Updated on 2024/10/01

Information

 

写真a

 
UCHIUMI TAKESHI
 
Organization
Faculty of Medical Sciences Department of Health Sciences Professor
School of Medicine Department of Health Sciences(Concurrent)
Graduate School of Medical Sciences Department of Health Sciences(Concurrent)
Title
Professor
Contact information
メールアドレス
Tel
0926426712
Profile
The Department of Health Science Laboratory Technology is working to develop the infrastructure of clinical chemistry and new measurement methods. In addition, mitochondrial research is being conducted as basic research. Mitochondrial DNA encodes the electron-transfer subunit that synthesizes ATP and is essential for cells to exist and function normally. Mitochondria are the largest site of reactive oxygen species generation in the cell, and mitochondrial DNA there is subject to very strong oxidative damage, and the concept that mitochondrial DNA mutations play an important role in the development and metastasis of diseases such as cancer, as well as in individual aging, is accepted. Functional analysis of genes involved in mitochondrial DNA replication, transcription, and translation is underway. Specifically, we are focusing on the mitochondrial ribosome-binding ERAL1 gene, which regulates translation, and the RNA-binding LRP130 gene. Functional analysis includes measurement of mitochondrial function (membrane potential, generation of reactive oxygen species, cell survival, and cell cycle) when gene expression is decreased by siRNA introduction, and functional evaluation (2) The CSD (cold shock domain) family of proteins has a nucleic acid binding domain consisting of approximately 78 amino acids that is highly conserved from E. coli to humans. CSD family proteins are ubiquitous proteins with a nucleic acid-binding domain consisting of about 78 amino acids that are highly conserved from E. coli to humans, and are expressed in many proliferating cells. In this study, (1) YB-1 knockout mice were generated and their functions were elucidated. Homozygous YB-1 knockout mice were embryonic lethal. (2) YB-1 was expressed almost throughout the mouse fetus by in situ hybridization. (3) YB-1 binds to the outer mitochondrial membrane and is involved in translation. (4) When YB-1 knockout fibroblasts were generated, cell proliferation was also decreased in experiments using YB-1 siRNA, confirming that the expression of EF-1 was upregulated upon YB-1 siRNA transfection. This suggests that YB-1 is deeply involved in cell proliferation, suggesting that YB-1 affects the expression of various proteins at the translational level. Furthermore, (5) YB1 was found to harbor a variety of microRNAs. This suggests that YB1 may be a potential molecular target in cancer, which could serve as a basis for new drug design. (6) Functional analysis of the mitochondrial matrix protein p32: From the individual level to the molecular level, we will examine how the mitochondria of intracellular organelles affect cells and individuals (7) Elucidation of new molecular mechanisms inferred from analysis of tissue-specific knockout mice of p32
External link

Degree

  • Doctor of Philosophy

  • Medical Doctor

Research History

  • Kyushu University Faculty of Medical Sciences Department of Health Sciences  Professor

    2007.6 - Present

  • 大分医科大学 1993年4月-1996年9月 九州大学院医医化学 1996年10月ー2005年3月 産業医科大学 分子生物学 2005年4月ー2007年5月

Research Interests・Research Keywords

  • Research theme:Physiological function analysis of mitochondrial protein ABCB10

    Keyword:mitochondria transporter

    Research period: 2022.6 - 2025.6

  • Research theme:New molecular mechnism of the functional interaction between mitochondria and lysosome

    Keyword:mitochondria, lysosome, NAD, functional interaction

    Research period: 2020.6 - 2026.6

  • Research theme:New approch for measurement of clinical chemistry

    Keyword:Clinical chemistry

    Research period: 2019.5 - 2022.5

  • Research theme:Mitochondria and cancer

    Keyword:mitochondria

    Research period: 2016.5

  • Research theme:Functional analysis of mitochondrial protein p32

    Keyword:p32 , mitochondria , translation

    Research period: 2010.5 - 2018.5

  • Research theme:Cross talk between cytoplasm and mitochndria translation

    Keyword:mitochondria translation

    Research period: 2008.5 - 2012.5

  • Research theme:Mitochondria related gene and Cancer

    Keyword:Mitochondria , tumor, translation, replication, siRNA

    Research period: 2007.6 - 2010.5

  • Research theme:Multifunction of YB-1 gene response to stress- cross-talk to translation, transcription and repair

    Keyword:YB-1, translation, transcription, repair

    Research period: 1998.4 - 2008.3

  • Research theme:ABCトランスポーター遺伝子群と疾病

    Keyword:ABCトランスポーター, 腫瘍、Dubin-Johnson症候群

    Research period: 1998.4 - 2004.3

Awards

  • 日本臨床検査医学会 優秀賞

    2012.10   日本臨床検査医学会   ミトコンドリアタンパクp32の病態機能解析

  • 上原記念生命科学研究奨励賞

    2003.3   上原記念生命科学財団   YB1の局所的翻訳調節機構

  • がん分子標的治療研究会奨励賞

    2002.6   がん分子標的治療研究会   抗がん剤感受性の分子標的としてのABCトランスポーター遺伝子群とYボックス結合蛋白

Papers

  • iMPAQT reveals that adequate mitohormesis from TFAM overexpression leads to life extension in mice Reviewed International journal

    K. Igami, H. Kittaka, M. Yagi, K. Gotoh, Y. Matsushima, T. Ide, M. Ikeda, S. Ueda, S. Nitta, M. Hayakawa, KI. Nakayama, M. Matsumoto, D. Kang, and T. Uchiumi

    Life Science Alliance   2024.7

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  • Cardiomyocyte-specific deletion of the mitochondrial transporter Abcb10 causes cardiac dysfunction via lysosomal-mediated ferroptosis

    Do, Y; Yagi, M; Hirai, H; Miki, K; Fukahori, Y; Setoyama, D; Yamamoto, M; Furukawa, T; Kunisaki, Y; Kang, DC; Uchiumi, T

    BIOSCIENCE REPORTS   44 ( 5 )   2024.5   ISSN:0144-8463 eISSN:1573-4935

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Bioscience reports  

    Heart function is highly dependent on mitochondria, which not only produce energy but also regulate many cellular functions. Therefore, mitochondria are important therapeutic targets in heart failure. Abcb10 is a member of the ABC transporter superfamily located in the inner mitochondrial membrane and plays an important role in haemoglobin synthesis, biliverdin transport, antioxidant stress, and stabilization of the iron transporter mitoferrin-1. However, the mechanisms underlying the impairment of mitochondrial transporters in the heart remain poorly understood. Here, we generated mice with cardiomyocyte-specific loss of Abcb10. The Abcb10 knockouts exhibited progressive worsening of cardiac fibrosis, increased cardiovascular risk markers and mitochondrial structural abnormalities, suggesting that the pathology of heart failure is related to mitochondrial dysfunction. As the mitochondrial dysfunction was observed early but mildly, other factors were considered. We then observed increased Hif1α expression, decreased NAD synthase expression, and reduced NAD+ levels, leading to lysosomal dysfunction. Analysis of ABCB10 knockdown HeLa cells revealed accumulation of Fe2+ and lipid peroxides in lysosomes, leading to ferroptosis. Lipid peroxidation was suppressed by treatment with iron chelators, suggesting that lysosomal iron accumulation is involved in ferroptosis. We also observed that Abcb10 knockout cardiomyocytes exhibited increased ROS production, iron accumulation, and lysosomal hypertrophy. Our findings suggest that Abcb10 is required for the maintenance of cardiac function and reveal a novel pathophysiology of chronic heart failure related to lysosomal function and ferroptosis.

    DOI: 10.1042/BSR20231992

    Web of Science

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  • Glucose starvation causes ferroptosis-mediated lysosomal dysfunction Reviewed International journal

    K. Miki, M.Yagi, D. Kang, Y. Kunisaki, K. Yoshimoto and T. Uchiumi

    iScience   2024.4

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  • Mitochondrial translation failure represses cholesterol gene expression via Pyk2-Gsk3β-Srebp2 axis Reviewed International journal

    2024.4

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  • Mitochondrial haplotype mutation alleviates respiratory defect of melas by restoring taurine modification in tRNA with 3243A > G mutation Reviewed International journal

    S. Ueda, M. Yagi, E. Tomoda, S. Matsumoto, Y. Ueyanagi, Y. Do, D. Setoyama, Y. Matsushima, A. Nagao, T. Suzuki, Y.Mori, N.Oyama, D. Kang, and T. Uchiumi

    Nucleic Acids Res   2023.7

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  • Improving lysosomal ferroptosis with NMN administration protects against heart failure Reviewed International journal

    M. Yagi, Y. Do, H. Hirai, K. Miki, T. Toshima, Y. Fukahori, D. Setoyama, C. Abe, YI. Nabeshima , D. Kang, and T. Uchiumi

    Life Sci Alliance   2023.6

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Induction of glioblastoma cell ferroptosis using combined treatment with chloramphenicol and 2-deoxy-D-glucose Reviewed International journal

    K. Miki, M.Yagi, N. Noguchi, Y. Do, R. Otuji, D. Kuga, D. Kang, Y. Yoshimoto and T. Uchiumi*

    Sci Rep   2023.6

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  • Mitochondrial translation deficiency impairs NAD+-mediated lysosomal acidification Invited Reviewed International journal

    Yagi, M., Toshima, T., Amamoto, R., Do, Y., Hirai, H., Setoyama, D., Kang, D. & Uchiumi, T.,

    EMBO Journal   2021.4

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  • Mutation and functional analysis of ABCC2/multidrug resistance protein 2 in a Japanese patient with Dubin-Johnson syndrome. Reviewed International journal

    Hepatol. Research   43   569 - 575   2013.2

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  • Characterization of 5'untranslated region of YB-1 mRNA and plausible regulation of translation. Reviewed International journal

    Fukuda, T., Ashizuka, M., Nakamura, T., Shibahara,K., Maeda, K., Izumi, I., Kohno, K., Kuwano, M.,and Uchiumi,T.,

    Nucleic Acid Res   2004.1

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  • The basic and clinical implications of ABC transporters, Y-box-binding protein-1 (YB-1) and angiogenesis-related factors in human malignancies. Invited Reviewed International journal

    Kuwano, M., Uchiumi, T., Hayakawa, H., Ono, M., Wada, M., Izumi, H., and Kohno, K

    Cancer Sci   94 ( 1 )   9 - 14   2003.1

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    DOI: 10.1111/j.1349-7006.2003.tb01344.x

  • The pleiotropic functions of the Y-box-binding protein, YB-1 Reviewed International journal

    Kohno K, Izumi H, Uchiumi T, Ashizuka M, and Kuwano M

    Bioessays   25 ( 7 )   691 - 698   2003.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/bies.10300

  • Novel translational control through an iron-responsive element by interaction of multifunctional protein YB-1 and IRP2. Reviewed International journal

    Ashizuka, M., Fukuda, T., Nakamura, T., Shirasuna, K., Iwai, K., Izumi, H., Kohno, K., Kuwano, M., and Uchiumi, T.

    Mol Cell Biol   22 ( 18 )   6375 - 6383   2002.1

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    DOI: 10.1128/MCB.22.18.6375-6383.2002

  • Trafficking and functional defects by mutations of the ATP-binding domains in MRP2 in patients with Dubin-Johnson syndrome. Reviewed International journal

    Hashimoto K.,Uchiumi T., Konno T., Ebihara T., Nakamura T., Wada M., Sakisaka S., Maniwa F., Amachi T., Ueda K., and Kuwano M.

    Hepatology   36 ( 5 )   1236 - 1245   2002.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1053/jhep.2002.36368

  • Involvement of the multidrug resistance protein 3 in drug sensitivity and its expression in human glioma. Reviewed International journal

    Haga, S., Hinoshita, E., Ikezaki, K., Fukui, M., Scheffer, G. L., Uchiumi, T. and Kuwano, M.

    Jpn. J. Cancer Res   92 ( 2 )   211 - 219   2001.1

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  • Enhanced expression of Human Multidrug resistance protein 3 by bile salt in human enterocytes: a transcriptional control of plausible bile acid transporter. Reviewed International journal

    Inokuchi, A., Hinoshita, E., Wada, M., Iwamoto, Y., Kohno, K., Kuwano, M. and Uchiumi, T.

    J. Biol. Chem.   276 ( 50 )   46822 - 46829   2001.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M104612200

  • Decreased expression of an ATP-binding cassette transporter, MRP2, in human livers with hepatitis C virus infection. Reviewed International journal

    Hinoshita, E., Taguchi, K., Inokuchi, A., Uchiumi, T., Kinukawa, N., Shimada, M., Tsuneyoshi, M., Sugimachi, K. and Kuwano, M.

    J. Hepatology   35 ( 6 )   765 - 773   2001.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0168-8278(01)00216-1

  • Increased expression of an ATP-binding cassette superfamily transporter, multidrug resistance protein 2, in human colorectal carcinomas. Reviewed International journal

    Hinoshita, E., Uchiumi, T., Taguchi, K., Kinukawa, N., Tsuneyoshi, M., Maehara, Y., Sugimachi, K. and Kuwano, M.

    Clin Cancer Res   6 ( 6 )   2401 - 2407   2000.1

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  • Enhanced transport of anticancer agents and leukotriene C4 by the human canalicular multispecific organic anion transporter (cMOAT/MRP2). Reviewed International journal

    Kawabe, T., Chen, Z., Wada, M., Uchiumi, T., Ono, M., Akiyama, S. and Kuwano, M.

    FEBS Lett.   456 ( 2 )   327 - 331   1999.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0014-5793(99)00979-5

  • The human multispecific resistance protein2 gene : functional characterization of 5'-flanking region and expression in hepatic cells. Reviewed International journal

    Tanaka,T., Uchiumi,T., Hinoshita,E., Inokuchi,A., Toh,S., Wada,M., Takano,H., Kohno,K., and Kuwano,M.

    Hepatology   1999.1

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  • Isolation of a novel human canalicular multispecific organic anion transporter, cMOAT2/MRP3, and its expression in cisplatin-resistant cancer cells with decreased ATP-dependent drug transport. Reviewed International journal

    Uchiumi, T., Hinoshita, E., Haga, S., Nakamura, T., Tanaka, T., Toh, S., Furukawa, M., Kawabe, T., Wada, M., Kagotani, K., Okumura, K., Kohno, K., Akiyama, S. and Kuwano, M.

    Biochem. Biophys. Res. Commun   252 ( 1 )   103 - 110   1998.1

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    DOI: 10.1006/bbrc.1998.9546

  • Cell cycle regulation of PLK promoter. Reviewed International journal

    Uchiumi, T., Longo, D. and Ferris, D

    J. Bio. Chem   1997.1

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  • AB029. Mitochondrial dysfunction and impaired growth of glioblastoma cell lines caused by antimicrobial agents inducing ferroptosis.

    Yoshimoto K, Miki K, Hatae R, Kuga D, Yagi M, Kunisaki Y, Uchiumi T

    Chinese clinical oncology   13 ( Suppl 1 )   AB029   2024.8   ISSN:2304-3865

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    DOI: 10.21037/cco-24-ab029

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  • Long-range and real-time PCR identification of a large <i>SERPINC1</i> deletion in a patient with antithrombin deficiency

    Matsumoto, S; Uchiumi, T; Ueyanagi, Y; Noda, N; Sakai, A; Hotta, T; Kato, K; Ohga, S; Kunisaki, Y; Kang, DC

    INTERNATIONAL JOURNAL OF HEMATOLOGY   120 ( 2 )   179 - 185   2024.8   ISSN:0925-5710 eISSN:1865-3774

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    Language:English   Publisher:International Journal of Hematology  

    Congenital antithrombin (AT) or serpin C1 deficiency, caused by a SERPINC1 abnormality, is a high-risk factor for venous thrombosis. SERPINC1 is prone to genetic rearrangement, because it contains numerous Alu elements. In this study, a Japanese patient who developed deep vein thrombosis during pregnancy and exhibited low AT activity underwent SERPINC1 gene analysis using routine methods: long-range polymerase chain reaction (PCR) and real-time PCR. Sequencing using long-range PCR products revealed no pathological variants in SERPINC1 exons or exon–intron junctions, and all the identified variants were homozygous, suggesting a deletion in one SERPINC1 allele. Copy number quantification for each SERPINC1 exon using real-time PCR revealed half the number of exon 1 and 2 copies compared with controls. Moreover, a deletion region was deduced by quantifying the 5′-upstream region copy number of SERPINC1 for each constant region. Direct long-range PCR sequencing with primers for the 5'-end of each presumed deletion region revealed a large Alu-mediated deletion (∼13 kb) involving SERPINC1 exons 1 and 2. Thus, a large deletion was identified in SERPINC1 using conventional PCR methods.

    DOI: 10.1007/s12185-024-03796-y

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  • Generation and maintenance of the circularized multimeric IS<i>26</i>-associated translocatable unit encoding multidrug resistance

    Aihara, M; Gotoh, Y; Shirahama, S; Matsushima, Y; Uchiumi, T; Kang, DC; Hayashi, T

    COMMUNICATIONS BIOLOGY   7 ( 1 )   597   2024.5   eISSN:2399-3642

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    Language:English   Publisher:Communications Biology  

    In gram-negative bacteria, IS26 often exists in multidrug resistance (MDR) regions, forming a pseudocompound transposon (PCTn) that can be tandemly amplified. It also generates a circular intermediate called the “translocatable unit (TU)”, but the TU has been detected only by PCR. Here, we demonstrate that in a Klebsiella pneumoniae MDR clone, mono- and multimeric forms of the TU were generated from the PCTn in a preexisting MDR plasmid where the inserted form of the TU was also tandemly amplified. The two modes of amplification were reproduced by culturing the original clone under antimicrobial selection pressure, and the amplified state was maintained in the absence of antibiotics. Mono- and multimeric forms of the circularized TU were generated in a RecA-dependent manner from the tandemly amplified TU, which can be generated in RecA-dependent and independent manners. These findings provide novel insights into the dynamic processes of genome amplification in bacteria.

    DOI: 10.1038/s42003-024-06312-4

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  • Vitamin K prophylaxis in neonates: comparing two different oral regimens

    Takahashi, D; Egami, N; Ochiai, M; Hotta, T; Suga, S; Ishimura, M; Kawaguchi, C; Uchiumi, T; Nishikubo, T; Nogami, K; Goto, K; Ohga, S

    JOURNAL OF PERINATOLOGY   2024.4   ISSN:0743-8346 eISSN:1476-5543

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    Language:English   Publisher:Journal of Perinatology  

    Objective: This prospective study compared PIVKA-II and PT-INR levels in infants who received two vitamin K (VK) prophylactic regimens. Methods: A single institution administered 119 healthy newborns 2 mg of VK syrup. Infants were assigned to a 3-time regimen (n = 56) with VK at birth, five days (5D), and 1-month-old (1 M), or a 13-time regimen (n = 63) with VK at birth, 5D, and then weekly for 11 weeks. Results: The 13-time regimen significantly lowered PIVKA-II and reduced PT-INR at 1 M in both breastfed (PIVKA-II: 18–16 mAU/mL, p = 0.02; PT-INR: 1.37–1.13, p < 0.01) and formula-fed infants (PIVKA-II: 18–15 mAU/mL, p = 0.01; PT-INR: 1.54–1.24, p < 0.01), compared to baseline measurements taken at 5D. The 3-time regimen did not significantly alter PIVKA-II levels and only improved PT-INR (2.00–1.50, p < 0.01) in formula-fed infants. Conclusion: The 13-time VK regimen significantly enhanced coagulation profiles more effectively than the 3-time regimen.

    DOI: 10.1038/s41372-024-01981-9

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  • Droplet digital polymerase chain reaction to measure heteroplasmic m.3243A&gt;G mitochondrial mutations

    Matsumoto, S; Uchiumi, T; Noda, N; Ueyanagi, Y; Hotta, T; Kang, DC

    LABORATORY MEDICINE   55 ( 2 )   227 - 233   2024.3   ISSN:0007-5027 eISSN:1943-7730

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    Language:English   Publisher:Lab Medicine  

    Objective: Different mitochondrial DNA genotypes can coexist in a cell population as well as in a single cell, a condition known as heteroplasmy. Here, we accurately determined the heteroplasmy levels of the m.3243A>G mutation, which is the most frequently identified mutation in patients with mitochondrial diseases, using droplet digital polymerase chain reaction (ddPCR). Methods: The m.3243A>G heteroplasmy levels in artificial heteroplasmy controls mixed with various proportions of wild-type and mutant plasmids were measured using ddPCR, PCR-restriction fragment length polymorphism, and Sanger sequencing. The m.3243A>G heteroplasmy levels in DNA, extracted from the peripheral blood of patients with suspected mitochondrial disease and healthy subjects, were determined using ddPCR. Results: The accuracy of the ddPCR method was high. The lower limit of detection was 0.1%, which indicated its higher sensitivity compared with other methods. The m.3243A>G heteroplasmy levels in peripheral blood, measured using ddPCR, correlated inversely with age at the time of analysis. The m.3243A>G mutation may be overlooked in the peripheral blood-derived DNA of elderly people, as patients >60 years of age have heteroplasmy levels <10%, which is difficult to detect using methods other than the highly sensitive ddPCR. Conclusion: ddPCR may be considered an accurate and sensitive method for measuring m.3243 A>G heteroplasmy levels of mitochondrial DNA.

