Updated on 2024/10/09

Information

 

写真a

 
SATO HIROSHI
 
Organization
Faculty of Dental Science Department of Dental Science Assistant Professor
Title
Assistant Professor
Contact information
メールアドレス
Tel
0926426402
Profile
歯髄幹細胞を用いた、染色体異常の病態解析 歯の発生機構の分子生物学的解析
External link

Degree

  • PhD

Research History

  • 岡山大学（博士研究員） 久留米大学（博士研究員、助教）

Research Interests・Research Keywords

  • Research theme：Analysis of the molecular mechanism underlying tooth development

    Keyword：ameloblast

    Research period： 2021.11 - 2023.3

  • Research theme：Study of genetic disease by stem cells from human exfoliated deciduous teeth

    Keyword：SHED

    Research period： 2015.5 - 2023.3

Papers

  • Expression patterns of keratin family members during tooth development and the role of <i>keratin 17</i> in cytodifferentiation of stratum intermedium and stellate reticulum Reviewed

    Inada, S; Chiba, Y; Tian, T; Sato, H; Wang, X; Yoshizaki, K; Oka, S; Yamada, A; Fukumoto, S

    JOURNAL OF CELLULAR PHYSIOLOGY   e31387   2024.7   （ ISSN:0021-9541 eISSN:1097-4652 ）

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    Language：English   Publisher：Journal of Cellular Physiology  

    Keratins are typical intermediate filament proteins of the epithelium that exhibit highly specific expression patterns related to the epithelial type and stage of cellular differentiation. They are important for cytoplasmic stability and epithelial integrity and are involved in various intracellular signaling pathways. Several keratins are associated with enamel formation. However, information on their expression patterns during tooth development remains lacking. In this study, we analyzed the spatiotemporal expression of keratin family members during tooth development using single-cell RNA-sequencing (scRNA-seq) and microarray analysis. scRNA-seq datasets from postnatal Day 1 mouse molars revealed that several keratins are highly expressed in the dental epithelium, indicating the involvement of keratin family members in cellular functions. Among various keratins, keratin 5 (Krt5), keratin 14 (Krt14), and keratin 17 (Krt17) are highly expressed in the tooth germ; KRT17 is specifically expressed in the stratum intermedium (SI) and stellate reticulum (SR). Depletion of Krt17 did not affect cell proliferation in the dental epithelial cell line SF2 but suppressed their differentiation ability. These results suggest that Krt17 is essential for SI cell differentiation. Furthermore, scRNA-seq results indicated that Krt5, Krt14, and Krt17 exhibited distinct expression patterns in ameloblast, SI, and SR cells. Our findings contribute to the elucidation of novel mechanisms underlying tooth development.

    DOI： 10.1002/jcp.31387

    Web of Science

    Scopus

    PubMed

  • TRPV4‐mediated Ca2+ deregulation causes mitochondrial dysfunction via the AKT/α‐synuclein pathway in dopaminergic neurons Reviewed

    2023.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    <jats:title>Abstract</jats:title><jats:p>Mutations in the gene encoding the transient receptor potential vanilloid member 4 (TRPV4), a Ca<jats:sup>2+</jats:sup> permeable nonselective cation channel, cause TRPV4‐related disorders. TRPV4 is widely expressed in the brain; however, the pathogenesis underlying TRPV4‐mediated Ca<jats:sup>2+</jats:sup> deregulation in neurodevelopment remains unresolved and an effective therapeutic strategy remains to be established. These issues were addressed by isolating mutant dental pulp stem cells from a tooth donated by a child diagnosed with metatropic dysplasia with neurodevelopmental comorbidities caused by a gain‐of‐function TRPV4 mutation, c.1855C > T (p.L619F). The mutation was repaired using CRISPR/Cas9 to generate corrected isogenic stem cells. These stem cells were differentiated into dopaminergic neurons and the pharmacological effects of folic acid were examined. In mutant neurons, constitutively elevated cytosolic Ca<jats:sup>2+</jats:sup> augmented AKT‐mediated α‐synuclein (α‐syn) induction, resulting in mitochondrial Ca<jats:sup>2+</jats:sup> accumulation and dysfunction. The TRPV4 antagonist, AKT inhibitor, or α‐syn knockdown, normalizes the mitochondrial Ca<jats:sup>2+</jats:sup> levels in mutant neurons, suggesting the importance of mutant TRPV4/Ca<jats:sup>2+</jats:sup>/AKT‐induced α‐syn in mitochondrial Ca<jats:sup>2+</jats:sup> accumulation. Folic acid was effective in normalizing mitochondrial Ca<jats:sup>2+</jats:sup> levels via the transcriptional repression of α‐syn and improving mitochondrial reactive oxygen species levels, adenosine triphosphate synthesis, and neurite outgrowth of mutant neurons. This study provides new insights into the neuropathological mechanisms underlying TRPV4‐related disorders and related therapeutic strategies.</jats:p>

