Updated on 2024/10/07

Information

 

写真a

 
KAMAKURA SACHIKO
 
Organization
Faculty of Medical Sciences Research Center for Human Disease Modeling Lecturer
School of Medicine Department of Medicine(Joint Appointment)
Title
Lecturer
Profile
研究活動:好中球ケモタキシスにおける細胞極性制御タンパク質の役割、上皮細胞の細胞極性制御機構
External link

Degree

  • Ph.D

Research History

  • 東京大学 博士研究員

Research Interests・Research Keywords

  • Research theme:Mechanisms for formation of protein complexes involved in cell polarity establishment

    Keyword:polarity, epithelial cells, neutrophil, chemotaxis

    Research period: 2004.4

Papers

  • The NADPH oxidases DUOX1 and DUOX2 are sorted to the apical plasma membrane in epithelial cells via their respective maturation factors DUOXA1 and DUOXA2

    Kohda, A; Kamakura, S; Hayase, J; Sumimoto, H

    GENES TO CELLS   2024.8   ISSN:1356-9597 eISSN:1365-2443

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    Language:English   Publisher:Genes to Cells  

    The membrane-integrated NADPH oxidases DUOX1 and DUOX2 are recruited to the apical plasma membrane in epithelial cells to release hydrogen peroxide, thereby playing crucial roles in various functions such as thyroid hormone synthesis and host defense. However, it has remained unknown about the molecular mechanism for apical sorting of DUOX1 and DUOX2. Here we show that DUOX1 and DUOX2 are correctly sorted to the apical membrane via the membrane-spanning DUOX maturation proteins DUOXA1 and DUOXA2, respectively, when co-expressed in MDCK epithelial cells. Impairment of N-glycosylation of DUOXA1 results in mistargeting of DUOX1 to the basolateral membrane. Similar to DUOX1 complexed with the glycosylation-defective DUOXA1, the naturally non-glycosylated oxidase NOX5, which forms a homo-oligomer, is targeted basolaterally. On the other hand, a mutant DUOXA2 deficient in N-glycosylation is less stable than the wild-type protein but still capable of recruiting DUOX2 to the apical membrane, whereas DUOX2 is missorted to the basolateral membrane when paired with DUOXA1. These findings indicate that DUOXA2 is crucial but its N-glycosylation is dispensable for DUOX2 apical recruitment; instead, its C-terminal region seems to be involved. Thus, apical sorting of DUOX1 and DUOX2 is likely regulated in a distinct manner by their respective partners DUOXA1 and DUOXA2.

    DOI: 10.1111/gtc.13153

    Web of Science

    Scopus

    PubMed

  • TMEM25 is a Par3-binding protein that attenuates claudin assembly during tight junction development Reviewed International journal

    Kamakura S, Hayase J, Kohda A, Iwakiri Y, Chishiki K, Izaki T & Sumimoto H.

    EMBO reports   25   144 - 167   2024.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s44319-023-00018-0

  • Hypoxia stabilizes the H<sub>2</sub>O<sub>2</sub>-producing oxidase Nox4 in cardiomyocytes via suppressing autophagy-related lysosomal degradation

    Matsunaga, S; Kohda, A; Kamakura, S; Hayase, J; Miyano, K; Shiose, A; Sumimoto, H

    GENES TO CELLS   29 ( 1 )   63 - 72   2024.1   ISSN:1356-9597 eISSN:1365-2443

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    Language:English   Publisher:Genes to Cells  

    The hydrogen peroxide (H2O2)-producing NADPH oxidase Nox4, forming a heterodimer with p22phox, is expressed in a variety of cells including those in the heart to mediate adaptive responses to cellular stresses such as hypoxia. Since Nox4 is constitutively active, H2O2 production is controlled by its protein abundance. Hypoxia-induced Nox4 expression is observed in various types of cells and generally thought to be regulated at the transcriptional level. Here we show that hypoxia upregulates the Nox4 protein level and Nox4-catalyzed H2O2 production without increasing the Nox4 mRNA in rat H9c2 cardiomyocytes. In these cells, the Nox4 protein is stabilized under hypoxic conditions in a manner dependent on the presence of p22phox. Cell treatment with the proteasome inhibitor MG132 results in a marked decrease of the Nox4 protein under both normoxic and hypoxic conditions, indicating that the proteasome pathway does not play a major role in Nox4 degradation. The decrease is partially restored by the autophagy inhibitor 3-methyladenine. Furthermore, the Nox4 protein level is upregulated by the lysosome inhibitors bafilomycin A1 and chloroquine. Thus, in cardiomyocytes, Nox4 appears to be degraded via an autophagy-related pathway, and its suppression by hypoxia likely stabilizes Nox4, leading to upregulation of Nox4-catalyzed H2O2 production.