    DOI: 10.1093/labmed/lmad063

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  • The clinical and genetic landscape of early-onset thrombophilia in Japan Reviewed International journal

    Naoki Egami, Masataka Ishimura, Masayuki Ochiai, Masako Ichiyama, Hirosuke Inoue, Souichi Suenobu, Toshiya Nishikubo, Keiji Nogami, Akira Ishiguro, Taeko Hotta, Takeshi Uchiumi, Dongchon Kang, Shouichi Ohga

    Pediatric Blood and Cancer   2024.3

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    DOI: 10.1002/pbc.30824

  • Pyrophosphate detection method using 5-Br-PAPS to detect nucleic acid amplification - Application to LAMP method Reviewed International journal

    Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa

    Analytical Biochemistr   2024.1

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    DOI: 10.1016/j.ab.2023.115371

  • 増大特集 代謝 Ⅰ.代謝と臓器・疾患 ミトコンドリア機能異常と老化機構

    内海 健

    生体の科学   74 ( 5 )   416 - 417   2023.10   ISSN:03709531 eISSN:18835503

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    Publisher:株式会社医学書院  

    DOI: 10.11477/mf.2425201731

    CiNii Research

  • Stroke in a protein C-deficient infant after stem cell transplant for CHARGE syndrome Invited Reviewed International journal

    Tanaka, Atsushi; Sakaguchi, Yoshiaki; Inoue, Hirosuke; Egami, Naoki; Sonoda, Yuri; Sonoda, Motoshi; Ishimura, Masataka; Ochiai, Masayuki; Hotta, Taeko; Uchiumi, Takeshi; Sakai, Yasunari; Ohga, Shouichi

    PEDIATRIC BLOOD & CANCER   70 ( 4 )   2023.4

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    DOI: 10.1002/pbc.30047

  • Monitoring of cell-free DNA with digital PCR in muscle invasive bladder cancer

    Tsukahara, S; Shiota, M; Uchiumi, T; Matsumoto, T; Nagakawa, S; Kodama, K; Eto, M

    CANCER SCIENCE   114   2189 - 2189   2023.2   ISSN:1347-9032 eISSN:1349-7006

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  • Mitochondrial and lysosomal crosstalk in aging diseases

    Yagi Mikako, Uchiumi Takeshi

    94 ( 6 )   882 - 887   2022.12   ISSN:00371017

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  • Mitochondrial dysfunction-induced high hCG associated with development of fetal growth restriction and pre-eclampsia with fetal growth restriction Reviewed International journal

    Kiyokoba R, Uchiumi T, Yagi M, Toshima T, Tsukahara S, Fujita Y, Kato K, Kang D.

    Scientific reports   2022.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41598-022-07893-y

  • Extracellular vesicles derived from GMSCs stimulated with TNF-α and IFN-α promote M2 macrophage polarization via enhanced CD73 and CD5L expression Reviewed International journal

    2022.12

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    Immunoregulatory properties of mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) are promising. Gingival tissue-derived MSCs (GMSCs) have unique immunoregulatory capacity and secrete large amounts of EVs. Recent findings suggest that priming MSCs with inflammatory stimuli is an effective strategy for cell-free therapy. However, the precise mechanism by which the contents of EVs are customized has not been fully elucidated. Here, we show that EVs derived from GMSCs primed with a combination of two pro-inflammatory cytokines, tumor necrosis factor-α (TNF-α) and interferon-α (IFN-α), synergistically promote anti-inflammatory M2 macrophage polarization by increasing the expression of cluster of differentiation 73 (CD73) and CD5 molecule-like (CD5L). Expression of CD73 by TNF-α/IFN-α stimulation was transcriptionally upregulated by the activation of mammalian target of rapamycin signaling and nuclear translocation of hypoxia-inducible factor 1α in GMSCs. TNF-α/IFN-α treatment also significantly increased the expression of CD5L mRNA via the transcription factor DNA-binding protein inhibitor ID3 and liver X receptor. Interestingly, exosomal CD5L is a prerequisite for the synergistic effect of EVs-mediated M2 macrophage polarization. These results indicate that combined pre-licensing with TNF-α and IFN-α in GMSCs is ideal for enhancing the anti-inflammatory function of EVs, which contributes to the establishment of a therapeutic tool.

    DOI: 10.1038/s41598-022-17692-0

  • miR-1260b inhibits periodontal bone loss by targeting ATF6 beta mediated regulation of ER stress Invited Reviewed International journal

    Hayashi, Chikako; Fukuda, Takao; Kawakami, Kentaro; Toyoda, Masaaki; Nakao, Yuki; Watanabe, Yukari; Shinjo, Takanori; Sano, Tomomi; Iwashita, Misaki; Yotsumoto, Karen; Shida, Miyu; Taketomi, Takaharu; Sanui, Terukazu; Uchiumi, Takeshi; Kanematsu, Takashi; Nishimura, Fusanori

    FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY   10   2022.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3389/icell.2022.1061216

  • Mitochondrial dysfunction and impaired growth of glioblastoma cell lines caused by antimicrobial agents inducing ferroptosis under glucose starvation Invited Reviewed International journal

    Miki, Kenji; Yagi, Mikako; Yoshimoto, Koji; Kang, Dongchon; Uchiumi, Takeshi

    ONCOGENESIS   11 ( 1 )   2022.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41389-022-00437-z

  • Extracellular vesicles expressing CEACAM proteins in the urine of bladder cancer patients Invited Reviewed International journal

    Igami, Ko; Uchiumi, Takeshi; Shiota, Masaki; Ueda, Saori; Tsukahara, Shigehiro; Akimoto, Masaru; Eto, Masatoshi; Kang, Dongchon

    CANCER SCIENCE   113 ( 9 )   3120 - 3133   2022.9

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    DOI: 10.1111/cas.15438

  • TFAM expression in brown adipocytes confers obesity resistance by secreting extracellular vesicles that promote self-activation Invited Reviewed International journal

    Fujii, Masakazu; Setoyama, Daiki; Gotoh, Kazuhito; Dozono, Yushi; Yagi, Mikako; Ikeda, Masataka; Ide, Tomomi; Uchiumi, Takeshi; Kang, Dongchon

    ISCIENCE   25 ( 9 )   2022.9

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    DOI: 10.1016/j.isci.2022.104889

  • Cancer genomic profiling identified dihydropyrimidine dehydrogenase deficiency in bladder cancer promotes sensitivity to gemcitabine Invited Reviewed International journal

    Tsukahara, Shigehiro; Shiota, Masaki; Takamatsu, Dai; Nagakawa, Shohei; Matsumoto, Takashi; Kiyokoba, Ryo; Yagi, Mikako; Setoyama, Daiki; Noda, Nozomi; Matsumoto, Shinya; Hayashi, Tetsutaro; Contreras-Sanz, Alberto; Black, Peter C.; Inokuchi, Junichi; Kohashi, Kenichi; Oda, Yoshinao; Uchiumi, Takeshi; Eto, Masatoshi; Kang, Dongchon

    SCIENTIFIC REPORTS   12 ( 1 )   2022.5

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    DOI: 10.1038/s41598-022-12528-3

  • Clinical impact of HSD3B1 polymorphism by metastatic volume and somatic HSD3B1 alterations in advanced prostate cancer Reviewed International journal

    Masaki Shiota, Naohiro Fujimoto, Yohei Sekino, Shigehiro Tsukahara, Shohei Nagakawa, Dai Takamatsu, Tatsuro Abe, Fumio Kinoshita, Shohei Ueda, Miho Ushijima, Takashi Matsumoto, Eiji Kashiwagi, Junichi Inokuchi, Takeshi Uchiumi, Yoshinao Oda, Masatoshi Eto

    Andrologia   2022.2

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    DOI: 10.1111/and.14307

  • A genome-wide association study on intravesical recurrence after BCG therapy for non-muscle invasive bladder cancer

    Nagakawa, S; Shiota, M; Fujimoto, N; Yamamoto, Y; Tsukahara, S; Matsumoto, T; Kashiwagi, E; Takeuchi, A; Inokuchi, J; Uchiumi, T; Matsuyama, H; Eto, M

    CANCER SCIENCE   113   1588 - 1588   2022.2   ISSN:1347-9032 eISSN:1349-7006

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  • Resistance to chemotherapy with gemcitabine associated with hyperexpression of DPYD in muscle invasive bladder cancers

    Tsukahara, S; Shiota, M; Uchiumi, T; Matsumoto, T; Hayashi, T; Nagakawa, S; Eto, M

    CANCER SCIENCE   113   1726 - 1726   2022.2   ISSN:1347-9032 eISSN:1349-7006

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  • HSD3B1 polymorphism and genomic HSD3B1 aberrations in prostate cancer

    Shiota, M; Fujimoto, N; Sekino, Y; Tsukahara, S; Nagakawa, S; Takamatsu, D; Abe, T; Kinoshita, F; Ueda, S; Matsumoto, T; Kashiwagi, E; Takeuchi, A; Inokuchi, J; Uchiumi, T; Oda, Y; Eto, M

    CANCER SCIENCE   113   1591 - 1591   2022.2   ISSN:1347-9032 eISSN:1349-7006

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  • Clinical Impact of Heritable Thrombophilia on Neonatal-Onset Thromboembolism: A Nationwide Study in Japan Invited Reviewed International journal

    Naoki Egami MD 1, Masayuki Ochiai MD, PhD 1, Masako Ichiyama MD, PhD 2, Hirosuke Inoue MD, PhD 1, Motoshi Sonoda MD 1, Masataka Ishimura MD, PhD 1, Souichi Suenobu MD, PhD 3, Toshiya Nishikubo MD, PhD 4, Akira Ishiguro MD, PhD 5, Taeko Hotta MT 6, Takeshi Uchiumi MD, PhD 6, Dongchon Kang MD, PhD 6, Shouichi Ohga MD, PhD 1

    JOURNAL OF PEDIATRICS   238   259 - +   2021.11

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    DOI: 10.1016/j.jpeds.2021.07.001

  • Stepwise Evolution of a Klebsiella pneumoniae Clone within a Host Leading to Increased Multidrug Resistance Reviewed International journal

    Mai Yoshino, Masamune Aihara, Yasuhiro Gotoh, Masaru Akimoto, Wakana Tatsuhara, Makiko Kiyosuke, Yuichi Matsushima, Takeshi Uchiumi, Tetsuya Hayashi, Dongchon Kang

    mSphere   2021.11

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    DOI: 10.1128/mSphere.00734-21

  • The impact of single-nucleotide polymorphisms on intravesical recurrence after bacillus Calmette–Guérin therapy for non-muscle invasive bladder cancer in a genome-wide association study Reviewed International journal

    2021.10

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    DOI: 10.1016/j.urolonc.2021.05.034

  • Mitochondrial Lon protease is a gatekeeper for proteins newly imported into the matrix Reviewed International journal

    Yuichi Matsushima, Kazuya Takahashi, Song Yue, Yuki Fujiyoshi, Hideaki Yoshioka, Masamune Aihara, Daiki Setoyama, Takeshi Uchiumi, Satoshi Fukuchi, Dongchon Kang

    Communications Biology   2021.10

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    DOI: 10.1038/s42003-021-02498-z

  • Development of a protein assay with copper chelator chromeazurol B, based on the biuret reaction Reviewed International journal

    Eisaku Hokazono, Eri Ota, Taiki Goto, Saori Fukumoto, Yuzo Kayamori, Takeshi Uchiumi, Susumu Osawa

    Analytical Biochemistry   2021.10

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    DOI: 10.1016/j.ab.2021.114320

  • Differential Impact of TGFB1 Variation by Metastatic Status in Androgen-Deprivation Therapy for Prostate Cancer Reviewed International journal

    Masaki Shiota, Naohiro Fujimoto, Takashi Matsumoto, Shigehiro Tsukahara, Shohei Nagakawa, Shohei Ueda, Miho Ushijima, Eiji Kashiwagi, ario takeuchi, Junichi Inokuchi, Takeshi Uchiumi, Masatoshi Eto

    Frontiers in Oncology   2021.5

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    DOI: 10.3389/fonc.2021.697955

  • Exosomes from TNF-α-treated human gingiva-derived MSCs enhance M2 macrophage polarization and inhibit periodontal bone loss Reviewed International journal

    2021.3

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  • Genomic characteristics revealed by targeted exon sequencing of testicular germ cell tumors in Japanese men Invited Reviewed International journal

    Matsumoto, T., Shiota, M., Uchiumi, T., Ueda, S., Tsukahara, S., Toshima, T., Matsumoto, S., Noda, N., Eto, M. & Kang, D., 1 2021, In: International Journal of Urology. 28, 1, p. 40-46 7 p.

    International Journal of Urology   2021.2

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  • Within-host evolution of a Klebsiella pneumoniae clone: Selected mutations associated with the alteration of outer membrane protein expression conferred multidrug resistance Reviewed International journal

    Masamune Aihara, Ruriko Nishida, Masaru Akimoto, Yasuhiro Gotoh, Makiko Kiyosuke, Takeshi Uchiumi, Mitsuaki Nishioka, Yuichi Matsushima, Tetsuya Hayashi, Dongchon Kang

    Journal of Antimicrobial Chemotherapy   2021.2

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    DOI: 10.1093/jac/dkaa439

  • Gene amplification of YB-1 in castration-resistant prostate cancer in association with aberrant androgen receptor expression Reviewed International journal

    Masaki Shiota, Yohei Sekino, Shigehiro Tsukahara, Tatsuro Abe, Fumio Kinoshita, Kenjiro Imada, Shohei Ueda, Miho Ushijima, Shohei Nagakawa, Takashi Matsumoto, Eiji Kashiwagi, ario takeuchi, Junichi Inokuchi, Takeshi Uchiumi, Yoshinao Oda, Masatoshi Eto

    Cancer Science   2021.1

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    DOI: 10.1111/cas.14695

  • The association between missense polymorphisms in SRD5A2 and HSD3B1 and treatment failure with abiraterone for castration-resistant prostate cancer Invited Reviewed International journal

    Shiota, M., Akamatsu, S., Narita, S., Sumiyoshi, T., Fujiwara, M., Uchiumi, T., Ogawa, O., Habuchi, T. & Eto, M.,

    Pharmacogenomics Journal   2021.1

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  • Polymorphisms in androgen metabolism genes with serum testosterone levels and prognosis in androgen-deprivation therapy Reviewed International journal

    Masaki Shiota, Satoshi Endo, Naohiro Fujimoto, Shigehiro Tsukahara, Miho Ushijima, Eiji Kashiwagi, ario takeuchi, Junichi Inokuchi, Takeshi Uchiumi, Masatoshi Eto

    Urologic Oncology: Seminars and Original Investigations   2020.11

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    DOI: 10.1016/j.urolonc.2020.06.033

  • Mitochondrial translation inhibition triggers ATF4 activation, leading to integrated stress response but not to mitochondrial unfolded protein response Reviewed International journal

    Katsuhiko Sasaki, Takeshi Uchiumi, Takahiro Toshima, Mikako Yagi, Yura Do, Haruka Hirai, Ko Igami, Kazuhito Gotoh, Dongchon Kang

    Bioscience reports   2020.11

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    DOI: 10.1042/BSR20201289

  • Mitochondrial Protein Synthesis Is Essential for Terminal Differentiation of CD45– TER119–Erythroid and Lymphoid Progenitors Reviewed International journal

    2020.11

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    DOI: 10.1016/j.isci.2020.101654

  • High-throughput screen identifies 5-HT receptor as a modulator of AR and a therapeutic target for prostate cancer Reviewed International journal

    Momoe Itsumi, Masaki Shiota, Yohei Sekino, Miho Ushijima, Eiji Kashiwagi, Ario Takeuchi, Junichi Inokuchi, Shunichi Kajioka, Takeshi Uchiumi, Masatoshi Eto

    Prostate   2020.8

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    DOI: 10.1002/pros.24022

  • Mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke-like episodes due to m.3243A > G mutation in a 76-year-old woman Reviewed

    Kana Ueki, Yoshinobu Wakisaka, Kuniyuki Nakamura, Yuji Shono, Shinichi Wada, Yoji Yoshikawa, Yuta Matsukuma, Takeshi Uchiumi, Dongchong Kang, Takanari Kitazono, Tetsuro Ago

    Journal of the Neurological Sciences   412   2020.5

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    DOI: 10.1016/j.jns.2020.116791

  • Genome-wide association study of genetic variations associated with treatment failure after intravesical bacillus Calmette–Guérin therapy for non-muscle invasive bladder cancer Reviewed

    2020.1

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    Bacillus Calmette–Guérin (BCG) instillation is a key therapy to manage non-muscle invasive bladder cancer (NMIBC). However, intravesical BCG therapy fails in approximately half of the patients, leading to recurrence and progression. We aimed to reveal the genetic variations associated with treatment failure after intravesical BCG therapy for NMIBC. This study included 91 Japanese patients treated with BCG instillation for NMIBC. Genomic DNA was obtained from patient whole-blood samples, and a genome-wide association study and genotyping for target regions were performed. The association between genetic variation and treatment failure was analyzed by genome-wide association in 44 patients as the discovery cohort. As a validation study, candidate single nucleotide polymorphisms (SNPs) were examined among 47 patients in another cohort. The genome-wide association study indicated 19 candidate SNPs (rs1607282, rs7825442, rs1319325, rs3738088, rs4250, rs11894207, rs161448, rs2764326, rs2814707, rs3787194, rs58081719, rs3095966, rs73520681, rs16877113, rs16887173, rs10269584, rs11772249, rs118137814, and rs61094339) associated with BCG failure. Following the cumulative analysis of candidate SNPs, 2-gene (rs73520681 and rs61094339) and 4-gene (rs4250, rs11894207, rs73520681, and rs61094339) models successfully predicted treatment failure after intravesical BCG therapy. This study showed that several SNPs were possibly associated with outcome after intravesical BCG therapy in a Japanese population with NMIBC. The cumulative models of these SNPs may have value in clinical applications, although this should be confirmed in future studies.

    DOI: 10.1007/s00262-020-02533-8

  • Impaired plasmalogen synthesis dysregulates liver X receptor-dependent transcription in cerebellum Reviewed

    Masanori Honsho, Fabian Dorninger, Yuichi Abe, Daiki Setoyama, Ryohei Ohgi, Takeshi Uchiumi, Dongchon Kang, Johannes Berger, Yukio Fujiki

    Journal of Infectious Diseases   220 ( 4 )   353 - 361   2019.8

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    DOI: 10.1093/jb/mvz043

  • Toxic myopathy with multiple deletions in mitochondrial DNA associated with long-term use of oral anti-viral drugs for hepatitis B: A case study Invited Reviewed International journal

    Fujii, T., Takase, K. I., Honda, H., Kawamura, N., Yamasaki, R., Urata, M., Uchiumi, T., Iwaki, T. & Kira, J-I

    NEUROPATHOLOGY   39 ( 2 )   162 - 167   2019.4

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/neup.12548

  • Diagnostic challenge of the newborn patients with heritable protein C deficiency Reviewed

    Masako Ichiyama, Hirosuke Inoue, Masayuki Ochiai, masataka ishimura, Akira Shiraishi, Junko Fujiyoshi, Hironori Yamashita, Kazuo Sato, Shinya Matsumoto, Taeko Hotta, Takeshi Uchiumi, Dongchon Kang, Shoichi Ohga

    Journal of Perinatology   39 ( 2 )   212 - 219   2019.2

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    DOI: 10.1038/s41372-018-0262-0

  • Association of Missense Polymorphism in HSD3B1 With Outcomes Among Men With Prostate Cancer Treated With Androgen-Deprivation Therapy or Abiraterone Reviewed

    Masaki Shiota, Shintaro Narita, Shusuke Akamatsu, Naohiro Fujimoto, Takayuki Sumiyoshi, Maki Fujiwara, Takeshi Uchiumi, Tomonori Habuchi, Osamu Ogawa, Masatoshi Eto

    JAMA network open   2 ( 2 )   e190115   2019.2

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    DOI: 10.1001/jamanetworkopen.2019.0115

  • Genetic Polymorphism in Sex Hormone-binding Globulin With a Prognosis of Androgen Deprivation Therapy in Metastatic Prostate Cancer Among Japanese Men Reviewed

    masaki shiota, Naohiro Fujimoto, Shigehiro Tsukahara, Miho Ushijima, ario takeuchi, Eiji Kashiwagi, Junichi Inokuchi, Katsunori Tatsugami, Takeshi Uchiumi, Masatoshi Eto

    Clinical Genitourinary Cancer   2019.1

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    DOI: 10.1016/j.clgc.2019.03.021

  • The impact of genetic polymorphism on CYP19A1 in androgen-deprivation therapy among Japanese men Reviewed

    masaki shiota, Naohiro Fujimoto, Shigehiro Tsukahara, Miho Ushijima, ario takeuchi, Eiji Kashiwagi, Junichi Inokuchi, Katsunori Tatsugami, Takeshi Uchiumi, Masatoshi Eto

    Cancer chemotherapy and pharmacology   2019.1

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    DOI: 10.1007/s00280-019-03811-8

  • Mitochondrial p32/C1qbp Is a Critical Regulator of Dendritic Cell Metabolism and Maturation Reviewed International journal

    Gotoh K, Morisaki T, Setoyama D, Sasaki K, Yagi M, Igami K, Mizuguchi S, Uchiumi T, Fukui Y and Kang D

    CELL REPORTS   25 ( 7 )   1800 - +   2018.11

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    DOI: 10.1016/j.celrep.2018.10.057

  • Collateral resistance to taxanes in enzalutamide-resistant prostate cancer through aberrant androgen receptor and its variants Reviewed

    masaki shiota, Takashi Dejima, Yoshiaki Yamamoto, ario takeuchi, Kenjiro Imada, Eiji Kashiwagi, Junichi Inokuchi, Katsunori Tatsugami, Shunichi Kajioka, Takeshi Uchiumi, Masatoshi Eto

    Cancer Science   109 ( 10 )   3224 - 3234   2018.10

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    DOI: 10.1111/cas.13751

  • Purpura fulminans in congenital protein C deficiency successfully treated with fresh frozen plasma and thrombomodulin Reviewed

    Takuma Hayami, Akihiko Yamaguchi, Takeshi Kato, Toshihiro Tanaka, Yuka Nishizawa, Takahide Yanagi, Takashi Taga, Shinya Matsumoto, Takeshi Uchiumi, Noriki Fujimoto

    Journal of Dermatology   45 ( 6 )   e165 - e166   2018.6

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    DOI: 10.1111/1346-8138.14194

  • Mineralocorticoid receptor signaling affects therapeutic effect of enzalutamide Reviewed

    masaki shiota, Naohiro Fujimoto, Katuyoshi Higashijima, Kenjiro Imada, Eiji Kashiwagi, ario takeuchi, Junichi Inokuchi, Katsunori Tatsugami, Shunichi Kajioka, Takeshi Uchiumi, Masatoshi Eto

