO: machine learning-aided global optimization for Michaelis constant estimation of kinetic modeling

    Maeda K., Hatae A., Sakai Y., Boogerd F.C., Kurata H.

    BMC Bioinformatics   23 ( 1 )   2022.12

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    Background: Kinetic modeling is a powerful tool for understanding the dynamic behavior of biochemical systems. For kinetic modeling, determination of a number of kinetic parameters, such as the Michaelis constant (K<inf>m</inf>), is necessary, and global optimization algorithms have long been used for parameter estimation. However, the conventional global optimization approach has three problems: (i) It is computationally demanding. (ii) It often yields unrealistic parameter values because it simply seeks a better model fitting to experimentally observed behaviors. (iii) It has difficulty in identifying a unique solution because multiple parameter sets can allow a kinetic model to fit experimental data equally well (the non-identifiability problem). Results: To solve these problems, we propose the Machine Learning-Aided Global Optimization (MLAGO) method for K<inf>m</inf> estimation of kinetic modeling. First, we use a machine learning-based K<inf>m</inf> predictor based only on three factors: EC number, KEGG Compound ID, and Organism ID, then conduct a constrained global optimization-based parameter estimation by using the machine learning-predicted K<inf>m</inf> values as the reference values. The machine learning model achieved relatively good prediction scores: RMSE = 0.795 and R<sup>2</sup> = 0.536, making the subsequent global optimization easy and practical. The MLAGO approach reduced the error between simulation and experimental data while keeping K<inf>m</inf> values close to the machine learning-predicted values. As a result, the MLAGO approach successfully estimated K<inf>m</inf> values with less computational cost than the conventional method. Moreover, the MLAGO approach uniquely estimated K<inf>m</inf> values, which were close to the measured values. Conclusions: MLAGO overcomes the major problems in parameter estimation, accelerates kinetic modeling, and thus ultimately leads to better understanding of complex cellular systems. The web application for our machine learning-based K<inf>m</inf> predictor is accessible at https://sites.google.com/view/kazuhiro-maeda/software-tools-web-apps, which helps modelers perform MLAGO on their own parameter estimation tasks.

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  • Genetic and clinical landscape of childhood cerebellar hypoplasia and atrophy

    Sakamoto, M; Iwama, K; Sasaki, M; Ishiyama, A; Komaki, H; Saito, T; Takeshita, E; Shimizu-Motohashi, Y; Haginoya, K; Kobayashi, T; Goto, T; Tsuyusaki, Y; Iai, M; Kurosawa, K; Osaka, H; Tohyama, J; Kobayashi, Y; Okamoto, N; Suzuki, Y; Kumada, S; Inoue, K; Mashimo, H; Arisaka, A; Kuki, I; Saijo, H; Yokochi, K; Kato, M; Inaba, Y; Gomi, Y; Saitoh, S; Shirai, K; Morimoto, M; Izumi, Y; Watanabe, Y; Nagamitsu, SI; Sakai, Y; Fukumura, S; Muramatsu, K; Ogata, T; Yamada, K; Ishigaki, K; Hirasawa, K; Shimoda, K; Akasaka, M; Kohashi, K; Sakakibara, T; Ikuno, M; Sugino, N; Yonekawa, T; Gürsoy, S; Cinleti, T; Kim, CA; Teik, KW; Yan, CM; Haniffa, M; Ohba, C; Ito, S; Saitsu, H; Saida, K; Tsuchida, N; Uchiyama, Y; Koshimizu, E; Fujita, A; Hamanaka, K; Misawa, K; Miyatake, S; Mizuguchi, T; Miyake, N; Matsumoto, N

    GENETICS IN MEDICINE   24 ( 12 )   2453 - 2463   2022.12   ISSN:1098-3600 eISSN:1530-0366

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    Purpose: Cerebellar hypoplasia and atrophy (CBHA) in children is an extremely heterogeneous group of disorders, but few comprehensive genetic studies have been reported. Comprehensive genetic analysis of CBHA patients may help differentiating atrophy and hypoplasia and potentially improve their prognostic aspects. Methods: Patients with CBHA in 176 families were genetically examined using exome sequencing. Patients with disease-causing variants were clinically evaluated. Results: Disease-causing variants were identified in 96 of the 176 families (54.5%). After excluding 6 families, 48 patients from 42 families were categorized as having syndromic associations with CBHA, whereas the remaining 51 patients from 48 families had isolated CBHA. In 51 patients, 26 aberrant genes were identified, of which, 20 (76.9%) caused disease in 1 family each. The most prevalent genes were CACNA1A, ITPR1, and KIF1A. Of the 26 aberrant genes, 21 and 1 were functionally annotated to atrophy and hypoplasia, respectively. CBHA+S was more clinically severe than CBHA–S. Notably, ARG1 and FOLR1 variants were identified in 2 families, leading to medical treatments. Conclusion: A wide genetic and clinical diversity of CBHA was revealed through exome sequencing in this cohort, which highlights the importance of comprehensive genetic analyses. Furthermore, molecular-based treatment was available for 2 families.

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  • Heat stress during grain filling regulates seed germination through alterations of DNA methylation in barley (<i>Hordeum vulgare</i> L.)

    Sakai, Y; Suriyasak, C; Inoue, M; Hamaoka, N; Ishibashi, Y

    PLANT MOLECULAR BIOLOGY   110 ( 4-5 )   325 - 332   2022.11   ISSN:0167-4412 eISSN:1573-5028

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    Key message: Alterations in DNA methylation levels of ROS, GA and ABA related gene promoters cause transcriptional changes upon imbibition to induce seed germination in barley seeds exposed to heat stress during grain filling. Abstract: Environmental changes, especially changes in temperature, during seed development affect germination in several plant species. We have previously shown that heat stress during rice grain filling alters DNA methylation, an epigenetic mark important for gene silencing, regulates transcript levels of phytohormone metabolism genes, and delays seed germination. However, whether this phenomenon is present in other plant species remained to be elucidated. In this study, we compared seeds germination of barley (Hordeum vulgare L.) plants grown at 15 °C (control) or 25 °C (heat stress) during grain filling. Heat stress during grain filling significantly promoted seed germination in comparison with the control. The phytohormone gibberellic acid (GA) and reactive oxygen species produced by NADPH oxidases promote seed germination, whereas phytohormone abscisic acid (ABA) suppresses seed germination. We found that in heat-stressed seeds, genes related to ABA biosynthesis (HvNCED1 and 2) were significantly suppressed, whereas genes related to ABA catabolism (HvABA8’OH) and GA biosynthesis (HvHA20ox, HvGA3ox), and NADPH oxidase (HvRboh) genes were significantly upregulated after imbibition. Using MeDIP-qPCR, we showed that the promoters of HvNCED were hyper-methylated, and those of HvABA8’OH1, HvABA8’OH3, HvGA3ox2, and HvRbohF2 were hypo-methylated in heat treated seeds. Taken together, our data suggest that heat stress during grain filling affects DNA methylation of germination-related genes and promotes seed germination in barley.

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  • TOPICS 小児科学 川崎病の病態と酸化リン脂質

    中島 康貴, 酒井 康成, 原 寿郎

    医学のあゆみ   283 ( 3 )   217 - 218   2022.10   ISSN:00392359

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    DOI: 10.32118/ayu28303217

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  • Vascular patches comprising polycaprolactone/decellularized extracellular matrix (PCL/dECM)-based core/shell nanofibers for arterial healing and vascular reconstruction

    Shafiq, M; Koyanagi, K; Shirakigawa, N; Sakai, Y; Ijima, H

    TISSUE ENGINEERING PART A   28   20 - 20   2022.10   ISSN:1937-3341 eISSN:1937-335X

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  • MS2 第5回全国調査からみる多発性硬化症の二次性進行型に移行するリスク因子の検討

    渡邉 充, 磯部 紀子, 新野 正明, 中島 一郎, 松下 拓也, 酒井 康成, 中原 仁, 河内 泉, 越智 博文, 中辻 裕司, 中村 好一, 中村 幸志, 坂田 清美, 松井 真, 桑原 聡, 吉良 潤一

    神経免疫学   27 ( 1 )   159 - 159   2022.10   ISSN:0918-936X

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  • MS2 第5回全国調査からみる多発性硬化症の二次性進行型に移行するリスク因子の検討

    渡邉 充, 磯部 紀子, 新野 正明, 中島 一郎, 松下 拓也, 酒井 康成, 中原 仁, 河内 泉, 越智 博文, 中辻 裕司, 中村 好一, 中村 幸志, 坂田 清美, 松井 真, 桑原 聡, 吉良 潤一

    神経免疫学   27 ( 1 )   159 - 159   2022.10   ISSN:0918-936X

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  • Low-radiation dose scan protocol for preoperative imaging for dental implant surgery using deep learning-based reconstruction in multidetector CT

    Sakai, Y; Kitamoto, E; Okamura, K; Takarabe, S; Shirasaka, T; Mikayama, R; Kondo, M; Tatsumi, M; Kojima, T; Kato, T; Yoshiura, K

    ORAL RADIOLOGY   38 ( 4 )   517 - 526   2022.10   ISSN:0911-6028 eISSN:1613-9674

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    Objectives: This study aimed to investigate the impact of a deep learning-based reconstruction (DLR) technique on image quality and reduction of radiation exposure, and to propose a low-dose multidetector-row computed tomography (MDCT) scan protocol for preoperative imaging for dental implant surgery. Methods: The PB-1 phantom and a Catphan phantom 600 were scanned using volumetric scanning with a 320-row MDCT scanner. All scans were performed with a tube voltage of 120 kV, and the tube current varied from 120 to 60 to 40 to 30 mA. Images of the mandible were reconstructed using DLR. Additionally, images acquired with the 120-mA protocol were reconstructed using filtered back projection as a reference. Two observers independently graded the image quality of the mandible images using a 4-point scale (4, superior to reference; 1, unacceptable). The system performance function (SPF) was calculated to comprehensively evaluate image quality. The Wilcoxon signed-rank test was employed for statistical analysis, with statistical significance set at p value < 0.05. Results: There was no significant difference between the image quality acquired with the 40-mA tube current and reconstructed with the DLR technique (40DLR), and that acquired with the reference protocol (3.00, 3.00, p = 1.00). The SPF at 1.0 cycles/mm acquired with 40DLR was improved by 156.7% compared to that acquired with the reference protocol. Conclusions: Our proposed protocol, which achieves a two-thirds reduction in radiation dose, can provide a minimally invasive MDCT scan of acceptable image quality for dental implant surgery.

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  • Simulation of the crosstalk between glucose and acetaminophen metabolism in a liver zonation model

    Maeda K., Hagimori S., Sugimoto M., Sakai Y., Nishikawa M.

    Frontiers in Pharmacology   13   2022.9

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    The liver metabolizes a variety of substances that sometimes interact and regulate each other. The modeling of a single cell or a single metabolic pathway does not represent the complexity of the organ, including metabolic zonation (heterogeneity of functions) along with liver sinusoids. Here, we integrated multiple metabolic pathways into a single numerical liver zonation model, including drug and glucose metabolism. The model simulated the time-course of metabolite concentrations by the combination of dynamic simulation and metabolic flux analysis and successfully reproduced metabolic zonation and localized hepatotoxicity induced by acetaminophen (APAP). Drug metabolism was affected by nutritional status as the glucuronidation reaction rate changed. Moreover, sensitivity analysis suggested that the reported metabolic characteristics of obese adults and healthy infants in glucose metabolism could be associated with the metabolic features of those in drug metabolism. High activities of phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphate phosphatase in obese adults led to increased APAP oxidation by cytochrome P450 2E1. In contrast, the high activity of glycogen synthase and low activities of PEPCK and glycogen phosphorylase in healthy infants led to low glucuronidation and high sulfation rates of APAP. In summary, this model showed the effects of glucose metabolism on drug metabolism by integrating multiple pathways into a single liver metabolic zonation model.

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  • Two-step nationwide epidemiological survey of myasthenia gravis in Japan 2018

    Yoshikawa, H; Adachi, Y; Nakamura, Y; Kuriyama, N; Murai, H; Nomura, Y; Sakai, Y; Iwasa, K; Furukawa, Y; Kuwabara, S; Matsui, M

    PLOS ONE   17 ( 9 )   e0274161   2022.9   ISSN:1932-6203

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    Objective To study the updated prevalence and clinical features of myasthenia gravis (MG) in Japan during 2017. Methods We sent survey sheets to the randomly selected medical departments (number = 7,545). First, we asked the number of MG patients who visited medical departments from January 1, 2017, to December 31, 2017. Then, we sent the second survey sheet to the medical departments that answered the first survey to obtain the clinical information of patients who received MG diagnosis between January 1, 2015, and December 31, 2017. Results The received answer to the first survey were 2,708 (recovery rate: 35.9%). After all, the prevalence of the 100,000 population was estimated as 23.1 (95%CI: 20.5-25.6). As a result of the second survey, we obtained 1,464 case records. After checking the duplications and lacking data, we utilized 1,195 data for further analysis. The median [interquartile range (IQR)] from the onset age of total patients was 59 (43-70) years old. The male-female ratio was 1: 1.15. The onset age [median (IQR)] for female patients was 58 (40-72) years old, and that for male patients was 60 (49-69) years old (Wilcoxon-Mann-Whitney test, p = 0.0299). We divided patients into four categories: 1) anti-acetylcholine receptor antibody (AChRAb) (+) thymoma (Tm) (-), 2) AChRAb(+)Tm(+), 3) anti-muscle-specific kinase antibody (MuSKAb) (+), and AChRAb(-)MuSKAb(-) (double negative; DN). The onset age [median (IQR)] of AChRAb(+)Tm(-) was 64 (48-73) years old, and AChRb(+)Tm(+) was 55 (45-66), MuSKAb(+) was 49 (36-64), DN was 47 (35-60) year old. The multivariate logistic regression analysis using sex, initial symptoms, repetitive nerve stimulation test (RNST), and edrophonium test revealed that sex, ocular symptoms, bulbar symptoms, and RNST were factors to distinguish each category. The myasthenia gravis activities of daily living profile at the severest state were significantly higher in MuSKAb(+). MuSKAb(+) frequently received prednisolone, tacrolimus plasmapheresis, and intravenous immunoglobulin; however, they received less acetylcholine esterase inhibitor. 99.2% of AChRAb(+)Tm(+) and 15.4% of AChRAb(+)Tm(-) received thymectomy. MuSKAb(+) did not receive thymectomy, and only 5.7% of DN received thymectomy. The prognosis was favorable in all categories. Conclusion Our result revealed that the prevalence of Japanese MG doubled from the previous study using the same survey method in 2006. We also found that the onset age shifted to the elderly, and the male-female ratio reached almost even. Classification in four categories; AChRAb(+)Tm(-), AChRAb(+)Tm(+), MuSKAb(+), and DN, well describe the specific clinical features of each category and differences in therapeutic approaches.

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  • Nationwide survey of Lambert-Eaton myasthenic syndrome in Japan

    Yoshikawa, H; Adachi, Y; Nakamura, Y; Kuriyama, N; Murai, H; Nomura, Y; Sakai, Y; Iwasa, K; Furukawa, Y; Kuwabara, S; Matsui, M

    BMJ NEUROLOGY OPEN   4 ( 2 )   e000291   2022.9   eISSN:2632-6140

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    Background: There was no nationwide epidemiological study of Lambert-Eaton myasthenic syndrome (LEMS) in Japan; therefore, we conducted a nationwide survey. Methods: For the first survey, we sent survey sheets to randomly selected medical departments (n=7545) to obtain the number of LEMS who visited medical departments between 1 January 2017 and 31 December 2017. For the second survey, we sent survey sheets to the corresponding medical departments to obtain clinical information on LEMS. Results: We received 2708 responses (recovery rate: 35.9%) to the first survey. We estimated the number of LEMS as 348 (95% CI 247 to 449). The prevalence was 2.7 (95% CI 1.9 to 3.5) in 1 000 000 population. As a result of the second survey, we obtained 30 case records of 16 men and 14 women. Fourteen patients (46.7%) had a tumour, and 10 out of 14 tumours were small-cell lung carcinoma (71.4%). There was a predominance of men in the LEMS with tumour (paraneoplastic LEMS, P-LEMS) (n=11, 78.6%) and women in the LEMS without tumour (a primary autoimmune form of LEMS, AI-LEMS) (n=11, 68.8%) (p=0.0136). The onset age (mean (SD)) for the P-LEMS was 67.1 (9.0), and that for AI-LEMS was 57.8 (11.2) years old (p=0.0103). The disease duration (median) for P-LEMS was 2 years, and for AI-LEMS was 7.5 years (p=0.0134). Conclusions: The prevalence of LEMS in Japan is similar to that in other countries. There are predominances of men in P-LEMS and women in AI-LEMS.

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  • A Faster Reduction of the Dynamic Time Warping Distance to the Longest Increasing Subsequence Length

    Sakai, Y; Inenaga, S

    ALGORITHMICA   84 ( 9 )   2581 - 2596   2022.9   ISSN:0178-4617 eISSN:1432-0541

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    The similarity between a pair of time series, i.e., sequences of indexed values in time order, is often estimated by the dynamic time warping (DTW) distance, instead of any in the well-studied family of measures including the longest common subsequence (LCS) length and the edit distance. Although it may seem as if the DTW and the LCS(-like) measures are essentially different, we reveal that the DTW distance can be represented by the longest increasing subsequence (LIS) length of a sequence of integers, which is the LCS length between the integer sequence and itself sorted. For a given pair of time series of length n such that the dissimilarity between any elements is an integer between zero and c, we propose an integer sequence that represents any substring-substring DTW distance as its band-substring LIS length. The length of the produced integer sequence is O(cn<sup>2</sup>) , which can be translated to O(n<sup>2</sup>) for constant dissimilarity functions. To demonstrate that techniques developed under the LCS(-like) measures are directly applicable to analysis of time series via our reduction of DTW to LIS, we present time-efficient algorithms for DTW-related problems utilizing the semi-local sequence comparison technique developed for LCS-related problems.

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  • Importance of the heart rate in ultra-high-resolution coronary CT angiography with 0.35 s gantry rotation time

    Kojima, T; Shirasaka, T; Yamasaki, Y; Kondo, M; Hamasaki, H; Mikayama, R; Sakai, Y; Kato, T; Nishie, A; Ishigami, K; Yabuuchi, H

    JAPANESE JOURNAL OF RADIOLOGY   40 ( 8 )   781 - 790   2022.8   ISSN:1867-1071 eISSN:1867-108X

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    Purpose: We investigated the effects of the heart rate (HR) on the motion artifact in coronary computed tomography angiography (CCTA) with ultra-high-resolution-CT (U-HRCT), and we clarified the upper limit of optimal HR in CCTA with U-HRCT in a comparison with conventional-resolution-CT (CRCT) on a cardiac phantom and in patients with CCTA. Materials and methods: A pulsating cardiac phantom equipped with coronary models was scanned at static and HR simulations of 40–90 beats/min (bpm) at 10-bpm intervals using U-HRCT and CRCT, respectively. The sharpness and lumen diameter of the coronary model were quantitatively compared between U-HRCT and CRCT stratified by HR in the phantom study. We also assessed the visual inspections of clinical images in CCTA with U-HRCT. Results: At the HRs ≤ 60 bpm, the error of the lumen diameter of the U-HRCT tended to be smaller than that of the CRCT. However, at the HRs > 60 bpm, the inverse was shown. For the image sharpness, the U-HRCT was significantly superior to the CRCT (p < 0.05). In the visual assessment, the scores were negatively correlated with HRs in patients (Spearman r = − 0.71, p < 0.01). A receiver-operating characteristic analysis revealed the HR of 61 bpm as the optimal cutoff of the non-diagnostic image quality, with an area under the curve of 0.87, 95% sensitivity, and 71% specificity. Conclusion: At HRs ≤ 60 bpm, U-HRCT was more accurate in the imaging of coronary arteries than CRCT. The upper limit of the optimal HR in CCTA with U-HRCT was approx. 60 bpm.

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  • Translational pediatrics: clinical perspective for Phelan-McDermid syndrome and autism research

    Sakai, Y; Okuzono, S; Schaaf, CP; Ohga, S

    PEDIATRIC RESEARCH   92 ( 2 )   373 - 377   2022.8   ISSN:0031-3998 eISSN:1530-0447

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    Abstract: Phelan–McDermid syndrome (PMS) is a rare genetic disorder presenting with developmental delay, epilepsy, and autism spectrum disorder (ASD). The segmental deletion of chromosome 22q13.3 affects the copy number of SHANK3, the gene encoding a scaffolding protein at the postsynaptic density. Biological studies indicate that SHANK3 plays crucial roles in the development of synaptic functions in the postnatal brain. Notably, induced pluripotent stem (iPS) cells have enabled researchers to develop brain organoids and microglia from patients and to explore the pathophysiology of neurodevelopmental disorders in human cells. Single-cell RNA sequencing of these cells revealed that human-specific genes are uniquely expressed during cortical development. Thus, patient-derived disease models are expected to identify as-yet-unidentified functions of SHANK3 in the development of human brain. These efforts may help establish a new style of translational research in pediatrics, which is expected to provide therapeutic insight for children with PMS and broader categories of disease. Impact: Phelan–McDermid syndrome is a prototypic model for molecular studies of autism spectrum disorder.Brain organoids are expected to provide therapeutic insight.Single-cell RNA sequencing of microglia may uncover the functional roles of human-specific genes.

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  • てんかん外科術後にde novo精神病を呈した3症例

    三苫 良, 下川 能史, 迎 伸孝, 酒井 康成, 重藤 寛史, 酒田 あゆみ, 渡邊 恵利子, 平野 昭吾, 平野 羊嗣

    てんかん研究   40 ( 2 )   464 - 464   2022.8   ISSN:0912-0890 eISSN:1347-5509

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  • Mitochondrial Calcium-Triggered Oxidative Stress and Developmental Defects in Dopaminergic Neurons Differentiated from Deciduous Teeth-Derived Dental Pulp Stem Cells with MFF Insufficiency. Reviewed International journal

    Xiao Sun, Shuangshan Dong, Hiroki Kato, Jun Kong, Yosuke Ito, Yuta Hirofuji, Hiroshi Sato, Takahiro A Kato, Yasunari Sakai, Shouichi Ohga, Satoshi Fukumoto, Keiji Masuda

    Antioxidants   11 ( 7 )   2022.7   ISSN:2076-3921 eISSN:2076-3921

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    Mitochondrial fission factor (MFF) is an adapter that targets dynamin-related protein 1 from the cytosol to the mitochondria for fission. Loss-of-function MFF mutations cause encephalopathy due to defective mitochondrial and peroxisomal fission 2 (EMPF2). To elucidate the molecular mechanisms that were involved, we analyzed the functional effects of MFF depletion in deciduous teeth-derived dental pulp stem cells differentiating into dopaminergic neurons (DNs). When treated with MFF-targeting small interfering RNA, DNs showed impaired neurite outgrowth and reduced mitochondrial signals in neurites harboring elongated mitochondria. MFF silencing also caused mitochondrial Ca2+ accumulation through accelerated Ca2+ influx from the endoplasmic reticulum (ER) via the inositol 1,4,5-trisphosphate receptor. Mitochondrial Ca2+ overload led DNs to produce excessive reactive oxygen species (ROS), and downregulated peroxisome proliferator-activated receptor-gamma co-activator-1 alpha (PGC-1α). MFF was co-immunoprecipitated with voltage-dependent anion channel 1, an essential component of the ER-mitochondrial Ca2+ transport system. Folic acid supplementation normalized ROS levels, PGC-1α mediated mitochondrial biogenesis, and neurite outgrowth in MFF depleted DNs, without affecting their mitochondrial morphology or Ca2+ levels. We propose that MFF negatively regulates the mitochondrial Ca2+ influx from the ER. MFF-insufficiency recapitulated the EMPF2 neuropathology with increased oxidative stress and suppressed mitochondrial biogenesis. ROS and mitochondrial biogenesis might be potential therapeutic targets for EMPF2.

