Updated on 2024/10/17

Information

 

写真a

 
WADA NAOHISA
 
Organization
Faculty of Dental Science Department of Dental Science Professor
Research Center for Education in Health Care System (Concurrent)
Kyushu University Hospital Clinical Education Center(Concurrent)
Kyushu University Hospital Health Networking Center(Concurrent)
Abolition organization Comprehensive Dentistry(Concurrent)
School of Dentistry Department of Dentistry(Concurrent)
Graduate School of Dental Science (Concurrent)
Graduate School of Dental Science Department of Dental Science(Concurrent)
Title
Professor
Tel
0926426490
Profile
research: To achieve regeneration of periodontal ligament, I study about chracterization of periodontal ligament and searching the specific factor for periodontal ligament.
Homepage
External link

Degree

  • Ph.D ( Kyushu University )

Research History

  • 2007/9月-2010/3月 Postdoctorlal Research Officer; Colgate Australian Clinical Dental Research Centre, School of Dentistry, the University of Adelaide   

Research Interests・Research Keywords

  • Research theme: Establishing novel method to regenerate dentin-dental pulp complex using stem cells, signal molecules and scaffolds.

    Keyword: dental pulp, dentin, stem cell, tissure engineering

    Research period: 2013.4

  • Research theme: Identification of novel growth factor and extracellular matrix protein which are specific in periodontal ligament stem cell population

    Keyword: Periodontal ligament stem cell

    Research period: 2010.4

  • Research theme: Reprogramming of human gingival fibroblasts and periodontal ligament cells to pluripotency with defined factors

    Keyword: gingival cell, periodontal ligament cell, iPS

    Research period: 2008.4 - 2009.3

  • Research theme: Immunosuppressive mechanisms of allogeneic periodontal ligament stem cells on T-lymphocytes

    Keyword: periodontal ligament stem cell, T-lymophocyte, allogeneic transplantation

    Research period: 2007.9 - 2010.3

  • Research theme: Identification of novel factors to induce periodontal tissue regeneration

    Keyword: periodontal ligament

    Research period: 2006.4

  • Research theme: Establishment and characterisation of human periodontal ligament cell clone and epithelial cell clone derived from human epithelial rests of Malassez

    Keyword: periodontal ligament, cell clone

    Research period: 2003.4 - 2006.3

  • Research theme: Lipopolysaccharide stimulates expression of osteoprotegerin and receptor activator of NF-kappa B ligand in periodontal ligament fibroblasts through the induction of interleukin-1 beta and tumor necrosis factor-alpha

    Keyword: periodontal ligament, osteoclast, LPS

    Research period: 2001.4 - 2003.3

  • Research theme: The study of the mechanism(s) and the factor for periodontal ligament cells to regulate the osteoclast differentiation and function

    Keyword: periodontal ligament, osteoclast

    Research period: 1998.4 - 2002.3

  • Research theme: Apoptosis of epithelial cells proliferated in human periapical lesions

    Keyword: periapical granuloma, periapical cyst, apoptosis

    Research period: 1997.4 - 1998.3

Awards

  • 日本歯科保存学会奨励賞

    2003.6   日本歯科保存学会  

Papers

  • Actin alpha 2, smooth muscle, a transforming growth factor-β1-induced factor, regulates collagen production in human periodontal ligament cells via Smad2/3 pathway. Reviewed International journal

    Naati Fakatava, Hiromi Mitarai, Asuka Yuda, Akira Haraguchi, Hiroko Wada, Daigaku Hasegawa, Hidefumi Maeda, Naohisa Wada

    Journal of dental sciences   18 ( 2 )   567 - 576   2023.4   ISSN:1991-7902 eISSN:2213-8862

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Journal of Dental Sciences  

    BACKGROUND/PURPOSE: Actin alpha 2, smooth muscle (ACTA2) is an actin isoform that forms the cytoskeleton. Actin plays a crucial role in numerous cellular functions. ACTA2 is a marker of functional periodontal ligament (PDL) fibroblasts and is upregulated by transforming growth factor-β1 (TGF-β1); however, the underlying function of ACTA2 in PDL tissue is unknown. We aimed to examine the localization and potential function of ACTA2 in PDL tissues and cells. MATERIALS AND METHODS: RNA expression was determined using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and quantitative RT-PCR. Protein expression was determined using immunofluorescence staining and Western blot analysis. Soluble and insoluble collagen production was examined using the Sircol collagen assay and picrosirius red staining, respectively. Small interfering RNA (siRNA) was used for knockdown assay to examine the effect of ACTA2 in human PDL cells. RESULTS: ACTA2 expression was observed in human primary PDL cells and PDL cell line (2-23 cells). TGF-β1 upregulated ACTA2, collagen type Ⅰ alpha1 chain (COL1A1), periostin (POSTN), and fibrillin-Ⅰ(FBN1) expression and soluble and insoluble collagen production in 2-23 cells. However, ACTA2 depletion by siRNA strongly suppressed PDL-related gene expression and collagen production compared with those of TGF-β1-stimulated control cells. Furthermore, ACTA2 knockdown significantly suppressed the phosphorylation of Smad2 and Smad3. CONCLUSION: ACTA2 plays a crucial role in PDL-related marker expression and collagen production via Smad2/3 phosphorylation. Our findings might contribute to the development of novel and effective periodontal therapies.

    DOI: 10.1016/j.jds.2022.08.030

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  • Wnt/β-catenin signaling, which is activated in odontomas, reduces Sema3A expression to regulate odontogenic epithelial cell proliferation and tooth germ development Reviewed

    Shinsuke Fujii, Kengo Nagata, Shinji Matsumoto, Kenichi Kouhashi, Akira Kikuchi, Yoshinao Oda, Tamotsu Kiyoshima, Naohisa Wada

    Scientific reports   9 ( 1 )   2019.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41598-019-39686-1

  • 骨組織上に播種した歯髄幹細胞は歯根膜関連遺伝子を発現する-接触する基質の硬さが細胞分化に及ぼす影響- Reviewed

    吉田晋一郎, 和田尚久, 長谷川大学, 御手洗裕美, 有馬麻衣, 友清淳, 濱野さゆり, 杉井英樹, 前田英史

    日本歯科保存学雑誌   61 ( 6 )   343 - 353   2018.12

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

  • Wnt5a suppresses osteoblastic differentiation of human periodontal ligament stem cell-like cells via Ror2/JNK signaling Reviewed

    Daigaku Hasegawa, Naohisa Wada, Shinichiro Yoshida, Hiromi Mitarai, Mai Arima, Atsushi Tomokiyo, Sayuri Hamano, Hideki Sugii, Hidefumi Maeda

    Journal of Cellular Physiology   233 ( 2 )   1752 - 1762   2018.2

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    DOI: 10.1002/jcp.26086

  • Transgelin mediates transforming growth factor-β1-induced proliferation of human periodontal ligament cells Reviewed

    H. Mitarai, Naohisa Wada, Daigaku Hasegawa, Shinichiro Yoshida, M. Sonoda, Atsushi Tomokiyo, Sayuri Hamano, S. Serita, H. Mizumachi, Hidefumi Maeda

    Journal of Periodontal Research   52 ( 6 )   984 - 993   2017.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/jre.12466

  • Semaphorin 3A Induces Odontoblastic Phenotype in Dental Pulp Stem Cells. Reviewed International journal

    Shinichiro Yoshida, Naohisa Wada, Daigaku Hasegawa, H. Miyaji, H. Mitarai, Atsushi Tomokiyo, Sayuri Hamano, Hidefumi Maeda

    J Dent Res   95 ( 11 )   1282 - 1290   2016.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/0022034516653085

  • Wnt5a Induces Collagen Production by Human Periodontal Ligament Cells through TGFβ1-mediated Upregulation of Periostin Expression. Reviewed International journal

    Daigaku Hasegawa, Naohisa Wada, Hidefumi Maeda, S.Yoshida, H. Mitarai, Atsushi Tomokiyo, Monnouchi Satoshi, S. Hamano, A. Yuda, Akifumi Akamine

    J Cell Physiol   230 ( 11 )   2647 - 2660   2015.11

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  • Microbial community in persistent apical periodontitis: a 16S rRNA gene clone library analysis. Reviewed International journal

    Zakaria MN, Toru Takeshita, Yukie Shibata, Hidefumi Maeda, Naohisa Wada, Akifumi Akamine, Yoshihisa Yamashita

    Int Endod J   2014.8

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  • Periodontal Ligament Derived Stem Cells Exhibit the Capacity for Long-Term Survival, Self-Renewal and Regeneration of Multiple Tissue Types in Vivo. Reviewed International journal

    Menicanin D, Mrozik M, Naohisa Wada, Marino V, Shi S, Bartold PM, Gronthos S.

    Stem Cells Dev   23 ( 9 )   1001 - 1011   2014.5

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  • Semaphorin 3A induces mesenchymal stem-like properties in human periodontal ligament cells. Reviewed International journal

    Naohisa Wada, Hidefumi Maeda, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Yoshida D, Tomokiyo A, Monnouchi Satoshi, Akifumi Akamine

    Stem Cells Dev   2014.2

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  • Regeneration of periodontal tissues using allogeneic periodontal ligament stem cells in an ovine model. Reviewed International journal

    Mrozik K, Naohisa Wada, Marino V, Richter W, Shi S, Wheeler DL, Gronthos S, Bartold PM

    Regen Med   8 ( 6 )   711 - 723   2013.6

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  • Induced pluripotent stem cell lines derived from human gingival fibroblasts and periodontal ligament fibroblasts. Reviewed International journal

    Wada N, Wang B, Lin NH, Laslett AL, Gronthos S, Bartold PM

    J Periodontal Res   46 ( 4 )   2011.5

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  • Human foreskin fibroblasts exert immunomodulatory properties by a different mechanism to bone marrow mesenchymal stem cells Reviewed International journal

    Wada N, Bartold PM, Gronthos S

    Stem Cells Dev   20 ( 4 )   2010.9

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  • Immunomodulatory properties of human periodontal ligament stem cells Reviewed International journal

    Wada N, Menicanin D, Shi S, Bartold PM, Gronthos S

    J Cell Physiol   219   2009.1

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  • Development of a multipotent clonal human periodontal ligament cell line. Differentiation Reviewed International journal

    Tomokiyo A, Maeda H, Fujii S, Wada N, Shima K, Akamine A

    Differentiation   76 ( 4 )   2008.4

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  • Investigating a clonal human periodontal ligament progenitor/stem cell line in vitro and in vivo Reviewed International journal

    Fujii S, Maeda H, Wada N, Tomokiyo A, Saito M, Akamine A.

    J Cell Physiol   215 ( 3 )   2008.3

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  • Establishing and characterizing human periodontal ligament fibroblasts immortalized by SV40T-antigen and hTERT gene transfer. Reviewed International journal

    Fujii S, Maeda H, Wada N, Kano Y, Akamine A

    Cell and Tissue Research   2006.4

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  • Identification of genes differentially expressed in osteoclast-like cells. Reviewed International journal

    Nomiyama H, Egami K, Wada N, Tou K, Horiuchi M, Matsusaki H, Miura R, Yoshie O, Kukita T

    Journal of Interferon and Cytokine Research   25 ( 4 )   227 - 231   2005.1

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    DOI: 10.1089/jir.2005.25.227

  • RANKL-induced DC-STAMP is essential for osteoclastogenesis. Reviewed International journal

    Kukita T, Wada N, Kukita A, Kakimoto T, Sandra F, Toh K, Nagata K, Iijima T, Horiuchi M, Matsusaki H, Hieshima K, Yoshie O, Nomiyama H.

    Journal of Experimental Medicine   200 ( 7 )   941 - 946   2004.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1084/jem.20040518

  • Lipopolysaccharide stimulates expression of osteoprotegerin and receptor activator of NF-kappa B ligand in periodontal ligament fibroblasts through the induction of interleukin-1 beta and tumor necrosis factor-alpha. Reviewed International journal

    Wada N, Maeda H, Yoshimine Y, Akamine A

    Bone   35 ( 3 )   629 - 635   2004.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bone.2004.04.023

  • Apoptosis of epithelial cells proliferated in human periapical lesions. Reviewed

    Wada N, Maeda H, Nakamuta H, Akamine A

    日本歯科保存学会誌   2003.1

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

  • Periodontal ligament cells secrete the factor that inhibits osteoclastic differentiation and function: the factor is osteoprotegerin / osteoclastogenesis inhibitory factor. Reviewed International journal

    Wada N, Maeda H, Tanabe K, Tsuda E, Yano K, Nakamuta H, Akamine A

    Journal of Periodontal Research   36 ( 1 )   56 - 63   2001.1

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    DOI: 10.1034/j.1600-0765.2001.00604.x

  • In vitro evaluation of the antimicrobial properties of terpinen-4-ol on apical periodontitis-associated bacteria. Reviewed International journal

    Kamiya H, Haraguchi A, Mitarai H, Yuda A, Wada H, Wang S, Ran Z, Sun W, Wada N

    J Infect Chemother.   30 ( 4 )   306 - 314   2024.4

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    DOI: doi: 10.1016/j.jiac.2023.10.021.

  • Serum lipopolysaccharide-binding protein levels and the incidence ofmetabolic sy ndrome in a general Japanese population: the Hisayama Study. Reviewed International journal

    Tomooka S, Oishi E, Asada M, Sakata S, Hata J, Chen S, Honda T, Suzuki K, Watanabe H, Murayama N, Wada N, Kitazono T, Ninomiya T.

    J Epidemiol.   34 ( 1 )   1 - 7   2024.4

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    DOI: doi.org/10.2188/jea.JE20220232

  • In vitro evaluation of the antimicrobial properties of terpinen-4-ol on apical periodontitis-associated bacteria

    Kamiya, H; Haraguchi, A; Mitarai, H; Yuda, A; Wada, H; Wang, SX; Ran, ZQ; Sun, WH; Wada, N

    JOURNAL OF INFECTION AND CHEMOTHERAPY   30 ( 4 )   306 - 314   2024.4   ISSN:1341-321X eISSN:1437-7780

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    Language:English   Publisher:Journal of Infection and Chemotherapy  

    Manuka oil and tea tree oil are essential oils with known antibacterial properties that are believed to be caused by one main component: terpinen-4-ol. Terpinen-4-ol has potent antibacterial activity against caries-related microorganisms. However, few studies have investigated the antimicrobial effects of terpinen-4-ol on bacteria in apical periodontitis. Thus, the objective of the present study was to evaluate the antibacterial and antibiofilm potential of terpinen-4-ol against Enterococcus faecalis, Porphyromonas gingivalis, Prevotella intermedia, and Fusobacterium nucleatum, which have all been detected in apical periodontitis. The minimum inhibitory and minimum bactericidal concentrations of terpinen-4-ol were determined to assess its activity against biofilms. The minimum inhibitory concentration of terpinen-4-ol was 0.25% against E. faecalis and F. nucleatum, 0.05% against P. gingivalis, and 0.1% against P. intermedia. The minimum bactericidal concentration of terpinen-4-ol was 1.0% against E. faecalis, 0.2% against P. gingivalis and P. intermedia, and 0.5% against F. nucleatum. In the biofilm evaluations, all terpinen-4-ol-treated bacteria had significant reductions in biofilm viability compared with controls in experiments assessing attachment inhibitory activity. Furthermore, structural alterations and decreased bacterial cell clumping were observed under scanning electron microscopy, and significantly decreased cell survival was noted using fluorescence microscopy. Together, these results suggest that terpinen-4-ol is a potential antibacterial agent with bactericidal properties, and can also act on established biofilms.

    DOI: 10.1016/j.jiac.2023.10.021

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  • In vitro evaluation of the antimicrobial properties of terpinen-4-ol on apical periodontitis-associated bacteria(タイトル和訳中)

    Kamiya Harunobu, Haraguchi Akira, Mitarai Hiromi, Yuda Asuka, Wada Hiroko, Wang Shuxin, Ran Ziqing, Sun Weihao, Wada Naohisa

    Journal of Infection and Chemotherapy   30 ( 4 )   306 - 314   2024.4   ISSN:1341-321X

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    Language:English   Publisher:エルゼビア・ジャパン(株)  

  • Possible association between oral health status and appetite loss in community-dwelling older adults. Reviewed International journal

    Inoue S, Suma S, Furuta M, Wada N, Yamashita Y

    Nurs Health Sci   26 ( 1 )   e13111   2024.3

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    DOI: doi.org/10.1111/nhs.13111

  • 血清リポ多糖結合蛋白濃度とメタボリックシンドローム発症との関連 久山町研究(Serum Lipopolysaccharide-binding Protein Levels and the Incidence of Metabolic Syndrome in a General Japanese Population: the Hisayama Study)

    Tomooka Shoko, Oishi Emi, Asada Masako, Sakata Satoko, Hata Jun, Chen Sanmei, Honda Takanori, Suzuki Kosuke, Watanabe Hiroshi, Murayama Norihito, Wada Naohisa, Kitazono Takanari, Ninomiya Toshiharu

    Journal of Epidemiology   34 ( 1-2 )   1 - 7   2024.2   ISSN:0917-5040

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    Language:English   Publisher:(一社)日本疫学会  

    <Highlight>●本研究は、血清リポ多糖結合蛋白(LBP)値とメタボリックシンドローム(MetS)発症との関連を検討した最初の前向きコホート研究である。●血清LBP値の上昇に伴いメタボリックシンドロームおよびその構成要素の発症リスクは上昇した。●媒介解析において、HOMA-IRと血清高感度CRPは血清LBPとMetS発症との関連に、部分的に介在していた。●血清LBP値とメタボリックシンドロームの発症リスクの関連はインスリン抵抗性と慢性炎症を介した機序が示唆された。(著者抄録)

  • 有床義歯に強い抵抗感のある患者に対し義歯再製作を試みた1症例

    久保 健太郎, 伊吹 禎一, 和田 尚久

    日本総合歯科学会雑誌   15   30 - 36   2023.10

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    患者が訴える義歯のトラブルのひとつに,異物感や嘔吐感のため義歯を口に入れておけないということがある。本稿では,歯の欠損部を放置する原因となった義歯の違和感について考察した1症例を報告する。症例は71歳女性。過去2回,部分床義歯(義歯)を製作したが,上顎は違和感,下顎は強い痛みのため義歯に対する抵抗感が特に強く,15年以上義歯の使用はなかった。咬合支持域が1か所しかなく,十分な栄養摂取と残存歯の維持のために義歯補綴が必須と思われた。患者が受容しやすいよう,比較的抵抗感の低かった上顎義歯を先に製作することにした。旧義歯の形態が舌感に強く影響していると思われたため,パラタルバーの位置と人工歯の排列に配慮して新義歯を製作した。飲み込みやすさは改善したが舌が義歯に当たる感触は変わらず,長時間使用できないとのことだった。そこで舌房を垂直的に拡大するため咬合を挙上すると舌感は改善し,継続的な義歯の使用が可能となった。上顎に続き製作した下顎の新義歯も話しにくく使えないとの訴えがあったが,人工歯と義歯床の舌側を切削すると改善した。本症例における患者が抱く義歯の違和感の原因は舌感であり,義歯の形態だけではなく,咬合高径も関与することが示された。また,舌房に影響する歯の位置的変化や咬合高径の低下が生じている可能性があるため,部分床義歯を必要とする歯列には慎重な観察が必要であることが示唆された。(著者抄録)

  • Prediction of dynamic allostery for the transmembrane domain of the sweet taste receptor subunit, TAS1R3

    Sanematsu Keisuke, Yamamoto Masato, Nagasato Yuki, Kawabata Yuko, Watanabe Yu, Iwata Shusuke, Takai Shingo, Toko Kiyoshi, Matsui Toshiro, Wada Naohisa, Shigemura Noriatsu

    Communications Biology   6   340   2023.4   eISSN:23993642

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    Language:English   Publisher:Springer  

    The sweet taste receptor plays an essential role as an energy sensor by detecting carbohydrates. However, the dynamic mechanisms of receptor activation remain unclear. Here, we describe the interactions between the transmembrane domain of the G protein-coupled sweet receptor subunit, TAS1R3, and allosteric modulators. Molecular dynamics simulations reproduced species-specific sensitivity to ligands. We found that a human-specific sweetener, cyclamate, interacted with the mouse receptor as a negative allosteric modulator. Agonist-induced allostery during receptor activation was found to destabilize the intracellular part of the receptor, which potentially interfaces with the Gα subunit, through ionic lock opening. A common human variant (R757C) of the TAS1R3 exhibited a reduced response to sweet taste, in support of our predictions. Furthermore, histidine residues in the binding site acted as pH-sensitive microswitches to modulate the sensitivity to saccharin. This study provides important insights that may facilitate the prediction of dynamic activation mechanisms for other G protein-coupled receptors.

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  • Prediction of dynamic allostery for the transmembrane domain of the sweet taste receptor subunit, TAS1R3. Reviewed International journal

    Keisuke Sanematsu, Masato Yamamoto, Yuki Nagasato, Yuko Kawabata, Yu Watanabe, Shusuke Iwata, Shingo Takai, Kiyoshi Toko, Toshiro Matsui, Naohisa Wada, Noriatsu Shigemura

    Communications biology   6 ( 1 )   340 - 340   2023.4   eISSN:2399-3642

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Communications Biology  

    The sweet taste receptor plays an essential role as an energy sensor by detecting carbohydrates. However, the dynamic mechanisms of receptor activation remain unclear. Here, we describe the interactions between the transmembrane domain of the G protein-coupled sweet receptor subunit, TAS1R3, and allosteric modulators. Molecular dynamics simulations reproduced species-specific sensitivity to ligands. We found that a human-specific sweetener, cyclamate, interacted with the mouse receptor as a negative allosteric modulator. Agonist-induced allostery during receptor activation was found to destabilize the intracellular part of the receptor, which potentially interfaces with the Gα subunit, through ionic lock opening. A common human variant (R757C) of the TAS1R3 exhibited a reduced response to sweet taste, in support of our predictions. Furthermore, histidine residues in the binding site acted as pH-sensitive microswitches to modulate the sensitivity to saccharin. This study provides important insights that may facilitate the prediction of dynamic activation mechanisms for other G protein-coupled receptors.

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  • 食道癌手術症例における入院前周術期支援の意義

    原武 直紀, 清松 丈浩, 淀川 千穂, 須古井 和美, 伊藤 心二, 和田 尚久, 水元 一博, 中川 尚志

    臨牀と研究   100 ( 3 )   368 - 372   2023.3   ISSN:0021-4965

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    食道癌手術症例における入院前周術期支援の介入の有無と術後合併症との関連について検討し、入院前周術期支援の有用性について検証した。2018年4月~2020年9月にかけて、食道癌手術症例120例を、入院前周術期支援を開始した2019年10月前後で入院時支援介入なし群75例と介入あり群45例に分類した。術後合併症は全体で51例(43%)に認められ、内容としては最多が肺炎で16例(13.9%)、嚥下障害・反回神経麻痺が14例(12.2%)、不整脈8例(7.0%)であった。介入なし群での合併症頻度は75例中37例(49%)であったのに対して、介入あり群で45例中14例(31%)と介入あり群の方が合併症の発生頻度が低かった。周術期支援による術後合併症の減少が示唆される結果が得られ、他癌腫への応用も視野に、周術期支援の充実を図ることが重要と考えられた。

  • Bisphosphonate affects the behavioral responses to HCl by disrupting farnesyl diphosphate synthase in mouse taste bud and tongue epithelial cells. Reviewed International journal

    Asami Oike, Shusuke Iwata, Ayaka Hirayama, Yurika Ono, Yuki Nagasato, Yuko Kawabata, Shingo Takai, Keisuke Sanematsu, Naohisa Wada, Noriatsu Shigemura

    Scientific reports   12 ( 1 )   21246 - 21246   2022.12   ISSN:2045-2322

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Scientific Reports  

    Little is known about the molecular mechanisms underlying drug-induced taste disorders, which can cause malnutrition and reduce quality of life. One of taste disorders is known adverse effects of bisphosphonates, which are administered as anti-osteoporotic drugs. Therefore, the present study evaluated the effects of risedronate (a bisphosphonate) on taste bud cells. Expression analyses revealed that farnesyl diphosphate synthase (FDPS, a key enzyme in the mevalonate pathway) was present in a subset of mouse taste bud and tongue epithelial cells, especially type III sour-sensitive taste cells. Other mevalonate pathway-associated molecules were also detected in mouse taste buds. Behavioral analyses revealed that mice administered risedronate exhibited a significantly enhanced aversion to HCl but not for other basic taste solutions, whereas the taste nerve responses were not affected by risedronate. Additionally, the taste buds of mice administered risedronate exhibited significantly lower mRNA expression of desmoglein-2, an integral component of desmosomes. Taken together, these findings suggest that risedronate may interact directly with FDPS to inhibit the mevalonate pathway in taste bud and tongue epithelial cells, thereby affecting the expression of desmoglein-2 related with epithelial barrier function, which may lead to alterations in behavioral responses to HCl via somatosensory nerves.

    DOI: 10.1038/s41598-022-25755-5

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  • Serum lipopolysaccharide-binding protein levels and the incidence of metabolic syndrome in a general Japanese population: the Hisayama Study. Reviewed

    Shoko Tomooka, Emi Oishi, Masako Asada, Satoko Sakata, Jun Hata, Sanmei Chen, Takanori Honda, Kosuke Suzuki, Hiroshi Watanabe, Norihito Murayama, Naohisa Wada, Takanari Kitazono, Toshiharu Ninomiya

    Journal of epidemiology   34 ( 1 )   1 - 7   2022.12   ISSN:09175040 eISSN:13499092

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    <p><b>Background:</b> The association between chronic lipopolysaccharide exposure and the development of metabolic syndrome (MetS) is unclear. In this study we examined the association between serum lipopolysaccharide-binding protein (LBP) levels, an indicator of lipopolysaccharide exposure, and the development of MetS in a general Japanese population.</p><p><b>Methods:</b> 1,869 community-dwelling Japanese individuals aged ≥40 years without MetS at baseline examination in 2002–2003 were followed up by repeated examination in 2007–2008. MetS was defined according to the Japanese criteria. Serum LBP levels were classified into quartiles (quartiles 1–4: 2.20–9.56, 9.57–10.78, 10.79–12.18, and 12.19–24.34 µg/mL, respectively). Odds ratios (ORs) for developing MetS were calculated using a logistic regression model.</p><p><b>Results:</b> At the follow-up survey, 159 participants had developed MetS. Higher serum LBP levels were associated with greater risk of developing MetS after multivariable adjustment for age, sex, smoking, drinking, and exercise habits (OR [95% confidence interval] for quartiles 1–4: 1.00 [reference], 2.92 [1.59–5.37], 3.48 [1.91–6.35], and 3.86 [2.12–7.03], respectively; <i>P</i> for trend <0.001). After additional adjustment for homeostasis model assessment of insulin resistance, this association was attenuated but remained significant (<i>P</i> for trend = 0.007). On the other hand, no significant association was observed after additional adjustment for serum high-sensitivity C-reactive protein (<i>P</i> for trend = 0.07).</p><p><b>Conclusion:</b> In the general Japanese population, our findings suggest that higher serum LBP levels are associated with elevated risk of developing MetS. Low-grade endotoxemia could play a role in the development of MetS through systemic chronic inflammation and insulin resistance.</p>

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  • 患者情報やコミュニケーション技法を積極的に活用し歯科治療が可能となった1症例

    信太 実有, 御手洗 裕美, 和田 尚久

    日本総合歯科学会雑誌   14   39 - 46   2022.10

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    定期的な口腔内清掃を希望する患者が来院するが,歯周疾患の治癒・病状安定を維持している患者が多い一方,病状が進行し予後不良の歯が出現しているものの,様々な理由で治療や抜歯を希望しない患者もいる。今回,患者情報を参考に,様々なコミュニケーション技法を応用して患者と治療方針を検討した。その結果,抜歯を含めた全顎的な治療に取り組むことができた症例を経験したため報告する。症例は76歳,男性。「右上の歯が痛い。」という主訴で来院した。患者は九州大学病院口腔総合診療科で全顎的治療を行った後にSupportive Periodontal Therapy(SPT)へ移行したが,その後約5年にわたり「簡単に掃除をやってほしい。」との訴えが続いた。そのため,定期的な口内法X線写真撮影や歯周基本検査を含む,十分なSPTを行えていなかった。そこで,過去の患者情報から患者の考え方を考察し,(1)目標設定理論を応用し,何か処置をする前には,処置内容,所要時間,その処置が必要な理由をその都度伝えること,(2)"But You Are Free"(BYAF) compliance-gaining techniqueを応用した質問形式を取ること,(3)"Self Persuasion"自主説得理論を応用した治療の提案を行うことのコミュニケーション技法を応用して治療を開始した。その結果,患者は現症や治療方法を十分理解・納得し,予後不良と判断した歯の抜歯と,上顎即時義歯を製作することができた。以上のことから,患者情報や様々なコミュニケーション技法を応用することで,治療へ移行ができる可能性があると考えられた。(著者抄録)

  • 咬合挙上を行い歯冠補綴空隙を確保した1症例

    与那嶺 亮, 伊吹 禎一, 和田 尚久

    日本総合歯科学会雑誌   14   30 - 38   2022.10

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    歯の欠損部が治療されずに長期間放置されていた結果,歯の挺出等によって歯列が乱れ,歯冠補綴を困難にすることがある。本稿では,両側臼歯部の補綴治療に際し歯冠補綴空隙の確保を咬合挙上で対応した1症例を報告する。症例は29歳女性,う蝕と欠損部の治療を主訴に当科を受診した。初診時,歯の挺出や傾斜のため咬合平面が乱れ,特に上顎左5は支台のまま対合歯と接触していた。まず欠損部に顎位の変更のないプロビジョナルレストレーション(PR)を装着した。そのPRを,咬合器上の顎模型で咬合高径を挙上し製作したPRと置換した。置換後の経過観察中,初診時の顎位からの側方移動や患者の不調の訴えはなかった。前方誘導も確保され,安定した咬合が得られたと判断し,その顎位で最終補綴装置の装着に至った。本症例では,歯冠補綴空隙の確保の手段のひとつとして,咬合挙上が有効であることが示唆され,治療計画立案時の咬合器上の顎模型の詳細な観察・分析が特に重要と考えられた。(著者抄録)

  • The Semaphorin 3A-AKT axis-mediated cell proliferation in salivary gland morphogenesis and adenoid cystic carcinoma pathogenesis Reviewed

    Shinsuke Fujii, Tatsufumi Fujimoto, Kana Hasegawa, Ryoko Nagano, Takuma Ishibashi, Kari J. Kurppa, Yurie Mikami, Megumi Kokura, Yudai Tajiri, Toshiro Kibe, Hiroko Wada, Naohisa Wada, Shosei Kishida, Yoshinori Higuchi, Tamotsu Kiyoshima

    Pathology Research and Practice   236   153991   2022.8   ISSN:0344-0338 eISSN:1618-0631

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    We recently demonstrated that Semaphorin 3 A (Sema3A), the expression of which is negatively regulated by Wnt/β-catenin signaling, promotes odontogenic epithelial cell proliferation, suggesting the involvement of Sema3A in tooth germ development. Salivary glands have a similar developmental process to tooth germ development, in which reciprocal interactions between the oral epithelium and adjacent mesenchyme proceeds via stimulation with several growth factors; however, the role of Sema3A in the development of salivary glands is unknown. There may thus be a common mechanism between epithelial morphogenesis and pathogenesis; however, the role of Sema3A in salivary gland tumors is also unclear. The current study investigated the involvement of Sema3A in submandibular gland (SMG) development and its expression in adenoid cystic carcinoma (ACC) specimens. Quantitative RT-PCR and immunohistochemical analyses revealed that Sema3A was expressed both in epithelium and in mesenchyme in the initial developmental stages of SMG and their expressions were decreased during the developmental processes. Loss-of-function experiments using an inhibitor revealed that Sema3A was required for AKT activation-mediated cellular growth and formation of cleft and bud in SMG rudiment culture. In addition, Wnt/β-catenin signaling decreased the Sema3A expression in the rudiment culture. ACC arising from salivary glands frequently exhibits malignant potential. Immunohistochemical analyses of tissue specimens obtained from 10 ACC patients showed that Sema3A was hardly observed in non-tumor regions but was strongly expressed in tumor lesions, especially in myoepithelial neoplastic cells, at high frequencies where phosphorylated AKT expression was frequently detected. These results suggest that the Sema3A-AKT axis promotes cell growth, thereby contributing to morphogenesis and pathogenesis, at least in ACC, of salivary glands.

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  • Relationship between motor dysfunction and chewing movement in patients with Parkinson’s disease: a transversal study. Reviewed International journal

    #Sano T, @Umemoto G, @Fujioka S, @Iwashita Y, @Dotsu Y, @Wada N, @Tsuboi Y

    Front Neurol   13   1062134   2022.6

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    DOI: doi.org/10.3389/fneur.2022.1062134

  • Effects of ultraviolet irradiation equipment on endodontic disease–related bacteria

    Akira Haraguchi, Shinichiro Yoshida, Masaaki Takeshita, Yasunori Sumi, Hiromi Mitarai, Asuka Yuda, Hiroko Wada, Fusanori Nishimura, Hidefumi Maeda, Naohisa Wada

    Lasers in Dental Science   6 ( 1 )   31 - 40   2022.3   eISSN:2367-2587

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    Introduction: Dental caries and apical periodontitis are ones of the most prevalent chronic diseases and involve infection by cariogenic and endodontic bacteria. It can be said that the most required to cure is disinfection. We hypothesized that NB-UVB could be used for intraoral disinfection without affecting cells, and that it could be used in combination with TiO2 to disinfect complex root canals. The objectives were to investigate the effects of UV on dental pulp cells and oral bacteria and to evaluate the enhancement effect of a photocatalyst on bactericidal effects of UV irradiation. Methods: UV irradiation devices of UVB (310, 285 nm) and UVC (265 nm) were prepared. Cell proliferation and cytotoxicity assays after UV irradiation were performed using human dental pulp cells. The antibacterial activity of UV irradiation was investigated in Streptococcus mutans, Staphylococcus aureus, Enterococcus faecalis, Actinomyces naeslundii, Porphyromonas gingivalis, and Fusobacterium nucleatum. A curved simulated root canal with plastic training block was used to evaluate the effect of combined UV and TiO2 treatment. Data were analyzed by one-way ANOVA (p < 0.05) followed by Tukey’s post hoc tests (p < 0.05). Results: Human dental pulp cell proliferation was decreased by 265 nm, 285 nm, and 310 nm UV irradiation, although 310 nm UV irradiation did not show cytotoxic effects on these cells. Oral bacterial growth was suppressed following UV irradiation at 285 nm and 265 nm. Viability of all bacteria significantly decreased with UV irradiation. In the curved simulated root canal, viability of E. faecalis in UV irradiation at 285 nm with long taper fibers was significantly decreased in the 300 s irradiation group. E. faecalis proliferation was inhibited by combined UV irradiation and TiO2 application with long taper fibers in the curved simulated root canal. Conclusions: The wavelength of UVB and UVC showed bactericidal effects on oral bacteria including caries-related bacteria and apical periodontitis–related bacteria while NB-UVB did not. UVB with longer fibers was more effective in disinfection on E. faecalis in curved simulated root canal, and the combined use of photocatalyst further improved the disinfection effect.

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  • Development of an artificial intelligence-based algorithm to classify images acquired with an intraoral scanner of individual molar teeth into three categories

    Nozomi Eto, Junichi Yamazoe, Akiko Tsuji, Naohisa Wada, Noriaki Ikeda

    PLoS ONE   17 ( 1 )   e0261870   2022.1   ISSN:1932-6203 eISSN:1932-6203

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    Background Forensic dentistry identifies deceased individuals by comparing postmortem dental charts, oral-cavity pictures and dental X-ray images with antemortem records. However, conventional forensic dentistry methods are time-consuming and thus unable to rapidly identify large numbers of victims following a large-scale disaster. Objective Our goal is to automate the dental filing process by using intraoral scanner images. In this study, we generated and evaluated an artificial intelligence-based algorithm that classified images of individual molar teeth into three categories: (1) full metallic crown (FMC); (2) partial metallic restoration (In); or (3) sound tooth, carious tooth or non-metallic restoration (CNMR). Methods A pre-trained model was created using oral-cavity pictures from patients. Then, the algorithm was generated through transfer learning and training with images acquired from cadavers by intraoral scanning. Cross-validation was performed to reduce bias. The ability of the model to classify molar teeth into the three categories (FMC, In or CNMR) was evaluated using four criteria: precision, recall, F-measure and overall accuracy. Results The average value (variance) was 0.952 (0.000140) for recall, 0.957 (0.0000614) for precision, 0.952 (0.000145) for F-measure, and 0.952 (0.000142) for overall accuracy when the algorithm was used to classify images of molar teeth acquired from cadavers by intraoral scanning. Conclusion We have created an artificial intelligence-based algorithm that analyzes images acquired with an intraoral scanner and classifies molar teeth into one of three types (FMC, In or CNMR) based on the presence/absence of metallic restorations. Furthermore, the accuracy of the algorithm reached about 95%. This algorithm was constructed as a first step toward the development of an automated system that generates dental charts from images acquired by an intraoral scanner. The availability of such a system would greatly increase the efficiency of personal identification in the event of a major disaster.

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  • Effects of ultraviolet irradiation equipment on endodontic disease–related bacteria. Reviewed International journal

    Haraguchi A, Yoshida S, Takeshita M, Sumi Y, Mitarai H, Yuda A, Wada H, Nishimura F, Maeda H, Wada N

    Lasers Dent Sci   6   31 - 40   2022.1

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    DOI: 10.1007/s41547-021-00145-8.

  • Incidence of postoperative pneumonia and oral microbiome for patients with cancer operation. Reviewed International journal

    Nomura Y, Inai Y, Shimpo Y, Okada A, Yamamoto Y, Sogabe K, Wada N, Hanada N

    Appl. Sci   12 ( 6 )   2920   2022.1   eISSN:2076-3417

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    Featured Application Increased Atopobium parvulum, Enterococcus faecalis, Fusobacterium nucleatum, and Porphyromonas gingivalis can be a high risk for the incidence of postoperative pneumonia. Postoperative pneumonia is a serious problem for patients and medical staff. In Japan, many hospitals introduced perioperative oral care management for the efficient use of medical resources. However, a high percentage of postoperative pneumonia still developed. Therefore, there is a need to identify the specific respiratory pathogens to predict the incidence of pneumonia The purpose of this study was to find out the candidate of bacterial species for the postoperative pneumonia. This study applied case-control study design for the patients who had a cancer operation with or without postoperative pneumonia. A total of 10 patients undergoing a cancer operation under general anesthesia participated in this study. The day before a cancer operation, preoperative oral care management was applied. Using the next generation sequence, oral microbiome of these patients was analyzed at the time of their first visit, the day before and after a cancer operation. Porphyromonas gingivalis and Fusobacterium nucleatum group can be a high risk at first visit. Atopobium parvulum and Enterococcus faecalis before a cancer operation can be a high risk. Poor oral hygiene increased the risk of incidence of postoperative pneumonia. Increased periodontal pathogens can be a high risk of the incidence of postoperative pneumonia. In addition, increased intestinal bacteria after oral care management can also be a high risk for the incidence of postoperative pneumonia.

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  • Development of an artificial intelligence-based algorithm to classify images acquired with an intraoral scanner of individual molar teeth into three categories. Reviewed International journal

    Eto N, Yamazoe J, Tsuji A, Wada N, Ikeda N

    Plos One   17 ( 1 )   e0261870   2022.1

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    DOI: 10.1371/journal.pone.0261870.

  • 倫理・プロフェッショナリズム教育の現状

    平田 創一郎, 樫 則章, 二宮 一智, 長谷 由紀子, 和田 尚久

    The Journal of Japanese Dental Education Association   38 ( 3 )   134 - 138   2022   ISSN:09145133 eISSN:24331651

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  • Factors that influence the judgment of oral management necessity in preoperative oral screening. Reviewed International journal

    Kai N, Tsukamoto Y, Urabe K, Tani A, Inai Y, Okadome A, Kashiwazaki H, Mizutani S, Wada N

    Int J Environ Res   18 ( 22 )   12236   2021.1

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    DOI: 10.3390/ijerph182212236.