    DOI: 10.1111/gtc.13085

    Web of Science

    Scopus

    PubMed

  • GPR125 (ADGRA3) is an autocleavable adhesion GPCR that traffics with Dlg1 to the basolateral membrane and regulates epithelial apicobasal polarity Reviewed International journal

    J Biol Chem.   298 ( 10 )   102475   2022.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jbc.2022.102475.

  • Hepatocyte polarity establishment and apical lumen formation are organized by Par3, Cdc42, and aPKC in conjunction with Lgl Reviewed International journal

    Tocan V, Hayase J, Kamakura S, Kohda A, Ohga S, Kohjima M, Sumimoto H.

    J. Biol. Chem.   297 ( 6 )   101354   2021.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: doi: 10.1016/j.jbc.2021.101354

  • Intramolecular interaction in LGN, an adaptor protein that regulates mitotic spindle orientation. Reviewed International journal

    Takayanagi H, Hayase J, Kamakura S, Miyano K, Chishiki K, Yuzawa S, Sumimoto H.

    J. Biol. Chem.   294   19655 - 19666   2019.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.RA119.011457

  • Differential cell surface recruitment of the superoxide-producing NADPH oxidases Nox1, Nox2 and Nox5: The role of the small GTPase Sar1. Reviewed International journal

    Kiyohara T, Miyano K, Kamakura S, Hayase J, Chishiki K, Kohda A, Sumimoto H.

    Genes Cells.   23 ( 6 )   480 - 493   2018.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/gtc.12590

  • Ric-8A, an activator protein of Gαi, controls mammalian epithelial cell polarity for tight junction assembly and cystogenesis Reviewed International journal

    22 ( 3 )   293 - 309   2017.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/gtc.12477

  • Ric-8A-mediated stabilization of the trimeric G protein subunit Gαi is inhibited by pertussis toxin-catalyzed ADP-ribosylation Reviewed International journal

    483 ( 3 )   941 - 945   2017.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbrc.2017.01.036.

  • c-Rel Regulates Inscuteable Gene Expression during Mouse Embryonic Stem Cell Differentiation. Reviewed International journal

    Ishibashi R, Kozuki S, Kamakura S, Sumimoto H, Toyoshima F.

    J. Biol. Chem.   291 ( 7 )   3333 - 3345   2016.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M115.679563

  • Structural basis of cofactor-mediated stabilization and substrate recognition of the α-tubulin acetyltransferase αTAT1. Reviewed International journal

    ( 467 )   103 - 113   2015.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1042/BJ20141193

  • The Cell Polarity Protein mInsc Regulates Neutrophil Chemotaxis via a Noncanonical G Protein Signaling Pathway. Reviewed International journal

    Sachiko Kamakura, Masatoshi Nomura, Junya Hayase, Yuko Iwakiri, Akihiko Nishikimi, Yoshinori Fukui, Ryoichi Takayanagi, Hideki Sumimoto

    Developmental Cell   ( 26 )   292 - 302   2013.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.devcel.2013.06.008.

  • Ubiquitination of the heterotrimeric G protein α subunits Gαi2 and Gαq is prevented by the guanine nucleotide exchange factor Ric-8A. Reviewed International journal

    435 ( 3 )   414 - 419   2013.6

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Interaction of NuMA protein with the kinesin Eg5: its possible role in bipolar spindle assembly and chromosome alignment. Reviewed International journal

    Yuko Iwakiri, Sachiko Kamakura, Junya Hayase, Hideki Sumimoto

    Biochem J.   451 ( 2 )   195 - 204   2013.4

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    Language:English   Publishing type:Research paper (scientific journal)  

  • The WD40 protein Morg1 facilitates Par6-aPKC binding to Crb3 for apical identity in epithelial cells. Reviewed International journal

    Junya Hayase, Sachiko Kamakura, Yuko Iwakiri, Yoshihiro Yamaguchi, Tomoko Izaki, Takashi Ito, Hideki Sumimoto

    J. Cell Biol.   200 ( 5 )   635 - 650   2013.3

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Structural basis for interaction between the conserved cell polarity proteins Inscuteable and Leu-Gly-Asn repeat-enriched protein (LGN). Reviewed International journal

    Yuzawa S, Kamakura S, Iwakiri Y, Hayase J, Sumimoto H.