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  • Lung to thorax transverse area ratio as a predictor of neurodevelopmental outcomes in fetuses with congenital diaphragmatic hernia. Reviewed International journal

    Toru Sawano, Takuya Kondo, Go Ebihara, Kouji Nagata, Hirosuke Inoue, Junko Fujiyoshi, Masayuki Ochiai, Saki Kido, Yasuyuki Fujita, Yasunari Sakai, Kiyoko Kato, Tatsuro Tajiri, Shouichi Ohga

    Early Hum Dev   170   105598 - 105598   2022.7   ISSN:0378-3782 eISSN:1872-6232

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    INTRODUCTION: Infants with congenital diaphragmatic hernia (CDH) are at risk of neurodevelopmental disabilities. This study aimed to investigate the association between lung to thorax transverse area ratio (LTR) and neurodevelopmental outcomes at 3 years of age in fetuses with CDH. METHODS: We performed a retrospective study of infants with prenatally diagnosed isolated left-sided CDH born in Kyushu University Hospital between 2008 and 2016. We examined the association between prenatal ultrasound findings including LTR and development quotient (DQ) at 36 to 42 months of chronological age. RESULTS: We identified 34 live-born fetuses with isolated left-sided CDH, of which 30 survived and four died before discharge. The median LTR in the survivors was higher than in the non-survivors (p < 0.01). Among the survivors, 26 had available data on LTR (median 0.12, range 0.08-0.18) and overall DQ at 3 years of age (93, 61-112). Their median gestational age and birth weight were 37.6 (range 34.4-39.1) weeks and 2716 (2.256-3494) grams, respectively. There was no significant difference in overall DQ scores between the two groups divided according to the median LTR values (p = 0.62). LTR values were not associated with overall DQ scores after adjusting for gestational age (p = 0.39). In addition, no association was observed between LTR values and any subscale DQ scores. CONCLUSION: In fetuses with isolated left-sided CDH, prenatal LTR predicts the mortality but not neurodevelopmental outcomes at 3 years of age.

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  • Genotype-by-environment interaction and genetic dissection of heartwood color in Cryptomeria japonica based on multiple common gardens and quantitative trait loci mapping

    Mori H., Ueno S., Ujino-Ihara T., Fujiwara T., Yamashita K., Kanetani S., Endo R., Matsumoto A., Uchiyama K., Yoshida T., Sakai Y., Moriguchi Y., Kusano R., Tsumura Y.

    Plos One   17 ( 7 July )   2022.7

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    The heartwood color of a major plantation tree Cryptomeria japonica shows high variability among clones and cultivars, and brighter heartwood has higher value in the usage of non-laminated wood such as in traditional construction, which makes heartwood color an important trait in breeding of this species. However, the genetic basis of the interactions between genetics and the environment on heartwood color has been understudied while these are necessary for effective breeding programs in multiple environmental condition. The objectives of the present study were to evaluate the effects of genetics and environments on heartwood color and how they interact in contrasting environments, and to identify genomic regions controlling heartwood color in C. japonica across multiple environments. Heartwood color in terms of L*a*b* color space and spectral reflectance was measured in common gardens established in three contrasting sites. Quantitative trait loci (QTL) that affect heartwood color were identified using previously constructed highly saturated linkage maps. Results found that heartwood color was largely genetically controlled, and genotype-by-environment interaction explained one-third of the total genetic variance of heartwood color. The effect of the environment was small compared to the effect of genetics, whereas environmental effects largely varied among heartwood color traits. QTL analysis identified a large number of QTLs with small to moderate effects (phenotypic variation explained of 6.6% on average). Some of these QTLs were stably expressed in multiple environments or had pleiotropic effects on heartwood color and moisture content. These results indicated that genetic variation in phenotypic plasticity plays an important role in regulating heartwood color and that the identified QTLs would maximize the breeding efficiency of heartwood color in C. japonica in heterogeneous environments.

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  • Dopamine-related oxidative stress and mitochondrial dysfunction in dopaminergic neurons differentiated from deciduous teeth-derived stem cells of children with Down syndrome

    Xiao Sun, Hiroki Kato, Hiroshi Sato, Xu Han, Yuta Hirofuji, Takahiro A. Kato, Yasunari Sakai, Shouichi Ohga, Satoshi Fukumoto, Keiji Masuda

    FASEB BioAdvances   4 ( 7 )   454 - 467   2022.7   eISSN:2573-9832

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    Down syndrome (DS) is one of the common genetic disorders caused by the trisomy of human chromosome 21 (HSA21). Mitochondrial dysfunction and redox imbalance play important roles in DS pathology, and altered dopaminergic regulation has been demonstrated in the brain of individuals with DS. However, the pathological association of these elements is not yet fully understood. In this study, we analyzed dopaminergic neurons (DNs) differentiated from deciduous teeth-derived stem cells of children with DS or healthy control children. As previously observed in the analysis of a single case of DS, compared to controls, patient-derived DNs (DS-DNs) displayed shorter neurite outgrowth and fewer branches, as well as downregulated vesicular monoamine transporter 2 and upregulated dopamine transporter 1, both of which are key regulators of dopamine homeostasis in DNs. In agreement with these expression profiles, DS-DNs accumulated dopamine intracellularly and had increased levels of cellular and mitochondrial reactive oxygen species (ROS). DS-DNs showed downregulation of non-canonical Notch ligand, delta-like 1, which may contribute to dopamine accumulation and increased ROS levels through DAT1 upregulation. Furthermore, DS-DNs showed mitochondrial dysfunction in consistent with lower expression of peroxisome proliferator-activated receptor-gamma coactivator 1 alpha (PGC-1α) and upregulation of a HSA21-encoded negative regulator of PGC-1α, nuclear receptor-interacting protein 1. These results suggest that dysregulated dopamine homeostasis may participate in oxidative stress and mitochondrial dysfunction of the dopaminergic system in DS.

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  • 左心低形成症候群、完全型DiGeorge症候群、およびプロテインC欠乏症を合併したCHARGE症候群

    坂口 嘉彬, 田中 惇史, 園田 素史, 井上 普介, 石村 匡崇, 永田 弾, 落合 正行, 酒井 康成, 大賀 正一

    日本小児科学会雑誌   126 ( 7 )   1087 - 1087   2022.7   ISSN:0001-6543

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  • Critical vitamin deficiencies in autism spectrum disorder: Reversible and irreversible outcomes. Reviewed International journal

    Pin Fee Chong, Michiko Torio, Fumihiko Fujii, Yuichiro Hirata, Wakato Matsuoka, Yuri Sonoda, Yuko Ichimiya, Yutaro Yada, Noriyuki Kaku, Masataka Ishimura, Momoko Sasazuki, Yuhki Koga, Masafumi Sanefuji, Yasunari Sakai, Shouichi Ohga

    Eur J Clin Nutr   76 ( 11 )   1618 - 1621   2022.6   ISSN:0954-3007 eISSN:1476-5640

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    Vitamin deficiencies are an emerging concern in the management of children with autism spectrum disorder (ASD). Particular attention is required for recognizing the variable signs caused by unbalanced food intakes. We herein report two patients with multiple vitamin deficiencies who needed critical care showing different prognoses. Patient 1 with 'Shoshin' beriberi presenting with cardiac arrest had thiamine deficiency developed severe neurological sequelae despite rapid vitamin supplementation. Patient 2, who had leg pain and a limping gait, showed a rapid recovery with intravenous infusion and tube feeding after being diagnosed with scurvy. A literature search revealed several children with ASD with critically ill thiamine deficiency, but few reports documented a life-threatening condition in the form of cardiac arrest at the onset. Considering the high observation rate of food selectivity in children with ASD, early intervention is required to prevent the exacerbation of vitamin deficiencies to severe neurological disabilities.

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  • Successful induction of human chemically induced liver progenitors with small molecules from damaged liver

    Miyoshi, T; Hidaka, M; Miyamoto, D; Sakai, Y; Murakami, S; Huang, Y; Hara, T; Soyama, A; Kanetaka, K; Ochiya, T; Eguchi, S

    JOURNAL OF GASTROENTEROLOGY   57 ( 6 )   441 - 452   2022.6   ISSN:0944-1174 eISSN:1435-5922

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    Background: Human chemically induced liver progenitors (hCLiP) induced by small molecules produced by mature hepatocytes can potentially overcome issues related to hepatocyte transplantation, such as graft rejection or donor shortage. However, to our knowledge, no studies have explored the induction of hCLiP from mature hepatocytes (MHs) in damaged liver, indicated for liver transplantation. Methods: Liver tissues were collected from surgically resected livers, including damaged livers, of 86 patients at our department, and hepatocytes were isolated using the collagenase perfusion method. Hepatocytes isolated from 33 of these 86 donors were cultured in YAC medium containing Y-27632 (ROCK inhibitor), A-83-01 (TGF-β type I receptor inhibitor), and CHIR99021 (GSK-3 inhibitor) to induce hCLiP, and their functions were assessed. Results: Hepatocytes were isolated regardless of the liver fibrosis classifications (viability: F0,1: 87.2 ± 13.2%; F2,3: 87.8 ± 13.1%; and F4: 86.3 ± 4.2%). Most hepatocytes cultured in the YAC medium acquired the liver progenitor cell (LPC) gene. The expression of MH markers (ALB, HNF4α, G6PC, and CYP1A2) was lower in hCLiP than in MHs before reprogramming. Reverse transcription-polymerase chain reaction revealed that hCLiP markers (e.g., EpCAM, SOX9, CK19, and CD133) exhibited higher expression in LPCs than in MHs. Furthermore, hCLiPs had the ability to differentiate into hepatocytes, and were engrafted on the liver surface as mature hepatocytes. Conclusion: Hepatocytes could be isolated from damaged liver. Furthermore, hCLiP may be obtained from hepatocytes isolated from damaged liver and may differentiate into MHs in vitro. Autologous hCLiP can potentially be transplanted without tumorigenesis and remodel damaged liver.

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  • 神経変性ランゲルハンス細胞組織球症に対する大量免疫グロブリン療法の有効性

    園田 有里, 藤井 史彦, 園田 素史, 石村 匡崇, 一宮 優子, チョン・ピン・フィー, 米元 耕輔, 平良 遼志, 實藤 雅文, 古賀 友紀, 酒井 康成, 大賀 正一

    脳と発達   54 ( Suppl. )   S214 - S214   2022.5   ISSN:0029-0831 eISSN:1884-7668

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  • Promotion of Cyst Formation from a Renal Stem Cell Line Using Organ-Specific Extracellular Matrix Gel Format Culture System

    Sakai, Y; Kubo, Y; Shirakigawa, N; Kawabe, Y; Kamihira, M; Ijima, H

    GELS   8 ( 5 )   2022.5   eISSN:2310-2861

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    Researchers have long awaited the technology to develop an in vitro kidney model. Here, we establish a rapid fabricating technique for kidney-like tissues (cysts) using a combination of an organ-derived extracellular matrix (ECM) gel format culture system and a renal stem cell line (CHK-Q cells). CHK-Q cells, which are spontaneously immortalized from the renal stem cells of the Chinese hamster, formed renal cyst-like structures in a type-I collagen gel sandwich culture on day 1 of culture. The cysts fused together and expanded while maintaining three-dimensional structures. The expression of genes related to kidney development and maturation was increased compared with that in a traditional monolayer. Under the kidney-derived ECM (K-ECM) gel format culture system, cyst formation and maturation were induced rapidly. Gene expressions involved in cell polarities, especially for important material transporters (typical markers Slc5a1 and Kcnj1), were restored. K-ECM composition was an important trigger for CHK-Q cells to promote kidney-like tissue formation and maturation. We have established a renal cyst model which rapidly expressed mature kidney features via the combination of K-ECM gel format culture system and CHK-Q cells.

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  • ヒト誘導ミクログリアを用いた亜急性硬化性全脳炎におけるIL-17シグナル解析

    藤井 史彦, 米元 耕輔, 平良 遼志, 扇谷 昌宏, 加藤 隆弘, 酒井 康成, 大賀 正一

    脳と発達   54 ( Suppl. )   S213 - S213   2022.5   ISSN:0029-0831 eISSN:1884-7668

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  • ヒト誘導ミクログリアにおける細胞不均一性の解析

    米元 耕輔, 藤井 史彦, 平良 遼志, チョン・ピン・フィー, 扇谷 昌宏, 加藤 隆弘, 酒井 康成, 大賀 正一

    脳と発達   54 ( Suppl. )   S268 - S268   2022.5   ISSN:0029-0831 eISSN:1884-7668

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  • First record of Urostylis hubeiensis Ren (Hemiptera, Heteroptera, Urostylididae) from Japan, with an illustrated key to the Japanese urostylidid species

    Souma, J; Sakai, Y; Ishikawa, T

    BIODIVERSITY DATA JOURNAL   10   1 - 15   2022.4   ISSN:1314-2836 eISSN:1314-2828

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    Background Although the Japanese species of Urostylididae are of interest to not only heteropteran taxonomists, but also to the public, an illustrated key for all species of the family from the country is lacking. To date, the urostylidid species Urostylis hubeiensis Ren, 1997, has been known to occur in China and Korea, but not in Japan. New information Urostylis hubeiensis is recorded from Japan for the first time and represents the easternmost occurrence of this species. In Japan, it inhabits the broad-leaved forest of Tsushima Island and was found on Quercus acutissima Carruth. (Fagaceae). An illustrated key to the species of Urostylididae occurring in Japan is provided.

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  • High-dose immunoglobulin therapy for steroid-resistant myositis in juvenile localized scleroderma. Reviewed International journal

    Motoshi Sonoda, Masataka Ishimura, Katsuhide Eguchi, Akira Shiraishi, Yasunari Sakai, Kazunori Urabe, Shouichi Ohga

    Pediatr Neonatol   63 ( 5 )   542 - 544   2022.4   ISSN:1875-9572 eISSN:2212-1692

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  • Bioinspired Perfluorocarbon-Based Oxygen Carriers with Concave Shape and Deformable Shell

    Fu, XT; Ohta, S; Kawakatsu, T; Kamihira, M; Sakai, Y; Ito, T

    ADVANCED MATERIALS TECHNOLOGIES   7 ( 3 )   2022.3   ISSN:2365-709X

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    Human red blood cells (hRBCs) possess a unique biconcave structure with a highly deformable cell membrane and condensed cytosol hemoglobin for oxygen delivery. Inspired by hRBCs, novel deformable core-shell particles are developed as perfluorocarbon-based oxygen carriers (OCs), called “cDFCs” (concave-shaped deformable PFC-based OCs), using the Shirasu porous glass (SPG) membrane emulsification technique. cDFCs have a perfluorooctyl bromide core of high oxygen solubility and poly(lactide-co-caprolactone) shell, which is thin and highly deformable. They have an optical equivalent diameter of 7.9 ± 2.5 µm and a unique concave shape. Owing to their low Young's modulus (93 kPa) and their diameter and shape, they successfully pass through a 4.5-µm-gap silicon microchannel as a blood capillary model. Enhanced oxygen supply to multiple layered cells is demonstrated under hypoxic conditions, indicating their efficiency as OCs. cDFCs are new potential OCs in tissue engineering and blood substitution in the future.

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  • National-scale 3D mapping of soil organic carbon in a Japanese forest considering microtopography and tephra deposition

    Yamashita N., Ishizuka S., Hashimoto S., Ugawa S., Nanko K., Osone Y., Iwahashi J., Sakai Y., Inatomi M., Kawanishi A., Morisada K., Tanaka N., Aizawa S., Imaya A., Takahashi M., Kaneko S., Miura S., Hirai K.

    Geoderma   406   2022.1   ISSN:00167061

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    Geographic information on soil organic carbon (SOC) is important for climate change research and understanding the global carbon cycle. At present, this information is limited for forest soils in mountainous regions that have complex topographies and are affected by tephra deposition. This study predicted the SOC concentration (OC), bulk density (BD), apparent rock coarse fraction (CF), and SOC stock (OCS) at 0–5, 5–15, and 15–30 cm in the soil horizon for the forested areas (ca. 230 000 km<sup>2</sup>) of Japan. This was carried out with a 10 m grid resolution using a digital soil mapping (DSM) approach. To determine the appropriate spatial prediction model, we evaluated the accuracy of a two dimensional approach for ordinary kriging (OK) and regression kriging (RK), and a three dimensional approach for random forest model (RF). The RF was based on topography, climate and vegetation factors (RF<inf>env</inf>), distance and direction from 152 volcanos (RF<inf>env+vol</inf>), distance from survey points (RF<inf>env+sp</inf>); these factors were also combined (RF<inf>all</inf>) as explanatory attributes. The results demonstrated an improvement in the average root mean square errors (RMSEs) of RF<inf>env+vol</inf>, RF<inf>env+sp</inf>, and RF<inf>all</inf> by approximately 12%, 14%, 5%, and 21% for OC, BD, CF, and OCS using 10-fold cross-validation on a site-by-site basis, respectively, compared with OK. Based on the relatively small difference in improvement among RF<inf>env+vol</inf>, RF<inf>env+sp</inf>, and RF<inf>all</inf> (<0.5%), and the considerably high computational cost of RF<inf>env+sp</inf> and RF<inf>all</inf>, RF<inf>env+vol</inf> was considered as the appropriate model for the area. The R<sup>2</sup> of RF<inf>env+vol</inf> was calculated at 0.59, 0.44, 0.30, and 0.38 in OC, BD, CF, and OCS, respectively, using 10-fold cross validation on a site-by-site basis. The predicted map by RF<inf>env+vol</inf> accurately reproduced the large spatial variation in OCS at small watershed, regional, and national scales; this have been derived from the effect of microtopography, tephra deposition, and the gradient of temperature with latitude, respectively. A larger OCS was predicted to accumulate on the ridges, highland slopes, near volcanos, and higher latitudes than at other areas. The mean OCS was estimated to be 7.6 kg·m<sup>−2</sup> for a soil depth of 0–30 cm in Japanese forests. The results also suggest that the fine-scale three dimensional random forest approach using the distances from volcanoes showed promise for regional-scale OCS prediction. This approach was considered particularly effective in hills and mountainous areas that are strongly exposed to tephra deposition, this includes the Pacific Rim regions and other volcanic countries.

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  • Magnetic Ordering and Structural Transition in the Ordered Double-Perovskite Pb<sub>2</sub>NiMoO<sub>6</sub>

    Zhao, JF; Wang, X; Shen, X; Sahle, CJ; Dong, C; Hojo, H; Sakai, Y; Zhang, J; Li, WM; Duan, L; Chan, TS; Chen, CT; Falke, J; Liu, CE; Kuo, CY; Deng, Z; Wang, XC; Yu, RC; Yu, RZ; Hu, ZW; Greenblatt, M; Jin, CQ

    CHEMISTRY OF MATERIALS   34 ( 1 )   97 - 106   2022.1   ISSN:0897-4756 eISSN:1520-5002

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    The B-site-ordered double-perovskite Pb2NiMoO6 was prepared at high pressure and high temperature. The structural analysis of synchrotron powder X-ray diffraction data shows that Pb2NiMoO6 crystallizes into monoclinic symmetry with the space group Pc (no. 7), where the Ni and Mo ions are ordered in a rock-salt-type manner. The magnetic and specific heat characterizations reveal unusual two-step antiferromagnetic (AFM) transitions at 18 and 26 K for Pb2NiMoO6. The X-ray absorption spectra at the Ni-L2,3 edge and the Mo-L3 edge and the high-resolution partial fluorescence yield at the Pb-L3 edge indicate Pb2+2Ni2+Mo6+O6 valence states. Although in A2NiMoO6 (A = Sr2+, Pb2+, and Ba2+), the size of the A cation increases gradually from Sr2+ (1.44 Å) to Pb2+ (1.49 Å) to Ba2+ (1.61 Å), Pb2NiMoO6 exhibits much lower symmetry structure and AFM transition temperature, TN, compared with Sr2NiMoO6 (I4/m, TN = 81 K) and Ba2NiMoO6 (Fm3¯ m, TN = 64 K), which is attributed to the large distortion of NiO6 and MoO6 octahedra induced by the lone pair electron effect of Pb2+ with a 6s2 electronic configuration. Moreover, symmetry-breaking phase transition from a high-temperature centrosymmetric, cubic Fm3¯ m phase to a low-temperature non-centrosymmetric, monoclinic Pc phase was observed at 393-413 K in Pb2NiMoO6.

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  • Impact of a new deep- learning- based reconstruction algorithm on image quality in ultra- high- resolution CT: clinical observational and phantom studies

    Sakai, Y; Hida, T; Matsuura, Y; Kamitani, T; Onizuka, Y; Shirasaka, T; Kato, T; Ishigami, K

    BRITISH JOURNAL OF RADIOLOGY   96 ( 1141 )   20220731   2022.1   ISSN:0007-1285 eISSN:1748-880X

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    Objectives: To demonstrate the effect of an improved deep learning-based reconstruction (DLR) algorithm on Ultra-High-Resolution Computed Tomography (U-HRCT) scanners. Methods: Clinical and phantom studies were conducted. Thirty patients who underwent contrast-enhanced CT examination during the follow-up period were enrolled. Images were reconstructed using improved DLR [termed, New DLR, i.e., Advanced Intelligent Clear-IQ Engine (AiCE) Body Sharp] and conventional DLR (Conv DLR, AiCE Body) algorithms. Two radiologists assessed the overall image quality using a 5-point scale (5 = excellent; 1 = unacceptable). The noise power spectra (NPSs) were calculated to assess the frequency characteristics of the image noise, and the square root of area under the curve (√AUC NPS) between 0.05 and 0.50 cycle/ mm was calculated as an indicator of the image noise. Dunnett’s test was used for statistical analysis of the visual evaluation score, with statistical significance set at p < 0.05. Results: The overall image quality of New DLR was better than that of the Conv DLR (4.2 ± 0.4 and 3.3 ± 0.4, respectively; p < 0.0001). All New DLR images had an overall image quality score above the average or excellent. The √AUC<inf>NPS</inf> value of New DLR was lower than that of Conv DLR (13.8 and 14.2, respectively). The median values of reconstruction time required with New DLR and Conv DLR were 5.0 and 7.8 min, respectively. Conclusions: The new DLR algorithm improved the image quality within a practical reconstruction time. Advances in knowledge: The new DLR enables us to choose whether to improve image quality or reduce the dose.

    DOI: 10.1259/bjr.20220731

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  • The earliest enzyme replacement for infantile-onset Pompe disease in Japan Reviewed International journal

    Vlad Tocan, Yuichi Mushimoto, Kanako Kojima-Ishii, Akane Matsuda, Naoko Toda, Daisuke Toyomura, Yuichiro Hirata, Masafumi Sanefuji, Takaaki Sawada, Yasunari Sakai, Kimitoshi Nakamura, Shouichi Ohga

    Pediatr Int   64 ( 1 )   e15286   2022.1   ISSN:1328-8067 eISSN:1442-200X

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    Background: Infantile-onset Pompe disease (IOPD) is the most severe phenotype of a lysosomal storage disorder caused by acid alpha-glucosidase (GAA) deficiency. An enzymatic newborn screening (NBS) program started regionally in Japan in 2013 for early enzyme replacement therapy (ERT). We report the ERT responses of the first NBS-identified Japanese IOPD case and of another case diagnosed prior to NBS, to discuss the problems of promptly starting ERT in Japan. Methods: Acid alpha-glucosidase activity was measured by fluorometric assay in both patients. The diagnosis of IOPD was confirmed by next-generation followed by Sanger-method sequencing (patient 1) or direct sequencing of polymerase chain reaction (PCR)-amplified products (patient 2) of the GAA gene. Results: A female infant identified by NBS had a novel out-of-frame (p.F181Dfs*6) variant and a reported pathogenic (p.R600C) variant, along with two pseudodeficiency variants. Enzyme replacement therapy was started at age 58 days when the infant had increased serum levels of creatine kinase and slight myocardial hypertrophy. Clinical and biochemical markers improved promptly. She has been alive and well without delayed development at age 14 months. Patient 2, a Japanese male, received a diagnosis of IOPD at age 5 months before the NBS era. He had a homozygotic variant of GAA (p.R608X), later registered as a cross-reactive immunological material (CRIM)-negative genotype, and developed a high titer of anti-rhGAA antibodies. The patient has survived myocardial hypertrophy with continuous respiratory support for 12 years of ERT. Conclusions: Enzyme replacement therapy should not be delayed over the age of 2 months for reversible cardiac function, although CRIM-negative cases may hamper turnaround time reduction.

