記述言語：日本語   掲載種別：研究論文（学術雑誌）  

掲載種別：研究論文（学術雑誌）  

掲載種別：研究論文（学術雑誌）  

掲載種別：研究論文（学術雑誌）  

掲載種別：研究論文（学術雑誌）  

掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：英語   出版者・発行元：EMBO Reports  

Adult neural stem cells (NSCs) in the hippocampal dentate gyrus continuously proliferate and generate new neurons throughout life. Although various functions of organelles are closely related to the regulation of adult neurogenesis, the role of endoplasmic reticulum (ER)-related molecules in this process remains largely unexplored. Here we show that Derlin-1, an ER-associated degradation component, spatiotemporally maintains adult hippocampal neurogenesis through a mechanism distinct from its established role as an ER quality controller. Derlin-1 deficiency in the mouse central nervous system leads to the ectopic localization of newborn neurons and impairs NSC transition from active to quiescent states, resulting in early depletion of hippocampal NSCs. As a result, Derlin-1-deficient mice exhibit phenotypes of increased seizure susceptibility and cognitive dysfunction. Reduced Stat5b expression is responsible for adult neurogenesis defects in Derlin-1-deficient NSCs. Inhibition of histone deacetylase activity effectively induces Stat5b expression and restores abnormal adult neurogenesis, resulting in improved seizure susceptibility and cognitive dysfunction in Derlin-1-deficient mice. Our findings indicate that the Derlin-1-Stat5b axis is indispensable for the homeostasis of adult hippocampal neurogenesis.

DOI： 10.1038/s44319-024-00205-7

Web of Science

Scopus

PubMed

記述言語：英語   出版者・発行元：Neural Regeneration Research  

DOI： 10.4103/1673-5374.382240

Web of Science

Scopus

PubMed

記述言語：英語   出版者・発行元：Cells  

Although lineage reprogramming from one cell type to another is becoming a breakthrough technology for cell-based therapy, several limitations remain to be overcome, including the low conversion efficiency and subtype specificity. To address these, many studies have been conducted using genetics, chemistry, physics, and cell biology to control transcriptional networks, signaling cascades, and epigenetic modifications during reprogramming. Here, we summarize recent advances in cellular reprogramming and discuss future directions.

DOI： 10.3390/cells13080707

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PubMed

記述言語：英語   出版者・発行元：Proceedings of the National Academy of Sciences of the United States of America  

Although generating new neurons in the ischemic injured brain would be an ideal approach to replenish the lost neurons for repairing the damage, the adult mammalian brain retains only limited neurogenic capability. Here, we show that direct conversion of microglia/macrophages into neurons in the brain has great potential as a therapeutic strategy for ischemic brain injury. After transient middle cerebral artery occlusion in adult mice, microglia/macrophages converge at the lesion core of the striatum, where neuronal loss is prominent. Targeted expression of a neurogenic transcription factor, NeuroD1, in microglia/macrophages in the injured striatum enables their conversion into induced neuronal cells that functionally integrate into the existing neuronal circuits. Furthermore, NeuroD1-mediated induced neuronal cell generation significantly improves neurological function in the mouse stroke model, and ablation of these cells abolishes the gained functional recovery. Our findings thus demonstrate that neuronal conversion contributes directly to functional recovery after stroke.

DOI： 10.1073/pnas.2307972120

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PubMed

記述言語：英語   出版者・発行元：Genes to Cells  

Neuronal regeneration to replenish lost neurons after injury is critical for brain repair. Microglia, brain-resident macrophages that have the propensity to accumulate at the site of injury, can be a potential source for replenishing lost neurons through fate conversion into neurons, induced by forced expression of neuronal lineage-specific transcription factors. However, it has not been strictly demonstrated that microglia, rather than central nervous system-associated macrophages, such as meningeal macrophages, convert into neurons. Here, we show that NeuroD1-transduced microglia can be successfully converted into neurons in vitro using lineage-mapping strategies. We also found that a chemical cocktail treatment further promoted NeuroD1-induced microglia-to-neuron conversion. NeuroD1 with loss-of-function mutation, on the other hand, failed to induce the neuronal conversion. Our results indicate that microglia are indeed reprogrammed into neurons by NeuroD1 with neurogenic transcriptional activity.

DOI： 10.1111/gtc.13033

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PubMed

出版者・発行元：医歯薬出版  

DOI： 10.32118/ayu28411869

CiNii Research

記述言語：日本語   出版者・発行元：日本毒性学会  

<p>麻酔への早期ライフ曝露とその後の学習障害との関連は、子供たちとその家族にとって大きな関心事である。我々は、小児麻酔薬として広く用いられているミダゾラム（MDZ）の早期ライフ曝露により、マウス海馬の神経幹細胞（NSC）のクロマチンアクセシビリティおよび静止関連遺伝子の発現が持続的に変化することを明らかにした。この変化は、成体になっても持続的にNSCの増殖を制限し、その結果、ニューロン新生が減少し、海馬依存性の記憶機能の障害と関連することを明らかにした。さらに、自発的な運動は海馬のニューロン新生を回復させ、MDZで障害された遺伝子発現を正常化し、MDZ曝露マウスの認知能力を改善することを見いだした。これらの結果は、小児麻酔が脳機能に長期的な悪影響を及ぼす原因を説明し、その対策として可能な治療法を提供するものである。</p>

DOI： 10.14869/toxpt.50.1.0_s31-1

CiNii Research

記述言語：英語   出版者・発行元：Scientific Reports  

Several transcription factors, including NeuroD1, have been shown to act as neuronal reprogramming factors (RFs) that induce neuronal conversion from somatic cells. However, it remains unexplored whether expression levels of RFs in the original cells affect reprogramming efficiency. Here, we show that the neuronal reprogramming efficiency from two distinct glial cell types, microglia and astrocytes, is substantially dependent on the expression level of NeuroD1: low expression failed to induce neuronal reprogramming, whereas elevated NeuroD1 expression dramatically improved reprogramming efficiency in both cell types. Moreover, even under conditions where NeuroD1 expression was too low to induce effective conversion by itself, combined expression of three RFs (Ascl1, Brn2, and NeuroD1) facilitated the breaking down of cellular barriers, inducing neuronal reprogramming. Thus, our results suggest that a sufficiently high expression level of RFs, or alternatively their combinatorial expression, is the key to achieving efficient neuronal reprogramming from different cells.

DOI： 10.1038/s41598-022-22802-z

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PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1080/23262133.2015.1081714

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.ijdevneu.2013.02.006