  • Functions of beta2-adrenergic receptor in human periodontal ligament cells. Reviewed International journal

    Hamano S, Tomokiyo A, Hasegawa D, Yuda A, Sugii H, Yoshida S, Mitarai H, Wada N, Maeda H

    J Cell Biochem   121 ( 12 )   4798 - 4808   2020.12

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    DOI: 10.1002/jcb.29706

  • Risk factors for postoperative pneumonia according to examination findings before surgery under general anesthesia. Reviewed International journal

    Inai Y, Nomura Y, Takarada T, Hanada N, Wada N

    Clin Oral Investig   24 ( 10 )   3577 - 3585   2020.10

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  • 新義歯製作により口腔機能の向上を目指した1症例-閉口印象およびフレンジテクニックを活用した新義歯製作- Reviewed

    掛村友起子、祐田明香、王丸寛美、和田尚久

    日本総合歯科学会雑誌   12 ( 1 )   70 - 77   2020.10

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  • 製作時期が異なる複数の既存義歯の問題点を考察し、新義歯製作に活かした1症例 Reviewed

    尾池麻未、伊吹貞一、和田尚久

    日本総合歯科学会雑誌   12 ( 1 )   57 - 64   2020.10

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  • MEST regulates the stemness of human periodontal ligament stem cells. Reviewed International journal

    Hasegawa D, Hasegawa K, Kaneko H, Yoshida S, Mitarai H, Arima M, Tomokiyo A, Hamano S, Sugii H, Wada N, Kiyoshima T, Maeda H

    Stem Cells Int.   2020.7

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    DOI: 10.1155/2020/9672673. 2020

  • Expression and function of dopamine in odontoblasts. Reviewed International journal

    Shoko Fujino, Sayuri Hamano, Atsushi Tomokiyo, Tomohiro Itoyama, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Ayako Washio, Aoi Nozu, Taiga Ono, Naohisa Wada, Chiaki Kitamura, Hidefumi Maeda

    Journal of cellular physiology   235 ( 5 )   4376 - 4387   2020.5

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    Dopamine (DA) is produced from tyrosine by tyrosine hydroxylase (TH). A recent study has reported that DA promotes the mineralization of murine preosteoblasts. However, the role of DA in odontoblasts has not been examined. Therefore, in this investigation, we researched the expression of TH and DA in odontoblasts and the effects of DA on the differentiation of preodontoblasts (KN-3 cells). Immunostaining showed that TH and DA were intensely expressed in odontoblasts and preodontoblasts of rat incisors and molars. KN-3 cells expressed D1-like and D2-like receptors for DA. Furthermore, DA promoted odontoblastic differentiation of KN-3 cells, whereas an antagonist of D1-like receptors and a PKA signaling blocker, inhibited such differentiation. However, antagonists of D2-like receptors promoted differentiation. These results suggested that DA in preodontoblasts and odontoblasts might promote odontoblastic differentiation through D1-like receptors, but not D2-like receptors, and PKA signaling in an autocrine or paracrine manner and plays roles in dentinogenesis.

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  • 肥大型心筋症患者の歯科治療を通じて歯科臨床研修における老年歯科医学の位置づけを考察した1例 Reviewed

    山添 淳一, 衛藤 希, 尾崎 礼奈, 倉田 理沙, 湯川 綾美, 祐田 明香, 稲井 裕子, 和田 尚久

    老年歯科医学   35 ( 3 )   218 - 225   2020.4

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  • Drinking Ice-Cold Water Reduces the Severity of Anticancer Drug-Induced Taste Dysfunction in Mice. Invited Reviewed International journal

    Osaki A, Sanematsu K, Yamazoe J, Hirose F, Watanabe Y, Kawabata Y, Oike A, Hirayama A, Yamada Y, Iwata S, Takai S, Wada N, Shigemura N

    Int J Mol Sci.   21 ( 23 )   8958   2020.4

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    DOI: 10.3390/ijms21238958

  • 口腔内3Dスキャナを用いて口腔内情報を採取し、個人識別に応用した1症例 Invited Reviewed

    衛藤 希, 山添 淳一, 奥村美紀,鮫島直美,辻彰子,和田 尚久,池田典昭

    法医学の実際と研究   63   17 - 22   2020.4

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  • Functions of beta2-adrenergic receptor in human periodontal ligament cells. Reviewed International journal

    Sayuri Hamano, Atsushi Tomokiyo, Daigaku Hasegawa, Asuka Yuda, Hideki Sugii, Shinichiro Yoshida, Hiromi Mitarai, Naohisa Wada, Hidefumi Maeda

    Journal of cellular biochemistry   2020.3

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    Adrenergic receptors (ARs) are receptors of noradrenalin and adrenalin, of which there are nine different subtypes. In particular, β2 adrenergic receptor (β2-AR) is known to be related to the restoration and maintenance of homeostasis in bone and cardiac tissues; however, the functional role of signaling through β2-AR in periodontal ligament (PDL) tissue has not been fully examined. In this report, we investigated that β2-AR expression in PDL tissues and their features in PDL cells. β2-AR expressed in rat PDL tissues and human PDL cells (HPDLCs) derived from two different patients (HPDLCs-2G and -3S). Rat PDL tissue with occlusal loading showed high β2-AR expression, while its expression was downregulated in that without loading. In HPDLCs, β2-AR expression was increased exposed to stretch loading. The gene expression of PDL-related molecules was investigated in PDL clone cells (2-23 cells) overexpressing β2-AR. Their gene expression and intracellular cyclic adenosine monophosphate (cAMP) levels were also investigated in HPDLCs treated with a specific β2-AR agonist, fenoterol (FEN). Overexpression of β2-AR significantly promoted the gene expression of PDL-related molecules in 2 to 23 cells. FEN led to an upregulation in the expression of PDL-related molecules and increased intracellular cAMP levels in HPDLCs. In both HPDLCs, inhibition of cAMP signaling by using protein kinase A inhibitor suppressed the FEN-induced gene expression of α-smooth muscle actin. Our findings suggest that the occlusal force is important for β2-AR expression in PDL tissue and β2-AR is involved in fibroblastic differentiation and collagen synthesis of PDL cells. The signaling through β2-AR might be important for restoration and homeostasis of PDL tissue.

    DOI: 10.1002/jcb.29706

  • Risk factors for postoperative pneumonia according to examination findings before surgery under general anesthesia. Reviewed International journal

    Yuko Inai, Yoshiaki Nomura, Tohru Takarada, Nobuhiro Hanada, Naohisa Wada

    Clinical oral investigations   2020.2

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    OBJECTIVE: This study was performed to determine the risk factors associated with postoperative complications after surgery under general anesthesia according to respiratory function test results and oral conditions. MATERIALS AND METHODS: Preoperative examination data were collected for 471 patients who underwent surgery under general anesthesia at the Medical Hospital of Kyusyu University. Respiratory function tests, oral examinations, and perioperative oral management were performed in all patients. The incidence of and risk factors for postoperative complications were investigated. Classification and regression tree analyses were performed to investigate the risk factors for postoperative complications. RESULTS: Among the 471 patients, 11 developed postoperative pneumonia, 10 developed postoperative respiratory symptoms, and 10 developed postoperative fever. The most important risk factor for pneumonia was edentulism. Age, the Brinkman index, and head and neck surgery were also revealed as important risk factors for pneumonia. The O'Leary plaque control record (initial visit) was an important risk factor for postoperative respiratory symptoms. With respect to postoperative fever, a Hugh-Jones classification of grade > 1 was the most important risk factor; edentulism and a Brinkman index of > 642.5 were also found to be risk factors. CONCLUSION: In addition to respiratory function tests, oral examinations may be important for the prediction of postoperative complications. Additionally, improved oral hygiene may be effective in preventing postoperative respiratory complications. CLINICAL RELEVANCE: Risk factors for postoperative complications should be comprehensively evaluated using both respiratory function tests and oral findings.

    DOI: 10.1007/s00784-020-03230-7

  • Expression and function of dopamine in odontoblasts Reviewed

    Shoko Fujino, Sayuri Hamano, Atsushi Tomokiyo, Tomohiro Itoyama, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Ayako Washio, Aoi Nozu, Taiga Ono, Naohisa Wada, Chiaki Kitamura, Hidefumi Maeda

    Journal of cellular physiology   235 ( 5 )   4376 - 4387   2020.1

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    Dopamine (DA) is produced from tyrosine by tyrosine hydroxylase (TH). A recent study has reported that DA promotes the mineralization of murine preosteoblasts. However, the role of DA in odontoblasts has not been examined. Therefore, in this investigation, we researched the expression of TH and DA in odontoblasts and the effects of DA on the differentiation of preodontoblasts (KN-3 cells). Immunostaining showed that TH and DA were intensely expressed in odontoblasts and preodontoblasts of rat incisors and molars. KN-3 cells expressed D1-like and D2-like receptors for DA. Furthermore, DA promoted odontoblastic differentiation of KN-3 cells, whereas an antagonist of D1-like receptors and a PKA signaling blocker, inhibited such differentiation. However, antagonists of D2-like receptors promoted differentiation. These results suggested that DA in preodontoblasts and odontoblasts might promote odontoblastic differentiation through D1-like receptors, but not D2-like receptors, and PKA signaling in an autocrine or paracrine manner and plays roles in dentinogenesis.

    DOI: 10.1002/jcp.29314

  • Oral Management Due to Denture Adjustment in a Frail Patient with Spinocerebellar Degeneration Contributed to Effective Rehabilitation Reviewed

    Osada K, Yugawa A, Yamazoe J, Wada N

    老年歯科医学   35 ( 1 )   61 - 69   2020.1

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  • Periodontal Ligament Stem Cells: Regenerative Potency in Periodontium. Reviewed International journal

    Atsushi Tomokiyo, Naohisa Wada, Hidefumi Maeda

    Stem cells and development   28 ( 15 )   974 - 985   2019.8

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    Periodontium is consisted of root cementum, bone lining the tooth socket, gingiva facing the tooth, and periodontal ligament (PDL). Its primary functions are support of the tooth and protection of tooth, nerve, and blood vessels from injury by mechanical loading. Severe periodontitis induces the destruction of periodontium and results in a significant cause of tooth loss among adults. Unfortunately, conventional therapies such as scaling and root planning are often only palliative. Therefore, the ultimate goal of the treatment for periodontitis is to restore disrupted periodontium to its original shape and function. Tissue engineering refers to the process of combining cells, scaffolds, and signaling molecules for the production of functional tissues to restore, maintain, and improve damaged organs. The discovery of periodontal ligament stem cells (PDLSCs) highlighted the possibility for development of tissue engineering technology-based therapeutics for disrupted periodontium. PDLSCs are a kind of somatic stem cells that show potential to differentiate into multiple cell types and undergo robust clonal self-renewal. Therefore, PDLSCs are considered a highly promising stem cell population for regenerative therapy in periodontium; however, their rarity prevents the progression of basic and clinical researches. In this review, we summarize recent research advancement and accumulated information regarding the self-renewal capacity, multipotency, and immunomodulatory effect of PDLSCs, as well as their contribution to repair and regeneration of periodontium and other tissues. We also discuss the possibility of PDLSCs for clinical application of regenerative medicine and provide an outline of the genetic approaches to overcome the issue about the rarity of PDLSCs.

    DOI: 10.1089/scd.2019.0031

  • R-spondin 2 promotes osteoblastic differentiation of immature human periodontal ligament cells through the Wnt/β-catenin signaling pathway Reviewed

    Mai Arima, Daigaku Hasegawa, Shinichiro Yoshida, Hiromi Mitarai, Atsushi Tomokiyo, Sayuri Hamano, Hideki Sugii, Naohisa Wada, Hidefumi Maeda

    Journal of Periodontal Research   54 ( 2 )   143 - 153   2019.4

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    Objective: In this study, we measured the expression of R-spondin 2 (RSPO2) in periodontal ligament (PDL) tissue and cells. Further, we examined the effects of RSPO2 on osteoblastic differentiation of immature human PDL cells (HPDLCs). Background: R-spondin (RSPO) family proteins are secreted glycoproteins that play important roles in embryonic development and tissue homeostasis through activation of the Wnt/β-catenin signaling pathway. RSPO2, a member of the RSPO family, has been reported to enhance osteogenesis in mice. However, little is known regarding the roles of RSPO2 in PDL tissues. Methods: Expression of RSPO2 in rat PDL tissue and primary HPDLCs was examined by immunohistochemical and immunofluorescence staining, as well as by semiquantitative RT-PCR. The effects of stretch loading on the expression of RSPO2 and Dickkopf-related protein 1 (DKK1) were assessed by quantitative RT-PCR. Expression of receptors for RSPOs, such as Leucine-rich repeat-containing G-protein-coupled receptors (LGRs) 4, 5, and 6 in immature human PDL cells (cell line 2-14, or 2-14 cells), was investigated by semiquantitative RT-PCR. Mineralized nodule formation in 2-14 cells treated with RSPO2 under osteoblastic inductive condition was examined by Alizarin Red S and von Kossa stainings. Nuclear translocation of β-catenin and expression of active β-catenin in 2-14 cells treated with RSPO2 were assessed by immunofluorescence staining and Western blotting analysis, respectively. In addition, the effect of Dickkopf-related protein 1 (DKK1), an inhibitor of Wnt/β-catenin signaling, was also examined. Results: Rat PDL tissue and HPDLCs expressed RSPO2, and HPDLCs also expressed RSPO2, while little was found in 2-14 cells. Expression of RSPO2 as well as DKK1 in HPDLCs was significantly upregulated by exposure to stretch loading. LGR4 was predominantly expressed in 2-14 cells, which expressed low levels of LGR5 and LGR6. RSPO2 enhanced the Alizarin Red S and von Kossa-positive reactions in 2-14 cells. In addition, DKK1 suppressed nuclear translocation of β-catenin, activation of β-catenin, and increases of Alizarin Red S and von Kossa-positive reactions in 2-14 cells, all of which were induced by RSPO2 treatment. Conclusion: RSPO2, which is expressed in PDL tissue and cells, might play an important role in regulating the osteoblastic differentiation of immature human PDL cells through the Wnt/β-catenin signaling pathway.

    DOI: 10.1111/jre.12611

  • R-spondin 2 promotes osteoblastic differentiation of immature human periodontal ligament cells through the Wnt/β-catenin signaling pathway. Reviewed International journal

    Mai Arima, Daigaku Hasegawa, Shinichiro Yoshida, Hiromi Mitarai, Atsushi Tomokiyo, Sayuri Hamano, Hideki Sugii, Naohisa Wada, Hidefumi Maeda

    Journal of periodontal research   54 ( 2 )   143 - 153   2019.4

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    OBJECTIVE: In this study, we measured the expression of R-spondin 2 (RSPO2) in periodontal ligament (PDL) tissue and cells. Further, we examined the effects of RSPO2 on osteoblastic differentiation of immature human PDL cells (HPDLCs). BACKGROUND: R-spondin (RSPO) family proteins are secreted glycoproteins that play important roles in embryonic development and tissue homeostasis through activation of the Wnt/β-catenin signaling pathway. RSPO2, a member of the RSPO family, has been reported to enhance osteogenesis in mice. However, little is known regarding the roles of RSPO2 in PDL tissues. METHODS: Expression of RSPO2 in rat PDL tissue and primary HPDLCs was examined by immunohistochemical and immunofluorescence staining, as well as by semiquantitative RT-PCR. The effects of stretch loading on the expression of RSPO2 and Dickkopf-related protein 1 (DKK1) were assessed by quantitative RT-PCR. Expression of receptors for RSPOs, such as Leucine-rich repeat-containing G-protein-coupled receptors (LGRs) 4, 5, and 6 in immature human PDL cells (cell line 2-14, or 2-14 cells), was investigated by semiquantitative RT-PCR. Mineralized nodule formation in 2-14 cells treated with RSPO2 under osteoblastic inductive condition was examined by Alizarin Red S and von Kossa stainings. Nuclear translocation of β-catenin and expression of active β-catenin in 2-14 cells treated with RSPO2 were assessed by immunofluorescence staining and Western blotting analysis, respectively. In addition, the effect of Dickkopf-related protein 1 (DKK1), an inhibitor of Wnt/β-catenin signaling, was also examined. RESULTS: Rat PDL tissue and HPDLCs expressed RSPO2, and HPDLCs also expressed RSPO2, while little was found in 2-14 cells. Expression of RSPO2 as well as DKK1 in HPDLCs was significantly upregulated by exposure to stretch loading. LGR4 was predominantly expressed in 2-14 cells, which expressed low levels of LGR5 and LGR6. RSPO2 enhanced the Alizarin Red S and von Kossa-positive reactions in 2-14 cells. In addition, DKK1 suppressed nuclear translocation of β-catenin, activation of β-catenin, and increases of Alizarin Red S and von Kossa-positive reactions in 2-14 cells, all of which were induced by RSPO2 treatment. CONCLUSION: RSPO2, which is expressed in PDL tissue and cells, might play an important role in regulating the osteoblastic differentiation of immature human PDL cells through the Wnt/β-catenin signaling pathway.

    DOI: 10.1111/jre.12611

  • Wnt/β-catenin signaling, which is activated in odontomas, reduces Sema3A expression to regulate odontogenic epithelial cell proliferation and tooth germ development. Reviewed

    Fujii S, Nagata K, Matsumoto S, Kohashi KI, Kikuchi A, Oda Y, Kiyoshima T, Wada N

    Scientific reports   9 ( 1 )   4257   2019.3

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    Wnt/β-catenin signaling, which is activated in odontomas, reduces Sema3A expression to regulate odontogenic epithelial cell proliferation and tooth germ development.

    DOI: 10.1038/s41598-019-39686-1

  • 認知症の舌癌患者に対し、周術期口腔機能管理を通して経口摂取支援を行った一症例 Reviewed

    湯川綾美、山添淳一、和智(千北)さとみ、山田朋弘、和田尚久

    老年歯科医学   34 ( 2 )   136 - 142   2019.2

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  • 近年の九州地区における大学病院口腔総合診療医の災害支援活動に関する分析 Reviewed

    山添淳一、清水貴義、内藤久貴、湯川綾美、衛藤希、和田尚久

    日本総合歯科学会雑誌   11 ( 1 )   20 - 30   2019.1

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  • 総合的な情報収集により良好な治療結果をもたらしたインレー再製作の1症例 Reviewed

    阿瀉濱陽子、伊吹貞一、和田尚久

    日本総合歯科学会雑誌   11 ( 1 )   91 - 98   2019.1

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  • 骨組織上に播種した歯髄幹細胞は歯根膜関連遺伝子を発現する 接触する基質の硬さが細胞分化に及ぼす影響

    吉田 晋一郎, 和田 尚久, 長谷川 大学, 御手洗 裕美, 有馬 麻衣, 友清 淳, 濱野 さゆり, 杉井 英樹, 前田 英史

    日本歯科保存学雑誌   61 ( 6 )   343 - 353   2018.12

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    目的:重篤な歯周炎や外傷などのケースにおいては、歯槽骨を含め歯の支持組織である歯根膜組織の破壊が進む。ゆえに、歯根膜組織再生を誘導することができる細胞移植を用いた新規歯周組織再生療法の開発が望まれている。歯髄組織には歯髄幹細胞が豊富に含まれており、再生医療の現場では重要な細胞源として注目されている。近年、間葉系幹細胞は接する基質の硬さにより分化の運命が方向づけられるとの報告がされている。そこで本研究では、歯根膜スペースへ移植した歯髄細胞が、接する基質の硬さを触知して歯根膜様細胞分化を遂げる能力を有する可能性を考え、歯周組織を再生させる細胞源としての有用性について検討することとした。材料と方法:ヒト歯髄細胞およびヒト歯根膜細胞は、全身疾患を有さない抜歯目的の患者より抜去した歯から単離し、またヒト歯髄幹細胞およびヒト歯根膜幹細胞は、それぞれヒト歯髄細胞およびヒト歯根膜細胞からsingle colony selectionにより単離して用いた。各種遺伝子発現を、定量的RT-PCR法を用いて検討した。骨芽細胞・脂肪細胞および軟骨細胞誘導実験は、alizarin red S染色、oil red O染色およびalcian blue染色によって評価した。細胞表面抗原の発現を、フローサイトメーターを用いて測定した。健全なブタ下顎骨から縦10mm×横10mm×高さ3mmに整形したブタ顎骨スライスを採取し、実験に用いた。ラットGFP発現歯髄細胞の歯根膜組織へのホーミングについて、ラット第一臼歯の意図的再植時に、抜歯窩へ細胞移植を行い、免疫組織化学的染色法を用いて解析した。成績:ヒト歯髄細胞とヒト歯根膜細胞における歯根膜関連因子の遺伝子発現を比較した結果、ヒト歯根膜細胞において有意な高発現を認めた。ヒト歯髄幹細胞およびヒト歯根膜幹細胞は、多分化能を示し幹細胞マーカーを高発現していた。また、ヒト歯髄細胞およびヒト歯根膜細胞と同様に、ヒト歯髄幹細胞と比較してヒト歯根膜幹細胞は歯根膜関連遺伝子の高発現を認めた。ブタ顎骨スライス上でヒト歯髄幹細胞を培養した結果、歯根膜関連遺伝子発現は、培養ディッシュ上で培養した歯髄幹細胞と比較して有意に上昇しており、その発現量はヒト歯根膜幹細胞と同等であった。加えて、抜歯窩に移植したラットGFP発現歯髄細胞は、再植歯周囲の歯根膜組織内に多く生着していることが認められた。結論:ヒト歯髄幹細胞を骨基質上で培養すると歯根膜関連遺伝子発現が上昇し、歯根膜組織を再生させる細胞源として有用である可能性が示唆された。(著者抄録)

  • Gender-dependent associations between occupational status and untreated caries in Japanese adults Reviewed

    Yuriko Harada, Kenji Takeuchi, Michiko Furuta, Akihiko Tanaka, Shunichi Tanaka, Naohisa Wada, Yoshihisa Yamashita

    Industrial health   56 ( 6 )   539 - 544   2018.11

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    The aim of this study was to examine whether the presence of untreated caries is different across occupational status among Japanese adults. This was a cross-sectional survey of 1,342 individuals (990 males and 352 females) aged 40-64 yr who underwent medical and dental checkups at a healthcare center in 2011. Oral examination was performed by a dentist and the presence of untreated caries was defined as having at least one untreated decayed tooth. Data regarding current occupational status were obtained using a self-administered questionnaire; the participants were classified into five groups: professionals and managers, clerical and related workers, service and salespersons, agricultural, forestry, and fishery workers, and homemakers and unemployed. Gender-specific odds ratios (ORs) and 95% confidence intervals (CIs) of occupational status for the presence of untreated caries were estimated using logistic regression. After adjusting for potential confounders, female professionals and managers (OR=3.51, 95% CI=1.04-11.87) and service and salespersons (OR=5.29, 95% CI=1.39-20.11) had greater risks of the presence of untreated caries than female homemakers and unemployed. However, this tendency was not observed among males. In conclusion, there was a significant difference in risk of the presence of untreated caries by occupational status among females.

    DOI: 10.2486/indhealth.2018-0062

  • Gender-dependent associations between occupational status and untreated caries in Japanese adults. Reviewed

    Yuriko Harada, Kenji Takeuchi, Michiko Furuta, Akihiko Tanaka, Shunichi Tanaka, Naohisa Wada, Yoshihisa Yamashita

    Industrial health   56 ( 6 )   539 - 544   2018.11

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    The aim of this study was to examine whether the presence of untreated caries is different across occupational status among Japanese adults. This was a cross-sectional survey of 1,342 individuals (990 males and 352 females) aged 40-64 yr who underwent medical and dental checkups at a healthcare center in 2011. Oral examination was performed by a dentist and the presence of untreated caries was defined as having at least one untreated decayed tooth. Data regarding current occupational status were obtained using a self-administered questionnaire; the participants were classified into five groups: professionals and managers, clerical and related workers, service and salespersons, agricultural, forestry, and fishery workers, and homemakers and unemployed. Gender-specific odds ratios (ORs) and 95% confidence intervals (CIs) of occupational status for the presence of untreated caries were estimated using logistic regression. After adjusting for potential confounders, female professionals and managers (OR=3.51, 95% CI=1.04-11.87) and service and salespersons (OR=5.29, 95% CI=1.39-20.11) had greater risks of the presence of untreated caries than female homemakers and unemployed. However, this tendency was not observed among males. In conclusion, there was a significant difference in risk of the presence of untreated caries by occupational status among females.

    DOI: 10.2486/indhealth.2018-0062

  • Wnt5a suppresses osteoblastic differentiation of human periodontal ligament stem cell-like cells via Ror2/JNK signaling. Reviewed International journal

    Daigaku Hasegawa, Naohisa Wada, Shinichiro Yoshida, Hiromi Mitarai, Mai Arima, Atsushi Tomokiyo, Sayuri Hamano, Hideki Sugii, Hidefumi Maeda

    Journal of cellular physiology   233 ( 2 )   1752 - 1762   2018.2

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    Wnt5a, a non-canonical Wnt protein, is known to play important roles in several cell functions. However, little is known about the effects of Wnt5a on osteoblastic differentiation of periodontal ligament (PDL) cells. Here, we examined the effects of Wnt5a on osteoblastic differentiation and associated intracellular signaling in human PDL stem/progenitor cells (HPDLSCs). We found that Wnt5a suppressed expression of bone-related genes (ALP, BSP, and Osterix) and alizarin red-positive mineralized nodule formation in HPDLSCs under osteogenic conditions. Immunohistochemical analysis revealed that a Wnt5a-related receptor, receptor tyrosine kinase-like orphan receptor 2 (Ror2), was expressed in rat PDL tissue. Interestingly, knockdown of Ror2 by siRNA inhibited the Wnt5a-induced downregulation of bone-related gene expression in HPDLSCs. Moreover, Western blotting analysis showed that phosphorylation of the intracellular signaling molecule, c-Jun N-terminal kinase (JNK) was upregulated in HPDLSCs cultured in osteoblast induction medium with Wnt5a, but knockdown of Ror2 by siRNA downregulated the phosphorylation of JNK. We also examined the effects of JNK inhibition on Wnt5a-induced suppression of osteoblastic differentiation of HPDLSCs. The JNK inhibitor, SP600125 inhibited the Wnt5a-induced downregulation of bone-related gene expression in HPDLSCs. Additionally, SP600125 inhibited the Wnt5a-induced suppression of the alizarin red-positive reaction in HPDLSCs. These results suggest that Wnt5a suppressed osteoblastic differentiation of HPDLSCs through Ror2/JNK signaling. Non-canonical Wnt signaling, including Wnt5a/Ror2/JNK signaling, may function as a negative regulator of mineralization, preventing the development of non-physiological mineralization in PDL tissue.

    DOI: 10.1002/jcp.26086

  • Senescence and odontoblastic differentiation of dental pulp cells Reviewed

    Aoi Nozu, Sayuri Hamano, Atsushi Tomokiyo, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Hiromi Mitarai, Shuntaro Taniguchi, Naohisa Wada, Hidefumi Maeda

    Journal of cellular physiology   234 ( 1 )   849 - 859   2018.1

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    Cellular senescence has been suggested to be involved in physiological changes of cytokine production. Previous studies showed that the concentration of tumor necrosis factor-α (TNF-α) is higher in the blood of aged people compared with that of young people. So far, the precise effects of TNF-α on the odontoblastic differentiation of pulp cells have been controversial. Therefore, we aimed to clarify how this cytokine affected pulp cells during aging. Human dental pulp cells (HDPCs) were cultured until reaching the plateau of their growth, and the cells were isolated at actively (young HDPCs; yHDPCs) or inactively (senescent HDPCs; sHDPCs) proliferating stages. sHDPCs expressed senescence-related molecules while yHDPCs did not. When these HDPCs were cultured in an odontoblast-inductive medium, both young and senescent cells showed mineralization, but mineralization in sHDPCs was lower compared with yHDPCs. However, the administration of TNF-α to this culture medium altered these responses: yHDPCs showed downregulated mineralization, while sHDPCs exhibited significantly increased mineralization. Furthermore, the expression of tumor necrosis factor receptor 1 (TNFR1), a receptor of TNF-α, was significantly upregulated in sHDPCs compared with yHDPCs. Downregulation of TNFR1 expression led to decreased mineralization of TNF-α-treated sHDPCs, whereas restored the reduction in TNF-α-treated yHDPCs. These results suggested that sHDPCs preserved the odontoblastic differentiation capacity and TNF-α promoted odontoblastic differentiation of HDPCs with the progress of their population doublings through increased expression of TNFR1. Thus, TNF-α might exert a different effect on the odontoblastic differentiation of HDPCs depending on their proliferating activity. In addition, the calcification of pulp chamber with age may be related with increased reactivity of pulp cells to TNF-α.

    DOI: 10.1002/jcp.26905

  • Senescence and odontoblastic differentiation of dental pulp cells. Reviewed International journal

    Aoi Nozu, Sayuri Hamano, Atsushi Tomokiyo, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Hiromi Mitarai, Shuntaro Taniguchi, Naohisa Wada, Hidefumi Maeda

    Journal of cellular physiology   234 ( 1 )   849 - 859   2018.1

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    Cellular senescence has been suggested to be involved in physiological changes of cytokine production. Previous studies showed that the concentration of tumor necrosis factor-α (TNF-α) is higher in the blood of aged people compared with that of young people. So far, the precise effects of TNF-α on the odontoblastic differentiation of pulp cells have been controversial. Therefore, we aimed to clarify how this cytokine affected pulp cells during aging. Human dental pulp cells (HDPCs) were cultured until reaching the plateau of their growth, and the cells were isolated at actively (young HDPCs; yHDPCs) or inactively (senescent HDPCs; sHDPCs) proliferating stages. sHDPCs expressed senescence-related molecules while yHDPCs did not. When these HDPCs were cultured in an odontoblast-inductive medium, both young and senescent cells showed mineralization, but mineralization in sHDPCs was lower compared with yHDPCs. However, the administration of TNF-α to this culture medium altered these responses: yHDPCs showed downregulated mineralization, while sHDPCs exhibited significantly increased mineralization. Furthermore, the expression of tumor necrosis factor receptor 1 (TNFR1), a receptor of TNF-α, was significantly upregulated in sHDPCs compared with yHDPCs. Downregulation of TNFR1 expression led to decreased mineralization of TNF-α-treated sHDPCs, whereas restored the reduction in TNF-α-treated yHDPCs. These results suggested that sHDPCs preserved the odontoblastic differentiation capacity and TNF-α promoted odontoblastic differentiation of HDPCs with the progress of their population doublings through increased expression of TNFR1. Thus, TNF-α might exert a different effect on the odontoblastic differentiation of HDPCs depending on their proliferating activity. In addition, the calcification of pulp chamber with age may be related with increased reactivity of pulp cells to TNF-α.

    DOI: 10.1002/jcp.26905

  • Extracellular Matrix from Periodontal Ligament Cells Could Induce the Differentiation of Induced Pluripotent Stem Cells to Periodontal Ligament Stem Cell-Like Cells. Reviewed International journal

    Sayuri Hamano, Atsushi Tomokiyo, Daigaku Hasegawa, Shinichiro Yoshida, Hideki Sugii, Hiromi Mitarai, Shoko Fujino, Naohisa Wada, Hidefumi Maeda

    Stem cells and development   27 ( 2 )   100 - 111   2018.1

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    The periodontal ligament (PDL) plays an important role in anchoring teeth in the bone socket. Damage to the PDL, such as after severe inflammation, can be treated with a therapeutic strategy that uses stem cells derived from PDL tissue (PDLSCs), a strategy that has received intense scrutiny over the past decade. However, there is an insufficient number of PDLSCs within the PDL for treating such damage. Therefore, we sought to induce the differentiation of induced pluripotent stem (iPS) cells into PDLSCs as an initial step toward PDL therapy. To this end, we first induced iPS cells into neural crest (NC)-like cells. We then captured the p75 neurotrophic receptor-positive cells (iPS-NC cells) and cultured them on an extracellular matrix (ECM) produced by human PDL cells (iPS-NC-PDL cells). These iPS-NC-PDL cells showed reduced expression of embryonic stem cell and NC cell markers as compared with iPS and iPS-NC cells, and enrichment of mesenchymal stem cell markers. The cells also had a higher proliferative capacity, multipotency, and elevated expression of PDL-related markers than iPS-NC cells cultured on fibronectin and laminin (iPS-NC-FL cells) or ECM produced by human skin fibroblast cells (iPS-NC-SF cells). Overall, we present a culture method to produce high number of PDLSC-like cells from iPS cells as a first step toward a strategy for PDL regeneration.

    DOI: 10.1089/scd.2017.0077

  • 近い将来の咬合崩壊が予測された高齢患者の1例 Reviewed

    佐野大成、伊吹禎一、和田尚久

    日本総合歯科学会雑誌   10 ( 1 )   55 - 60   2018.1

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  • Extracellular Matrix from Periodontal Ligament Cells Could Induce the Differentiation of Induced Pluripotent Stem Cells to Periodontal Ligament Stem Cell-Like Cells Reviewed

    Sayuri Hamano, Atsushi Tomokiyo, Daigaku Hasegawa, Shinichiro Yoshida, Hideki Sugii, Hiromi Mitarai, Shoko Fujino, Naohisa Wada, Hidefumi Maeda

    Stem Cells and Development   27 ( 2 )   100 - 111   2018.1

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    The periodontal ligament (PDL) plays an important role in anchoring teeth in the bone socket. Damage to the PDL, such as after severe inflammation, can be treated with a therapeutic strategy that uses stem cells derived from PDL tissue (PDLSCs), a strategy that has received intense scrutiny over the past decade. However, there is an insufficient number of PDLSCs within the PDL for treating such damage. Therefore, we sought to induce the differentiation of induced pluripotent stem (iPS) cells into PDLSCs as an initial step toward PDL therapy. To this end, we first induced iPS cells into neural crest (NC)-like cells. We then captured the p75 neurotrophic receptor-positive cells (iPS-NC cells) and cultured them on an extracellular matrix (ECM) produced by human PDL cells (iPS-NC-PDL cells). These iPS-NC-PDL cells showed reduced expression of embryonic stem cell and NC cell markers as compared with iPS and iPS-NC cells, and enrichment of mesenchymal stem cell markers. The cells also had a higher proliferative capacity, multipotency, and elevated expression of PDL-related markers than iPS-NC cells cultured on fibronectin and laminin (iPS-NC-FL cells) or ECM produced by human skin fibroblast cells (iPS-NC-SF cells). Overall, we present a culture method to produce high number of PDLSC-like cells from iPS cells as a first step toward a strategy for PDL regeneration.

    DOI: 10.1089/scd.2017.0077

  • Calcium-sensing receptor-ERK signaling promotes odontoblastic differentiation of human dental pulp cells. Reviewed International journal

    Hiroyuki Mizumachi, Shinichiro Yoshida, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, Asuka Yuda, Hideki Sugii, Suguru Serita, Hiromi Mitarai, Katsuaki Koori, Naohisa Wada, Hidefumi Maeda

    Bone   101   191 - 201   2017.8

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    Activation of the G protein-coupled calcium-sensing receptor (CaSR) has crucial roles in skeletal development and bone turnover. Our recent study has identified a role for activated CaSR in the osteogenic differentiation of human periodontal ligament stem cells. Furthermore, odontoblasts residing inside the tooth pulp chamber play a central role in dentin formation. However, it remains unclear how CaSR activation affects the odontoblastic differentiation of human dental pulp cells (HDPCs). We have investigated the odontoblastic differentiation of HDPCs exposed to elevated levels of extracellular calcium (Ca) and strontium (Sr), and the contribution of CaSR and the L-type voltage-dependent calcium channel (L-VDCC) to this process. Immunochemical staining of rat dental pulp tissue demonstrated that CaSR was expressed at high levels in the odontoblastic layer, moderate levels in the sublayer, and low levels in the central pulp tissue. Although normal HDPCs expressed low levels of CaSR, stimulation with Ca or Sr promoted both CaSR expression and odontoblastic differentiation of HDPCs along with increased expression of odontoblastic makers. These effects were inhibited by treatment with a CaSR antagonist, whereas treatment with an L-VDCC inhibitor had no effect. Additionally, knockdown of CaSR with siRNA suppressed odontoblastic differentiation of Ca- and Sr-treated HDPCs. ERK1/2 phosphorylation was observed in Ca- and Sr-treated HDPCs, whereas CaSR antagonist treatment or CaSR knockdown blocked ERK1/2 phosphorylation. Furthermore, inhibition of ERK1/2 suppressed mineralization of Ca- and Sr-treated HDPCs. These results suggest that elevated concentrations of extracellular Ca and Sr induce odontoblastic differentiation of HDPCs through CaSR activation and the ERK1/2 phosphorylation.

    DOI: 10.1016/j.bone.2017.05.012

  • 歯内治療ならびに修復処置関連溶液によって生じるWhite Mineral Trioxide Aggregateの色調変化に関する比較分析

    友清 淳, 濱野 さゆり, 長谷川 大学, 杉井 英樹, 吉田 晋一郎, 御手洗 裕美, 有馬 麻衣, 野津 葵, 和田 尚久, 前田 英史

    日本歯科保存学雑誌   60 ( 4 )   200 - 210   2017.8

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    目的:直接覆髄や穿孔部封鎖に用いたWhite Mineral Trioxide Aggregate(WMTA)が,往々にして色調変化をきたすことが知られている一方で,その色調と浸透深さに関する研究報告は少ない.そこで本研究は,歯内治療ならびに修復処置に関連する7種類の溶液を用い,それらによって生じるWMTAの色調変化パターンや浸透深さについて検証することを目的とした.材料と方法:WMTAを蒸留水(DW)と通法に従い混和したものを型枠に填入し,硬化させることによりWMTA diskを作製した.これらのdiskを次亜塩素酸ナトリウム溶液(以下,NaClO),エチレンジアミン四酢酸溶液(以下,EDTA),過酸化水素溶液(以下,H2O2),ボンディング材(以下,BOND),血液(以下,BLOOD),I型コラーゲン溶液(以下,COL1),およびヨウ素溶液(以下,JG)に浸漬し,またコントロールとしてDWに浸漬した.1,7,14日経過後に測色装置および実体顕微鏡による画像撮影を行った.さらに実体顕微鏡画像に対しては,画像補正用カラーチャートを基に画像補正を行った.次に浸漬後のdiskを割断し,実体顕微鏡下にて割断面の画像撮影を行った.また,酸化ビスマスをDW,NaClO,BLOOD,JGに浸漬し,1,7,14日経過後,WMTA diskと同様の画像撮影および画像補正を行った.これらの画像を用いて,1試料当たり5点の色調測定を行い,平均値および標準偏差を算出した.結果:EDTA,H2O2,BOND,COL1に浸漬したdiskは,DWに浸漬したものと同様の色調を示したが,NaClO,BLOOD,JGに浸漬したdiskには色調変化が生じた.Diskの割断面を観察した結果,NaClOおよびJGに浸漬したdiskでは内部まで色調変化が生じたのに対し,BLOODに浸漬したものでは,内部に色調変化は生じなかった.DW,EDTA,H2O2,BOND,COL1に浸漬したdiskにおいても,内部に色調変化は認められなかった.一方NaClO,BLOOD,JGに浸漬した酸化ビスマスにおいても色調変化が生じたが,WMTA diskの色調変化パターンとは異なっていた.結論:次亜塩素酸ナトリウム溶液,血液,ヨウ素溶液は,硬化後のWMTAの色調変化を引き起こすが,それらの色調や浸透深さは異なることが明らかとなった.(著者抄録)

  • Transforming growth factor-β-induced gene product-h3 inhibits odontoblastic differentiation of dental pulp cells. Reviewed International journal

    Suguru Serita, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, Hideki Sugii, Shinichiro Yoshida, Hiroyuki Mizumachi, Hiromi Mitarai, Satoshi Monnouchi, Naohisa Wada, Hidefumi Maeda

    Archives of oral biology   78   135 - 143   2017.6

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    OBJECTIVE: The aim of this study was to investigate transforming growth factor-β-induced gene product-h3 (βig-h3) expression in dental pulp tissue and its effects on odontoblastic differentiation of dental pulp cells (DPCs). DESIGN: A rat direct pulp capping model was prepared using perforated rat upper first molars capped with mineral trioxide aggregate cement. Human DPCs (HDPCs) were isolated from extracted teeth. βig-h3 expression in rat dental pulp tissue and HDPCs was assessed by immunostaining. Mineralization of HDPCs was assessed by Alizarin red-S staining. Odontoblast-related gene expression in HDPCs was analyzed by quantitative RT-PCR. RESULTS: Expression of βig-h3 was detected in rat dental pulp tissue, and attenuated by direct pulp capping, while expression of interleukin-1β and tumor necrosis factor-α was increased in exposed pulp tissue. βig-h3 expression was also detected in HDPCs, with reduced expression during odontoblastic differentiation. The above cytokines reduced βig-h3 expression in HDPCs, and promoted their mineralization. Recombinant βig-h3 inhibited the expression of odontoblast-related genes and mineralization of HDPCs, while knockdown of βig-h3 gene expression promoted the expression of odontoblast-related genes in HDPCs. CONCLUSIONS: The present findings suggest that βig-h3 in DPCs may be involved in reparative dentin formation and that its expression is likely to negatively regulate this process.