    DOI: 10.1111/ped.15286

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  • An overview of the first 5 years of the ENIGMA obsessive–compulsive disorder working group: The power of worldwide collaboration

    van den Heuvel O.A., Boedhoe P.S.W., Bertolin S., Bruin W.B., Francks C., Ivanov I., Jahanshad N., Kong X.Z., Kwon J.S., O'Neill J., Paus T., Patel Y., Piras F., Schmaal L., Soriano-Mas C., Spalletta G., van Wingen G.A., Yun J.Y., Vriend C., Simpson H.B., van Rooij D., Hoexter M.Q., Hoogman M., Buitelaar J.K., Arnold P., Beucke J.C., Benedetti F., Bollettini I., Bose A., Brennan B.P., De Nadai A.S., Fitzgerald K., Gruner P., Grünblatt E., Hirano Y., Huyser C., James A., Koch K., Kvale G., Lazaro L., Lochner C., Marsh R., Mataix-Cols D., Morgado P., Nakamae T., Nakao T., Narayanaswamy J.C., Nurmi E., Pittenger C., Reddy Y.C.J., Sato J.R., Soreni N., Stewart S.E., Taylor S.F., Tolin D., Thomopoulos S.I., Veltman D.J., Venkatasubramanian G., Walitza S., Wang Z., Thompson P.M., Stein D.J., Abe Y., Alonso P., Assogna F., Banaj N., Batistuzzo M.C., Brem S., Ciullo V., Feusner J., Martínez-Zalacaín I., Menchón J.M., Miguel E.C., Piacentini J., Piras F., Sakai Y., Wolters L., Yamada K.

    Human Brain Mapping   43 ( 1 )   23 - 36   2022.1   ISSN:10659471

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    Neuroimaging has played an important part in advancing our understanding of the neurobiology of obsessive–compulsive disorder (OCD). At the same time, neuroimaging studies of OCD have had notable limitations, including reliance on relatively small samples. International collaborative efforts to increase statistical power by combining samples from across sites have been bolstered by the ENIGMA consortium; this provides specific technical expertise for conducting multi-site analyses, as well as access to a collaborative community of neuroimaging scientists. In this article, we outline the background to, development of, and initial findings from ENIGMA's OCD working group, which currently consists of 47 samples from 34 institutes in 15 countries on 5 continents, with a total sample of 2,323 OCD patients and 2,325 healthy controls. Initial work has focused on studies of cortical thickness and subcortical volumes, structural connectivity, and brain lateralization in children, adolescents and adults with OCD, also including the study on the commonalities and distinctions across different neurodevelopment disorders. Additional work is ongoing, employing machine learning techniques. Findings to date have contributed to the development of neurobiological models of OCD, have provided an important model of global scientific collaboration, and have had a number of clinical implications. Importantly, our work has shed new light on questions about whether structural and functional alterations found in OCD reflect neurodevelopmental changes, effects of the disease process, or medication impacts. We conclude with a summary of ongoing work by ENIGMA-OCD, and a consideration of future directions for neuroimaging research on OCD within and beyond ENIGMA.

    DOI: 10.1002/hbm.24972

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  • Deep-learning reconstruction for ultra-low-dose lung CT: Volumetric measurement accuracy and reproducibility of artificial ground-glass nodules in a phantom study

    Mikayama, R; Shirasaka, T; Kojima, T; Sakai, Y; Yabuuchi, H; Kondo, M; Kato, T

    BRITISH JOURNAL OF RADIOLOGY   95 ( 1130 )   20210915   2022   ISSN:0007-1285 eISSN:1748-880X

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    Objectives The lung nodule volume determined by CT is used for nodule diagnoses and monitoring tumor responses to therapy. Increased image noise on low-dose CT degrades the measurement accuracy of the lung nodule volume. We compared the volumetric accuracy among deep-learning reconstruction (DLR), model-based iterative reconstruction (MBIR), and hybrid iterative reconstruction (HIR) at an ultra-low-dose setting. Methods Artificial ground-glass nodules (6 mm and 10 mm diameters, −660 HU) placed at the lung-apex and the middle-lung field in chest phantom were scanned by 320-row CT with the ultra-low-dose setting of 6.3 mAs. Each scan data set was reconstructed by DLR, MBIR, and HIR. The volumes of nodules were measured semi-automatically, and the absolute percent volumetric error (APEvol) was calculated. The APEvol provided by each reconstruction were compared by the Tukey-Kramer method. Inter- and intraobserver variabilities were evaluated by a Bland-Altman analysis with limits of agreements. Results DLR provided a lower APEvol compared to MBIR and HIR. The APEvol of DLR (1.36%) was significantly lower than those of the HIR (8.01%, p = 0.0022) and MBIR (7.30%, p = 0.0053) on a 10-mm-diameter middle-lung nodule. DLR showed narrower limits of agreement compared to MBIR and HIR in the inter- and intraobserver agreement of the volumetric measurement. Conclusions DLR showed higher accuracy compared to MBIR and HIR for the volumetric measurement of artificial ground-glass nodules by ultra-low-dose CT. Advances in knowledge DLR with ultra-low-dose setting allows a reduction of dose exposure, maintaining accuracy for the volumetry of lung nodule, especially in patients which deserve a long-term follow-up.

    DOI: 10.1259/bjr.20210915

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  • 日本における乳児発症Pompe病に対する早期酵素補充療法(The earliest enzyme replacement for infantile-onset Pompe disease in Japan)

    Tocan Vlad, Mushimoto Yuichi, Kojima-Ishii Kanako, Matsuda Akane, Toda Naoko, Toyomura Daisuke, Hirata Yuichiro, Sanefuji Masafumi, Sawada Takaaki, Sakai Yasunari, Nakamura Kimitoshi, Ohga Shouichi

    Pediatrics International   64 ( 1 )   ped.15286 - ped.15286   2022   ISSN:1328-8067

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    Language:English   Publisher:John Wiley & Sons Australia, Ltd  

    新生児スクリーニングで同定された日本人初の小児発症Pompe病症例および新生児スクリーニング以前に診断された別症例を対象として、早期酵素補充療法を速やかに開始することの問題点について検討した。症例1は女児で、妊娠36週4日に帝王切開により娩出された。6日目に実施された新生児スクリーニングで酸性アルファグルコシダーゼ(GAA)低値であったため、28日目に当院に紹介された。新生児スクリーニング結果および臨床所見から乳児発症Pompe病が疑われた。GAA遺伝子の次世代シーケンシングの結果、新規アウトフレーム変異(p.F181Dfs*6)および既知変異(p.R600C)が同定され、乳児発症Pompe病と診断された。58日目にERTが開始された。生後58日目にクレアチンキナーゼ血清中濃度が上昇し、心筋肥大がみられたため、酵素補充療法が開始された。その後、臨床的・生化学的マーカーは速やかに改善した。生後14ヵ月で発達遅滞もなく、健常であった。症例2は12歳男児で、NBS時代の前に生後5ヵ月齢で乳児発症Pompe病と診断されていた。GAAホモ接合性変異(p.R608X)が同定された。12年間にわたる酵素補充療法により、心筋肥大は克服された。

  • 第92回日本小児神経学会九州地方会

    酒井 康成

    NO TO HATTATSU   54 ( 3 )   214 - 215   2022   ISSN:00290831 eISSN:18847668

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    Language:Japanese   Publisher:The Japanese Society of Child Neurology  

    DOI: 10.11251/ojjscn.54.214

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  • Progressive B cell depletion in human MALT1 deficiency. Reviewed International journal

    Motoshi Sonoda, Masataka Ishimura, Katsuhide Eguchi, Yutaro Yada, Nina Lenhartová, Akira Shiraishi, Tamami Tanaka, Yasunari Sakai, Shouichi Ohga

    Clin Exp Immunol   206 ( 3 )   237 - 247   2021.12

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    DOI: 10.1111/cei.13662

  • Acute-phase electroencephalography for an infantile atypical teratoid/rhabdoid tumor. Reviewed International journal

    Yuko Ichimiya, Soichi Mizuguchi, Yoshitomo Motomura, Yuhki Koga, Noriyuki Kaku, Nobuhiro Hata, Koji Yoshimoto, Ayumi Sakata, Satoshi O Suzuki, Toru Iwaki, Yasunari Sakai, Shouichi Ohga

    Clin Neurol Neurosurg   209   106922 - 106922   2021.10

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    DOI: 10.1016/j.clineuro.2021.106922

  • Brain-sparing cord blood transplantation for the borderline stage of adrenoleukodystrophy. Reviewed International journal

    Yutaro Yada, Michiko Torio, Yuhki Koga, Fumiya Yamashita, Takuya Ichimura, Katsuhide Eguchi, Masataka Ishimura, Yuichi Mushimoto, Akio Hiwatashi, Momoko Sasazuki, Ryutaro Kira, Yasunari Sakai, Shouichi Ohga

    Mol Genet Metab Rep   28   100778 - 100778   2021.9

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    DOI: 10.1016/j.ymgmr.2021.100778

  • Favorable outcomes of interferon-α and ribavirin treatment for a male with subacute sclerosing panencephalitis. Reviewed International journal

    Yuri Sonoda, Motoshi Sonoda, Kousuke Yonemoto, Masafumi Sanefuji, Ryoji Taira, Yoshitomo Motomura, Masataka Ishimura, Hiroyuki Torisu, Ryutaro Kira, Koichi Kusuhara, Yasunari Sakai, Shouichi Ohga

    J Neuroimmunol   358   577656 - 577656   2021.9

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    DOI: 10.1016/j.jneuroim.2021.577656

  • Age-related morphological differences in the spike-and-wave complexes of absence epilepsy. Reviewed International journal

    Yuri Sonoda, Masafumi Sanefuji, Yuko Ichimiya, Michiko Torio, Eriko Watanabe, Ayumi Sakata, Yoshito Ishizaki, Yasunari Sakai, Shouichi Ohga

    Epilepsy Res   174   106647 - 106647   2021.8

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    DOI: 10.1016/j.eplepsyres.2021.106647

  • Breast feeding and infant development in a cohort with sibling pair analysis: the Japan Environment and Children's Study. Reviewed International journal

    Masafumi Sanefuji, Ayako Senju, Masayuki Shimono, Masanobu Ogawa, Yuri Sonoda, Michiko Torio, Yuko Ichimiya, Reiko Suga, Yasunari Sakai, Satoshi Honjo, Koichi Kusuhara, Shouichi Ohga

    BMJ Open   11 ( 8 )   e043202   2021.8

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    DOI: 10.1136/bmjopen-2020-043202

  • Paroxysmal sympathetic hyperactivity and the later development of epilepsy in a chemotherapy-associated brain damage. Reviewed International journal

    Ryoji Taira, Kenichiro Yamamura, Tomoko Maeda, Ayumi Sakata, Eriko Watanabe, Takafumi Shimogawa, Nobutaka Mukae, Chizuru Ikeda, Masahiro Migita, Osamu Watanabe, Yuhki Koga, Yasunari Sakai, Shouichi Ohga

    Brain Dev   2021.7

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    DOI: 10.1016/j.braindev.2021.07.001

  • Clarifying the Pathophysiological Mechanisms of Neuronal Abnormalities of NF1 by Induced-Neuronal (iN) Cells from Human Fibroblasts Reviewed International journal

    Noriaki Sagata, Yasunari Sakai, Takahiro A. Kato

    Neuropsychopharm Rep   2021.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.5772/intechopen.98817

  • A nation-wide survey of Japanese pediatric MOG antibody-associated diseases. Reviewed International journal

    Kohji Azumagawa, Ichiro Nakashima, Kimihiko Kaneko, Hiroyuki Torisu, Yasunari Sakai, Ryutaro Kira, Hiroshi Sakuma, Keiko Tanaka, Yasushi Shigeri, Yoshie Tanaka, Hideto Nakajima, Shuichi Shimakawa, Hiroshi Tamai

    Brain Dev   43 ( 6 )   705 - 713   2021.6

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    DOI: 10.1016/j.braindev.2021.01.008

  • Predictive values of early head computed tomography for survival outcome after cardiac arrest in childhood: a pilot study. Reviewed International journal

    Kenichi Tetsuhara, Noriyuki Kaku, Yuka Watanabe, Masaya Kumamoto, Yuko Ichimiya, Soichi Mizuguchi, Kanako Higashi, Wakato Matsuoka, Yoshitomo Motomura, Masafumi Sanefuji, Akio Hiwatashi, Yasunari Sakai, Shouichi Ohga

    Sci Rep   11 ( 1 )   12090 - 12090   2021.6

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    DOI: 10.1038/s41598-021-91628-y

  • Alectinib-responsive infantile anaplastic ganglioglioma with a novel VCL-ALK gene fusion. Reviewed International journal

    Shunsuke Yamamoto, Yuhki Koga, Hiroaki Ono, Hironori Goto, Nobuhiro Hata, Hidetaka Yamamoto, Satoshi O Suzuki, Yasunari Sakai, Toru Iwaki, Shouichi Ohga

    Pediatr Blood Cancer   68 ( 9 )   e29122   2021.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/pbc.29122

  • Clinical manifestations and epilepsy treatment in Japanese patients with pathogenic CDKL5 variants. Reviewed International journal

    Yu Kobayashi, Jun Tohyama, Yukitoshi Takahashi, Tomohide Goto, Kazuhiro Haginoya, Takeshi Inoue, Masaya Kubota, Hiroshi Fujita, Ryoko Honda, Masahiro Ito, Kanako Kishimoto, Kazuyuki Nakamura, Yasunari Sakai, Jun-Ichi Takanashi, Manabu Tanaka, Koichi Tanda, Koji Tominaga, Seiichiro Yoshioka, Mitsuhiro Kato, Mitsuko Nakashima, Hirotomo Saitsu, Naomichi Matsumoto

    Brain Dev   43 ( 4 )   505 - 514   2021.4

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    DOI: 10.1016/j.braindev.2020.12.006

  • Vitamin A deficiency-associated corneal perforation in a boy with autism spectrum disorder: A case report and literature review. Reviewed International journal

    Shunichi Adachi, Michiko Torio, Sayaka Okuzono, Yoshitomo Motomura, Yuko Ichimiya, Yuri Sonoda, Jyunya Nagata, Misato Okamoto, Shouji Noutomi, Masafumi Sanefuji, Yasunari Sakai, Shouichi Ohga

    Nutrition   90   111275 - 111275   2021.4

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    DOI: 10.1016/j.nut.2021.111275

  • Assessment of Pediatric Admissions for Kawasaki Disease or Infectious Disease During the COVID-19 State of Emergency in Japan. Reviewed International journal

    Takuya Hara, Kenji Furuno, Kenichiro Yamamura, Junji Kishimoto, Yumi Mizuno, Kenji Murata, Sagano Onoyama, Ken Hatae, Megumi Takemoto, Yoshito Ishizaki, Shunsuke Kanno, Kazuo Sato, Yoshitomo Motomura, Yasunari Sakai, Shouichi Ohga, Mayumi Yashiro, Yoshikazu Nakamura, Toshiro Hara

    JAMA Netw Open   4 ( 4 )   e214475   2021.4

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    DOI: 10.1001/jamanetworkopen.2021.4475

  • De novo ATP1A3 variants cause polymicrogyria. Reviewed International journal

    Satoko Miyatake, Mitsuhiro Kato, Takuma Kumamoto, Tomonori Hirose, Eriko Koshimizu, Takaaki Matsui, Hideyuki Takeuchi, Hiroshi Doi, Keisuke Hamada, Mitsuko Nakashima, Kazunori Sasaki, Akio Yamashita, Atsushi Takata, Kohei Hamanaka, Mai Satoh, Takabumi Miyama, Yuri Sonoda, Momoko Sasazuki, Hiroyuki Torisu, Toshiro Hara, Yasunari Sakai, Yushi Noguchi, Mazumi Miura, Yoko Nishimura, Kazuyuki Nakamura, Hideyuki Asai, Nodoka Hinokuma, Fuyuki Miya, Tatsuhiko Tsunoda, Masami Togawa, Yukihiro Ikeda, Nobusuke Kimura, Kaoru Amemiya, Asako Horino, Masataka Fukuoka, Hiroko Ikeda, Goni Merhav, Nina Ekhilevitch, Masaki Miura, Takeshi Mizuguchi, Noriko Miyake, Atsushi Suzuki, Shouichi Ohga, Hirotomo Saitsu, Hidehisa Takahashi, Fumiaki Tanaka, Kazuhiro Ogata, Chiaki Ohtaka-Maruyama, Naomichi Matsumoto

    Sci Adv   7 ( 13 )   2021.3

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    DOI: 10.1126/sciadv.abd2368

  • Forskolin rapidly enhances neuron-like morphological change of directly induced-neuronal cells from neurofibromatosis type 1 patients. Reviewed International journal

    Noriaki Sagata, Shin-Ichi Kano, Masahiro Ohgidani, Shogo Inamine, Yasunari Sakai, Hiroki Kato, Keiji Masuda, Takeshi Nakahara, Makiko Nakahara-Kido, Shouichi Ohga, Masutaka Furue, Akira Sawa, Shigenobu Kanba, Takahiro A Kato

    Neuropsychopharm Rep   40 ( 4 )   396 - 400   2020.12

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    DOI: 10.1002/npr2.12144

  • De novo CACNA1G variants in developmental delay and early-onset epileptic encephalopathies. Reviewed International journal

    Misako Kunii, Hiroshi Doi, Shunta Hashiguchi, Toyojiro Matsuishi, Yasunari Sakai, Mizue Iai, Masaki Okubo, Haruko Nakamura, Keita Takahashi, Atsuko Katsumoto, Mikiko Tada, Hideyuki Takeuchi, Taro Ishikawa, Noriko Miyake, Hirotomo Saitsu, Naomichi Matsumoto, Fumiaki Tanaka

    J Neurol Sci   416   117047 - 117047   2020.9

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    DOI: 10.1016/j.jns.2020.117047

  • Parvovirus B19-Infected Tubulointerstitial Nephritis in Hereditary Spherocytosis. Reviewed International journal

    Kei Nishiyama, Yuka Watanabe, Masataka Ishimura, Kenichi Tetsuhara, Takashi Imai, Hikaru Kanemasa, Kenji Ueki, Yoshitomo Motomura, Noriyuki Kaku, Yasunari Sakai, Ken-Ichi Imadome, Shouichi Ohga

    Open Forum Infect Dis   7 ( 8 )   ofaa288   2020.8

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    DOI: 10.1093/ofid/ofaa288

  • Management of apnea in infants with trisomy 18. Reviewed International journal

    Ryoji Taira, Hirosuke Inoue, Toru Sawano, Junko Fujiyoshi, Yuko Ichimiya, Michiko Torio, Masafumi Sanefuji, Masayuki Ochiai, Yasunari Sakai, Shouichi Ohga

    Dev Med Child Neurol   62 ( 7 )   874 - 878   2020.7

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    DOI: 10.1111/dmcn.14403

  • The expanding phenotype of hypokalemic periodic paralysis in a Japanese family with p.Val876Glu mutation in CACNA1S. Reviewed International journal

    Mari Kurokawa, Michiko Torio, Kazuhiro Ohkubo, Vlad Tocan, Noriko Ohyama, Naoko Toda, Kanako Ishii, Kei Nishiyama, Yuichi Mushimoto, Ryuichi Sakamoto, Maki Nakaza, Riho Horie, Tomoya Kubota, Masanori P Takahashi, Yasunari Sakai, Masatoshi Nomura, Shouichi Ohga

    Mol Genet Genomic Med   8 ( 4 )   e1175   2020.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/mgg3.1175

  • Gastrointestinal symptoms as an extended clinical feature of Pierson syndrome: a case report and review of the literature. Reviewed International journal

    Kei Nishiyama, Mari Kurokawa, Michiko Torio, Yasunari Sakai, Mitsuru Arima, Shoko Tsukamoto, Satoshi Obata, Shogo Minamikawa, Kandai Nozu, Noriyuki Kaku, Yoshihiko Maehara, Koh-Hei Sonoda, Tomoaki Taguchi, Shouichi Ohga

    BMC Med Genet   21 ( 1 )   80 - 80   2020.4

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    DOI: 10.1186/s12881-020-01019-9

  • De novo p.G696S mutation in COL4A1 causes intracranial calcification and late-onset cerebral hemorrhage: A case report and review of the literature. Reviewed International journal

    Keishiro Kinoshita, Yoshito Ishizaki, Hiroyuki Yamamoto, Motoshi Sonoda, Kousuke Yonemoto, Ryutaro Kira, Masafumi Sanefuji, Akihiko Ueda, Hirotaka Matsui, Yukio Ando, Yasunari Sakai, Shouichi Ohga

    Eur J Med Genet   63 ( 4 )   103825 - 103825   2020.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.ejmg.2019.103825

  • Survival and ocular preservation in a long-term cohort of Japanese patients with retinoblastoma. Reviewed International journal

    Tamaki Ueda, Yuhki Koga, Hiroshi Yoshikawa, Mika Tanabe, Kanako Yamana, Utako Oba, Kentaro Nakashima, Hiroaki Ono, Takuya Ichimura, Shunji Hasegawa, Wakako Kato, Tetsuko Kobayashi, Hideki Nakayama, Yasunari Sakai, Tadamasa Yoshitake, Saiji Ohga, Yoshinao Oda, Shigenobu Suzuki, Koh-Hei Sonoda, Shouichi Ohga

    BMC Pediatr   20 ( 1 )   37 - 37   2020.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1186/s12887-020-1923-7

  • Prognostic factors for survival of herpes simplex virus-associated hemophagocytic lymphohistiocytosis. Reviewed

    Motoshi Sonoda, Masataka Ishimura, Katsuhide Eguchi, Akira Shiraishi, Shunsuke Kanno, Noriyuki Kaku, Hirosuke Inoue, Yoshitomo Motomura, Masayuki Ochiai, Yasunari Sakai, Manabu Nakayama, Osamu Ohara, Shouichi Ohga

    Int J Hematol   111 ( 1 )   131 - 136   2020.1

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    DOI: 10.1007/s12185-019-02738-3

  • Association of perinatal factors of epilepsy in very low birth weight infants, using a nationwide database in Japan. Reviewed International journal

    Yuki Matsushita, Yasunari Sakai, Michiko Torio, Hirosuke Inoue, Masayuki Ochiai, Kazuaki Yasuoka, Hiroaki Kurata, Junko Fujiyoshi, Masako Ichiyama, Tomoaki Taguchi, Kiyoko Kato, Shouichi Ohga

    J Perinatol   39 ( 11 )   1472 - 1479   2019.11

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    DOI: 10.1038/s41372-019-0494-7

  • Late-Onset Circulatory Collapse and Risk of Cerebral Palsy in Extremely Preterm Infants. Reviewed International journal

    Kazuaki Yasuoka, Hirosuke Inoue, Naoki Egami, Masayuki Ochiai, Koichi Tanaka, Toru Sawano, Hiroaki Kurata, Masako Ichiyama, Junko Fujiyoshi, Yuki Matsushita, Yasunari Sakai, Shouichi Ohga

    J Pediatr   212   117 - 123   2019.9

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    DOI: 10.1016/j.jpeds.2019.05.033

  • Decision-making dilemmas of paediatricians: a qualitative study in Japan. Reviewed International journal

    Momoko Sasazuki, Yasunari Sakai, Ryutaro Kira, Naoko Toda, Yuko Ichimiya, Satoshi Akamine, Michiko Torio, Yoshito Ishizaki, Masafumi Sanefuji, Miho Narama, Koichiro Itai, Toshiro Hara, Hidetoshi Takada, Yoshiyuki Kizawa, Shouichi Ohga

    BMJ Open   9 ( 8 )   e026579   2019.8

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    DOI: 10.1136/bmjopen-2018-026579

  • Recurrent de novo MAPK8IP3 variants cause neurological phenotypes. Reviewed International journal

    Shinya Iwasawa, Kumiko Yanagi, Atsuo Kikuchi, Yasuko Kobayashi, Kazuhiro Haginoya, Hiroshi Matsumoto, Kenji Kurosawa, Masayuki Ochiai, Yasunari Sakai, Atsushi Fujita, Noriko Miyake, Tetsuya Niihori, Matsuyuki Shirota, Ryo Funayama, Shigeaki Nonoyama, Shouichi Ohga, Hiroshi Kawame, Keiko Nakayama, Yoko Aoki, Naomichi Matsumoto, Tadashi Kaname, Yoichi Matsubara, Wataru Shoji, Shigeo Kure

    Ann Neuol   85 ( 6 )   927 - 933   2019.6

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    DOI: 10.1002/ana.25481

  • Comprehensive analysis of coding variants highlights genetic complexity in developmental and epileptic encephalopathy. Reviewed International journal