    Prostate   2018.1

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    DOI: 10.1002/pros.23661

  • Prognostic impact of genetic polymorphism in mineralocorticoid receptor and comorbidity with hypertension in androgen-deprivation therapy Reviewed

    masaki shiota, Naohiro Fujimoto, Kenjiro Imada, Eiji Kashiwagi, ario takeuchi, Junichi Inokuchi, Katsunori Tatsugami, Shunichi Kajioka, Takeshi Uchiumi, Masatoshi Eto

    Frontiers in Oncology   8 ( DEC )   2018.1

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    DOI: 10.3389/fonc.2018.00635

  • The Association of Polymorphisms in the Gene Encoding Gonadotropin-Releasing Hormone with Serum Testosterone Level during Androgen Deprivation Therapy and Prognosis of Metastatic Prostate Cancer Reviewed

    masaki shiota, Naohiro Fujimoto, ario takeuchi, Eiji Kashiwagi, Takashi Dejima, Junichi Inokuchi, Katsunori Tatsugami, Akira Yokomizo, Shunichi Kajioka, Takeshi Uchiumi, Masatoshi Eto

    Journal of Urology   2018.1

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    DOI: 10.1016/j.juro.2017.09.076

  • Neural-specific deletion of mitochondrial p32/C1qbp leads to leukoencephalopathy due to undifferentiated oligodendrocyte and axon degeneration Reviewed

    Mikako Yagi, Takeshi Uchiumi, Noriaki Sagata, Daiki Setoyama, Rie Amamoto, Yuichi Matsushima, Dongchon Kang

    Scientific Reports   7 ( 1 )   2017.12

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    DOI: 10.1038/s41598-017-15414-5

  • Isolated and repeated stroke-like episodes in a middle-aged man with a mitochondrial ND3 T10158C mutation A case report Reviewed

    Satomi Mezuki, Kenji Fukuda, Tomonaga Matsushita, Yoshihisa Fukushima, ryu matsuo, Yu ichi Goto, Takehiro Yasukawa, Takeshi Uchiumi, Dongchon Kang, Takanari Kitazono, Tetsuro Ago

    BMC Neurology   17 ( 1 )   2017.12

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    DOI: 10.1186/s12883-017-1001-4

  • Drosophila protease ClpXP specifically degrades DmLRPPRC1 controlling mitochondrial mRNA and translation Reviewed

    Yuichi Matsushima, Yuta Hirofuji, Masamune Aihara, Song Yue, Takeshi Uchiumi, Laurie S. Kaguni, Dongchon Kang

    Scientific Reports   7 ( 1 )   2017.12

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    DOI: 10.1038/s41598-017-08088-6

  • Doxycycline induces apoptosis via ER stress selectively to cells with a cancer stem cell-like properties Importance of stem cell plasticity Reviewed

    Takashi Matsumoto, Takeshi Uchiumi, Keisuke Monji, Mikako Yagi, Daiki Setoyama, Rie Amamoto, Yuichi Matsushima, masaki shiota, Masatoshi Eto, Dongchon Kang

    Oncogenesis   6 ( 11 )   2017.11

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    DOI: 10.1038/s41389-017-0009-3

  • Amelogenin induces M2 macrophage polarisation via PGE2/cAMP signalling pathway Reviewed

    Kensuke Yamamichi, Takao Fukuda, Terukazu Sanui, Kyousuke Toyoda, Urara Tanaka, Yuki Nakao, Karen Yotsumoto, Hiroaki Yamato, Takaharu Taketomi, Takeshi Uchiumi, Fusanori Nishimura

    Archives of Oral Biology   83   241 - 251   2017.11

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    DOI: 10.1016/j.archoralbio.2017.08.005

  • Biallelic C1QBP Mutations Cause Severe Neonatal-, Childhood-, or Later-Onset Cardiomyopathy Associated with Combined Respiratory-Chain Deficiencies Reviewed

    American Journal of Human Genetics   101 ( 4 )   525 - 538   2017.10

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    DOI: 10.1016/j.ajhg.2017.08.015

  • Cardiomyocyte-specific loss of mitochondrial p32/C1qbp causes cardiomyopathy and activates stress responses Reviewed

    Toshiro Saito, Takeshi Uchiumi, Mikako Yagi, Rie Amamoto, Daiki Setoyama, Yuichi Matsushima, Dongchon Kang

    Cardiovascular Research   113 ( 10 )   1173 - 1185   2017.8

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    DOI: 10.1093/cvr/cvx095

  • The clinical presentation and genotype of protein C deficiency with double mutations of the protein C gene Reviewed

    Hirofumi Inoue, Shin Ichi Terachi, Takeshi Uchiumi, Tetsuji Sato, Michiyo Urata, masataka ishimura, Yui Koga, Taeko Hotta, Toshiro Hara, Dongchon Kang, Shoichi Ohga

    Pediatric Blood and Cancer   64 ( 7 )   2017.7

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    DOI: 10.1002/pbc.26404

  • p32 is Required for Appropriate Interleukin-6 Production Upon LPS Stimulation and Protects Mice from Endotoxin Shock Reviewed

    Katsuhiko Sasaki, Kazuhito Gotou, Sho Miake, Daiki Setoyama, Mikako Yagi, Ko Igami, Takeshi Uchiumi, Dongchon Kang

    EBioMedicine   20   161 - 172   2017.6

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    DOI: 10.1016/j.ebiom.2017.05.018

  • Protein kinase C regulates Twist1 expression via NF-κB in prostate cancer Reviewed

    24 ( 4 )   171 - 180   2017.4

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    The progression of prostate cancer to metastatic and castration-resistant disease represents a critical step. We previously showed that protein kinase C (PKC) activation followed by Twist1 and androgen receptor (AR) induction played a critical role in castration resistance, but the precise molecular mechanism remains unknown. This study aimed to elucidate the relevant molecular mechanism, focusing on NF-κB transcription factor. We examined the activity of NF-κB after PKC inhibition, and the expression of Twist1 and AR after inhibition of NF-κB in human prostate cancer cells. We also investigated the status of PKC/NF-κB after inhibition of AR signaling in cells resistant to hormonal therapy. As a result, inhibition of PKC signaling using knockdown and smallmolecule inhibition of PKC suppressed RelA activity, while blocking NF-κB suppressed Twist1 and AR expression. Conversely, inhibition of AR signaling by androgen depletion and the novel antiandrogen enzalutamide induced PKC and RelA activation, resulting in Twist1/AR induction at the transcript level. Moreover, inhibition of NF-κB signaling prevented enzalutamide-induced Twist1 and AR induction. Finally, NF-κB was activated in both castration-resistant and enzalutamide-resistant cells. In conclusion, NF-κB signaling was responsible for Twist1 upregulation by PKC in response to AR inhibition, resulting in aberrant activation of AR. NF-κB signaling thus appears to play a critical role in promoting both castration resistance and enzalutamide resistance in PKC/Twist1 signaling in prostate cancer.

    DOI: 10.1530/ERC-16-0384

  • A case of rhabdomyolysis after status epilepticus without stroke-like episodes in mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke-like episodes Reviewed

    Jun Yokoyama, hiroo yamaguchi, Hiroshi Shigeto, Takeshi Uchiumi, Hiroyuki Murai, Jun-Ichi Kira

    Clinical Neurology   57 ( 7 )   400 - 401   2017.1

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    DOI: 10.5692/clinicalneurol.cn-001044

  • Mitochondrial nucleic acid binding proteins associated with diseases Reviewed

    Takeshi Uchiumi, Dongchon Kang

    Frontiers in Bioscience - Landmark   22   168 - 179   2017.1

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    DOI: 10.2741/4479

  • Gene polymorphisms in antioxidant enzymes correlate with the efficacy of androgen-deprivation therapy for prostate cancer with implications of oxidative stress Reviewed

    masaki shiota, N. Fujimoto, M. Itsumi, ario takeuchi, Junichi Inokuchi, Katsunori Tatsugami, A. Yokomizo, Shunichi Kajioka, Takeshi Uchiumi, Masatoshi Eto

    Annals of Oncology   28 ( 3 )   569 - 575   2017.1

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    DOI: 10.1093/annonc/mdw646

  • Comparison of the Abbott RealTime HCV and Roche COBAS Ampliprep/COBAS TaqMan HCV assays for the monitoring of sofosbuvir-based therapy Reviewed

    Eiichi Ogawa, Norihiro Furusyo, Murata Masayuki, Motohiro Shimizu, Kazuhiro Toyoda, Taeko Hotta, Takeshi Uchiumi, Jun Hayashi

    Antiviral Therapy   22 ( 1 )   61 - 70   2017.1

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    DOI: 10.3851/IMP3085

  • Melanin pigment interrupts the fluorescence staining of mitochondria in melanocytes Reviewed

    Hideya Ando, Yoshiaki Ohagi, Moemi Yoshida, Satoshi Yoshimoto, Yusuke Higashi, Masayuki Yagi, Keisuke Monji, Mikako Yagi, Takeshi Uchiumi, Dongchon Kang, Masamitsu Ichihashi

    Journal of Dermatological Science   84 ( 3 )   349 - 351   2016.12

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    DOI: 10.1016/j.jdermsci.2016.08.533

  • The Expression of Ubiquitous Mitochondrial Creatine Kinase Is Downregulated as Prostate Cancer Progression Invited Reviewed International journal

    Rie Amamoto, Takeshi Uchiumi, Mikako Yagi, Keisuke Monji, Song, Yoshinao Oda, Shiota M, Akira Yokomizo, Seiji Naito, Dongchon Kang

    JOURNAL OF CANCER   7 ( 1 )   50 - 59   2016.7

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    DOI: 10.7150/jca.13207

  • Equol inhibits prostate cancer growth through degradation of androgen receptor by S-phase kinase-associated protein 2 Reviewed

    Momoe Itsumi, masaki shiota, ario takeuchi, Eiji Kashiwagi, Junichi Inokuchi, Katsunori Tatsugami, Shunichi Kajioka, Takeshi Uchiumi, Seiji Naito, Masatoshi Eto, Akira Yokomizo

    Cancer Science   107 ( 7 )   1022 - 1028   2016.7

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    DOI: 10.1111/cas.12948

  • The Expression of Ubiquitous Mitochondrial Creatine Kinase Is Downregulated as Prostate Cancer Progression. Reviewed International journal

    Amamoto R, Uchiumi T, Yagi M, Monji K, Song Y, Oda Y,

    J Cancer   7   50 - 59   2016.7

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    DOI: 10.7150/jca.13207

  • Dihydroorotate dehydrogenase depletion hampers mitochondrial function and osteogenic differentiation in osteoblasts Reviewed

    Jingxian Fang, Haruyoshi Yamaza, Takeshi Uchiumi, Yoshihiro Hoshino, Keiji Masuda, Yuta Hirofuji, Frank A.D.T.G. Wagener, Dongchon Kang, Kazuaki Nonaka

    European Journal of Oral Sciences   124 ( 3 )   241 - 245   2016.6

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    DOI: 10.1111/eos.12270

  • Neuraminidase Amino Acid Sequences of Influenza A/H3N2 and B Viruses Isolated from Influenza Patients in the 2014/15 Japanese Influenza Season Reviewed

    Hideyuki Ikematsu, Yong Jeong, Kenjiro Shirane, Hidehiro Toh, Hiroyuki Sasaki, Shinya Matsumoto, Nozomi Noda, Taeko Hotta, Takeshi Uchiumi, Dongchon Kang

    Fukuoka Acta Medica   107 ( 5 )   98 - 104   2016.5

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  • Impact of HCV kinetics on treatment outcome differs by the type of real-time HCV assay in NS3/4A protease inhibitor-based triple therapy Reviewed

    Eiichi Ogawa, Norihiro Furusyo, Murata Masayuki, Takeo Hayashi, Motohiro Shimizu, Haru Mukae, Kazuhiro Toyoda, Taeko Hotta, Takeshi Uchiumi, Jun Hayashi

    Antiviral Research   126   35 - 42   2016.2

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    DOI: 10.1016/j.antiviral.2015.12.001

  • A case of rhabdomyolysis after status epilepticus without stroke-like episodes in mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke-like episodes Reviewed

    Jun Yokoyama, hiroo yamaguchi, Hiroshi Shigeto, Takeshi Uchiumi, Hiroyuki Murai, Jun-Ichi Kira

    Clinical Neurology   56 ( 3 )   204 - 207   2016.1

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    DOI: 10.5692/clinicalneurol.cn-000834

  • Serum depletion induced cancer stem cell-like phenotype due to nitric oxide synthesis in oncogenic HRas transformed cells Reviewed

    Keisuke Monji, Takeshi Uchiumi, Saki Hoshizawa, Mikako Yagi, Takashi Matsumoto, Daiki Setoyama, Yuichi Matsushima, Kazuhito Gotou, Rie Amamoto, Dongchon Kang

    Oncotarget   7 ( 46 )   75221 - 75234   2016.1

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    DOI: 10.18632/oncotarget.12117

  • Potential role for YB-1 in castration-resistant prostate cancer and resistance to enzalutamide through the androgen receptor V7 Reviewed

    masaki shiota, Naohiro Fujimoto, Kenjiro Imada, Akira Yokomizo, Momoe Itsumi, ario takeuchi, Hidetoshi Kuruma, Junichi Inokuchi, Katsunori Tatsugami, Takeshi Uchiumi, Yoshinao Oda, Seiji Naito

    Journal of the National Cancer Institute   108 ( 7 )   2016.1

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    DOI: 10.1093/jnci/djw005

  • Grp78 Is Critical for Amelogenin-Induced Cell Migration in a Multipotent Clonal Human Periodontal Ligament Cell Line Reviewed

    Kyousuke Toyoda, Takao Fukuda, Terukazu Sanui, Urara Tanaka, Kensuke Yamamichi, Ryo Atomura, Hidefumi Maeda, Atsushi Tomokiyo, Takaharu Taketomi, Takeshi Uchiumi, Fusanori Nishimura

    Journal of Cellular Physiology   231 ( 2 )   414 - 427   2016.1

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    DOI: 10.1002/jcp.25087

  • Crosstalk between epithelial-mesenchymal transition and castration resistance mediated by Twist1/AR signaling in prostate cancer Invited Reviewed International journal

    Shiota M, Akira Yokomizo, Takeshi Uchiumi, Seiji Naito

    ENDOCRINE-RELATED CANCER   22 ( 6 )   889 - 900   2015.12

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    DOI: 10.1530/ERC-15-0225

  • SRD5A gene polymorphism in Japanese men predicts prognosis of metastatic prostate cancer with androgen-deprivation therapy Invited Reviewed International journal

    Shiota M, Akira Yokomizo, Takeshi Uchiumi, Seiji Naito

    EUROPEAN JOURNAL OF CANCER   51 ( 14 )   1962 - 1969   2015.9

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    DOI: 10.1016/j.ejca.2015.06.122

  • Analysis of the Neuraminidase Amino Acid Sequences of Influenza A/H1N1pdm09, A/H3N2, and B Viruses Isolated from Influenza Patients in the 2013/14 Japanese Influenza Season Reviewed

    Hideyuki Ikematsu, Yong Jeong, Kenjiro Shirane, Hidehiro Toh, Hiroyuki Sasaki, Yui Koga, Shinya Matsumoto, Taeko Hotta, Takeshi Uchiumi, Dongchon Kang

    Fukuoka Acta Medica   106 ( 8 )   231 - 239   2015.8

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  • A CADASIL-Like Case with a Novel Noncysteine Mutation of the NOTCH3 Gene and Granular Deposits in the Renal Arterioles Invited Reviewed International journal

    Kuniyuki Nakamura, Ago T, Takeshi Uchiumi, Kitazono T

    Case Rep Neurol Med   2015.3

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  • Analysis of influenza A/H3N2 neuraminidase genes obtained from influenza patients in the 2011/12 and 2012/13 seasons in Japan Reviewed

    Hideyuki Ikematsu, Yong Jeong, Kenjiro Shirane, Hidehiro Toh, Hiroyuki Sasaki, Yui Koga, Michiyo Urata, Taeko Hotta, Takeshi Uchiumi, Dongchon Kang

    Fukuoka Acta Medica   106 ( 1 )   16 - 22   2015.1

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  • Tor and the Sin3-Rpd3 complex regulate expression of the mitophagy receptor protein Atg32 in yeast Reviewed International journal

    127   3184 - 3196   2014.5

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    DOI: 10.1242/jcs.153254 jcs.153254 [pii]

  • PMA induces androgen receptor downregulation and cellular apoptosis in prostate cancer cells Reviewed

    Itsumi, M., Shiota, M., Yokomizo, A, Takeshi Uchiumi

    53 ( 1 )   31 - 41   2014.5

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    DOI: 10.1530/JME-13-0303 JME-13-0303 [pii]

  • Inhibition of RSK/YB-1 signaling enhances the anti-cancer effect of enzalutamide in prostate cancer Reviewed International journal

    Masaki Shiota, Akira Yokomizo, Takeshi Uchiumi, Seiji Naito

    74 ( 9 )   959 - 969   2014.4

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    DOI: 10.1002/pros.22813

  • PMA induces androgen receptor downregulation and cellular apoptosis in prostate cancer cells Reviewed

    Momoe Itsumi, masaki shiota, Akira Yokomizo, ario takeuchi, Eiji Kashiwagi, Takashi Dejima, Junichi Inokuchi, Katsunori Tatsugami, Takeshi Uchiumi, Seiji Naito

    Journal of Molecular Endocrinology   53 ( 1 )   31 - 41   2014.4

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    DOI: 10.1530/JME-13-0303

  • Inhibition of protein kinase C/twist1 signaling augments anticancer effects of androgen deprivation and enzalutamide in prostate cancer Reviewed

    masaki shiota, Akira Yokomizo, ario takeuchi, Kenjiro Imada, Eiji Kashiwagi, Yoohyun Song, Junichi Inokuchi, Katsunori Tatsugami, Takeshi Uchiumi, Seiji Naito

    Clinical Cancer Research   20 ( 4 )   951 - 961   2014.2

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    DOI: 10.1158/1078-0432.CCR-13-1809

  • EP2 signaling mediates suppressive effects of celecoxib on androgen receptor expression and cell proliferation in prostate cancer Reviewed

    Eiji Kashiwagi, masaki shiota, Akira Yokomizo, Junichi Inokuchi, Takeshi Uchiumi, S. Naito

    Prostate Cancer and Prostatic Diseases   17 ( 1 )   10 - 17   2014.1

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    DOI: 10.1038/pcan.2013.53

  • Targeting ribosomal S6 kinases/Y-box binding protein-1 signaling improves cellular sensitivity to taxane in prostate cancer Reviewed

    masaki shiota, Momoe Itsumi, Akira Yokomizo, ario takeuchi, Kenjiro Imada, Eiji Kashiwagi, Junichi Inokuchi, Katsunori Tatsugami, Takeshi Uchiumi, Seiji Naito

    Prostate   74 ( 8 )   829 - 838   2014.1

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    DOI: 10.1002/pros.22799

  • Tor and the Sin3-Rpd3 complex regulate expression of the mitophagy receptor protein Atg32 in yeast Reviewed

    Masamune Aihara, Xiulian Jin, Yusuke Kurihara, Yutaka Yoshida, Yuichi Matsushima, Masahide Oku, Yuko Hirota, Tetsu Saigusa, Yoshimasa Aoki, Takeshi Uchiumi, Tadashi Yamamoto, Yasuyoshi Sakai, Dongchon Kang, Tomotake Kanki

    Journal of Cell Science   127 ( 14 )   3184 - 3196   2014.1

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    DOI: 10.1242/jcs.153254

  • Twist1 and Y-box-binding protein-1 are potential prognostic factors in bladder cancer Reviewed

    Yoo Hyun Song, masaki shiota, Akira Yokomizo, Takeshi Uchiumi, Keijiro Kiyoshima, Kentaro Kuroiwa, Yoshinao Oda, Seiji Naito

    Urologic Oncology: Seminars and Original Investigations   32 ( 1 )   31.e1 - 31.e7   2014.1

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    DOI: 10.1016/j.urolonc.2012.11.003

  • Casein kinase 2 is essential for mitophagy Reviewed International journal

    EMBO Reports   14   788 - 794   2013.10

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  • Identification of Novel Amelogenin-Binding Proteins by Proteomics Analysis Reviewed

    Takao Fukuda, Terukazu Sanui, Kyousuke Toyoda, Urara Tanaka, Takaharu Taketomi, Takeshi Uchiumi, Fusanori Nishimura

    PloS one   8 ( 10 )   2013.10

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    DOI: 10.1371/journal.pone.0078129

  • Casein kinase 2 is essential for mitophagy Reviewed

    Tomotake Kanki, Yusuke Kurihara, Xiulian Jin, Tadahiro Goda, Yusuke Ono, Masamune Aihara, Yuko Hirota, Tetsu Saigusa, Yoshimasa Aoki, Takeshi Uchiumi, Dongchon Kang

    EMBO Reports   14 ( 9 )   788 - 794   2013.9

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    DOI: 10.1038/embor.2013.114

  • Interaction between docetaxel resistance and castration resistance in prostate cancer Implications of Twist1, YB-1, and androgen receptor Reviewed

    masaki shiota, Eiji Kashiwagi, Akira Yokomizo, ario takeuchi, Takashi Dejima, Yoohyun Song, Katsunori Tatsugami, Junichi Inokuchi, Takeshi Uchiumi, Seiji Naito

    Prostate   73 ( 12 )   1336 - 1344   2013.9

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    DOI: 10.1002/pros.22681

  • Prostaglandin receptor EP3 mediates growth inhibitory effect of aspirin through androgen receptor and contributes to castration resistance in prostate cancer cells Reviewed

    Eiji Kashiwagi, masaki shiota, Akira Yokomizo, Momoe Itsumi, Junichi Inokuchi, Takeshi Uchiumi, Seiji Naito