    DOI: 10.1016/j.archoralbio.2017.02.018

  • 歯内治療ならびに修復処置関連溶液によって生じるWhite Mineral Trioxide Aggregateの色調変化に関する比較分析 Reviewed

    友清淳, 濱野さゆり, 長谷川大学, 杉井英樹, 吉田晋一郎, 御手洗裕美, 有馬麻衣, 野津葵, 和田尚久, 前田英史

    日本歯科保存学雑誌   60 ( 4 )   200 - 210   2017.4

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  • GDNF From Human Periodontal Ligament Cells Treated With Pro-Inflammatory Cytokines Promotes Neurocytic Differentiation of PC12 Cells. Reviewed International journal

    Shinichiro Yoshida, Naohide Yamamoto, Naohisa Wada, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, Hiromi Mitarai, Satoshi Monnouchi, Asuka Yuda, Hidefumi Maeda

    Journal of cellular biochemistry   118 ( 4 )   699 - 708   2017.4

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    Glial cell line-derived neurotrophic factor (GDNF) is known to mediate multiple biological activities such as promotion of cell motility and proliferation, and morphogenesis. However, little is known about its effects on periodontal ligament (PDL) cells. Recently, we reported that GDNF expression is increased in wounded rat PDL tissue and human PDL cells (HPDLCs) treated with pro-inflammatory cytokines. Here, we investigated the associated expression of GDNF and the pro-inflammatory cytokine interleukin-1 beta (IL-1β) in wounded PDL tissue, and whether HPDLCs secrete GDNF which affects neurocytic differentiation. Rat PDL cells near the wounded area showed intense immunoreactions against an anti-GDNF antibody, where immunoreactivity was also increased against an anti-IL-1β antibody. Compared with untreated cells, HPDLCs treated with IL-1β or tumor necrosis factor-alpha showed an increase in the secretion of GDNF protein. Conditioned medium of IL-1β-treated HPDLCs (IL-1β-CM) increased neurite outgrowth of PC12 rat adrenal pheochromocytoma cells. The expression levels of two neural regeneration-associated genes, growth-associated protein-43 (Gap-43), and small proline-rich repeat protein 1A (Sprr1A), were also upregulated in IL-1β-CM-treated PC12 cells. These stimulatory effects of IL-1β-CM were significantly inhibited by a neutralizing antibody against GDNF. In addition, U0126, a MEK inhibitor, inhibited GDNF-induced neurite outgrowth of PC12 cells. These findings suggest that an increase of GDNF in wounded PDL tissue might play an important role in neural regeneration probably via the MEK/ERK signaling pathway. J. Cell. Biochem. 118: 699-708, 2017. © 2016 Wiley Periodicals, Inc.

    DOI: 10.1002/jcb.25662

  • Transforming growth factor-β-induced gene product-h3 inhibits odontoblastic differentiation of dental pulp cells. Reviewed International journal

    Suguru Serita, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, Hideki Sugii, Shinichiro Yoshida, H. Mizumachi, Hiromi Mitarai, S. Monnouchi, Naohisa Wada, Hidefumi Maeda

    Arch Oral Biol   78   135 - 143   2017.2

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    DOI: 10.1016/j.archoralbio.2017.02.018.

  • Calcium-sensing receptor-ERK signaling promotes odontoblastic differentiation of human dental pulp cells. Reviewed International journal

    H. Mizumachi, Shinichiro Yoshida, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, Asuka Yuda, Hideki Sugii, Suguru Serita, Hiromi Mitarai, K. Koori, Naohisa Wada, Hidefumi Maeda

    Bone   101   191 - 201   2017.2

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    DOI: 10.1016/j.bone.2017.05.012.

  • 全国歯科大学・歯学部における「2013年度版 よき歯科医師になるための20の質問 倫理的検討事例集」の利用状況 Reviewed

    山本龍生, 木尾哲郎, 尾崎哲則, 樫則章, 角忠輝, 平田創一郎, 和田 尚久, 平田幸夫

    日本歯科医学教育学科雑誌   2016.8

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  • 歯根膜および皮膚由来ヒト人工多能性幹細胞(iPSC)を用いた神経堤細胞様細胞の樹立とキャラクタリゼーション

    友清 淳, 和田 尚久, 濱野 さゆり, 長谷川 大学, 杉井 英樹, 吉田 晋一郎, 前田 英史

    日本歯科医師会雑誌   69 ( 5 )   480 - 480   2016.8

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  • Facioscapulohumeral muscular dystrophy (FSHD) region gene 1 (FRG1) expression and possible function in mouse tooth germ development. Reviewed International journal

    Kana Hasegawa, Hiroko Wada, Kengo Nagata, Hiroaki Fujiwara, Naohisa Wada, Hirotaka Someya, Yurie Mikami, Hidetaka Sakai, Tamotsu Kiyoshima

    Journal of molecular histology   47 ( 4 )   375 - 87   2016.8

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    Abnormal expression of Facioscapulohumeral muscular dystrophy (FSHD) region gene 1 (FRG1) is involved in the pathogenesis of FSHD. FRG1 is also important for the normal muscular and vascular development. Our previous study showed that FRG1 is one of the highly expressed genes in the mandible on embryonic day 10.5 (E10.5) than on E12.0. In this study, we investigated the temporospatial expression pattern of FRG1 mRNA and protein during the development of the mouse lower first molar, and also evaluated the subcellular localization of the FRG1 protein in mouse dental epithelial (mDE6) cells. The FRG1 expression was identified in the dental epithelial and mesenchymal cells at the initiation and bud stages. It was detected in the inner enamel epithelium at the cap and early bell stages. At the late bell and root formation stages, these signals were detected in ameloblasts and odontoblasts during the formation of enamel and dentin matrices, respectively. The FRG1 protein was localized in the cytoplasm in the mouse tooth germ in vivo, while FRG1 was detected predominantly in the nucleus and faintly in the cytoplasm in mDE6 cells in vitro. In mDE6 cells treated with bone morphogenetic protein 4 (BMP4), the protein expression of FRG1 increased in cytoplasm, suggesting that FRG1 may translocate to the cytoplasm. These findings suggest that FRG1 is involved in the morphogenesis of the tooth germ, as well as in the formation of enamel and dentin matrices and that FRG1 may play a role in the odontogenesis in the mouse following BMP4 stimulation.

    DOI: 10.1007/s10735-016-9680-5

  • GDNF from Human Periodontal Ligament Cells Treated with Proinflammatory Cytokines Promotes Neurocytic Differentiation of PC12 Cells. Reviewed International journal

    Shinichiro Yoshida, N. Yamamoto, Naohisa Wada, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, H. Mitarai, S. Monnouchi, A. Yuda, Hidefumi Maeda

    J Cell Biochem   118 ( 4 )   699 - 708   2016.7

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    DOI: 10.1002/jcb.25662

  • Facioscapulohumeral muscular dystrophy (FSHD) region gene 1 (FRG1) expression and possible function in mouse tooth germ development. Reviewed International journal

    Kana Hasegawa, Hiroko Wada, Kengo Nagata, Hiroaki Fujiwara, Naohisa Wada, Someya H, Mikami Y, Sakai Hidetaka, Tamotsu Kiyoshima

    J Mol Histol   47 ( 4 )   375 - 387   2016.5

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    DOI: 10.1007/s10735-016-9680-5

  • Benzo[a]pyrene/aryl hydrocarbon receptor signaling inhibits osteoblastic differentiation and collagen synthesis of human periodontal ligament cells. Reviewed International journal

    Monnouchi Satoshi, Hidefumi Maeda, Asuka Yuda, Suguru Serita, Naohisa Wada, Atsushi Tomokiyo, Akifumi Akamine

    J Periodontal Res   2016.1

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  • Wnt5a Induces Collagen Production by Human Periodontal Ligament Cells Through TGFβ1-Mediated Upregulation of Periostin Expression. Reviewed International journal

    Hasegawa D, Wada N, Maeda H, Yoshida S, Mitarai H, Tomokiyo A, Monnouchi S, Hamano S, Yuda A, Akamine A

    Journal of cellular physiology   230 ( 11 )   2647 - 60   2015.11

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    Wnt5a Induces Collagen Production by Human Periodontal Ligament Cells Through TGFβ1-Mediated Upregulation of Periostin Expression.
    Wnt5a, a member of the noncanonical Wnt proteins, is known to play important roles in the development of various organs and in postnatal cell functions. However, little is known about the effects of Wnt5a on human periodontal ligament (PDL) cells. In this study, we examined the localization and potential function of Wnt5a in PDL tissue. Immunohistochemical analysis revealed that Wnt5a was expressed predominantly in rat PDL tissue. Semi-quantitative reverse-transcription polymerase chain reaction and Western blotting analysis demonstrated that human PDL cells (HPDLCs) expressed Wnt5a and its receptors (Ror2, Fzd2, Fzd4, and Fzd5). Removal of occlusal pressure by extraction of opposing teeth decreased Wnt5a expression in rat PDL tissue, and the expression of Wnt5a and its receptors in HPDLCs was upregulated by exposure to mechanical stress. Stimulation with Wnt5a significantly enhanced the proliferation and migration of HPDLCs. Furthermore, Wnt5a suppressed osteoblastic differentiation of HPDLCs cultivated in osteogenic induction medium, while it significantly enhanced the expression of PDL-related genes, such as periostin, type-I collagen, and fibrillin-1 genes, and the production of collagen in HPDLCs cultivated in normal medium. Both knockdown of periostin gene expression by siRNA and inhibition of TGFβ1 function by neutralizing antibody suppressed the Wnt5a-induced PDL-related gene expression and collagen production in HPDLCs. Interestingly, in HPDLCs cultured with Wnt5a, TGFβ1 neutralizing antibody significantly suppressed periostin expression, while periostin siRNA had no effect on TGFβ1 expression. These results suggest that Wnt5a expressed in PDL tissue plays specific roles in inducing collagen production by PDL cells through TGFβ1-mediated upregulation of periostin expression.

    DOI: 10.1002/jcp.24950

  • Effect of CTGF/CCN2 on osteo/cementoblastic and fibroblastic differentiation of a human periodontal ligament stem/progenitor cell line. Reviewed International journal

    Asuka Yuda, Hidefumi Maeda, Shinsuke Fujii, Satoshi Monnouchi, Naohide Yamamoto, Naohisa Wada, Katsuaki Koori, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Akifumi Akamine

    Journal of cellular physiology   230 ( 1 )   150 - 9   2015.1

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    Appropriate mechanical loading during occlusion and mastication play an important role in maintaining the homeostasis of periodontal ligament (PDL) tissue. Connective tissue growth factor (CTGF/CCN2), a matricellular protein, is known to upregulate extracellular matrix production, including collagen in PDL tissue. However, the underlying mechanisms of CTGF/CCN2 in regulation of PDL tissue integrity remain unclear. In this study, we investigated the effect of CTGF/CCN2 on osteo/cementoblastic and fibroblastic differentiation of human PDL stem cells using the cell line 1-11. CTGF/CCN2 expression in rat PDL tissue and human PDL cells (HPDLCs) was confirmed immunohisto/cytochemically. Mechanical loading was found to increase gene expression and secretion of CTGF/CCN2 in HPDLCs. CTGF/CCN2 upregulated the proliferation and migration of 1-11 cells. Furthermore, increased bone/cementum-related gene expression in this cell line led to mineralization. In addition, combined treatment of 1-11 cells with CTGF/CCN2 and transforming growth factor-β1 (TGF-β1) significantly promoted type I collagen and fibronectin expression compared with that of TGF-β1 treatment alone. Thus, these data suggest the underlying biphasic effects of CTGF/CCN2 in 1-11 cells, inducible osteo/cementoblastic, and fibroblastic differentiation dependent on the environmental condition. CTGF/CCN2 may contribute to preservation of the structural integrity of PDL tissue, implying its potential use as a therapeutic agent for PDL regeneration.

    DOI: 10.1002/jcp.24693

  • Regeneration of the periodontium for preservation of the damaged tooth. Reviewed International journal

    Hidefumi Maeda, Atsushi Tomokiyo, Naohisa Wada, Katsuaki Koori, Giichiro Kawachi, Akifumi Akamine

    Histology and histopathology   29 ( 10 )   1249 - 62   2014.10

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    The population of the world grows every year, and life expectancy tends to increase. Thus, long-term preservation of teeth in aged individuals is an urgent issue. The main causes of tooth loss are well known to be periodontitis, caries, fractures, and orthodontic conditions. Although implant placement is a widely accepted treatment for tooth loss, most patients desire to preserve their own teeth. Many clinicians and researchers are therefore challenged to treat and preserve teeth that are irreversibly affected by deep caries, periodontitis, fractures, and trauma. Tissue engineering techniques are beneficial in addressing this issue; stem cells, signal molecules, and scaffolds are the main elements of such techniques. In this review, we describe these three elements with respect to their validation for regeneration of the periodontium and focus particularly on the potency of diverse scaffolds. In addition, we provide a short overview of the ongoing studies of 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate-tri-n-butyl-borane resin including calcium chloride or hydroxyapatite for periodontium regeneration.

    DOI: 10.14670/HH-29.1249

  • Effects of Activin A on the phenotypic properties of human periodontal ligament cells. Reviewed International journal

    Hideki Sugii, Hidefumi Maeda, Atsushi Tomokiyo, Naohide Yamamoto, Naohisa Wada, Katsuaki Koori, Daigaku Hasegawa, Sayuri Hamano, Asuka Yuda, Satoshi Monnouchi, Akifumi Akamine

    Bone   66   62 - 71   2014.9

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    Periodontal ligament (PDL) tissue plays an important role in tooth preservation by structurally maintaining the connection between the tooth root and the bone. The mechanisms involved in the healing and regeneration of damaged PDL tissue, caused by bacterial infection, caries and trauma, have been explored. Accumulating evidence suggests that Activin A, a member of the transforming growth factor-β (TGF-β) superfamily and a dimer of inhibinβa, contributes to tissue healing through cell proliferation, migration, and differentiation of various target cells. In bone, Activin A has been shown to exert an inhibitory effect on osteoblast maturation and mineralization. However, there have been no reports examining the expression and function of Activin A in human PDL cells (HPDLCs). Thus, we aimed to investigate the biological effects of Activin A on HPDLCs. Activin A was observed to be localized in HPDLCs and rat PDL tissue. When PDL tissue was surgically damaged, Activin A and IL-1β expression increased and the two proteins were shown to be co-localized around the lesion. HPDLCs treated with IL-1β or TNF-α also up-regulated the expression of the gene encoding inhibinβa. Activin A promoted chemotaxis, migration and proliferation of HPDLCs, and caused an increase in fibroblastic differentiation of these cells while down-regulating their osteoblastic differentiation. These osteoblastic inhibitory effects of Activin A, however, were only noted during the early phase of HPDLC osteoblastic differentiation, with later exposures having no effect on differentiation. Collectively, our results suggest that Activin A could be used as a therapeutic agent for healing and regenerating PDL tissue in response to disease, trauma or surgical reconstruction.

    DOI: 10.1016/j.bone.2014.05.021

  • The roles of calcium-sensing receptor and calcium channel in osteogenic differentiation of undifferentiated periodontal ligament cells. Reviewed International journal

    Katsuaki Koori, Hidefumi Maeda, Shinsuke Fujii, Atsushi Tomokiyo, Giichiro Kawachi, Daigaku Hasegawa, Sayuri Hamano, Hideki Sugii, Naohisa Wada, Akifumi Akamine

    Cell and tissue research   357 ( 3 )   707 - 18   2014.9

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    Elevated extracellular calcium has been shown to promote the differentiation of osteoblasts. However, the way that calcium affects the osteogenic differentiation of human periodontal ligament stem/progenitor cells (PDLSCs) remains unclear. Our aim has been to investigate the proliferation and osteogenic differentiation of a calcium-exposed human PDLSC line (cell line 1-17) that we have recently established and to elucidate the roles of the calcium-sensing receptor (CaSR) and L-type voltage-dependent calcium channel (L-VDCC) in this process. Proliferation activity was investigated by WST-1 assay, and gene and protein expression was examined by quantitative reverse transcriptase plus the polymerase chain reaction and immunostaining, respectively. Calcification assay was performed by von Kossa and Alizarin red staining. Treatment with 5 mM CaCl2 significantly induced proliferation, bone-related gene expression, and calcification in cell line 1-17. During culture with 5 mM CaCl2, this cell line up-regulated the gene expression of CaSR, which was reduced after 7 days. Simultaneous treatment with NPS2143, a CaSR inhibitor, and calcium significantly further increased bone-related gene expression and calcification as compared with CaCl2 exposure alone. The L-VDCC inhibitor, nifedipine, significantly suppressed osteogenic differentiation of cell line 1-17 treated with 5 mM CaCl2 and promoted the expression of CaSR, as compared with calcium treatment alone. Thus, elevated extracellular calcium promotes the proliferation and osteogenic differentiation of a PDLSC line. Antagonizing CaSR further enhances the effect of calcium on osteogenic differentiation, with CaSR expression being regulated by L-VDCC under extracellular calcium. Extracellular calcium might therefore modulate the osteogenic differentiation of PDLSCs through reciprocal adjustments of CaSR and L-VDCC.

    DOI: 10.1007/s00441-014-1918-5

  • Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells. Reviewed International journal

    Naohisa Wada, Hidefumi Maeda, Daigaku Hasegawa, Stan Gronthos, Peter Mark Bartold, Danijela Menicanin, Shinsuke Fujii, Shinichiro Yoshida, Atsushi Tomokiyo, Satoshi Monnouchi, Akifumi Akamine

    Stem cells and development   23 ( 18 )   2225 - 36   2014.9

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    Periodontal ligament stem cells (PDLSCs) have recently been proposed as a novel option in periodontal regenerative therapy. However, one of the issues is the difficulty of stably generating PDLSCs because of the variation of stem cell potential between donors. Here, we show that Semaphorin 3A (Sema3A) can induce mesenchymal-stem-like properties in human periodontal ligament (PDL) cells. Sema3A expression was specifically observed in the dental follicle during tooth development and in parts of mature PDL tissue in rodent tooth and periodontal tissue. Sema3A expression levels were found to be higher in multipotential human PDL cell clones compared with low-differentiation potential clones. Sema3A-overexpressing PDL cells exhibited an enhanced capacity to differentiate into both functional osteoblasts and adipocytes. Moreover, PDL cells treated with Sema3A only at the initiation of culture stimulated osteogenesis, while Sema3A treatment throughout the culture had no effect on osteogenic differentiation. Finally, Sema3A-overexpressing PDL cells upregulated the expression of embryonic stem cell markers (NANOG, OCT4, and E-cadherin) and mesenchymal stem cell markers (CD73, CD90, CD105, CD146, and CD166), and Sema3A promoted cell division activity of PDL cells. These results suggest that Sema3A may possess the function to convert PDL cells into mesenchymal-stem-like cells.

    DOI: 10.1089/scd.2013.0405

  • Expression and effects of epidermal growth factor on human periodontal ligament cells. Reviewed International journal

    Yoko Teramatsu, Hidefumi Maeda, Hideki Sugii, Atsushi Tomokiyo, Sayuri Hamano, Naohisa Wada, Asuka Yuda, Naohide Yamamoto, Katsuaki Koori, Akifumi Akamine

    Cell and tissue research   357 ( 3 )   633 - 43   2014.9

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    Repair of damaged periodontal ligament (PDL) tissue is an essential challenge in tooth preservation. Various researchers have attempted to develop efficient therapies for healing and regenerating PDL tissue based on tissue engineering methods focused on targeting signaling molecules in PDL stem cells and other mesenchymal stem cells. In this context, we investigated the expression of epidermal growth factor (EGF) in normal and surgically wounded PDL tissues and its effect on chemotaxis and expression of osteoinductive and angiogenic factors in human PDL cells (HPDLCs). EGF as well as EGF receptor (EGFR) expression was observed in HPDLCs and entire PDL tissue. In a PDL tissue-injured model of rat, EGF and IL-1β were found to be upregulated in a perilesional pattern. Interleukin-1β induced EGF expression in HPDLCs but not EGFR. It also increased transforming growth factor-α (TGF-α) and heparin-binding EGF-like growth factor (HB-EGF) expression. Transwell assays demonstrated the chemotactic activity of EGF on HPDLCs. In addition, EGF treatment significantly induced secretion of bone morphogenetic protein 2 and vascular endothelial growth factor, and gene expression of interleukin-8 (IL-8), and early growth response-1 and -2 (EGR-1/2). Human umbilical vein endothelial cells developed well-formed tube networks when cultured with the supernatant of EGF-treated HPDLCs. These results indicated that EGF upregulated under inflammatory conditions plays roles in the repair of wounded PDL tissue, suggesting its function as a prospective agent to allow the healing and regeneration of this tissue.

    DOI: 10.1007/s00441-014-1877-x

  • Effect of CTGF/CCN2 on osteo/cementoblastic and fibroblastic differentiation of a human periodontal ligament stem/progenitor cell line. Reviewed International journal

    Asuka Yuda, Hidefumi Maeda, Fujii Shinsuke, Monnouchi Satoshi, Naohide Yamamoto, Naohisa Wada, Koori Katsuaki, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Akifumi Akamine

    J Cell Physiol   2014.6

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  • Mechanical induction of interleukin-11 regulates osteoblastic/cementoblastic differentiation of human periodontal ligament stem/progenitor cells. Reviewed International journal

    Monnouchi Satoshi, Hidefumi Maeda, Asuka Yuda, Sayuri Hamano, Naohisa Wada, Atsushi Tomokiyo, Koori Katsuaki, Hideki Sugii, Suguru Serita, Akifumi Akamine

    J Periodontal Res   2014.6

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  • Effects of Activin A on the phenotypic properties of human periodontal ligament cells. Reviewed International journal

    Hideki Sugii, Hidefumi Maeda, Atsushi Tomokiyo, Naohide Yamamoto, Naohisa Wada, Koori Katsuaki, Daigaku Hasegawa, Sayuri Hamano, Asuka Yuda, Monnouchi Satoshi, Akifumi Akamine

    Bone   2014.6

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  • The roles of calcium-sensing receptor and calcium channel in osteogenic differentiation of undifferentiated periodontal ligament cells. Reviewed International journal

    Koori Katsuaki, Hidefumi Maeda, Fujii Shinsuke, Atsushi Tomokiyo, G Kawachi, Daigaku Hasegawa, Sayuri Hamano, Hideki Sugii, Naohisa Wada, Akifumi Akamine

    Cell Tissue Res   2014.5

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  • Periodontal-ligament-derived stem cells exhibit the capacity for long-term survival, self-renewal, and regeneration of multiple tissue types in vivo. Reviewed International journal

    Danijela Menicanin, Krzysztof Marek Mrozik, Naohisa Wada, Victor Marino, Songtao Shi, P Mark Bartold, Stan Gronthos

    Stem cells and development   23 ( 9 )   1001 - 11   2014.5

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    Primary periodontal ligament stem cells (PDLSCs) are known to possess multidifferentiation potential and exhibit an immunophenotype similar to that described for bone-marrow-derived mesenchymal stem cells. In the present study, bromo-deoxyuridine (BrdU)-labeled ovine PDLSCs implanted into immunodeficient mice survived after 8 weeks post-transplantation and exhibited the capacity to form bone/cementum-like mineralized tissue, ligament structures similar to Sharpey's fibers with an associated vasculature. To evaluate self-renewal potential, PDLSCs were recovered from harvested primary transplants 8 weeks post-transplantation that exhibit an immunophenotype and multipotential capacity comparable to primary PDLSCs. The re-derived PDLSCs isolated from primary transplants were implanted into secondary ectopic xenogeneic transplants. Histomorphological analysis demonstrated that four out of six donor re-derived PDLSC populations displayed a capacity to survive and form fibrous ligament structures and mineralized tissues associated with vasculature in vivo, although at diminished levels in comparison to primary PDLSCs. Further, the capacity for long-term survival and the potential role of PDLSCs in dental tissue regeneration were determined using an ovine preclinical periodontal defect model. Autologous ex vivo-expanded PDLSCs that were prelabeled with BrdU were seeded onto Gelfoam(®) scaffolds and then transplanted into fenestration defects surgically created in the periodontium of the second premolars. Histological assessment at 8 weeks post-implantation revealed surviving BrdU-positive PDLSCs associated with regenerated periodontium-related tissues, including cementum and bone-like structures. This is the first report to demonstrate the self-renewal capacity of PDLSCs using serial xenogeneic transplants and provides evidence of the long-term survival and tissue contribution of autologous PDLSCs in a preclinical periodontal defect model.

    DOI: 10.1089/scd.2013.0490

  • Expression and effects of epidermal growth factor on human periodontal ligament cells. Reviewed International journal

    Yoko Teramatsu, Hidefumi Maeda, Hideki Sugii, Atsushi Tomokiyo, Sayuri Hamano, Naohisa Wada, Asuka Yuda, Naohide Yamamoto, Katsuaki Koori, Akifumi Akamine

    Cell Tissue Res   2014.5

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  • Periodontal tissue engineering: defining the triad. Reviewed

    Maeda H, Fujii S, Tomokiyo A, Wada N, Akamine A

    The International journal of oral & maxillofacial implants   28 ( 6 )   e461 - 71   2013.11

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    Periodontal tissue engineering: defining the triad.

    DOI: 10.11607/jomi.te26

  • Regeneration of periodontal tissues using allogeneic periodontal ligament stem cells in an ovine model. Reviewed International journal

    Krzysztof Marek Mrozik, Naohisa Wada, Victor Marino, Ward Richter, Songtao Shi, Donna L Wheeler, Stan Gronthos, P Mark Bartold

    Regenerative medicine   8 ( 6 )   711 - 23   2013.11

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    AIM: To investigate the capacity of allogeneic periodontal ligament stem cells (PDLSCs) to regenerate periodontal tissues using an ovine periodontal defect model. MATERIALS & METHODS: Surgically created zero-wall dehiscence periodontal defects created in Merino sheep were filled with 1 × 10(7) allogeneic PDLSCs attached to Gelfoam(®), Gelfoam alone or left untreated. After 4 weeks, histological analysis was performed to assess periodontal regeneration. RESULTS: Allogeneic PDLSCs were well tolerated by recipient animals. The mean area of new alveolar bone was significantly greater in the PDLSC + Gelfoam treatment group compared with the defect-alone group. The PDLSC + Gelfoam and Gelfoam-only treatment groups displayed significantly greater length of new cementum and percentage of cementum regrowth compared with the defect-alone group. New Sharpey's fibers were generally more organized and significantly thicker within the PDLSC + Gelfoam treatment group. The PDLSC + Gelfoam treatment group also showed a trend of increased Sharpey's fiber attachment length compared with the Gelfoam-only and defect-alone groups. CONCLUSION: These studies support the potential use of allogeneic PDLSC preparations as viable therapies for periodontal regeneration in the clinical setting.

    DOI: 10.2217/rme.13.66

  • Immunomodulatory effects of stem cells. Reviewed International journal

    Naohisa Wada, Stan Gronthos, P Mark Bartold

    Periodontology 2000   63 ( 1 )   198 - 216   2013.10

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    Adult-derived mesenchymal stem cells have received considerable attention over the past two decades for their potential use in tissue engineering, principally because of their potential to differentiate into multiple stromal-cell lineages. Recently, the immunomodulatory properties of mesenchymal stem cells have attracted interest as a unique property of these cells that may be harnessed for novel therapeutic approaches in immune-mediated diseases. Mesenchymal stem cells have been shown to inhibit the proliferation of activated T-cells both in vitro and in vivo but to stimulate T-regulatory cell proliferation. Mesenchymal stem cells are also known to be weakly immunogenic and to exert immunosuppressive effects on B-cells, natural killer cells, dendritic cells and neutrophils through various mechanisms. Furthermore, intravenous administration of allogeneic mesenchymal stem cells has shown a marked suppression of host immune reactions in preclinical animal models of large-organ transplant rejection and in various autoimmune- and inflammatory-based diseases. Some clinical trials utilizing human mesenchymal stem cells have also produced promising outcomes in patients with graft-vs.-host disease and autoimmune diseases. Mesenchymal stem cells identified from various dental tissues, including periodontal ligament stem cells, also possess multipotent and immunomodulatory properties. Hence, dental mesenchymal stem cells may represent an alternate cell source, not only for tissue regeneration but also as therapies for autoimmune- and inflammatory-mediated diseases. These findings have elicited interest in dental tissue mesenchymal stem cells as alternative cell sources for modulating alloreactivity during tissue regeneration following transplantation into human leukocyte antigen-mismatched donors. To examine this potential in periodontal regeneration, future work will need to assess the capacity of allogeneic periodontal ligament stem cells to regenerate periodontal ligament in animal models of periodontal disease. The present review describes the immunosuppressive effects of mesenchymal stem cells on various types of immune cells, the potential mechanisms through which they exert their mode of action and the preclinical animal studies and human clinical trials that have utilized mesenchymal stem cells, including those populations originating from dental structures.

    DOI: 10.1111/prd.12024

  • CCNファミリー研究のメルティングポット CTGF/CCN2が未分化なヒト歯根膜細胞株の骨芽細胞様分化に及ぼす影響

    祐田 明香, 前田 英史, 藤井 慎介, 門野内 聡, 山本 直秀, 和田 尚久, 友清 淳, 郡 勝明, 濱野 さゆり, 赤峰 昭文

    Journal of Oral Biosciences Supplement   2013   96 - 96   2013.9

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  • Exposure to transforming growth factor-β1 after basic fibroblast growth factor promotes the fibroblastic differentiation of human periodontal ligament stem/progenitor cell lines. Reviewed International journal

    Kono Kiyomi, Hidefumi Maeda, Fujii Shinsuke, Atsushi Tomokiyo, Yamamoto N, Naohisa Wada, Monnouchi Satoshi, Teramatsu Y, Hamano S, Koori K, Akifumi Akamine

    Cell Tissue Res   352 ( 2 )   249 - 263   2013.5

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  • Exposure to transforming growth factor-β1 after basic fibroblast growth factor promotes the fibroblastic differentiation of human periodontal ligament stem/progenitor cell lines. Reviewed International journal

    Kono K, Maeda H, Fujii S, Tomokiyo A, Yamamoto N, Wada N, Monnouchi S, Teramatsu Y, Hamano S, Koori K, Akamine A

    Cell and tissue research   352 ( 2 )   249 - 63   2013.5

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    Exposure to transforming growth factor-β1 after basic fibroblast growth factor promotes the fibroblastic differentiation of human periodontal ligament stem/progenitor cell lines.
    Basic fibroblast growth factor (bFGF) is a cytokine that promotes the regeneration of the periodontium, the specialized tissues supporting the teeth. bFGF, does not, however, induce the synthesis of smooth muscle actin alpha 2 (ACTA2), type I collagen (COL1), or COL3, which are principal molecules in periodontal ligament (PDL) tissue, a component of the periodontium. We have suggested the feasibility of using transforming growth factor-β1 (TGFβ1) to induce fibroblastic differentiation of PDL stem/progenitor cells (PDLSCs). Here, we investigated the effect of the subsequent application of TGFβ1 after bFGF (bFGF/TGFβ1) on the differentiation of PDLSCs into fibroblastic cells. We first confirmed the expression of bFGF and TGFβ1 in rat PDL tissue and primary human PDL cells. Receptors for both bFGF and TGFβ1 were expressed in the human PDLSC lines 1-11 and 1-17. Exposure to bFGF for 2 days promoted vascular endothelial growth factor gene and protein expression in both cell lines and down-regulated the expression of ACTA2, COL1, and COL3 mRNA in both cell lines and the gene fibrillin 1 (FBN1) in cell line 1-11 alone. Furthermore, bFGF stimulated cell proliferation of these cell lines and significantly increased the number of cells in phase G2/M in the cell lines. Exposure to TGFβ1 for 2 days induced gene expression of ACTA2 and COL1 in both cell lines and FBN1 in cell line 1-11 alone. BFGF/TGFβ1 treatment significantly up-regulated ACTA2, COL1, and FBN1 expression as compared with the group treated with bFGF alone or the untreated control. This method might thus be useful for accelerating the generation and regeneration of functional periodontium.

    DOI: 10.1007/s00441-012-1543-0

  • Adenosine blocks aminopterin-induced suppression of osteoclast differentiation. Reviewed International journal

    Teramachi J, Kukita A, Qu P, naohisa wada, Li YJ, Toshio Kukita

    J Bone Miner Metab   31 ( 1 )   64 - 70   2013.1

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  • Adenosine blocks aminopterin-induced suppression of osteoclast differentiation. Reviewed

    Junpei Teramachi, Akiko Kukita, Pengfei Qu, Naohisa Wada, Yin-Ji Li, Seiji Nakamura, Toshio Kukita

    Journal of bone and mineral metabolism   31 ( 1 )   64 - 70   2013.1

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    To search cell surface molecules involved in the regulation of osteoclastogenesis, especially in fusion process, it is one powerful approach to obtain monoclonal antibodies bearing ability to block formation of multinucleated osteoclasts. Ideally, direct bio-assay of hybridoma supernatants is quite convenient to screen monoclonal antibodies of interest from numerous culture wells. However, addition of hybridoma supernatant containing hypoxanthine-aminopterin-thymidine (HAT), components of the selection medium, to whole bone marrow cultures strikingly suppressed osteoclastogenesis. Here we clarified aminopterin is the responsible component in HAT medium to inhibit osteoclastogenesis. Methotrexate (MTX), mono-methylated aminopterin, showed similar suppressive effect on osteoclastogenesis. When bone marrow cells were cultured in the presence of all nucleosides, aminopterin and MTX-induced suppression of osteoclastogenesis was abrogated. Among four nucleosides only adenosine canceled aminopterin-induced suppression of osteoclastogenesis. Direct bio-assay of hybridoma supernatant containing HAT selection medium is now available to screen monoclonal antibodies if adenosine-containing culture medium was utilized for evaluating osteoclastogenesis.

    DOI: 10.1007/s00774-012-0388-7

  • Synthesis of novel triplet drugs with 1,3,5-trioxazatriquinane skeletons and their pharmacologies. 3: synthesis of novel triplet drugs with the bis(epoxymethano) or bis(dimethylepoxymethano) structure (double-capped triplet). Reviewed International journal

    Naohisa Wada, Hideaki Fujii, Koji Koyano, Shigeto Hirayama, Takashi Iwai, Toru Nemoto, Hiroshi Nagase

    Bioorganic & medicinal chemistry letters   22 ( 24 )   7551 - 4   2012.12

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    Novel double-capped triplet drugs, which have one pharmacophore unit and two epoxymethano or dimethylepoxymethano structures (termed cap or diMe-cap structures, respectively) were synthesized. Key intermediate oxazoline 16 derived from acetone enabled the effective synthesis of double-capped triplets. SYK-134 (7a) and SYK-135 (8a) with N-cyclopropylmethyl substituent and cap structures showed selectivities for the κ opioid receptor. On the other hand, the N-Me series exhibited selectivities for the μ opioid receptor. The double-capped triplet drugs with diMe-cap structures preferred the μ receptor independently of their N-substituents. SYK-385 (19b), one of the μ-selective double-capped triplet drugs, showed the highest selectivity for the μ receptor among the reported μ-selective nonpeptide ligands.

    DOI: 10.1016/j.bmcl.2012.10.023

  • Alternation of extracellular matrix remodeling and apoptosis by activation of the aryl hydrocarbon receptor pathway in human periodontal ligament cells. Reviewed International journal

    Atsushi Tomokiyo, Hidefumi Maeda, Shinsuke Fujii, Satoshi Monnouchi, Naohisa Wada, Kiyomi Hori, Katsuaki Koori, Naohide Yamamoto, Yoko Teramatsu, Akifumi Akamine

    Journal of cellular biochemistry   113 ( 10 )   3093 - 103   2012.10

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    It is well known that the aryl hydrocarbon receptor (AhR) is involved in the toxicity of halogenated aromatic hydrocarbons (HAH) and polycyclic aromatic hydrocarbons (PAH). Recent experiments have shown the induction of impaired tooth and hard-tissue formation by AhR pathway activation, however, the effect on periodontal ligament (PDL) tissue remains unclear. Here, we investigated the effects of benzo(a)pyrene (BaP), a member of PAH, on the extracellular matrix (ECM) remodeling-related molecules, collagen type I (COL-I), matrix metalloproteinase-1 (MMP-1), alpha-smooth muscle actin (α-SMA) expression, and apoptosis in two different human periodontal ligament cells (HPDLCs). The transduction of AhR from the cytoplasm to the nucleus and the increase of AhR-responsive genes; that is, cytochrome P450 1A1 (CYP1A1), cytochrome P450 1B1 (CYP1B1), and aryl-hydrocarbon receptor repressor (AhRR), expression was induced by BaP exposure in both HPDLCs. BaP treatment significantly enhanced MMP-1 mRNA expression and MMP-1 protein production, while markedly suppressing COL-I and a-SMA mRNA expression in both HPDLCs. Furthermore, these BaP-treated HPDLCs fell into apoptotic cell death as evidenced by induction in annexin V and caspase-3/7 staining and reduction of total cell number and Bcl-2 mRNA expression. Thus, BaP exposure altered the expression of ECM-related molecules and induced apoptosis in HPDLCs through activation of the AhR pathway. Overactivity of the AhR pathway may induce an inappropriate turnover of PDL tissue via disordered ECM remodeling and apoptosis in PDL cells.

    DOI: 10.1002/jcb.24186

  • Expression and effects of glial cell line-derived neurotrophic factor on periodontal ligament cells. Reviewed International journal

    Naohide Yamamoto, Hidefumi Maeda, Atsushi Tomokiyo, Shinsuke Fujii, Naohisa Wada, Satoshi Monnouchi, Kiyomi Kono, Katsuaki Koori, Yoko Teramatsu, Akifumi Akamine

    Journal of clinical periodontology   39 ( 6 )   556 - 64   2012.6

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    AIM: To investigate Glial cell line-derived neurotrophic factor (GDNF) expression in normal and wounded rat periodontal ligament (PDL) and the effects of GDNF on human PDL cells (HPDLCs) migration and extracellular matrix expression in HPDLCs. MATERIAL AND METHODS: The expression of GDNF and GDNF receptors was examined by immunocyto/histochemical analyses. Gene expression in HPDLCs treated with GDNF, interleukin-1 beta (IL-1β), or tumour necrosis factor-alpha (TNF-α) was quantified by quantitative RT-PCR (qRT-PCR). In addition, we examined the migratory effect of GDNF on HPDLCs. RESULTS: GDNF was expressed in normal rat PDL and cultured HPDLCs. HPDLCs also expressed GDNF receptors. In wounded rat PDL, GDNF expression was up-regulated. QRT-PCR analysis revealed that IL-1β and TNF-α significantly increased the expression of GDNF in HPDLCs. Furthermore, GDNF induced migration of HPDLCs, which was blocked by pre-treatment with the peptide including Arg-Gly-Asp (RGD) sequence, or neutralizing antibodies against integrin αVβ3 or GDNF. Also, GDNF up-regulated expression of bone sialoprotein (BSP) and fibronectin in HPDLCs. CONCLUSIONS: GDNF expression is increased in rat wounded PDL tissue and HPDLCs treated with pro-inflammatory cytokines. GDNF enhances the expression of BSP and fibronectin, and migration in an RGD-dependent manner via the integrin αVβ3. These findings suggest that GDNF may contribute to wound healing in PDL tissue.