    Proc Natl Acad Sci U S A.   108 ( 48 )   2011.11

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Polarity proteins Bem1 and Cdc24 are components of the filamentous fungal NADPH oxidase complex. Reviewed International journal

    Takemoto, D., Kamakura, S., Saikia, S., Becker, Y., Wrenn, R., Tanaka, A., Sumimoto, H., Scott, B.

    Proc. Natl. Acad. Sci. USA   108   2011.7

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Membrane phospholipid metabolism during phagocytosis in human neutrophils. Reviewed International journal

    Minakami, R., Maehara, Y., Kamakura, S., Kumano, O., Miyano, K., Sumioto, H

    Genes Cells   15   2010.4

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Full-length p40phox structure suggests a basis for regulation mechanism of its membrane binding Reviewed International journal

    Honbou K, Minakami R, Yuzawa S, Takeya R, Suzuki NN, Kamakura S, Sumimoto H, Inagaki F.

    EMBO J.   2007.2

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  • Two forms of human Inscuteable-related protein that links Par3 to the Pins homologues LGN and AGS3 Reviewed International journal

    Izaki, T., Kamakura, S., Kohjima, M., and Sumimoto, H.

    Biochem. Biophys. Res. Commun.   2006.1

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Phosphorylation-dependent binding of 14-3-3 to Par3beta, a human Par3-related cell polarity protein Reviewed International journal

    T. Izaki, S. Kamakura, M. Kohjima and H. Sumimoto,

    Biochem. Biophys. Res. Commun.   329 ( 1 )   211 - 218   2005.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbrc.2005.01.115

  • Notch promotes survival of neural precursor cells via mechanisms distinct from those regulating neurogenesis. Reviewed International journal

    Koji Oishi, Sachiko Kamakura, Yuko Isazawa, Takeshi Yoshimatsu, Keisuke Kuida, Masato Nakafuku, Norihisa Masuyama, Yukiko Gotoh

    Dev. Biol.   2004.1

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Hes binding to STAT3 mediates crosstalk between Notch and JAK-STAT signaling. Reviewed International journal

    Sachiko Kamakura, Koji Oishi, Takeshi Yoshimatsu, Masato Nakafuku, Norihisa Masuyama, Yukiko Gotoh

    Nature Cell Biol.   2004.1

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Distinct Domains of Mouse Dishevelled Are Responsible for the c-Jun N-terminal Kinase/Stress-activated Protein Kinase Activation and the Axis Formation in Vertebrates. Reviewed International journal

    Tetsuo Moriguchi, Kaoru Kawachi, Sachiko Kamakura, Norihisa Masuyama, Hiroaki Yamanaka, Kunihiro Matsumoto, Akira Kikuchi, Eisuke Nishida

    J. Biol. Chem.   274   30957 - 30962   1999.10

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Activation of the Protein Kinase ERK5/BMK1 by Receptor Tyrosine Kinases Reviewed International journal

    Sachiko Kamakura, Tetsuo Moriguchi, Eisuke Nishida

    J. Biol. Chem.   1999.1

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  • Cloning and Characterization of the 5'-Flanking Region of the Human Dopamine D4 Receptor Gene. Reviewed International journal

    Sachiko Kamakura, Akiko Iwaki, Mitsuyuki Matsumoto, Yasuyuki Fukumaki

    Biochem. Biophys. Res. Commun.   235 ( 2 )   321 - 326   1997.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1006/bbrc.1997.6770

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Books

  • The NADPH oxidase activator p67phox and its related proteins. In: Pick, E. (ed.) NADPH Oxidases Revisited: From Function to Structure.