    Endocrine-Related Cancer   20 ( 3 )   431 - 441   2013.6

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    DOI: 10.1530/ERC-12-0344

  • Dihydro-orotate dehydrogenase is physically associated with the respiratory complex and its loss leads to mitochondrial dysfunction Reviewed

    Jing Xian Fang, Takeshi Uchiumi, Mikako Yagi, Shinya Matsumoto, Rie Amamoto, Shinya Takazaki, Haruyoshi Yamaza, Kazuaki Nonaka, Dongchon Kang

    Bioscience Reports   33 ( 2 )   217 - 227   2013.5

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    DOI: 10.1042/BSR20120097

  • Mutation and functional analysis of ABCC2/multidrug resistance protein 2 in a Japanese patient with Dubin-Johnson syndrome Reviewed

    Takeshi Uchiumi, Hiroyuki Tanamachi, Kajiyo Kuchiwaki, Mitsuharu Kajita, Shinya Matsumoto, Mikako Yagi, Tomotake Kanki, Dongchon Kang

    Hepatology Research   43 ( 5 )   569 - 575   2013.5

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    DOI: 10.1111/j.1872-034X.2012.01103.x

  • The clinical evaluation of high risk human papillomavirus (HPV) genotyping in cervical cancer tissue using a novel electric DNA chip-based diagnostic system Reviewed

    Yui Koga, Michiyo Urata, Takeshi Uchiumi, Osamu Sato, Hiroaki Kobayashi, Dongchon Kang

    Japanese Journal of Clinical Chemistry   42 ( 2 )   140 - 145   2013.4

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  • Dihydroorotate dehydrogenase is physically associated with the respiratory complex and its loss leads to mitochondrial dysfunction. Reviewed International journal

    Bioscience report   33   217 - 227   2013.3

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  • Human heterochromatin protein 1 Isoforms regulate androgen receptor signaling in prostate cancer Reviewed

    Momoe Itsumi, masaki shiota, Akira Yokomizo, Eiji Kashiwagi, ario takeuchi, Katsunori Tatsugami, Junichi Inokuchi, Yoohyun Song, Takeshi Uchiumi, Seiji Naito

    Journal of Molecular Endocrinology   50 ( 3 )   401 - 409   2013.3

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    DOI: 10.1530/JME-13-0024

  • [The functional and pathological analysis of mitochondrial protein p32]. Reviewed

    Takeshi Uchiumi, Dongchon Kang

    Unknown Journal   61 ( 6 )   493 - 500   2013.1

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  • Dihydro-orotate dehydrogenase is physically associated with the respiratory complex and its loss leads to mitochondrial dysfunction. Reviewed

    Jing Xian Fang, Takeshi Uchiumi, Mikako Yagi, Shinya Matsumoto, Rie Amamoto, Shinya Takazaki, Haruyoshi Yamaza, Kazuaki Nonaka, Dongchon Kang

    Bioscience Reports   33 ( 2 )   2013

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  • Protein instability and functional defects caused by mutations of dihydro-orotate dehydrogenase in Miller syndrome patients Reviewed

    Jing Xian Fang, Takeshi Uchiumi, Mikako Yagi, Shinya Matsumoto, Rie Amamoto, Toshiro Saito, Shinya Takazaki, Tomotake Kanki, Haruyoshi Yamaza, Kazuaki Nonaka, Dongchon Kang

    Bioscience Reports   32 ( 6 )   631 - 639   2012.12

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    DOI: 10.1042/BSR20120046

  • Protein instability and functional defects caused by mutations of dihydro-orotate dehydrogenase in Miller syndrome patients. Reviewed International journal

    Bioscience Reports   32   631 - -639   2012.11

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  • P32/gC1qR is indispensable for fetal development and mitochondrial translation Importance of its RNA-binding ability Reviewed

    Mikako Yagi, Takeshi Uchiumi, Shinya Takazaki, Bungo Okuno, Masatoshi Nomura, Shin Ichi Yoshida, Tomotake Kanki, Dongchon Kang

    Nucleic Acids Research   40 ( 19 )   9717 - 9737   2012.10

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    DOI: 10.1093/nar/gks774

  • Downregulation of phosphodiesterase 4B (PDE4B) activates protein kinase A and contributes to the progression of prostate cancer Reviewed

    Eiji Kashiwagi, masaki shiota, Akira Yokomizo, Momoe Itsumi, Junichi Inokuchi, Takeshi Uchiumi, Seiji Naito

    Prostate   72 ( 7 )   741 - 751   2012.5

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    DOI: 10.1002/pros.21478

  • The role of TFAM-associated proteins in mitochondrial RNA metabolism Reviewed

    Takeshi Uchiumi, Dongchon Kang

    Biochimica et Biophysica Acta - General Subjects   1820 ( 5 )   565 - 570   2012.5

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    DOI: 10.1016/j.bbagen.2011.08.014

  • Localization of mRNAs encoding human mitochondrial oxidative phosphorylation proteins Reviewed

    Shinya Matsumoto, Takeshi Uchiumi, Toshiro Saito, Mikako Yagi, Shinya Takazaki, Tomotake Kanki, Dongchon Kang

    Mitochondrion   12 ( 3 )   391 - 398   2012.5

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    DOI: 10.1016/j.mito.2012.02.004

  • Ribonucleoprotein Y-box-binding protein-1 regulates mitochondrial oxidative phosphorylation (OXPHOS) protein expression after serum stimulation through binding to OXPHOS mRNA Reviewed

    Shinya Matsumoto, Takeshi Uchiumi, Hiroyuki Tanamachi, Toshiro Saito, Mikako Yagi, Shinya Takazaki, Tomotake Kanki, Dongchon Kang

    Biochemical Journal   443 ( 2 )   573 - 584   2012.4

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    DOI: 10.1042/BJ20111728

  • Localization of mRNAs encoding human mitochondrial oxidative phosphorylation proteins. Reviewed International journal

    Matsumoto S, Uchiumi T, Saito T, Yagi M, Takazaki S, Kanki T, Kang D

    Mitochondrion   12   2012.3

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  • Antioxidant therapy alleviates oxidative stress by androgen deprivation and prevents conversion from androgen dependent to castration resistant prostate cancer Reviewed

    masaki shiota, Yoohyun Song, ario takeuchi, Akira Yokomizo, Eiji Kashiwagi, Kentaro Kuroiwa, Katsunori Tatsugami, Takeshi Uchiumi, Yoshinao Oda, Seiji Naito

    Journal of Urology   187 ( 2 )   707 - 714   2012.2

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    DOI: 10.1016/j.juro.2011.09.147

  • Mitophagy plays an essential role in reducing mitochondrial production of reactive oxygen species and mutation of mitochondrial DNA by maintaining mitochondrial quantity and quality in yeast Reviewed

    Yusuke Kurihara, Tomotake Kanki, Yoshimasa Aoki, Yuko Hirota, Tetsu Saigusa, Takeshi Uchiumi, Dongchon Kang

    Journal of Biological Chemistry   287 ( 5 )   3265 - 3272   2012.1

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    DOI: 10.1074/jbc.M111.280156

  • Phosphorylation of serine 114 on Atg32 mediates mitophagy Reviewed

    Yoshimasa Aoki, Tomotake Kanki, Yuko Hirota, Yusuke Kurihara, Tetsu Saigusa, Takeshi Uchiumi, Dongchon Kang

    Molecular biology of the cell   22 ( 17 )   3206 - 3217   2011.9

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    DOI: 10.1091/mbc.E11-02-0145

  • YB-1 bridges neural stem cells and brain tumor-initiating cells via its roles in differentiation and cell growth. Reviewed International journal

    Fotovati A, Abu-Ali S, Wang PS, Deleyrolle LP, Lee C, Triscott J, Chen JY, Franciosi S, Nakamura Y, Sugita Y, Uchiumi T, Kuwano M, Leavitt BR, Singh SK, Jury A, Jones C, Wakimoto H, Reynolds BA, Pallen CJ, Dunn SE.

    Cancer Res   71   2011.8

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  • YB-1 bridges neural stem cells and brain tumor-initiating cells via its roles in differentiation and cell growth Reviewed

    Abbas Fotovati, Samah Abu-Ali, Pei Shan Wang, Loic P. Deleyrolle, Cathy Lee, Joanna Triscott, James Y. Chen, Sonia Franciosi, Yasuhiro Nakamura, Yasuo Sugita, Takeshi Uchiumi, Michihiko Kuwano, Blair R. Leavitt, Sheila K. Singh, Alexa Jury, Chris Jones, Hiroaki Wakimoto, Brent A. Reynolds, Catherine J. Pallen, Sandra E. Dunn

    Cancer Research   71 ( 16 )   5569 - 5578   2011.8

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    DOI: 10.1158/0008-5472.CAN-10-2805

  • Y-box binding protein-1 promotes castration-resistant prostate cancer growth via androgen receptor expression Reviewed

    masaki shiota, ario takeuchi, YooHyun Song, Akira Yokomizo, Eiji Kashiwagi, Takeshi Uchiumi, Kentaro Kuroiwa, Katsunori Tatsugami, Naohiro Fujimoto, Yoshinao Oda, Seiji Naito

    Endocrine-Related Cancer   18 ( 4 )   505 - 517   2011.8

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    DOI: 10.1530/ERC-11-0017

  • Mitophagy plays an essential role in reducing mitochondrial production of reactive oxygen species and mutation of mitochondrial DNA by maintaining mitochondrial quantity and quality in yeast Reviewed International journal

    Kurihara Y, Kanki T, Aoki Y, Hirota Y, Saigusa T, Uchiumi T, Kang D

    J Biol Chem   287   2011.8

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  • The role of TFAM-associated proteins in mitochondrial RNA metabolism. Invited Reviewed International journal

    Uchiumi T, Kang D.

    Biochim Biophys Acta.   2011.8

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  • Phosphorylation of Serine 114 on Atg32 mediates mitophagy. Reviewed International journal

    Aoki Y, Kanki T, Hirota Y, Kurihara Y, Saigusa T, Uchiumi T, Kang D

    Mol Biol Cell   22   2011.8

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  • Peroxiredoxin 2 in the nucleus and cytoplasm distinctly regulates androgen receptor activity in prostate cancer cells Reviewed

    masaki shiota, Akira Yokomizo, Eiji Kashiwagi, ario takeuchi, Naohiro Fujimoto, Takeshi Uchiumi, Seiji Naito

    Free Radical Biology and Medicine   51 ( 1 )   78 - 87   2011.7

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    DOI: 10.1016/j.freeradbiomed.2011.04.001

  • Twist1 and Y-box-binding protein-1 promote malignant potential in bladder cancer cells Reviewed

    masaki shiota, Akira Yokomizo, Momoe Itsumi, Takeshi Uchiumi, Yasuhiro Tada, Yoohyun Song, Eiji Kashiwagi, Daisuke Masubuchi, Seiji Naito

    BJU international   108 ( 2 B )   2011.7

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    DOI: 10.1111/j.1464-410X.2010.09810.x

  • Peroxiredoxin 2 in the nucleus and cytoplasm distinctly regulates androgen receptor activity in prostate cancer cells. Reviewed International journal

    Shiota M, Yokomizo A, Kashiwagi E, Takeuchi A, Fujimoto N, Uchiumi T, Naito S.

    Free Radic Biol Med   51   2011.5

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  • Y-box binding protein-1 promotes castration-resistant prostate cancer growth via androgen receptor expression. Reviewed International journal

    Shiota M, Takeuchi A, Song Y, Yokomizo A, Kashiwagi E, Uchiumi T, Kuroiwa K, Tatsugami K, Fujimoto N, Oda Y, Naito S.

    Endocr Relat Cancer.   18   2011.5

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  • Mitochondrial p32/C1QBP is highly expressed in prostate cancer and is associated with shorter PSA relapse time after radical prostatectomy. Reviewed International journal

    Amamoto R, Yagi M, Song Y, Oda Y, Tsuneyoshi M, Naito S, Yokomizo A, Kuroiwa K, Tokunaga S, Kato S, Hiura H, Samori T, Kang D, Uchiumi T.

    Cancer Science   2011.3

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  • Mitochondrial p32/C1QBP is highly expressed in prostate cancer and is associated with shorter prostate-specific antigen relapse time after radical prostatectomy Reviewed

    Rie Amamoto, Mikako Yagi, Yoo Hyun Song, Yoshinao Oda, Masazumi Tsuneyoshi, Seiji Naito, Akira Yokomizo, Kentaro Kuroiwa, Shoji Tokunaga, Seiji Kato, Hisahide Hiura, Tomohiro Samori, Dongchon Kang, Takeshi Uchiumi

    Cancer Science   102 ( 3 )   639 - 647   2011.3

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    DOI: 10.1111/j.1349-7006.2010.01828.x

  • Foxo3a suppression of urothelial cancer invasiveness through twist1, Y-box-binding protein 1, and E-cadherin regulation Reviewed

    masaki shiota, Yoo Hyun Song, Akira Yokomizo, Keijiro Kiyoshima, Yasuhiro Tada, Hiroshi Uchino, Takeshi Uchiumi, Junichi Inokuchi, Yoshinao Oda, Kentaro Kuroiwa, Katsunori Tatsugami, Seiji Naito

    Clinical Cancer Research   16 ( 23 )   5654 - 5663   2010.12

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    DOI: 10.1158/1078-0432.CCR-10-0376

  • Role of ZNF143 in tumor growth through transcriptional regulation of DNA replication and cell-cycle-associated genes Reviewed

    Hiroto Izumi, Tetsuro Wakasugi, Shohei Shimajiri, Akihide Tanimoto, Yasuyuki Sasaguri, Eiji Kashiwagi, Yoshihiro Yasuniwa, Masaki Akiyama, Bin Han, Ying Wu, Takeshi Uchiumi, Tokuzo Arao, Kazuto Nishio, Ryuta Yamazaki, Kimitoshi Kohno

    Cancer Science   101 ( 12 )   2538 - 2545   2010.12

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    DOI: 10.1111/j.1349-7006.2010.01725.x

  • Role of ZNF143 in tumor growth through transcriptional regulation of DNA replication and cell-cycle-associated genes Reviewed International journal

    Izumi H, Wakasugi T, Shimajiri S, Tanimoto A, Sasaguri Y, Kashiwagi E, Yasuniwa Y, Akiyama M, Han B, Wu Y, Uchiumi T, Arao T, Nishio K, Yamazaki R, Kohno K

    Cancer Science   101   2010.9

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  • Twist1 and Y-box-binding protein-1 promote malignant potential in bladder cancer cells. Reviewed International journal

    Twist1 and Y-box-binding protein-1 promote malignant potential in bladder cancer cells.

    BJU Int   2010.9

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  • P300/CBP-associated factor regulates Y-box binding protein-1 expression and promotes cancer cell growth, cancer invasion and drug resistance Reviewed

    masaki shiota, Akira Yokomizo, Yasuhiro Tada, Takeshi Uchiumi, Junichi Inokuchi, Katsunori Tatsugami, Kentaro Kuroiwa, Ken Yamamoto, Narihito Seki, Seiji Naito

    Cancer Science   101 ( 8 )   1797 - 1806   2010.8

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    DOI: 10.1111/j.1349-7006.2010.01598.x

  • Sensitivity of doxorubicin-resistant cells to sorafenib Possible role for inhibition of eukaryotic initiation factor-2α phosphorylation Reviewed

    37 ( 2 )   509 - 517   2010.8

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    Patients with advanced cancer including breast cancer, hepatocellular cancer and urothelial cancer frequently receive a chemotherapy regimen containing doxorubicin. However, doxorubicin-resistance is a major obstacle for cancer chemotherapy. Recently, several molecular-targeted agents have become available. Sorafenib (BAY 43-9006) is known to target multiple kinases and has demonstrated activity in renal cell and hepatocellular cancer. In this study, sorafenib was found to inhibit phosphorylation of the eukaryotic initiation factor-2α (eIF2α), induce cell cycle arrest at G2 phase and increase cellular apoptosis in doxorubicin-resistant human urothelial cell lines. An eIF2α kinase, PERK was responsible for eIF2α phosphorylation and PERK knockdown induced cellular apoptosis similar to sorafenib treatment in doxorubicin-resistant cancer cells. Furthermore, sorafenib sensitized doxorubicin-resistant cancer cells, but not their parental cells to oxidative stress exerted by both hydrogen peroxide and doxorubicin. In addition, PERK knockdown sensitized doxorubicin-resistant cancer cells to oxidative stress. In conclusion, PERK inhibition using sorafenib with or without doxorubicin might be a promising therapeutic approach for doxorubicin-resistant cancers retaining high phosphorylation levels of eIF2α.

    DOI: 10.3892/ijo-0000700

  • P300/CBP-associated factor regulates Y-box binding protein-1 expression and promotes cancer cell growth, cancer invasion and drug resistance. Reviewed International journal

    Shiota M, Yokomizo A, Tada Y, Uchiumi T, Inokuchi J, Tatsugami K, Kuroiwa K, Yamamoto K, Seki N, Naito S.

    Cancer Science   101   2010.7

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  • The maintenance factor of mitochondrial DNA and cancer Reviewed

    Takeshi Uchiumi

    Japanese Journal of Clinical Chemistry   39 ( 3 )   253 - 259   2010.7

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  • Japanese Journal of Clinical Chemistry Preface Reviewed

    Takeshi Uchiumi

    Japanese Journal of Clinical Chemistry   39 ( 3 )   2010.7

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  • Sensitivity of doxorubicin-resistant cells to sorafenib: possible role for inhibition of eukaryotic initiation factor-2alpha phosphorylation Reviewed International journal

    Shiota M, Eto M, Yokomizo A, Tada Y, Takeuchi A, Itsumi M, Tatsugami K, Uchiumi T, Naito S

    Int J Oncol   37   2010.7

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  • Foxo3a suppression of urothelial cancer invasiveness through Twist1, Y-box-binding protein 1, and E-cadherin regulation Reviewed International journal

    Shiota M, Song Y, Yokomizo A, Kiyoshima K, Tada Y, Uchino H, Uchiumi T, Inokuchi J, Oda Y, Kuroiwa K, Tatsugami K, Naito S

    Clin. Cancer Res   16   2010.7

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  • Sorafenib with doxorubicin augments cytotoxicity to renal cell cancer through PERK inhibition Reviewed International journal

    Shiota, M. Eto, M. Yokomizo, A. Tada, Y. Takeuchi, A. Masubuchi, D. Inokuchi, J. Tatsugami, K. Kuroiwa, K. Uchiumi, T. Seki, N. Naito, S.

    Int J Oncol   36 ( 6 )   2010.6

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  • Sorafenib with doxorubicin augments cytotoxicity to renal cell cancer through PERK inhibition Reviewed

    Masaki Shiota, Masatoshi Eto, Akira Yokomizo, Yasuhiro Tada, Ario Takeuchi, Daisuke Masubuchi, Junichi Inokuchi, Katsunori Tatsugami, Kentaro Kuroiwa, Takeshi Uchiumi, Narihito Seki, Seiji Naito

    International journal of oncology   36 ( 6 )   1521 - 1531   2010.6

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    DOI: 10.3892/ijo-00000639

  • Human heterochromatin protein 1 isoform HP1β enhances androgen receptor activity and is implicated in prostate cancer growth Reviewed

    17 ( 2 )   455 - 467   2010.6

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    There are currently few successful therapies for castration-resistant prostate cancer (CRPC). CRPC is thought to result from augmented activation of the androgen/androgen receptor (AR) signaling pathway, which could be enhanced by AR cofactors. In this study, heterochromatin protein 1β (HP1β), but not HP1α or HP1γ was found to be an AR cofactor. HP1β interacted with the AR, and enhanced the DNA-binding ability of AR to androgen-responsive element in the prostate-specific antigen enhancer and promoter regions, and to increase the transcription of AR target genes. In prostate cancer (PCa) tissues, HP1β expressions correlated with Gleason score and tri-methylation levels of histone H3 lysine 9. Silencing of HP1β suppressed the growth of AR-expressing PCa cells by inducing cell-cycle arrest at the G1 phase, similar to inhibition of androgen/AR signaling. Furthermore, HP1β was overexpressed in castration-resistant LNCaP derivative CxR cells, and HP1β knockdown also suppressed the cell growth in CxR cells. These findings indicate that HP1β is involved in the proliferation of AR-expressing PCa cells and progression to CRPC as an AR coactivator. Modulation of HP1β expression or function might be a useful strategy for developing novel therapeutics for PCa, even in CRPC.

    DOI: 10.1677/ERC-09-0321

  • ERAL1 is associated with mitochondrial ribosome and elimination of ERAL1 leads to mitochondrial dysfunction and growth retardation Reviewed International journal

    Uchiumi, T. Ohgaki, K. Yagi, M. Aoki, Y. Sakai, A. Matsumoto, S. Kang, D.

    Nucleic Acids Res   2010.5

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    ERAL1, a homologue of Era protein in Escherichia coli, is a member of conserved GTP-binding proteins with RNA-binding activity. Depletion of prokaryotic Era inhibits cell division without affecting chromosome segregation. Previously, we isolated ERAL1 protein as one of proteins which were associated with mitochondrial transcription factor A by using immunoprecipitation. In this study, we analysed the localization and function of ERAL1 in mammalian cells. ERAL1 was localized in mitochondrial matrix and associated with mitoribosomal proteins including the 12S rRNA. siRNA knockdown of ERAL1 decreased mitochondrial translation, caused redistribution of ribosomal small subunits and reduced 12S rRNA. The knockdown of ERAL1 in human HeLa cells elevated mitochondrial superoxide production and slightly decreased mitochondrial membrane potential. The knockdown inhibited the growth of HeLa cells with an accumulation of apoptotic cells. These results suggest that ERAL1 is localized in a small subunit of the mitochondrial ribosome, plays an important role in the small ribosomal constitution, and is also involved in cell viability.