    DOI: 10.1111/j.1600-051X.2012.01881.x

  • Expression and effects of glial cell line-derived neurotrophic factor on periodontal ligament cells. Reviewed International journal

    Yamamoto N, Maeda H, Tomokiyo A, Fujii S, Wada N, Monnouchi S, Kono K, Koori K, Teramatsu Y, Akamine A

    J Clin Periodontol   39 ( 6 )   2012.6

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  • A multipotent clonal human periodontal ligament cell line with neural crest cell phenotypes promotes neurocytic differentiation, migration, and survival. Reviewed International journal

    Atsushi Tomokiyo, Hidefumi Maeda, Shinsuke Fujii, Satoshi Monnouchi, Naohisa Wada, Kiyomi Kono, Naohide Yamamoto, Katsuaki Koori, Yoko Teramatsu, Akifumi Akamine

    Journal of cellular physiology   227 ( 5 )   2040 - 50   2012.5

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    Repair of injured peripheral nerve is thought to play important roles in tissue homeostasis and regeneration. Recent experiments have demonstrated enhanced functional recovery of damaged neurons by some types of somatic stem cells. It remains unclear, however, if periodontal ligament (PDL) stem cells possess such functions. We recently developed a multipotent clonal human PDL cell line, termed cell line 1-17. Here, we investigated the effects of this cell line on neurocytic differentiation, migration, and survival. This cell line expressed the neural crest cell marker genes Slug, SOX10, Nestin, p75NTR, and CD49d and mesenchymal stem cell-related markers CD13, CD29, CD44, CD71, CD90, CD105, and CD166. Rat adrenal pheochromocytoma cells (PC12 cells) underwent neurocytic differentiation when co-cultured with cell line 1-17 or in conditioned medium from cell line 1-17 (1-17CM). ELISA analysis revealed that 1-17CM contained approximately 50 pg/ml nerve growth factor (NGF). Cell line 1-17-induced migration of PC12 cells, which was inhibited by a neutralizing antibody against NGF. Furthermore, 1-17CM exerted antiapoptotic effects on differentiated PC12 cells as evidenced by inhibition of neurite retraction, reduction in annexin V and caspase-3/7 staining, and induction of Bcl-2 and Bcl-xL mRNA expression. Thus, cell line 1-17 promoted neurocytic differentiation, migration, and survival through secretion of NGF and possibly synergistic factors. PDL stem cells may play a role in peripheral nerve reinnervation during PDL regeneration.

    DOI: 10.1002/jcp.22933

  • Alternation of extracellular matrix remodeling and apoptosis by activation of the aryl hydrocarbon receptor pathway in human periodontal ligament cells. Reviewed International journal

    Atsushi Tomokiyo, Hidefumi Maeda, Fujii Shinsuke, Monnouchi Satoshi, naohisa wada, Kono Kiyomi, Koori K, Yamamoto N, Teramatsu Y, Akifumi Akamine

    J Cell Biochem   113 ( 10 )   3093 - 3103   2012.5

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  • A multipotent clonal human periodontal ligament cell line with neural crest cell phenotypes promotes neurocytic differentiation, migration, and survival. Reviewed International journal

    naohisa wada, Hidefumi Maeda, Kono Kiyomi, Atsushi Tomokiyo, Monnouchi Satoshi, Katsuaki Koori, Naohide Yamamoto, Yoko Teramatsu, Akifumi Akamine

    J Cell Physiol   227 ( 5 )   2040 - 2050   2012.5

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  • 油症患者における歯周疾患ならびに口腔内色素沈着の疫学的調査(第八報)

    橋口勇,吉嶺嘉人,前田英史,後藤康治,和田尚久,藤井慎介,友清淳,齋籐桐枝,門野内聡,河野清美,奥村英彦,赤峰昭文

    福岡医誌   102 ( 4 )   2011.8

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  • Promise of periodontal ligament stem cells in regeneration of periodontium. Reviewed International journal

    Hidefumi Maeda, Atsushi Tomokiyo, Shinsuke Fujii, Naohisa Wada, Akifumi Akamine

    Stem cell research & therapy   2 ( 4 )   33 - 33   2011.7

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    A great number of patients around the world experience tooth loss that is attributed to irretrievable damage of the periodontium caused by deep caries, severe periodontal diseases or irreversible trauma. The periodontium is a complex tissue composed mainly of two soft tissues and two hard tissues; the former includes the periodontal ligament (PDL) tissue and gingival tissue, and the latter includes alveolar bone and cementum covering the tooth root. Tissue engineering techniques are therefore required for regeneration of these tissues. In particular, PDL is a dynamic connective tissue that is subjected to continual adaptation to maintain tissue size and width, as well as structural integrity, including ligament fibers and bone modeling. PDL tissue is central in the periodontium to retain the tooth in the bone socket, and is currently recognized to include somatic mesenchymal stem cells that could reconstruct the periodontium. However, successful treatment using these stem cells to regenerate the periodontium efficiently has not yet been developed. In the present article, we discuss the contemporary standpoints and approaches for these stem cells in the field of regenerative medicine in dentistry.

    DOI: 10.1186/scrt74

  • An in vitro evaluation of two resin-based sealers on proliferation and differentiation of human periodontal ligament cells. Reviewed International journal

    Maeda H, Tomokiyo A, Koori K, Monnouchi S, Fujii S, Wada N, Kono K, Yamamoto N, Saito T, Akamine A

    Int Endod J   44 ( 5 )   2011.7

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  • An in vitro evaluation of two resin-based sealers on proliferation and differentiation of human periodontal ligament cells. Reviewed

    Maeda H, Tomokiyo A, Koori K, Monnouchi S, Fujii S, Wada N, Kono K, Yamamoto N, Saito T, Akamine A

    Int Endod J   44 ( 5 )   425 - 431   2011.5

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    An in vitro evaluation of two resin-based sealers on proliferation and differentiation of human periodontal ligament cells.

  • Adenosine abolishes MTX-induced suppression of osteoclastogenesis and inflammatory bone destruction in adjuvant-induced arthritis. Reviewed International journal

    Junpei Teramachi, Akiko Kukita, Yin-Ji Li, Yuki Ushijima, Hiroshi Ohkuma, Naohisa Wada, Toshiyuki Watanabe, Seiji Nakamura, Toshio Kukita

    Laboratory investigation; a journal of technical methods and pathology   91 ( 5 )   719 - 31   2011.5

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    Methotrexate (MTX) is widely utilized for the treatment of patients with rheumatoid arthritis (RA); however, recent observation of the MTX-resistant patients proposed some difficulty in MTX-dependent therapeutic approach for RA. To access cellular events related to MTX resistance in RA in respect to inflammatory bone destruction, we investigated on an involvement of the potent inflammatory mediator adenosine in the regulation of osteoclastogenesis and inflammatory bone destruction. In rats with adjuvant-induced arthritis (AA rats), MTX efficiently suppressed bone destruction when it was administrated within 3 days after adjuvant injection, while it could not suppress inflammatory bone destruction if MTX was injected at the time of onset of inflammation (at day 10 after adjuvant injection). Time-course change in the level of plasma adenosine of AA rats was estimated by use of high-performance liquid chromatography and elucidated that adenosine level was markedly elevated till 10 days after adjuvant injection. In vitro bone marrow culture system for evaluating osteoclastogenesis, MTX markedly suppressed osteoclastogenesis in a stromal cell-dependent manner. This MTX-induced suppression of osteoclastogenesis was abrogated by the addition of adenosine. MTX suppressed the expression of mRNA for the receptor activator NF-κB ligand (RANKL), but it did not suppress the expression of osteoprotegerin (OPG). The addition of MTX and adenosine together markedly suppressed the level of OPG expression. Abolishment of MTX action by adenosine was significantly blocked by MRS1754, a highly selective antagonist for the A(2b) adenosine receptor (A(2b)AR), but not by caffeine, an antagonist for A₁, A(2a), A₃ AR (A₁AR, A(2a)AR, and A₃AR), which suggests that adenosine acts through A(2b)AR. Immunohistochemical studies showed abundant expression of A(2b)AR in cells localized in the bone-bone marrow boundary of the distal tibia in AA rats but not in control rats. When adenosine was injected in the ankle joints of MTX-treated AA rats, the suppressive effects of MTX on bone destruction was abolished. The current data therefore suggest that upregulation of adenosine production abolished the suppressive effect of MTX on osteoclastic bone destruction. Involvement of the adenosine-A(2b)AR system may explain MTX resistance in RA.

    DOI: 10.1038/labinvest.2011.9

  • レジンシーラーの細胞親和性について-スーパーボンド根充シーラーとAH Plusとの比較-. Reviewed

    前田英史、友清淳、郡勝明、藤井慎介、門野内聡、和田尚久、河野清美、山本直秀、寺松陽子、赤峰昭文

    日本歯内療法学会誌   32 ( 2 )   2011.5

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  • Adenosine abolishes MTX-induced suppression of osteoclastogenesis and inflammatory bone destruction in adjuvant-induced arthritis Reviewed International journal

    Teramachi J, Kukita A, Li YJ, Ushijima Y, Ohkuma H, Wada N, Watanabe T, Nakamura S, Kukita T

    Lab Invest   91 ( 5 )   2011.5

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  • 【油症とPCB及びダイオキシン関連化合物に関する研究 報告集 第23集】油症患者における歯周疾患ならびに口腔内色素沈着の疫学的調査(第八報)

    橋口 勇, 吉嶺 嘉人, 前田 英史, 後藤 康治, 和田 尚久, 藤井 慎介, 友清 淳, 齋籐 桐枝, 門野内 聡, 河野 清美, 奥村 英彦, 赤峰 昭文

    福岡医学雑誌   102 ( 4 )   75 - 80   2011.4

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    油症患者における歯周疾患や口腔内色素沈着の罹患状況と経年的変化を把握するために、平成22年度の福岡県油症一斉検診時に歯科を受診した油症認定患者122名(男性56名、女性66名)を対象に、問診、視診やX線診(パントモグラフ)と同時に歯周ポケット診査を行った。主訴としては歯周組織炎や歯髄炎が多かった。深さ3mm以上の歯周ポケットを1歯でも有している患者は、被験者117名中104名(88.9%)と高い割合を示した。3mm以上の歯周ポケットを有する歯牙は総被検歯551歯中314歯(57.0%)であったが、その殆どは深さ4mm未満であった。口腔内色素沈着を有している患者は63名(51.6%)で、部位としては歯肉の色素沈着が最も多かった。女性より男性の発現率が高く、また70歳未満の患者に多く認められた。

  • [An epidemiologic examination on the prevalence of the periodontal diseases and oral pigmentation in Yusho patients in 2010]. Reviewed

    Isamu Hashiguchi, Yoshito Yoshimine, Hidefumi Maeda, Yasuharu Gotou, Naohisa Wada, Shinsuke Fujii, Atsushi Tomokiyo, Kirie Saito, Satoshi Monnouchi, Kiyomi Kouno, Hidehiko Okumura, Akifumi Akamine

    Fukuoka igaku zasshi = Hukuoka acta medica   102 ( 4 )   75 - 80   2011.4

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    An epidemiologic examination was carried out to reveal the prevalence of the periodontal diseases and oral pigmentation in patients with Yusho in 2010. The results obtained were as follows. 1) Yusho patients complained of tooth pain and periodontal diseases such as gingival swelling, but not of oral pigmentation. 2) 104 patients out of 117 patients with Yusho, who were measured periodontal pocket depth according to Ramfjord' methods, had at least one tooth with periodontal pocket deeper than 3 mm. Similarly, 314 teeth out of a total 551 examined teeth showed a periodontal pocket with more than 3 mm in depth. However, it was determined that 57 teeth had a periodontal pocket deeper than 4 mm. 3) Oral pigmentation was observed in 63 patients out of 122 patients with Yusho. In this study, gingival pigmentation was most predominant among oral pigmentation. The prevalence of oral pigmentation in male patients seemed to be somewhat higher than that in female patients. In addition, the prevalence of oral pigmentation tended to be higher in patients under seventy years old than patients beyond the age of seventy. These results indicated that PCB-related compounds may be responsible for the higher prevalence of both periodontal diseases and oral pigmentation.

    DOI: 10.15017/19724

  • Human foreskin fibroblasts exert immunomodulatory properties by a different mechanism to bone marrow stromal/stem cells. Reviewed International journal

    Naohisa Wada, Peter Mark Bartold, Stan Gronthos

    Stem cells and development   20 ( 4 )   647 - 59   2011.4

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    Human bone marrow mesenchymal stromal/stem cells (BMSCs) have been reported to possess immunomodulatory functions with the capacity to suppress immune reactions partly mediated by immunosuppressive factors, indoleamine 2,3-dioxygenase and nitric oxide, and suggested to be potentially applicable for therapeutic use. More recently, other fibroblast-like cells have been reported to possess similar properties. Here we demonstrate that human foreskin fibroblasts (HFFs) express an MSC-like immunophenotype and possess immunosuppressive properties similar to BMSCs but lack the capacity for osteogenic and adipogenic differentiation. HFFs suppressed human peripheral blood mononuclear cells (PBMC) proliferation stimulated with mitogen or in an allogeneic mixed lymphocyte reaction comparable to BMSCs. However, HFFs showed undetectable levels of indoleamine 2,3-dioxygenase and inducible nitric oxide synthase expression, in contrast to BMSCs when cocultured with activated PBMCs. To identify HFF specific immunosuppressive factors, we performed array profiling of common cytokines expressed by HFFs and BMSCs alone or when cocultured with activated PBMCs. Real-time polymerase chain reaction analysis confirmed that multiple factors were upregulated in HFFs cocultured with activated PBMCs compared with HFFs alone or BMSCs cultured under the same conditions. Functional assays identified interferon-α as the major immunosuppressive mediator expressed by HFFs. These results suggest that the HFFs possess immunosuppressive properties, which are mediated by alternate mechanisms to that reported for BMSCs.

    DOI: 10.1089/scd.2010.0246

  • Effects of TGF-β1 on the proliferation and differentiation of human periodontal ligament cells and a human periodontal ligament stem/progenitor cell line. Reviewed International journal

    Fujii S, Maeda H, Tomokiyo A, Monnouchi S, Hori K, Wada N, Akamine A

    Cell and tissue research   342 ( 2 )   233 - 42   2010.11

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    Effects of TGF-β1 on the proliferation and differentiation of human periodontal ligament cells and a human periodontal ligament stem/progenitor cell line.
    Periodontal ligament (PDL) is a specialized connective tissue that influences the lifespan of the tooth. Transforming growth factor-β1 (TGF-β1) is a multifunctional cytokine, but little is known about the effects of TGF-β1 on PDL cells. Our aim has been to demonstrate the expression of TGF-β1 in rat PDL tissues and to evaluate its effects on the proliferation and gene expression in human PDL cells (HPLCs) and a human PDL stem/progenitor cell line, line 1-11, that we have recently developed. The expression of TGF-β1 in the entire PDL tissue was confirmed immunohistochemically, and both HPLCs and cell line 1-11 expressed mRNA from the TGF-β1, TGF-β type I receptor, and TGF-β type II receptor genes. Although exogenous TGF-β1 stimulated the proliferation of HPLCs, it did not upregulate the expression of alpha-smooth muscle actin (α-SMA), type I collagen (Col I), or fibrillin-1 (FBN1) mRNA or of α-SMA protein in HPLCs, whereas expression for these genes was attenuated by an anti-TGF-β1 neutralizing antibody. In contrast, exogenous TGF-β1 reduced the proliferation of cell line 1-11, although it upregulated the expression of α-SMA, Col I, and FBN1 mRNA and of α-SMA protein in this cell line. In addition, interleukin-1 beta stimulation significantly reduced the expression of TGF-β1 mRNA and protein in HPLCs. Thus, TGF-β1 seems to play an important role in inducing fibroblastic differentiation of PDL stem/progenitor cells and in maintaining the PDL apparatus under physiological conditions.

    DOI: 10.1007/s00441-010-1037-x

  • Effects of TGF-β1 on the proliferation and differentiation of human periodontal ligament cells and a human periodontal ligament stem/progenitor cell line Reviewed International journal

    Fujii S, Maeda H, Tomokiyo A, Monnouchi S, Hori K, Wada N, Akamine A

    Cell Tissue Res   2010.10

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  • スーパーボンドシーラーの特性について ヒト歯根膜細胞の増殖と分化に与える影響

    前田 英史, 友清 淳, 郡 勝明, 藤井 慎介, 門野内 聡, 安田 善之, 山本 直秀, 堀 清美, 和田 尚久, 斎藤 隆史, 赤峰 昭文

    日本歯内療法学会学術大会プログラム・抄録集   31回   94 - 94   2010.5

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  • Mineral trioxide aggregate induces bone morphogenetic protein-2 expression and calcification in human periodontal ligament cells. Reviewed International journal

    Hidefumi Maeda, Tsuguhisa Nakano, Atsushi Tomokiyo, Shinsuke Fujii, Naohisa Wada, Satoshi Monnouchi, Kiyomi Hori, Akifumi Akamine

    Journal of endodontics   36 ( 4 )   647 - 52   2010.4

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    INTRODUCTION: Mineral trioxide aggregate (MTA) is a therapeutic, endodontic repair material that is reported to exhibit calcified tissue-conductive activity although the mechanisms remain unclear. We hypothesize that the dissolution of calcium from MTA into the surrounding environment may play an important role in the osteoblastic/cementoblastic differentiation of human periodontal ligament cells (HPLCs). METHODS: Two populations of HPLCs were obtained from two patients, respectively, and were cultured in the presence or absence of MTA discs and/or CaCl(2) in order to investigate calcium release, calcification activity, calcium-sensing receptor (CaSR) gene expression and bone morphogenetic protein-2 (BMP-2), and BMP-2 receptor protein and gene expression. RESULTS: MTA released a substantial accumulation of calcium (4 mmol/L) within 14 days into culture media. After 4 weeks, the two populations of HPLCs independently exhibited calcification as well as BMP-2 distribution in the vicinity of MTA. HPLCs inherently expressed genes encoding for the CaSR and BMP-2 receptors. Exogenous CaCl(2) media supplementation induced CaSR gene expression in HPLCs and calcification and BMP-2 synthesis throughout the entire HPLC cultures, whereas MgCl(2) had no effect. Both MTA and CaCl(2) stimulated BMP-2 gene expression above that of baseline levels. CONCLUSION: Here we show the first report showing that HPLCs cocultured directly with MTA up-regulated BMP2 expression and calcification. These results may be through CaSR interactions that were potentially activated by the release of calcium from MTA into the culture environment.

    DOI: 10.1016/j.joen.2009.12.024

  • Mineral trioxide aggregate induces bone morphogenetic protein-2 expression and calcification in human periodontal ligament cells Reviewed International journal

    Maeda H, Nakano T, Tomokiyo A, Fujii S, Wada N, Monnouchi S, Hori K, Akamine A

    J Endod   36 ( 4 )   2010.4

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  • MTAがヒト歯根膜線維芽細胞に及ぼす影響に関する研究 Reviewed

    前田英史,友清淳,藤井慎介、島一也,和田尚久,門野内聡,堀清美,中野嗣久,吉嶺嘉人,赤峰昭文

    日本歯科保存学会雑誌   52 ( 4 )   2009.6

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  • Immunomodulatory properties of human periodontal ligament stem cells. Reviewed International journal

    Naohisa Wada, Danijela Menicanin, Songtao Shi, P Mark Bartold, Stan Gronthos

    Journal of cellular physiology   219 ( 3 )   667 - 76   2009.6

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    Tissue engineering utilizing periodontal ligament stem cells (PDLSCs) has recently been proposed for the development of new periodontal regenerative therapies. Although the use of autologous PDLSC transplantation eliminates the potential of a significant host immune response against the donor cells, it is often difficult to generate enough PDLSCs from one donor source due to the variation of stem cell potential between donors and disease state of each patient. In this study, we examined the immunomodulatory properties of PDLSCs as candidates for new allogeneic stem cell-based therapies. Human PDLSCs displayed cell surface marker characteristics and differentiation potential similar to bone marrow stromal stem cells (BMSSCs) and dental pulp stem cells (DPSCs). PDLSCs, BMSSCs, and DPSCs inhibited peripheral blood mononuclear cell (PBMNC) proliferation stimulated with mitogen or in an allogeneic mixed lymphocyte reaction (MLR). Interestingly, gingival fibroblasts (GFs) also suppressed allogeneic PBMNC proliferation under both assay conditions. PDLSCs, BMSSCs, DPSCs, and GFs exhibited non-cell contact dependent suppression of PBMNC proliferation in co-cultures using transwells. Furthermore, conditioned media (CM) derived from each cell type pretreated with IFN-gamma partially suppressed PBMNC proliferation when compared to CMs without IFN-gamma stimulation. In all of these mesenchymal cell types cultured with activated PBMNCs, the expression of TGF-beta1, hepatocyte growth factor (HGF) and indoleamine 2, 3-dioxygenase (IDO) was upregulated while IDO expression was upregulated following stimulation with IFN-gamma. These results suggest that PDLSCs, BMSSCs, DPSCs, and GFs possess immunosuppressive properties mediated, in part, by soluble factors, produced by activated PBMNCs. J. Cell. Physiol. 219: 667-676, 2009. (c) 2009 Wiley-Liss, Inc.

    DOI: 10.1002/jcp.21710

  • Investigating a clonal human periodontal ligament progenitor/stem cell line in vitro and in vivo. Reviewed International journal

    Shinsuke Fujii, Hidefumi Maeda, Naohisa Wada, Atsushi Tomokiyo, Masahiro Saito, Akifumi Akamine

    Journal of cellular physiology   215 ( 3 )   743 - 9   2008.6

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    The lifespan of the tooth is influenced by the periodontal ligament (PDL), a specialized connective tissue that connects the cementum with the tooth socket bone. Generation of a cell line from PDL progenitor/stem cells would allow development of tissue engineering-based regenerative PDL therapy. However, little is known about the characteristics of PDL progenitor/stem cells because PDL tissue consists of a heterogeneous cell population and there are no pure PDL cell lines. Recently, we succeeded in immortalizing primary human PDL fibroblasts (HPLFs) by transfecting them with SV40 T-antigen and hTERT (Cell Tissue Res 2006; 324: 117-125). In this study, we isolated three clonal cell lines from these immortalized cells (lines 1-4, 1-11, and 1-24) that express RUNX-2, Col I, ALP, OPN, OCN, RANKL, OPG, scleraxis, periostin, Col XII, and alpha-SMA mRNA. Immunocytochemical analysis demonstrated that CD146 was expressed in cell lines 1-4 and 1-11 and that STRO-1 was expressed in lines 1-11 and 1-24. Lines 1-4 and 1-11 differentiated into osteoblastic cells and adipocytes when cultured in lineage-specific differentiation media. Four weeks after transplanting cell line 1-11 into immunodeficient mice with beta-tricalcium phosphate (beta-TCP), the transplant produced cementum/bone-like tissues around the beta-TCP. Eight weeks after transplantation, the 1-11 cell transplant formed PDL-like structures on the surface of the beta-TCP. These data suggest that cell line 1-11 was derived from a progenitor/stem cell present in the PDL and should be very useful for studying the biology and regeneration of human periodontium.

    DOI: 10.1002/jcp.21359

  • Development of a multipotent clonal human periodontal ligament cell line. Reviewed International journal

    Atsushi Tomokiyo, Hidefumi Maeda, Shinsuke Fujii, Naohisa Wada, Kazuya Shima, Akifumi Akamine

    Differentiation; research in biological diversity   76 ( 4 )   337 - 47   2008.4

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    The periodontal ligament (PDL) that anchors the tooth root to the alveolar bone influences the lifespan of the tooth, and PDL lost through periodontitis is difficult to regenerate. The development of new PDL-regenerative therapies requires the isolation of PDL stem cells. However, their characteristics are unclear due to the absence of somatic PDL stem cell lines and because PDL is composed of heterogeneous cell populations. Recently, we succeeded in immortalizing human PDL fibroblasts that retained the properties of the primary cells. Therefore, we aimed to establish a human PDL-committed stem cell line and investigate the effects of basic fibroblast growth factor (bFGF) on the osteoblastic differentiation of the cells. Here, we report the development of cell line 1-17, a multipotent clonal human PDL cell line that expresses the embryonic stem cell-related pluripotency genes Oct3/4 and Nanog, as well as the PDL-related molecules periostin and scleraxis. Continuous treatment of cell line 1-17 with bFGF in osteoblastic induction medium inhibited its calcification, with down-regulated expression of FGF-Receptor 1 (FGF-R1), whereas later addition of bFGF potentiated its calcification. Furthermore, bFGF induced calcification of cell line 1-17 when it was co-cultured with osteoblastic cells. These results suggest that cell line 1-17 is a PDL-committed stem cell line and that bFGF exerts dualistic (i.e., promoting and inhibitory) effects on the osteoblastic differentiation of cell line 1-17 based on its differentiation stage.

    DOI: 10.1111/j.1432-0436.2007.00233.x

  • EDTAならびにNaOClによる根管洗浄後のSEM観察 -超音波洗浄との比較- Reviewed

    島一也、前田英史、後藤康治、畦森雅子、安田善之、和田尚久、藤井慎介、友清淳、吉嶺嘉人、齋藤隆史、赤峰昭文

    日本歯内療法学会雑誌   29 ( 1 )   2008.1

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    SEM images of root canal dentin irrigated with EDTA and NaOCl -Comparison with ultrasonic irrigation-

  • 九州大学病院歯科医師臨床研修における協力型施設と研修歯科医のマッチング Reviewed

    松家洋子、王丸寛美、住吉圭太、伊吹禎一、和田尚久、角義久、秋山陽一、樋口勝規

    日本歯科医学教育学会雑誌   2007.4

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  • 九州大学病院歯科医師臨床研修における卒直後教育環境評価 Reviewed

    王丸寛美、角 義久、伊吹禎一、松家洋子、住吉圭太、和田尚久、秋山陽一、樋口勝規

    日本歯科教育学会誌   23 ( 2 )   2007.2

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  • MTAおよびSuper-Bondのヒト歯根膜細胞の骨芽細胞様分化に及ぼす影響に関する研究 Reviewed

    野田亮、前田英史、藤井慎介、和田尚久、友清淳、吉嶺嘉人、赤峰昭文

    日本歯内療法学会雑誌   2006.9

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    Effects of root-end filling materials on the osteoblast-like differentiation of human periodontal ligament cells

  • Establishing and characterizing human periodontal ligament fibroblasts immortalized by SV40T-antigen and hTERT gene transfer. Reviewed International journal

    Shinsuke Fujii, Hidefumi Maeda, Naohisa Wada, Yoshio Kano, Akifumi Akamine

    Cell and tissue research   324 ( 1 )   117 - 25   2006.4

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    The periodontal ligament (PDL) is a highly specialized tissue connecting the cementum with the tooth socket bone and affects the life span of the tooth. However, little is known about the precise characteristics and regenerative mechanism of PDL cells because of the absence of specific markers and cell lines. Therefore, we aimed to establish three immortalized human PDL fibroblast cell lines by using simian virus40 T-antigen (SV40T-Ag) and human telomerase reverse transcriptase (hTERT) transfection, expecting these cells to have the characteristics of primary cells. The transfected cells were named STPLF. The expression of SV40T-Ag and hTERT in all STPLF lines was verified by using the semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) method, stretch PCR analysis, or Western blotting analysis. All STPLF showed stable proliferation at more than 120 population doublings (PD), whereas primary human PDL fibroblasts (HPLF) stopped at 10-20 PD. Characterization by RT-PCR analysis revealed that all STPLF genes mimicked the expression of their respective original HPLF genes. STPLF expressed runt-related transcription factor-2, osterix, alkaline phosphatase, osteopontin, osteocalcin, periostin, receptor activator of NF-kappa B ligand, osteoprotegerin, epidermal growth factor receptor, alpha-smooth muscle actin, and type XII collagen. STPLF stimulated with 50 micro g/ml ascorbic acid and 2 mM beta-glycerophosphate for 4 weeks produced more calcified deposits than did HPLF cultured with the same reagents. These results suggest that each STPLF line retained the characteristics of the respective original HPLF, that STPLF gained increased calcification activity, and that STPLF are helpful tools for studying the biology and regenerative mechanisms of human PDL.

    DOI: 10.1007/s00441-005-0101-4

  • Identification of genes differentially expressed in osteoclast-like cells. Reviewed International journal

    Hisayuki Nomiyama, Kimie Egami, Naohisa Wada, Kazuko Tou, Madoka Horiuchi, Hiromi Matsusaki, Retsu Miura, Osamu Yoshie, Toshio Kukita

    Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research   25 ( 4 )   227 - 31   2005.4

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    Homeostasis of the skeletal system is maintained by a balance between bone formation and resorption. The receptor activator of NF-kappaB ligand (RANKL) induces the differentiation of bone-resorbing cells, osteoclasts. To identify genes regulated during osteoclast differentiation, we constructed a subtraction cDNA library using a mouse RAW264 macrophage cell line that differentiates into osteoclast-like multinucleated cells after treatment with RANKL. Northern blot analysis showed that RANKL treatment upregulated expression of 17 genes. Among these were the genes for five H(+)-ATPase subunits, two chemokines, and the osteoclast marker cathepsin K. In addition, a mouse homolog of human dendritic cell (DC)-specific transmembrane protein (DCSTAMP), whose function in osteoclastogenesis was recently revealed, was also included in the induced genes. Characterization of these inducible genes will provide an insight into the biology of osteoclasts and the mechanism of bone-related diseases.

  • Fibroblastic cells from human periapical granulation tissue preferentially form calcified matrices in decalcified boiled rat bone. Reviewed International journal

    Hidefumi Maeda, Naohisa Wada, Shinsuke Fujii, Akifumi Akamine

    Cell and tissue research   320 ( 1 )   135 - 40   2005.4

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    We have been studying the potential of human fibroblastic cells (HFC) from periapical granulation tissue to form a calcified matrix. Recently, we reported that inflamed periapical granulation tissue contains osteogenic cells. In the present study, we tested the hypothesis that HFC, cultured with decalcified bone (DB) of rat, might form much greater calcified matrices than with rat decalcified boiled bone (DBB), which was originally prepared as a negative control. HFC were cultured with DB or DBB in the presence or absence of 2 mM beta-glycerophosphate (beta-GP) and 50 microg/ml ascorbic acid. After six weeks of culture, a number of von Kossa-positive globular structures were unexpectedly observed inside DBB, but not DB. Without HFC, such structures were never seen in DBB incubated with 2 mM beta-GP and 50 microg/ml ascorbic acid. DB cultured with HFC under the same conditions did not show these structures. Electron-microscopic observation revealed that matrix vesicles aggregated on collagen fibrils around globular structures in DBB. Energy dispersive X-ray microanalysis confirmed that these structures were calcified matrices composed of calcium and phosphate. These results suggest that human periapical granulation tissue contains cells responsible for the formation of calcified matrices in DBB, and that DBB could serve as an excellent scaffold for the calcification of HFC, rather than DB.

    DOI: 10.1007/s00441-004-1052-x

  • Fibroblastic cells from human periapical granulation tissue preferentially form calcified matrices in decalcified and boiled rat bone. Reviewed International journal

    Maeda H, Wada N, Fujii S, Akamine A

    Cell and Tissue Research   320 ( 1 )   135 - 140   2005.1

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    DOI: 10.1007/s00441-004-1052-x

  • RANKL-induced DC-STAMP is essential for osteoclastogenesis. Reviewed International journal

    Toshio Kukita, Naohisa Wada, Akiko Kukita, Takashi Kakimoto, Ferry Sandra, Kazuko Toh, Kengo Nagata, Tadahiko Iijima, Madoka Horiuchi, Hiromi Matsusaki, Kunio Hieshima, Osamu Yoshie, Hisayuki Nomiyama

    The Journal of experimental medicine   200 ( 7 )   941 - 6   2004.10

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    Osteoclasts are bone-resorbing, multinucleated giant cells that are essential for bone remodeling and are formed through cell fusion of mononuclear precursor cells. Although receptor activator of nuclear factor-kappaB ligand (RANKL) has been demonstrated to be an important osteoclastogenic cytokine, the cell surface molecules involved in osteoclastogenesis are mostly unknown. Here, we report that the seven-transmembrane receptor-like molecule, dendritic cell-specific transmembrane protein (DC-STAMP) is involved in osteoclastogenesis. Expression of DC-STAMP is rapidly induced in osteoclast precursor cells by RANKL and other osteoclastogenic stimulations. Targeted inhibition of DC-STAMP by small interfering RNAs and specific antibody markedly suppressed the formation of multinucleated osteoclast-like cells. Overexpression of DC-STAMP enhanced osteoclastogenesis in the presence of RANKL. Furthermore, DC-STAMP directly induced the expression of the osteoclast marker tartrate-resistant acid phosphatase. These data demonstrate for the first time that DC-STAMP has an essential role in osteoclastogenesis.

  • Lipopolysaccharide stimulates expression of osteoprotegerin and receptor activator of NF-kappa B ligand in periodontal ligament fibroblasts through the induction of interleukin-1 beta and tumor necrosis factor-alpha. Reviewed International journal

    Naohisa Wada, Hidefumi Maeda, Yoshito Yoshimine, Akifumi Akamine

    Bone   35 ( 3 )   629 - 35   2004.9

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    Our recent work showed that human periodontal ligament fibroblasts (HPLF) secrete bioactive osteoprotegerin (OPG), which inhibits osteoclastic differentiation and activity. However, it is unknown how HPLF regulate bone metabolism in the presence of lipopolysaccharide (LPS), which is a cell component of gram-negative bacteria and a pathogen in inflammatory bone diseases such as periodontitis. The present study examined the effects of Escherichia coli LPS on the gene expression of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), OPG, and receptor activator of NF-kappa B ligand (RANKL) in HPLF using semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis. In HPLF cultured with LPS for 48 h, expression of both OPG and RANKL mRNA was up-regulated, whereas for up to 24 h of stimulation, such up-regulation was not observed. However, LPS increased expression of IL-1 beta and TNF-alpha mRNA within 6 h of treatment. Moreover, in HPLF cultured with IL-1 beta or TNF-alpha, OPG and RANKL expression was induced within 12 h of culture. The administration of neutralizing antibodies against human IL-1 beta or TNF-alpha to LPS-treated cultures of HPLF inhibited the induction of OPG and RANKL expression. These suggest that LPS stimulates both OPG and RANKL expression in HPLF by up-regulating IL-1 beta and TNF-alpha. In addition, administration of conditioned medium (CM) from HPLF (HPLF-CM) stimulated with LPS for 48 h to mouse bone marrow culture failed to induce osteoclast-like cell (OCL) formation. When mouse spleen cells were cocultured with HPLF in the presence of LPS, OCL formation was completely blocked. Taken together, our results indicate that human periodontal ligament cells stimulated with LPS inhibit osteoclastogenesis by producing more effective OPG than RANKL via the induction of IL-1 beta and TNF-alpha.

    DOI: 10.1016/j.bone.2004.04.023

  • Human periapical granulation tissue contains osteogenic cells. Reviewed International journal

    Hidefumi Maeda, Naohisa Wada, Hiroyoshi Nakamuta, Akifumi Akamine

    Cell and tissue research   315 ( 2 )   203 - 8   2004.2

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    Bone defects caused by periapical inflammation can be treated and improved by endodontic therapy. However, the mechanism for osseous healing of periapical lesions after root canal treatment is unclear. In this study we examined whether fibroblastic cells from human periapical granulation tissue could produce calcified matrix in vitro. Periapical lesions from three patients were dissected in endodontic surgery, and fibroblastic cells (HFC) migrating from these lesions in vitro were used in this study. The HFC were cultured with or without beta-glycerophosphate (beta-GP) and ascorbic acid (AA), and the expression of human runt-related transcription factor-2 (Runx2), osterix (Osx), osteopontin (Opn), and osteocalcin (Ocn) mRNA, and alkaline phosphatase (ALPase) was examined by reverse transcriptase-polymerase chain reaction (RT-PCR) or by an enzyme-cytochemical technique. Furthermore, calcification in the cells was investigated by von Kossa staining. At the beginning of the culture, HFC expressed Runx2 mRNA faintly, but neither Opn mRNA nor ALPase activity. Immunocytochemical study also showed HFC expressed Runx2 more weakly, compared to SaOS2. However, the expression levels of ALPase, and Runx2, Osx, and Opn mRNA, were stimulated by 2 mM beta-GP and 50 microg/ml AA. After 4 weeks of culture with 2 mM beta-GP and 50 microg/ml AA, HFC formed von Kossa staining-positive calcified deposits on culture dishes, and also expressed Ocn mRNA. These results suggest that inflamed periapical granulation tissue contains osteogenic cells that have the potential to differentiate into mature osteoblastic or cementoblastic cells, and that such cells might contribute to osseous healing after root canal treatment.

    DOI: 10.1007/s00441-003-0832-z

  • Direct stimulation of osteoclastogenesis by MIP-1: evidence obtained from studies using RAW 264 cell clone highly responsive to RANKL. Reviewed International journal

    Watanabe T, Kukita T, Kukita A, Wada N, Toh K, Nagata K, Nomiyama H, Iijima T

    Journal of Endocrinology   180 ( 1 )   193 - 201   2004.1

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    DOI: 10.1677/joe.0.1800193

  • Direct stimulation of osteoclastogenesis by MIP-1alpha: evidence obtained from studies using RAW264 cell clone highly responsive to RANKL. Reviewed International journal

    Toshiyuki Watanabe, Toshio Kukita, Akiko Kukita, Naohisa Wada, Kazuko Toh, Kengo Nagata, Hisayuki Nomiyama, Tadahiko Iijima

    The Journal of endocrinology   180 ( 1 )   193 - 201   2004.1

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    Macrophage inflammatory protein-1alpha (MIP-1alpha) is a member of the CC chemokines. We have previously reported the use of a whole bone marrow culture system to show that MIP-1alpha stimulates the formation of osteoclast-like multinucleated cells. Here we use rat bone marrow cells deprived of stromal cells, and clones obtained from murine macrophage-like cell line RAW264 to show that MIP-1alpha acts directly on cells in osteoclast lineage. We obtained several types of RAW264 cell clones, one of these clones, designated as RAW264 cell D clone (D clone), showed an extremely high response to receptor activator of NFkappaB ligand (RANKL) and tumor necrosis factor-alpha (TNF-alpha), while the other clone, RAW264 cell N clone (N clone), demonstrated no response to RANKL or TNF-alpha. Although both clones expressed receptor activator NFkappaB (RANK) before being stimulated for differentiation, only the D clone expressed cathepsin K when cells were stimulated to differentiate to osteoclasts. MIP-1alpha stimulated the formation of mononuclear preosteoclast-like cells from rat bone marrow cells deprived of stromal cells. MIP-1alpha also stimulated formation of osteoclast-like multinucleated cells from the D clone, when these cells were stimulated with RANKL and TNF-alpha. These findings provide strong evidence to show that MIP-1alpha acts directly on cells in the osteoclast lineage to stimulate osteoclastogenesis. Furthermore, pretreatment of RAW264 cell D clone with MIP-1alpha significantly induced adhesion properties of these cells to primary osteoblasts, suggesting a crucial role for MIP-1alpha in the regulation of the interaction between osteoclast precursors and osteoblasts in osteoclastogenesis.

  • Human periapical granulation tissue contains osteogenic cells. Reviewed International journal

    Maeda H, Wada N, Nakamuta H, Akamine A

    Cell and Tissue Research   315 ( 2 )   203 - 208   2004.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00441-003-0832-z

  • An epidemiologic examination on the prevalence of the periodontal diseases and oral pigmentation in Yusho patients in 2002. Reviewed

    Isamu Hashiguchi, Yoshito Yoshimine, Yasuharu Gotou, Hidefumi Maeda, Naohisa Wada, Akifumi Akamine, Hiroshi Fukuyama, Hidehiko Okumura

    Fukuoka igaku zasshi = Hukuoka acta medica   94 ( 5 )   81 - 6   2003.5

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    An epidemiologic examination was carried out to reveal the prevalence of the periodontal diseases and oral pigmentation in patients with Yusho. The results obtained were as follows. 1) 95 patients out of 110 patients, who were examined periodontal pocket depth using Ramfjord' methods, had at least one tooth with periodontal pocket deeper than 3 mm. Similarly, 276 teeth out of a total 495 examined teeth showed periodontal pockets with more than 3 mm depth. However, the ratio of the teeth with periodontal pockets deeper than 4 mm to total examined teeth in each age fell to less than 25%. 2) Oral pigmentation was observed in 75 patients out of 121 patients with Yusho. In this examination, gingival pigmentation was most predominant among oral pigmentation. It is of particular interest that severe pigmentation tended to be observed at a much higher frequency in younger patients with Yusho. Taken these findings into consideration, it was suggested that PCBs and related compounds might play an important role in the development of both periodontal diseases and oral pigmentation.