    Atsushi Takata, Mitsuko Nakashima, Hirotomo Saitsu, Takeshi Mizuguchi, Satomi Mitsuhashi, Yukitoshi Takahashi, Nobuhiko Okamoto, Hitoshi Osaka, Kazuyuki Nakamura, Jun Tohyama, Kazuhiro Haginoya, Saoko Takeshita, Ichiro Kuki, Tohru Okanishi, Tomohide Goto, Masayuki Sasaki, Yasunari Sakai, Noriko Miyake, Satoko Miyatake, Naomi Tsuchida, Kazuhiro Iwama, Gaku Minase, Futoshi Sekiguchi, Atsushi Fujita, Eri Imagawa, Eriko Koshimizu, Yuri Uchiyama, Kohei Hamanaka, Chihiro Ohba, Toshiyuki Itai, Hiromi Aoi, Ken Saida, Tomohiro Sakaguchi, Kouhei Den, Rina Takahashi, Hiroko Ikeda, Tokito Yamaguchi, Kazuki Tsukamoto, Shinsaku Yoshitomi, Taikan Oboshi, Katsumi Imai, Tomokazu Kimizu, Yu Kobayashi, Masaya Kubota, Hirofumi Kashii, Shimpei Baba, Mizue Iai, Ryutaro Kira, Munetsugu Hara, Masayasu Ohta, Yohane Miyata, Rie Miyata, Jun-Ichi Takanashi, Jun Matsui, Kenji Yokochi, Masayuki Shimono, Masano Amamoto, Rumiko Takayama, Shinichi Hirabayashi, Kaori Aiba, Hiroshi Matsumoto, Shin Nabatame, Takashi Shiihara, Mitsuhiro Kato, Naomichi Matsumoto

    Nat Commun   10 ( 1 )   2506 - 2506   2019.6

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    DOI: 10.1038/s41467-019-10482-9

  • Positive effect of exogenous brain-derived neurotrophic factor on impaired neurite development and mitochondrial function in dopaminergic neurons derived from dental pulp stem cells from children with attention deficit hyperactivity disorder. Reviewed International journal

    Huong Thi Nguyen Nguyen, Hiroki Kato, Hiroshi Sato, Haruyoshi Yamaza, Yasunari Sakai, Shouichi Ohga, Kazuaki Nonaka, Keiji Masuda

    Biochem Bbiophysical Res Commun   513 ( 4 )   1048 - 1054   2019.6

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    DOI: 10.1016/j.bbrc.2019.04.084

  • A Child with Prostaglandin I2-associated Thyrotoxicosis: Case Report Reviewed International journal

    Yuri Sonoda, Kenichiro Yamamura, Kanako Ishii, Kazuhiro Ohkubo, Kenji Ihara, Yasunari Sakai, Shouichi Ohga

    J Clin Res Pediatr Endocrinol   11 ( 2 )   207 - 210   2019.5

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    DOI: 10.4274/jcrpe.galenos.2018.2018.0169

  • A Nationwide Survey of Pediatric-onset Japanese Encephalitis in Japan. Reviewed International journal

    Etsuro Nanishi, Takayuki Hoshina, Masafumi Sanefuji, Ryo Kadoya, Katsuhiko Kitazawa, Yukie Arahata, Tetsuya Sato, Yoshimichi Hirayama, Katsuki Hirai, Masaaki Yanai, Kaori Nikaido, Akihiko Maeda, Hiroyuki Torisu, Kenji Okada, Yasunari Sakai, Shouichi Ohga

    Clin Infectious Dis   68 ( 12 )   2099 - 2104   2019.5

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    DOI: 10.1093/cid/ciy816

  • Late-onset sepsis and encephalopathy after bicycle-spoke injury: a case report. Reviewed International journal

    Ryuichi Takemoto, Yoshitomo Motomura, Noriyuki Kaku, Yuko Ichimiya, Mamoru Muraoka, Shunsuke Kanno, Tamami Tanaka, Yasunari Sakai, Yoshihiko Maehara, Shouichi Ohga

    BMC Infect Dis   19 ( 1 )   472 - 472   2019.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1186/s12879-019-4082-4

  • An acute encephalopathy with reduced diffusion in BRAF-associated cardio-facio-cutaneous syndrome. Reviewed International journal

    Sayaka Okuzono, Ryoko Fukai, Marie Noda, Noriko Miyake, Sooyoung Lee, Noriyuki Kaku, Masafumi Sanefuji, Satoshi Akamine, Shunsuke Kanno, Yoshito Ishizaki, Hiroyuki Torisu, Ryutaro Kira, Naomichi Matsumoto, Yasunari Sakai, Shouichi Ohga

    Brain Dev   41 ( 4 )   378 - 381   2019.4

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    DOI: 10.1016/j.braindev.2018.10.012

  • Early Intervention With Adrenocorticotropin for Acute Encephalopathy-Associated Epileptic Spasms: Report of Two Cases. Reviewed International journal

    Kousuke Yonemoto, Yuko Ichimiya, Masafumi Sanefuji, Noriyuki Kaku, Ayumi Sakata, Rieko Baba, Fumiya Yamashita, Satoshi Akamine, Michiko Torio, Yoshito Ishizaki, Yoshihiko Maehara, Yasunari Sakai, Shouichi Ohga

    Clin EEG Neurosci   50 ( 1 )   51 - 55   2019.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/1550059418786381

  • A nationwide survey on tracheostomy for very-low-birth-weight infants in Japan. Reviewed International journal

    Hiroaki Kurata, Masayuki Ochiai, Hirosuke Inoue, Masako Ichiyama, Kazuaki Yasuoka, Junko Fujiyoshi, Yuki Matsushita, Satoshi Honjo, Yasunari Sakai, Shouichi Ohga

    Pediatr Pulmonol   54 ( 1 )   53 - 60   2019.1

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    DOI: 10.1002/ppul.24200

  • Vascular pathomechanism in acute encephalopathy with biphasic seizures and late reduced diffusion. Reviewed International journal

    Masafumi Sanefuji, Yuko Ichimiya, Noriyuki Kaku, Momoko Sasazuki, Kosuke Yonemoto, Michiko Torio, Soichi Mizuguchi, Yoshitomo Motomura, Mamoru Muraoka, Sooyoung Lee, Haruhisa Baba, Kazuhiro Ohkubo, Yuri Sonoda, Yoshito Ishizaki, Yasunari Sakai, Shouichi Ohga

    J Neurol Sci   395   141 - 146   2018.12

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    DOI: 10.1016/j.jns.2018.10.007

  • Neurodevelopmental Outcomes in Infants With Birth Weight ≤500 g at 3 Years of Age. Reviewed International journal

    Hirosuke Inoue, Masayuki Ochiai, Yasunari Sakai, Kazuaki Yasuoka, Koichi Tanaka, Masako Ichiyama, Hiroaki Kurata, Junko Fujiyoshi, Yuki Matsushita, Satoshi Honjo, Kazuaki Nonaka, Tomoaki Taguchi, Kiyoko Kato, Shouichi Ohga

    Pediatrics   142 ( 6 )   2018.12

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    DOI: 10.1542/peds.2017-4286

  • Impaired neurite development associated with mitochondrial dysfunction in dopaminergic neurons differentiated from exfoliated deciduous tooth-derived pulp stem cells of children with autism spectrum disorder. Reviewed International journal

    Huong Thi Nguyen Nguyen, Hiroki Kato, Keiji Masuda, Haruyoshi Yamaza, Yuta Hirofuji, Hiroshi Sato, Thanh Thi Mai Pham, Fumiko Takayama, Yasunari Sakai, Shouichi Ohga, Tomoaki Taguchi, Kazuaki Nonaka

    Biochem Biophys Rep   16   24 - 31   2018.12

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    DOI: 10.1016/j.bbrep.2018.09.004

  • Radiotherapy for Langerhans cell histiocytosis with paraplegia: A rare oncologic emergency case report in infancy and literature review. Reviewed International journal

    Kentaro Nakashima, Yuhki Koga, Yasunari Sakai, Hidetoshi Takada, Katsumi Harimaya, Saiji Ohga, Tomoaki Taguchi, Yoshinao Oda, Hiroshi Honda, Shouichi Ohga

    Brain Dev   40 ( 10 )   952 - 955   2018.11

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    DOI: 10.1016/j.braindev.2018.05.016

  • Diagnostic potential of stored dried blood spots for inborn errors of metabolism: a metabolic autopsy of medium-chain acyl-CoA dehydrogenase deficiency. Reviewed International journal

    Noriyuki Kaku, Kenji Ihara, Yuichiro Hirata, Kenji Yamada, Sooyoung Lee, Hikaru Kanemasa, Yoshitomo Motomura, Haruhisa Baba, Tamami Tanaka, Yasunari Sakai, Yoshihiko Maehara, Shouichi Ohga

    J Clin Pathol   71 ( 10 )   885 - 889   2018.10

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    DOI: 10.1136/jclinpath-2017-204962

  • Leucine-rich alpha-2 glycoprotein in the cerebrospinal fluid is a potential inflammatory biomarker for meningitis. Reviewed International journal

    Pin Fee Chong, Yasunari Sakai, Hiroyuki Torisu, Tamami Tanaka, Kenji Furuno, Yumi Mizuno, Shouichi Ohga, Toshiro Hara, Ryutaro Kira

    J Neurol Sci   392   51 - 55   2018.9

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    DOI: 10.1016/j.jns.2018.07.006

  • A male case with CDKL5-associated encephalopathy manifesting transient methylmalonic acidemia. Reviewed International journal

    Satoshi Akamine, Yoshito Ishizaki, Yasunari Sakai, Hiroyuki Torisu, Ryoko Fukai, Noriko Miyake, Kazuhiro Ohkubo, Hiroshi Koga, Masafumi Sanefuji, Ayumi Sakata, Masahiko Kimura, Seiji Yamaguchi, Osamu Sakamoto, Toshiro Hara, Hirotomo Saitsu, Naomichi Matsumoto, Shouichi Ohga

    Eur J Med Genet   61 ( 8 )   451 - 454   2018.8

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    DOI: 10.1016/j.ejmg.2018.03.003

  • Atypical erythroblastosis in a patient with Diamond-Blackfan anemia who developed del(20q) myelodysplasia. Reviewed

    Motoshi Sonoda, Masataka Ishimura, Yuko Ichimiya, Eiko Terashi, Katsuhide Eguchi, Yasunari Sakai, Hidetoshi Takada, Asahito Hama, Hitoshi Kanno, Tsutomu Toki, Etsuro Ito, Shouichi Ohga

    Int J Hematol   108 ( 2 )   228 - 231   2018.8

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    DOI: 10.1007/s12185-018-2424-4

  • Correction to: Atypical erythroblastosis in a patient with Diamond-Blackfan anemia who developed del(20q) myelodysplasia. Reviewed

    Motoshi Sonoda, Masataka Ishimura, Yuko Ichimiya, Eiko Terashi, Katsuhide Eguchi, Yasunari Sakai, Hidetoshi Takada, Asahito Hama, Hitoshi Kanno, Tsutomu Toki, Etsuro Ito, Shouichi Ohga

    Int J Hematol   108 ( 2 )   236 - 236   2018.8

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    DOI: 10.1007/s12185-018-2493-4

  • Deletions of SCN2A and SCN3A genes in a patient with West syndrome and autistic spectrum disorder. Reviewed International journal

    Pin Fee Chong, Hirotomo Saitsu, Yasunari Sakai, Toru Imagi, Ryoko Nakamura, Masaru Matsukura, Naomichi Matsumoto, Ryutaro Kira

    Seizure   60   91 - 93   2018.8

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    DOI: 10.1016/j.seizure.2018.06.012

  • Predictive indicators for the development of epilepsy after acute encephalopathy with biphasic seizures and late reduced diffusion. Reviewed International journal

    Yuko Ichimiya, Noriyuki Kaku, Masafumi Sanefuji, Michiko Torio, Soichi Mizuguchi, Yoshitomo Motomura, Mamoru Muraoka, Sooyoung Lee, Haruhisa Baba, Yuri Sonoda, Yoshito Ishizaki, Momoko Sasazuki, Yasunari Sakai, Yoshihiko Maehara, Shouichi Ohga

    Epilepsy Res   143   70 - 74   2018.7

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    DOI: 10.1016/j.eplepsyres.2018.04.006

  • Cancer Management in Kabuki Syndrome: The First Case of Wilms Tumor and a Literature Review. Reviewed International journal

    Hideto Teranishi, Yuhki Koga, Kentaro Nakashima, Eiji Morihana, Kanako Ishii, Yasunari Sakai, Tomoaki Taguchi, Yoshinao Oda, Noriko Miyake, Naomichi Matsumoto, Shouichi Ohga

    J Pediatr Hematol Oncol   40 ( 5 )   391 - 394   2018.7

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    DOI: 10.1097/MPH.0000000000001111

  • A rightward saccade to an unexpected stimulus as a marker for lateralised visuospatial attention. Reviewed International journal

    Masafumi Sanefuji, Hiroshi Yamashita, Michiko Torio, Daisuke Katsuki, Satoshi Akamine, Yoshito Ishizaki, Junji Kishimoto, Yasunari Sakai, Hidetoshi Takada, Keiko Yoshida, Shouichi Ohga

    Sci Rep   8 ( 1 )   7562 - 7562   2018.5

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    DOI: 10.1038/s41598-018-25890-y

  • Mitochondrial dysfunction in dopaminergic neurons differentiated from exfoliated deciduous tooth-derived pulp stem cells of a child with Rett syndrome. Reviewed International journal

    Saki Hirofuji, Yuta Hirofuji, Hiroki Kato, Keiji Masuda, Haruyoshi Yamaza, Hiroshi Sato, Fumiko Takayama, Michiko Torio, Yasunari Sakai, Shouichi Ohga, Tomoaki Taguchi, Kazuaki Nonaka

    Biochem Biophys Res Commun   498 ( 4 )   898 - 904   2018.4

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    DOI: 10.1016/j.bbrc.2018.03.077

  • Effective shunt closure for pulmonary hypertension and liver dysfunction in congenital portosystemic venous shunt. Reviewed International journal

    Kiyoshi Uike, Hazumu Nagata, Yuichiro Hirata, Kenichiro Yamamura, Eiko Terashi, Toshiharu Matsuura, Eiji Morihana, Kazuhiro Ohkubo, Kanako Ishii, Yasunari Sakai, Tomoaki Taguchi, Shouichi Ohga

    Pediatr Pulmonol   53 ( 4 )   505 - 511   2018.4

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    DOI: 10.1002/ppul.23944

  • Early-onset epileptic encephalopathy and severe developmental delay in an association with de novo double mutations in NF1 and MAGEL2. Reviewed International journal

    Satoshi Akamine, Noriaki Sagata, Yasunari Sakai, Takahiro A Kato, Takeshi Nakahara, Yuki Matsushita, Osamu Togao, Akio Hiwatashi, Masafumi Sanefuji, Yoshito Ishizaki, Hiroyuki Torisu, Hirotomo Saitsu, Naomichi Matsumoto, Toshiro Hara, Akira Sawa, Shinichi Kano, Masutaka Furue, Shigenobu Kanba, Chad A Shaw, Shouichi Ohga

    Epilepsia Open   3 ( 1 )   81 - 85   2018.3

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    DOI: 10.1002/epi4.12085

  • Integrative Analyses of De Novo Mutations Provide Deeper Biological Insights into Autism Spectrum Disorder. Reviewed International journal

    Atsushi Takata, Noriko Miyake, Yoshinori Tsurusaki, Ryoko Fukai, Satoko Miyatake, Eriko Koshimizu, Itaru Kushima, Takashi Okada, Mako Morikawa, Yota Uno, Kanako Ishizuka, Kazuhiko Nakamura, Masatsugu Tsujii, Takeo Yoshikawa, Tomoko Toyota, Nobuhiko Okamoto, Yoko Hiraki, Ryota Hashimoto, Yuka Yasuda, Shinji Saitoh, Kei Ohashi, Yasunari Sakai, Shouichi Ohga, Toshiro Hara, Mitsuhiro Kato, Kazuyuki Nakamura, Aiko Ito, Chizuru Seiwa, Emi Shirahata, Hitoshi Osaka, Ayumi Matsumoto, Saoko Takeshita, Jun Tohyama, Tomoko Saikusa, Toyojiro Matsuishi, Takumi Nakamura, Takashi Tsuboi, Tadafumi Kato, Toshifumi Suzuki, Hirotomo Saitsu, Mitsuko Nakashima, Takeshi Mizuguchi, Fumiaki Tanaka, Norio Mori, Norio Ozaki, Naomichi Matsumoto

    Cell Rep   22 ( 3 )   734 - 747   2018.1

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    DOI: 10.1016/j.celrep.2017.12.074

  • Sustained endocrine profiles of a girl with WAGR syndrome. Reviewed International journal

    Yui Takada, Yasunari Sakai, Yuki Matsushita, Kazuhiro Ohkubo, Yuhki Koga, Satoshi Akamine, Michiko Torio, Yoshito Ishizaki, Masafumi Sanefuji, Hiroyuki Torisu, Chad A Shaw, Masayo Kagami, Toshiro Hara, Shouichi Ohga

    BMC Med Genet   18 ( 1 )   117 - 117   2017.10

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    DOI: 10.1186/s12881-017-0477-5

  • Calcineurin inhibitors exacerbate coronary arteritis via the MyD88 signalling pathway in a murine model of Kawasaki disease Reviewed International journal

    K. Murata, Y. Motomura, T. Tanaka, S. Kanno, T. Yano, M. Onimaru, A. Shimoyama, H. Nishio, Y. Sakai, M. Oh-Hora, H. Hara, K. Fukase, H. Takada, S. Masuda, S. Ohga, S. Yamasaki, T. Hara

    Clin Exp Immunol   190 ( 1 )   54 - 67   2017.10

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    DOI: 10.1111/cei.13002

  • Dysregulated gene expressions of MEX3D, FOS and BCL2 in human induced-neuronal (iN) cells from NF1 patients: a pilot study. Reviewed International journal

    Noriaki Sagata, Takahiro A Kato, Shin-Ichi Kano, Masahiro Ohgidani, Norihiro Shimokawa, Mina Sato-Kasai, Kohei Hayakawa, Nobuki Kuwano, Ashley M Wilson, Koko Ishizuka, Shiori Kato, Takeshi Nakahara, Makiko Nakahara-Kido, Daiki Setoyama, Yasunari Sakai, Shouichi Ohga, Masutaka Furue, Akira Sawa, Shigenobu Kanba

    Sci Rep   7 ( 1 )   13905 - 13905   2017.10

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    DOI: 10.1038/s41598-017-14440-7

  • Disappearance of globus pallidum lesions in T1-weighted magnetic resonance images after ligation of congenital portosystemic venous shunt. Reviewed International journal

    Ryuichi Takemoto, Kenichiro Yamamura, Hazumu Nagata, Naoki Kawaguchi, Yasunari Sakai, Toshiharu Matsuura, Tomoaki Taguchi, Shouichi Ohga

    Pediatri Neonatol   58 ( 5 )   465 - 466   2017.10

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    DOI: 10.1016/j.pedneo.2017.05.001

  • A childhood-onset intestinal toxemia botulism during chemotherapy for relapsed acute leukemia. Reviewed International journal

    Noriko Ohyama, Michiko Torio, Kentaro Nakashima, Yuuki Koga, Shunsuke Kanno, Hisanori Nishio, Kei Nishiyama, Momoko Sasazuki, Haru Kato, Hiroshi Asakura, Satoshi Akamine, Masafumi Sanefuji, Yoshito Ishizaki, Yasunari Sakai, Shouichi Ohga

    Ann Clin Microbiol Antimicrob   16 ( 1 )   61 - 61   2017.9

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    DOI: 10.1186/s12941-017-0240-y

  • Transient dysautonomia in an acute phase of encephalopathy with biphasic seizures and late reduced diffusion. Reviewed International journal

    Yuko Ichimiya, Noriyuki Kaku, Yasunari Sakai, Fumiya Yamashita, Wakato Matsuoka, Mamoru Muraoka, Satoshi Akamine, Soichi Mizuguchi, Michiko Torio, Yoshitomo Motomura, Yuichiro Hirata, Yoshito Ishizaki, Masafumi Sanefuji, Hiroyuki Torisu, Hidetoshi Takada, Yoshihiko Maehara, Shouichi Ohga

    Brain Dev   39 ( 7 )   621 - 624   2017.8

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    DOI: 10.1016/j.braindev.2017.03.023

  • Mutations in genes encoding polycomb repressive complex 2 subunits cause Weaver syndrome. Reviewed International journal

    Eri Imagawa, Ken Higashimoto, Yasunari Sakai, Chikahiko Numakura, Nobuhiko Okamoto, Satoko Matsunaga, Akihide Ryo, Yoshinori Sato, Masafumi Sanefuji, Kenji Ihara, Yui Takada, Gen Nishimura, Hirotomo Saitsu, Takeshi Mizuguchi, Satoko Miyatake, Mitsuko Nakashima, Noriko Miyake, Hidenobu Soejima, Naomichi Matsumoto

    Hum Mutat   38 ( 6 )   637 - 648   2017.6

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    DOI: 10.1002/humu.23200

  • A nationwide survey of pediatric acquired demyelinating syndromes in Japan. Reviewed International journal

    Yamaguchi Y, Torisu H, Kira R, Ishizaki Y, Sakai Y, Sanefuji M, Ichiyama T, Oka A, Kishi T, Kimura S, Kubota M, Takanashi J, Takahashi Y, Tamai H, Natsume J, Hamano S, Hirabayashi S, Maegaki Y, Mizuguchi M, Minagawa K, Yoshikawa H, Kira J, Kusunoki S, Hara T

    Neurology   87 ( 19 )   2006 - 2015   2016.11

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    A nationwide survey of pediatric acquired demyelinating syndromes in Japan.

    DOI: 10.1212/WNL.0000000000003318

  • Involuntary movements and coma as the prognostic marker for acute encephalopathy with biphasic seizures and late reduced diffusion. Reviewed International journal

    Lee S, Sanefuji M, Torio M, Kaku N, Ichimiya Y, Mizuguchi S, Baba H, Sakai Y, Ishizaki Y, Torisu H, Kira R, Hara T, Ohga S

    J Neurol Sci   370   39 - 43   2016.11

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    Involuntary movements and coma as the prognostic marker for acute encephalopathy with biphasic seizures and late reduced diffusion.
    Acute encephalopathy with biphasic seizures and late reduced diffusion (AESD) occurs in children associated with infection. It is characterized by a prolonged febrile seizure in the first phase, and a cluster of seizures, deterioration of consciousness and the white matter lesions with reduced diffusion in the second phase. The patients often have severe neurological sequelae, but the prognostic indicators remain unknown. The present study aimed to clarify the characteristics of AESD patients who subsequently exhibited severe neurological sequelae. We retrospectively analyzed the clinical and laboratory findings along with the brain imaging in patients who had severe (n=8) and non-severe neurodevelopmental outcomes (n=12). Severe group more frequently showed coma (p=0.014) or involuntary movements including dystonia and oral dyskinesia (p=0.018) before the second phase than non-severe group. Severe group exhibited higher levels of serum alanine aminotransferase than non-severe group (p=0.001). Quantitatively assessed MRI in the second phase revealed that severe group had more extensive lesions than non-severe group, in the anterior (p=0.015) and posterior parts (p=0.011) of the cerebrum and basal ganglia (p=0.020). Early appearing involuntary movements or coma might account for the extension of acute brain lesions and the poor neurological outcomes in AESD patients.