  • Foxo3a expression and acetylation regulate cancer cell growth and sensitivity to cisplatin Reviewed

    Masaki Shiota, Akira Yokomizo, Eiji Kashiwagi, Yasuhiro Tada, Junichi Inokuchi, Katsunori Tatsugami, Kentaro Kuroiwa, Takeshi Uchiumi, Narihito Seki, Seiji Naito

    Cancer Science   101 ( 5 )   1177 - 1185   2010.5

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    DOI: 10.1111/j.1349-7006.2010.01503.x

  • Human heterochromatin protein 1 isoform HP1{beta} enhances androgen receptor activity and is implicated in prostate cancer growth Reviewed International journal

    Shiota, M. Song, Y. Yokomizo, A. Tada, Y. Kuroiwa, K. Eto, M. Oda, Y. Inokuchi, J. Uchiumi, T. Fujimoto, N. Seki, N. Naito, S.

    Endocr Relat Cancer   2010.5

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  • Foxo3a expression and acetylation regulate cancer cell growth and sensitivity to cisplatin Reviewed International journal

    Shiota, M. Yokomizo, A. Kashiwagi, E. Tada, Y. Inokuchi, J. Tatsugami, K. Kuroiwa, K. Uchiumi, T. Seki, N. Naito, S.

    Cancer Sci   2010.5

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  • Peroxisome proliferator-activated receptor gamma coactivator-1alpha interacts with the androgen receptor (AR) and promotes prostate cancer cell growth by activating the AR Reviewed International journal

    Shiota, M. Yokomizo, A. Tada, Y. Inokuchi, J. Tatsugami, K. Kuroiwa, K. Uchiumi, T. Fujimoto, N. Seki, N. Naito, S.

    Mol Endocrinol   2010.5

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  • Tip60 promotes prostate cancer cell proliferation by translocation of androgen receptor into the nucleus Reviewed International journal

    Shiota, M. Yokomizo, A. Masubuchi, D. Tada, Y. Inokuchi, J. Eto, M. Uchiumi, T. Fujimoto, N. Naito, S.

    Prostate   70 ( 5 )   2010.4

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  • Tip60 promotes prostate cancer cell proliferation by translocation of androgen receptor into the nucleus Reviewed

    Masaki Shiota, Akira Yokomizo, Daisuke Masubuchi, Yasuhiro Tada, Junichi Inokuchi, Masatoshi Eto, Takeshi Uchiumi, Naohiro Fujimoto, Seiji Naito

    Prostate   70 ( 5 )   540 - 554   2010.4

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    DOI: 10.1002/pros.21088

  • ERAL1 is associated with mitochondrial ribosome and elimination of ERAL1 leads to mitochondrial dysfunction and growth retardation Reviewed

    Takeshi Uchiumi, Kippei Ohgaki, Mikako Yagi, Yoshimasa Aoki, Aya Sakai, Shinya Matsumoto, Dongchon Kang

    Nucleic acids research   38 ( 16 )   5554 - 5568   2010.4

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    DOI: 10.1093/nar/gkq305

  • Castration resistance of prostate cancer cells caused by castration-induced oxidative stress through Twist1 and androgen receptor overexpression Reviewed International journal

    Shiota, M. Yokomizo, A. Tada, Y. Inokuchi, J. Kashiwagi, E. Masubuchi, D. Eto, M. Uchiumi, T. Naito, S.

    Oncogene   29 ( 2 )   2010.1

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  • Peroxisome proliferator-activated receptor γ coactivator-1α interacts with the androgen receptor (AR) and promotes prostate cancer cell growth by activating the AR Reviewed

    24 ( 1 )   114 - 127   2010.1

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    There are currently few successful therapies for castration-resistant prostate cancer (CRPC). CRPC is thought to result from augmented activation of the androgen/androgen receptor (AR) signaling pathway, which could be enhanced by AR cofactors. In this study, peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) was found to be an AR cofactor. PGC-1α interacted with the N-terminal domain of AR, was involved in the N- and C-terminal interaction of AR, and enhanced the DNA-binding ability of AR to androgen-responsive elements in the prostate-specific antigen enhancer and promoter regions to increase the transcription of AR target genes. Silencing of PGC-1α suppressed cell growth of AR-expressing prostate cancer (PCa) cells by inducing cell-cycle arrest at the G1 phase, similar to inhibition of androgen/AR signaling. Furthermore, PGC-1α knock-down also suppressed cell growth in the castration-resistant LNCaP-derivatives. These findings indicate that PGC-1α is involved in the proliferation of AR-expressing PCa cells by acting as an AR coactivator. Modulation of PGC-1α expression or function may offer a useful strategy for developing novel therapeutics for PCa, including CRPC, which depends on AR signaling by over-expressing AR and its coactivators.

    DOI: 10.1210/me.2009-0302

  • Castration resistance of prostate cancer cells caused by castration-induced oxidative stress through Twist1 and androgen receptor overexpression Reviewed

    M. Shiota, A. Yokomizo, Y. Tada, J. Inokuchi, E. Kashiwagi, D. Masubuchi, M. Eto, T. Uchiumi, S. Naito

    Oncogene   29 ( 2 )   237 - 250   2010.1

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    DOI: 10.1038/onc.2009.322

  • Cisplatin sensitivity transcriptional factor and mitochondrial DNA maintenance protein Reviewed

    Takeshi Uchiumi, Donchon Knag

    Rinsho byori. The Japanese journal of clinical pathology   57 ( 10 )   978 - 986   2009.10

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  • DNA conformation-dependent activities of human mitochondrial RNA polymerase Reviewed International journal

    Fukuoh, A. Ohgaki, K. Hatae, H. Kuraoka, I. Aoki, Y. Uchiumi, T. Jacobs, H. T.Kang, D.

    Genes Cells   14 ( 8 )   2009.8

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  • DNA conformation-dependent activities of human mitochondrial RNA polymerase Reviewed

    Atsushi Fukuoh, Kippei Ohgaki, Hinako Hatae, Isao Kuraoka, Yoshimasa Aoki, Takeshi Uchiumi, Howard T. Jacobs, Dongchon Kang

    Genes to Cells   14 ( 8 )   1029 - 1042   2009.8

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    DOI: 10.1111/j.1365-2443.2009.01328.x

  • DNA topoisomerase inhibitor, etoposide, enhances GC-box-dependent promoter activity via Sp1 phosphorylation Reviewed International journal

    Niina I, Uchiumi T, Izumi H,Torigoe T, Wakasugi T, Igarashi T, Miyamoto N, Onitsuka T, Shiota M, Okayasu R, Chijiiwa K, and Kohno K

    Cancer Science   2007.10

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  • A new mechanism for primary resistance to gefitinib in lung adenocarcinoma: the role of a novel G796A mutation in exon 20 of EGFR Reviewed International journal

    Uramoto H, Uchiumi T, Izumi H, Kohno K, Oyama T, Sugio K, and Yasumoto K

    Anticancer Res,   2007.10

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  • p73gamma transactivates the p21 promoter through preferential interaction with the p300/CBP-associated factor in human prostate cancer cells. Reviewed International journal

    Momii Y, Izumi H, Shiota M, Onistuka T, Abe T, Kobayasi H, Miyamoto N, Uchiumi T, and Kohno K

    Oncology Report   2007.10

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  • ZNF143 interacts with p73 and is involved in cisplatin resistance through the transcriptional regulation of DNA repair genes. Reviewed International journal

    Wakasugi,T., Izumi,H., Uchiumi,T., Suzuki,H., Arao,T., Nishio,K. and Kohno,K

    Oncogene   2007.9

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  • ZNF143 interacts with p73 and is involved in cisplatin resistance through the transcriptional regulation of DNA repair genes Reviewed

    T. Wakasugi, H. Izumi, T. Uchiumi, H. Suzuki, T. Arao, K. Nishio, K. Kohno

    Oncogene   26 ( 36 )   5194 - 5203   2007.8

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    DOI: 10.1038/sj.onc.1210326

  • p73γ transactivates the p21 promoter through preferential interaction with the p300/CBP-associated factor in human prostate cancer cells Reviewed

    18 ( 2 )   411 - 416   2007.8

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    Several p73 variants have been reported with different carboxy-terminal structures and transcriptional activities. We showed that p73γ had stronger transactivation activity than the other splicing variants such as α, β and δ by analysing p21 promoter activity in human prostate cancer PC3 cells. The transactivation activity of p73γ was similar to that of p53 and was enhanced by co-transfection with p300/CBP-associated factor (PCAF). In vitro pull-down assay, p73 variants were able to bind to PCAF with a similar extent. However, in vivo co-immunoprecipitation assays showed that p73γ interacted preferentially with PCAF. Neither in vitro-translated nor in vivo-immunoprecipitated p73γ were able to bind to oligonucleotides containing the p53 consensus binding site. However, p73γ acetylated by PCAF restored DNA binding activity. Differential functions of p73 variants are supposed to be regulated by the structural differences of carboxy-terminal region. Our results revealed that p21 promoter activity was affected by differential interactions of p73 variants with PCAF and its acetylation.

  • A new mechanism for primary resistance to gefitinib in lung adenocarcinoma The role of a novel G796A mutation in exon 20 of EGFR Reviewed

    Hidetaka Uramoto, Takeshi Uchiumi, Hiroto Izumi, Kimitoshi Kohno, Tsunehiro Oyama, Kenji Sugio, Kosei Yasumoto

    Anticancer research   27 ( 4 B )   2297 - 2303   2007.7

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  • Clock and ATF4 transcription system regulates drug resistance in human cancer cell lines Reviewed

    T. Igarashi, H. Izumi, T. Uchiumi, K. Nishio, T. Arao, M. Tanabe, H. Uramoto, K. Sugio, K. Yasumoto, Y. Sasaguri, K. Y. Wang, Y. Otsuji, K. Kohno

    Oncogene   26 ( 33 )   4749 - 4760   2007.7

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    DOI: 10.1038/sj.onc.1210289

  • Clock/ATF4 transcription system regulates multidrug resistance in human cancer cell line. Reviewed International journal

    Igarashi,T., Izumi,H., Uchiumi,T., Nishio,K., Arao,T., Uramoto,H., Sugio,K., Yasumoto,K., Sasaguri,Y., Wang,KY., Otsuji,Y. and Kohno,K.

    Oncogene   2007.6

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  • DNA topoisomerase inhibitor, etoposide, enhances GC-box-dependent promoter activity via Sp1 phosphorylation Reviewed

    Ichiro Niina, Takeshi Uchiumi, Hiroto Izumi, Takayuki Torigoe, Tetsuro Wakasugi, Tomonori Igarashi, Naoya Miyamoto, Takamitsu Onitsuka, Masaki Shiota, Ryuichi Okayasu, Kazuo Chijiiwa, Kimitoshi Kohno

    Cancer Science   98 ( 6 )   858 - 863   2007.6

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    DOI: 10.1111/j.1349-7006.2007.00476.x

  • The pleiotropic function of the YB-1-Translational regulation and its knockout mouse Reviewed

    Takeshi Uchiumi

    Japanese Journal of Clinical Chemistry   36 ( 4 )   296 - 302   2007

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  • YB-1 is important for an early stage embryonic development Neural tube formation and cell proliferation Reviewed

    Takeshi Uchiumi, Abbas Fotovati, Takakazu Sasaguri, Kohtaro Shibahara, Tatsuo Shimada, Takao Fukuda, Takanori Nakamura, Hiroto Izumi, Teruhisa Tsuzuki, Michihiko Kuwano, Kimitoshi Kohno

    Journal of Biological Chemistry   281 ( 52 )   40440 - 40449   2006.12

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    DOI: 10.1074/jbc.M605948200

  • YB-1 is important for an early stage embryonic development: neural tube formation and cell proliferation. Reviewed International journal

    Uchiumi,T., Fotovati,A., Sasaguri,T., Shibahara,K., Shimada,T., Fukuda,T., Nakamura,T., Izumi,H., Tsuzuki,T., Kuwano,M. and Kohno,K

    J. Biol.Chem   2006.11

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  • Inhibitory effects of angiotensin II receptor antagonists and leukotriene receptor antagonists on the transport of human organic anion transporter 4 Reviewed

    Fumiaki Yamashita, Hisakazu Ohtani, Noriko Koyabu, Fumihiko Ushigome, Hiroki Satoh, Hideyasu Murakami, Takeshi Uchiumi, Takanori Nakamura, Michihiko Kuwano, Masayuki Tsujimoto, Yasufumi Sawada

    Journal of Pharmacy and Pharmacology   58 ( 11 )   1499 - 1505   2006.11

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    DOI: 10.1211/jpp.58.11.0011

  • Inhibitory effects of angiotensin II receptor antagonists and leukotriene receptor antagonists on the transport of human organic anion transporter 4. Reviewed International journal

    Yamashita,F., Ohtani,H., Koyabu,N., Ushigome,F., Satoh,H., Murakami,H., Uchiumi,T., Nakamura,T., Kuwano,M., Tsujimoto,M. and Sawada,Y.

    J. Pharm. Pharmacol.   2006.6

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  • Expression of Y-box-binding protein dbpC/contrin, a potentially new cancer/testis antigen. Reviewed International journal

    Kohno,K., Matsuki,Y., Tanimoto,A., Izumi,H., Uchiumi,T., Kohno,K., Shimajiri,S. and Sasaguri,Y.

    Br. J. Cancer   2006.5

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  • Expression and cellular localization of dbpC/Contrin in germ cell tumor cell lines. Reviewed International journal

    Yoshida,T., Izumi,H., Uchiumi,T., Sasaguri,Y., Tanimoto,A., Matsumoto,T., Naito,S. and Kohno,K.

    Biochim. Biophys. Acta   2006.3

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  • Expression of Y-box-binding protein dbpC/contrin, a potentially new cancer/testis antigen Reviewed

    Y. Kohno, Y. Matsuki, A. Tanimoto, H. Izumi, T. Uchiumi, K. Kohno, S. Shimajiri, Y. Sasaguri

    British journal of cancer   94 ( 5 )   710 - 716   2006.3

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    DOI: 10.1038/sj.bjc.6602987

  • Expression and cellular localization of dbpC/Contrin in germ cell tumor cell lines Reviewed

    Takeshi Yoshida, Hiroto Izumi, Takeshi Uchiumi, Yasuyuki Sasaguri, Akihide Tanimoto, Tetsuro Matsumoto, Seiji Naito, Kimitoshi Kohno

    Biochimica et Biophysica Acta - Gene Structure and Expression   1759 ( 1-2 )   80 - 88   2006.1

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    DOI: 10.1016/j.bbaexp.2006.02.005

  • ATP-binding cassette superfamily transporter gene expression in human soft tissue sarcoma. Reviewed International journal

    Oda,Y., Saito,T., Tateishi,N., Ohishi,Y., Tamiya,S.,Yamamoto,H.,Yokoyama,R., Uchiumi,T., Iwamoto,Y., Kuwano,M. and Tsuneyoshi,M

    Int. J. Cancer   114 ( 6 )   854 - 862   2005.11

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    DOI: 10.1002/ijc.20589

  • Transcription factors and drug resistance Reviewed

    Kimitoshi Kohno, Takeshi Uchiumi, Ichiro Niina, Tetsuro Wakasugi, Tomonori Igarashi, Yasutomo Momii, Takeshi Yoshida, Ken Ichi Matsuo, Naoya Miyamoto, Hiroto Izumi

    European Journal of Cancer   41 ( 16 )   2577 - 2586   2005.11

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    DOI: 10.1016/j.ejca.2005.08.007

  • Functional analysis of organic cation transporter 3 expressed in human placenta. Reviewed International journal

    Sata,R., Ohtani,H., Tsujimoto,M., Murakami,H., Koyabu,N., Nakamura,T., Uchiumi,T., Kuwano,M., Nagata,H., Tsukimori,K., Nakano,H. and Sawada,Y

    J. Pharmacol. Exp. Ther.   315 ( 2 )   888 - 895   2005.10

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    DOI: 10.1124/jpet.105.086827

  • The up-regulation of Y-box binding proteins (DNA binding protein A and Y-box binding protein-1) as prognostic markers of hepatocellular carcinoma Reviewed

    Mahmut Yasen, Kazunori Kajino, Sayaka Kano, Hiroshi Tobita, Junji Yamamoto, Takeshi Uchiumi, Shigeyuki Kon, Masahiro Maeda, Gulanbar Obulhasim, Shigeki Arii, Okio Hino

    Clinical Cancer Research   11 ( 20 )   7354 - 7361   2005.10

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    DOI: 10.1158/1078-0432.CCR-05-1027

  • Differential regulation of the human MRP2 and MRP3 gene expression by glucocorticoids. Reviewed International journal

    Pulaski,L., Kania,K., Ratajewski,M., Uchiumi,T., Kuwano,M. and Bartosz,G.

    J. Steroid Biochem. Mol. Biol.   96 ( 3-4 )   229 - 234   2005.8

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    DOI: 10.1016/j.jsbmb.2005.03.004

  • The up-regulation of Y-box binding proteins (DNA binding protein A and Y-box binding protein-1) as prognostic markers of hepatocellular carcinoma. Reviewed International journal

    Yasen,M., Kajino,K., Kano,S., Tobita,H., Yamamoto,J., Uchiumi,T., Kon,S., Maeda,M., Obulhasim,G., Arii,S. and Hino,O.

    Clin. Cancer Res   2005.7

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  • Transcriptional upregulation of the human MRP2 gene expression by serine/threonine protein kinase inhibitors Reviewed

    Journal of Biological Regulators and Homeostatic Agents   19 ( 3-4 )   113 - 119   2005.7

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  • Transcriptional upregulation of the human MRP2 gene expression by serine/threonine protein kinase inhibitors. Reviewed International journal

    Pulaski,L., Szemraj,J., Uchiumi,T., Kuwano,M. and Bartosz,G.

    J. Biol. Regul. Homeost. Agents   19 ( 3-4 )   113 - 119   2005.5

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    Language:English   Publishing type:Research paper (scientific journal)  

  • p130/p107/p105Rb-dependent transcriptional repression during DNA-damage-induced cell-cycle exit at G2 Reviewed

    Mark W. Jackson, Mukesh K. Agarwal, Jinbo Yang, Patrick Bruss, Takeshi Uchiumi, Munna L. Agarwal, George R. Stark, William R. Taylor

    Journal of Cell Science   118 ( 9 )   1821 - 1832   2005.5

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    DOI: 10.1242/jcs.02307

  • Effects of grapefruit juice and orange juice components on P-glycoprotein- and MRP2-mediated drug efflux. Reviewed International journal

    Honda,Y., Ushigome,F., Koyabu,N., Morimoto,S., Shoyama,Y., Uchiumi,T., Kuwano,M., Ohtani H. and Sawada Y.

    Br. J. Pharmacol.   143 ( 7 )   856 - 864   2004.11

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    DOI: 10.1038/sj.bjp.0706008

  • The role of nuclear Y-box binding protein 1 as a global marker in drug resistance Reviewed

    Michihiko Kuwano, Yoshinao Oda, Hiroto Izumi, Song Ju Yang, Takeshi Uchiumi, Yukihide Iwamoto, Masakazu Toi, Teruhiko Fujii, Hideaki Yamana, Hisafumi Kinoshita, Toshiharu Kamura, Masazumi Tsuneyoshi, Kosei Yasumoto, Kimitoshi Kohno

    Molecular cancer therapeutics   3 ( 11 )   1485 - 1492   2004.11

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  • Targeted disruption of one allele of the Y-box binding protein-1 (YB-1) gene in mouse embryonic stem cells and increased sensitivity to cisplatin and mitomycin C Reviewed International journal

    Shibahara,K., Uchiumi,T., Fukuda,T., Kura,S., Tominaga,Y., Maehara,Y., Kohno,K., Nakabeppu,Y., Tsuzuki,T. and Kuwano,M.

    Cancer Sci.   95 ( 4 )   348 - 353   2004.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/j.1349-7006.2004.tb03214.x

  • The basic and clinical implications of ABC transporters, Y-box-binding protein-1 (YB-1) and angiogenesis-related factors in human malignancies Reviewed

    Michihiko Kuwano, Takeshi Uchiumi, Hiroshi Hayakawa, Mayumi Ono, Morimasa Wada, Hiroto Izumi, Kimitoshi Kohno

    Gann Monographs on Cancer Research   52   247 - 259   2004.7

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  • Interleukin-1ß represses MRP2 gene expression through inactivation of interferon regulatory factor 3 in HepG2 cells. Reviewed International journal

    39 ( 6 )   1574 - 1582   2004.4

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    DOI: 10.1002/hep.20216

  • Characterization of the 5′-untranslated region of YB-1 mRNA and autoregulation of translation by YB-1 protein Reviewed

    32 ( 2 )   611 - 622   2004.3

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    The eukaryotic Y-box binding protein YB-1 is involved in various biological processes, including DNA repair, cell proliferation and the regulation of transcription and translation. YB-1 protein is abundant and expressed ubiquitously in human cells, functioning in cell proliferation and transformation. Its concentration is thought to be highly regulated at both the levels of transcription and translation. Therefore, we investigated whether or not the 5′-UTR of YB-1 mRNA affects the translation of YB-1 protein, thus influencing expression levels. Luciferase mRNA ligated to the YB-1 mRNA 5′-UTR was used as a reporter construct. Ligation of the full-length YB-1 5′-UTR (331 bases) enhanced translation as assessed by in vitro and in vivo translation assays. Deletion constructs of the YB-1 5′-UTR also resulted in a higher efficiency of translation, especially in the region mapped to +197 to +331 from the major transcription start site. RNA gel shift assays revealed that the affinity of YB-1 for various 5′-UTR probe sequences was higher for the full-length 5′-UTR than for deleted 5′-UTR sequences. An in vitro translation assay was used to demonstrate that recombinant YB-1 protein inhibited translation of the full-length 5′-UTR of YB-1 mRNA. Thus, our findings provide evidence for the autoregulation of YB-1 mRNA translation via the 5′-UTR.