    Sumimoto, H., Kohda, A., Hayase, J., Kamakura, S.( Role: Joint author)

    Springer, Cham.  2023.6 

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    Language:Japanese   Book type:Scholarly book

    DOI: 10.1007/978-3-031-23752-2_16

  • Vascular Biology Navigator

    ( Role: Joint author)

    2001.1 

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    Responsible for pages:MAP キナーゼ   Language:Japanese   Book type:Scholarly book

  • バイオサイエンスの新世紀9:シグナル伝達(西田栄介、大野茂男 編)

    鎌倉幸子、西田栄介( Role: Joint author)

    共立出版  2001.1 

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    Responsible for pages:第6章MAP キナーゼI—総論:分子および細胞レベル   Language:Japanese   Book type:Scholarly book

Presentations

  • 細胞極性制御因子によるtight junction形成の制御機構 Invited

    鎌倉 幸子、住本 英樹

    第96回日本生化学会大会  2023.11 

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    Event date: 2023.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:福岡国際会議場   Country:Japan  

  • 新規Par3結合タンパク質によるタイトジャンクション形成の制御機構

    鎌倉 幸子、早瀬 純也、住本 英樹

    令和2年度日本生化学会 九州支部例会  2020.5 

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    Event date: 2020.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学   Country:Japan  

  • aPKC による上皮細胞再極性化メカニズムの解析

    早瀬純也、鎌倉幸⼦、住本英樹

    令和2年度日本生化学会 九州支部例会  2020.5 

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    Event date: 2020.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学   Country:Japan  

  • 好中球の遊走方向を制御するGPCRからの新しいシグナリング経路 Invited

    鎌倉 幸子, 住本 英樹

    第27回 日本生体防御学会学術総会  2016.7 

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    Event date: 2016.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 百日咳毒素はGαiとRic-8Aの結合を阻害することによりGαiのタンパク質レベルを減少させる

    知識 嘉奈子, 鎌倉 幸子, 早瀬 純也, 住本 英樹

    BMB2015 (第38回 日本分子生物学会年会・   第88回 日本生化学会大会 合同大会)  2015.12 

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    Event date: 2015.12

    Language:Japanese  

    Country:Japan  

  • Noncanonical G protein signaling regulates neutrophil chemotaxis via the cell polarity protein mInsc Invited International conference

    Sachiko Kamakura, Masatoshi Nomura, Junya Hayase, Yuko Iwakiri, Akihiko Nishikimi, Ryoichi Takayanagi, Yoshinori Fukui, and Hideki Sumimoto

    2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • ヒトドーパミン受容体D4遺伝子のプロモーター領域の解析

    第20回日本分子生物学会年会  1997.1 

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    Country:Japan  

  • MAPキナーゼファミリー分子ERK5のシグナル伝達経路の解析

    第21回日本分子生物学会年会  1998.1 

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    Country:Japan  

  • 哺乳類神経系前駆細胞の生存維持機構の解析

    第23回日本分子生物学会年会  2001.1 

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    Country:Japan  

  • 哺乳類神経系前駆細胞の生存維持機構の解析

    第54回日本細胞生物学会大会  2001.1 

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    Country:Japan  

  • 神経系前駆細胞におけるNotchシグナル伝達経路の解析

    第25日本分子生物学会年会  2002.1 

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    Country:Japan  

  • 神経系前駆細胞におけるNotchシグナル伝達経路の解析

    第56回日本細胞生物学会大会 ワークショップ  2003.1 

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    Country:Japan  

  • 神経系前駆細胞の自己複製制御機構の解析 Invited

    鎌倉幸子 後藤由季子

    第24回日本炎症再生医学会 ワークショップ  2003.1 

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    Country:Japan  

  • Human Inscuteable-related protein links Par3 to the Pins homologues LGN and AGS3 to regulate cell polarity International conference

    Sachiko Kamakura, Tomoko Izaki, Motoyuki Kohjim, and Hideki Sumimoto

    2006.6 

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    Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 好中球ケモタキシスにおける遊走方向制御の分子機構 Invited

    鎌倉 幸子、住本 英樹

    平成26年度 日本生化学会九州支部会例会  2014.5 

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    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:福岡   Country:Japan  

  • GPCRからの新しいシグナリング経路:白血球の遊走制御機構 Invited

    鎌倉 幸子、野村 政壽、早瀬 純也、岩切 優子、錦見 昭彦、高柳 涼一、福井 宣規、住本 英樹

    第87回日本内分泌学会学術総会  2014.4 

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    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:福岡   Country:Japan  