    DOI: 10.15017/18728

  • An epidemiologic examination on the prevalence of the periodontal diseases and oral pigmentation in yusho patients in 2002. . 94: 81-86, 2003. Reviewed

    Hashiguchi I, Yoshimine Y, Gotou Y, Maeda H, Wada N, Akamine A, Fukuyama H, Okumura H

    Fukuoka Acta Medica   2003.1

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  • Histological study of periapical tissue healing in the Rat Molar after retrofilling with various materials. Reviewed International journal

    Maeda H, Hashiguchi I, Nakamuta H, Toriya Y, Wada N, Akamine A

    Journal of Endodontics   25 ( 1 )   38 - 42   1999.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0099-2399(99)80397-5

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Books

  • Dental Stem Cells

    Naohisa Wada, Atsushi Tomokiyo, Hidefumi Maeda(Role:Joint author)

    Springer International Publishing Switzerland  2016.5 

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    Language:English   Book type:Scholarly book

  • 私の“Single Patient Use”&“シングルファイル”の臨床応用 感染対策の視点で In: 新しいNi-Ti製ファイルの歯内療法-Single Patient Use時代の到来-

    和田 尚久, 赤峰 昭文(Role:Joint author)

    クインテッセンス出版、東京  2014.9 

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    Responsible for pages:2章-2, 41-47   Language:Japanese   Book type:General book, introductory book for general audience

  • Periodontal ligament stem cells. In: Stem Cells in Clinic and Research

    Hidefumi Maeda, Naohisa Wada, Fujii Shinsuke, Atsushi Tomokiyo, Akifumi Akamine(Role:Joint author)

    InTech, Rijeka, Croatia  2011.7 

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    Responsible for pages:Chapter 25, 619-636   Language:English   Book type:Scholarly book

  • 口腔乾燥症(ドライマウス) In: 今日の治療指針2024年版 Vol.66 ‐私はこう治療している‐

    和田尚久(Role:Joint author)

    医学書院  2024.1 

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    Language:Japanese   Book type:Scholarly book

  • 歯内療法学専門用語集 第2版

    日本歯科保存学会学術用語専門委員会

    2023.3 

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    Language:Japanese  

  • 保存修復学専門用語集 第3版

    日本歯科保存学会学術用語専門委員会

    2023.3 

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    Language:Japanese  

  • よだれが多い方の口腔ケア In: 介護福祉士のための口腔ケアマニュアル

    高木信恵、有水智香、和田尚久(Role:Joint author)

    2022.8 

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    Language:Japanese   Book type:Scholarly book

  • 侵襲的歯科処置時の偶発症 露髄・根管穿孔、髄床底穿孔、疼痛 急性歯髄炎/急性根尖性歯周炎/歯周病急発 In: 歯科診療・口腔ケアにおける救急&アクシデント対応ハンドブック

    和田尚久(Role:Joint author)

    医歯薬出版株式会社  2022.1 

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  • 口腔金属アレルギー In: 今日の治療指針2021年版 Vol.63 ‐私はこう治療している‐

    和田尚久(Role:Joint author)

    医学書院  2021.1 

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    Language:Japanese   Book type:Scholarly book

  • よき歯科医療人になるための倫理・プロフェッショナリズム教育 プロフェッションワークブック

    木尾哲郎、浅沼直樹、尾﨑哲則、樫則章、角忠輝、長谷由紀子、平田創一郎、星野由美、山本龍生、和田尚久(Role:Joint author)

    医歯薬出版  2019.9 

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    Language:Japanese   Book type:Scholarly book

  • Frontiers in stem cell and regeneration medicine research vol.2

    Atsushi Tomokiyo, Naohisa Wada, Hidefumi Maeda(Role:Joint author)

    Bentham Science Publishers  2016.2 

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    Responsible for pages:pp3-38   Language:English   Book type:Scholarly book

  • Differentiation of Periodontal Ligament Stem/Progenitor Cells: Roles of TGF-β1. In: Stem Cells and Cancer Stem Cells, volume 4, Therapeutic Applications in Disease and Injury.

    Hidefumi Maeda, Fujii Shinsuke, Monnouchi Satoshi, naohisa wada, Akifumi Akamine(Role:Joint author)

    Springer, Heidelberg, Germany  2012.5 

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    Responsible for pages:Chapter 5, 51-58   Language:English   Book type:Scholarly book

  • Induction of BMP-2 in periodontal ligament cells by calcium-based biomaterial. In: Bone Morphogenetic Proteins: New Research

    Hidefumi Maeda, naohisa wada, Atsushi Tomokiyo, Akifumi Akamine(Role:Joint author)

    Nova Science Publishers, New York  2012.4 

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    Responsible for pages:Chapter 10, 187-202   Language:English   Book type:General book, introductory book for general audience

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Presentations

  • 歯科用マイクロスコープを用いたエンド治療 Invited

    和田尚久

    第33回日本歯科人間工学会研究発表大会  2017.12 

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    Event date: 2017.12

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:九州大学病院キャンパスコラボステーションⅠ(福岡市)   Country:Japan  

  • 総合歯科医に求められる役割を考える-口腔総合診療科に所属する一歯科保存専門医の立場から- Invited

    和田尚久

    第10回日本総合歯科学会総会・学術大会  2017.11 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:アートホテル新潟駅前,新潟大学 駅南キャンパス ときめいと(新潟市)   Country:Japan  

  • Transgelin Mediates TGF-beta1-induced Human Periodontal Ligament Cell Proliferation. International conference

    Hiromi Mitarai, Naohisa Wada, Daigaku Hasegawa, Shinichiro Yoshida, Mai Sonoda, Atsushi Tomokiyo, Sayuri Hamano, Hidefumi Maeda

    95th General Session & Exhibition of the International Association for Dental Research (IADR)  2017.3 

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    Event date: 2017.3

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Venue:San Francisco, Calif.   Country:United States  

  • Directing human iPS cells toward PDL stem cells International conference

    Sayuri Hamano, Atsushi Tomokiyo, Naohisa Wada, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Suguru Serita, Hiroyuki Mizumachi, Hiromi Mitarai, Hidefumi Maeda

    94th General Session & Exhibition of the International Association for Dental Research (IADR)  2016.6 

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    Event date: 2016.6

    Language:English  

    Venue:CoEx Convention & Exhibition Center, Seoul   Country:United States  

  • Semaphorin 3A induces odontoblastic phenotype in dental pulp stem cells. International conference

    Shinichiro Yoshida, Naohisa Wada, Daigaku Hasegawa, Atsushi Tomokiyo, Sayuri Hamano, Hiromi Mitarai, Hideki Sugii, Hidefumi Maeda

    94th General Session & Exhibition of the International Association for Dental Research (IADR)  2016.6 

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    Event date: 2016.6

    Language:English  

    Venue:CoEx Convention & Exhibition Center, Seoul   Country:United States  

  • Identification of a novel periodontal ligament stem cell marker International conference

    Daigaku Hasegawa, Naohisa Wada, Sayuri Hamano, Atsushi Tomokiyo, Shinichiro Yoshida, Hiromi Mitarai, Mai Sonoda, Hideki Sugii, Hidefumi Maeda

    94th General Session & Exhibition of the International Association for Dental Research (IADR)  2016.6 

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    Event date: 2016.6

    Language:English  

    Venue:CoEx Convention & Exhibition Center, Seoul   Country:United States  

  • 歯髄細胞におけるβig-h3の発現および機能について

    芹田 俊, 友清 淳, 長谷川 大学, 濱野 さゆり, 杉井英樹, 吉田 晋一郎, 山本直秀, 水町 博之, 御手洗 裕美, 和田 尚久, 前田 英史

    第144回日本歯科保存学会春季学術大会  2016.6 

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    Event date: 2016.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:栃木県総合分化センター(宇都宮市)   Country:Japan  

  • iPS細胞由来の歯根膜幹細胞様細胞の樹立

    濱野 さゆり, 友清 淳, 和田 尚久, 長谷川 大学, 杉井英樹, 吉田 晋一郎, 芹田 俊, 水町 博之, 御手洗 裕美, 前田 英史

    第144回日本歯科保存学会春季学術大会  2016.6 

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    Event date: 2016.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:栃木県総合分化センター(宇都宮市)   Country:Japan  

  • MESTはヒト歯根膜幹細胞における幹細胞特性の維持に関与する

    長谷川 大学, 和田 尚久, 濱野 さゆり, 友清 淳, 吉田 晋一郎, 御手洗 裕美, 園田 麻衣, 杉井英樹, 前田 英史

    第144回日本歯科保存学会春季学術大会  2016.6 

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    Event date: 2016.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:栃木県総合分化センター(宇都宮市)   Country:Japan  

  • Semaphorin 3A induces odontoblastic phenotype in dental pulp stem cells. International conference

    Shinichiro Yoshida, Naohisa Wada, Daigaku Hasegawa, Atsushi Tomokiyo, Sayuri Hamano, Hiromi Mitarai, Serita S, Mizumachi H, Hidefumi Maeda

    Kyudai Oral Bioscience 2016 -Frontiers in Dental Research and Education in East Asia-  2016.2 

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    Event date: 2016.2

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Fukuoka   Country:Japan  

  • 炎症性サイトカインで刺激したヒト歯根膜細胞由来のGDNFはPC12の神経細胞分化を促進する

    吉田 晋一郎, 山本直秀, 和田 尚久, 友清 淳, 長谷川 大学, 濱野 さゆり, 祐田 明香, 御手洗 裕美, 杉井英樹, 前田 英史

    第143回日本歯科保存学会秋季学術大会  2015.11 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:文京シビックホール(東京都)   Country:Japan  

  • 歯根膜細胞におけるα-SMA発現にTransgelinが関与する

    御手洗 裕美, 和田 尚久, 前田 英史, 長谷川 大学, 吉田 晋一郎, 濱野 さゆり, 祐田 明香, 友清 淳, 赤峰 昭文

    第142回日本歯科保存学会春季学術大会  2015.6 

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    Event date: 2015.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場(北九州市)   Country:Japan  

  • Semaphorin3Aがヒト歯髄幹細胞による硬組織形成に及ぼす影響

    吉田 晋一朗, 和田 尚久, 前田 英史, 門野内 聡, 長谷川 大学, 御手洗 裕美, 濱野さゆり, 祐田明香, 杉井英樹, 赤峰 昭文

    第140回日本歯科保存学会春季学術大会  2014.6 

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    Event date: 2014.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:滋賀県立芸術劇場(大津市)   Country:Japan  

  • Wnt5aはTGFβ1を介してヒト歯根膜細胞のコラーゲン線維形成を促進する

    長谷川 大学, 和田 尚久, 前田 英史, 吉田 晋一朗, 門野内 聡, 御手洗 裕美, 濱野さゆり, 祐田明香, 赤峰 昭文

    第140回日本歯科保存学会春季学術大会  2014.6 

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    Event date: 2014.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:滋賀県立芸術劇場(大津市)   Country:Japan  

  • Wnt5aはRor2-JNKシグナルを介してヒト歯根膜幹細胞株の骨芽細胞様分化を抑制する

    長谷川大学, 和田 尚久, 前田 英史, 吉田晋一朗, 御手洗裕美, 門野内 聡, 濱野さゆり, 祐田明香, 赤峰 昭文

    第141回日本歯科保存学会秋季学術大会  2014.6 

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    Event date: 2014.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:山形テルサ(山形市)   Country:Japan  

  • The Effects of Wnt5a on Human Periodontal Ligament Cells. International conference

    Daigaku Hasegawa, Naohisa Wada, Hidefumi Maeda, Shinichiro Yoshida, Monnouchi Satoshi, Koori Katsuaki, Sayuri Hamano, Hiromi Mitarai, Akifumi Akamine

    Kyudai Oral Bioscience 2014 - 8th International Symposium -  2014.2 

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    Event date: 2014.2 - 2014.3

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Fukuoka Recent Hotel, Fukuoka   Country:Japan  

  • Dental pulp stem cells on bone tissue express periodontal ligament-related gene. International conference

    Shinichiro Yoshida, naohisa wada, Hidefumi Maeda, Daigaku Hasegawa, Sato H, Monnouchi Satoshi, Akifumi Akamine

    The 9th World Endodontic Congress  2013.5 

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    Event date: 2013.5

    Language:English  

    Venue:Tokyo International Forum, Tokyo   Country:Japan  

  • Conversion of periodontal ligament cells into multipotent stem-like cells International conference

    naohisa wada, Hidefumi Maeda, Daigaku Hasegawa, Stan Gronthos, P.Mark Bartold, Daniela Menicanin, Fujii Shinsuke, Hiroko Wada, Atsushi Tomokiyo, Monnouchi Satoshi, Akifumi Akamine

    91st General Session & Exhibition of the IADR  2013.3 

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    Event date: 2013.3

    Language:English  

    Venue:Seattle   Country:United States  

  • 再生医療における歯原性幹細胞の可能性 Invited

    和田 尚久

    日本再生歯科医学会2013シンポジウム「よみがえる歯髄・歯根膜・根尖歯周組織」  2013.2 

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    Event date: 2013.2

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:西日本総合展示場AIMビル(北九州市)   Country:Japan  

  • Induction of stem/undifferentiated cells from human periodontal ligament cells by Sema3A International conference

    naohisa wada, Hidefumi Maeda, Daigaku Hasegawa, Stan Gronthos, P.Mark Bartold, Daniela Menicanin, Fujii Shinsuke, Hiroko Wada, Atsushi Tomokiyo, Monnouchi Satoshi, Akifumi Akamine

    The Australian Health and Medical Research Congress 2012  2012.11 

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    Event date: 2012.11

    Language:English  

    Venue:Adelaide Convention Centre, Adelaide   Country:Australia  

  • 分化能の異なるヒト歯根膜細胞クローンの単離及びキャラクタライゼーション

    長谷川大学, 和田 尚久, 前田 英史, 藤井 慎介, 郡勝明, 友清 淳, 門野内 聡, 河野 清美, 山本直秀, 寺松陽子, 濱野さゆり, 祐田明香, 赤峰 昭文

    第137回日本歯科保存学会秋季学術大会  2012.11 

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    Event date: 2012.11

    Language:Japanese  

    Venue:広島国際会議場(広島市)   Country:Japan  

  • Sema3Aがヒト歯根膜細胞の幹細胞/未分化細胞誘導に及ぼす影響

    和田 尚久, 前田 英史, 長谷川 大学, 藤井 慎介, 山本 直秀, 門野内 聡, 赤峰 昭文

    136回日本歯科保存学会春季学術大会  2012.6 

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    Event date: 2012.6

    Language:Japanese  

    Venue:沖縄コンベンションセンター(宜野湾市)   Country:Japan  

  • Immunosuppressive properties of human periodontal ligament stem cells International conference

    Wada N, Gronthos S, Menicanin D, Mrozik K, and Bartold PM

    87th General Session & Exhibition of the IADR  2009.4 

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    Event date: 2009.4

    Venue:Miami   Country:United States  

    Immunosuppressive properties of human periodontal ligament stem cells

  • Development of a multipotent clonal human periodontal ligament cell line International conference

    Tomokiyo A, Maeda H, Fujii S, Wada N, Shima K, Akamine A

    Dental and Craniofacial morphogenesis and tissue regeneration  2007.3 

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    Presentation type:Symposium, workshop panel (public)  

    Venue:Fukuoka   Country:Japan  

  • ヒト不死化歯根膜クローン細胞におけるEMDおよびbFGFが石灰化誘導に及ぼす影響

    友清淳、前田英史、藤井慎介、和田尚久、赤峰昭文

    第123回日本歯科保存学会秋季大会  2005.11 

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    Presentation type:Symposium, workshop panel (public)  

    Venue:東京都   Country:Japan  

  • Characterization of clonal human periodontal ligament cell lines. International conference

    Fujii S, Maeda H, Wada N, Tomokiyo A, Akamine A

    Dental and Craniofacial morphogenesis and tissue regeneration  2006.3 

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    Presentation type:Oral presentation (general)  

    Venue:Fukuoka   Country:Japan  

  • Effects of bFGF and EMD on human periodontal ligament cells. International conference

    Tomokiyo A, Maeda H, Fujii S, Wada N, Akamine A

    84th General Session & Exhibition of the IADR  2006.6 

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    Presentation type:Symposium, workshop panel (public)  

    Venue:Brisbane   Country:Australia  

  • Characterization of clonal human periodontal ligament cell lines. International conference

    Fujii S, Maeda H, Wada N, Tomokiyo A, Akamine A

    84th General Session & Exhibition of the IADR  2006.6 

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    Presentation type:Symposium, workshop panel (public)  

    Venue:Brisbane   Country:Australia  

  • 多分化能を持つヒト歯根膜クローン細胞株の樹立とキャラクタリゼーション

    友清淳、前田英史、藤井慎介、和田尚久、島一也、赤峰昭文

    第125回日本歯科保存学会秋季大会  2006.11 

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    Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島市   Country:Japan  

  • Investigating a clonal human periodontal ligament progenitor/stem cell line in vitro and in vivo. International conference

    Fujii S, Maeda H, Wada N, Tomokiyo A, Akamine A

    Dental and Craniofacial morphogenesis and tissue regeneration  2007.3 

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    Presentation type:Oral presentation (general)  

    Venue:Fukuoka   Country:Japan  

  • IGFBP3は歯胚発生と歯周組織のリモデリングに関与する

    #王恕心、@御手洗裕美、#冉子晴、@祐田明香、#孫偉浩、@原口晃、@前田英史、@和田尚久

    日本歯科保存学会  2023.11 

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    Event date: 2023.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:アクトシティ浜松(浜松市)   Country:Japan  

  • 著しい骨隆起を有する患者に対し包括的治療を行った症例

    @渡邉護煕,@御手洗裕美,@王丸寛美,@和田尚久

    日本総合歯科学会  2023.10 

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    Event date: 2023.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:日本大学歯学部本館創設百周年記念講堂(東京都)   Country:Japan  

  • 有床義歯の製作における補綴前処置について学んだ1症例

    @岡崎裕紀,@伊吹禎一,@和田尚久

    日本総合歯科学会  2023.10 

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    Event date: 2023.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:日本大学歯学部本館創設百周年記念講堂(東京都)   Country:Japan  

  • スーパーMTAペーストはヒト前骨芽細胞の石灰化誘導能を促進する

    @御手洗裕美、@Naati Fakatava、#王恕心、#冉子晴、@祐田明香、@原口晃、#孫偉浩、@和田尚久

    日本歯科保存学会  2023.6 

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    Event date: 2023.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:くにびきメッセ(松江市)   Country:Japan  

  • 有床義歯に強い抵抗感のある患者に対し義歯再製作を試みた1症例

    @久保健太郎,@伊吹禎一,@和田尚久

    日本総合歯科学会  2022.11 

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    Event date: 2022.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:九州歯科大学講堂ホール(北九州市)   Country:Japan  

  • 九州大学歯学部における診療参加型臨床実習に関する臨床実習生の意識調査

    @伊吹禎一、@寳田貫、@王丸寛美、@原口晃、@築山能大、@和田尚久

    日本歯科医学教育学会  2022.7 

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    Event date: 2022.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • ACTA2 regulates human PDL function via interaction with or without TGF-β1

    #Naati Fakatava、@御手洗裕美、@祐田明香、@原口晃、@長谷川大学、@前田英史、@和田尚久

    日本歯科保存学会  2022.6 

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    Event date: 2022.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • 全国の歯科患者における口腔の健康状態と身体的愁訴との縦断的な関連 ―8020推進財団 歯科医療による健康増進効果に関する研究―

    井上昂也、古田美智子、須磨紫乃、深井穫博、嶋崎義浩、相田潤、安藤雄一、宮崎秀夫、神原正樹、和田尚久、山下喜久

    第43回九州口腔衛生学会総会  2021.12 

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    Event date: 2021.12

    Language:Japanese  

    Venue:WEB   Country:Japan  

  • Relation between oral hypofunction and nutrient intake condition in the elderly International conference

    Hirata K, Ohmaru T, Wada N

    5th KOB-OBT Joint Symposium  2021.11 

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    Event date: 2021.11

    Language:Japanese  

    Venue:Fukuoka   Country:Japan  

  • 研修環境の評価に対する新型コロナウィルスの影響と研修形態の比較

    王丸寛美、伊吹禎一、寳田貫、原口晃、和田尚久

    第40回日本歯科医学教育学会総会および学術大会  2021.11 

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    Event date: 2021.11 - 2021.12

    Language:Japanese  

    Venue:オンライン   Country:Japan  

  • オンライン指導歯科医講習会の開催方法に関する研究

    長島正、田口則宏、井上哲、則武加奈子、長谷川篤司、和田尚久、野崎剛徳

    第40回日本歯科医学教育学会総会および学術大会  2021.11 

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    Event date: 2021.11 - 2021.12

    Language:Japanese  

    Venue:オンライン   Country:Japan  

  • 倫理・プロフェッショナリズム教育資源の活用状況に関する調査

    平田創一郎、木尾哲朗、鶴田潤、長谷由紀子、和田尚久

    第40回日本歯科医学教育学会総会および学術大会  2021.11 

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    Event date: 2021.11 - 2021.12

    Language:Japanese  

    Venue:オンライン   Country:Japan  

  • 我が国における社会系歯学教育についての調査研究

    平田創一郎、木尾哲朗、鶴田潤、長谷由紀子、和田尚久

    第40回日本歯科医学教育学会総会および学術大会  2021.11 

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    Event date: 2021.11 - 2021.12

    Language:Japanese  

    Venue:オンライン   Country:Japan  

  • 咬合崩壊した臼歯部の補綴治療を通し、咬合について学んだ1 症例

    与那嶺亮、茂田彩花、伊吹禎一、和田尚久

    第14回日本総合歯科学会総会・学術大会  2021.10 

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    Event date: 2021.10 - 2021.11

    Language:Japanese  

    Venue:オンライン   Country:Japan  

  • 患者とのラポール形成で抜歯即時義歯を製作できた1例

    信太実有、御手洗裕美、和田尚久

    第14回日本総合歯科学会総会・学術大会  2021.10 

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    Event date: 2021.10 - 2021.11

    Language:Japanese  

    Venue:オンライン   Country:Japan  

  • Terpinen-4-ol の根管内細菌に対する抗菌性の検討

    神谷治伸、原口晃、御手洗裕美、Fakatava Naati、祐田明香、前田英史、和田尚久

    日本歯科保存学会 2021年度秋季学術大会(第155回)  2021.10 

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    Event date: 2021.10 - 2021.11

    Language:Japanese  

    Venue:オンライン   Country:Japan  

  • 脳死肝移植により救命を得た患者の周術期等口腔機能管理に難渋した一症例

    有水智香、神野哲平、赤星朋比古、進藤幸之助、高嶋美甫、永田千尋、中村次代、稲井裕子、和田尚久

    第64回秋季日本歯周病学会学術大会  2021.10 

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    Event date: 2021.10

    Language:Japanese  

    Venue:オンライン   Country:Japan  

  • ビスホスホネート製剤由来味覚障害の分子機構の解明

    尾池麻未、岩田周介、平山彩夏、菅原友佳、大野友里花、川端由子、高井信吾、實松敬介、和田尚久、重村憲徳

    第55回大会日本味と匂学会  2021.9 

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    Event date: 2021.9

    Language:Japanese  

    Venue:九州大学医学部百年講堂(福岡市)   Country:Japan  

  • 広範熱傷患者への歯科的介入の検討

    木附智子、石井広太郎、神野哲平、平野奈々美、大山順子、赤星朋比古、和田尚久、森悦秀

    第18回日本口腔ケア学会総会・学術大会  2021.4 

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    Event date: 2021.4

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:伊藤国際学術研究センター他(東京都)   Country:Japan  

  • 九州大学病院救命 ICUにおける多職種連携に有効な歯科的情報共有方法の検討

    #有水智香、神野哲平、野口英里、桑田睦子、赤星朋比古、柏﨑晴彦、和田尚久

    第18回日本口腔ケア学会総会・学術大会  2021.4 

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    Event date: 2021.4

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:伊藤国際学術研究センター他(東京都)   Country:Japan  

  • 九州大学病院における高次脳機能障害患者に対して歯科衛生士が早期介入した一症例

    髙橋綾華、山添淳一、有水智香、高木信恵、柏﨑晴彦、和田尚久

    第18回日本口腔ケア学会総会・学術大会  2021.4 

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    Event date: 2021.4

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:伊藤国際学術研究センター他(東京都)   Country:Japan  

  • 九州大学病院周術口腔機能管理期患者において術後に起こり得る口腔内の病的所見の把握

    小林真由香、有水智香、今泉典子、平野菜々美、津田美穂、高橋綾華、浦邊薫、高木信恵、稲井裕子、柏﨑晴彦、和田尚久

    第18回日本口腔ケア学会総会・学術大会  2021.4 

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    Event date: 2021.4

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:伊藤国際学術研究センター他(東京都)   Country:Japan  

  • 血清LBP値のメタボリックシンドローム発症との関連:久山町研究

    #友岡祥子、大石絵美、津田雅子、秦淳、和田尚久、二宮利治

    第56回日本循環器病予防学会学術集会  2020.12 

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    Event date: 2020.12

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:オンライン   Country:Japan  

  • Roles of PDLIM5 in periodontal ligament and gingival epithelial cells. International conference

    Yuda A, Fujii S, McCulloch CA, Usui M, Murakami S, Maeda H, Wada N

    American Academy of Periodontology (AAP) 106th Annual Meeting  2020.11 

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    Event date: 2020.11

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Venue:Virtual Meeting(Honolulu)   Country:United States  

  • 初診医療面接における患者の解釈モデル聴取の重要性を学んだ1症例

    藤井菜央、伊吹禎一、和田尚久

    第13回日本総合歯科学会学術大会  2020.11 

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    Event date: 2020.10 - 2020.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:WEB開催(福岡市)   Country:Japan  

  • 胸部食道癌術後,脳梗塞及び誤嚥性肺炎を発症した患者に対する口腔ケア支援チームの取り組み

    今泉典子、稲井裕子、#有水智香、寶田貫、柏崎晴彦、和田尚久

    第17回日本口腔ケア学会総会・学術大会  2020.9 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:長崎ブリックホール(長崎市)   Country:Japan  

  • 当院ICU においてOHAT-J を活用した口腔ケア支援システムの取り組み

    @平野菜々美、@神野哲平、@野口英里、@堀智恵、#有水智香、@岡留朝子、@山添淳一、@和田尚久

    第17回日本口腔ケア学会総会・学術大会  2020.9 

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    Event date: 2020.9

    Language:Japanese  

    Venue:長崎ブリックホール(長崎市)   Country:Japan  

  • 九州大学病院周術期口腔ケアセンターにおける術後往診に関する調査

    津田美穂、#有水智香、今泉典子、高橋綾華、久家雅美、平野菜々美、寶田貫、稲井裕子、柏崎晴彦、和田尚久

    第17回日本口腔ケア学会総会・学術大会  2020.9 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:長崎ブリックホール(長崎市)   Country:Japan  

  • TransgelinはIntegrinを介した細胞が基質への接着に関与する

    @御手洗裕美、@祐田明香、#Naati Fakatava、@長谷川大学、@前田英史、@和田尚久

    日本歯科保存学会2020年度春季学術大会(第152回)  2020.6 

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    Event date: 2020.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:神戸国際展示場(誌上開催)   Country:Japan  

  • The role of ACTA2 in periodontal ligament cell stimulated with TGF-β1

    #Naati Fakatava、@御手洗裕美、@祐田明香、@長谷川大学、@前田英史、@和田尚久

    日本歯科保存学会2020年度春季学術大会(第152回)  2020.6 

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    Event date: 2020.6

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Venue:神戸国際展示場(誌上開催)   Country:Japan  

  • 九州大学病院耳鼻咽喉科周術期患者の口腔衛生管理で経験した1症例

    山田和貴子、王丸寛美、祐田明香、和田尚久

    第12回日本総合歯科学会学術大会  2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北海道歯科医師会館(札幌市)   Country:Japan  

  • 即時義歯の製作について学んだ1症例

    吉田崇裕、伊吹禎一、和田尚久

    第12回日本総合歯科学会学術大会  2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北海道歯科医師会館(札幌市)   Country:Japan  

  • 2種類の旧義歯の問題点を考察し,新義歯の設計を行った1症例

    尾池麻未、伊吹禎一、和田尚久

    第12回日本総合歯科学会学術大会  2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北海道歯科医師会館(札幌市)   Country:Japan  

  • 新義歯作製により口腔機能の向上を目指した1症例

    掛村友起子、祐田明香、王丸寛美、和田尚久

    第12回日本総合歯科学会学術大会  2019.11 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北海道歯科医師会館(札幌市)   Country:Japan  

  • 周術期口腔機能管理患者のセルフケア状況とその関連要因

    有水智香,神野哲平,田上裕梨,北岡優衣,白仁協,倉田理沙,祐田明香,平野菜々美,山添淳一,柏崎晴彦,和田尚久

    第62回秋季日本歯周病学会学術大会  2019.10 

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    Event date: 2019.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:西日本総合展示場・北九州国際会議場(北九州市)   Country:Japan  

  • Wnt/β-カテニン-Sema3aシグナルは唾液腺の発生を制御する -課外授業での研究活動報告-

    藤本龍史、藤井慎介、和田尚久、清島保

    第38回日本歯科医学教育学会学術大会  2019.7 

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    Event date: 2019.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:パピヨン24(福岡市)   Country:Japan  

  • Effects of TNF-α on Senescent human dental pulp cells. International conference

    Nozu A, Hamano S, Tomokiyo A, Hasegawa D, Yoshida S, Sugii H, Mitarai H, Ipposhi K, Wada N, Maeda H

    Kyudai Oral Bioscience & OBT Research Center Joint International Symposium 2019  2019.3 

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    Event date: 2019.3

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Semaphorin3Aによる修復象牙質形成過程へのSonic hedgehogシグナルの関与

    吉田晋一郎、糸山知宏、長谷川大学、有馬麻衣、友清淳、濱野さゆり、杉井英樹、野津葵、和田尚久、前田英史

    日本歯科保存学会2018年度秋季学術大会(第149回)  2018.11 

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    Event date: 2018.11

    Language:Japanese  

    Country:Japan  

  • ヒト歯髄細胞の象牙芽細胞様分化におよぼすsFRP1の影響について

    一法師啓太、友清淳、長谷川大学、濱野さゆり、吉田晋一郎、杉井英樹、有馬麻衣、野津葵、和田尚久、前田英史

    日本歯科保存学会2018年度秋季学術大会(第149回)  2018.11 

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    Event date: 2018.11

    Language:Japanese  

    Country:Japan  

  • 線維芽細胞における仮足形成因子の探索

    祐田明香、和田尚久

    日本歯科保存学会2018年度秋季学術大会(第149回)  2018.11 

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    Event date: 2018.11

    Language:Japanese  

    Country:Japan  

  • GDNFは未分化なヒト歯根膜細胞のシュワン細胞分化を誘導する

    糸山知宏、吉田晋一郎、友清淳、長谷川大学、濱野さゆり、杉井英樹、有馬麻衣、野津葵、和田尚久、前田英史

    日本歯科保存学会2018年度秋季学術大会(第149回)  2018.11 

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    Event date: 2018.11

    Language:Japanese  

    Country:Japan  

  • 未分化なヒト歯根膜クローン細胞株およびハイドロキシアパタイト焼成体を用いた人工歯作製について

    小野太雅、友清淳、長谷川大学、濱野さゆり、吉田晋一郎、杉井英樹、有馬麻衣、小川真里奈、野津葵、和田尚久、前田英史

    日本歯科保存学会2018年度秋季学術大会(第149回)  2018.11 

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    Event date: 2018.11

    Language:Japanese  

    Country:Japan  

  • LGR4が未分化なヒト歯根膜細胞の増殖能、走化性および骨芽細胞様分化に及ぼす影響

    有馬麻衣、長谷川大学、吉田晋一郎、御手洗裕美、友清淳、濱野さゆり、杉井英樹、和田尚久、前田英史

    日本歯科保存学会2018年度秋季学術大会(第149回)  2018.11 

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    Event date: 2018.11

    Language:Japanese  

    Country:Japan  

  • インレーの再製を通して治療計画の立案について学んだ1症例

    阿潟濵陽子,伊吹禎一,田中華奈,和田尚久

    第11回日本総合歯科学会学術大会  2018.10 

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    Event date: 2018.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:鹿児島県歯科医師会館(鹿児島市)   Country:Japan  

  • Effects of dopamine on odontoblastic differentiation. International conference

    Fujino S, Hamano S, Tomokiyo A, Hasegawa D, Yoshida S, Sugii H, Washio A, Wada N, Kitamura C, Maeda H.

    The 11th IFEA World Endodontic Congress  2018.10 

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    Event date: 2018.10

    Language:English  

    Country:Korea, Republic of  

  • 九州大学病院歯科医師臨床研修における周術期口腔機能管理研修について

    寳田貫、稲井裕子、山添淳一、和田尚久

    第37回日本歯科医学教育学会学術大会  2018.7 

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    Event date: 2018.7

    Language:Japanese  

    Venue:奥羽大学(郡山市)   Country:Japan  

  • R-spondin2 Enhances Osteoblastic Differentiation of Immature Human Periodontal Ligament Cells. International conference

    Arima M, Hasegawa D, Yoshida S, Mitarai H, Tomokiyo A, Hamano S, Sugii H, Wada N, Maeda H

    96th General Session & Exhibition of the International Association for Dental Research (IADR)  2018.7 

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    Event date: 2018.7

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Venue:London, England   Country:United Kingdom  

  • Discoloration of White Mineral Trioxide Aggregate Immersed in Various Solutions. International conference

    Tomokiyo A, Hamano S, Hasegawa D, Sugii H, Yoshida S, Mitarai H, Sonoda M, Nozu A, Wada N, Maeda H

    96th General Session & Exhibition of the International Association for Dental Research (IADR)  2018.7 

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    Event date: 2018.7

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Venue:London, England   Country:United Kingdom  

  • Odontoblastic differentiation of senescence dental pulp cells treated by TNF-α. International conference

    Nozu A, Hamano S, Tomokiyo A, Hasegawa D, Sugii H, Yoshida S, Mitarai H, Taniguchi S, Wada N, Maeda H

    96th General Session & Exhibition of the International Association for Dental Research (IADR)  2018.7 

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    Event date: 2018.7

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Venue:London, England   Country:United Kingdom  

  • Nano Hydroxyapatite含有4-META/MMA-TBBレジンがヒト歯髄幹細胞に及ぼす影響について

    吉田晋一郎、杉井英樹、友清淳、長谷川大学、糸山知宏、野津葵、有馬麻衣、濱野さゆり、御手洗裕美、和田尚久、前田英史

    第39回日本歯内療法学会学術大会  2018.7 

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    Event date: 2018.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡国際会議場(福岡市)   Country:Japan  

  • 象牙芽細胞分化に及ぼすドーパミンの影響について

    藤野翔香、濱野さゆり、友清淳、長谷川大学、吉田晋一郎、杉井英樹、鷲尾絢子、御手洗裕美、野津葵、有馬麻衣、和田尚久、北村知昭、前田英史

    第39回日本歯内療法学会学術大会  2018.7 

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    Event date: 2018.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡国際会議場(福岡市)   Country:Japan  

  • 早期口腔機能回復を目的とした術者主導の平均的両側性平衡が付与された歯列を複製して行う治療用義歯作製法

    長田耕一郎、山添淳一、湯川綾美、和田尚久

    日本老年歯科医学会第29回学術大会  2018.6 

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    Event date: 2018.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:きゅりあん(品川区立総合区民会館)(東京都)   Country:Japan  

  • 歯牙腫におけるWnt/β-cateninシグナルの活性化は軸索伸張制御因子(Sema3A)を介して増殖を制御する

    藤井慎介、永田健吾、清島保、和田尚久

    第107回日本病理学会総会  2018.6 

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    Event date: 2018.6

    Language:Japanese  

    Country:Japan  

  • 新規幹細胞関連因子MESTがヒト歯根膜細胞の幹細胞転換に及ぼす影響

    長谷川大学、長谷川佳那、御手洗裕美、有馬麻衣、濱野さゆり、吉田晋一郎、友清淳、杉井英樹、和田尚久、清島保、前田英史

    日本歯科保存学会2018年度春季学術大会(第148回)  2018.6 

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    Event date: 2018.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:横浜みなとみらいホール(横浜市)   Country:Japan  

  • 老化したヒト歯髄細胞の象牙芽細胞様分化におよぼすTNF-αの影響について

    野津葵、濱野さゆり、友清淳、長谷川大学、吉田晋一郎、杉井英樹、御手洗裕美、一法師啓太、和田尚久、前田英史

    日本歯科保存学会2018年度春季学術大会(第148回)  2018.6 

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    Event date: 2018.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:横浜みなとみらいホール(横浜市)   Country:Japan  

  • Activin Aがヒト歯根膜細胞およびヒト前骨芽細胞の骨芽細胞様分化に及ぼす影響について

    杉井英樹、友清淳、濱野さゆり、長谷川大学、吉田晋一郎、御手洗裕美、野津葵、有馬麻衣、糸山知宏、小野太雅、藤野翔香、一法師啓太、和田尚久、前田英史

    日本歯科保存学会2018年度春季学術大会(第148回)  2018.6 

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    Event date: 2018.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:横浜みなとみらいホール(横浜市)   Country:Japan  

  • Basic Fibroblast Growth FactorおよびephrinB2がヒト歯根膜細胞の増殖に及ぼす影響について

    小野太雅、友清淳、長谷川大学、濱野さゆり、吉田晋一郎、杉井英樹、御手洗裕美、有馬麻衣、野津葵、和田尚久、前田英史

    日本歯科保存学会2018年度春季学術大会(第148回)  2018.6 

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    Event date: 2018.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:横浜みなとみらいホール(横浜市)   Country:Japan  

  • 認知症を伴った舌癌患者に対し周術期口腔機能管理を通して経口摂取支援を行った1症例

    湯川綾美、山添淳一、和田尚久

    第15回日本口腔ケア学会総会・学術大会  2018.4 

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    Event date: 2018.4

    Language:Japanese  

    Venue:福岡国際会議場(福岡市)   Country:Japan  

  • 当院新人看護師の技術習得度調査にもとづく口腔ケア実践研修の効果

    斧みなみ、中村次代、菊村里香、疋田春奈、村田彩、安波亜理紗、平野菜々美、稲井裕子、二木寿子、柏﨑晴彦、和田尚久

    第15回日本口腔ケア学会総会・学術大会  2018.4 

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    Event date: 2018.4

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:福岡国際会議場(福岡市)   Country:Japan  

  • 周術期口腔機能管理による術後呼吸器合併症の抑制効果―肺切除術患者における検討―

    寶田貫、稲井裕子、和田尚久

    第15回日本口腔ケア学会総会・学術大会  2018.4 

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    Event date: 2018.4

    Language:Japanese  

    Venue:福岡国際会議場(福岡市)   Country:Japan  

  • R-spondin2 enhances osteogenesis of immature human periodontal ligament cells through the canonical Wnt signaling pathway.

    Arima M, Hasegawa D, Yoshida S, Mitarai H, Tomokiyo A, Hamano S, Sugii H, Wada N, Maeda H

    Kyudai Oral Bioscience 2018 –Health Longevity from Oral Brain Science: From Basic to Clinical Research-  2018.2 

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    Event date: 2018.2 - 2017.2

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Collaboration Station Ⅰ, 2F Audiovisual Hall Kyushu University, Fukuoka   Country:Japan  

  • 歯牙腫においてWnt/β-cateninシグナルの活性化は軸索伸張制御因子を介して増殖を制御する

    藤井慎介、和田尚久

    第94回九大病理研究会プログラム  2017.12 

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    Event date: 2017.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:九州大学病院地区 コラボステーションⅠ(福岡市)   Country:Japan  

  • Activin A reversely works on the osteoblastic differentiation in human pre-osteoblastic cells and periodontal ligament cells.