    DOI: 10.1093/nar/gkh223

  • Genetic polymorphism at the 5′ regulatory region of multidrug resistance 1 (MDR1 ) and its association with interindividual variation of expression level in the colon Reviewed

    2 ( 12 )   1351 - 1359   2003.12

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    The multidrug resistance 1 (MDR1) is a key molecule in determining not only the resistance of cancer cells to anticancer agents but also the disposition of a variety of drugs in intestinal and other tissues. However, the mechanism underlying interindividual variations in levels of MDR1 activity and expression in various tissues remains unclear. We analyzed the nucleotide sequence polymorphisms in the 5V upstream regulatory region of the gene spanning 4 kb from the transcriptional start site of MDR1 and tried to identify any associations between polymorphisms and MDR1 expression. Within that region, we identified eight single nucleotide polymorphisms (SNPs) in the region in the Japanese population. Of the SNPs identified, -2410T>C, -1910T>C, and 692T>C were in perfect linkage disequilibrium. In normal colorectal mucosa, diplotypes at the region showed more significant association with the expression level of MDR1 mRNA than each SNP did. In an in vitro reporter assay, transcription activity of the minor-type construct carrying haplotypes 2 and 3 was significantly lower than that of the major-type construct carrying haplotype 1. We next identified two DNA binding proteins: one protein bound to the nucleotide sequence carrying -692T but not to that carrying -692C and another bound to the nucleotide sequence carrying -2352G but three times weaker than that carrying -2352A. This suggested the significance of SNP at -692 and -2352 of MDR1 in variable expression in the colon interindividually. This is the first report connecting SNPs and interindividual variety of MDR1 expression rationally.

  • Y-box binding protein expression in thyroid neoplasms Its linkage with anaplastic transformation Reviewed

    Yasuhiro Ito, Hiroshi Yoshida, Kotaro Shibahara, Takashi Uruno, Keiichi Nakano, Yuuki Takamura, Akihiro Miya, Kaoru Kobayashi, Tamotsu Yokozawa, Fumio Matsuzuka, Takeshi Uchimi, Michihiko Kuwano, Eiji Miyoshi, Nariaki Matsuura, Kanji Kuma, Akira Miyauchi

    Pathology International   53 ( 7 )   429 - 433   2003.7

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    DOI: 10.1046/j.1440-1827.2003.01494.x

  • Kinetic analysis of P-glycoprotein-mediated transport by using normal human placental brush-border membrane vesicles. Reviewed International journal

    Ushigome, F., Koyabu, N., Satoh, S., Tsukimori, K., Nakano, H., Nakamura, T., Uchiumi, T., Kuwano, M., Ohtani, H., and Sawada, Y

    Pharm Res   20 ( 1 )   38 - 44   2003.1

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    DOI: 10.1023/A:1022290523347

  • Basal membrane localization of MRP1 in human placental trophoblast. Reviewed International journal

    Nagashige M, Ushigome F, Koyabu N, Hirata K, Kawabuchi M, Hirakawa T, Satoh

    Placenta   24 ( 10 )   951 - 958   2003.1

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    DOI: 10.1016/S0143-4004(03)00170-X

  • Identification of domains participating in the substrate specificity and subcellular localization of the multidrug resistance proteins MRP1 and MRP2. Reviewed International journal

    Konno, T., Ebihara, T., Hisaeda, K., Uchiumi, T., Nakamura, T., Shirakusa, T., Kuwano, M., and Wada, M.

    J Biol Chem   278 ( 25 )   22908 - 22917   2003.1

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    DOI: 10.1074/jbc.M302868200

  • Identification of promoter elements responsible for transcriptional inhibition of PLK1 and Topoisomerase IIa genes by p21WAF1/CIP1/SDI1. Reviewed International journal

    Zhu, H. Chang,B.-D. Uchiumi, T. and Roninson, I. B

    Cell cycle,   2002.1

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  • ATP-binding cassette superfamily transporter gene expression in human primary ovarian carcinoma. Reviewed International journal

    Ohishi Y., Oda Y., Uchiumi T., Kobayashi H., Hirakawa T., Miyamoto S.,Kinukawa N.,Nakano H., Kuwano M., and Tsuneyoshi M.

    Clin Cancer Res   8 ( 12 )   3767 - 3775   2002.1

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  • Binding Capacity of Human YB-1 Protein for RNA Containing 8-Oxoguanine. Reviewed International journal

    Hayakawa, H., Uchiumi, T., Fukuda, T., Ashizuka, M., Kohno, K., Kuwano, M., and Sekiguchi, M

    Biochemistry   41 ( 42 )   12739 - 12744   2002.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/bi0201872

  • Functional characterization of mouse cation transporter mOCT2 compared with mOCT1. Reviewed International journal

    Kakehi, M., Koyabu, N., Nakamura, T., Uchiumi, T., Kuwano, M., Ohtani, H., and Sawada, Y

    Biochem Biophys Res Commun   296 ( 3 )   644 - 650   2002.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0006-291X(02)00926-9

  • p73 Interacts with c-Myc to regulate Y-box-binding protein-1 expression Reviewed International journal

    Uramoto, H., Izumi, H., Ise, T., Tada, M., Uchiumi, T., Kuwano, M., Yasumoto, K., Funa, K., and Kohno, K.

    J Biol Chem,   277 ( 35 )   31694 - 31702   2002.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.M200266200

  • Contribution of P-glycoprotein to efflux of ramosetron, a 5-HT3 receptor antagonist, across the blood-brain barrier. Reviewed International journal

    Yamamoto, C., Murakami, H., Koyabu, N., Takanaga, H., Matsuo, H., Uchiumi, T., Kuwano, M., Naito, M., Tsuruo, T., Ohtani, H., and Sawada, Y

    J Pharm Pharmacol   54 ( 8 )   1055 - 1063   2002.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1211/002235702320266208

  • YB-1 binds preferentially to single-stranded nucleic acid and exhibit 3'-5' exonuclease activity. Reviewed International journal

    Izumi, H., Imamura, T., Nagatani, G., Ise, T., Murakami, T., Uramoto, H., Torigoe, T., Ishiguchi, H., Nomoto, M., Okamoto, T., Uchiumi, T., Kuwano, M., Funa, K. and Kohno, K.

    Nucl. Acids Res.   2001.1

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  • Up-regulation of thrombospondin-1 gene by epidermal growth factor and transforming growth facter b in human cancer cells-transcriptional activation and messenger RNA stabilization. Reviewed International journal

    Okamoto, M., Ono, M., Uchiumi, T., Ueno, H., Kohno, K., Sugimachi, K. and Kuwano, M.

    Biochim. Biophys. Acta   2001.1

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  • Nuclear expression of the Y-box binding protein, YB-1, as a novel marker of disease progression in non-small-cell lung cancer. Reviewed International journal

    Shibahara, K., Sugio, K., Osaki, T., Uchiumi, T., Maehara, Y., Kohno, K., Yasumoto, K., Sugimati, K. and Kuwano, M.

    Clinical Cancer Res.   7 ( 10 )   3151 - 3155   2001.1

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  • Structure of a novel multidrug resistance modulator, irciniasulfonic acid, isolated from a marin sponge, Ircinia sp. Reviewed International journal

    Kawakami, A., Tomofumi, M., Higuchi, R., Uchiumi, T., Kuwano, M. and Van Soest, R. W. N.

    Tetrahedron Lett.   42 ( 19 )   3335 - 3337   2001.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0040-4039(01)00426-9

  • Direct interaction of p53 with the Y-box binding protein, YB-1: a mechanism for regulation of human gene expression. Reviewed International journal

    Okamoto, T., Izumi, H., Imamura, T., Takano, H., Ise, T, Uchiumi, T., Kuwano, M. and Kohno, K.

    Oncogene   19 ( 54 )   6194 - 6202   2000.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/sj.onc.1204029

  • Human placental transport of vinblastine, vincristine, digoxin and progesterone: contribution of P-glycoprotein. Reviewed International journal

    Ushigome, F., Takanaga, H., Matsuo, H., Yanai, S., Tsukimori, K., Nakano, H., Uchiumi, T., Nakamura, T., Kuwano, M., Ohtani, H., and Sawada, Y.

    Eur. J. Pharmacol   408 ( 1 )   1 - 10   2000.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0014-2999(00)00743-3

  • Direct involvement of the Y-box binding protein YB-1 in genotoxic stress-induced activation of the human multidrug resistance 1 gene. Reviewed International journal

    Ohga,T., Uchiumi,T., Makino, Y., Koike,K., Wada,M., Kuwano, M. and Kohno,K.

    J. Biol. Chem.   273 ( 11 )   5997 - 6000   1999.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.273.11.5997

  • Enhanced coexpression of YB-1 and DNA topoisomerase IIαgenes in human colorectal carcinomas. Reviewed International journal

    83 ( 6 )   732 - 737   1999.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/(SICI)1097-0215(19991210)83:6<732::AID-IJC6>3.3.CO;2-R

  • Effect of multidrug resistance-reversing agents on transporting activity of human canalicular multispecific organic anion transporter. Invited Reviewed International journal

    Chen, Z., Kawabe, T., Ono, M., Aoki, S., Sumizawa, T., Furukawa, T., Uchiumi, T., Wada, M., Kuwano, M. and Akiyama, S.

    Molec. Pharmacology   56 ( 6 )   1219 - 1228   1999.1

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  • Transcription factor Y box-binding protein-1 binds preferentially to cisplatin-modified DNA and interacts with proliferating cell nuclear antigen. Reviewed International journal

    Ise, T., Nagatani, G., Imamura, T., Kato, K., Takano, H., Nomoto, M., Izumi, H., Ohmori, H., Okamoto, T., Ohga, T., Uchiumi, T., Kuwano, M. and Kohno, K.

    Cancer Res   59 ( 2 )   342 - 346   1999.1

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  • Genomic structure of the canalicular multispecitic organic anion transporter (cMOAT) gene and mutations in the ATP binding cassette region in Dubin-Johnson syndrome. Reviewed International journal

    Toh, S., Wada, M., Uchiumi, T., Inokuchi, A., Makino, Y., Horie, H., Adachi, Y., Sakisaka, S. and Kuwano, M.

    Am. J. Human Genet.   64 ( 3 )   739 - 746   1999.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1086/302292

  • Glutathione homeostasis in human hepatic cells: overexpression of γ-glutamylcysteine synthetase gene in cell lines resistant to buthionine sulfoximine, an inhibitor of glutathione synthesis.

    246 ( 2 )   398 - 403   1999.1

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  • Genomic organization of the human Y-box protein (YB-1) gene. Invited Reviewed International journal

    Toh,S., Nakamura,T., Ohga, T., Koike,K., Uchiumi,T., Wada,M., Kuwano,M. and Kohno,K.

    Gene   206 ( 1 )   93 - 97   1998.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0378-1119(97)00570-2

  • Localization of 67 exons on YAC contigs spanning 1.5 megabases around the multidrug resistance gene region of human chromosome 7q21.1. Reviewed International journal

    Torigoe, K., Harada, T., Kusaba, H., Uchiumi, T., Kohno, K., E.D.Green, S. W.Scherer, L-C.Tsui, D.Schlessinger, Kuwano,M. and Wada, M.

    Genomics   49 ( 1 )   14 - 22   1998.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1006/geno.1997.5200

  • Mutation in the canalicular multispecific organic anion transporter (cMOAT) gene, a novel ABC transporter, in patients with hyperbilirubinemia II/Dubin-Johnson syndrome. Reviewed International journal

    Wada,M., Toh, S., Taniguchi, K., Uchiumi,T. , Kohno,K., Yoshida, I., Kimura,A., Sakisaka, S., Adachi, Y. and Kuwano,M.

    Human Molec. Genet.   7 ( 2 )   203 - 207   1998.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/hmg/7.2.203

  • The role of an inverted CCAAT element in transcriptional activation of the human DNA topoisomeraseIIα gene in response to heat shock. Reviewed International journal

    273 ( 17 )   10550 - 10555   1998.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1074/jbc.273.17.10550

  • Nuclear expression of YB-1 protein correlates with P-glycoprotein expression in human osteosarcoma. Reviewed International journal

    Oda,Y., Sakamoto,A., Shinohara,N., Ohga,T., Uchiumi,T., Kohno,K., Tsuneyoshi,M., Kuwano,M. and Iwamoto,Y.

    Clin Cancer Res   4 ( 9 )   2273 - 2277   1998.1

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Books

  • 抗がん剤感受性とDubin-Johnson症候群

    内海健、和田守正、桑野信彦( Role: Joint author)

    学会出版センター  2005.5 

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    Responsible for pages:135-158頁   Language:Japanese   Book type:General book, introductory book for general audience

  • Canalicular multispecific organic anion transporter, ABCC2 MEMBRANE TRANSPORT DISEASES -Molecular basis of inherited transport defects-, edited by S. Brauner and C. A. Wagner

    Wada, M., Uchiumi,T. and Kuwano, M.:( Role: Joint author)

    NY. Kluwer Academic/Plenum Publishers  2003.5 

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    Responsible for pages:p263-289,   Language:English   Book type:Scholarly book

  • MRP2/cMOATの生理機能と変異・遺伝子多型 薬物動態・作用と遺伝子多型-薬物治療の患者個別化を目指した21世紀の新展開

    和田守正、内海健、桑野信彦( Role: Joint author)

    医薬ジャーナル社  2001.5 

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    Responsible for pages:276-285頁   Language:Japanese   Book type:General book, introductory book for general audience

  • 応用編, 治療応用に向けて, アポトーシスと疾患-癌編, 西條長宏編,

    内海健、五島大祐、桑野信彦( Role: Joint author)

    医薬ジャーナル社  1999.5 

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    Responsible for pages:150-158頁   Language:Japanese   Book type:General book, introductory book for general audience

Presentations

  • ミトコンドリアタンパクp32の脳特異的ノックアウトマウスの分子基盤と新規診断マーカーの探索

    内海 健

    第55回 日本臨床化学会  2015.10 

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    Event date: 2015.10 - 2015.11

    Language:Japanese  

    Venue:大阪   Country:Japan  

  • ミトコンドリア蛋白質p32のRAS依存性発ガン形質転換への関与

    内海 健

    第74回日本癌学会  2015.10 

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    Event date: 2015.10

    Language:Japanese  

    Venue:名古屋   Country:Japan  

  • 心筋特異的p32ノックアウトマウスは拡張型心筋症を発症するがmtUPR, autophagyを誘導し生存する

    内海 健, 八木美佳子, KANG DONGCHON

    第14回 日本ミトコンドリア学会  2014.12 

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    Event date: 2014.12

    Language:Japanese  

    Venue:福岡   Country:Japan  

  • ミトコンドリア蛋白質p32のRAS依存性発ガン形質転換への関与 Invited

    内海 健

    第61回 日本臨床検査医学会  2014.11 

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    Event date: 2014.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:福岡   Country:Japan  

  • 前立腺がんにおけるmtCK(mitochondria creatine kinase)発現様式とメタボローム

    内海 健, KANG DONGCHON

    第46回 日本臨床検査自動化学会  2014.10 

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    Event date: 2014.10

    Language:Japanese  

    Venue:神戸   Country:Japan  

  • Cardiomyocyte Specific Deletion of p32/C1qbp Causes Mitochondrial Cardiomyopathy International conference

    EuroMit  2014.6 

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    Event date: 2014.6

    Language:English  

    Country:Finland  

  • p32/gC1qR is indispensable for fetal development and mitochondrial translation: importance of its RNA binding ability Invited International conference

    2013.11 

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    Event date: 2013.11

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • ERAL1 is localized in mitochondria matrix and knockdown of ERAL1 leads to cell cycle arrest and growth retardation Invited

    Takeshi Uchiumi , Dongchon Kang

    2008.12 

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    Event date: 2008.12

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • ZNF143 interacts with p73 and involves in cisplatin resistance International conference

    Takeshi Uchiumi, Tetsuro Wakasugi, Hiroto Izumi, Kimitoshi Kohno

    0th IUBMB International Congress of Biochemistry and Molecular Biology  2006.6 

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    Country:Japan  

  • ミトコンドリア-リソソーム機能連関と疾患 Invited

    内海 健

    日本ミトコンドリア学会  2021.12 

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    Event date: 2021.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:東京 学習院大学   Country:Japan  

  • ミトコンドリア機能低下とリソソーム機能の新規連関

    内海健 康東天

    第66回 日本臨床検査医学会  2019.11 

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    Event date: 2019.11

    Language:Japanese  

    Country:Japan  

  • Mitochondrial translational impairment suppresses lysosomal function due to reduced NAD synthesis Invited International conference

    Takeshi Uchiumi Mikako Yagi Dongchon Kang

    2019.10 

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    Event date: 2019.10

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • 外部環境に応じたがん幹細胞様変化と抗生剤治療の可能性 Invited

    内海健

    第7回 癌と代謝研究会  2019.8 

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    Event date: 2019.8

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:仙台   Country:Japan  

  • 前立腺がん細胞株は 培養条件により癌幹細胞様変化及び可塑性を示す

    内海健

    第22回がん分子標的治療研究会  2018.5 

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    Event date: 2018.5

    Language:Japanese  

    Venue:東京   Country:Japan  

  • 外部環境による癌幹細胞様変化と抗生剤の有効性

    内海健

    第6回 癌と代謝研究会  2018.5 

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    Event date: 2018.5

    Language:Japanese  

    Venue:奄美大島   Country:Japan  

  • ミトコンドリアシャペロン蛋白p32の変異解析と心筋特異的p32KOマウス機能解析

    内海 健 八木美佳子 康東天

    第17回日本ミトコンドリア学会  2017.11 

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    Event date: 2017.11

    Language:Japanese  

    Venue:京都   Country:Japan  

  • 前立腺がん細胞株はsphere assayにより癌幹細胞様変化をきたしドキシサイクリンの有効性の検証

    内海健 康東天

    第64回 日本臨床検査医学学術集会  2017.11 

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    Event date: 2017.11

    Language:Japanese  

    Venue:京都   Country:Japan  

  • がん幹細胞は環境に応じた可塑性を示し抗生剤によるがん治療の分子機序

    内海健 康東天

    第49回日本臨床検査自動化学会  2017.9 

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    Event date: 2017.9

    Language:Japanese  

    Venue:横浜   Country:Japan  

  • Cardiomyocyte-specific loss of mitochondrial p32/C1qbp causes cardiomyopathy and activates stress responses

    Takeshi Uchiumi, Mikako Yagi, Dongchon Kang

    The 16th Conference of Japanese Society of Mitochondrial Research and Medicine J-mit  2016.10 

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    Event date: 2016.10 - 2016.11

    Language:Japanese  

    Country:Japan  

  • Doxycycline targets mitochondria of cancer stem like cell and causes apoptosis through ER stress

    2016.10 

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    Event date: 2016.10

    Language:Japanese  

    Country:Japan  

  • 変異型Ras依存的ガンは血清除去によりがん幹細胞様変化を示す

    内海健

    第20回 日本癌分子標的治療学会  2016.5 

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    Event date: 2016.5 - 2016.6

    Language:Japanese  

    Venue:別府   Country:Japan  

  • Cancer stem cell like phenotype induced by serum starvation in Eas transformed MEF cells due to Nitric oxide

    The 9th international conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide  2016.6 

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    Event date: 2016.5

    Language:Japanese  

    Country:Japan  

  • 白質脳症を示す神経特異的C1qbp/p32ノックアウトマウスの遺伝子発現、メタボローム解析

    内海 健

    分子生物学会  2015.12 

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    Event date: 2015.12

    Language:Japanese  

    Venue:神戸   Country:Japan  

  • 変異型Ras依存的ガンは血清除去によりミトコンドリア機能が変化する

    内海 健

    臨床検査医学会  2015.11 

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    Event date: 2015.11

    Language:Japanese  

    Venue:岐阜   Country:Japan  

  • Ras依存的発がんにおけるミトコンドリア形態と呼吸能の変化

    内海 健, Dongchon Kang

    第3回がんと代謝研究会  2015.7 

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    Event date: 2015.7

    Language:Japanese  

    Venue:金沢   Country:Japan  

  • 神経特異的p32ノックアウトマウスはオリゴデンドロサイト分化異常によって白質脳症になる

    八木美佳子, 内海 健, KANG DONGCHON

    第14回 日本ミトコンドリア学会  2014.12 

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    Event date: 2014.12

    Language:Japanese  

    Venue:福岡   Country:Japan  

  • Neuron-specific disruption of p32 gene in mouse causes selective loss of oligodendroglia cells leading to vacuolar degeneration in mid-brain International conference

    EuroMit  2014.6 

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    Event date: 2014.6

    Language:English  

    Country:Finland  

  • The mtCK expression is down-regulated in prostate cancer progression and correlated with mitochondrial OXPHOS function

    2013.10 

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    Event date: 2013.10

    Language:Japanese  

    Country:Japan  

  • ミトコンドリア呼吸鎖複合体タンパクをコードするmRNAの局在と結合蛋白

    内海 健

    日本ミトコンドリア学会  2012.12 

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    Event date: 2012.12

    Language:Japanese  

    Venue:筑波   Country:Japan  

  • ミトコンドリア翻訳調節因子p32の機能解析と細胞応答 Invited

    内海 健

    日本生化学会  2012.12 

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    Event date: 2012.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:福岡   Country:Japan  

  • 前立腺がんにおけるmtCK(mitochondria creatine kinase)の発現とその意義

    内海 健, 康 東天

    日本臨床検査医学会  2012.11 

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    Event date: 2012.11 - 2012.12

    Language:Japanese  

    Venue:京都   Country:Japan  

  • The expression of mtCK is down-regulated in prostate cancer progression and correlated with mitochondrial function

    2012.9 

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    Event date: 2012.9

    Language:Japanese  

    Country:Japan  

  • Embryonic lethality in mitochondrial p32 deficient, and the importance of p32 RNA binding for OXPHOS

    Takeshi Uchiumi, Mikako Yagi, Dongchon Kang

    2011.12 

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    Event date: 2011.12

    Country:Japan  

  • 前立腺がんで発現亢進するミトコンドリアRNA結合蛋白p32の機能解析

    内海健、康東天

    第58回 日本臨床検査医学会  2011.11 

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    Event date: 2011.11

    Venue:岡山   Country:Japan  

  • The expression of ubiquitous mitochondrial creatine kinase is downregulated in prostate cancer progression