    DOI: 10.1016/j.jns.2016.09.018

  • Periodic Epileptiform Discharges in Children With Advanced Stages of Progressive Myoclonic Epilepsy. Reviewed International journal

    Natsumi Isobe, Yasunari Sakai, Ryutaro Kira, Masafumi Sanefuji, Yoshito Ishizaki, Ayumi Sakata, Momoko Sasazuki, Michiko Torio, Satoshi Akamine, Hiroyuki Torisu, Toshiro Hara

    Clin EEG Neurosci   47 ( 4 )   317 - 323   2016.10

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    DOI: 10.1177/1550059415579767

  • De novo p.Arg756Cys mutation of ATP1A3 causes an atypical form of alternating hemiplegia of childhood with prolonged paralysis and choreoathetosis. Reviewed International journal

    Kanemasa H, Fukai R, Sakai Y, Torio M, Miyake N, Lee S, Ono H, Akamine S, Nishiyama K, Sanefuji M, Ishizaki Y, Torisu H, Saitsu H, Matsumoto N, Hara T

    BMC Neurol   16   174 - 174   2016.9

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    De novo p.Arg756Cys mutation of ATP1A3 causes an atypical form of alternating hemiplegia of childhood with prolonged paralysis and choreoathetosis.
    BACKGROUND: Alternating hemiplegia of childhood (AHC) is a rare neurological disorder that manifests recurrent attacks of hemiplegia, oculogyric, and choreoathetotic involuntary movements. De novo mutations in ATP1A3 cause three types of neurological diseases: AHC; rapid-onset dystonia-Parkinsonism (RDP); and cerebellar ataxia, areflexia, pes cavus, optic atrophy, and sensorineural hearing loss (CAPOS) syndromes. It remains to be determined whether or not a rare mutation in ATP1A3 may cause atypical phenotypes. CASE PRESENTATION: A 7-year-old boy presented with recurrent symptoms of generalized paralysis since 1 year and 5 months of age. Hypotonia, dystonia, and choreoathetosis persisted with exacerbation under febrile conditions, but no cerebellar ataxia had ever evolved in 6 years. Whole-exome sequencing (WES) was performed to determine his genetic background, and mutations were validated by the Sanger method. Crude protein extracts were prepared from the cultured cells, and expression of the wild-type or mutant ATP1A3 proteins were analyzed by Western blotting. WES identified a de novo pathogenic mutation in ATP1A3 (c.2266C > T:p.R756C) for this patient. A literature overview of two reported cases with p.R756C and p.R756H mutations showed both overlapping and distinct phenotypes when compared with those of the present case. The expression of the mutant form (R756C) of ATP1A3 did not differ markedly from that of the wild-type and D801N proteins. CONCLUSIONS: This study confirmed that p.R756C mutation of ATP1A3 cause atypical forms of AHC-associated disorders. The wide spectra of neurological phenotypes in AHC are linked to as-yet-unknown deficits in the functions of mutant ATP1A3.

    DOI: 10.1186/s12883-016-0680-6

  • Corrigendum: Hyperactive mTOR signals in the proopiomelanocortin-expressing hippocampal neurons cause age-dependent epilepsy and premature death in mice. Reviewed International journal

    Yuki Matsushita, Yasunari Sakai, Mitsunori Shimmura, Hiroshi Shigeto, Miki Nishio, Satoshi Akamine, Masafumi Sanefuji, Yoshito Ishizaki, Hiroyuki Torisu, Yusaku Nakabeppu, Akira Suzuki, Hidetoshi Takada, Toshiro Hara

    Sci Rep   6   27164 - 27164   2016.6

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    DOI: 10.1038/srep27164

  • De novo KCNH1 mutations in four patients with syndromic developmental delay, hypotonia and seizures Reviewed International journal

    Ryoko Fukai, Hirotomo Saitsu, Yoshinori Tsurusaki, Yasunari Sakai, Kazuhiro Haginoya, Kazumasa Takahashi, Monika Weisz Hubshman, Nobuhiko Okamoto, Mitsuko Nakashima, Fumiaki Tanaka, Noriko Miyake, Naomichi Matsumoto

    J Immunol   61 ( 5 )   381 - 387   2016.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/jhg.2016.1

  • Identifying diagnostically-relevant resting state brain functional connectivity in the ventral posterior complex via genetic data mining in autism spectrum disorder Reviewed International journal

    Philip R. Baldwin, Kaylah N. Curtis, Michelle A. Patriquin, Varina Wolf, Humsini Viswanath, Chad Shaw, Yasunari Sakai, Ramiro Salas

    Autism Res   9 ( 5 )   553 - 562   2016.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/aur.1559

  • De novo missense mutations in NALCN cause developmental and intellectual impairment with hypotonia Reviewed International journal

    Ryoko Fukai, Hirotomo Saitsu, Nobuhiko Okamoto, Yasunari Sakai, Aviva Fattal-Valevski, Shiina Masaaki, Yukihiro Kitai, Michiko Torio, Kanako Kojima-Ishii, Kenji Ihara, Veronika Chernuha, Mitsuko Nakashima, Satoko Miyatake, Fumiaki Tanaka, Noriko Miyake, Naomichi Matsumoto

    J Hum Genet   61 ( 5 )   451 - 455   2016.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/jhg.2015.163

  • Activation of Nod1 Signaling Induces Fetal Growth Restriction and Death through Fetal and Maternal Vasculopathy Reviewed International journal

    Hirosuke Inoue, Hisanori Nishio, Hidetoshi Takada, Yasunari Sakai, Etsuro Nanishi, Masayuki Ochiai, Mitsuho Onimaru, Si Jing Chen, Toshiro Matsui, Toshiro Hara

    J Immunol   196 ( 6 )   2779 - 2787   2016.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.4049/jimmunol.1500295

  • Phenotypic spectrum of GNAO1 variants: Epileptic encephalopathy to involuntary movements with severe developmental delay Reviewed International journal

    Hirotomo Saitsu, Ryoko Fukai, Bruria Ben-Zeev, Yasunari Sakai, Masakazu Mimaki, Nobuhiko Okamoto, Yasuhiro Suzuki, Yukifumi Monden, Hiroshi Saito, Barak Tziperman, Michiko Torio, Satoshi Akamine, Nagahisa Takahashi, Hitoshi Osaka, Takanori Yamagata, Kazuyuki Nakamura, Yoshinori Tsurusaki, Mitsuko Nakashima, Noriko Miyake, Masaaki Shiina, Kazuhiro Ogata, Naomichi Matsumoto

    Eur J Hum Genet   24 ( 1 )   129 - 134   2016.1

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    DOI: 10.1038/ejhg.2015.92

  • Moyamoya disease susceptibility gene RNF213 links inflammatory and angiogenic signals in endothelial cells. Reviewed International journal

    Ohkubo K, Sakai Y, Inoue H, Akamine S, Ishizaki Y, Matsushita Y, Sanefuji M, Torisu H, Ihara K, Sardiello M, Hara T

    Sci Rep   5   13191 - 13191   2015.8

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    Moyamoya disease susceptibility gene RNF213 links inflammatory and angiogenic signals in endothelial cells.
    Moyamoya disease (MMD) is a cerebrovascular disorder characterized by occlusive lesions of the circle of Willis. To date, both environmental and genetic factors have been implicated for pathogenesis of MMD. Allelic variations in RNF213 are known to confer the risk of MMD; however, functional roles of RNF213 remain to be largely elusive. We herein report that pro-inflammatory cytokines, IFNG and TNFA, synergistically activated transcription of RNF213 both in vitro and in vivo. Using various chemical inhibitors, we found that AKT and PKR pathways contributed to the transcriptional activation of RNF213. Transcriptome-wide analysis and subsequent validation with quantitative PCR supported that endogenous expression of cell cycle-promoting genes were significantly decreased with knockdown of RNF213 in cultured endothelial cells. Consistently, these cells showed less proliferative and less angiogenic profiles. Chemical inhibitors for AKT (LY294002) and PKR (C16) disrupted their angiogenic potentials, suggesting that RNF213 and its upstream pathways cooperatively organize the process of angiogenesis. Furthermore, RNF213 down-regulated expressions of matrix metalloproteases in endothelial cells, but not in fibroblasts or other cell types. Altogether, our data illustrate that RNF213 plays unique roles in endothelial cells for proper gene expressions in response to inflammatory signals from environments.

    DOI: 10.1038/srep13191

  • A case of pontine tegmental cap dysplasia with comorbidity of oculoauriculovertebral spectrum. Reviewed International journal

    Chong PF, Haraguchi K, Torio M, Kirino M, Ogata R, Matsukura M, Sakai Y, Ishizaki Y, Yamamoto T, Kira R

    Brain Dev   37 ( 1 )   171 - 174   2015.1

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    A case of pontine tegmental cap dysplasia with comorbidity of oculoauriculovertebral spectrum.

    DOI: 10.1016/j.braindev.2014.02.007

  • Kawasaki Disease-Specific Molecules in the Sera Are Linked to Microbe-Associated Molecular Patterns in the Biofilms Reviewed International journal

    Takeshi Kusuda, Yasutaka Nakashima, Kenji Murata, Shunsuke Kanno, Hisanori Nishio, Mitsumasa Saito, Tamami Tanaka, Kenichiro Yamamura, Yasunari Sakai, Hidetoshi Takada, Tomofumi Miyamoto, Yumi Mizuno, Kazunobu Ouchi, Kenji Waki, Toshiro Hara

    PLoS One   9 ( 11 )   2014.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pone.0113054

  • Altered strategy in short-term memory for pictures in children with attention-deficit/hyperactivity disorder: A near-infrared spectroscopy study Reviewed International journal

    Masafumi Sanefuji, Hiroshi Yamashita, Hiroyuki Torisu, Yui Takada, Hisako Imanaga, Mayumi Matsunaga, Yoshito Ishizaki, Yasunari Sakai, Keiko Yoshida, Toshiro Hara

    Psychiatr Res   223 ( 1 )   37 - 42   2014.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.pscychresns.2014.04.012

  • Testicular sex cord-stromal tumor in a boy with 2q37 deletion syndrome Reviewed International journal

    Yasunari Sakai, Ryota Souzaki, Hidetaka Yamamoto, Yuki Matsushita, Hazumu Nagata, Yoshito Ishizaki, Hiroyuki Torisu, Yoshinao Oda, Tomoaki Taguchi, Chad A. Shaw, Toshiro Hara

    BMC Med Genomics   7   2014.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1186/1755-8794-7-19

  • Girl with a PRRT2 mutation and infantile focal epilepsy with bilateral spikes Reviewed International journal

    Hiroyuki Torisu, Kyoko Watanabe, Keiko Shimojima, Midori Sugawara, Masafumi Sanefuji, Yoshito Ishizaki, Yasunari Sakai, Hironori Yamashita, Toshiyuki Yamamoto, Toshiro Hara

    Brain Dev   36 ( 4 )   342 - 345   2014.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.braindev.2013.05.009

  • Neuroendocrine phenotypes in a boy with 5q14 deletion syndrome implicate the regulatory roles of myocyte-specific enhancer factor 2C in the postnatal hypothalamus. Reviewed International journal

    Sakai Y, Ohkubo K, Matsushita Y, Akamine S, Ishizaki Y, Torisu H, Ihara K, Sanefuji M, Kim MS, Lee KU, Shaw CA, Lim J, Nakabeppu Y, Hara T

    Eur J Med Genet   56 ( 9 )   475 - 483   2013.9

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    Neuroendocrine phenotypes in a boy with 5q14 deletion syndrome implicate the regulatory roles of myocyte-specific enhancer factor 2C in the postnatal hypothalamus.

    DOI: 10.1016/j.ejmg.2013.06.009

  • Parental age and child growth and development: Child health check-up data Reviewed International journal

    Mariko Iwayama, Ryutaro Kira, Naoko Kinukawa, Yasunari Sakai, Hiroyuki Torisu, Masafumi Sanefuji, Yoshito Ishizaki, Yoshiaki Nose, Toshimichi Matsumoto, Toshiro Hara

    Pediatr Int   53 ( 5 )   709 - 714   2011.10

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    DOI: 10.1111/j.1442-200X.2011.03331.x

  • Oligogenic heterozygosity in individuals with high-functioning autism spectrum disorders Reviewed International journal

    Christian P. Schaaf, Aniko Sabo, Yasunari Sakai, Jacy Crosby, Donna Muzny, Alicia Hawes, Lora Lewis, Humeira Akbar, Robin Varghese, Eric Boerwinkle, Richard A. Gibbs, Huda Y. Zoghbi

    Hum Mol Genet   20 ( 17 )   3366 - 3375   2011.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/hmg/ddr243

  • Parental ages and the growth and development of children: a study from check-ups. Reviewed International journal

    Iwayama M, Kira R, Kinukawa N, Sakai Y, Torisu H, Sanefuji M, Ishizaki Y, Nose Y, Matsumoto T, Hara T

    Pediatr Int   2011.2

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    Parental ages and the growth and development of children: a study from check-ups.

    DOI: 10.1111/j.1442-200X.2011.03331.x

  • PD1 as a common candidate susceptibility gene of subacute sclerosing panencephalitis Reviewed International journal

    Yoshito Ishizaki, Naoko Yukaya, Koichi Kusuhara, Ryutaro Kira, Hiroyuki Torisu, Kenji Ihara, Yasunari Sakai, Masafumi Sanefuji, Judy R. Pipo-Deveza, Catherine Lynn T. Silao, Benilda C. Sanchez, Marissa B. Lukban, Aida M. Salonga, Toshiro Hara

    Hum Genet   127 ( 4 )   411 - 419   2010.4

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    DOI: 10.1007/s00439-009-0781-z

  • Genetic susceptibility to febrile seizures: Case-control association studies Reviewed International journal

    Ryutaro Kira, Yoshito Ishizaki, Hiroyuki Torisu, Masafumi Sanefuji, Megumi Takemoto, Kanji Sakamoto, Shigetaka Matsumoto, Yui Yamaguchi, Naoko Yukaya, Yasunari Sakai, Kenjiro Gondo, Toshiro Hara

    Brain Dev   32 ( 1 )   57 - 63   2010.1

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    DOI: 10.1016/j.braindev.2009.09.018

  • Interleukin-10 is associated with resistance to febrile seizures: Genetic association and experimental animal studies Reviewed International journal

    Yoshito Ishizaki, Ryutaro Kira, Mitsumasa Fukuda, Hiroyuki Torisu, Yasunari Sakai, Masafumi Sanefuji, Naoko Yukaya, Toshiro Hara

    Epilepsia   50 ( 4 )   761 - 767   2009.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/j.1528-1167.2008.01861.x

  • Association of toll-like receptor 3 gene polymorphism with subacute sclerosing panencephalitis Reviewed International journal

    Yoshito Ishizaki, Megumi Takemoto, Ryutaro Kira, Koichi Kusuhara, Hiroyuki Torisu, Yasunari Sakai, Masafumi Sanefuji, Naoko Yukaya, Toshiro Hara

    J Neurovirol   14 ( 6 )   486 - 491   2008.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/13550280802298120

  • The relationship between retrieval success and task performance during the recognition of meaningless shapes: An event-related near-infrared spectroscopy study Reviewed International journal

    Masafumi Sanefuji, Taisuke Nakashima, Ryutaro Kira, Mariko Iwayama, Hiroyuki Torisu, Yasunari Sakai, Toshiro Hara

    Neurosci Res   59 ( 2 )   191 - 198   2007.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.neures.2007.06.1480

  • The GT to GC single nucleotide polymorphism at the beginning of an alternative exon 2C of human MTH1 gene confers an amino terminal extension that functions as a mitochondrial targeting signal Reviewed International journal

    Yasunari Sakai, Hisanobu Oda, Daisuke Yoshimura, Masato Furuichi, Dongchon Kang, Shigenori Iwai, Toshiro Hara, Yusaku Nakabeppu

    J Mol Med   84 ( 8 )   660 - 670   2006.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00109-006-0053-5

  • A novel R275X mutation of the SLC25A15 gene in a Japanese patient with the HHH syndrome. Reviewed International journal

    Torisu H, Kira R, Kanazawa N, Takemoto M, Sanefuji M, Sakai Y, Tsujino S, Hara T

    Brain Dev   28 ( 5 )   332 - 335   2006.6

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    A novel R275X mutation of the SLC25A15 gene in a Japanese patient with the HHH syndrome.

    DOI: 10.1016/j.braindev.2005.10.002

  • Benign convulsion with mild gastroenteritis and benign familial infantile seizure. Reviewed International journal

    Sakai Y, Kira R, Torisu H, Yasumoto S, Saito M, Kusuhara K, Hara T

    Epilepsy Res   68 ( 3 )   269 - 271   2006.3

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    Benign convulsion with mild gastroenteritis and benign familial infantile seizure.

    DOI: 10.1016/j.eplepsyres.2006.01.004

  • A novel transfection method for mammalian cells using gas plasma Reviewed International journal

    Yasunari Sakai, Vahid Khajoee, Yasuhiro Ogawa, Koichi Kusuhara, Yoshiki Katayama, Toshiro Hara

    J Biotechnol   121 ( 3 )   299 - 308   2006.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jbiotec.2005.08.020

  • Genetic susceptibility to simple febrile seizures: interleukin-1beta promoter polymorphisms are associated with sporadic cases. Reviewed International journal

    Kira R, Torisu H, Takemoto M, Nomura A, Sakai Y, Sanefuji M, Sakamoto K, Matsumoto S, Gondo K, Hara T

    Neurosci Lett   384   239 - 244   2005.8

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    Genetic susceptibility to simple febrile seizures: interleukin-1beta promoter polymorphisms are associated with sporadic cases.

    DOI: 10.1016/j.neulet.2005.04.097

  • Gene expression profiles in peripheral blood mononuclear cells from patients with subacute sclerosing panencephalitis using oligonucleotide microarrays. Reviewed International journal

    Takemoto M, Kira R, Kusuhara K, Torisu H, Sakai Y, Hara T

    J Neurovirol   11   299 - 305   2005.7

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    Gene expression profiles in peripheral blood mononuclear cells from patients with subacute sclerosing panencephalitis using oligonucleotide microarrays.

    DOI: 10.1080/13550280590953825

  • Structure of human MTH1, a Nudix family hydrolase that selectively degrades oxidized purine nucleoside triphosphates. Reviewed International journal

    Mishima M, Sakai Y, Itoh N, Kamiya H, Furuichi M, Takahashi M, Yamagata Y, Iwai S, Nakabeppu Y, Shirakawa M

    J Biol Chem   279 ( 32 )   33806 - 33815   2004.8

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    Structure of human MTH1, a Nudix family hydrolase that selectively degrades oxidized purine nucleoside triphosphates.

    DOI: 10.1074/jbc.M402393200

  • Functional MxA promoter polymorphism associated with subacute sclerosing panencephalitis. Reviewed International journal

    Torisu H, Kusuhara K, Kira R, Bassuny WM, Sakai Y, Sanefuji M, Takemoto M, Hara T

    Neurology   62   457 - 460   2004.2

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    Functional MxA promoter polymorphism associated with subacute sclerosing panencephalitis.

  • An oxidized purine nucleoside triphosphatase, MTH1, suppresses cell death caused by oxidative stress. Reviewed International journal

    Yoshimura D, Sakumi K, Ohno M, Sakai Y, Furuichi M, Iwai S, Nakabeppu Y

    J Biol Chem   278 ( 39 )   37965 - 37973   2003.9

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    An oxidized purine nucleoside triphosphatase, MTH1, suppresses cell death caused by oxidative stress.

    DOI: 10.1074/jbc.M306201200

  • Founder effect of the C9 R95X mutation in Orientals. Reviewed International journal

    Khajoee V, Ihara K, Kira R, Takemoto M, Torisu H, Sakai Y, Guanjun J, Hee PM, Tokunaga K, Hara T

    Hum Genet   112   244 - 248   2003.3

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    Founder effect of the C9 R95X mutation in Orientals.

    DOI: 10.1007/s00439-002-0870-8

  • Role of tryptophan residues in the recognition of mutagenic oxidized nucleotides by human antimutator MTH1 protein. Reviewed International journal

    Masayuki Takahashi, Fabrice Maraboeuf, Yasunari Sakai, Hiroyuki Yakushiji, Masaki Mishima, Masahiro Shirakawa, Shigenori Iwai, Hiroshi Hayakawa, Mutsuo Sekiguchi, Yusaku Nakabeppu

    J Mol Biology   319 ( 1 )   129 - 39   2002.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0022-2836(02)00163-8

  • Human APE2 protein is mostly localized in the nuclei and to some extent in the mitochondria, while nuclear APE2 is partly associated with proliferating cell nuclear antigen International journal

    Daisuke Tsuchimoto, Daisuke Tsuchimoto, Yasunari Sakai, Yasunari Sakai, Kunihiko Sakumi, Kunihiko Sakumi, Kenichi Nishioka, Kenichi Nishioka, Masafumi Sasaki, Toshiyuki Fujiwara, Yusaku Nakabeppu, Yusaku Nakabeppu, Yusaku Nakabeppu

    Nucleic Acids Res   29   2349 - 2360   2001.6

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    Human APE2 protein is mostly localized in the nuclei and to some extent in the mitochondria, while nuclear APE2 is partly associated with proliferating cell nuclear antigen.
    In human cells APE1 is the major AP endonuclease and it has been reported to have no functional mitochondrial targeting sequence (MTS). We found that APE2 protein possesses a putative MTS. When its N-terminal 15 amino acid residues were fused to the N-terminus of green fluorescent protein and transiently expressed in HeLa cells the fusion protein was localized in the mitochondria. By electron microscopic immunocytochemistry we detected authentic APE2 protein in mitochondria from HeLa cells. Western blotting of the subcellular fraction of HeLa cells revealed most of the APE2 protein to be localized in the nuclei. We found a putative proliferating cell nuclear antigen (PCNA)-binding motif in the C-terminal region of APE2 and showed this motif to be functional by immunoprecipitation and in vitro pull-down binding assays. Laser scanning immunofluorescence microscopy of HeLa cells demonstrated both APE2 and PCNA to form foci in the nucleus and also to be co-localized in some of the foci. The incubation of HeLa cells in HAT medium containing deoxyuridine significantly increased the number of foci in which both molecules were co-localized. Our results suggest that APE2 participates in both nuclear and mitochondrial BER and also that nuclear APE2 functions in the PCNA-dependent BER pathway.

    DOI: 10.1093/nar/29.11.2349

  • Intracellular Distribution of the Antimutagenic Enzyme MTH1 in the Liver, Kidney, and Testis of F344 Rats and its Modulation by Cadmium. International journal

    K. S. Kasprzak, Y. Nakabeppu, Y. Nakabeppu, T. Kakuma, Y. Sakai, Y. Sakai, K. Tsuruya, M. Sekiguchi, J. M. Ward, B. A. Diwan, K. Nagashima, B. H. Kasprzak

    Exp Toxicol Pathol   53(5), 325-336   325 - 335   2001.1

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    Intracellular distribution of the antimutagenic enzyme MTH1 in the liver, kidney and testis of F344 rats and its modulation by cadmium
    Cellular distribution of the antimutagenic MTH1 protein in the liver, kidney, and testis of Fischer rat was evaluated using the immunohistochemical staining with anti-MTH1 polyclonal antibody. The present investigation revealed a non-uniform distribution of MTH1 among cells and among the cytoplasmic, nuclear, and membranal structures of cells within a given tissue. A particularly strong expression of MTH1 was observed for the first time in the perinuclear acrosomic bodies of spermatocytes and in the acrosomic vesicles of sperm heads. Treatment of rats with a single sc dose of 20 μmol Cd(II)/kg body wt. produced histopathologic changes in these organs accompanied by redistribution of the cellular MTH1 protein between the cytoplasm and nuclei. The acute phase of Cd(II) toxicity, that in the liver and especially in the testes (but not in kidneys) led to cell necrosis, was accompanied by a characteristic decrease in the abundance of MTH1-expressing nuclei. Chronic toxicity without necrosis, persisting in the kidney over the entire 14-day study, as well as the survival and proliferation of cells, observed in the liver and testis after the necrotizing phase, were signified by increased number of nuclei expressing MTH1. Thus, unlike previous biochemical studies, immunohistochemistry managed to reveal alterations in the patterns of inter- and intracellular distribution of MTH1, associated apparently with the conditional changes in the dynamics of synthesis of nucleic acids, assisted by this protein.