    Sugii H, Tomokiyo A, Hamano S, Hasegawa D, Yoshida S, Mitarai H, Nozu A, Arima M, Itoyama T, Ono T, Fujino S, Ipposhi K, Wada N, Maeda H

    The 65th Annual Meeting of Japanese Association for Dental Research (JADR)  2017.11 

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    Event date: 2017.11

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Venue:Showa University, Tokyo   Country:Japan  

  • 肥大型心筋症を認識していなかった患者に対し、モニタリングにより心電図波形の異常を発見した1症例

    衛藤希、山添淳一、赤木裕美、田上裕梨、武末康寛、和田尚久

    第10回日本総合歯科学会総会・学術大会  2017.11 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:アートホテル新潟駅前,新潟大学 駅南キャンパス ときめいと(新潟市)   Country:Japan  

  • 九州大学病院周術期口腔ケアセンターにおける周術期口腔管理研修

    寳田貫、稲井裕子、大山恵子、和田尚久

    第10回日本総合歯科学会総会・学術大会  2017.11 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:アートホテル新潟駅前,新潟大学 駅南キャンパス ときめいと(新潟市)   Country:Japan  

  • 作成した図を用いて治療計画を説明しラポール形成に役立てた症例

    三田公麿、伊吹禎一、和田尚久

    第10回日本総合歯科学会総会・学術大会  2017.11 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:アートホテル新潟駅前,新潟大学 駅南キャンパス ときめいと(新潟市)   Country:Japan  

  • 近い将来咬合崩壊を起こしそうな高齢患者の治療経験

    佐野大成、伊吹禎一、和田尚久

    第10回日本総合歯科学会総会・学術大会  2017.11 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:アートホテル新潟駅前,新潟大学 駅南キャンパス ときめいと(新潟市)   Country:Japan  

  • 歯牙腫におけるWnt/β-cateninシグナルの活性化は軸索伸張制御因子を介して増殖を制御する

    藤井 慎介、永田 健吾、清島 保、和田 尚久

    第63回日本病理学会秋期特別総会  2017.11 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:日本教育会館(東京都)   Country:Japan  

  • Nano Hydroxiapatite 含有4-META/MMA-TBBレジンがヒト歯髄幹細胞に及ぼす影響について

    吉田晋一郎、杉井英樹、友清淳、長谷川大学、糸山知宏、御手洗裕美、有馬麻衣、濱野さゆり、野津葵、和田尚久、前田英史

    日本歯科保存学会2017年度秋季学術大会(第147回)第19回日韓歯科保存学会学術大会  2017.10 

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    Event date: 2017.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:マリオス(盛岡市)   Country:Japan  

  • R-spondin2はカノニカルWntシグナルを介して未分化なヒト歯根膜細胞の骨芽細胞様分化を促進する

    有馬麻衣、長谷川大学、吉田晋一郎、御手洗裕美、友清淳、濱野さゆり、杉井英樹、和田尚久、前田英史

    日本歯科保存学会2017年度秋季学術大会(第147回)第19回日韓歯科保存学会学術大会  2017.10 

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    Event date: 2017.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:マリオス(盛岡市)   Country:Japan  

  • 「周術期口腔機能管理の基本概念の理解」を目標とするチーム基盤型学習(TBL)のユニット設計

    寳田貫、和田尚久、築山能大、三木洋一郎

    第36回日本歯科医学教育学会総会および学術大会  2017.7 

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    Event date: 2017.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:松本市中央会館(松本市)   Country:Japan  

  • ワークショップ「倫理的検討事例を用いたプロフェッショナリズム教育の展開」報告

    平田創一郎、木尾哲朗、尾崎哲則、樫則章、角忠輝、山本龍生、和田尚久 酒寄孝治、平田幸夫、俣木志朗

    第36回日本歯科医学教育学会総会および学術大会  2017.7 

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    Event date: 2017.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:松本市中央会館(松本市)   Country:Japan  

  • 知的障害を伴った舌癌患者に対し周術期口腔機能管理を通して経口摂取支援を行った1症例

    湯川綾美, 山添 淳一, 和田 尚久

    日本老年歯科医学会第28回学術大会  2017.6 

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    Event date: 2017.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:名古屋国際会議場(名古屋市)   Country:Japan  

  • 熊本地震の震災慢性期における口腔機能支援チームの活動-要介護高齢者の震災関連死を予防した1症例-

    塚本 葉子, 山添 淳一, 和田 尚久

    日本老年歯科医学会第28回学術大会  2017.6 

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    Event date: 2017.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:名古屋国際会議場(名古屋市)   Country:Japan  

  • 脊髄小脳変性症で重度フレイルと思われた患者に義歯を使用させることで本来のフレイル段階が判明した一症例

    長田耕一郎, 山添 淳一, 和田 尚久

    日本老年歯科医学会第28回学術大会  2017.6 

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    Event date: 2017.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋国際会議場(名古屋市)   Country:Japan  

  • Tenomodulinがヒト歯根膜細胞の機能維持に及ぼす影響について

    長谷川 大学, 和田 尚久, 有馬麻衣, 吉田 晋一郎, 友清 淳, 濱野 さゆり, 御手洗 裕美, 前田 英史

    第146回日本歯科保存学会春季学術大会  2017.6 

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    Event date: 2017.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:リンクステーションホール青森(青森市)   Country:Japan  

  • 新規象牙質細胞マーカーとしてのチロシン水酸化酵素の可能性

    藤野翔香, 濱野 さゆり, 芹田俊, 友清 淳, 長谷川 大学, 吉田 晋一郎, 水町 博之, 御手洗 裕美, 和田 尚久, 清島 保, 前田 英史

    第146回日本歯科保存学会春季学術大会  2017.6 

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    Event date: 2017.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:リンクステーションホール青森(青森市)   Country:Japan  

  • 熊本地震の歯科保健医療支援活動における支援撤退時口腔機能情報共有ツールの検討

    山添 淳一, 和田 尚久, 塚本 葉子, 上野真智子, 中村 誠司

    第14回日本口腔ケア学会総会・学術大会  2017.4 

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    Event date: 2017.4

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:沖縄コンベンションセンター(宜野湾市)   Country:Japan  

  • The Expression Pattern of the FRG1 in the Mouse Developing Tooth Germ. International conference

    Hiroko Wada, Kana Hasegawa, Kengo Nagata, Naohisa Wada, Y. Mikami, Hidetaka Sakai, Tamotsu Kiyoshima

    95th General Session & Exhibition of the International Association for Dental Research (IADR)  2017.3 

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    Event date: 2017.3

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Venue:San Francisco, Calif.   Country:United States  

  • Transgelin Mediates the proliferation of human periodontal ligament cells induced by TGF-β1. International conference

    Hiromi Mitarai, Naohisa Wada, Daigaku Hasegawa, Shinichiro Yoshida, Mai Sonoda, Atsushi Tomokiyo, Sayuri Hamano, Suguru Serita, Hideki Mizumachi, Hidefumi Maeda

    Kyudai Oral Bioscience 2017  2017.2 

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    Event date: 2017.2

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Fukuoka   Country:Japan  

  • 数回の治療中断を経て即時義歯装着に至った症例

    戸髙 結衣, 伊吹 禎一, 角 義久, 和田 尚久

    第9回日本総合歯科学会  2016.11 

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    Event date: 2016.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:岡山大学鹿田キャンパスJ Hall記念会館(岡山市)   Country:Japan  

  • 重度の咬耗により補綴困難となった症例に対し咬合挙上を行った症例

    赤木 裕美, 王丸 寛美, 和田 尚久

    第9回日本総合歯科学会  2016.11 

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    Event date: 2016.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:岡山大学鹿田キャンパスJ Hall記念会館(岡山市)   Country:Japan  

  • 熊本地震の震災慢性期における歯科保健医療支援活動~歯科医師、歯科衛生士、言語聴覚士による協働支援~

    塚本 葉子, 山添 淳一, 坂本瑞樹, 上野真智子, 西山伸二, 中里一成, 下池光, 近藤泰子, 横山茂幹, 橋口智英, 和田 尚久, 中村 誠司

    リハビリテーション•ケア合同研究大会 茨城2016  2016.10 

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    Event date: 2016.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:つくば国際会議場   Country:Japan  

  • 紫外線照射が歯内疾患関連細菌および歯髄細胞に及ぼす影響

    原口 晃, 吉田 晋一郎, 竹下 正章, 角 保徳, 西村 英紀, 前田 英史, 和田 尚久

    第145回日本歯科保存学会秋季学術大会  2016.10 

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    Event date: 2016.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:キッセイ文化ホール(松本市)   Country:Japan  

  • R-spondin2が未分化なヒト歯根膜細胞の線維芽細胞様分化に及ぼす影響

    園田 麻衣, 長谷川 大学, 和田 尚久, 吉田 晋一郎, 御手洗 裕美, 友清 淳, 濱野 さゆり, 前田 英史

    第145回日本歯科保存学会秋季学術大会  2016.10 

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    Event date: 2016.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:キッセイ文化ホール(松本市)   Country:Japan  

  • Mineral Trioxide Aggregateの色調変化に関する比較分析

    友清 淳, 和田 尚久, 濱野 さゆり, 長谷川 大学, 杉井 英樹, 吉田 晋一郎, 芹田 俊, 御手洗 裕美, 水町 博之, 前田 英史

    第145回日本歯科保存学会秋季学術大会  2016.10 

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    Event date: 2016.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:キッセイ文化ホール(松本市)   Country:Japan  

  • 歯根膜および皮膚由来ヒト人口多能性幹細胞(iPSC)を用いた神経堤細胞様細胞の樹立とキャラクタライゼーション

    友清 淳, 和田 尚久, 濱野 さゆり, 長谷川 大学, 杉井 英樹, 吉田 晋一郎, 前田 英史

    第23回日本歯科医学会総会  2016.10 

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    Event date: 2016.10

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:福岡国際会議場(福岡市)   Country:Japan  

  • ヒト歯髄細胞の老化と象牙芽細胞様分化におよぼすTNF-alphaの影響について

    野津葵, 友清 淳, 長谷川 大学, 濱野 さゆり, 吉田 晋一郎, 芹田俊, 御手洗裕美, 和田 尚久, 前田 英史

    第37回日本歯内療法学会学術大会  2016.7 

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    Event date: 2016.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:ウインクあいち(名古屋市)   Country:Japan  

  • 歯科外来受診患者における睡眠時無呼吸に関する調査(第2報)

    王丸 寛美, 津田緩子, 和田 尚久

    日本補綴歯科学会第125回学術大会  2016.7 

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    Event date: 2016.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:石川県立音楽堂(金沢市)   Country:Japan  

  • 全国歯科大学・歯学部における「2013年度版 よき歯科医師になるための20の質問 倫理的検討事例集」の利用状況

    平田創一郎, 酒寄孝治, 山本龍生, 木尾哲郎, 尾崎哲則, 樫則章, 角忠輝, 和田 尚久, 平田幸夫

    第35回日本歯科医学教育学会総会および学術大会  2016.7 

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    Event date: 2016.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:大阪大学コンベンションセンター   Country:Japan  

  • 意識レベル低下を伴った進行性悪性髄膜腫患者に対する口腔ケア介入の1例

    菊村里香, 二木 寿子, YUKO INAI, 中村次代, 塚本葉子, 和田 尚久, 中村 誠司

    第13回日本口腔ケア学会総会・学術大会  2016.4 

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    Event date: 2016.4

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:京葉銀行文化プラザ(千葉市)   Country:Japan  

  • 九州大学病院周術期口腔ケアセンターにおける歯科衛生士の取り組み

    村田彩, 中村次代, 菊村里香, 塚本葉子, 溜池みなみ, 疋田春奈, 寳田 貫, 二木 寿子, YUKO INAI, 井上 良介, 和田 尚久, 中村 誠司

    第13回日本口腔ケア学会総会・学術大会  2016.4 

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    Event date: 2016.4

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:京葉銀行文化プラザ(千葉市)   Country:Japan  

  • 術前歯科未介入の周術期患者の状況についての分析

    塚本葉子, YUKO INAI, 二木 寿子, 寳田 貫, 中村次代, 菊村里香, 村田彩, 溜池みなみ, 疋田春奈, 和田 尚久, 中村 誠司

    第13回日本口腔ケア学会総会・学術大会  2016.4 

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    Event date: 2016.4

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:京葉銀行文化プラザ(千葉市)   Country:Japan  

  • 外科処置を伴わない重度慢性歯周病患者におけるBP 製剤の影響

    佐土原 祥伍, 王丸 寛美, 和田 尚久

    第8回日本総合歯科学会総会・学術大会  2015.11 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:日本歯科大学生命歯学部(東京都)   Country:Japan  

  • 九州大学病院における臨床研修歯科医のための周術期口腔管理研修の試み

    YUKO INAI, 林 武文, 寳田 貫, 大山 恵子, 王丸 寛美, 和田 尚久

    第8回日本総合歯科学会総会・学術大会  2015.11 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:日本歯科大学生命歯学部(東京都)   Country:Japan  

  • ヒト歯髄細胞の石灰化におけるCalcium-sensing receptorの関与について

    水町博之, 友清 淳, 長谷川 大学, 濱野 さゆり, 吉田晋一郎, 杉井英樹, 芹田俊, 御手洗裕美, 和田 尚久, 前田 英史

    第143回日本歯科保存学会秋季学術大会  2015.11 

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    Event date: 2015.11

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:文京シビックホール(東京都)   Country:Japan  

  • 九州大学病院における周術期口腔ケアセンターの設立と実績について

    寳田 貫, 二木 寿子, YUKO INAI, 井上 良介, 菊村里香, 塚本葉子, 中村次代, 疋田春奈, 和田 尚久, 中村 誠司

    第12回日本口腔ケア学会総会・学術大会  2015.6 

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    Event date: 2015.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:海峡メッセ下関(下関市)   Country:Japan  

  • 歯根膜および皮膚由来ヒト人口多能性幹細胞(iPSC)を用いた神経堤細胞株細胞の樹立とその表現型の比較

    友清 淳, 前田 英史, 和田 尚久, 門野内 聡, 濱野 さゆり, 長谷川 大学, 祐田 明香, 赤峰 昭文

    第142回日本歯科保存学会春季学術大会  2015.6 

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    Event date: 2015.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:北九州国際会議場(北九州市)   Country:Japan  

  • Effect of β2 Adrenergic Receptor on Human Periodontal Ligament Cells. International conference

    Sayuri Hamano, Hidefumi Maeda, Daigaku Hasegawa, Monnouchi Satoshi, Naohisa Wada, Atsushi Tomokiyo, Asuka Yuda, Hideki Sugii, Shinichiro Yoshida, Akifumi Akamine

    93rd General Session & Exhibition of the International Association for Dental Research (IADR)  2015.3 

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    Event date: 2015.3

    Language:English  

    Venue:Boston, Mass.   Country:United States  

  • Collaborative activity of CTGF/CCN2 and TGF-β1 on osteogenesis and fibrogenesis. International conference

    Asuka Yuda, Hidefumi Maeda, Fujii Shinsuke, Monnouchi Satoshi, Naohisa Wada, Atsushi Tomokiyo, Sayuri Hamano, Hideki Sugii, Daigaku Hasegawa, Shinichiro Yoshida, Akifumi Akamine

    93rd General Session & Exhibition of the International Association for Dental Research (IADR)  2015.3 

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    Event date: 2015.3

    Language:English  

    Venue:Boston, Mass.   Country:United States  

  • Effects of Activin A on the phenotypic properties of human periodontal ligament cells. International conference

    Hideki Sugii, Hidefumi Maeda, Atsushi Tomokiyo, Naohisa Wada, Monnouchi Satoshi, Daigaku Hasegawa, Sayuri Hamano, Asuka Yuda, Shinichiro Yoshida, S. Serita, H. Mizumachi, Hiromi Mitarai, Akifumi Akamine

    Kyudai Oral Bioscience 2015 - 9th International Symposium -  2015.2 

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    Event date: 2015.2

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Fukuoka Recent Hotel, Fukuoka   Country:Japan  

  • 骨芽細胞様分化におけるActivinAの作用は前骨芽細胞と歯根膜細胞とで相反する

    杉井英樹, 前田 英史, 友清 淳, 和田 尚久, 門野内 聡, 長谷川大学, 濱野さゆり, 祐田明香, 赤峰 昭文

    第141回日本歯科保存学会秋季学術大会  2014.6 

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    Event date: 2014.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:山形テルサ(山形市)   Country:Japan  

  • β2アドレナリン受容体の作動薬および非作動薬がヒト歯根膜細胞に及ぼす影響

    濱野さゆり, 前田 英史, 長谷川 大学, 友清 淳, 和田 尚久, 門野内 聡, 祐田明香, 杉井英樹, 赤峰 昭文

    第140回日本歯科保存学会春季学術大会  2014.6 

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    Event date: 2014.6

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:滋賀県立芸術劇場(大津市)   Country:Japan  

  • Wnt5aがヒト歯根膜細胞に及ぼす影響について

    長谷川大学, 和田 尚久, 前田 英史, 友清淳, 門野内 聡, 郡 勝明, 吉田晋一郎, 寺松陽子, 濱野さゆり, 祐田明香, 杉井英樹, 赤峰 昭文

    第139回日本歯科保存学会秋季学術大会  2013.10 

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    Event date: 2013.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:秋田県総合生活文化会館(秋田市)   Country:Japan  

  • CTGF/CCN2が未分化なヒト歯根膜細胞株の骨芽細胞様分化に及ぼす影響

    祐田明香, 前田 英史, 藤井慎介, 門野内 聡, 山本 直秀, 和田 尚久, 友清 淳, 郡 勝明, 寺松陽子, 濱野さゆり, 長谷川大学, 赤峰 昭文

    第5回日本CCNファミリー研究会  2013.9 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岡山コンベンションセンター(岡山市)   Country:Japan  

  • ActivinAがヒト歯根膜細胞に及ぼす影響について

    杉井英樹, 前田 英史, 友清 淳, 山本直秀, 郡 勝明, 和田 尚久, 門野内 聡, 濱野さゆり, 長谷川大学, 祐田明香, 寺松陽子, 河野 清美, 吉田晋一郎, 赤峰 昭文

    第138回日本歯科保存学会秋季学術大会  2013.6 

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    Event date: 2013.6

    Language:Japanese  

    Venue:福岡国際会議場(福岡市)   Country:Japan  

  • 九州大学病院歯周病科歯内治療科における周術期口腔機能管理への取り組み

    作原涼子, 立野麗子, 中村次代, 岡田英里, 村田彩, 和田 尚久, 藤瀬 修

    第10回日本口腔ケア学会総会・学術大会  2013.6 

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    Event date: 2013.6

    Language:Japanese  

    Venue:九州大学医学部百年講堂(福岡市)   Country:Japan  

  • Localization of βig-h3 and Collagen type I in dental pulp. International conference

    Monnouchi Satoshi, Hidefumi Maeda, naohisa wada, Asuka Yuda, Akifumi Akamine

    The 9th World Endodontic Congress  2013.5 

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    Event date: 2013.5

    Language:English  

    Venue:Tokyo International Forum, Tokyo   Country:Japan  

  • Molecular assessment of bacterial community in persistent apical periodontitis granulomatous tissue: 16S rDNA clone library analysis. International conference

    Zakaria MN, Hidefumi Maeda, naohisa wada, Akifumi Akamine

    The 9th World Endodontic Congress  2013.5 

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    Event date: 2013.5

    Language:English  

    Venue:Tokyo International Forum, Tokyo   Country:Japan  

  • Evaluation of Calcium-modified 4-META/MMA-TBB resin on its bioactive feature. International conference

    Hidefumi Maeda, 郡 勝明, naohisa wada, Monnouchi Satoshi, Yoko Teramatsu, Kono Kiyomi, Akifumi Akamine

    The 9th World Endodontic Congress  2013.5 

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    Event date: 2013.5

    Language:English  

    Venue:Tokyo International Forum, Tokyo   Country:Japan  

  • CaCl2-added resin exerts bioactive effects on periodontal ligament cells International conference

    Hidefumi Maeda, Atsushi Tomokiyo, Katsuaki Koori, Naohide Yamamoto, naohisa wada, Monnouchi Satoshi, Saori Hamano, Asuka Yuda, Akifumi Akamine

    91st General Session & Exhibition of the IADR  2013.3 

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    Event date: 2013.3

    Language:English  

    Venue:Seattle   Country:United States  

  • Extracellular calcium regulates osteoblastic differentiation of undifferentiated PDL cells International conference

    Katsuaki Koori, Hidefumi Maeda, naohisa wada, Atsushi Tomokiyo, Monnouchi Satoshi, Naohide Yamamoto, Akifumi Akamine

    91st General Session & Exhibition of the IADR  2013.3 

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    Event date: 2013.3

    Language:English  

    Venue:Seattle   Country:United States  

  • Roles of glial cell-derived neurotrophic factor in periodontal ligament cells International conference

    Naohide Yamamoto, Hidefumi Maeda, Atsushi Tomokiyo, naohisa wada, Monnouchi Satoshi, Katsuaki Koori, Akifumi Akamine

    91st General Session & Exhibition of the IADR  2013.3 

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    Event date: 2013.3

    Language:English  

    Venue:Seattle   Country:United States  

  • beta2アドレナリン受容体の非作動薬であるPropanolがヒト歯根膜細胞の骨芽細胞分化に及ぼす影響.

    濱野さゆり, 前田 英史, 友清 淳, 山本直秀, 門野内 聡, 和田 尚久, 河野 清美, 郡勝明, 寺松陽子, 長谷川大学, 祐田明香, 赤峰 昭文

    第137回日本歯科保存学会秋季学術大会  2012.11 

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    Event date: 2012.11

    Language:Japanese  

    Venue:広島国際会議場(広島市)   Country:Japan  

  • CTGFが未分化なヒト歯根膜細胞株の骨芽細胞分化に及ぼす影響

    祐田明香, 前田 英史, 藤井 慎介, 門野内 聡, 山本直秀, 和田 尚久, 友清 淳, 郡勝明, 河野 清美, 寺松陽子, 濱野さゆり, 長谷川大学, 赤峰 昭文

    第137回日本歯科保存学会秋季学術大会  2012.11 

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    Event date: 2012.11

    Language:Japanese  

    Venue:広島国際会議場(広島市)   Country:Japan  

  • ダイオキシン関連物質がヒト歯根膜細胞の骨芽細胞分化に及ぼす影響

    門野内 聡, 前田 英史, 友清 淳, 和田 尚久, 河野 清美, 郡 勝明, 山本 直秀, 寺松 陽子, 赤峰 昭文

    第136回日本歯科保存学会春季学術大会  2012.6 

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    Event date: 2012.6

    Language:Japanese  

    Venue:沖縄コンベンションセンター(宜野湾市)   Country:Japan  

  • ベンゾピレンがヒト歯根膜細胞の骨芽細胞分化に及ぼす影響

    門野内 聡, 前田 英史, 友清 淳, 和田 尚久, 河野 清美, 郡 勝明, 山本 直秀, 寺松 陽子, 赤峰 昭文

    平成24年度厚生労働省全国油症治療研究班会議  2012.6 

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    Event date: 2012.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:ホテルレガロ福岡(福岡)   Country:Japan  

  • Potency of Mechanical Load in Differentiation of Periodontal Ligament Stem Cells. Invited International conference

    Maeda H, Monnouchi S, Fujii S, Tomokiyo A, Wada N, Akamine A.

    4th Annual World Congress of Regenerative Medicine & Stem Cell 2011.  2011.11 

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    Event date: 2011.11

    Presentation type:Oral presentation (general)  

    Venue:Beijing   Country:China  

  • EGFがヒト歯根膜細胞に及ぼす影響について

    寺松 陽子、前田 英史、友清 淳、門野内 聡、山本 直秀、和田 尚久、藤井 慎介、河野 清美、郡 勝明、赤峰昭文

    第135回日本歯科保存学会秋季学術大会  2011.10 

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    Event date: 2011.10

    Presentation type:Oral presentation (general)  

    Venue:大阪市   Country:Japan  

  • Stretched periodontal ligament cells up-regulate Interleukin-11 to regulate osteoblastic metabolism.

    Monnouchi S, Maeda H, Tomokiyo A, Yamamoto N, Teramatsu Y, Wada N, Kono K, Koori K, Akamine A.

    The 59th Annual Meeting of Japanese Association for Dental Research(JADR)  2011.10 

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    Event date: 2011.10

    Venue:広島市   Country:Japan  

  • Passive Ultrasonic Irrigationによる根管洗浄の効果-SEM観察-

    木原智子、前田英史、郡勝明、寺松陽子、和田尚久、藤井慎介、友清淳、門野内聡、河野清美、山本直秀、後藤康治、赤峰昭文

    第134回日本歯科保存学会春季学術大会  2011.6 

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    Event date: 2011.6

    Venue:東京都   Country:Japan  

  • ヒト歯根膜細胞への進展刺激はInterleukin-11の発現を促進する

    門野内聡、前田英史、山本直秀、藤井慎介、友清淳、和田尚久、河野清美、郡勝明、寺松陽子、木原智子、赤峰昭文

    第134回日本歯科保存学会春季学術大会  2011.6 

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    Event date: 2011.6

    Venue:東京都   Country:Japan  

  • The roles of stretch loading in human periodontal ligament cells International conference

    Satoshi Monnouchi, Hidefumi Maeda, Shinsuke Fujii, Atsushi Tomokiyo, Naohisa Wada, Kiyomi Kona, Akifumi Akamine

    The 6th international joint symposium on “dental and craniofacial morphogenesis and tissue regeneration” and “oral health science”  2011.3 

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    Event date: 2011.3

    Presentation type:Oral presentation (general)  

    Venue:Fukuoka   Country:Japan  

  • Contribution of mechanical load to the regeneration of human periodontal ligament tissues Invited International conference

    Maeda H, Monnouchi M, Fujii S, Tomokiyo A, Wada N, Akamine A

    International Biomedical and Technology & Healthcare Management Summit  2010.11 

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    Event date: 2010.11 - 2011.11

    Presentation type:Oral presentation (general)  

    Venue:Jiangyin   Country:China  

  • ヒト歯根膜および歯髄細胞の免疫抑制特性に関する研究

    和田尚久、前田英史、藤井慎介、友清淳、赤峰昭文

    第133回日本歯科保存学会秋季学術大会  2010.10 

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    Event date: 2010.10

    Presentation type:Oral presentation (general)  

    Venue:岐阜市   Country:Japan  

  • 未分化なヒト歯根膜細胞株の分化に及ぼすカルシウムの影響について

    郡勝明、前田英史、藤井慎介、友清淳、門野内聡、和田尚久、河野清美、山本直秀、寺松陽子、木原智子、赤峰昭文

    第133回日本歯科保存学会秋季学術大会  2010.10 

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    Event date: 2010.10

    Presentation type:Oral presentation (general)  

    Venue:岐阜市   Country:Japan  

  • GDNFがヒト歯根膜細胞の走化性に及ぼす影響について

    山本直秀、前田英史、友清淳、藤井慎介、和田尚久、門野内聡、河野清美、郡勝明、寺松陽子、木原智子、赤峰昭文

    第133回日本歯科保存学会秋季学術大会  2010.10 

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    Event date: 2010.10

    Presentation type:Oral presentation (general)  

    Venue:岐阜市   Country:Japan  

  • bFGFが未分化なヒト歯根膜細胞株の線維芽細胞様分化に及ぼす影響について

    河野清美、前田英史、和田尚久、藤井慎介、友清淳、門野内聡、山本直秀、郡勝明、寺松陽子、木原智子、赤峰昭文

    第133回日本歯科保存学会秋季学術大会  2010.10 

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    Event date: 2010.10

    Presentation type:Oral presentation (general)  

    Venue:岐阜市   Country:Japan  

  • スーパーボンドシーラーの特性について -ヒト歯根膜細胞の増殖と分化に与える影響-

    前田英史、友清淳、郡勝明、藤井慎介、門野内聡、安田善之、山本直秀、堀清美、和田尚久、斎藤隆史、赤峰昭文

    第31回日本歯内療法学会学術大会  2010.7 

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    Event date: 2010.7

    Presentation type:Oral presentation (general)  

    Venue:東京都   Country:Japan  

  • What are the characteristics of PDL stem cells? Invited International conference

    Maeda H, Fujii S, Tomokiyo A, Wada N, Monnouchi S, Hori K, Koori K, Yamamoto N, Akamine A.

    The 5th International Symposium on "Dental and Craniofacial Morphogenesis and Tissue Regeneration: A View from Stem Cell Researches  2010.2 

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    Event date: 2010.2

    Presentation type:Oral presentation (general)  

    Venue:Fukuoka   Country:Japan  

  • AhRシグナルがヒト歯根膜細胞のコラーゲン代謝に及ぼす影響

    友清淳、前田英史、藤井慎介、和田尚久、門野内聡、堀清美、郡勝明、山本直秀、赤峰昭文

    第131回日本歯科保存学会秋季学術大会  2009.10 

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    Event date: 2009.10

    Venue:仙台   Country:Japan  

  • TGF-beta1がヒト歯根膜細胞および前駆細胞の増殖および分化に及ぼす影響

    藤井慎介、前田英史、友清淳、橋口勇、門野内聡、堀清美、和田尚久、赤峰昭文

    第130回日本歯科保存学会春季学術大会  2009.6 

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    Event date: 2009.6

    Venue:札幌   Country:Japan  

  • MTA stimulates BMP2 expression in human PDL cells International conference

    Maeda H, Tomokiyo A, Fujii S, Monnouchi S, Wada N, Hori K, Akamine A

    87th General Session & Exhibition of the IADR  2009.4 

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    Event date: 2009.4

    Venue:Miami   Country:United States  

    MTA stimulates BMP2 expression in human PDL cells

  • Expression and effects of TGFβ-1 in periodontal ligament tissue International conference

    Fujii S, Maeda H, Wada N, Tomokiyo A, Monnouchi S, Hori K, Akamine A

    87th General Session & Exhibition of the IADR  2009.4 

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    Event date: 2009.4

    Venue:Miami   Country:United States  

    Expression and effects of TGFβ-1 in periodontal ligament tissue

  • MTAはヒト歯根膜細胞のBMP2発現を誘導する

    前田英史、友清淳、藤井慎介、島一也、中野嗣久、和田尚久、門野内聡、堀清美、赤峰昭文

    日本歯科保存学会  2008.11 

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    Event date: 2008.11

    Venue:富山   Country:Japan  

  • Calcium-releasing Agent Exhibits Bioactive Effects in Endodontic Therapy Invited International conference

    MAEDA H, TOMOKIYO A, FUJII S, WADA N, MONNOUCHI S, HORI K, AKAMINE A

    2nd World Conference on Magic Bullets (Ehrlich II)  2008.10 

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    Event date: 2008.10

    Venue:Nurnberg   Country:Japan  

  • MTA induces mineralization of human periodontal ligament cells International conference

    Maeda H, Tomokiyo A, Fujii S, Wada N, Monnouchi S, Nakano T, Akamine A

    IADR/AADR/CADR  2008.7 

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    Event date: 2008.7

    Venue:Toronto   Country:Japan  

  • 多分化能を有するヒト歯根膜クローン細胞株が神経細胞分化及び細胞遊走に及ぼす影響

    友清淳、前田英史、藤井慎介、和田尚久、門野内聡、赤峰昭文

    日本歯科保存学会  2008.6 

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    Event date: 2008.6

    Venue:新潟市   Country:Japan  

  • MTAはヒト歯根膜細胞の骨芽細胞様分化を誘導する

    前田英史、中野嗣久、友清淳、藤井慎介、門野内聡、和田尚久、赤峰昭文

    日本歯科保存学会  2008.6 

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    Event date: 2008.6

    Venue:新潟市   Country:Japan  

  • A multipotent hPDL cell line promotes neurocytic differentiation and migration International conference

    Tomokiyo A, Maeda H, Fujii S, Wada N, Monnouchi S, Akamine A

    IADR/AADR/CADR  2008.6 

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    Event date: 2008.6

    Venue:Toronto   Country:Japan  

  • スメアクリーン洗浄後の根管象牙質のSEM観察像−超音波洗浄との比較−

    島一也、前田英史、後藤康治、畦森雅子、和田尚久、藤井慎介、友清淳、赤峰昭文

    第125回日本歯科保存学会秋季大会  2006.11 

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    Venue:鹿児島市   Country:Japan  

  • 水酸化カルシウム製材カルシペックスの組織親和性に関する研究

    橋口勇、前田英史、和田尚久、中野嗣久、中牟田博敬、赤峰昭文

    第108回日本歯科保存学会秋季大会  1998.6 

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    Country:Japan  

  • 歯根肉芽腫由来の線維芽細胞様細胞は石灰化能を有する

    前田英史、中牟田博敬、和田尚久、赤峰昭文

    第109回日本歯科保存学会秋季大会  1998.11 

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    Presentation type:Oral presentation (general)  

    Country:Japan  

  • 歯根膜細胞における破骨細胞形成抑制因子OPG/OCIFの発現

    和田尚久、前田英史、中牟田博敬、田邊一成、赤峰昭文

    第112回日本歯科保存学会秋季大会  2000.6 

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    Presentation type:Oral presentation (general)  

    Venue:大阪市   Country:Japan  

  • The expression of OPG/OCIF in human periodontal ligament cells. International conference

    Wada N, Maeda H, Tsuda E, Yano K, Nakamuta H, Akamine A

    79th General Session & Exhibition of the IADR  2001.6 

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    Presentation type:Symposium, workshop panel (public)  

    Venue:Chiba  

  • U-937-DE4細胞を用いたヒト破骨細胞様細胞形成系における転写因子Egr1の役割

    久木田敏夫、久木田明子、渡辺敏之、和田尚久、藤加寿子、飯島忠彦

    第43回歯科基礎医学会  2001.9 

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    Venue:大宮市  

  • ヒト歯根膜由来細胞のRANKL, OPG mRNA発現に対するLPSの影響について

    和田尚久、前田英史、中牟田博敬、赤峰昭文

    第117回日本歯科保存学会秋季大会  2002.11 

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    Presentation type:Oral presentation (general)  

    Venue:徳島市   Country:Japan  

  • LPS stimulates OPG and RANKL expression in periodontal ligament. International conference

    Wada N, Maeda H, Akamine A

    82nd General Session & Exhibition of the IADR  2004.3 

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    Venue:Honolulu   Country:Taiwan, Province of China  

  • Fibroblastic cells from human periapical granulation tissue (HFC) preferentially form calcified matrices in decalcified and boiled rat bone. Invited International conference

    Maeda H, Wada N, Fujii S, Noda R, Akamine A

    World Conference on Dosing of Antiinfectives  2004.9 

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    Presentation type:Oral presentation (general)  

    Venue:Nurnberg   Country:Germany  

  • ヒト不死化歯根膜細胞株の樹立とキャラクタリゼーション

    藤井慎介、前田英史、和田尚久、野田亮、赤峰昭文

    第121回日本歯科保存学会秋季大会  2004.11 

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    Presentation type:Oral presentation (general)  

    Venue:長崎市   Country:Japan  

  • Establishment of human periodontal ligament cell lines and their characterization. International conference

    Fujii S, Maeda H, Wada N, Akamine A

    83rd General Session & Exhibition of the IADR  2005.3 

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    Venue:Baltimore   Country:United Arab Emirates  

  • Establishment of cell line of human epithelial rests of Malassez. International conference

    Wada N, Maeda H, Fujii S, Akamine A

    83rd General Session & Exhibition of the IADR  2005.3 

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    Venue:Baltimore   Country:United States  

  • ヒト不死化歯根膜細胞のクローニングとキャラクタリゼーション

    藤井慎介、前田英史、和田尚久、野田亮、赤峰昭文

    第122回日本歯科保存学会春季大会  2005.6 

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    Venue:札幌市   Country:Japan  

  • 九州大学病院口腔総合診療科における卒後教育環境評価への取り組み

    王丸寛美、松家洋子、住吉圭太、伊吹禎一、角義久、和田尚久、秋山陽一、樋口勝規

    日本歯科医学教育学会  2006.6 

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    Venue:仙台市   Country:Japan  

  • 研修歯科医への小矯正実習

    秋山陽一、住吉圭太、伊吹禎一、松家洋子、王丸寛美、角義久、和田尚久、樋口勝規

    日本歯科医学教育学会  2006.6 

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    Venue:仙台市   Country:Japan  

  • 九州大学病院歯科医師臨床研修における協力型施設と研修歯科医のマッチング

    松家洋子、王丸寛美、住吉圭太、伊吹禎一、和田尚久、角義久、秋山陽一、樋口勝規

    日本歯科医学教育学会  2006.6 

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    Venue:仙台市   Country:Japan  

  • MTA及びSuper-Bondのヒト歯根膜細胞の骨芽細胞様分化に及ぼす影響に関する研究

    野田亮、前田英史、藤井慎介、和田尚久、友清淳、吉嶺嘉人、赤峰昭文

    第27回日本歯内療法学会学術大会  2006.7 

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    Venue:徳島市   Country:Japan  

  • 高齢者の口腔機能低下症と心理社会的要因との関連

    萱野 綾華, 王丸 寛美, 和田 尚久

    日本歯科衛生学会雑誌  2023.8  日本歯科衛生学会

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  • 高齢入院患者に対し歯科摂食嚥下リハチームによる口腔健康管理によって食事内容の改善に寄与した症例

    井上 昂也, 山添 淳一, 荻野 洋一郎, 神野 哲平, 塚本 葉子, 萱野 綾華, 柏崎 晴彦, 竹下 徹, 和田 尚久

    口腔衛生学会雑誌  2024.7  (一社)日本口腔衛生学会

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  • 病棟ベッドからの転落により前歯補綴物と歯が脱落した入院患者に対して口腔健康管理を行った1例

    井上 昂也, 山添 淳一, 衛藤 希, 岐部 里子, 兼山 千波, 須賀 恵美, 柏崎 晴彦, 田中 洋輔, 和田 尚久

    日本口腔ケア学会雑誌  2023.4  (一社)日本口腔ケア学会

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  • 根管の状態に応じた歯内治療 根尖周囲組織への刺激や侵襲を臨床的にいかに防ぐか

    和田 尚久

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2023.5  (NPO)日本歯科保存学会

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  • 慢性疼痛患者における心身相関の可視化 PSGおよびウェアラブル筋電図24時間測定による咬筋筋活動測定の有用性

    津田 緩子, 細井 昌子, 中村 拓也, 田中 貫平, 村上 匡史, 吉田 博子, 坂本 英治, 田中 佑, 谷口 大吾, 藤本 晃嗣, 安野 広三, 和田 尚久, 須藤 信行

    臨床神経生理学  2023.10  (一社)日本臨床神経生理学会

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  • 当院における周術期食道癌患者に対する歯科摂食嚥下リハビリテーションチームの取り組み

    友岡 祥子, 井上 昂也, 山添 淳一, 荻野 洋一郎, 神野 哲平, 塚本 葉子, 萱野 綾華, 和田 尚久, 柏崎 晴彦

    日本口腔ケア学会雑誌  2024.4  (一社)日本口腔ケア学会

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  • 地域高齢住民における歯数とサルコペニア発症との関連 久山町研究

    友岡 祥子, 坂田 智子, 大石 絵美, 本田 貴紀, 秦 淳, 古田 美智子, 山下 喜久, 和田 尚久, 北園 孝成, 二宮 利治

    日本老年医学会雑誌  2023.10  (一社)日本老年医学会

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  • 全国の歯科患者における口腔の健康状態と身体的愁訴との縦断的な関連 8020推進財団 歯科医療による健康増進効果に関する研究

    井上 昂也, 古田 美智子, 須磨 紫乃, 深井 穫博, 嶋崎 義浩, 相田 潤, 安藤 雄一, 宮崎 秀夫, 神原 正樹, 和田 尚久, 山下 喜久

    口腔衛生学会雑誌  2022.4  (一社)日本口腔衛生学会

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  • 倫理・プロフェッショナリズム教育の現状 歯科医師臨床研修における倫理・プロフェッショナリズム教育の現状と課題

    和田 尚久

    日本歯科医学教育学会雑誌  2022.12  (一社)日本歯科医学教育学会

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  • スーパーMTAペーストはヒト前骨芽細胞の石灰化誘導能を促進する

    御手洗 裕美, Naati Fakatava, 王 恕心, 冉 子晴, 祐田 明香, 原口 晃, 孫 偉浩, 和田 尚久

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2023.5  (NPO)日本歯科保存学会

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  • IGFBP3は歯胚発生と歯周組織のリモデリングに関与する

    王 恕心, 御手洗 裕美, 冉 子晴, 祐田 明香, 孫 偉浩, 原口 晃, 前田 英史, 和田 尚久

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2023.10  (NPO)日本歯科保存学会

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  • ACTA2はTGF-β1の相互作用が有りと無しの機序を介してヒトPDL機能を調節する(ACTA2 regulates human PDL function via interaction with or without TGF-β1)

    Fakatava Naati, 御手洗 裕美, 祐田 明香, 原口 晃, 長谷川 大学, 前田 英史, 和田 尚久

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2022.5  (NPO)日本歯科保存学会

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MISC

  • 次世代の歯の治療-歯髄・根尖性歯周炎の再生-2 歯根膜再生における歯原性幹細胞とiPS細胞.