    Takeshi Uchiumi, Rie Amamoto, Yoohyun Song, Yoshinao Oda, Masasumi Tsuneyoshi, Akira Yokomizo, Seiji Naito,

    2011.10 

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    Event date: 2011.10

    Country:Japan  

  • ミトコンドリアRNA結合蛋白p32の機能解析 Invited

    内海健

    第84回日本生化学会  2011.9 

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    Event date: 2011.9

    Presentation type:Symposium, workshop panel (public)  

    Venue:京都   Country:Japan  

  • Mitochondrial p32 is highly expressed in prostate cancer and is associated with shorter PSA relapse International conference

    Takeshi Uchiumi, Rie Amamoto, Dongchon Kang

    EuroMit 8 European Meeting on Mitochondrial Pathology,  2011.6 

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    Event date: 2011.6

    Country:Spain  

  • Mitochondrial p32 is highly expressed in prostate cancer and is associated with shorter PSA relapse time after prostatectomy International conference

    Takeshi Uchiumi, Rie Amamoto, Dongchon Kang

    The 10th Conference of Japanese Society of Mitochondrial reaserch and Medicine (J-Mit)  2010.12 

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    Event date: 2010.12

    Country:Japan  

  • ミトコンドリア蛋白p32/C1qBPは前立腺がんで高発現し予後マーカーとなりうる

    内海健、天本理恵、康東天

    第83回日本分子生物学会  2010.12 

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    Event date: 2010.12

    Venue:神戸ポートアイランド、神戸   Country:Japan  

  • Mitochondrial p32 is highly expressed in prostate cancer and is associated with PSA relapse time after prostatectomy

    Takeshi Uchiumi, Rie Amamoto, Yoshinao Oda, Masasumi Tsuneyoshi, Akira Yokomizo, Seiji Naito, Yoohyun Song

    2010.9 

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    Event date: 2010.9

    Country:Japan  

    Mitochondrial p32 is highly expressed in prostate cancer and is associated with PSA relapse time after prostatectomy

  • Role of ZNF143 in tumor growth through transcriptional regulation of DNA replication and cell cycle associated genes

    Masaki Akiyama, Hiroto Izumi, Eiji Kashiwagi, Yoshihiro Yasuniwa, Bin Han, Ying Wu, Takeshi Uchiumi, Tokuzou Arao, Kazuto Nishio, Kimitoshi Kohno

    2010.9 

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    Event date: 2010.9

    Country:Japan  

  • ミトコンドリア蛋白p32は前立腺がんの予後マーカーにな

    内海健、康東天

    第57回 日本臨床検査医学会  2010.9 

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    Event date: 2010.9

    Presentation type:Oral presentation (general)  

    Venue:第57回 日本臨床検査医学会   Country:Japan  

  • The knockdown of ERAL1 is involved in assembly of mitochondrial ribosome

    Takeshi Uchiumi, Dongchom Kang

    2009.12 

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    Event date: 2009.12

    Presentation type:Oral presentation (general)  

    Country:Japan  

  • The mitochondrial function and cell growth of mitochondrial translation related protein

    Takeshi Uchiumi , Dongchon Kang

    2009.12 

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    Event date: 2009.12

    Country:Japan  

  • Effects of overexpression of mitochondrial transcription factor A on mitochondrial genome in Drosophila melanogaster

    Chika Tsumita, takeshi Uchiumi, Dongchon Kang, Etsuko T. Matsuura

    2009.12 

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    Event date: 2009.12

    Country:Japan  

  • Mitochondrial translation related protein is involved in cancer cell growth and cell cycle

    Takeshi Uchiumi

    2009.10 

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    Event date: 2009.10

    Country:Japan  

  • The role of YB-1, a binding protein partner of NPM, in hematopoiesis and acute myeloid leukemia

    Yoko Ogasawara, Takuo Katsumoto, Takeshi Uchiumi, Kimitoshi Kohno, Issay Kitabayashi

    2009.10 

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    Event date: 2009.10

    Country:Japan  

  • ミトコンドリアDNA維持制御因子とがん Invited

    内海健、康東天

    第49回 日本臨床化学会  2009.9 

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    Event date: 2009.9

    Presentation type:Symposium, workshop panel (public)  

    Venue:長崎大学、長崎   Country:Japan  

  • がんにおけるミトコンドリア翻訳因子ERAL1の機能

    内海健、康東天

    第56回 日本臨床検査医学会  2009.8 

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    Event date: 2009.8

    Presentation type:Oral presentation (general)  

    Venue:札幌 、札幌コンベンションセンター   Country:Japan  

  • The knockdown of ERAL1 leads to dysfunction of mitochondria and cell cycle arrest

    Takeshi Uchiumi, Dongchom Kang

    2008.12 

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    Event date: 2008.12

    Country:Japan  

  • シスプラチン感受性規定因子とミトコンドリアDNA維持蛋白

    内海健、康東天

    第55回 日本臨床検査医学会  2008.11 

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    Event date: 2008.11

    Presentation type:Symposium, workshop panel (public)  

    Venue:名古屋   Country:Japan  

  • RNA binding protein ERAL1 is localized in mitochondrial matrix and ERAL1 siRNA leads to cell cycle arrest

    Takeshi Uchiumi

    2008.10 

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    Event date: 2008.10

    Country:Japan  

  • ERAL1 is localized in mitochondria matrix and knockdown of ERAL1 leads to cell cycle arrest and cell growth retardation International conference

    Takeshi Uchiumi , Dongchon Kang

    The 7th European Meeting on Mitochondrial Pathology  2008.7 

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    Event date: 2008.7

    Country:Sweden  

  • ヒトABCスーパーファミリーcMOAT/MRP2遺伝子の発現制御

    内海健、田中聡也、日下英司、和田守正、野本実、河野公俊、桑野信彦

    第58回日本癌学会総会  1999.8 

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    Presentation type:Oral presentation (general)  

    Venue:広島  

  • がんの血管新生と薬剤感受性の分子標的 Invited

    桑野信彦、和田守正、内海健、小野眞弓、河野公俊

    第58回日本癌学会総会  1999.9 

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    Presentation type:Symposium, workshop panel (public)  

    Venue:広島  

  • Effect of multidrug resistance reversing agents on the transporting activity of human canalicular multispecific organic anion Transporter (cMOAT). International conference

    Chen, Z. S., Kawabe, T., Ono, M., Sumizawa, T., Furukawa, T., Uchiumi, T., Wada, M., Kuwano, M., and Akiyama, S.

    AACR-NCI-EORTC Conferense (Molecular Target and Cancer Therapeutics)  1999.11 

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    Country:United States  

  • 転写因子Y-box結合蛋白(YB1)に結合する蛋白と発現様式

    内海健、中村崇規、岡本龍郎、芦塚慈美、河野公俊、桑野信彦

    第22回日本分子生物学会年会  1999.12 

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    Venue:福岡  

  • Chemotherapeutic implication of ATP binding cassette (ABC) transporter superfamily gene in cancer. Invited International conference

    Michihiko Kuwano, Takeshi Uchiumi, Morimasa Wada and Kimitoshi Kohno

    Eigth international Charles Heidelberger symposium on molecular carcinogenesis, molecular oncology, and cancer chemoyherapy  2000.3 

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    Presentation type:Symposium, workshop panel (public)  

    Country:United States  

  • Isolation of proteins closed associated with the Y-box binding protein (YB-1) and their express in malignant cancers. International conference

    Uchiumi, T., Nakamura, T., Ashizuka, M., Okamoto, T. and Kuwano, M.

    91th Annual Meeting of American Association for Cancer Research  2000.4 

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    Country:United States  

  • Direct interaction of Y-Box protein, YB-1, with p53 and regulation of human multidrug resistance 1 gene expression. International conference

    Okamoto, T., Takano, H., Imamura, T., Ise, T., Ohmori, H., Izumi, H., Uchiumi, T., Kuwano, M. and Kohno, K.

    91th Annual Meeting of American Association for Cancer Research  2000.4 

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    Country:United States  

  • A multidrug resistance protein MRP2: its possible association with drug responce to cisplatin in human colorectal cartinomas. International conference

    Hinoshita, E., Uchiumi, T., Taguchi, K., Kinugawa, N., Tsuneyoshi, M., Maehara, Y., Sugimati, K. and Kuwano, M.

    91th Annual Meeting of American Association for Cancer Research  2000.4 

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    Country:United States  

  • Expression and localization of MRP family genes in capillary-like structures of human brain endothelial cell. International conference

    Haga, S., Uchiumi, T., Hinoshita, E., Wada, M., Ikezaki, K., Furui, M. and Kuwano, M.

    91th Annual Meeting of American Association for Cancer Research  2000.4 

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    Country:United States  

  • ABCトランスポーター遺伝子ヒトMRP7の単離と発現

    内海健、中村崇規、和田守正、桑野信彦

    第59回日本癌学会総会  2000.10 

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    Venue:横浜  

  • Dubin-Johnson症候群の患者によるMRP2/cMOATのATP結合ドメインの変異:タンパク輸送系における欠損の証拠 International conference

    内海健

    第3回 FEBS Advanced Lecture Cours  2001.3 

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    Country:Austria  

  • Isolation of proteins associated with the Y-Box binding protein (YB-1) and their expression in malignant cancers International conference

    Ashizuka, M., Uchiumi, T., Nakamura, T., Okamoto, T., Fukuda, T., Kohno, K. and Kuwano, M.

    92th Annual Meeting of American Association for Cancer Research  2001.3 

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    Country:United States  

  • Retrovirus-associated activation of multidrug resistance (MDR) gene and MDR-related protein (MRP) family genes in hematopoietic malignancies. International conference

    Nagayama, J., Hinoshita, E., Iino, M., Kiue, A., Oka, M., Uchiumi, T., Wada, M. and Kuwano, M.

    91th Annual Meeting of American Association for Cancer Research  2001.4 

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    Country:United States  

  • ABCトランスポーター遺伝子ヒトMRP3の基質特異性と腫瘍での発現

    内海健、和田守正、桑野信彦

    第4回がん分子標的治療研究会総会  2001.6 

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    Presentation type:Oral presentation (general)  

    Venue:名古屋  

  • ヒト大腸癌の分化とABCトランスポーターMRP2遺伝子の発現変化と機序

    内海健、日下英司、田口健一、中村崇規、前原喜彦、和田守正、恒吉正澄、杉町圭藏、桑野信彦

    第60回日本癌学会総会  2001.9 

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    Venue:横浜  

  • The expression of human ABC superfamily MRP2 in association with differentiation in human colorectal carcinoma-experiment and clinical Approach. International conference

    Uchiumi, T., Hinoshita, E., Taguchi, K., Inokuchi, A., Nakamura, T., Wada, M. and Kuwano, M.

    92th Annual Meeting of American Association for Cancer Research  2002.3 

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    Country:United States  

  • YB-1(Y-box結合タンパク)による翻訳制御

    内海健、福田隆男、芦塚慈、中村崇規、桑野信彦

    第6回がん分子標的治療研究会総会  2002.6 

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    Presentation type:Oral presentation (general)  

    Venue:札幌  

  • Dubin-Johnson syndrome as a genetic disorder of a ABC transporter, MRP2/cMOAT. Invited International conference

    Wada, M., Uchiumi, T., Kuwano, M.

    4th HUGO Pacific Meeting and 5th Asia-Pacific Conference on HumaGenetics(Symposium)  2002.12 

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    Presentation type:Symposium, workshop panel (public)  

    Country:Thailand  

  • Traffiking and functional defects by mutations in MRP2 with Dubin-Johnson syndrome and the regulation of MRP3 expression Invited

    Uchiumi, T.

    Synposium on Priority Area"ABC protein"  2003.1 

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    Presentation type:Symposium, workshop panel (public)  

    Country:Japan  

  • ABCトランスポーター遺伝子MRP2の発現調節と疾病 Invited

    内海健

    第20回生物薬剤学研究会  2003.6 

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    Presentation type:Oral presentation (general)  

    Venue:熊本  

  • ABCトランスポーター遺伝子MRP2の炎症性サイトカインによる発現調節

    内海健、久枝啓史、和田守正、桑野信彦

    第7回がん分子標的治療研究会総会  2003.6 

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    Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  • MRP2の遺伝疾病と発現調節 Invited

    内海健

    公開シンポジウム「ABCタンパク質:生体防御と膜脂質ダイナミクス」  2003.8 

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    Presentation type:Oral presentation (general)  

    Venue:東京  

  • YB-1(Y-box結合タンパク)による翻訳制御 Invited

    内海健、桑野信彦

    第75回日本生化学大会  2003.10 

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    Presentation type:Symposium, workshop panel (public)  

    Venue:京都  

  • YB-1(Y-box 結合タンパク)による翻訳制御

    内海健、芦塚慈美、福田隆男、中村崇規、桑野信彦

    第25回日本分子生物学会年会  2003.12 

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    Presentation type:Oral presentation (general)  

    Venue:横浜  

  • YB-1(Y-box結合タンパク)とB23との結合様式

    内海健、福田隆男、中村崇規、桑野信彦

    第26回日本分子生物学会  2003.12 

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    Venue:神戸  

  • 抗がん剤感受性とDubin Johnson 症候群 Invited

    内海 健  

    公開シンポジウム「ABC蛋白の多機能性と生命維持機構」(ABC 2004 in AKITA)  2004.3 

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    Presentation type:Symposium, workshop panel (public)  

    Venue:秋田   Country:Japan  

  • Analysis of YB-1 Null Mutant Mouse Invited International conference

    Takeshi Uchiumi

    The 21st COE Program Kurume University Graduate School of Medicine  2005.11 

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    Country:Japan  

  • 時計遺伝子Clock及びATF4転写システムと多剤耐性

    五十嵐友紀、和泉弘人、吉田毅、新名一郎、若杉哲郎、宮本直哉、内海健、河野公俊

    第10回がん分子標的研究会  2006.6 

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    Venue:東京   Country:Japan  

  • RNA結合蛋白YB-1の機能と細胞増殖能,

    内海健、フォトバティアバス、續 輝久、和泉弘人、桑野信彦、河野公俊

    日本癌学会  2006.9 

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    Venue:横浜   Country:Japan  

  • RNA結合蛋白YB1の癌における機能と自己抗体

    内海健、康東天

    日本臨床検査医学会  2007.10 

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    Venue:大阪   Country:Japan  

  • Expression of cMOAT/MRP3 and cisplatin resistance in human glioma cell. International conference

    Haga,S., Uchiumi,T., Hinoshita,E., Wada,M., Ikezaki,K., Fukui,M. and Kuwano,M.

    90th Annual Meeting of American Association for Cancer Research  1999.4 

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    Country:United States  

  • Molecular mechanism of up-regulation of γ-glutamycysteine synthetase gene in hepatic cancer cells during exposure to an inhibitor of glutathione synthesis International conference

    1998.3 

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    Venue:New Orleans   Country:United States  

  • Nuclear translocation of the Y-box binding protein and human MDR1 gene activation International conference

    Uchiumi,T., Koike,K., Ohga,T., Toh,S., Wada,M., Kohno,K. and Kuwano,M.

    89th Annual Meeting of American Association for Cancer Research  1998.3 

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    Presentation type:Symposium, workshop panel (public)  

    Country:United States  

  • 5' CpG island demethylation and chromatin structure are associated with transcriptional activation of human multidrug resistance 1 (MDR1) gene in human cancer cells and hematopoietic neoplasia International conference

    Nakayama,M., Kusaba,H., Nagayama,J., Kohno,K., Komatsu,H., Ueda,R., Kozuru,M., Uchiumi,T., Kuwano,M. and Wada,M.

    89th Annual Meeting of American Association for Cancer Research  1998.3 

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    Country:United States  

  • The role of an inverted CCAAT element in the human DNA topoisomerase IIαgene activation in response to heat shock International conference

    1998.3 

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    Venue:New Orleans   Country:United States  

  • ヒト多剤耐性MDR1遺伝子の発現誘導と転写因子MDR-NNF1/YB-1活性化の分子機序

    内海健、大賀丈史、河野公俊、桑野信彦

    第2回がん分子標的治療研究会総会  1998.6 

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    Presentation type:Oral presentation (general)  

    Venue:東京  

  • ヒトABCスーパーファミリーcMOAT遺伝子群による抗がん剤感受性と排出の制御 Invited

    内海健、和田守正、桑野信彦

    第71回日本生化学大会  1998.10 

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    Presentation type:Symposium, workshop panel (public)  

    Venue:名古屋  

  • Transfection of human cMOAT/MRP2 cDNA induces altered sensitivity to anticancer agents and membrane transport of glutathione conjugates. International conference

    Kawabe,T., Wada,M., Nakamura,T., Uchiumi,T., Ono,M., Chen Z-s., Akiyama, S-i.,Kuwano,M.

    90th Annual Meeting of American Association for Cancer Research  1999.4 

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    Country:United States  

  • 電極の違いが心電図検査の精度に与える影響の検討

    横山 麗子, 川久保 芳文, 鶴本 雅信, 島崎 拓則, 堀田 多恵子, 後藤 和人, 内海 健, 康 東天, 赤司 浩一

    日本臨床検査医学会誌  2022.10  (一社)日本臨床検査医学会

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    Language:Japanese  

  • 近未来の臨床検査の情報共有と患者還元・社会貢献。その期待と課題 HL7-FHIRの導入に必要なJLAC10/11の整備 円滑な検査部運用のための提案

    堀田 多恵子, 清水 一範, 山田 修, 内山田 健次, 宮下 弘信, 片岡 浩巳, 内海 健, 松下 一之

    日本臨床検査医学会誌  2023.10  (一社)日本臨床検査医学会

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  • 血栓性素因スクリーニングの臨床的意義 新生児血栓症の全国調査からの洞察

    江上 直樹, 落合 正行, 市山 正子, 井上 普介, 園田 素史, 石村 匡崇, 堀田 多恵子, 内海 健, 康 東天, 大賀 正一

    日本産婦人科・新生児血液学会誌  2022.5  日本産婦人科・新生児血液学会

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  • 臨床検査のミッション データ利活用のための標準コード(JLAC)のミッション

    堀田 多恵子, 清水 一範, 山田 修, 内山田 健次, 宮下 弘信, 片岡 浩巳, 内海 健, 松下 一之

    日本臨床検査医学会誌  2023.10  (一社)日本臨床検査医学会

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    Language:Japanese  

  • 糖飢餓におけるferropotosisを介したリソソーム機能障害と防御機構

    三木 健嗣, 八木 美佳子, 康 東天, 國崎 祐哉, 吉本 幸司, 内海 健

    日本生化学会大会プログラム・講演要旨集  2023.10  (公社)日本生化学会

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    Language:Japanese  

  • 疾患病態におけるミトコンドリア機能 ミトコンドリア翻訳障害と糖飢餓はリソソームにフェロトーシスを引き起こす

    内海 健

    日本生化学会大会プログラム・講演要旨集  2023.10  (公社)日本生化学会

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  • 小児血栓止血学の診療update 新生児から成人までに発症する特発性血栓症の診療ガイドライン(仮)の策定に向けて

    落合 正行, 石村 匡崇, 山村 健一郎, 内海 健, 西久保 敏也, 野上 恵嗣, 石黒 精, 末延 聡一, 大賀 正一

    日本小児血液・がん学会雑誌  2022.10  (一社)日本小児血液・がん学会

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  • 全身麻酔下歯科治療のための血液検査結果を活用した小児の臨床参考範囲の設定

    横山 麗子, 横山 武志, 三木 洋一郎, 羽野 和宏, 大島 優, 一杉 岳, 内海 健, 國崎 祐哉

    日本臨床検査医学会誌  2023.10  (一社)日本臨床検査医学会

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  • 九州大学病院における再活性化B型肝炎対策に関する取り組み

    横山 麗子, 吉田 実, 内海 健, 國崎 祐哉, 後 信

    日本臨床検査医学会誌  2023.10  (一社)日本臨床検査医学会

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  • 九州地区における最先端の研究 ミトコンドリア関連疾患モデルマウスの分子機能解析とミトコンドリア遺伝子検査

    内海 健

    日本臨床検査医学会誌  2023.10  (一社)日本臨床検査医学会

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  • ミトコンドリアがつなぐ多彩な疾患研究最前線 ミトコンドリア機能障害による心不全の病態解析と治療戦略

    八木 美佳子, 内海 健, 康 東天

    日本生化学会大会プログラム・講演要旨集  2022.11  (公社)日本生化学会

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  • ビタミンK欠乏性出血症に対するビタミンK2シロップ予防投与後の凝固系検査 3回法と13回法の比較

    高橋 大二郎, 江上 直樹, 落合 正行, 堀田 多恵子, 菅 秀太郎, 石村 匡崇, 川口 千晴, 内海 健, 西久保 敏也, 野上 恵嗣, 後藤 啓, 大賀 正一

    日本産婦人科・新生児血液学会誌  2024.5  日本産婦人科・新生児血液学会

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  • SARS-CoV-2変異株解析において判定に苦慮した一症例

    松本 信也, 植柳 泰, 藤島 章義, 横山 麗子, 堀田 多恵子, 内海 健, 國崎 祐哉

    日本臨床検査医学会誌  2023.10  (一社)日本臨床検査医学会

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  • Real-time PCRを用いてLarge deletion領域を特定できたアンチトロンビン欠乏症の一症例

    松本 信也, 植柳 泰, 堀田 多恵子, 坂井 淳彦, 内海 健, 康 東天

    日本血栓止血学会誌  2022.5  (一社)日本血栓止血学会

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  • In vitroにおけるマイクロパーティクル放出とmiRNA発現の変化に関する基礎的検討

    龍 美玖璃, 塩津 弘倫, 内海 健

    医療検査と自動化  2022.8  (一社)日本医療検査科学会

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  • Digital PCRを用いたcell-free DNAによる筋層浸潤膀胱癌の病性評価(Monitoring of cell-free DNA with digital PCR in muscle invasive bladder cancer.)