    DOI: 10.1078/0940-2993-00201

  • Trisomy 10 in a child with acute nonlymphocytic leukemia followed by relapse with a different clone Reviewed International journal

    Yasunari Sakai, Hideki Nakayama, Akinobu Matsuzaki, Yoshihisa Nagatoshi, Aiko Suminoe, Keiko Honda, Takeshi Inamitsu, Shouichi Ohga, Toshiro Hara

    Cancer Genet Cytogenet   115 ( 1 )   47 - 51   1999.11

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    DOI: 10.1016/S0165-4608(99)00087-4

  • Interferon-α therapy for chronic active Epstein-Barr virus infection: Potential effect on the development of T-lymphoproliferative disease Reviewed International journal

    Yasunari Sakai, Shouichi Ohga, Yasuhiko Tonegawa, Hidetoshi Takada, Futoshi Nakao, Hideki Nakayama, Tomonobu Aoki, Shunji Yamamori, Toshiro Hara

    J Pediatr Hematol Oncolog   20 ( 4 )   342 - 346   1998.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1097/00043426-199807000-00013

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MISC

  • 【超急性期におけるリハビリテーション診療マニュアル】重症循環機能障害に対するリハビリテーション治療と安全管理

    山本 周平, 酒井 康成, 木村 和広

    MEDICAL REHABILITATION   ( 313 )   65 - 76   2025.5   ISSN:1346-0773

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    Language:Japanese   Publisher:(株)全日本病院出版会  

    日本循環器学会が主導するDPCデータベース(JROAD-DPC)の分析によれば,心不全患者における高齢化の進行および日常生活動作(ADL)の低下が顕著であり,集中治療室(ICU)への入室患者数が増加傾向にあることが示されている.一方で,入院中の心臓リハビリテーション実施率は55.8%と漸増しており,その重要性が広く認識されつつある.また,近年のMCS(機械的循環補助)の進展は,ICUにおける重症患者の救命率向上に大きく寄与している一方で,新たなリハビリテーションの課題を浮き彫りにしている.特に,ECMO(体外式膜型人工肺)やIMPELLA(経皮的心室補助装置)などのデバイスを装着している患者では,ADLの著しい低下やICU獲得性筋力低下(ICU-AW)が生じやすく,これらに対する早期のリハビリテーション介入の重要性が高まっている.従来のガイドラインにおいては,IABP(大動脈内バルーンパンピング)やECMO管理下でのリハビリテーションは禁忌とされていた.しかし,2023年に改訂された「重症患者リハビリテーション診療ガイドライン」においては,V-V ECMO装着患者の離床は禁忌ではなくなった.さらに,central ECMO管理下での歩行練習や,IMPELLA装着下での歩行練習に関する症例報告が出るなど,リハビリテーションの適応範囲は大きく拡大している.MCS装置を装着した患者では,低灌流や全身性炎症反応が原因となり骨格筋萎縮,脳血流の低下,腸管機能障害が引き起こされることが多く,これらがADLのさらなる低下を招く要因となる.特に,ECMOを導入した患者の約37%が6ヵ月後に身体機能,精神機能,認知機能に障害を抱えるとの報告があり,早期のリハビリテーション介入がその予後改善において重要であると示唆されている.こうした背景から,ICUから段階的かつ計画的にリハビリテーションを進めることは,退院時のADL向上や再入院リスクの低減に寄与すると考えられ,ICU患者の長期的な予後改善にもつながる可能性が高いと考えられる.(著者抄録)

  • だれも取り残さない小児神経診療にむけて 地域格差はあるのか? オンライン診療の活用

    加賀 佳美, 赤坂 真奈美, 岡崎 伸, 野崎 拓朗, 本島 敏乃, 酒井 康成, 高橋 悟, 平田 佑子

    脳と発達   57 ( 2 )   91 - 101   2025.3   ISSN:0029-0831

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    Language:Japanese   Publisher:(一社)日本小児神経学会  

  • 【子どもの心と育ちを理解するツール】小児科医の目からみた遠城寺式乳幼児分析的発達検査の有用性と課題

    市山 正子, 川上 沙織, 井上 普介, 吉良 龍太郎, 酒井 康成, 大賀 正一

    教育と医学   72 ( 5 )   418 - 426   2024.9   ISSN:0452-9677

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    Language:Japanese   Publisher:慶應義塾大学出版会(株)  

  • 【公費補助制度を使いこなす!】多発性硬化症

    梶原 健太, チョン・ピンフィー , 酒井 康成

    小児科診療   87 ( 8 )   1080 - 1084   2024.8   ISSN:0386-9806

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    Language:Japanese   Publisher:(株)診断と治療社  

    <文献概要>▽多発性硬化症は,時間的および空間的に多発する中枢神経の炎症性脱髄性病変を特徴とする自己免疫性神経疾患である.▽多発性硬化症は小児期から発症し得る疾患であり,急性期の治療だけでなく,再発および進行防止のための治療や慢性期の対症療法やリハビリテーションなどの治療継続が,生涯にわたり必要となる.▽本稿では,成人期の予後を含めた疾患の概要のほか,わが国での多発性硬化症に対する医療費助成や公的支援について述べる.

  • Correction to: White matter diffusion estimates in obsessive-compulsive disorder across 1653 individuals: machine learning findings from the ENIGMA OCD Working Group (Molecular Psychiatry, (2024), 29, 4, (1063-1074), 10.1038/s41380-023-02392-6)

    Kim B.G., Kim G., Abe Y., Alonso P., Ameis S., Anticevic A., Arnold P.D., Balachander S., Banaj N., Bargalló N., Batistuzzo M.C., Benedetti F., Bertolín S., Beucke J.C., Bollettini I., Brem S., Brennan B.P., Buitelaar J.K., Calvo R., Castelo-Branco M., Cheng Y., Chhatkuli R.B., Ciullo V., Coelho A., Couto B., Dallaspezia S., Ely B.A., Ferreira S., Fontaine M., Fouche J.P., Grazioplene R., Gruner P., Hagen K., Hansen B., Hanna G.L., Hirano Y., Höxter M.Q., Hough M., Hu H., Huyser C., Ikuta T., Jahanshad N., James A., Jaspers-Fayer F., Kasprzak S., Kathmann N., Kaufmann C., Kim M., Koch K., Kvale G., Kwon J.S., Lazaro L., Lee J., Lochner C., Lu J., Manrique D.R., Martínez-Zalacaín I., Masuda Y., Matsumoto K., Maziero M.P., Menchón J.M., Minuzzi L., Moreira P.S., Morgado P., Narayanaswamy J.C., Narumoto J., Ortiz A.E., Ota J., Pariente J.C., Perriello C., Picó-Pérez M., Pittenger C., Poletti S., Real E., Reddy Y.C.J., van Rooij D., Sakai Y., Sato J.R., Segalas C., Shavitt R.G., Shen Z., Shimizu E., Shivakumar V., Soreni N., Soriano-Mas C., Sousa N., Sousa M.M., Spalletta G., Stern E.R., Stewart S.E., Szeszko P.R., Thomas R., Thomopoulos S.I., Vecchio D., Venkatasubramanian G., Vriend C., Walitza S., Wang Z., Watanabe A., Wolters L.

    Molecular Psychiatry   29 ( 4 )   2024.4   ISSN:13594184

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    In this article Noam Soreni at affiliation ‘Department of Psychiatry and Behavioural Neurosciences, McMaster University, Hamilton, Ontario, Canada and Offord Centre for Child Studies, Hamilton, Ontario, Canada’ was missing from the author list. The original article has been corrected.

    DOI: 10.1038/s41380-024-02494-9

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  • 小児神経と生命倫理学 生命倫理学と小児神経学のトランスレーション

    笹月 桃子, トカン・ヴラッド , 酒井 康成, 吉良 龍太郎, 大賀 正一

    脳と発達   56 ( 1 )   5 - 8   2024.1   ISSN:0029-0831

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    近年,神経・筋疾患,小児がん,造血器疾患に対する新しい遺伝学的解析法と治療薬が次々に開発され,これらは難治疾患を抱える子どもと家族に大きな希望を与えた.一方,小児科医が判断すべき治療法とその選択過程は複雑・多様化した.小児科医が子どもの代弁者として決断すべき選択肢は,法的・倫理的妥当性が許容される範囲内で,今後さらに多様化すると予測される.患児の利益をどのように擁護し,何を守るべきかに関して,主治医は決断までの過程を短縮・効率化することはできない.生と死の臨界点に際し,家族・医療者が双方に納得する合意を形成するには,合意を求めずに議論を尽くす覚悟もまた必要である.小児科におけるトランスレーショナル研究とは,実践臨床から生命倫理の本質を見出す努力に他ならず,答えのない難問について悩み続ける科学ではなかろうか.(著者抄録)

  • 【内部障害の理学療法における臨床思考の進め方のポイント】内部障害の理学療法における臨床思考の概念と理学療法の進め方のポイント

    山本 周平, 酒井 康成

    理学療法   40 ( 8 )   676 - 684   2023.8   ISSN:0910-0059

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    1.内部障害患者の理学療法の臨床思考の進め方に共通するポイントを理解し,臨床思考を進めることが求められる.2.内部障害の病態については原因と誘因に分けて整理する.3.入院中に機能低下(HAD)が生じ得ることを念頭に置き,定期的に機能評価を行うことを意識づける.4.チーム内カンファレンスの際には,アウトカムを共有する.5.理学療法介入の効果については目に見える効果と目に見えない効果に分けて考える.(著者抄録)

  • Seizure outcome of focus resection following evaluation using intracranial electrodes for pediatric epilepsy patients

    下川能史, 森岡隆人, 村上信哉, 橋口公章, 迎伸孝, 重藤寛史, 酒井康成, 酒田あゆみ, 酒田あゆみ, 渡邉恵利子, 吉本幸司

    小児の脳神経(Web)   48 ( 2 )   2023   ISSN:2435-824X

  • 神経免疫疾患のエビデンスに基づく診断基準・重症度分類・ガイドラインの妥当性と患者QOLの検証 第5回全国調査による日本人多発性硬化症・視神経脊髄炎の疫学および特徴

    磯部紀子, 渡邉充, 新野正明, 中島一郎, 松下拓也, 酒井康成, 中原仁, 河内泉, 河内泉, 越智博文, 中辻裕司, 福元尚子, 林史恵, 宮崎雄生, 藤盛寿一, 久冨木原健二, 久冨木原健二, 奥野龍禎, 中村優理, 中村優理, 中村優理, 迫田礼子, 迫田礼子, 米元耕輔, 平良遼志, 野村恭一, 山村隆, 藤原一男, 田中正美, 錫村明生, 清水優子, 清水潤, 園生雅弘, 松尾秀徳, 渡邊修, 深澤俊行, 荻野美恵子, 荻野美恵子, 郡山達男, 斎田孝彦, 野村芳子, 横山和正, 横山和正, 神田隆, 田原将行, 横田隆徳, 大橋高志, 鈴木則宏, 楠進, 栗山長門, 栗山長門, 和泉唯信, 小池春樹, 佐藤泰憲, 三澤園子, 村井弘之, 本村政勝, 吉川弘明, 中西恵美, 中村好一, 中村幸志, 坂田清美, 嶋田莉奈子, 松井真, 桑原聡, 吉良潤一, 吉良潤一, 吉良潤一

    神経免疫疾患のエビデンスに基づく診断基準・重症度分類・ガイドラインの妥当性と患者QOLの検証 令和4年度 総括・分担研究報告書(Web)   2023

  • Melatonin effect on neurodevelopment via IP3-mediated calcium regulation in autism spectrum disorder

    董双山, KIFUNE Takashi, KATO Hiroki, WANG Lu, KONG Jun, HIROFUJI Yuta, SATO Hiroshi, ITO Yosuke, KATO Takahiro A., SAKAI Yasunari, OHGA Shouichi, FUKUMOTO Satoshi, MASUDA Keiji

    日本生物学的精神医学会(Web)   45th   2023

  • 神経免疫疾患のエビデンスに基づく診断基準・重症度分類・ガイドラインの妥当性と患者QOLの検証 わが国の重症筋無力症の臨床像変化-全国疫学調査2006と2018の比較-

    吉川弘明, 中村好一, 栗山長門, 村井弘之, 酒井康成, 野村芳子, 松井真, 本村政勝, 鵜沢顕之, 今井富裕, 鈴木重明, 中根俊成, 足立由美, 岩佐和夫, 古川裕, 東昭孝

    神経免疫疾患のエビデンスに基づく診断基準・重症度分類・ガイドラインの妥当性と患者QOLの検証 令和4年度 総括・分担研究報告書(Web)   2023

  • 神経免疫疾患のエビデンスに基づく診断基準・重症度分類・ガイドラインの妥当性と患者QOLの検証 ランバート・イートン筋無力症候群-全国疫学調査2018より判明したわが国の課題-

    吉川弘明, 中村好一, 栗山長門, 村井弘之, 酒井康成, 野村芳子, 松井真, 本村政勝, 鵜沢顕之, 今井富裕, 鈴木重明, 中根俊成, 足立由美, 岩佐和夫, 古川裕, 東昭孝

    神経免疫疾患のエビデンスに基づく診断基準・重症度分類・ガイドラインの妥当性と患者QOLの検証 令和4年度 総括・分担研究報告書(Web)   2023

  • 小児科学 川崎病の病態と酸化リン脂質

    中島 康貴, 酒井 康成, 原 寿郎

    医学のあゆみ   283 ( 3 )   217 - 218   2022.10   ISSN:0039-2359

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  • 【頭痛診療のUp to Date】小児の頭痛

    園田 有里, 酒井 康成, 大賀 正一

    臨牀と研究   99 ( 7 )   883 - 886   2022.7   ISSN:0021-4965

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  • 高度徐脈に対して緊急ペースメーカー埋込術を施行した抗NMDA受容体脳炎の一例

    赤峰 哲, 松原 祥恵, 川上 沙織, 鳥尾 倫子, チョン・ピン・フィー, 酒井 康成, 吉良 龍太郎

    脳と発達   54 ( Suppl. )   S352 - S352   2022.5   ISSN:0029-0831 eISSN:1884-7668

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  • 神経変性ランゲルハンス細胞組織球症に対する大量免疫グロブリン療法の有効性

    園田 有里, 藤井 史彦, 園田 素史, 石村 匡崇, 一宮 優子, チョン・ピン・フィー, 米元 耕輔, 平良 遼志, 實藤 雅文, 古賀 友紀, 酒井 康成, 大賀 正一

    脳と発達   54 ( Suppl. )   S214 - S214   2022.5   ISSN:0029-0831 eISSN:1884-7668

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  • 抗Sm抗体陽性の可逆性脳血管攣縮症候群の一例

    一宮 優子, 鳥尾 倫子, 松田 あかね, 平良 遼志, 米元 耕輔, 園田 有里, チョン・ピン・フィー, 實藤 雅文, 酒井 康成, 大賀 正一

    脳と発達   54 ( Suppl. )   S257 - S257   2022.5   ISSN:0029-0831 eISSN:1884-7668

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  • 乳児型ポンペ病における新生児マススクリーニングと早期治療の有用性

    松田 あかね, トカンヴラッド, 平田 悠一郎, 長友 雄作, チョン・ピン・フィー, 園田 有里, 一宮 優子, 永田 弾, 虫本 雄一, 石井 加奈子, 山村 健一郎, 實藤 雅文, 酒井 康成, 中村 公俊, 大賀 正一

    脳と発達   54 ( Suppl. )   S245 - S245   2022.5   ISSN:0029-0831 eISSN:1884-7668

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  • ヒト誘導ミクログリアを用いた亜急性硬化性全脳炎におけるIL-17シグナル解析

    藤井 史彦, 米元 耕輔, 平良 遼志, 扇谷 昌宏, 加藤 隆弘, 酒井 康成, 大賀 正一

    脳と発達   54 ( Suppl. )   S213 - S213   2022.5   ISSN:0029-0831 eISSN:1884-7668

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  • ヒト誘導ミクログリアにおける細胞不均一性の解析

    米元 耕輔, 藤井 史彦, 平良 遼志, チョン・ピン・フィー, 扇谷 昌宏, 加藤 隆弘, 酒井 康成, 大賀 正一

    脳と発達   54 ( Suppl. )   S268 - S268   2022.5   ISSN:0029-0831 eISSN:1884-7668

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  • 【免疫性神経疾患(第2版)-基礎・臨床の最新知見-】中枢神経脱髄疾患 急性散在性脳脊髄炎(ADEM)

    藤井 史彦, チョン・ピン・フィー , 酒井 康成

    日本臨床   80 ( 増刊5 免疫性神経疾患 )   225 - 228   2022.5   ISSN:0047-1852

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  • 【免疫性神経疾患(第2版)-基礎・臨床の最新知見-】中枢神経脱髄疾患 急性散在性脳脊髄炎(ADEM)

    藤井 史彦, チョン・ピン・フィー, 酒井 康成

    日本臨床   80 ( 増刊5 免疫性神経疾患 )   225 - 228   2022.5   ISSN:0047-1852

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  • Gαoはニューロン成長円錐の成熟を促す

    平良 遼志, 藤井 史彦, 米元 耕輔, 赤峰 哲, 酒井 康成, 大賀 正一

    脳と発達   54 ( Suppl. )   S270 - S270   2022.5   ISSN:0029-0831 eISSN:1884-7668

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  • 小児抗MOG抗体関連疾患におけるリピドミクス解析

    チョン・ピン・フィー, 藤井 史彦, 平良 遼志, 米元 耕輔, 園田 有里, 一宮 優子, 實藤 雅文, 酒井 康成, 吉良 龍太郎, 大賀 正一

    脳と発達   54 ( Suppl. )   S267 - S267   2022.5   ISSN:0029-0831 eISSN:1884-7668

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  • 新生児マススクリーニングで発見され早期治療介入が可能であった乳児型ポンペ病の1例

    松田 あかね, 石井 加奈子, 虫本 雄一, 戸田 尚子, トカンヴラッド, 平田 悠一郎, 實藤 雅文, 酒井 康成, 大賀 正一, 澤田 貴彰, 中村 公俊

    日本小児科学会雑誌   126 ( 3 )   571 - 571   2022.3   ISSN:0001-6543

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    J-GLOBAL

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  • 喉頭けいれんと肝脾腫を呈し、薬理学的シャペロン療法が有効であったGaucher病2型の1女児例

    東 加奈子, 賀来 典之, 松岡 若利, 福岡 将治, 鉄原 健一, トカンヴラッド, 虫本 雄一, 藤井 史彦, 園田 有里, 一宮 優子, 酒井 康成, 大賀 正一, 中村 公俊

    日本小児科学会雑誌   126 ( 3 )   571 - 571   2022.3   ISSN:0001-6543

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  • 喉頭けいれん、肝脾腫から診断に至り、ambroxol大量療法が有効であったGaucher病2型の1歳女児例

    中尾 泰介, 藤井 史彦, 園田 有里, 東 加奈子, 虫本 雄一, 賀来 典之, 鳥尾 倫子, 一宮 優子, チョン・ピンフィー, 實藤 雅文, 酒井 康成, 中村 公俊, 大賀 正一

    脳と発達   54 ( 1 )   66 - 66   2022.1   ISSN:0029-0831 eISSN:1884-7668

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  • 神経免疫疾患の診断・重症度分類・ガイドラインの妥当性検証・改定と全国調査による患者QOLの改善の検証 腫瘍合併の有無によるランバート・イートン筋無力症候群の臨床像

    吉川弘明, 中村好一, 栗山長門, 村井裕之, 酒井康成, 野村芳子, 足立由美, 岩佐和夫, 古川裕, 東昭孝, 松井真, 桑原聡

    神経免疫疾患のエビデンスに基づく診断基準・重症度分類・ガイドラインの妥当性と患者QOLの検証 令和3年度 総括・分担研究報告書(Web)   2022

  • ランバート・イートン筋無力症候群の臨床像-腫瘍の有無による比較-

    吉川弘明, 中村好一, 栗山長門, 村井弘之, 酒井康成, 野村芳子, 足立由美, 岩佐和夫, 古川裕, 東昭孝, 松井真, 桑原聡

    日本神経学会学術大会プログラム・抄録集   63rd   2022

  • ”de novo psychosis” following epilepsy surgery: three case reports

    三苫良, 下川能史, 迎伸孝, 酒井康成, 重藤寛史, 重藤寛史, 酒田あゆみ, 酒田あゆみ, 渡邊恵利子, 平野昭吾, 平野羊嗣

    てんかん研究   40 ( 2 )   2022   ISSN:0912-0890

  • 神経免疫疾患の診断・重症度分類・ガイドラインの妥当性検証・改定と全国調査による患者QOLの改善の検証 第5回全国調査からみた多発性硬化症の重症度に寄与する因子の検討

    磯部紀子, 渡邉充, 新野正明, 中島一郎, 松下拓也, 酒井康成, 中原仁, 河内泉, 河内泉, 越智博文, 中辻裕司, 福元尚子, 林史恵, 宮崎雄生, 藤盛寿一, 久冨木原健二, 奥野龍禎, 中村優理, 中村優理, 迫田礼子, 米元耕輔, 平良遼志, 野村恭一, 山村隆, 藤原一男, 田中正美, 錫村明生, 清水優子, 清水潤, 園生雅弘, 松尾秀徳, 渡邊修, 深澤俊行, 荻野美恵子, 郡山達男, 斎田孝彦, 野村芳子, 横山和正, 神田隆, 田原将行, 横田隆徳, 大橋高志, 鈴木則宏, 楠進, 栗山長門, 栗山長門, 和泉唯信, 小池春樹, 佐藤泰憲, 三澤園子, 村井弘之, 本村政勝, 吉川弘明, 中西恵美, 中村好一, 中村幸志, 坂田清美, 嶋田莉奈子, 松井真, 桑原聡, 吉良潤一, 吉良潤一, 吉良潤一

    神経免疫疾患のエビデンスに基づく診断基準・重症度分類・ガイドラインの妥当性と患者QOLの検証 令和3年度 総括・分担研究報告書(Web)   2022

  • ランバート・イートン筋無力症候群の全国疫学調査(2018)

    吉川 弘明, 中村 好一, 栗山 長門, 村井 弘之, 酒井 康成, 野村 芳子, 足立 由美, 岩佐 和夫, 古川 裕, 東 昭孝, 松井 真

    臨床神経学   2021.9

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  • 18トリソミー児の無呼吸に対する管理と予後

    井上 普介, 江上 直樹, 安岡 和昭, 澤野 徹, 市山 正子, 藤吉 順子, 落合 正行, 平良 遼志, 酒井 康成, 大賀 正一

    日本周産期・新生児医学会雑誌   2019.6

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  • 【小児疾患の診断治療基準】(第2部)疾患 神経・筋疾患 多発性硬化症

    高田 結, 酒井 康成

    小児内科   2018.11

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  • 発症時に表情変化を伴わない笑い発作を呈したPallister-Hall症候群の1例

    米元 耕輔, 鳥尾 倫子, 酒井 康成, 酒田 あゆみ, 大賀 正一

    脳と発達   2018.9

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    Pallister-Hall症候群(PHS)は、多指症、視床下部過誤腫を含む多発奇形と精神運動発達遅滞を特徴とする遺伝性疾患である。視床下部過誤腫に伴う笑い発作がてんかん発作の特徴として知られるが、初発時の診断に苦慮する場合がある。症例は3歳女児。新生児期に多指症、直腸腟前庭瘻および視床下部過誤腫が認められPHSと診断された。生後2ヵ月から、表情変化のない、咳き込み様の症状が出現し、10ヵ月および2歳2ヵ月時に同症状の頻度が増加したが、脳波上異常所見なし。3歳時、同様の咳き込み症状は笑い表情を伴うようになり、症状に一致して全般性徐波が認められた。Valproateとclobazamを併用し、笑い発作は良好にコントロールされた。乳児期早期の笑い発作は、必ずしも表情変化を伴わない場合がある。笑い発作は本症例のように無治療で増減を繰り返すことがあり、症状減少後も長期的フォローアップが必要である。(著者抄録)

  • 知的障害、小脳失調、外眼筋麻痺および深部腱反射消失を認めたinfantile-onset spinocerebellar ataxiaの1例

    鳥尾 倫子, 平良 遼志, 園田 有里, 赤峰 哲, 石崎 義人, 實藤 雅文, 酒井 康成, 大賀 正一

    脳と発達   2018.5

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  • 一過性メチルマロン酸血症を呈したCDKL5脳症の男児例(A male case with CDKL5-associated encephalopathy manifesting transient methylmalonic acidemia)

    赤峰 哲, 石崎 義人, 酒井 康成, 鳥巣 浩幸, 深井 綾子, 三宅 紀子, 大久保 和宏, 實藤 雅文, 酒田 あゆみ, 木村 正彦, 山口 清次, 坂本 修, 才津 浩智, 松本 直通, 大賀 正一

    脳と発達   2018.5

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  • 小児発症抗MOG抗体関連疾患患者の実態把握のための全国調査研究

    東川 幸嗣, 中島 一郎, 金子 公彦, 鳥巣 浩幸, 酒井 康成, 吉良 龍太郎, 佐久間 啓, 田中 惠子, 中嶋 秀人, 島川 修一, 玉井 浩

    脳と発達   2018.5

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    小児発症抗MOG抗体関連疾患患者の実態把握のための全国調査研究