    和田 尚久, 前田 英史, 赤峰 昭文

    歯界展望 医歯薬出版社   2014.8

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  • Immunomodulatory Effects of Stem Cells

    Naohisa wada, Stan Gronthos, P.Mark Bartold

    Periodontol 2000. 63(1):198-216.   2013.10

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  • 臨床指針 食道癌手術症例における入院前周術期支援の意義

    原武直紀、清松丈浩、淀川千穂、須古井和美、伊藤心二、和田尚久、水元一博、中川尚志

    臨床と研究   2023.10

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  • 歯科医師臨床研修における倫理・プロフェッショナリズム教育の現状と課題.In:.倫理・プロフェッショナリズム教育の現状.

    平田創一郎、樫則章、二宮一智、長谷由紀子、和田尚久

    日本歯科医学教育学会雑誌   2022.10

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  • Periodontal Ligament Stem Cells Regenerative Potency in Periodontium

    Atsushi Tomokiyo, Naohisa Wada, Hidefumi Maeda

    Stem cells and development   2019.8

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    Periodontium is consisted of root cementum, bone lining the tooth socket, gingiva facing the tooth, and periodontal ligament (PDL). Its primary functions are support of the tooth and protection of tooth, nerve, and blood vessels from injury by mechanical loading. Severe periodontitis induces the destruction of periodontium and results in a significant cause of tooth loss among adults. Unfortunately, conventional therapies such as scaling and root planning are often only palliative. Therefore, the ultimate goal of the treatment for periodontitis is to restore disrupted periodontium to its original shape and function. Tissue engineering refers to the process of combining cells, scaffolds, and signaling molecules for the production of functional tissues to restore, maintain, and improve damaged organs. The discovery of periodontal ligament stem cells (PDLSCs) highlighted the possibility for development of tissue engineering technology-based therapeutics for disrupted periodontium. PDLSCs are a kind of somatic stem cells that show potential to differentiate into multiple cell types and undergo robust clonal self-renewal. Therefore, PDLSCs are considered a highly promising stem cell population for regenerative therapy in periodontium; however, their rarity prevents the progression of basic and clinical researches. In this review, we summarize recent research advancement and accumulated information regarding the self-renewal capacity, multipotency, and immunomodulatory effect of PDLSCs, as well as their contribution to repair and regeneration of periodontium and other tissues. We also discuss the possibility of PDLSCs for clinical application of regenerative medicine and provide an outline of the genetic approaches to overcome the issue about the rarity of PDLSCs.

    DOI: 10.1089/scd.2019.0031

  • Practical considerations for effective oral appliance use in the treatment of obstructive sleep apnea: a clinical review.

    Hiroko Tsuda, Naohisa Wada, S. Ando

    2017.6

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  • Immunomodulatory properties of PDLSC and relevance to periodontal regeneration.

    Naohisa Wada, Atsushi Tomokiyo, Stan Gronthos, P. Mark Bartold

    2015.8

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  • Is there a role for neural crest stem cells in periodontal regeneration?

    Atsushi Tomokiyo, Kim Hynes, Naohisa Wada, Stan Gronthos, P. Mark Bartold

    2015.8

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  • Regeneration of the periodontium for preservation of the damaged tooth.

    Hidefumi Maeda, Atsushi Tomokiyo, Naohisa Wada, Koori Katsuaki, G Kawachi, Akifumi Akamine

    2014.10

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  • Wnt5aはRor2-JNKシグナルを介してヒト歯根膜幹細胞株の骨芽細胞様分化を抑制する

    長谷川 大学, 和田 尚久, 前田 英史, 吉田 晋一郎, 御手洗 裕美, 門野内 聡, 濱野 さゆり, 祐田 明香, 赤峰 昭文

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   2014.10

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  • 次世代の歯の治療-歯髄・根尖性歯周炎の再生-2 歯根膜組織再生誘導を目指した治療法.

    前田 英史, 和田 尚久, 赤峰 昭文

    歯界展望 医歯薬出版社   2014.8

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  • Wnt5aはTGFβ1を介してヒト歯根膜細胞のコラーゲン線維形成を促進する

    長谷川 大学, 和田 尚久, 前田 英史, 吉田 晋一郎, 門野内 聡, 御手洗 裕美, 濱野 さゆり, 祐田 明香, 赤峰 昭文

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集   2014.6

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  • Periodontal Tissue Engineering: Defining the Triad

    Hidefumi Maeda, Fujii Shinsuke, Atsushi Tomokiyo, naohisa wada, Akifumi Akamine

    Int J Oral Maxillofac Implants. 28(6):e461-471.   2013.6

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  • Prospective Potency of TGF-β1 on Maintenance and Regeneration of Periodontal Tissue.

    Hidefumi Maeda, naohisa wada, Atsushi Tomokiyo, Monnouchi Satoshi, Akifumi Akamine

    Int Rev Cell Mol Biol. 304:283-367   2013.6

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    Language:English   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • Promise of periodontal ligament stem cells in regeneration of periodontium

    Maeda H, Tomokiyo A, Fujii S, Wada N, Akamine A

    Stem Cell Res Ther. 2:33.   2011.8

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  • レジンシーラーの細胞親和性について : スーパーボンド根充シーラーと AH Plus との比較

    前田 英史, 友清 淳, 郡 勝明, 藤井 慎介, 門野内 聡, 和田 尚久, 河野 清美, 山本 直秀, 寺松 陽子, 赤峰 昭文

    日本歯内療法学会雑誌 = The journal of Japan Endodontic Association   2011.5

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    Biocompatibility of resin-based sealers : Comparison of Superbond sealer with AH Plus

    DOI: 10.20817/jeajournal.32.2_97

  • Mineral trioxide aggregate(MTA)がヒト歯根膜線維芽細胞に及ぼす影響に関する研究

    前田 英史, 友清 淳, 藤井 慎介, 島 一也, 和田 尚久, 門野内 聡, 堀 清美, 中野 嗣久, 吉嶺 嘉人, 赤峰 昭文

    日本歯科保存学雑誌   2009.8

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    The Effects of Mineral Trioxide Aggregate (MTA) on Human Periodontal Ligament Cells
    Since mineral trioxide aggregate (MTA) was developed and reported as an endodontic restoration in the early 1990s, it has been shown that this material offers excellent biocompatibility with the hard tissue on its surface when used for direct pulp capping, furcal repair, repair for root perforation, root-end filling, and apexification, and for inducing tissue regeneration around the filled portion. However, the effects of MTA on the periodontal ligament tissue remain unclear, so this study evaluated the effects of MTA on the attachment, proliferation, and differentiation of human periodonta...

    DOI: 10.11471/shikahozon.52.355

  • MTAはヒト歯根膜細胞の骨芽細胞様分化を誘導する

    前田 英史, 中野 嗣久, 友清 淳, 藤井 慎介, 門野内 聡, 和田 尚久, 赤峰 昭文

    日本歯科保存学雑誌   2008.5

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    MTA induces osteoblastic differentiation of human periodontal ligament cells

    DOI: 10.1177/154405910808701002

  • 多分化能を有するヒト歯根膜クローン細胞株が神経細胞分化及び細胞遊走に及ぼす影響

    友清 淳, 前田 英史, 藤井 慎介, 和田 尚久, 門野内 聡, 赤峰 昭文

    日本歯科保存学雑誌   2008.5

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    A multipotent hPDL cell line promotes neurocytic differentiation and migration

  • EDTAならびにNaOClによる根管洗浄後のSEM観察 : 超音波洗浄との比較

    島 一也, 前田 英史, 後藤 康治, 畦森 雅子, 安田 善之, 和田 尚久, 藤井 慎介, 友清 淳, 吉嶺 嘉人, 齋藤 隆史, 赤峰 昭文

    日本歯内療法学会雑誌 = The journal of Japan Endodontic Association   2008.1

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    SEM images of root canal dentin irrigated with EDTA and NaOCl : Comparison with ultrasonic irrigation

    DOI: 10.20817/jeajournal.29.1_15

  • 九州大学病院歯科医師臨床研修における卒直後教育環境評価

    王丸 寛美, 角 義久, 伊吹 禎一, 松家 洋子, 住吉 圭太, 和田 尚久, 秋山 陽一, 樋口 勝規

    日本歯科医学教育学会雑誌 = Journal of Japanese Association for Dental Education   2007.10

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    Educational Environment Measure for the Residents in Postgraduate Clinical Training, Kyushu University Hospital

  • 九州大学病院歯科医師臨床研修における協力型施設と研修歯科医のマッチング

    松家 洋子, 王丸 寛美, 住吉 圭太, 伊吹 禎一, 和田 尚久, 角 義久, 秋山 陽一, 樋口 勝規

    日本歯科医学教育学会雑誌 = Journal of Japanese Association for Dental Education   2007.4

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    Matching between Dental Residents and External Facilities on a Postgraduate Clinical Training Program of Kyushu University Hospital

  • スメアクリーン洗浄後の根管象牙質のSEM観察像 : 超音波洗浄との比較

    島 一也, 前田 英史, 後藤 康治, 畦森 雅子, 和田 尚久, 藤井 慎介, 友清 淳, 赤峰 昭文

    日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY   2006.10

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    SEM images of root canal dentin irrigated with Smearclean : Comparison with ultrasonic irrigation

  • 多分化能を持つヒト歯根膜クローン細胞株の樹立とキャラクタリゼーション

    友清 淳, 前田 英史, 藤井 慎介, 和田 尚久, 島 一也, 赤峰 昭文

    日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY   2006.10

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    Establishment and Characterization of Human Periodontal Ligament Cell Lines with Multipotencies

  • MTAおよび Super-Bond のヒト歯根膜細胞の骨芽細胞様分化に及ぼす影響に関する研究

    野田 亮, 前田 英史, 藤井 慎介, 和田 尚久, 友清 淳, 吉嶺 嘉人, 赤峰 昭文

    日本歯内療法学会雑誌 = The journal of Japan Endodontic Association   2006.9

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    Effects of root-end filling materials on the osteoblast-like differentiation of human periodontal ligament cells

    DOI: 10.20817/jeajournal.27.3_126

  • ヒト不死化歯根膜クローン細胞におけるEMDおよびbFGFが石灰化誘導に及ぼす影響

    友清 淳, 前田 英史, 藤井 慎介, 和田 尚久, 野田 亮, 島 一也, 赤峰 昭文

    日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY   2005.10

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    Influence of EMD and bFGF on mineralization in human periodontal ligament cell lines

  • ヒト不死化歯根膜細胞株の樹立とキャラクタリゼーション

    藤井 慎介, 前田 英史, 和田 尚久, 野田 亮, 赤峰 昭文

    日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY   2004.10

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    Establishment of human periodontal ligament cell lines and their characterization

  • 根尖病変内の上皮組織におけるアポトーシスに関する組織学的研究

    和田 尚久, 前田 英史, 中牟田 博敬, 赤峰 昭文

    日本歯科保存学雑誌   2003.2

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    Apoptosis of Epithelial Cells Proliferated in Human Periapical Lesions

  • 10.歯髄炎の組織像 from Seltzer and Bender’s DENTAL PULP.

    赤峰昭文、吉嶺嘉人、橋口勇、前田英史、和田尚久

    Quintessence   2003.1

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    Language:Japanese  

  • ヒト歯根膜由来細胞のRANKL, OPG mRNA発現に対するLPSの影響について

    和田 尚久, 前田 英史, 中牟田 博敬, 赤峰 昭文

    日本歯科保存学雑誌   2002.10

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    The effects of LPS on RANKL and OPG mRNA expression of human periodontal ligament fibroblastic cells

  • U937-DE4細胞を用いたヒト破骨細胞様細胞形成系における転写因子Egr1の役割

    久木田 敏夫, 久木田 明子, 渡辺 敏之, 和田 尚久, 藤 加寿子, 飯島 忠彦

    歯科基礎医学会雑誌   2001.8

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  • 歯根膜細胞における破骨細胞形成抑制因子OPG/OCIFの発現

    和田 尚久, 前田 英史, 中牟田 博敬, 田邊 一成, 赤峰 昭文

    日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY   2000.3

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    The expression of osteoprotegerin (OPG)/osteoclastogenesis inhibitory factor (OCIF) in human periodontal ligament cells

  • Histological study of periapical tissue healing in the rat molar after retrofilling with various materials

    H Maeda, Hashiguchi, I, H Nakamuta, Y Toriya, N Wada, A Akamine

    JOURNAL OF ENDODONTICS   1999.1

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    We histologically examined the effects on the periapical tissue of various dental filling materials applied as retrofillings in rats and compared them with those of amalgam. The 4-META-TBB resin Superbond and the light-cured composite resin produced the least severe inflammatory reaction, with the greatest amount of new bone. In these specimens, regeneration of a part of the periodontal ligament was also observed. These results indicate that these materials might be very biocompatible and thus foster the natural regeneration of the periapical tissue.

  • 歯根肉芽腫由来の線維芽細胞様細胞は石灰化能を有する

    前田 英史, 中牟田 博敬, 和田 尚久, 赤峰 昭文

    日本歯科保存学雑誌   1998.10

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    Fibroblastic Cells Derived from Periapical Granuloma Have the Ability to Calcify

  • 水酸化カルシウム製材カルベックスの組織親和性に関する研究

    橋口 勇, 前田 英史, 和田 尚久, 中野 嗣久, 中牟田 博敬, 赤峰 昭文

    日本歯科保存学雑誌   1998.5

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    Histological examination on the biocompatibility of Calcipex.

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Professional Memberships

  • 日本総合歯科学会

  • 日本老年歯科学会

  • 日本顕微鏡歯科学会

  • 日本口腔ケア学会

  • Japan Asssociation of Dental Research

  • International Association for Dental Research

  • Japanese society of regenerative medicine

  • 日本歯科医学教育学会

  • 日本歯内療法学会

  • 日本歯科保存学会

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Committee Memberships

  • 日本歯科保存学会   Executive   Domestic

    2023.3 - 2025.3   

  • 日本顕微鏡歯科学会   Vice-chairman   Domestic

    2023.1 - 2024.12   

  • 日本歯科保存学会   Steering committee member   Domestic

    2021.4 - 2023.3   

  • 日本歯科保存学会   定款委員会   Domestic

    2021.4 - 2023.3   

  • 日本歯科保存学会   Executive   Domestic

    2021.3 - 2023.3   

  • 日本顕微鏡歯科学会   Executive   Domestic

    2021.1 - 2022.12   

  • 日本顕微鏡歯科学会   Steering committee member   Domestic

    2021.1 - 2022.12   

  • 日本顕微鏡歯科学会   運営委員会 委員   Domestic

    2021.1 - 2022.12   

  • 日本歯科医学教育学会   Steering committee member   Domestic

    2019.7 - 2021.3   

  • 日本歯科医学教育学会   Steering committee member   Domestic

    2019.7 - 2021.3   

  • 日本歯科医学教育学会   企画・将来構想委員会 副委員長   Domestic

    2019.7 - 2021.3   

  • 日本歯科医学教育学会   教育方略委員会   Domestic

    2019.7 - 2021.3   

  • 日本歯科保存学会   Steering committee member   Domestic

    2019.4 - 2023.3   

  • 日本歯科保存学会   学術用語委員会   Domestic

    2019.4 - 2023.3   

  • 日本総合歯科学会   Steering committee member   Domestic

    2019.4 - 2021.3   

  • 日本総合歯科学会   Steering committee member   Domestic

    2019.4 - 2021.3   

  • 日本総合歯科学会   渉外委員会委員長   Domestic

    2019.4 - 2021.3   

  • 日本総合歯科学会   認定制度委員会委員   Domestic

    2019.4 - 2021.3   

  • 日本歯科保存学会   Executive   Domestic

    2019.3 - 2021.3   

  • 日本顕微鏡歯科学会   Steering committee member   Domestic

    2019.1 - 2022.12   

  • 日本顕微鏡歯科学会   あり方委員会 委員   Domestic

    2019.1 - 2022.12   

  • 日本口腔ケア学会   Councilor   Domestic

    2018.4 - 2020.3   

  • 日本総合歯科学会   Steering committee member   Domestic

    2017.4 - 2019.3   

  • 日本総合歯科学会   認定制度委員会委員   Domestic

    2017.4 - 2019.3   

  • 日本顕微鏡歯科学会   Steering committee member   Domestic

    2017.1 - 2022.12   

  • 日本顕微鏡歯科学会   総務委員会 副委員長   Domestic

    2017.1 - 2022.12   

  • 日本顕微鏡歯科学会   Executive   Domestic

    2017.1 - 2018.12   

  • 日本歯科医学教育学会   Councilor   Domestic

    2016.4 - Present   

  • 日本総合歯科学会   Steering committee member   Domestic

    2015.11 - 2021.3   

  • 日本総合歯科学会   日本総合歯科学会表彰委員会委員   Domestic

    2015.11 - 2021.3   

  • 日本総合歯科学会   Executive   Domestic

    2015.11 - 2019.3   

  • 日本歯科医学教育学会   Steering committee member   Domestic

    2015.7 - 2019.4   

  • 日本歯科医学教育学会   倫理・プロフェッショナリズム教育委員会   Domestic

    2015.7 - 2019.4   

  • 日本歯科保存学会   Councilor   Domestic

    2013.4 - 2019.3   

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Academic Activities

  • 歯学系OSCE事後評価解析委員会/委員長

    Role(s): Review, evaluation

    医療系大学間共用試験実施評価機構  2024.4 - 2026.3

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    Type:Scientific advice/Review 

  • 座長

    第3回福岡口腔ケアフォーラム・日本口腔看護研究会第7回九州口腔ケアセミナー  ( Japan ) 2023.12

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    Type:Competition, symposium, etc. 

  • 座長

    第16回日本総合歯科学会総会・学術大会  ( Japan ) 2023.10

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    Type:Competition, symposium, etc. 

  • 歯学系OSCE事後評価解析委員会/副委員長

    Role(s): Review, evaluation

    医療系大学間共用試験実施評価機構  2023.4 - 2024.3

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    Type:Scientific advice/Review 

  • 副大会長、座長

    第23回日本口腔ケア協会学術大会、日本口腔ケア学会秋期大会  ( Japan ) 2022.11

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    Type:Competition, symposium, etc. 

  • 座長

    第15回日本総合歯科学会総会・学術大会  ( Japan ) 2022.11

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    Type:Competition, symposium, etc. 

  • 座長

    日本顕微鏡歯科学会第18 回学術大会・総会 大会長賞受賞講演  ( Japan ) 2022.4

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    Type:Competition, symposium, etc. 

  • OSCEのあり方・評価者養成に係る調査・実証事業推進会議 モデルOSCE検討WG/委員長

    Role(s): Review, evaluation

    医療系大学間共用試験実施評価機構  2022.4 - 2024.3

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    Type:Scientific advice/Review 

  • 大会長

    日本顕微鏡歯科学会第17回学術大会  ( WEB Japan ) 2021.4

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    Type:Competition, symposium, etc. 

  • OSCEのあり方・評価者養成に係る調査・実証事業推進会議委員

    Role(s): Review, evaluation

    医療系大学間共用試験実施評価機構  2021.4 - 2024.3

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    Type:Scientific advice/Review 

  • OSCEのあり方・評価者養成に係る調査・実証事業推進会議 資材・機材・顎模型等策定連携WG/委員長

    Role(s): Review, evaluation

    医療系大学間共用試験実施評価機構  2021.4 - 2022.3

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    Type:Scientific advice/Review 

  • 座長

    災害歯科口腔医療シンポジウム  ( WEB Japan ) 2020.8

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    Type:Competition, symposium, etc. 

  • 座長

    第12回日本総合歯科学会総会・学術大会  ( Japan ) 2019.11

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    Type:Competition, symposium, etc. 

  • 座長

    熊本大学病院災害医療教育研究センター第1回食支援ワークショップ  ( Japan ) 2019.8

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    Type:Competition, symposium, etc. 

  • 座長

    第38回日本歯科医学教育学会学術大会  ( Japan ) 2019.7

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    Type:Competition, symposium, etc. 

  • 副大会長

    第38回日本歯科医学教育学会学術大会  ( Japan ) 2019.7

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    Type:Competition, symposium, etc. 

  • 座長

    第6回日本医療連携研究会総会・研究集会  ( Japan ) 2019.7

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    Type:Competition, symposium, etc. 

  • 歯学系OSCE事後評価解析小委員会/副委員長

    Role(s): Review, evaluation

    医療系大学間共用試験実施評価機構  2019.4 - 2023.3

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    Type:Scientific advice/Review 

  • 歯学系OSCE実施小委員会・事後評価解析小委員会FD専門部会/委員

    Role(s): Review, evaluation

    医療系大学間共用試験実施評価機構  2019.4 - 2022.3

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    Type:Scientific advice/Review 

  • 優秀口演選考対象発表セッション1 座長

    第11回日本総合歯科学会総会・学術大会  ( Japan ) 2018.10

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    Type:Competition, symposium, etc. 

  • 副大会長

    第15回日本口腔ケア学会総会・学術大会  ( Japan ) 2018.4

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    Type:Competition, symposium, etc. 

  • 座長(Chairmanship)

    第15回日本口腔ケア学会総会・学術大会  ( Japan ) 2018.4

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    Type:Competition, symposium, etc. 

  • 歯学系OSCE事後評価解析小委員会

    Role(s): Review, evaluation

    医療系大学間共用試験実施評価機構  2017.4 - 2019.3

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    Type:Scientific advice/Review 

  • 歯学系OSCE実施小委員会・事後評価解析小委員会FD専門部会

    Role(s): Review, evaluation

    医療系大学間共用試験実施評価機構  2017.4 - 2018.3

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    Type:Scientific advice/Review 

  • 委員

    平成28年度第5回倫理・プロフェッショナリズムプロフェッショナリズム教育委員会  ( Japan ) 2017.1

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    Type:Competition, symposium, etc. 

    Number of participants:8

  • 座長(Chairmanship)

    第9回日本総合歯科学会総会・学術大会  ( Japan ) 2016.11

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    Type:Competition, symposium, etc. 

  • 研究分担者

    平成28年度長寿医療研究開発費 「近赤外光・レーザー等を用いた新たな歯科疾患診断・治療機器の開発に関する研究」 紫外線LED口腔治療装置の研究打合せ会議  ( Japan ) 2016.5

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    Type:Competition, symposium, etc. 

  • 出席者

    第2回 医療コミュニケーションガイド作成に関する会議  ( Japan ) 2016.3

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    Type:Competition, symposium, etc. 

    Number of participants:20

  • 座長(Chairmanship) International contribution

    頭脳循環第二回シンポジウム  ( Fukuoka Japan ) 2016.2

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    Type:Competition, symposium, etc. 

  • 研究協力者

    平成27年度長寿医療研究開発費 25-7「高齢者の口腔機能の維持・向上法に関する研究」班会議  ( Japan ) 2015.10

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Research Projects

  • Examination of oral rehabilitation methods that lead to increased appetite

    Grant number:24K22207  2024.6 - 2027.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Research (Exploratory)

    山添 淳一, 重村 憲徳, 和田 尚久

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    Grant type:Scientific research funding

    グレリンはヒトとラットの胃から同定されたペプチドで摂食亢進や体重増加、消化管機能調節などエネルギー代謝調節に重要な作用を持つことが知られている。本研究では次の点を明らかにしたい。①口腔機能と食欲およびグレリン分泌との関連性を解明し、②食欲増進およびグレリン分泌を促す口腔機能リハビリテーション法の検討をマウス実験およびヒトに応用した臨床研究にて行う。本研究から得られる結果により、口腔機能が低下した高齢者およびがん患者対象の新規口腔リハビリテーション戦略の構築に繋げていく。

    CiNii Research

  • Elucidation of differentiation regulating mechanism by SPARC-related factors expressed in the dental papilla, and attempt of tooth regeneration

    Grant number:23K09140  2023.4 - 2026.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    和田 裕子, 清島 保, 和田 尚久, 長谷川 佳那, 御手洗 裕美

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    これまでに上皮系幹細胞と間葉系幹細胞によって歯の再生を促す方法が提案されているが実現には至っていない。我々は、先行研究にて微量RNA seq解析により、歯胚発生過程の上皮間葉相互作用における歯乳頭に高発現する因子を同定した。本研究では、歯乳頭に発現するSPARC関連因子による歯髄血管新生制御機構および象牙芽細胞の分化制御機構を解明し、歯の形態形成への影響を明らかにすることを目的とする。本研究で得られた結果により、発生・再生研究や幹細胞研究において有益な情報を提供し、歯の再生治療を含めた新規治療法の開発を目指す。

    CiNii Research

  • ICU患者におけるせん妄発症に関連した口腔状態の危険因子の解明

    Grant number:23K09506  2023.4 - 2026.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    神野 哲平, 赤星 朋比古, 和田 尚久

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    Grant type:Scientific research funding

    せん妄は ICU 患者の予後不良因子の 1つとして知られている。ICUでのせん妄発症は、ICU在室日数および入院期間の延長、医療費の増大、死亡率の上昇、ICU 退室後も続く認知機能の低下などに関連するため、患者のせん妄発症リスクから発症予測を行うことは、重要な視点となりうる。本研究では、せん妄発症に関連する口腔状態の危険因子を解明する。予備研究において、舌細菌数がせん妄の発症予測因子であることが示唆されており、口腔細菌叢とせん妄との関連性についても解析を行う。ICU患者の早期回復と退院後のQOLの向上へ、口腔から始める新たなせん妄対策を提案することを目指す。

    CiNii Research

  • 歯学教育及び歯科医師臨床研修において一貫して利用できるオンライン 評価システムの開発に関する研究

    Grant number:22AC1001  2023

    Grants-in-Aid for Scientific Research  Grants-in-Aid for Scientific Research (Ministry of Health, Labour and Welfare)

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    Authorship:Coinvestigator(s)  Grant type:Competitive funding other than Grants-in-Aid for Scientific Research

  • 歯内治療関連研究費

    2023

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    Grant type:Donation

  • 歯周組織発生過程の模倣を基盤とするハイブリッド構造体の創成と歯周組織再生への応用

    Grant number:23K24529  2022.4 - 2026.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    友清 淳, 前田 英史, 糸山 知宏, 和田 尚久, 小幡 純子, 杉井 英樹, 藤野 翔香

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    Grant type:Scientific research funding

    歯周組織は神経堤細胞 (NC) に発生を由来することから、NCは歯周組織の発生過程を模倣する上で極めて重要な細胞であると考えられる。申請者らは人工多能性幹細胞 (iPSC) からNC様細胞 (iPSC-NC) への分化誘導法、およびiPSC-NCから歯根膜幹細胞様細胞 (iPSC-PDLSC) への分化誘導法を確立している。そこで本研究では、これらの細胞に対するゲノム解析結果から同定した、iPSC-NCからiPSC-PDLSCへの分化を誘導する形態形成因子および足場材について至適化を行い、その結果を基にiPSC-NC/形態形成因子/足場材から構成されるハイブリッド構造体を作製する。

    CiNii Research

  • 歯学教育及び歯科医師臨床研修において一貫して利用できるオンライン 評価システムの開発に関する研究

    2022.4 - 2024.3

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    Authorship:Coinvestigator(s) 

  • 歯周組織発生過程の模倣を基盤とするハイブリッド構造体の創成と歯周組織再生への応用

    Grant number:22H03271  2022 - 2025

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 舌苔細菌叢による難治性悪性腫瘍早期発見の可能性についての研究

    Grant number:22K10364  2022 - 2024

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    稲井 裕子, 和田 尚久

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    臨床的に悪性度が高く発見することが困難なスキルス胃癌、診断が確定した時にはすでに進行したステージで、予後不良である膵臓癌などの症例に遭遇することも多く、早期発見の必要性を強く認識してきた。今回、我々は難治性悪性腫瘍早期発見のための検査の一つとして舌苔細菌叢の検査が有効ではないかと考え、症例の舌苔細菌叢を分析し、難治性悪性腫瘍の早期発見のための検査としての位置づけおよびその可能性について検討する

    CiNii Research

  • 根尖病巣複合Biofilmの性状解明と天然成分エッセンシャルオイルの有用性

    Grant number:22K10002  2022 - 2024

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    原口 晃, 和田 尚久

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

    根尖性歯周炎は細菌感染で惹起される感染症であり、心疾患との関連も報告されている。E. faecalisは、根管治療中に高頻度に検出され、菌血症や感染性心内膜炎との関連も示唆され、根管内に侵入し他種細菌とBiofilmを形成し治療に抵抗性を獲得するとされる。根管内の感染源の除去には革新的治療法の開発が望まれる。天然に存在するTerpinen-4-olは抗菌性をはじめ多くの生物学的活性を有する。ここでは根尖性歯周炎において菌同士の相互作用を解明し、複合Biofilmに対してTerinen-4-olを用いた新規抑制方法を検討する。これらは治療の成功率の向上及び全身への波及を阻止することを目的とする。

    CiNii Research

  • Delivery of a therapeutic protein for periodontal regeneration from grid sheets

    Grant number:22K09985  2022 - 2024

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    祐田 明香, 濱野 さゆり, 和田 尚久

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

    酸化グラフェンは、近年、生体応用への足掛かりが報告されている。Zhangらはコラーゲン-酸化グラフェン複合体を作製し研究を行った結果、間葉系幹細胞の骨芽細胞分化が促進されたことを報告している。また、格子状のシートの使用は、格子サイズにより細胞の成長に違いがあることを見いだされためである。さらに、ゼラチンハイドロゲルは、様々な生体組織の再生の修復実現に使用されている。例えば、FGF-2の徐放技術は、骨、軟膏の再生治療、虚血性心疾患に対する血管誘導治療を可能としている。以上のことから、細胞成長因子デリバリー格子状シートの新規歯周組織再生療法への応用を検討することとした。

    CiNii Research

  • 歯学教育及び歯科医師臨床研修において一貫して利用できるオンライン 評価システムの開発に関する研究

    Grant number:22AC0101  2022

    Grants-in-Aid for Scientific Research  Grants-in-Aid for Scientific Research (Ministry of Health, Labour and Welfare)

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    Authorship:Coinvestigator(s)  Grant type:Competitive funding other than Grants-in-Aid for Scientific Research

  • 歯内治療関連研究費

    2022

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    Grant type:Donation

  • Development of a new drug for periodontal regeneration based on the activation of neural crest cells

    Grant number:21K19608  2021.7 - 2024.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Research (Exploratory)

    友清 淳, 前田 英史, 和田 尚久, 杉井 英樹, 吉田 晋一郎, 小幡 純子, 糸山 知宏, 小野 太雅

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    Grant type:Scientific research funding

    歯周病は、歯を支持する歯周組織を破壊することで歯の動揺や疼痛を引き起こし、重度の
    場合には抜歯が必要となることもある。その一方で、破壊された歯周組織を再生させる決定
    的な治療法は、未だ確立されていない。また歯周病は、世界的に極めて高い罹患率を示す疾
    患であることから、世界規模で歯周病患者の歯周組織再生を実現するためには、発展途上国
    のような、歯科医療設備が十分整っていない環境下でも実施可能な治療法を開発する必要がある。本研究では、iPS細胞の遺伝子情報から得られた情報を基に、歯根膜に存在する神経堤細胞を賦活化させ、歯周組織の再生を促進する革新的歯周組織再生薬を創出することを目的とする。

    CiNii Research

  • 歯内治療関連研究費

    2021

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    Grant type:Donation

  • 新型コロナウイルス感染症拡大下における歯科医師臨床研修の継続及び適切な実施に向けた情報通信機器活用法の調査研究

    Grant number:20CA2072  2020

    Grants-in-Aid for Scientific Research  Grants-in-Aid for Scientific Research (Ministry of Health, Labour and Welfare)

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    Authorship:Coinvestigator(s)  Grant type:Competitive funding other than Grants-in-Aid for Scientific Research

  • 歯内治療関連研究費

    2020

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    Grant type:Donation

  • オミックス解析を用いた歯根膜発生機構の解明と幹細胞誘導型組織再生技術への応用

    Grant number:19H03832  2019 - 2022

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    和田 尚久, 前田 英史, 友清 淳, 祐田 明香, 藤井 慎介, 御手洗 裕美, 和田 裕子

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    Authorship:Principal investigator  Grant type:Scientific research funding

    近年、歯周病などによって大きく喪失した歯周組織の新たな再生療法として、幹細胞移植によって歯根膜および歯槽骨の再生を積極的に促す方法が提案されているが実現に至っていない。本研究では、歯胚発生期に着目してオミックス解析により歯根膜発生・形成に重要な特異的因子を同定、機能解析することで歯根膜発生機構の一端を解明する。さらに歯根膜発生・形成に重要な同定因子を幹細胞分化制御候補因子として歯根膜細胞への分化誘導能についてin vitroおよびin vivo両面から機能解析を行い、歯根膜組織再生のための幹細胞分化制御機構を解明する。

    CiNii Research

  • Research on the role and function of zinc transporters in oral squamous cell carcinoma

    Grant number:19K10313  2019 - 2021

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Jinno Teppei

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    ZIP6 is a zinc transporter regulated by inflammatory cytokines, and ZIP6 in particular has been shown to be an essential factor for EMT, and is a molecule that is attracting attention for its relationship with cancer growth, invasion, and metastasis. ... In fact, it has been reported that breast cancer cells in which ZIP6 expression is suppressed have their proliferation ability and EMT suppressed, so it is expected that detailed analysis of the expression of these zinc transporters will progress. . In this study, we investigated the expression of zinc transporters in OSCC cells, and investigated the effects of zinc transporters on anticancer drug resistance in OSCC cells and their relationship with IL-6 signaling.

    CiNii Research

  • 長寿医療研究開発費 レーザー光・紫外線LED等を用いた新たな歯科疾患診断・治療機器の開発に関する研究

    2018.4 - 2019.3

    Joint research

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    Authorship:Coinvestigator(s)  Grant type:Other funds from industry-academia collaboration

  • iPS創薬とゲノム創薬の融合による歯根膜幹細胞賦活化物質の創出と再生医療への応用

    Grant number:18K19651  2018 - 2020

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Challenging Research(Exploratory)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 長寿医療研究開発費 レーザー光・紫外線LED等を用いた新たな歯科疾患診断・治療機器の開発に関する研究

    2017.4 - 2018.3

    Joint research

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    Authorship:Coinvestigator(s)  Grant type:Other funds from industry-academia collaboration

  • iPS細胞を用いた3次元的歯根膜作製法の確立と人工歯根開発への応用

    Grant number:17H01598  2017 - 2022

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

    前田 英史, 友清 淳, 吉田 晋一郎, 濱野 さゆり, 和田 尚久, 杉井 英樹, 長谷川 大学

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    iPS細胞から歯根膜幹細胞様細胞(iPDLSC)への分化誘導法を明らかにし、分化に関わる因子として、転写因子PAX9およびFibrillin 2を検出した。また、ヒト歯根膜幹細胞株から作製したスフェロイドを、バイオ3Dプリンターを用いてチューブ状の構造体を作製した。この構造体は、ハイドロキシアパタイト(HAp)棒を内部に挿入することで、歯根膜様の特性の獲得に加えてセメント質、血管および骨の特性を示すことを確認した。そこで、iPDLSCのスフェロイドを用いて同様にチューブ状構造体を作製し、HAp棒を挿入して生体内に移植を行い、骨への生着を確認した。

    CiNii Research

  • iPS由来神経堤細胞によるバイオミメティクスに基づいた新規歯周組織再生療法の開発

    Grant number:17H04385  2017 - 2021

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 癌治療患者に発症する味覚異常のメカニズム解明と治療法の開発

    Grant number:17K12050  2017 - 2020

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 歯周病原菌が消化器癌に及ぼす影響に関する多面的研究

    Grant number:17K12035  2017 - 2019

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 広範囲の欠損修復を可能とする生体親和性良好な新規直接覆髄剤料の開発

    Grant number:17K17136  2017 - 2018

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 長寿医療研究開発費 近赤外光・レーザー等を用いた新たな歯科疾患診断・治療機器の開発に関する研究

    2016.4 - 2017.3

    Joint research

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    Authorship:Coinvestigator(s)  Grant type:Other funds from industry-academia collaboration

  • 近赤外光・レーザー等を用いた新たな歯科疾患診断・治療機器の開発に関する研究

    2016.4 - 2016.3

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    Authorship:Coinvestigator(s) 

  • 歯根膜幹細胞誘導による組織再生を基盤とした包括的歯内疾患治療法の開発

    Grant number:15H05023  2015 - 2018

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 研究支援

    2015

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    Grant type:Donation

  • セマフォリン3Aと歯髄幹細胞を用いた新規象牙質/歯髄複合体再生直接覆髄法の開発

    Grant number:26670826  2014 - 2016

    Grants-in-Aid for Scientific Research  Grant-in-Aid for challenging Exploratory Research

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • カルシウム感知受容体制御に着目した象牙質修復治療法の開発

    Grant number:10284514  2014 - 2016

    Grants-in-Aid for Scientific Research  Grant-in-Aid for challenging Exploratory Research

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 新規覆髄材の開発を目的としたβig-h3の解析

    Grant number:26462887  2014

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 歯根膜組織形成能を有したバイオアクティブレジンの開発

    Grant number:25293388  2013 - 2016

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 歯肉線維芽細胞由来iPS細胞を用いた新たな歯根膜組織誘導ストラテジー

    Grant number:25670811  2013 - 2014

    Grants-in-Aid for Scientific Research  Grant-in-Aid for challenging Exploratory Research

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 歯根膜細胞由来のiPS細胞の作製と歯根膜組織再生法の開発

    Grant number:24390426  2012 - 2014

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 歯髄幹細胞は歯牙再植時の歯周組織再生の細胞源となりうるか?

    Grant number:24659848  2012 - 2013

    Grants-in-Aid for Scientific Research  Grant-in-Aid for challenging Exploratory Research

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 新規の歯根膜幹細胞表面抗原および歯根膜マーカーを活用した歯周組織再生法の開発

    Grant number:23689077  2011 - 2013

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (A)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • アンギオテンシンIIを用いた成功率の高い意図的歯牙再植術の開発

    Grant number:23659890  2011 - 2012

    Grants-in-Aid for Scientific Research  Grant-in-Aid for challenging Exploratory Research

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • Clinical application of periodontal ligament stem cells to regenerate the periodontal tissue International coauthorship

    2010.4

    Colgate Australian Clinical Dental Research Centre University of Adelaide Department of Dentistry 

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    歯根膜幹細胞を応用した新たな歯周組織再生法を確立する。

  • 歯根膜を有した次世代型人工歯根の開発

    Grant number:22390359  2010 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 歯周靭帯の再生を主軸にした新規歯周組織再生療法の開発

    Grant number:22592123  2010 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 歯根膜幹細胞に特異的に発現する新規細胞表面抗原マーカーおよび増殖因子の同定

    Grant number:22890135  2010 - 2011

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (Start-up)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • Identification and characterisation of novel cell surface markers that are essential for periodontal ligament stem cell phenotype

    2010

    Australian Dental Research Foundation, Research Grant 2010

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    Authorship:Principal investigator  Grant type:Contract research

  • 歯根膜はその再生に何を必要とするか?