    塚原 茂大, 塩田 真己, 内海 健, 松元 崇, 永川 祥平, 兒玉 啓輔, 江藤 正俊

    日本癌学会総会記事  2022.9  (一社)日本癌学会

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MISC

  • ミトコンドリアDNA維持制御因子とがん

    内海 健

    臨床化学   2010.9

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  • 【代謝】代謝と臓器・疾患 ミトコンドリア機能異常と老化機構

    内海 健

    生体の科学   74 ( 5 )   416 - 417   2023.10   ISSN:0370-9531

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    Language:Japanese   Publisher:(公財)金原一郎記念医学医療振興財団  

    <文献概要>老化はミトコンドリア異常が引き金になること,またミトコンドリアは細胞内恒常性維持のため様々なオルガネラと相互作用することが知られている。老化の進展はミトコンドリアの機能異常が他のオルガネラ,特にリソソームの機能異常によって増悪化を引き起こすことを見いだした。特にHIF1α-NAD+合成系酵素を介してリソソーム機能障害を引き起こす新分子機序について紹介する。

  • 栓友病と遺伝性血栓性素因 母子を守る個別医療へ

    大賀 正一, 江上 直樹, 堀田 多恵子, 内海 健, 落合 正行, 石村 匡崇

    臨床血液   64 ( 9 )   1131 - 1136   2023.9   ISSN:0485-1439

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    Language:Japanese   Publisher:(一社)日本血液学会-東京事務局  

    小児血栓症患者における遺伝素因の報告が増えている。小児期は年齢ごとに発症リスクが異なり,遺伝素因の寄与度も様々である。20歳までに発症する血栓症のうち遺伝素因が関与するものを"早発型遺伝性血栓症(early-onset thrombophilia,EOT)"として登録し,網羅的文献検索と併せて本邦患者の遺伝的背景と臨床経過を明らかにした。患者の60%以上をプロテインC(PC)欠乏症が占め,その半分以上が片アレル変異の保有者だった。年齢とともにプロテインS(PS)およびアンチトロンビン欠乏症が増加して主体となった。6~8歳の発症はなかった。日本人高頻度・低リスクバリアントのPC-TottoriとPS-Tokushimaも確認されたが,前者は重症PC欠乏症の発症に寄与していなかった。解析した32家系中de novo発症は1家系のみで,周産期の同時発症母子例が3家系あった。PC欠乏症を標的に適切なEOTスクリーニングを行って周産期血栓症から母子を守ることが期待される。(著者抄録)

  • 血栓性素因スクリーニングの臨床的意義 新生児血栓症の全国調査からの洞察

    江上 直樹, 落合 正行, 市山 正子, 井上 普介, 園田 素史, 石村 匡崇, 堀田 多恵子, 内海 健, 康 東天, 大賀 正一

    日本産婦人科・新生児血液学会誌   32 ( 2 )   51 - 58   2023.3   ISSN:0916-8796

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    新生児期の血栓症は希少な事例と考えられてきたが、医療の高度化と疾患認識の広がりにより報告数が増加している。さらに、日本人小児の血栓性素因における遺伝性プロテインC欠乏症の重要性が示されてきた。今回私たちは新生児血栓症の全国調査を行い、遺伝性プロテインC欠乏症の遺伝学的効果と、遺伝学的検査の悉皆性を示すことができた。今後は調査対象を小児から成人に達するまでに拡大し、小児患者とその家族を血栓症から守る個別化医療の確立を目指す。(著者抄録)

  • 【ミトコンドリア 疾患治療の新時代 オルガネラ動態を紐解き異常ミトコンドリアの標的分子を狙う!】(第2章)各種疾患・病態とのかかわり ミトコンドリアによる生体恒常性の維持 ミトコンドリア翻訳機構とその破綻による疾患

    八木 美佳子, 内海 健

    実験医学   41 ( 5 )   734 - 740   2023.3   ISSN:0288-5514

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    Language:Japanese   Publisher:(株)羊土社  

    ミトコンドリア翻訳機構が破綻すると細胞内恒常性が維持できず種々の疾患が引き起こされる.さらにミトコンドリアはオルガネラ間クロストークにも関与するため,ミトコンドリア機能障害は,ミトコンドリアだけでなくER,リソソーム,核内転写因子など多くのオルガネラ機能まで破綻させる可能性がある.ミトコンドリア翻訳制御因子p32を心臓,脳,樹状細胞など組織特異的にノックアウトしそれぞれの生理学的機能を確認すると,組織ごとに病状は異なり障害を及ぼす経路も異なるものであった.p32の組織によって異なる分子機序について紹介する.(著者抄録)

  • 慢性心不全モデルマウスの病態解析と治療戦略

    平井 遥, 八木 美佳子, 都 由羅, 瀬戸山 大樹, 堀田 多恵子, 内海 健

    臨床化学   52 ( 1 )   47 - 50   2023.1   ISSN:0370-5633

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  • マイクロベシクルが含有する核酸とミトコンドリアの解析

    上田 沙央理, 内海 健, 松本 信也, 堀田 多恵子, 康 東天

    臨床化学   51 ( 1 )   41 - 45   2022.1   ISSN:0370-5633

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    マイクロベシクルは細胞膜の出芽により様々な細胞型から生成する小胞で,ヒト体液中に多く存在することからバイオマーカーとして注目を集めている.多くの核酸やミトコンドリアなどのオルガネラを含有し,細胞間で受け渡すことでシグナル伝達にも寄与している.また,ミトコンドリアはATP産生などの役割を果たす一方で,mtDNAは体内で炎症を引き起こす分子としても機能する.そして,マイクロベシクルとmtDNAはともに炎症の際に血液中で数が増加することが報告されている.このような背景を踏まえて,我々は血液中のマイクロベシクルとミトコンドリアに注目し,組織特異的な炎症のバイオマーカー確立を目指して解析を行っている.本稿では,疾患群との比較を見据えて作成した健常人プロファイルについて報告する.(著者抄録)

  • シスプラチン感受性規定因子とミトコンドリアDNA維持機構

    内海 健、康東天

    臨床病理   2009.5

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  • 抗がん剤の標的としてのミトコンドリアゲノム

    河野公俊、和泉弘人、内海健

    細胞   2006.5

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  • Transcription factors and drug resistance.

    Kohno,K., Uchiumi,T., Niina,I., Wakasugi,T., Igarashi,T., Momii,Y., Yoshida,T., Matsuo,K., Miyamoto,N. and Izumi,H.

    Eur. J. Cancer,   2005.5

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  • The role of nuclear Y-box binding protein 1 as a global marker in drug resistance

    Kuwano,M., Oda,Y., Izumi,H.,Yang,S.J., Uchiumi,T.,Iwamoto,Y., Toi,M., Fujii,T., Yamana,H., Kinoshita,H., Kamura,T., Tsuneyoshi,M., Yasumoto,K. and Kohno,K.

    Mol. Cancer Ther   2004.5

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  • The basic and clinical implication of ABC transporters, YB-1 and angiogenesis in human malignancies.

    Kuwano,M., Uchiumi,T., Hayakawa,H., Ono,M., Wada,M., Izumi,H. and Kohno,K.

    Cancer Sci.   2003.5

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  • MRP2/cMOATの生理機能と変異・遺伝子多型

    和田守正、内海健、桑野信彦

    医薬ジャーナル社   2001.1

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  • アポトーシスと疾患ー癌編

    内海健、五島大祐、桑野信彦

    医薬ジャーナル社   1999.1

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  • ABCトランスポーターと体質性黄疸、肝内胆汁うっ滞(特集:ATP結合カセット(ABC)タンパク質の分子医学)

    和田守正、藤賢史、内海健、桑野信彦

    最新医学   1999.1

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  • ABCトランスポーター(基礎・Topics)

    桑野信彦、内海健、和田守正、河野公俊

    医薬ジャーナル社   1999.1

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  • P糖タンパク質/MDR1とcMOATの薬剤耐性への関与

    内海健、日下英司、芳賀整、和田守正、桑野信彦

    最新医学   1999.1

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  • ABCトランスポーターの病態

    和田守正、内海健、中村崇規、桑野信彦

    生体の科学   1999.1

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  • YB-1 と MDR1 遺伝子の転写調節

    大賀丈史、内海健、桑野信彦、河野公俊

    1998.1

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  • 抗癌剤耐性機構

    和田守正、内海健、桑野信彦

    血液・腫瘍科   1998.1

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  • 薬剤排出ポンプ-ABCトランスポータースーパーファミリー

    内海健、和田守正、桑野信彦

    血液・免疫・腫瘍   1998.1

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Professional Memberships

  • 日本生化学会

  • 分子標的研究会

  • 日本分子生物学会

  • Japanease Cancer Association

  • American Association of Cancer Research

  • 日本臨床検査学会

  • 日本臨床化学会

  • 日本ミトコンドリア学会

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Academic Activities

  • 科学研究費審査委員

    Role(s): Review, evaluation

    2020.6 - 2021.3

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    Type:Scientific advice/Review 

  • 科学研究費審査委員

    Role(s): Review, evaluation

    文部科学省  2019.4 - 2020.3

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    Type:Scientific advice/Review 

  • 科学研究費審査委員

    Role(s): Review, evaluation

    文部科学省  2018.5 - 2019.3

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    Type:Scientific advice/Review 

  • Screening of academic papers

    Role(s): Peer review

    2018

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    Type:Peer review 

    Number of peer-reviewed articles in foreign language journals:2

  • 座長(Chairmanship)

    臨床検査医学会  ( 岐阜 ) 2015.11

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    第55回 日本臨床化学会  ( 大阪 ) 2015.10 - 2015.11

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    第14回 日本ミトコンドリア学会  ( 福岡 ) 2014.12

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    第61回 日本臨床検査医学会  ( 福岡 ) 2014.11

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    第34回日本分子生物学会  ( 横浜 ) 2011.12

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    第58回 日本臨床検査医学会  ( 岡山 ) 2011.11

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  • 座長(Chairmanship)

    第49回 日本臨床化学会  ( 長崎大学、長崎 ) 2009.9

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  • 座長(Chairmanship)

    第8回日本ミトコンドリア学会  ( 東京 ) 2008.12

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Research Projects

  • 検査実用化を目指したシーズ探索 及び有用性の検討

    2023.6 - 2024.4

    共同研究

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    Authorship:Principal investigator  Grant type:Other funds from industry-academia collaboration

  • クロレラとミトコンドリア機能解析

    2023.6 - 2024.4

    受託研究

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    Authorship:Principal investigator  Grant type:Other funds from industry-academia collaboration

  • 肝臓組織特異的発現遺伝子のサイレント変異で翻訳が停止する新規分子機序

    Grant number:23K18217  2023 - 2024

    日本学術振興会  科学研究費助成事業  挑戦的研究(萌芽)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 検査実用化を目指したシーズ探索 及び有用性の検討

    2022.6

    共同研究

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    Authorship:Principal investigator  Grant type:Other funds from industry-academia collaboration

  • クロレラのミトコンドリア機能及び解析

    2022.6

    共同研究

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    Authorship:Principal investigator  Grant type:Other funds from industry-academia collaboration

  • ミトコンドリア機能異常による老化疾患の分子病態の解明とNMNによる治療効果

    Grant number:22H03537  2022 - 2024

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • ミトコンドリア病態解析に基づく臓器特異的ミトコンドリア異常診断法の開発

    2020 - 2022

    日本学術振興会  科学研究費助成事業  基盤研究(A)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 先天性門脈体循環シャント症候群を引き起こす遺伝的要因の解明

    2018 - 2020

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 網羅的代謝変化によるミトコンドリア異常疾患の病態解析とバイオマーカー探索

    2018 - 2020

    日本学術振興会  科学研究費助成事業  基盤研究(A)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • ミトコンドリア異常と代償的葉酸代謝亢進の分子基盤と予防栄養の探索

    2018 - 2020

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 抗老化に繋がるミトコンドリアストレス応答機構解明と診断分子の探索、有効性

    Grant number:15H04764  2015 - 2017

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • ミトコンドリアRNAシャペロンp32の分子基盤と病態解析

    Grant number:24590387  2012 - 2014

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • ミトコンドリア蛋白p32の前立腺がんにおける発現・機能解析

    2010

    臨床検査医学研究振興基金研究奨励

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    Authorship:Principal investigator  Grant type:Contract research

  • ミトコンドリア蛋白p32の意義と診断法の開発

    2010

    黒住医学研究振興財団研究助成金

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    Authorship:Principal investigator  Grant type:Contract research

  • ミトコンドリア内外の協調的翻訳機構の分子基盤

    Grant number:21590337  2009 - 2011

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 増殖性疾患でのCSD蛋白の発現と分子間ネットワーク

    Grant number:18590307  2006 - 2007

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 概日時計破綻、酸化ストレスによる生体反応ホメオスターシスの分子基盤

    2006

    産業医科大学 重点研究

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    Authorship:Principal investigator  Grant type:Contract research

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Educational Activities

  • I teach first, second, and third year health science courses as part of my undergraduate education.
    Clinical Chemistry I, Clinical Chemistry II, and Introduction to Laboratory Management,
    The fourth-year undergraduate students are instructed in basic research as their graduation research.
    As for graduate education, 2 students in the doctoral course of the School of Medicine, 1 student in the doctoral course of the School of Health Sciences, and 6 students in the master's course are engaged in research activities.

Class subject

  • 検査技術科学特別研究(内海教授)

    2024.4 - 2025.3   Full year

  • 人体の構造と機能Ⅰ

    2024.4 - 2024.9   First semester

  • 検査管理総論

    2024.4 - 2024.9   First semester

  • 国際社会とチーム医療

    2024.4 - 2024.9   First semester

  • ヘルスサイエンス論

    2024.4 - 2024.9   First semester

  • 生体情報機能検査学演習

    2024.4 - 2024.9   First semester

  • 生体情報解析学Ⅰ

    2024.4 - 2024.9   First semester

  • International Society and Multidisciplinary Care

    2024.4 - 2024.9   First semester

  • 臨床検査総論

    2024.4 - 2024.6   Spring quarter

  • 一般検査学実習

    2023.12 - 2024.2   Winter quarter

  • 生物化学分析検査学特論

    2023.10 - 2024.3   Second semester

  • 生化学・臨床化学実習

    2023.10 - 2024.3   Second semester

  • 生体情報機能検査学

    2023.10 - 2024.3   Second semester

  • 生体情報解析学Ⅱ

    2023.10 - 2024.3   Second semester

  • 一般検査学Ⅰ

    2023.10 - 2023.12   Fall quarter

  • 臨床検査学概論Ⅱ

    2023.10 - 2023.12   Fall quarter

  • 国際プレゼンテーション(内海教授)

    2023.4 - 2024.3   Full year

  • 保健学特別研究(内海教授)

    2023.4 - 2024.3   Full year

  • 検査技術科学特別研究(内海教授)

    2023.4 - 2024.3   Full year

  • 人体の構造と機能Ⅰ

    2023.4 - 2023.9   First semester

  • 検査管理総論

    2023.4 - 2023.9   First semester

  • 臨床化学Ⅱ

    2023.4 - 2023.9   First semester

  • 先進臨床検査特論

    2023.4 - 2023.9   First semester

  • 国際社会とチーム医療

    2023.4 - 2023.9   First semester

  • ヘルスサイエンス論

    2023.4 - 2023.9   First semester

  • 生体情報機能検査学演習

    2023.4 - 2023.9   First semester

  • 生体情報解析学Ⅰ

    2023.4 - 2023.9   First semester

  • International Society and Multidisciplinary Care

    2023.4 - 2023.9   First semester

  • Health Science

    2023.4 - 2023.9   First semester

  • 臨床検査総論

    2023.4 - 2023.6   Spring quarter

  • 臨床検査学概論Ⅰ

    2023.4 - 2023.6   Spring quarter

  • 臨床化学Ⅰ

    2022.10 - 2023.3   Second semester

  • 生化学・臨床化学実習

    2022.10 - 2023.3   Second semester

  • 生物化学分析検査学特論

    2022.10 - 2023.3   Second semester

  • 生体情報解析学Ⅱ

    2022.10 - 2023.3   Second semester

  • 生体情報機能検査学

    2022.10 - 2023.3   Second semester

  • 臨床検査学概論Ⅱ

    2022.10 - 2022.12   Fall quarter

  • 国際プレゼンテーション(内海教授)

    2022.4 - 2023.3   Full year

  • 保健学特別研究(内海教授)

    2022.4 - 2023.3   Full year

  • 検査技術科学特別研究(内海教授)

    2022.4 - 2023.3   Full year

  • 人体の構造と機能Ⅰ

    2022.4 - 2022.9   First semester

  • 検査管理総論

    2022.4 - 2022.9   First semester

  • 臨床化学Ⅱ

    2022.4 - 2022.9   First semester

  • 先進臨床検査特論

    2022.4 - 2022.9   First semester

  • 国際社会とチーム医療

    2022.4 - 2022.9   First semester

  • ヘルスサイエンス論

    2022.4 - 2022.9   First semester

  • 生体情報機能検査学演習

    2022.4 - 2022.9   First semester

  • 生体情報解析学Ⅰ

    2022.4 - 2022.9   First semester

  • International Society and Multidisciplinary Care

    2022.4 - 2022.9   First semester

  • Health Science

    2022.4 - 2022.9   First semester

  • 臨床検査学概論Ⅰ

    2022.4 - 2022.6   Spring quarter

  • 卒業研究

    2021.4 - 2022.3   Full year

  • 国際プレゼンテーション(内海教授)

    2021.4 - 2022.3   Full year

  • 保健学特別研究(内海教授)

    2021.4 - 2022.3   Full year

  • 検査技術科学特別研究(内海教授)

    2021.4 - 2022.3   Full year

  • 国際社会とチーム医療

    2021.4 - 2021.9   First semester

  • ヘルスサイエンス論

    2021.4 - 2021.9   First semester

  • 生体情報機能検査学演習

    2021.4 - 2021.9   First semester

  • 生体情報解析学Ⅰ

    2021.4 - 2021.9   First semester

  • Health Science

    2021.4 - 2021.9   First semester

  • International Society and Multidisciplinary Care

    2021.4 - 2021.9   First semester

  • 検査技術科学特別研究(内海教授)

    2020.4 - 2021.3   Full year

  • 保健学特別研究(内海教授)

    2020.4 - 2021.3   Full year

  • 国際プレゼンテーション(内海教授)

    2020.4 - 2021.3   Full year

  • Health Science

    2020.4 - 2020.9   First semester

  • 生体情報機能検査学演習

    2020.4 - 2020.9   First semester

  • 生体情報解析学Ⅰ

    2020.4 - 2020.9   First semester

  • International Society and Multidisciplinary Care

    2020.4 - 2020.9   First semester

  • Biological Sciences and Technology Ⅰ

    2020.4 - 2020.9   First semester

  • ヘルスサイエンス論

    2020.4 - 2020.9   First semester

  • 国際社会とチーム医療

    2020.4 - 2020.9   First semester

  • 生化学・臨床化学実習

    2019.10 - 2020.3   Second semester

  • 臨床化学Ⅰ

    2019.10 - 2020.3   Second semester

  • 検査技術科学特別研究(内海教授)

    2019.4 - 2020.3   Full year

  • 保健学特別研究(内海教授)

    2019.4 - 2020.3   Full year

  • 臨床化学II

    2019.4 - 2019.9   First semester

  • 生体情報解析学Ⅰ

    2019.4 - 2019.9   First semester

  • 人体の構造と機能Ⅰ

    2019.4 - 2019.9   First semester

  • 検査管理総論

    2019.4 - 2019.9   First semester

  • 臨床化学Ⅱ

    2019.4 - 2019.9   First semester

  • 先進臨床検査特論

    2019.4 - 2019.9   First semester

  • 国際社会とチーム医療

    2019.4 - 2019.9   First semester

  • ヘルスサイエンス論

    2019.4 - 2019.9   First semester

  • 生体情報機能検査学演習

    2019.4 - 2019.9   First semester

  • 先進検査学総論

    2019.4 - 2019.9   First semester

  • 管理総論

    2019.4 - 2019.9   First semester

  • 生命科学科

    2018.10 - 2019.3   Second semester

  • 医学科

    2018.4 - 2018.9   First semester

  • 生命科学科

    2017.10 - 2018.3   Second semester

  • 生命科学科

    2016.10 - 2017.3   Second semester

  • 生命科学科

    2015.10 - 2016.3   Second semester

  • 生命科学

    2014.10 - 2015.3   Second semester

  • 医学生物学概論

    2013.10 - 2014.3   Second semester

  • 生命科学科

    2013.10 - 2014.3   Second semester

  • 医学生物学概論

    2012.10 - 2013.3   Second semester

  • 生命科学科

    2012.10 - 2013.3   Second semester

  • 医学生物学概論

    2011.10 - 2012.3   Second semester

  • 生命科学科

    2010.10 - 2011.3   Second semester

  • 医学教育

    2010.4 - 2010.9   First semester

  • 総合生命科学セミナー

    2009.10 - 2010.3   Second semester

  • 臨床検査医学

    2008.4 - 2008.9   First semester

  • 臨床検査医学

    2007.4 - 2007.9   First semester

  • 臨床検査医学

    2007.4 - 2007.9   First semester

  • 生命基礎医学・生化学・分子医化学

    1998.4 - 1998.9   First semester

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FD Participation

  • 2023.9   Role:Participation   Title:九州大学医学部保健学科 FD

    Organizer:Undergraduate school department

  • 2022.9   Role:Participation   Title:保健学FD

    Organizer:Undergraduate school department

  • 2019.6   Role:Participation   Title:保健学科FD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

Other educational activity and Special note

  • 2024  Class Teacher 

  • 2023  Class Teacher 

  • 2022  Class Teacher 

  • 2021  Class Teacher 

  • 2020  Class Teacher 

  • 2019  Class Teacher 

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Specialized clinical area

  • Biological Sciences / Medicine, Dentistry and Pharmacy / Borderline Medicine / Pathophysiology

Clinician qualification

  • Specialist

    Japanese Society of Laboratory Medicine(JSLM)

Year of medical license acquisition

  • 1988