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MISC

  • 神経幹細胞の運命を制御するNotch-Hes経路とJAK-STAT  経路のクロストーク

    鎌倉幸子、吉松剛志、後藤由季子

    実験医学 羊土社   2004.10

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • 神経系前駆細胞の生存と死の制御

    鎌倉幸子 後藤由季子

    神経研究の進歩、 医学書院   2002.1

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • MAP キナーゼスーパーファミリーのシグナル伝達とその機能

    鎌倉幸子、西田栄介

    Biotherapy、癌と化学療法社   1998.1

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

Academic Activities

  • オーガナイザー

    第96回日本生化学会大会  ( 福岡国際会議場 ) 2023.11

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    Type:Competition, symposium, etc. 

Research Projects

  • 上皮形態形成を制御する細胞極性制御因子複合体の作用機構

    Grant number:22K06901  2022 - 2024

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 財団法人日本ワックスマン財団 学術研究助成金/好中球の遊走方向を制御するGPCR下流の新規シグナル伝達経路の解明

    2019

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    Grant type:Donation

  • 細胞極性蛋白質複合体によるタイトジャンクション形成の制御機構

    Grant number:18K06934  2018 - 2020

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 白血球の遊走方向を制御する細胞極性蛋白質複合体の作用機構

    Grant number:15K08309  2015 - 2017

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 白血球の遊走方向を決める細胞極性蛋白質複合体の作用機構

    Grant number:24590385  2012 - 2014

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 細胞極性蛋白質複合体による白血球の遊走方向決定機構

    Grant number:22790321  2010 - 2011

    科学研究費助成事業  若手研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 細胞極性決定に関与する膜直下シグナリング複合体の形成機構

    Grant number:19036019  2008 - 2009

    科学研究費助成事業  特定領域研究

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 細胞極性を制御する新規Par3結合蛋白質の作用機構

    Grant number:20790233  2008 - 2009

    科学研究費助成事業  若手研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 細胞極性決定に関与する膜直下シグナリング複合体の形成機構

    Grant number:19036019  2007 - 2008

    科学研究費助成事業  特定領域研究

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 哺乳類細胞の極性決定時におけるG蛋白質の役割とその制御機構

    Grant number:18057016  2006 - 2007

    科学研究費助成事業  特定領域研究

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 細胞極性決定に関する膜直下シグナリング複合体の形成機構

    Grant number:17048019  2005 - 2006

    科学研究費助成事業  特定領域研究

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 哺乳類神経幹細胞の分化制御における細胞極性タンパク質の役割

    Grant number:17790194  2005 - 2006

    科学研究費助成事業  若手研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

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Class subject

  • 人体構造と機能Ⅲ

    2023.4 - 2023.9   First semester

  • 生化学実習

    2022.10 - 2023.3   Second semester

  • 生化学研究法

    2022.4 - 2023.3   Full year

  • 人体構造と機能Ⅲ

    2022.4 - 2022.9   First semester

  • 生化学実習

    2021.10 - 2022.3   Second semester

  • 生化学研究法

    2021.4 - 2022.3   Full year

  • 人体構造と機能Ⅲ

    2021.4 - 2021.9   First semester

  • 生化学実習

    2020.10 - 2021.3   Second semester

  • 生化学研究法

    2020.4 - 2021.3   Full year

  • 人体構造と機能Ⅲ

    2020.4 - 2020.9   First semester

  • 生化学実習

    2019.10 - 2020.3   Second semester

  • 生化学研究法

    2019.4 - 2020.3   Full year

  • 人体構造と機能Ⅲ

    2019.4 - 2019.9   First semester

  • 生化学研究法

    2015.4 - 2016.3   Full year

  • 生化学実験法

    2015.4 - 2015.9   First semester

  • 生化学研究法

    2014.4 - 2015.3   Full year

  • 生化学実験法

    2014.4 - 2014.9   First semester

  • 生化学研究法

    2013.4 - 2014.3   Full year

  • 生化学実験法

    2013.4 - 2013.9   First semester

  • 生化学研究法

    2012.4 - 2013.3   Full year

  • 生化学実験法

    2012.4 - 2012.9   First semester

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Outline of Social Contribution and International Cooperation activities

  • 研究成果及び専門知識を分りやすく社会に情報提供、説明することにより社会との連携を推進する。
    国際学会及び国際シンポジウムへの積極的な参画。