  • 新生児臨床研究ネットワークデータベースに基づいた出生体重500g以下児の3歳時神経学的予後の調査

    井上 普介, 松下 悠紀, 落合 正行, 石崎 義人, 實藤 雅文, 酒井 康成, 大賀 正一

    脳と発達   2018.5

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  • 極低出生体重児に合併するてんかんの特徴

    鳥尾 倫子, 落合 正行, 園田 有里, 石崎 義人, 實藤 雅文, 酒井 康成, 大賀 正一

    脳と発達   2018.5

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  • 白血病の治療中に腸管定着型ボツリヌス症を発症した5歳男児

    大山 紀子, 鳥尾 倫子, 中島 健太郎, 古賀 友紀, 西尾 壽乗, 神野 俊介, 酒井 康成, 加藤 はる, 朝倉 宏, 大賀 正一

    日本小児科学会雑誌   2018.2

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  • NRNデータベースから示される極低出生体重児の気管切開の危険因子

    倉田 浩昭, 落合 正行, 井上 普介, 市山 正子, 安岡 和昭, 藤吉 順子, 松下 悠紀, 酒井 康成, 大賀 正一

    日本小児科学会雑誌   2018.2

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  • 先天性門脈体循環シャントに合併した肺高血圧に対する閉鎖治療の効果

    鵜池 清, 永田 弾, 藤井 俊輔, 松岡 良平, 江口 祥美, 村岡 衛, 福岡 将治, 長友 雄作, 大久保 一宏, 平田 悠一郎, 石井 加奈子, 酒井 康成, 大賀 正一, 松浦 俊治, 田口 智章

    日本小児肺循環研究会プログラム・抄録集   2018.2

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  • 一般小児科外来における小児二次性頭痛の検討

    園田 有里, 野田 麻里絵, 兒玉 志保, 武本 環美, 酒井 康成, 大賀 正一

    日本頭痛学会誌   2017.11

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  • 白血病治療中に発症した腸管定着型ボツリヌス症

    大山 紀子, 鳥尾 倫子, 古賀 友紀, 西尾 寿乗, 神野 俊介, 中島 健太郎, 實藤 雅文, 石崎 義人, 酒井 康成, 大賀 正一, 朝倉 宏, 加藤 はる

    日本小児科学会雑誌   2017.10

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  • 白血病の治療中に腸管定着型ボツリヌス症を発症した5歳男児

    大山 紀子, 鳥尾 倫子, 中島 健太郎, 古賀 友紀, 西尾 壽乗, 神野 俊介, 酒井 康成, 加藤 はる, 朝倉 宏, 大賀 正一

    NEUROINFECTION   2017.9

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  • 咳嗽との鑑別を要し、自然軽快と再発を示した笑い発作の一例

    鳥尾 倫子, 酒井 康成, 米元 耕輔, 石崎 義人, 實藤 雅文, 大賀 正一, 橋口 公章

    てんかん研究   2017.9

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  • Kawasaki disease: a matter of innate immunity

    Hara T, Nakashima Y, Sakai Y, Nishio H, Motomura Y, Yamasaki S

    Clin Exp Immunol (in press)   2017.6

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  • 腰椎腹腔シャント術を要した特発性頭蓋内圧亢進症の一例

    米元 耕輔, 鳥尾 倫子, 酒井 康成, 空閑 大亮, 笹月 桃子, 石崎 義人, 實藤 雅文, 鳥巣 浩幸, 高田 英俊, 大賀 正一

    脳と発達   2017.5

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  • 小児後天性脱髄症候群の多発性硬化症発症に関連する因子の検討

    高田 結, 鳥巣 浩幸, 酒井 康成, 赤峰 哲, 鳥尾 倫子, 石崎 義人, 實藤 雅文, 笹月 桃子, 原 寿郎, 高田 英俊, 大賀 正一

    脳と発達   2017.5

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  • 新生児臨床研究ネットワークデータベースに基づいた極低出生体重児の神経学的後遺症に関わる予後因子の解析

    松下 悠紀, 落合 正行, 井上 普介, 米元 耕輔, 赤峰 哲, 石崎 義人, 實藤 雅文, 酒井 康成, 高田 英俊, 大賀 正一

    脳と発達   2017.5

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  • 白血病治療中の5歳児に発症した成人腸管定着型ボツリヌス症

    大山 紀子, 鳥尾 倫子, 加藤 はる, 酒井 康成, 實藤 雅文, 石崎 義人, 古賀 友紀, 鳥巣 浩幸, 大賀 正一

    脳と発達   2017.5

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  • 笑い発作の自然軽快と再発を示したPallister-Hall症候群の一例

    鳥尾 倫子, 酒井 康成, 米元 耕輔, 石崎 義人, 實藤 雅文, 笹月 桃子, 鳥巣 浩幸, 高田 英俊, 大賀 正一

    脳と発達   2017.5

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  • CHD2デノボ変異を認めたLennox-Gastaut症候群の13歳女児

    赤峰 哲, 酒井 康成, 笹月 桃子, 鳥巣 浩幸, 實藤 雅文, 石崎 義人, 高田 英俊, 才津 浩智, 中島 光子, 松本 直通, 大賀 正一

    てんかん研究   2017.1

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  • 早産極低出生体重児における3歳時てんかん発症率と周産期危険因子

    松下 悠紀, 安岡 和昭, 田中 幸一, 菅 秀太郎, 倉田 浩昭, 井上 普介, 藤吉 順子, 落合 正行, 大賀 正一, 酒井 康成

    日本新生児成育医学会雑誌   2016.11

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  • Kawasaki disease: a matter of innate immunity

    T. Hara, Y. Nakashima, Y. Sakai, H. Nishio, Y. Motomura, S. Yamasaki

    Clinical and Experimental Immunology   2016.11

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    Kawasaki disease (KD) is an acute systemic vasculitis of childhood that does not have a known cause or aetiology. The epidemiological features (existence of epidemics, community outbreaks and seasonality), unique age distribution and clinical symptoms and signs of KD suggest that the disease is caused by one or more infectious environmental triggers. However, KD is not transmitted person-to-person and does not occur in clusters within households, schools or nurseries. KD is a self-limited illness that is not associated with the production of autoantibodies or the deposition of immune complexes, and it rarely recurs. Regarding the underlying pathophysiology of KD, innate immune activity (the inflammasome) is believed to play a role in the development of KD vasculitis, based on the results of studies with animal models and the clinical and laboratory findings of KD patients. Animal studies have demonstrated that innate immune pathogen-associated molecular patterns (PAMPs) can cause vasculitis independently of acquired immunity and have provided valuable insights regarding the underlying mechanisms of this phenomenon. To validate this concept, we recently searched for KD-specific PAMPs and identified such molecules with high specificity and sensitivity. These molecules have structures similar to those of microbe-associated molecular patterns (MAMPs), as shown by liquid chromatography-tandem mass spectrometry. We propose herein that KD is an innate immune disorder resulting from the exposure of a genetically predisposed individual to microbe-derived innate immune stimulants and that it is not a typical infectious disease.

    DOI: 10.1111/cei.12832

  • 小児神経科医が知っておくべき遺伝学的検査シリーズ 先天性無虹彩症、成長不良および発達の遅れを示す10ヵ月女児

    酒井 康成

    脳と発達   2016.11

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  • 小児期発症の歯状核赤核淡蒼球ルイ体萎縮症4例のてんかんの臨床像

    鳥尾 倫子, 酒井 康成, 實藤 雅文, 石崎 義人, 高田 英俊

    てんかん研究   2016.9

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  • CHD2デノボ変異を認めたLennox-Gastaut症候群の13歳女児

    赤峰 哲, 酒井 康成, 笹月 桃子, 鳥巣 浩幸, 高田 英俊, 大賀 正一, 才津 浩智, 中島 光子, 松本 直通

    てんかん研究   2016.9

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  • 驚愕反応、てんかん、不随意運動を呈しKCNH1新規デノボ変異を認めたTemple-Baraitser症候群の1例

    赤峰 哲, 鳥尾 倫子, 酒井 康成

    脳と発達   2016.5

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  • けいれん発作を伴わず不随意運動が主体であったDRPLAの1例

    鳥尾 倫子, 酒井 康成, 實藤 雅文, 石崎 義人, 鳥巣 浩幸, 高田 英俊

    脳と発達   2016.5

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  • 重症心身障害児の諸症状に対する漢方治療による介入の経験

    宮田 潤子, 貝沼 茂三郎, 江角 元史郎, 鳥尾 倫子, 酒井 康成, 永田 公二, 松浦 俊治, 木下 義晶, 田口 智章

    日本小児外科学会雑誌   2016.2

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  • もやもや病遺伝子RNF213は炎症性シグナルと血管新生をつなぐ分子である

    大久保 一宏, 酒井 康成, 赤峰 哲, 石崎 義人, 松下 悠紀, 實藤 雅文, 鳥巣 浩幸, 井原 健二, 高田 英俊, 原 寿郎

    日本小児科学会雑誌   2016.2

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  • 早産児infantile spasmsにおけるZonisamideの有効性

    鳥尾 倫子, 實藤 雅文, 酒井 康成, 石崎 義人, 鳥巣 浩幸, 笹月 桃子, 高田 英俊

    日本小児科学会雑誌   2016.2

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  • 遅発性拡散能低下を呈したshaken baby syndromeの10ヵ月女児

    赤峰 哲, 李 守永, 酒井 康成

    脳と発達   2016.1

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  • ローランド発射が先行した若年性ミオクロニーてんかんの1例

    石崎 義人, 酒井 康成, 實藤 雅文, 鳥尾 倫子, 赤峰 哲, 高田 英俊

    てんかん研究   2016.1

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  • 早産児infantile spasmsにおける治療反応性の特徴

    鳥尾 倫子, 實藤 雅文, 酒井 康成, 石崎 義人, 鳥巣 浩幸, 笹月 桃子, 高田 英俊

    てんかん研究   2015.9

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  • 軟骨無形成症の児のチャイルドシート使用2例に関するinjury alert

    二宮 崇仁, 金政 光, 平田 悠一郎, 松岡 若利, 李 守永, 賀来 典之, 中島 健太郎, 鳥尾 倫子, 酒井 康成, 石崎 義人, 實藤 雅文, 落合 正行, 原 寿郎, 橋口 公章

    日本小児科学会雑誌   2015.8

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  • 発熱に伴い反復性の脱力と持続的な不随意運動を示した小児交互性片麻痺の1例

    鳥尾 倫子, 酒井 康成, 實藤 雅文, 李 守永, 石崎 義人, 鳥巣 浩幸, 才津 浩智, 松本 直通, 原 寿郎

    脳と発達   2015.7

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  • 難治性てんかんおよび重度精神遅滞を示す女性に見いだされたNF1およびMAGEL2ダブル変異と遺伝的相乗効果

    赤峰 哲, 酒井 康成, 鳥尾 倫子, 石崎 義人, 實藤 雅文, 鳥巣 浩幸, 才津 浩智, 松本 直通, 原 寿郎

    脳と発達   2015.5

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  • 乳児期発症のてんかん児に見いだされたde novo TRIM8変異とその遺伝的修飾因子

    酒井 康成, 赤峰 哲, 實藤 雅文, 鳥尾 倫子, 石崎 義人, 才津 浩智, 鳥巣 浩幸, 松本 直通, 原 寿郎

    脳と発達   2015.5

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  • 当院におけるウエスト症候群50例の検討

    鳥尾 倫子, 酒井 康成, 實藤 雅文, 笹月 桃子, 石崎 義人, 鳥巣 浩幸, 原 寿郎

    脳と発達   2015.5

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  • 熱性けいれん重積と急性脳症の鑑別における経時的脳波周波数解析の有効性の検討

    李 守永, 酒井 康成, 實藤 雅文, 石崎 義人, 鳥尾 倫子, 鳥巣 浩幸, 原 寿郎

    脳と発達   2015.5

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  • 自然免疫受容体Nod1のリガンドは母仔の血管病変を介してIUGRおよびIUFDをおこす

    井上 普介, 西尾 寿乗, 高田 英俊, 酒井 康成, 名西 悦郎, 落合 正行, 原 寿郎

    日本産婦人科・新生児血液学会誌   2015.5

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  • 日齢7に発症した乳児悪性焦点移動性部分てんかんの女児例

    赤峰 哲, 酒井 康成, 鳥尾 倫子, 李 守永, 石崎 義人, 實藤 雅文, 才津 浩智, 松本 直通, 原 寿郎

    脳と発達   2015.3

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  • デノボNALCN変異を有し、特徴的顔貌、精神運動発達遅滞および高カルシウム血症を示した4歳女児

    鳥尾 倫子, 酒井 康成, 松下 悠紀, 戸田 尚子, 井原 健二, 石崎 義人, 笹月 桃子, 原 寿郎

    脳と発達   2015.3

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  • てんかん発症2年後に出血性ショック脳症症候群を来した超低出生体重児の一例

    赤峰 哲, 加藤 稚子, 李 守永, 酒井 康成, 原 寿郎

    てんかん研究   2015.1

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  • 幼児期より両側頭頂側頭部に棘波を認めた、PRRT2変異を伴う部分てんかんの一例

    鳥巣 浩幸, 渡辺 恭子, 下島 圭子, 島田 姿野, 實藤 雅文, 石崎 義人, 酒井 康成, 山本 俊至, 奥村 彰久, 原 寿郎

    てんかん研究   2014.9

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  • 意識障害と広範な大脳白質病変を呈した家族性血球貪色症候群III型の15歳女児例

    赤峰 哲, 黒川 麻里, 大場 詩子, 石村 匡崇, 瀧本 智仁, 石崎 義人, 古賀 友紀, 酒井 康成, 鳥巣 浩幸, 住江 愛子, 高田 英俊, 大賀 正一, 原 寿郎

    脳と発達   2014.9

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  • 自験例2例を含む近年の小児期発症日本脳炎の報告

    名西 悦郎, 保科 隆之, 西尾 壽乘, 門屋 亮, 酒井 康成, 大賀 正一, 原 寿郎

    感染症学雑誌   2014.9

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  • 経過中に広範な白質病変が出現したミトコンドリアDNA枯渇症候群の女児例

    園田 有里, 石崎 義人, 西尾 壽乘, 李 守永, 鳥尾 倫子, 礒部 菜摘, 實藤 雅文, 酒井 康成, 鳥巣 浩幸, 原 寿郎

    脳と発達   2014.7

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  • 精神運動発達遅滞、てんかんの経過中にふらつきが出現した歯状核赤核淡蒼球ルイ体萎縮症の女児例

    礒部 菜摘, 酒井 康成, 實藤 雅文, 石崎 義人, 鳥巣 浩幸, 原 寿郎

    脳と発達   2014.7

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  • 神経芽腫に対する化学療法中に辺縁系脳炎をきたした1例

    中島 健太郎, 加藤 稚子, 大場 詩子, 小野 宏彰, 古賀 友紀, 住江 愛子, 酒井 康成, 高田 英俊, 原 寿郎, 宗崎 良太, 木下 義晶, 田口 智章, 三好 きな, 孝橋 賢一, 小田 義直

    日本小児血液・がん学会雑誌   2014.6

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  • 自験例2例を含む近年の小児期発症日本脳炎の報告

    名西 悦郎, 保科 隆之, 西尾 壽乘, 門屋 亮, 酒井 康成

    日本化学療法学会雑誌   2014.5

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  • けいれん重積型脳症9例に対する治療および転帰に関する後方視的検討

    李 守永, 鳥巣 浩幸, 石崎 義人, 實藤 雅文, 鳥尾 倫子, 酒井 康成, 原 寿郎

    脳と発達   2014.5

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  • PTEN遺伝子変異にともなう脳内トランスクリプトーム変化と治療標的の探索

    酒井 康成

    ライフサイエンス振興財団年報   2014.3

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  • 自閉症の神経科学的研究 自閉症発症に関与する分子シグナルの探索

    酒井 康成

    脳と発達   2013.5

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  • PRRT2変異を有するICCA症候群家系に認めた、幼児期発症部分てんかんの一女児例

    鳥巣 浩幸, 渡辺 恭子, 下島 圭子, 島田 姿野, 實藤 雅文, 石崎 義人, 酒井 康成, 山本 俊至, 奥村 彰久, 原 寿郎

    脳と発達   2013.5

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  • けいれん重積後より持続脳波モニタリングを行ったけいれん重積型脳症患者の脳波周波数解析

    李 守永, 鳥巣 浩幸, 石崎 義人, 實藤 雅文, 酒井 康成, 原 寿郎

    脳と発達   2013.5

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  • 亜急性硬化性全脳炎と麻疹ワクチン応答の個人差に関連する遺伝子多型についての検討

    石崎 義人, 鳥巣 浩幸, 酒井 康成, 李 守永, 實藤 雅文, 原 寿郎

    脳と発達   2013.5

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  • 日本脳炎経過中のMRI、SPECT、脳波所見 10歳男児例

    礒部 菜摘, 鳥巣 浩幸, 實藤 雅文, 李 守永, 石崎 義人, 酒井 康成, 原 寿郎

    脳と発達   2013.5

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  • 熱性けいれん重積とけいれん重積型脳症の鑑別における急性期脳波周波数解析

    李 守永, 鳥巣 浩幸, 石崎 義人, 實藤 雅文, 酒井 康成, 原 寿郎

    脳と発達   2013.5

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  • 特徴的顔貌、成長障害、精神運動発達遅滞、高カルシウム血症を呈した遠位関節拘縮症の女児例

    鳥尾 倫子, 鳥巣 浩幸, 酒井 康成, 礒部 菜摘, 石崎 義人, 實藤 雅文, 原 寿郎

    脳と発達   2013.5

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  • 脳血管周囲腔の拡張を認めたLoeys-Dietz症候群の男児例

    鵜池 清, 松下 悠紀, 酒井 康成, 園田 有里, 石崎 義人, 実藤 雅文, 永田 弾, 山村 健一郎, 鳥巣 浩幸, 原 寿郎

    日本小児科学会雑誌   2013.2

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  • 熱性けいれん後の意識障害と急性脳症の鑑別における急性期脳波の有効性

    李 守永, 鳥巣 浩幸, 實藤 雅文, 石崎 義人, 酒井 康成, 原 寿郎

    てんかん研究   2013.1

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  • 診断が困難であった日本脳炎の小児例

    深澤 光晴, 實藤 雅文, 西山 慶, 李 守永, 鳥巣 浩幸, 石崎 義人, 酒井 康成, 原 寿郎

    NEUROINFECTION   2012.10

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  • アデノウイルス関連急性脳炎・心筋炎の6歳男児例

    李 守永, 鳥巣 浩幸, 賀来 典之, 馬場 晴久, 石崎 義人, 實藤 雅文, 酒井 康成, 原 寿郎

    NEUROINFECTION   2012.10

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  • 有熱時けいれん・意識障害を呈する小児における急性脳症の判別に関する検討

    鳥巣 浩幸, 李 守永, 賀来 典之, 實藤 雅文, 石崎 義人, 酒井 康成, 馬場 晴久, 原 寿郎

    NEUROINFECTION   2012.10

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  • 小児急性脳症と熱性けいれんの鑑別における初期脳波の有効性の検討

    李 守永, 鳥巣 浩幸, 實藤 雅文, 石崎 義人, 山口 結, 酒井 康成, 板倉 朋子, 酒田 あゆみ, 原 寿郎

    てんかん研究   2012.9

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  • West症候群を発症したMCAP/MPPH症候群の乳児例

    鳥巣 浩幸, 金城 唯宗, 實藤 雅文, 石崎 義人, 酒井 康成, 村上 信哉, 萩原 綱一, 板倉 朋子, 酒田 あゆみ, 原 寿郎

    てんかん研究   2012.9

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  • 日本脳炎の10歳男児例

    深澤 光晴, 實藤 雅文, 西山 慶, 李 守永, 石崎 義人, 鳥巣 浩幸, 酒井 康成, 原 寿郎

    日本小児科学会雑誌   2012.8

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  • 熱性けいれん重積と急性脳症の鑑別における急性期脳波の有効性の検討

    李 守永, 鳥巣 浩幸, 山口 結, 石崎 義人, 實藤 雅文, 酒井 康成, 原 寿郎

    脳と発達   2012.5

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  • Leigh脳症の経過中に神経原性肺水腫を呈した男児例

    松尾 光通, 石崎 義人, 李 守永, 鳥尾 倫子, 山口 結, 實藤 雅文, 酒井 康成, 鳥巣 浩幸, 原 寿郎

    脳と発達   2012.5

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  • MEF2C遺伝子を含む染色体5q14.3領域の欠失を認めた重度精神遅滞の14歳男児例

    酒井 康成, 鳥巣 浩幸, 實藤 雅史, 山口 結, 石崎 義人, 鳥尾 倫子, 磯部 菜採, 佐竹 宏之, 原 寿郎

    脳と発達   2012.5

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  • もやもや病感受性遺伝子RNF213の検討

    石崎 義人, 鳥巣 浩幸, 酒井 康成, 實藤 雅文, 山口 結, 原 寿郎

    脳と発達   2012.5

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  • 反復性過眠症の臨床的検討 インフルエンザと過眠症の関連

    鳥尾 倫子, 鳥巣 浩幸, スビヤント・ケイジ, 實藤 雅文, 石崎 義人, 酒井 康成, 神林 崇, 權藤 健二郎, 原 寿郎

    脳と発達   2012.5

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  • 急性中枢神経症状を呈する発熱小児における急性脳症の判別

    鳥巣 浩幸, 李 守永, 山口 結, 石崎 義人, 實藤 雅文, 酒井 康成, 原 寿郎

    脳と発達   2012.5

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  • 近赤外分光法による、無意味図形の想起の成功の研究 光路長の影響を受けにくい解析法

    實藤 雅文, 吉良 龍太郎, 岩山 真理子, 酒井 康成, 鳥巣 浩幸, 原 寿郎

    日本小児科学会雑誌   2007.4

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    近赤外分光法による、無意味図形の想起の成功の研究 光路長の影響を受けにくい解析法

  • NIRSによる、無意味図形の想起の成功の研究 光路長の影響を受けにくい解析法

    實藤 雅文, 吉良 龍太郎, 鳥巣 浩幸, 酒井 康成, 原 寿郎

    日本小児科学会雑誌   2007.2

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    NIRSによる、無意味図形の想起の成功の研究 光路長の影響を受けにくい解析法

  • 軽症下痢に伴うけいれんを認めた良性家族性乳児けいれんの姉弟例

    吉良 龍太郎, 酒井 康成, 鳥巣 浩幸, 齋藤 光正, 楠原 浩一, 原 寿郎, 安元 佐和

    日本小児科学会雑誌   2006.9

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    軽症下痢に伴うけいれんを認めた良性家族性乳児けいれんの姉弟例

  • 脳機能と遺伝子多型の関連の研究 2.言語と転写因子(GTF2IRD1)

    實藤 雅文, 武本 環美, 濱本 香織, 中島 大輔, 吉良 龍太郎, 鳥巣 浩幸, 酒井 康成, 岩山 真理子, 原 寿郎

    脳と発達   2006.5

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    脳機能と遺伝子多型の関連の研究 2.言語と転写因子(GTF2IRD1)

  • 亜急性硬化性全脳炎におけるdsRNA認識関連分子の遺伝子多型解析

    武本 環美, 楠原 浩一, 吉良 龍太郎, 鳥巣 浩幸, 石崎 義人, 酒井 康成, 原 寿郎

    脳と発達   2006.5

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    亜急性硬化性全脳炎におけるdsRNA認識関連分子の遺伝子多型解析

  • 新規NPC1遺伝子変異を認めた乳児期発症Niemann-Pick病C型の日本人同胞例

    鳥巣 浩幸, 吉良 龍太郎, 高橋 勉, 由茅 直子, 酒井 康成, 武本 環美, 実藤 雅史, 石崎 義人, 原 寿郎

    脳と発達   2006.5

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    新規NPC1遺伝子変異を認めた乳児期発症Niemann-Pick病C型の日本人同胞例

  • 気体プラズマを用いた新たな遺伝子導入法

    酒井 康成, Vahid Khajoee, 小川 恭弘, 片山 佳樹, 楠原 浩一, 原 寿郎

    日本小児科学会雑誌   2006.2

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  • 親の年齢と子どもの異常の関係 福岡市乳幼児健康診断のデータから

    岩山 真理子, 吉良 龍太郎, 酒井 康成, 鳥巣 浩幸, 原 寿郎, 絹川 直子, 山田 知美, 野瀬 善明

    日本小児科学会雑誌   2005.12

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    親の年齢と子どもの異常の関係 福岡市乳幼児健康診断のデータから