    Grant number:21390510  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • Immunosuppressive mechanisms of allogenic periodontal ligament stem cells on T-lymphocytes

    2009

    Australian Dental Research Foundation, Research Grant 2009

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    Authorship:Principal investigator  Grant type:Contract research

  • Reprogramming of human gingival and periodontal fibroblasts to pluripotency with defined factors

    2008

    The University of Adelaide, School of Dentistry, INTERNAL RESEARCH FUNDING 2008

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    Authorship:Principal investigator  Grant type:Contract research

  • ヒト歯根膜前駆細胞クローン細胞株を用いた歯根膜組織再生機構の解明

    Grant number:19390486  2007 - 2009

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • in vivo実験系を用いた歯周組織再生を促進する因子の同定

    Grant number:19592206  2007 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 歯周組織再生の鍵をにぎる細胞とその必須の因子は何か?

    Grant number:18390506  2006 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

  • 歯科治療におけるコンポジットレジン修復の臨床成績に及ぼす諸因子の影響

    2006 - 2007

    第2回ふくおか「臨床医学研究賞」

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    Authorship:Coinvestigator(s)  Grant type:Contract research

  • マラッセの上皮遺残はセメント質形成に関与しているか?

    Grant number:17791359  2005 - 2006

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 歯根膜における破骨細胞形成抑制因子OPG/OCIFの発現および調節機構の解析

    Grant number:10119  2001 - 2002

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for JSPS Fellows

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    Authorship:Principal investigator  Grant type:Scientific research funding

▼display all

Educational Activities

  • My educational activities are supervision and direction of Residents and students of 5th and 6th grade. Especially, general dental treatments, operative dentistry and endodontology are my specialty to teach young doctors.

Class subject

  • 医療行動科学3

    2024.12 - 2025.2   Winter quarter

  • 行動科学2

    2024.10 - 2025.3   Second semester

  • 医療行動科学2

    2024.10 - 2024.12   Fall quarter

  • アドバンスト臨床実習

    2024.6 - 2024.8   Summer quarter

  • 医療行動科学1

    2024.4 - 2024.9   First semester

  • 行動科学1

    2024.4 - 2024.9   First semester

  • Interdisciplinary Dentistry (Lower-grade) D

    2023.12 - 2024.2   Winter quarter

  • Interdisciplinary Dentistry (Core) D

    2023.12 - 2024.2   Winter quarter

  • 行動科学2

    2023.10 - 2024.3   Second semester

  • Interdisciplinary Dentistry (Lower-grade) C

    2023.10 - 2023.12   Fall quarter

  • Interdisciplinary Dentistry (Core) C

    2023.10 - 2023.12   Fall quarter

  • アドバンスト臨床実習

    2023.6 - 2023.8   Summer quarter

  • Interdisciplinary Dentistry (Core) B

    2023.6 - 2023.8   Summer quarter

  • Interdisciplinary Dentistry (Lower-grade) B

    2023.6 - 2023.8   Summer quarter

  • 総合歯科学臨床実習Ⅳ

    2023.4 - 2024.3   Full year

  • Clinical PracticeⅠ(Interdisciplinary Dentistry)

    2023.4 - 2024.3   Full year

  • Clinical PracticeⅡ(Interdisciplinary Dentistry)

    2023.4 - 2024.3   Full year

  • 総合歯科学臨床実習Ⅱ

    2023.4 - 2024.3   Full year

  • 行動科学1

    2023.4 - 2023.9   First semester

  • Interdisciplinary Dentistry (Lower-grade) A

    2023.4 - 2023.6   Spring quarter

  • Interdisciplinary Dentistry (Core) A

    2023.4 - 2023.6   Spring quarter

  • Interdisciplinary Dentistry (Lower-grade) D

    2022.12 - 2023.2   Winter quarter

  • 総合歯科学(低年次) D

    2022.12 - 2023.2   Winter quarter

  • 行動科学2

    2022.10 - 2023.3   Second semester

  • Interdisciplinary Dentistry (Lower-grade) C

    2022.10 - 2022.12   Fall quarter

  • 総合歯科学(低年次) C

    2022.10 - 2022.12   Fall quarter

  • Interdisciplinary Dentistry (Lower-grade) B

    2022.6 - 2022.8   Summer quarter

  • アドバンスト臨床実習

    2022.6 - 2022.8   Summer quarter

  • 総合歯科学(低年次) B

    2022.6 - 2022.8   Summer quarter

  • 総合歯科学臨床実習Ⅱ

    2022.4 - 2023.3   Full year

  • 総合歯科学特論

    2022.4 - 2023.3   Full year

  • 総合歯科学臨床実習Ⅲ

    2022.4 - 2023.3   Full year

  • 総合歯科学臨床実習Ⅰ

    2022.4 - 2023.3   Full year

  • 総合歯科学特論

    2022.4 - 2023.3   Full year

  • 行動科学1

    2022.4 - 2022.9   First semester

  • Interdisciplinary Dentistry (Lower-grade) A

    2022.4 - 2022.6   Spring quarter

  • 総合歯科学(低年次) A

    2022.4 - 2022.6   Spring quarter

  • 行動科学2

    2021.10 - 2022.3   Second semester

  • アドバンスト臨床実習

    2021.6 - 2021.8   Summer quarter

  • 総合歯科学(高年次)A-D

    2021.4 - 2022.3   Full year

  • 総合歯科学(低年次)A-D

    2021.4 - 2022.3   Full year

  • 総合歯科学(コア)A-D

    2021.4 - 2022.3   Full year

  • 総合歯科学特論

    2021.4 - 2022.3   Full year

  • 総合歯科学臨床実習Ⅱ

    2021.4 - 2022.3   Full year

  • 歯科臨床実習

    2021.4 - 2022.3   Full year

  • 総合歯科学臨床実習Ⅰ‐Ⅳ

    2021.4 - 2022.3   Full year

  • 行動科学1

    2021.4 - 2021.9   First semester

  • 行動科学2

    2020.10 - 2021.3   Second semester

  • アドバンスト臨床実習

    2020.6 - 2020.8   Summer quarter

  • 総合歯科学特論

    2020.4 - 2021.3   Full year

  • 統合歯科学特論(総合歯科学)

    2020.4 - 2021.3   Full year

  • 統合歯科学特別研究(総合歯科学)

    2020.4 - 2021.3   Full year

  • 総合歯科学研究入門

    2020.4 - 2021.3   Full year

  • 危機管理学

    2020.4 - 2021.3   Full year

  • 歯科医療行動学演習

    2020.4 - 2021.3   Full year

  • 総合歯科学臨床実習Ⅰ

    2020.4 - 2021.3   Full year

  • Interdisciplinary Dentistry

    2020.4 - 2021.3   Full year

  • 臨床基礎演習(総合歯科学)

    2020.4 - 2021.3   Full year

  • 総合歯科学臨床実習Ⅲ

    2020.4 - 2021.3   Full year

  • 総合歯科学臨床実習Ⅱ

    2020.4 - 2021.3   Full year

  • 行動科学1

    2020.4 - 2020.9   First semester

  • 行動科学2

    2019.10 - 2020.3   Second semester

  • アドバンスト臨床実習

    2019.6 - 2019.8   Summer quarter

  • 総合歯科学臨床実習Ⅲ

    2019.4 - 2020.3   Full year

  • 統合歯科学特論(総合歯科学)

    2019.4 - 2020.3   Full year

  • 臨床基礎演習(総合歯科学)

    2019.4 - 2020.3   Full year

  • 統合歯科学特別研究(総合歯科学)

    2019.4 - 2020.3   Full year

  • 総合歯科学研究入門

    2019.4 - 2020.3   Full year

  • 危機管理学

    2019.4 - 2020.3   Full year

  • 歯科医療行動学演習

    2019.4 - 2020.3   Full year

  • 総合歯科学臨床実習Ⅰ

    2019.4 - 2020.3   Full year

  • 総合歯科学特論

    2019.4 - 2020.3   Full year

  • Integrated Dental Science(総合歯科学)

    2019.4 - 2020.3   Full year

  • Introduction to Oral Biological Research

    2019.4 - 2020.3   Full year

  • Interdisciplinary Dentistry

    2019.4 - 2020.3   Full year

  • Clinical PracticeⅠ(総合歯科学)

    2019.4 - 2020.3   Full year

  • 総合歯科学臨床実習Ⅱ

    2019.4 - 2020.3   Full year

  • 行動科学1

    2019.4 - 2019.9   First semester

  • 行動科学2

    2018.10 - 2019.3   Second semester

  • 臨床基礎演習 (総合歯科学)

    2018.4 - 2019.3   Full year

  • 臨床実習

    2018.4 - 2019.3   Full year

  • 危機管理学

    2018.4 - 2019.3   Full year

  • 歯科医療行動学演習

    2018.4 - 2019.3   Full year

  • 総合歯科学臨床実習Ⅰ

    2018.4 - 2019.3   Full year

  • 統合歯科学特別研究 (総合歯科学)

    2018.4 - 2019.3   Full year

  • 総合歯科学研究入門

    2018.4 - 2018.9   First semester

  • 臨床予備実習

    2018.4 - 2018.9   First semester

  • 行動科学1

    2018.4 - 2018.9   First semester

  • 臨床実習

    2017.4 - 2018.3   Full year

  • 臨床基礎演習 (総合歯科学)

    2017.4 - 2018.3   Full year

  • 統合歯科学特別研究 (総合歯科学)

    2017.4 - 2018.3   Full year

  • 総合歯科学臨床実習Ⅰ

    2017.4 - 2018.3   Full year

  • 歯科医療行動学演習

    2017.4 - 2018.3   Full year

  • 危機管理学

    2017.4 - 2018.3   Full year

  • 臨床予備実習

    2017.4 - 2017.9   First semester

  • 行動科学Ⅰ

    2017.4 - 2017.9   First semester

  • 総合歯科学研究入門

    2017.4 - 2017.9   First semester

  • 臨床予備見学実習

    2017.4 - 2017.9   First semester

  • 歯科保存学Ⅰ

    2016.10 - 2017.3   Second semester

  • 総合歯科学

    2016.10 - 2017.3   Second semester

  • 臨床実習

    2016.4 - 2017.3   Full year

  • 臨床基礎演習 (総合歯科学)

    2016.4 - 2017.3   Full year

  • 統合歯科学特別研究 (総合歯科学)

    2016.4 - 2017.3   Full year

  • 総合歯科学臨床実習Ⅰ

    2016.4 - 2017.3   Full year

  • 歯科医療行動学演習

    2016.4 - 2017.3   Full year

  • 危機管理学

    2016.4 - 2017.3   Full year

  • 臨床予備実習

    2016.4 - 2016.9   First semester

  • 行動科学Ⅱ

    2016.4 - 2016.9   First semester

  • 総合歯科学研究入門

    2016.4 - 2016.9   First semester

  • 臨床予備見学実習

    2016.4 - 2016.9   First semester

  • 行動科学Ⅰ

    2015.10 - 2016.3   Second semester

  • 統合歯科学特別研究 (総合歯科学)

    2015.10 - 2016.3   Second semester

  • 歯科医療行動学演習

    2015.10 - 2016.3   Second semester

  • 危機管理学

    2015.10 - 2016.3   Second semester

  • 歯科保存学Ⅰ

    2015.10 - 2016.3   Second semester

  • 臨床実習

    2015.4 - 2016.3   Full year

  • 臨床予備見学実習

    2015.4 - 2015.9   First semester

  • 臨床予備実習

    2015.4 - 2015.9   First semester

  • 行動科学Ⅱ

    2015.4 - 2015.9   First semester

  • 総合歯科学

    2014.10 - 2015.3   Second semester

  • 第5、6学年臨床実習

    2014.10 - 2015.3   Second semester

  • 第5学年臨床予備実習

    2014.10 - 2015.3   Second semester

  • 歯科保存学

    2014.10 - 2015.3   Second semester

  • 臨床予備見学実習

    2014.4 - 2015.3   Full year

  • 行動科学II

    2014.4 - 2014.9   First semester

  • 歯科保存学

    2013.10 - 2014.3   Second semester

  • 第5学年臨床予備実習

    2013.10 - 2014.3   Second semester

  • 総合歯科学

    2013.10 - 2014.3   Second semester

  • 歯科保存学

    2012.10 - 2013.3   Second semester

  • 第5学年臨床予備実習

    2012.10 - 2013.3   Second semester

  • 総合歯科学

    2012.10 - 2013.3   Second semester

  • 行動科学II

    2012.4 - 2012.9   First semester

  • 生物科学II

    2012.4 - 2012.9   First semester

  • 第5学年臨床予備実習

    2011.10 - 2012.3   Second semester

  • 総合歯科学

    2011.10 - 2012.3   Second semester

  • 歯科保存学

    2011.10 - 2012.3   Second semester

  • 第5学年臨床予備実習

    2010.10 - 2011.3   Second semester

  • 総合歯科学

    2010.10 - 2011.3   Second semester

▼display all

FD Participation

  • 2023.6   Role:Participation   Title:科学研究費補助金採択率向上に向けた工夫等について

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2020.6   Role:Participation   Title:電子ジャーナルをめぐる現状と今後に向けた対応について

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2019.10   Role:Participation   Title:馬出地区4部局合同男女共同参画FD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2019.9   Role:Participation   Title:FD 科学研究費採択に向けて

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2019.5   Role:Participation   Title:一斉技能試験トライアル教員FD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2019.3   Role:Participation   Title:2019年度臨床実地試験トライアル説明会(FD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2018.10   Role:Participation   Title:平成30年度馬出地区4部局合同男女共同参画FD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2018.5   Role:Participation   Title:臨床実習後臨床能力試験トライアルの実施に向けて

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2018.3   Role:Participation   Title:東京医歯大における国家試験対策の実践

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2017.11   Role:Participation   Title:ARO次世代医療

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2016.12   Role:Participation   Title:講演 「歯学教育の現状と課題」「反グローバル化と国際交流」

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2016.5   Role:Participation   Title:CBT FD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2015.9   Role:Participation   Title:歯学教育に関するFD(文部科学省講師、新潟大学講師)

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2015.1   Role:Participation   Title:ルーブリック評価スタートアップ

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2014.5   Role:Participation   Title:平成26年度第1回共用試験歯学系OSCE評価者養成ワークショップII

  • 2014.1   Role:Participation   Title:平成25年度全人的医療人育成教育プログラム(臨床指導者コース)ワークショップ

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2013.3   Role:Participation   Title:チーム基盤型学習法(Team-Based Learning, TBL)

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2012.9   Role:Participation   Title:「英語による教授能力」向上のためのワークショップ

    Organizer:University-wide

  • 2012.1   Role:Participation   Title:日本人歯科医師の海外における活動 ー現状と可能性―

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2011.9   Role:Participation   Title:PBLチュートリアル教育 チューター講習会

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2011.8   Role:Participation   Title:平成23年度 九州大学歯学部カリキュラムプランニング講習会

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2011.1   Role:Participation   Title:昭和大学におけるPBL-チュートリアルの取り組み

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2011.1   Role:Participation   Title:歯学教育を取り巻く環境変化と求められる対応 =歯学教育の改善・充 実に関する調査研究協力者会議を中心に=

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2011.1   Role:Participation   Title:歯科保健医療対策の動向

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2010.12   Role:Participation   Title:カリキュラムプランニングの基礎 ―目標・方略・評価―

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2010.12   Role:Participation   Title:学び方を学ぶPBLチュートリアル ―高知大学での実践にもとづいて

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2010.4   Role:Participation   Title:平成22年度第1回全学FD

    Organizer:University-wide

  • 2007.4   Role:Participation   Title:平成19年度第1回FD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2006.7   Role:Participation   Title:平成18年度第1回FD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2006.6   Role:Participation   Title:平成18年度九州大学病院・歯科医師臨床研修指導歯科医講習会(厚生労働省・財団法人歯科医療研修振興財団共済)

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2006.3   Role:Participation   Title:平成17年度第1回FD

    Organizer:[Undergraduate school/graduate school/graduate faculty]

▼display all

Visiting, concurrent, or part-time lecturers at other universities, institutions, etc.

  • 2023  福岡歯科大学  Classification:Part-time lecturer  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:9/6

  • 2023  福岡国際医療福祉大学看護学科  Classification:Part-time lecturer  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:2023. 12/25

  • 2023  令和5年度医療法人社団湧泉会ひまわり歯科歯科医師臨床研修指導歯科医講習会 タスクフォース  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:令和5年9月17、18日

  • 2023  第26回中国・四国地区歯科医師臨床研修指導歯科医講習会 タスクフォース  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:令和6年2月10,11日

  • 2022  2022年度医療法人社団湧泉会ひまわり歯科歯科医師臨床研修指導歯科医講習会 タスクフォース  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:令和4年6月25、26日

  • 2022  令和4年度福岡歯科大学医科歯科総合病院指導歯科医講習会 タスクフォース  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:令和4年9月10~11日

  • 2022  福岡歯科大学  Classification:Part-time lecturer  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:9/12

  • 2022  第25回中国・四国地区歯科医師臨床研修指導歯科医講習会 タスクフォース  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:令和4年10月29,30日

  • 2022  福岡国際医療福祉大学看護学科  Classification:Part-time lecturer  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:2022. 12/2 2023. 1/6

  • 2021  令和3年度鹿児島大学病院歯科医師臨床研修指導歯科医講習会 タスクフォース  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:令和3年7月16~18日

  • 2021  令和3年度九州大学病院歯科医師臨床研修指導歯科医講習会 ディレクター・タスクフォース  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:令和3年10月8~10日

  • 2020  福岡歯科大学  Classification:Part-time lecturer  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:8月31日

  • 2020  第15回大阪歯科大学附属病院歯科医師臨床研修指導歯科医講習会 タスクフォース  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:令和3年1月22日~24日

  • 2020  令和2年度プログラム責任者講習会 タスクフォース  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:令和3年3月1-3日

  • 2020  第24回中国・四国地区歯科医師臨床研修指導歯科医講習会 タスクフォース  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:令和3年3月27,28日

  • 2019  福岡歯科大学  Classification:Part-time lecturer  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:9月30日

  • 2019  2019年度医療法人社団湧泉会ひまわり歯科歯科医師臨床研修指導歯科医講習会 タスクフォース  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:令和元年6月22、23日

  • 2019  14. 第73回医学教育セミナーとワークショップ in 愛知学院大学「プロフェッショナリズム教育を実践しよう」 ファシリテータ  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:令和元年8月10日

  • 2018  徳島大学病院  Classification:Part-time lecturer  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:9月11日

  • 2018  鹿児島大学病院歯科医師臨床研修指導歯科医講習会 タスクフォース  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:平成30年11月10、11日

  • 2018  鹿児島大学病院歯科医師臨床研修指導歯科医講習会 タスクフォース  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:平成31年2月2、3日

  • 2018  第22回中国・四国地区歯科医師臨床研修指導歯科医講習会 タスクフォース  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:平成31年2月17、18日

  • 2018  福岡歯科大学  Classification:Part-time lecturer  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:9月3日

  • 2017  平成29年度福岡歯科大学医科歯科総合病院歯科医師臨床研修指導歯科医講習会 タスクフォース  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:平成29年7月14、15日

  • 2017  広島大学歯学部 共用試験歯学系OSCEモニター委員  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:9月8,9日

  • 2017  鹿児島大学歯学部 臨床実習終了時OSCEモニター  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:9月11日

  • 2017  福岡歯科大学  Classification:Part-time lecturer  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:9月25日

  • 2017  明海大学歯学部 共用試験歯学系OSCEモニター委員  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:2018年2月21,22日

  • 2017  第21回中国・四国地区歯科医師臨床研修指導歯科医講習会 タスクフォース  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:平成29年12月16、17日

  • 2017  平成29年度伊東歯科口腔病院歯科医師臨床研修指導歯科医講習会 タスクフォース  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:平成30年3月17、18日

  • 2016  第20回中国・四国地区歯科医師臨床研修指導歯科医講習会 タスクフォース  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:平成29年2月11、12日

  • 2016  平成28年度福岡歯科大学医科歯科総合病院歯科医師臨床研修指導歯科医講習会 タスクフォース  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:平成28年7月16、17日

  • 2016  広島大学歯学部 OSCE外部評価者  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:9月9,10日

  • 2016  福岡歯科大学  Classification:Part-time lecturer  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:9月26日

  • 2016  鹿児島大学病院歯科医師臨床研修指導歯科医講習会 タスクフォース  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:平成28年10月28、29日

  • 2015  岡山大学 OSCE外部評価者  Domestic/International Classification:Japan 

    Semester, Day Time or Duration:9月18,19日

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Participation in international educational events, etc.

  • 2024.2

    Kyushu University, Faculty of Dental Science

    Kyudai Oral Bioscience (KOB) 2023

      More details

    Venue:Fukuoka

  • 2021.2

    Kyushu University, Faculty of Dental Science

    KOB (Kyudai Oral Biosciences)・OBT 合同国際シンポジウム

      More details

    Venue:Fukuoka

  • 2019.3

    Kyushu University, Faculty of Dental Science

    Kyudai Oral Bioscience (KOB) 2019

      More details

    Venue:Fukuoka

  • 2018.2

    Kyushu University, Faculty of Dental Science

    Kyudai Oral Bioscience (KOB) 2018

      More details

    Venue:Fukuoka

  • 2016.2

    Kyushu University, Faculty of Dental Science

    Kyudai Oral Bioscience (KOB) 2016

      More details

    Venue:Fukuoka

  • 2016.2

    九州大学大学院 歯学研究院 日本学術振興会 頭脳循環を加速する戦略的国際研究ネットワーク推進プログラム 口腔から健康長寿を支えるプロジェクト推進に向けた研究拠点構築プログラム

    頭脳循環第二回シンポジウム

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    Venue:Fukuoka

  • 2015.2

    Faculty of Dental Science, Kyushu University

    Kyudai Oral Bioscience 2013—8th International Symposium —

      More details

    Venue:Fukuoka, Japan

  • 2014.2

    歯学研究院

    Kyudai Oral Bioscience 2013—8th International Symposium —

      More details

    Venue:福岡市

  • 2013.3

    九州大学歯学研究院

    Kyudai Oral Bioscience 2013—7th International Symposium —

      More details

    Venue:福岡市

  • 2011.3

    九州大学大学院歯学研究院

    The 6 th International Joint Symposium on “Dental and Craniofacial Morphogenesis and Tissue Regeneration” and “Oral Health Science”

      More details

    Venue:日本・福岡市

  • 2007.3

    九州大学大学院 歯学研究院

    九州大学大学院 歯学研究院「魅力ある大学院教育イニシアティブ」「口腔顎顔面領域の発生と再生」国際シンポジウム

      More details

    Venue:日本・福岡市

  • 2006.3

    九州大学大学院 歯学研究院

    九州大学大学院 歯学研究院「魅力ある大学院教育イニシアティブ」「口腔顎顔面領域の発生と再生」国際シンポジウム

      More details

    Venue:日本・福岡

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Other educational activity and Special note

  • 2024  Coaching of Students' Association  歯学部野球部

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    顧問

  • 2023  Coaching of Students' Association  歯学部野球部

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    顧問

  • 2023  Special Affairs  令和5年度第1回プログラム責任者講習会(一般社団法人日本歯科医学教育学会主催(厚生労働省補助事業))にタスクフォースとして参加

     詳細を見る

    令和5年度第1回プログラム責任者講習会(一般社団法人日本歯科医学教育学会主催(厚生労働省補助事業))にタスクフォースとして参加

  • 2023  Special Affairs  令和5年度第1回プログラム責任者講習会(一般財団法人歯科医療振興財団主催)にタスクフォースとして参加

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    令和5年度第1回プログラム責任者講習会(一般財団法人歯科医療振興財団主催)にタスクフォースとして参加

  • 2023  Special Affairs  令和5年度第2回プログラム責任者講習会(一般財団法人歯科医療振興財団主催)にタスクフォースとして参加

     詳細を見る

    令和5年度第2回プログラム責任者講習会(一般財団法人歯科医療振興財団主催)にタスクフォースとして参加

  • 2023  Special Affairs  令和5年度第2回プログラム責任者講習会(一般社団法人日本歯科医学教育学会主催(厚生労働省補助事業))にタスクフォースとして参加

     詳細を見る

    令和5年度第2回プログラム責任者講習会(一般社団法人日本歯科医学教育学会主催(厚生労働省補助事業))にタスクフォースとして参加

  • 2022  Coaching of Students' Association  歯学部野球部

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    監督

  • 2022  Special Affairs  令和4年度第1回プログラム責任者講習会(一般財団法人歯科医療振興財団主催)にタスクフォースとして参加

     詳細を見る

    令和4年度第1回プログラム責任者講習会(一般財団法人歯科医療振興財団主催)にタスクフォースとして参加

  • 2022  Special Affairs  令和4年度第2回プログラム責任者講習会(一般財団法人歯科医療振興財団主催)にタスクフォースとして参加

     詳細を見る

    令和4年度第2回プログラム責任者講習会(一般財団法人歯科医療振興財団主催)にタスクフォースとして参加

  • 2022  Special Affairs  令和4年度プログラム責任者講習会(一般社団法人日本歯科医学教育学会主催(厚生労働省補助事業))にタスクフォースとして参加

     詳細を見る

    令和4年度プログラム責任者講習会(一般社団法人日本歯科医学教育学会主催(厚生労働省補助事業))にタスクフォースとして参加

  • 2021  Coaching of Students' Association  歯学部野球部

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    監督

  • 2021  Special Affairs  令和3年度プログラム責任者講習会(一般財団法人歯科医療振興財団主催)にタスクフォースとして参加

     詳細を見る

    令和3年度プログラム責任者講習会(一般財団法人歯科医療振興財団主催)にタスクフォースとして参加

  • 2021  Special Affairs  令和3年度第1回共用試験歯学系OSCE評価者養成ワークショップⅡ(愛知学院大学)にタスクフォースとして参加

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    令和3年度第1回共用試験歯学系OSCE評価者養成ワークショップⅡ(愛知学院大学)にタスクフォースとして参加

  • 2021  Special Affairs  令和3年度第2回共用試験歯学系OSCE評価者養成ワークショップⅡ(広島大学)にタスクフォースとして参加

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    令和3年度第2回共用試験歯学系OSCE評価者養成ワークショップⅡ(広島大学)にタスクフォースとして参加

  • 2021  Special Affairs  令和3年度プログラム責任者講習会(一般社団法人日本歯科医学教育学会主催(厚生労働省補助事業))にタスクフォースとして参加

     詳細を見る

    令和3年度プログラム責任者講習会(一般社団法人日本歯科医学教育学会主催(厚生労働省補助事業))にタスクフォースとして参加

  • 2020  Coaching of Students' Association  歯学部野球部

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    監督

  • 2019  Coaching of Students' Association  歯学部野球部

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    監督

  • 2019  Special Affairs  平成30年度第2回共用試験歯学系OSCE評価者養成ワークショップⅠ(九州歯科大学)にタスクフォースとして参加

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    平成30年度第2回共用試験歯学系OSCE評価者養成ワークショップⅠ(九州歯科大学)にタスクフォースとして参加

  • 2018  Coaching of Students' Association  歯学部野球部

     詳細を見る

    監督

  • 2018  Special Affairs  平成30年度第1回共用試験歯学系OSCE評価者養成ワークショップⅡ(日本歯科大学)にタスクフォースとして参加

     詳細を見る

    平成30年度第1回共用試験歯学系OSCE評価者養成ワークショップⅡ(日本歯科大学)にタスクフォースとして参加

  • 2018  Special Affairs  平成30年度第2回共用試験歯学系OSCE評価者養成ワークショップⅡ(朝日大学)にタスクフォースとして参加

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    平成30年度第2回共用試験歯学系OSCE評価者養成ワークショップⅡ(朝日大学)にタスクフォースとして参加

  • 2018  Special Affairs  平成31年度第2回共用試験歯学系OSCE評価者養成ワークショップⅡ(大阪大学)にタスクフォースとして参加

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    平成31年度第2回共用試験歯学系OSCE評価者養成ワークショップⅡ(大阪大学)にタスクフォースとして参加

  • 2018  Special Affairs  厚生労働省災害医療チーム等養成支援事業災害歯科保健医療体制研修会に参加、修了

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    厚生労働省災害医療チーム等養成支援事業災害歯科保健医療体制研修会に参加、修了

  • 2017  Coaching of Students' Association  歯学部野球部

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    監督

  • 2017  Special Affairs  平成29年度第1回共用試験歯学系OSCE評価者養成ワークショップⅠ(鹿児島大学歯学部)にタスクフォースとして参加

     詳細を見る

    平成29年度第1回共用試験歯学系OSCE評価者養成ワークショップⅠ(鹿児島大学歯学部)にタスクフォースとして参加

  • 2017  Special Affairs  平成29年度第2回共用試験歯学系OSCE評価者養成ワークショップⅠ(大阪歯科大学)にタスクフォースとして参加

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    平成29年度第2回共用試験歯学系OSCE評価者養成ワークショップⅠ(大阪歯科大学)にタスクフォースとして参加

  • 2016  Coaching of Students' Association  歯学部野球部

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    監督

  • 2015  Coaching of Students' Association  歯学部野球部

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    監督

  • 2015  Special Affairs  WS「倫理的検討事例を用いたプロフェッショナリズム教育の展開」(日本歯科医学教育学会主催)参加・修了

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    WS「倫理的検討事例を用いたプロフェッショナリズム教育の展開」(日本歯科医学教育学会主催)参加・修了

  • 2015  Special Affairs  平成27年度プログラム責任者講習会(一般財団法人歯科医療振興財団)参加・修了

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    平成27年度プログラム責任者講習会(一般財団法人歯科医療振興財団)参加・修了

  • 2015  Special Affairs  岡山大学歯学部OSCE外部評価者として参加

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    岡山大学歯学部OSCE外部評価者として参加

  • 2015  Special Affairs  平成27年度第6回歯科医学教育者のためのワークショップ(日本歯科医学教育学会主催)参加・修了

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    平成27年度第6回歯科医学教育者のためのワークショップ(日本歯科医学教育学会主催)参加・修了

  • 2015  Special Affairs  第2回医療コミュニケーションガイド作成に関する会議出席

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    第2回医療コミュニケーションガイド作成に関する会議出席

  • 2014  Special Affairs  平成26年度第1回共用試験歯学系OSCE評価者養成ワークショップII 参加・修了

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    平成26年度第1回共用試験歯学系OSCE評価者養成ワークショップII 参加・修了

  • 2014  Special Affairs  指導大学院生1名:学位取得(歯学博士)

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    指導大学院生1名:学位取得(歯学博士)

  • 2011  Special Affairs  平成23年度第二回共用試験歯学系OSCE評価者養成ワークショップI (社団法人医療系大学間共用試験実施評価機構主催) 参加・修了

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    平成23年度第二回共用試験歯学系OSCE評価者養成ワークショップI (社団法人医療系大学間共用試験実施評価機構主催) 参加・修了

  • 2010  Special Affairs  平成22年度九州大学歯学部共用試験歯学OSCEのステーション責任者として参加

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    平成22年度九州大学歯学部共用試験歯学OSCEのステーション責任者として参加

  • 2010  Special Affairs  2010年8月9日 歯学部オープンキャンパスに参加し、所属分野の臨床および研究内容について講演。

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    2010年8月9日 歯学部オープンキャンパスに参加し、所属分野の臨床および研究内容について講演。

  • 2010  Special Affairs  2010年4月~2011年3月 所属研究分野所属の大学院生の研究技術指導およびアドバイス。

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    2010年4月~2011年3月 所属研究分野所属の大学院生の研究技術指導およびアドバイス。

  • 2007  Special Affairs  CBT問題作成

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    CBT問題作成

  • 2006  Special Affairs  CBT問題作成

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    CBT問題作成

  • 2006  Special Affairs  九州大学病院・歯科医師 臨床研修指導歯科医講習会に参加、修了

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    九州大学病院・歯科医師 臨床研修指導歯科医講習会に参加、修了

  • 2006  Special Affairs  平成18年度九州大学歯学部共用試験歯学OSCEの評価者として参加

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    平成18年度九州大学歯学部共用試験歯学OSCEの評価者として参加

  • 2005  Special Affairs  平成17年度九州大学歯学部共用試験歯学OSCEトライアルの評価者として参加

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    平成17年度九州大学歯学部共用試験歯学OSCEトライアルの評価者として参加

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Outline of Social Contribution and International Cooperation activities

  • ・当科受診患者に対してメンテナンス・リコールを行うことにより口腔ケアの重要性の啓蒙に努める。
    ・学会やシンポジウムあるいメディアを通して歯科医療について国民に発信する。
    ・地域歯科へのサポートを行う。
    ・JICAによる外国人歯科医師のTraining Course in Oral Health Science Educationの歯科保存学分野を担当していた。

Social Activities

  • 令和2年度プログラム責任者講習会にTFとして参加

    一般財団法人歯科医療振興財団主催  オンライン方式  2021.3

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 第24回中国・四国地区歯科医師臨床研修指導歯科医講習会にTFとして参加

    広島大学病院  2021.3

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 第15回大阪歯科大学病院歯科医師臨床研修指導歯科医講習会にTFとして参加

    オンライン  2021.1

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 全身の健康につながるお口の健康 ‐口腔衛生と口腔機能の重要性‐

    九州大学医師会  九州大学医学部百年講堂中ホール(福岡市)  2020.2

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 九州大学医師会市民公開講座「全身の健康につながるお口の健康 ‐口腔衛生と口腔機能の重要性‐」

    九州大学医師会  九州大学医学部百年講堂中ホール(福岡市)  2020.2

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 第23回中国・四国地区歯科医師臨床研修指導歯科医講習会にTFとして参加

    徳島大学病院  2020.2

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 第1回カナザキ歯科指導歯科医講習会にTFとして参加

    カナザキ歯科  2020.2

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 歯内治療および歯内再生研究、そして研修医教育

    九州お茶の水会  博多グリーンホテルアネックス(福岡市)  2019.9

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 14. 第73回医学教育セミナーとワークショップ in 愛知学院大学「プロフェッショナリズム教育を実践しよう」にファシリテータとして参加

    MEDC  2019.8

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 歯内治療の基本と潮流(臨床研修の現場から)

    九州大学歯学部同窓会・北九州支部学術講演会  小倉歯科医師会館(北九州市)  2019.8

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 第61回東区薬剤師会研修会「最新歯科治療」講演

    福岡市東区薬剤師会  2019.6

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 2019年度医療法人社団湧泉会ひまわり歯科歯科医師臨床研修指導歯科医講習会にTFとして参加

    医療法人社団湧泉会ひまわり歯科  2019.6

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 第22回中国・四国地区歯科医師臨床研修指導歯科医講習会にTFとして参加

    岡山大学病院  2019.2

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 第2回鹿児島大学病院歯科医師臨床研修指導歯科医講習会にTFとして参加

    鹿児島大学病院  2019.2

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 第1回鹿児島大学病院歯科医師臨床研修指導歯科医講習会にTFとして参加

    鹿児島大学病院  2018.11

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 平成29年度伊東歯科口腔病院歯科医師臨床研修指導歯科医講習会にTFとして参加

    伊東歯科口腔病院  2018.3

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 平成29年度九州大学病院歯科医師臨床研修指導歯科医講習会を開催(ディレクター)

    九州大学病院  2018.2

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 第21回中国・四国歯科医師臨床研修指導歯科医講習会にTFとして参加

    徳島大学病院  2017.12

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 「エンドを科学する-臨床から基礎-」 講演

    九州臨床再生歯科研究会 平成29~30年度研修会  福岡県歯科医師会館(福岡市)  2017.11

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 第30回九州大学病院医療連携センター講演会 進化する医療連携~急性期病院と連携病院のアライアンス 歯科部門報告:周術期歯科診療とホットライン

    九州大学病院医療連携センター  九州大学医学部百年講堂  2017.10

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 平成29年度福岡歯科大学医科歯科総合病院 指導歯科医講習会にタスクフォースとして参加

    福岡歯科大学医科歯科総合病院  福岡歯科大学  2017.7

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 第20回中国・四国歯科医師臨床研修指導歯科医講習会にTFとして参加

    岡山大学病院  2017.2

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 平成28年度鹿児島大学医学部・歯学部附属病院歯科医師臨床研修指導歯科医講習会にTFとして参加

    鹿児島大学医学部・歯学部附属病院  2016.10

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 平成28年度福岡歯科大学医科歯科総合病院 指導歯科医講習会にタスクフォースとして参加

    福岡歯科大学医科歯科総合病院  福岡歯科大学  2016.7

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 学術講演会「歯科医師臨床研修の場から歯内治療を考える」

    九州大学歯学部同窓会 福岡支部  福岡県歯科医師会館(福岡市)  2016.6

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 平成27年度九州大学病院・歯科医師臨床研修指導歯科医講習会をディレクターとして開催に携わった。

    九州大学病院  2016.2

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 筑紫歯科医師会臨床座談会 「エンドを科学する」 講演

    筑紫歯科医師会  2015.12

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 臨床医のための症例検討セミナー「効率的で効果の高い根管治療」 根管治療の効率を考えるの講演

    九州大学歯学部同窓会  2015.11

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Lecture

  • 平成27年度鹿児島大学医学部・歯学部附属病院歯科医師臨床研修指導歯科医講習会にアドバイザーとして参加

    鹿児島大学医学部・歯学部附属病院  2015.10

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • 第18回中国・四国歯科医師臨床研修指導歯科医講習会にオブザーバーとして参加

    徳島大学病院  2015.6

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    Audience:General, Scientific, Company, Civic organization, Governmental agency

    Type:Seminar, workshop

  • カネミ油症検診

    2012

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    カネミ油症検診

  • カネミ油症検診

    2011

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    カネミ油症検診

  • カネミ油症検診

    2010

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    カネミ油症検診

  • ・当科受診患者に対してメンテナンス・リコールを行うことにより口腔ケアの重要性の啓蒙に努めた。 ・当科診療パンフレット作成を担当した。 ・地域歯科へのサポートを兼業により行った。

    2006

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    ・当科受診患者に対してメンテナンス・リコールを行うことにより口腔ケアの重要性の啓蒙に努めた。
    ・当科診療パンフレット作成を担当した。
    ・地域歯科へのサポートを兼業により行った。

  • カネミ油症歯科検診

    2004

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    カネミ油症歯科検診

  • カネミ油症歯科検診

    2003

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    カネミ油症歯科検診

  • カネミ油症歯科検診

    2002

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    カネミ油症歯科検診

▼display all

Media Coverage

  • 象牙質知覚過敏症をテーマにした放送で、症状、原因、発症メカニズムや治療法について説明した内容。 TV or radio program

    九州朝日放送(KBC) 「とっても健康ランド」  2014.6

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    象牙質知覚過敏症をテーマにした放送で、症状、原因、発症メカニズムや治療法について説明した内容。

Educational Activities for Highly-Specialized Professionals in Other Countries

  • 2010.4 - 2010.8   JICA Training Course in Oral Health Science Education

    Main countries of student/trainee affiliation:Sri Lanka

    Other countries of student/trainee affiliation:Uruguay, Chile, Samoa

Travel Abroad

  • 2007.9 - 2010.3

    Staying countory name 1:Australia   Staying institution name 1:Colgate Australian Clinical Dental Research Centre, School of Dentistry, the University of Adelaide

    Staying institution name 2:Mesenchymal Stem Cell Group, Bone and Cancer Laboratories, Department of Haematology, Institute of Medical & Veterinary Science/ Hanson Institute

Specialized clinical area

  • Biology / Medicine, Dentistry and Pharmacy / Dentistry / Conservative Dentistry

  • Biology / Medicine, Dentistry and Pharmacy / Dentistry / Social Dentistry

Clinician qualification

  • Specialist

    日本歯科保存学会

  • Preceptor

    日本歯科保存学会

  • Certifying physician

    日本総合歯科学会

  • Preceptor

    日本総合歯科学会

Year of medical license acquisition

  • 1997

Notable Clinical Activities

  • 外来診療では歯科保存分野全般:修復、歯周、歯内を専門とした包括的治療を行っている。また、卒後研修医および学部学生への臨床教育も行っている。