  • SSPEにおける末梢血単核球の網羅的遺伝子発現解析

    武本 環美, 吉良 龍太郎, 楠原 浩一, 鳥巣 浩幸, 酒井 康成, 原 寿郎

    NEUROINFECTION   2005.9

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    SSPEにおける末梢血単核球の網羅的遺伝子発現解析

  • 進行性の左片麻痺をきたした9歳男児例

    原 卓也, 酒井 康成, 鳥巣 浩幸, 吉良 龍太郎, 原 寿郎

    脳と発達   2005.7

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    進行性の左片麻痺をきたした9歳男児例

  • PLP1遺伝子の完全欠失を認めるPelizaeus-Merzbacher病(PLP null症候群)の1男児例

    鳥巣 浩幸, 吉良 龍太郎, 酒井 康成, 實藤 雅文, 原 寿郎, 岩城 明子, 竹下 研三

    脳と発達   2005.7

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    PLP1遺伝子の完全欠失を認めるPelizaeus-Merzbacher病(PLP null症候群)の1男児例

  • 脳機能と遺伝子多型の関連の研究 1.記憶と代謝型グルタミン受容体遺伝子多型

    實藤 雅文, 濱本 香織, 吉良 龍太郎, 柴田 弘紀, 中島 大輔, 鳥巣 浩幸, 酒井 康成, 武本 環美, 岩山 真理子, 服巻 保幸, 原 寿郎

    脳と発達   2005.5

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    脳機能と遺伝子多型の関連の研究 1.記憶と代謝型グルタミン受容体遺伝子多型

  • PLP1遺伝子完全欠失によるPelizaeus-Merzbacher病(PLP null症候群)の男児例

    鳥巣 浩幸, 吉良 龍太郎, 酒井 康成, 實藤 雅史, 武本 環美, 原 寿郎, 竹下 研三, 岩城 明子

    脳と発達   2005.5

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    PLP1遺伝子完全欠失によるPelizaeus-Merzbacher病(PLP null症候群)の男児例

  • ミトコンドリア病における中脳・橋背側部病変の持続性MRI拡散強調画像

    酒井 康成, 吉良 龍太郎, 鳥巣 浩幸, 原 寿郎

    脳と発達   2005.5

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    ミトコンドリア病における中脳・橋背側部病変の持続性MRI拡散強調画像

  • 幼児期より痙性麻痺が進行したKlinefelter症候群の1例

    實藤 雅文, 權藤 健二郎, 鳥巣 浩幸, 酒井 康成, 吉良 龍太郎, 原 寿郎, 松本 健一, 岩城 徹

    脳と発達   2005.3

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    幼児期より痙性麻痺が進行したKlinefelter症候群の1例

  • The gene expression profiles in peripheral blood mononuclear cells from patients with subacute sclerosing panencephalitis using oligonucleotide microarrays

    M Takemoto, R Kira, K Kusuhara, H Torisu, Y Sakai, T Hara

    FASEB JOURNAL   2005.3

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  • ウイルス感染と免疫 亜急性硬化性全脳炎の末梢血単核球における網羅的遺伝子発現解析

    武本 環美, 吉良 龍太郎, 楠原 浩一, 鳥巣 浩幸, 酒井 康成, 原 寿郎

    神経免疫学   2005.3

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    Language:Japanese  

    ウイルス感染と免疫 亜急性硬化性全脳炎の末梢血単核球における網羅的遺伝子発現解析

  • 亜急性硬化性全脳炎における末梢血単核球遺伝子発現プロファイル解析

    武本 環美, 吉良 龍太郎, 楠原 浩一, 鳥巣 浩幸, 酒井 康成, 原 寿郎

    日本小児科学会雑誌   2005.3

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    Language:Japanese  

    亜急性硬化性全脳炎における末梢血単核球遺伝子発現プロファイル解析

  • 乳児期発症Niemann-Pick病C型の1男児例

    鳥巣 浩幸, 實藤 雅文, 酒井 康成, 竹本 環美, 吉良 龍太郎, 原 寿郎

    脳と発達   2004.9

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    Language:Japanese  

    乳児期発症Niemann-Pick病C型の1男児例

  • Hyperornithinemia-hyperammonemiahomocitrullinuria(HHH)症候群の同胞例

    鳥巣 浩幸, 實藤 雅文, 酒井 康成, 武本 環美, 吉良 龍太郎, 原 寿郎

    脳と発達   2004.9

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    Language:Japanese  

    Hyperornithinemia-hyperammonemiahomocitrullinuria(HHH)症候群の同胞例

  • 亜急性硬化性全脳炎における遺伝子発現プロファイルとGranulysin遺伝子-189G/T多型解析

    武本 環美, 吉良 龍太郎, 鳥巣 浩幸, 酒井 康成, 原 寿郎

    脳と発達   2004.6

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    Language:Japanese  

    亜急性硬化性全脳炎における遺伝子発現プロファイルとGranulysin遺伝子-189G/T多型解析

  • Klinefelter症候群に合併した,脊髄前角細胞の著明な脱落を伴った脊髄小脳変性症の小児例

    實藤 雅文, 權藤 健二郎, 吉良 龍太郎, 鳥巣 浩幸, 酒井 康成, 原 寿郎

    脳と発達   2004.6

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    Language:Japanese  

    Klinefelter症候群に合併した,脊髄前角細胞の著明な脱落を伴った脊髄小脳変性症の小児例

  • MTH1は酸化ストレスによる細胞死を抑制する

    作見 邦彦, 吉村 大輔, 古市 正人, 酒井 康成, 大野 みずき, 岩井 成憲, 中別府 雄作

    日本癌学会総会記事   2003.8

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    Language:Japanese  

  • 単純型熱性けいれん感受性遺伝子の検討 Interleukin-1β遺伝子プロモーター多型は孤発例の発症に関与する

    吉良 龍太郎, 武本 環美, 實藤 雅文, 鳥巣 浩幸, 酒井 康成, 野村 明彦, 原 寿郎

    日本小児科学会雑誌   2003.6

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  • 亜急性硬化性全脳炎における抗ウイルス蛋白MxA遺伝子の解析

    鳥巣 浩幸, Bassuny Wafaa Mohamed, 實藤 雅文, 武本 環美, 酒井 康成, 吉良 龍太郎, 楠原 浩一, 原 寿郎

    日本小児科学会雑誌   2003.4

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    Language:Japanese  

  • マウス新規AP endonuclease候補遺伝子APE2のクローニングと遺伝子欠損ES細胞株の樹立

    井手康人, 冨永洋平, 土本大介, 酒井康成, 今磯泰幸, 中別府雄作

    日本分子生物学会年会プログラム・講演要旨集   2000.11

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    Language:Japanese  

    マウス新規AP endonuclease候補遺伝子APE2のクローニングと遺伝子欠損ES細胞株の樹立

  • インターフェロン-αが奏効した慢性活動性Epstein-Barrウイルス感染症の1例

    酒井 康成

    日本小児科学会雑誌   1997.5

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    Language:Japanese  

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Professional Memberships

  • The Molecular Biology Society of Japan

  • Society for Neuroscience

  • The American Society of Human Genetics

  • Japan Pediatric Society

  • The Japan Epilepsy Society

  • The Japan Society of Human Genetics

  • The Japanese Society of Child Neurology

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Committee Memberships

  • 日本人類遺伝学会   Councilor   Domestic

    2024.1 - 2024.5   

  • 日本てんかん学会   Councilor   Domestic

    2022.5 - 2027.4   

  • 日本小児神経学会   Councilor   Domestic

    2016.6 - 2017.6   

  • 福岡・久留米てんかん研究会   世話人   Domestic

    2013.6   

  • 福岡・久留米てんかん研究会   世話人   Domestic

    2013.6   

  • 福岡臨床と脳波懇話会   世話人   Domestic

    2013.4 - 2013.6   

  • 福岡小児神経研究会   世話人   Domestic

    2013.4 - 2013.6   

  • 日本小児神経学会九州地方会   世話人   Domestic

    2013.4 - 2013.6   

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Academic Activities

  • 脳と発達

    2019.5 - 2023.5

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    Type:Academic society, research group, etc. 

  • Screening of academic papers

    Role(s): Peer review

    2018

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:7

    Number of peer-reviewed articles in Japanese journals:7

    Proceedings of International Conference Number of peer-reviewed papers:0

    Proceedings of domestic conference Number of peer-reviewed papers:0

  • Screening of academic papers

    Role(s): Peer review

    2017

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:7

    Number of peer-reviewed articles in Japanese journals:0

    Proceedings of International Conference Number of peer-reviewed papers:0

    Proceedings of domestic conference Number of peer-reviewed papers:0

  • 会長

    第81回日本小児神経学会九州地方会  ( Japan ) 2016.8

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    Type:Competition, symposium, etc. 

    Number of participants:150

  • 会長

    第11回日本てんかん学会九州地方会  ( Japan ) 2016.7

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    Type:Competition, symposium, etc. 

    Number of participants:150

  • 世話人

    日本小児科学会福岡地方会  ( Japan ) 2014.12 - 2016.3

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    Type:Competition, symposium, etc. 

  • 世話人

    福岡小児神経研究会  ( Japan ) 2014.4 - 2016.3

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    Type:Competition, symposium, etc. 

  • 世話人

    日本小児神経学会九州地方会  ( Japan ) 2014.4 - 2016.3

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    日本小児科学会福岡地方会  ( Japan ) 2011.10

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    Type:Competition, symposium, etc. 

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Research Projects

  • MOG関連疾患の多様な臨床像を説明する分子パターンの解明

    Grant number:25K11060  2025.4 - 2029.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Chong Pin・Fee, 酒井 康成, 鳥巣 浩幸, 吉良 龍太郎

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    Grant type:Scientific research funding

    Myelin Oligodendrocyte Glycoprotein抗体関連疾患(MOGAD)は、小児期に比較的頻度の高い後天性中枢神経脱髄疾患である。MOGADの臨床スペクトラムは広く、初発時多彩な症状を示すことが知られている。しかし、MOG抗体(IgG)が自然免疫および獲得免疫システムの中で果たす病的意義はいまだに不明である。本研究に先立ち、我々はMOGAD患児脳脊髄液において特徴的な脂質・代謝プロファイル変化を示し、一部は髄液IL-17Aレベルと相関することを見出した。本研究では、MOG-IgG結合分子のプロテオーム解析を追加し、多彩なMOGADの臨床像に対応する多様な自己抗原を同定する。

    CiNii Research

  • 不均一かつ多様なミクログリア構成にもとづく脱髄性疾患の新規治療点の解明

    Grant number:23K07334  2023 - 2025

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    酒井 康成, 瀬戸山 大樹, 加藤 隆弘

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    Authorship:Principal investigator  Grant type:Scientific research funding

    小児の後天性脱髄性症候群(Acquired Demyelinating Syndrome: ADS)は、急性散在性脳脊髄炎、多発性硬化症、MOG抗体関連疾患など、多彩な臨床病型を含む疾患群の総称である。ADSの5-10%は15歳未満の小児に発症するが、若年発症に寄与する生物学的メカニズムは不明である。本研究では、ADS患児由来誘導脳オルガノイドおよびミクログリア(induced microglia-like cells: iMG)を用いた、新しいヒト脳疾患モデルを構築する。iMG内の遺伝子発現・代謝プロファイルを網羅的に解析し、臨床上有用な活動性指標および治療標的を抽出する。本研究を通して、小児ADSを克服するための分子医学的エビデンスを創出する。

    CiNii Research

  • 子どものいのちに関わる協働意思決定の実態調査研究~小児科医と家族の対話の構造化~

    Grant number:21K10293  2021 - 2023

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    笹月 桃子, 酒井 康成, 加部 一彦, 櫻井 浩子, 松岡 真里, 板井 孝一郎

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    Grant type:Scientific research funding

    重篤な病態を抱える子どものいのちに関わる医療の方針は、小児科医と家族の協働意思決定を通じて個別に見出される。その協働の実像である両者間の対話がどのような関係性の中で、いかなる言説で構築され、最終的に決定に至っているのか、それを現場から把握する意義は大きい。現場の意思決定支援の在り方が示唆され、それが子どもにとって最善の医療方針に還る。
    本研究では、我が国の小児医療現場における小児科医と家族の対話の実態を調査する。対話を通じて(1)交わされる言説(言葉と文脈)、(2)構築される関係性、(3)最終決定に至る過程、について、小児科医・家族・看護師の三者の視点から、質的・量的に明らかにする。

    CiNii Research

  • Shank3欠損マウスと患児オルガノイドを用いた視床・感覚路発達メカニズムの解析

    Grant number:21K07865  2021 - 2023

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    奥園 清香, 酒井 康成, 中別府 雄作, 康 東天

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    Grant type:Scientific research funding

    自閉スペクトラム症は社会的コミュニケーションの障害を特徴とする疾患であり、症状の一つに感覚の過敏性がある。私たちは自閉スペクトラム症の発症に関連するShank3というたんぱく質が、感覚神経の形成と発達に重要な役割を担っている可能性を見出した。この研究ではShank3のさらなる研究を通じて自閉スペクトラム症で感覚過敏性が起こる原因を明らかにし、治療薬の候補となるような薬剤の開発を目指している。

    CiNii Research

  • 皮質オルガノイドを用いたてんかん性脳症の収束的メカニズムおよび治療研究

    Grant number:19K08281  2019 - 2022

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • Detection of autoantibody in pediatric encephalitis

    Grant number:19K10613  2019 - 2022

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Chong Pin Fee

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    Grant type:Scientific research funding

    To classify etiologically unknown pediatric encephalitis cases systemically, we developed a research procedure employing a combination of cell-based assay and FilmArray meningitis/encephalitis panel. During the research period, in collaboration with medical institutions in Fukuoka prefecture, we were able to identify causative viruses in 5 cases, and NMDA receptor antibody in 6 cases from cerebrospinal fluid of pediatric encephalitis patients with unknown cause. We were unable to detect LGI1, CASPR2, GABAB receptor, and AMPA1/2 receptor antibodies as seen in adult patients. We also detected 15 cases of MOG antibodies in blood specimens, confirming the diagnosis of MOG antibody-positive central demyelinating disease. Analysis of lipid metabolism from cerebrospinal fluid specimens of MOG antibody-positive patients revealed a metabolic profile that differed from that of controls.

    CiNii Research

  • 皮質オルガノイドを用いたてんかん性脳症の収束的メカニズムおよび治療研究

    Grant number:19K08281  2019 - 2021

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • 患者由来直接誘導ニューロン・グリア細胞による精神神経疾患の病態解明:橋渡し研究

    Grant number:18H04042  2018 - 2021

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    加藤 隆弘, 酒井 康成, 葛巻 直子, 吉川 武男

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    Grant type:Scientific research funding

    本研究では様々な精神疾患患者から疾患モデル細胞としてiMG細胞及びiN細胞を作製し、生きた細胞でしか評価しえない項目を測定し、臨床症状との相関解析などにより、疾患特異性を検出し、ダイナミックな細胞レベルでの病態解明を進めてきた。発達障害および認知症関連の患者由来のiN細胞およびiMG細胞において特異的な反応特性を見出した。さらに、ヒト血液由来iMG細胞がヒト脳ミクログリア細胞と類似することを見出し、iMG細胞のサロゲート細胞としての有用性を明らかにした。

    CiNii Research

  • 今回の研究では、川崎病患者105例の血清のリピドミクス解析を行い、川崎病関連28物質を同定した。

    2018 - 2019

    Grants-in-Aid for Scientific Research  Grants-in-Aid for Scientific Research (Ministry of Health, Labour and Welfare)

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    Authorship:Coinvestigator(s)  Grant type:Competitive funding other than Grants-in-Aid for Scientific Research

  • 先天性門脈体循環シャント症候群を引き起こす遺伝的要因の解明

    Grant number:17K10146  2017 - 2020

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • 小児の脱髄性疾患の実態調査、診断ガイドラインの確立

    2017 - 2019

    Grants-in-Aid for Scientific Research  Grants-in-Aid for Scientific Research (Ministry of Health, Labour and Welfare)

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    Authorship:Coinvestigator(s)  Grant type:Competitive funding other than Grants-in-Aid for Scientific Research

  • 研究助成「誘導皮質スフェロイドを用いた早期発症型てんかん性脳症の発症メカニズムと新規治療法の探索」

    2017

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    Grant type:Donation

  • 重篤な疾患や障害をもつ子どもの延命に関わる治療方針決定について

    Grant number:15K08555  2015 - 2017

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • Pten変異マウスを用いた新しいてんかん病理・治療モデルの確立

    Grant number:15K09624  2015 - 2017

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • Pten変異マウスを用いた新しいてんかん病理・治療モデルの確立

    Grant number:15K09624  2015 - 2017

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 小児の脱髄性疾患の実態調査、診断ガイドラインの確立

    2015 - 2016

    Grants-in-Aid for Scientific Research  Grants-in-Aid for Scientific Research (Ministry of Health, Labour and Welfare)

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    Authorship:Coinvestigator(s)  Grant type:Competitive funding other than Grants-in-Aid for Scientific Research

  • 研究助成「てんかん性脳症に関与する収束的分子シグナルの解明」

    2015

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    Grant type:Donation

  • エキソーム解析と患者iPSC由来神経細胞を用いたSSPEの包括的な病態解析

    Grant number:26461547  2014 - 2016

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • 小児医学研究助成「SHANK3を中心とする自閉症関連分子シグナルの探索」

    2013

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    Grant type:Donation

  • 精神遅滞関連タンパク質複合体による生後脳発達の制御メカニズムに関する研究

    Grant number:24650199  2012 - 2014

    Grants-in-Aid for Scientific Research  Grant-in-Aid for challenging Exploratory Research

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 精神遅滞関連タンパク質複合体による生後脳発達の制御メカニズムに関する研究

    Grant number:24650199  2012 - 2014

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • 研究助成「自閉症モデルにおける脳内シグナル伝達異常に関する研究」

    2012

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    Grant type:Donation

  • プロテオミクスによるウイルス関連脳症の病態特異的バイオマーカーの探索

    Grant number:23591503  2011 - 2013

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • 日本人自閉症児に見られる新規染色体コピー数変異の探索

    Grant number:23810025  2011 - 2012

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (Start-up)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 日本人自閉症児に見られる新規染色体コピー数変異の探索

    Grant number:23810025  2011 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • 研究助成金「PTEN遺伝子変異にともなう脳内トランスクリプトーム変化と治療標的の探索」

    2011

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    Grant type:Donation

  • 急性散在性脳脊髄炎における分子遺伝学的病態解析

    Grant number:17591095  2005 - 2007

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • ナノ粒子を用いた遺伝子治療法の開発

    Grant number:16659279  2004 - 2005

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • 右脳・左脳の包括的遺伝子解析による高次機能発達機構の解明

    Grant number:16591038  2004 - 2005

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • 細胞内寄生性細菌に対する宿主反応の解析と細胞・遺伝子治療法の開発

    Grant number:15390325  2003 - 2005

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant type:Scientific research funding

  • 次世代遺伝子治療の開発

    2002.4 - 2005.3

    Joint research

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    Authorship:Coinvestigator(s)  Grant type:Other funds from industry-academia collaboration

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Educational Activities

  • 医学部2年生講義「基礎遺伝学」
    医学部4年生講義「臨床遺伝学」「神経代謝疾患」
    大学院生研究指導
    基礎配属学部学生の研究指導
    教室カンファレンス・若手小児科医指導

Class subject

  • 小児神経学

    2023.4 - 2023.9   First semester

  • 遺伝学

    2023.4 - 2023.9   First semester

  • 臨床遺伝学

    2020.10 - 2021.3   Second semester

  • 基礎遺伝学

    2020.4 - 2020.9   First semester

  • 生命医科学入門

    2016.10 - 2017.3   Second semester

  • 臨床遺伝学

    2016.10 - 2017.3   Second semester

  • 基礎遺伝学

    2016.4 - 2016.9   First semester

  • 生命医科学入門

    2015.10 - 2016.3   Second semester

  • 基礎遺伝学

    2015.4 - 2015.9   First semester

  • 臨床遺伝学

    2015.4 - 2015.9   First semester

  • 臨床遺伝学

    2014.10 - 2015.3   Second semester

  • 小児科抄読会

    2014.4 - 2015.3   Full year

  • 小児神経学・代謝2

    2014.4 - 2015.3   Full year

  • 基礎配属・実習

    2014.4 - 2014.9   First semester

  • 臨床遺伝学

    2013.10 - 2014.3   Second semester

  • 小児科抄読会

    2013.4 - 2014.3   Full year

  • 基礎配属・実習

    2013.4 - 2013.9   First semester

  • 臨床遺伝学

    2012.10 - 2013.3   Second semester

  • 小児科抄読会

    2012.4 - 2013.3   Full year

  • 基礎配属・実習

    2012.4 - 2012.9   First semester

  • 基礎遺伝学

    2011.10 - 2012.3   Second semester

  • 臨床遺伝学

    2011.10 - 2012.3   Second semester

  • 基礎配属・実習

    2011.4 - 2011.9   First semester

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Other educational activity and Special note

  • 2011  Class Teacher  学部

Social Activities

  • 教育講演「小児期のてんかんと学校生活」

    日本学校保健学会  大分市  2018.12

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 九州大学病院,学外病院での小児神経外来を担当 福岡・糸島・田川地区急患センターにて休日夜間診療

    2016

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    九州大学病院,学外病院での小児神経外来を担当
    福岡・糸島・田川地区急患センターにて休日夜間診療

  • 九州大学病院,学外病院での小児神経外来を担当. 福岡・糸島地区急患センターにて休日夜間診療.

    2015

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    九州大学病院,学外病院での小児神経外来を担当.
    福岡・糸島地区急患センターにて休日夜間診療.

  • 九州大学病院,学外病院での小児神経外来を担当. 福岡・糸島地区急患センターにて休日夜間診療.

    2014

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    九州大学病院,学外病院での小児神経外来を担当.
    福岡・糸島地区急患センターにて休日夜間診療.

  • 教育講演「自閉症の多様な遺伝的背景をつなぐ相互作用ネットワーク」

    日本小児科学会・福岡地方会事務局(福岡大学小児科)  福岡大学  2012.2

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

    1980年代の双生児疫学研究を起点として,自閉症の遺伝学的要因が注目されるようになった.さらに近年,全ゲノム解析技術の発展に伴い,自閉症関連領域が次々に報告され,自閉症をめぐる遺伝学的パラダイムはここ数年間で大きく変化した.一方,複雑かつ多岐にわたる自閉症の遺伝的要因が,どのような共通経路を経て自閉症固有の臨床徴候をもたらすのかといった,生物学的理解が今後の課題となった.自閉症形質の共通メカニズムを解明するために,私は米国Huda Zoghbi研究室において,35の自閉症関連遺伝子に対する結合パートナーの大規模スクリーニングを行った.得られたタンパク質間相互作用ネットワーク(自閉症インタラクトーム)は,異なる自閉症関連タンパク質が互いに近接する機能的関連性を示すことを支持した.自閉症インタラクトームのin vivoおよび生理学的な条件との整合性は,マウス脳を用いた生化学的解析によって確定した.また自閉症児に見いだされたコピー数変異領域内の遺伝子を解析することにより,本ネットワークと自閉症の遺伝的要因との関連性を裏付けた.以上,本ネットワークは,多様な遺伝的要因を有する個々の自閉症に対して収束的メカニズムを捉え,また新たな治療標的を探索する上で有用な研究基盤になることを示す.

  • 九州大学病院,学外病院での小児神経外来を担当. 福岡・糸島地区急患センターにて休日夜間診療.

    2004

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    九州大学病院,学外病院での小児神経外来を担当.
    福岡・糸島地区急患センターにて休日夜間診療.

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Media Coverage

  • ヤオコー会長 川野幸夫氏 インタビュー記事(川野賞受賞時の写真掲載) Newspaper, magazine

    日経MJ  2019.6

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    ヤオコー会長 川野幸夫氏 インタビュー記事(川野賞受賞時の写真掲載)

Travel Abroad

  • 2006.4 - 2011.3

    Staying countory name 1:United States   Staying institution name 1:Howard Hughes Medical Institute, Department of Molecular and Human Genetics, Baylor College of Medicine (The laboratory of Dr. Huda Y Zoghbi)

Specialized clinical area

  • Biology / Medicine, Dentistry and Pharmacy / Internal Medicine / Pediatrics

    小児神経学

Clinician qualification

  • Specialist

    The Japanese Society of Child Neurology

  • Preceptor

    Japan Pediatric Society

Year of medical license acquisition

  • 1995