    DOI： 10.1096/fba.2023-00057

  • Effects of melatonin on dopaminergic neuron development via IP3-mediated mitochondrial Ca2+ regulation in autism spectrum disorder. Reviewed

    Dong S, Kifune T, Kato H, Wang L, Kong J, Hirofuji Y, Xiao Sun, Sato H, Ito Y, Kato TA, Sakai Y, Ohga S, Fukumoto S, Masuda K

    Biochemical and biophysical research communications   2023.9

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    Language：Others   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrc.2023.09.050

  • Deficiency of G protein-coupled receptor Gpr111/Adgrf2 causes enamel hypomineralization in mice by alteration of the expression of kallikrein-related peptidase 4 (Klk4) during pH cycling process. Invited Reviewed International journal

    Yuta Chiba, Keigo Yoshizaki, Hiroshi Sato, Tomoko Ikeuchi, Craig Rhodes, Mitsuki Chiba, Kan Saito, Takashi Nakamura, Tsutomu Iwamoto, Aya Yamada, Yoshihiko Yamada, Satoshi Fukumoto

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology   37 ( 4 )   e22861   2023.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1096/fj.202202053R

  • GSK3β阻害剤により誘導される歯原性間葉系幹細胞はエナメル芽細胞分化を制御する(GSK3beta inhibitor-induced dental mesenchymal stem cells regulate ameloblast differentiation) Reviewed

    Yamada Aya, Yoshizaki Keigo, Saito Kan, Ishikawa Masaki, Chiba Yuta, Hoshikawa Seira, Chiba Mitsuki, Hino Ryoko, Maruya Yuriko, Sato Hiroshi, Masuda Keiji, Yamaza Haruyoshi, Nakamura Takashi, Iwamoto Tsutomu, Fukumoto Satoshi

    Journal of Oral Biosciences   64 ( 4 )   400 - 409   2022.12   （ ISSN:1349-0079 ）

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    Language：English   Publisher：(一社)歯科基礎医学会  

    チャンバー内細胞培養系を用いて歯原性間葉系細胞のサブセットが歯原性上皮細胞からエナメル芽細胞への分化に重要であることを発見した。歯髄細胞からGSK3β阻害剤BIOを用いて歯髄幹細胞様細胞を誘導し、歯髄幹細胞により誘導されるエナメル芽細胞分化のメカニズムについて検討した。BIOで誘導された歯髄細胞は間葉系幹細胞マーカーOct3/4およびBcrp1を高発現した。BIOで誘導された人工歯髄幹細胞を用いて、歯髄幹細胞に発現するエナメル芽細胞分化に必要な分子を同定した。歯原性上皮細胞との相互作用を介して歯髄幹細胞でPanx3発現が誘導された。さらにPanx3発現細胞でATP遊離が増加した。ATP刺激は歯原性上皮細胞に受容された。以上の結果から、歯髄幹細胞に発現するPanx3がエナメル芽細胞分化に重要で、Panx3によるATP遊離が上皮間葉系相互作用に寄与すると考えられた。

  • GSK3beta inhibitor-induced dental mesenchymal stem cells regulate ameloblast differentiation. Invited Reviewed International journal

    Aya Yamada, Keigo Yoshizaki, Kan Saito, Masaki Ishikawa, Yuta Chiba, Seira Hoshikawa, Mitsuki Chiba, Ryoko Hino, Yuriko Maruya, Hiroshi Sato, Keiji Masuda, Haruyoshi Yamaza, Takashi Nakamura, Tsutomu Iwamoto, Satoshi Fukumoto

    Journal of oral biosciences   2022.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.job.2022.10.002

  • Mitochondrial Calcium-Triggered Oxidative Stress and Developmental Defects in Dopaminergic Neurons Differentiated from Deciduous Teeth-Derived Dental Pulp Stem Cells with MFF Insufficiency. Reviewed International journal

    Xiao Sun, Shuangshan Dong, Hiroki Kato, Jun Kong, Yosuke Ito, Yuta Hirofuji, Hiroshi Sato, Takahiro A Kato, Yasunari Sakai, Shouichi Ohga, Satoshi Fukumoto, Keiji Masuda

    Antioxidants (Basel, Switzerland)   11 ( 7 )   2022.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/antiox11071361

  • Dopamine-related oxidative stress and mitochondrial dysfunction in dopaminergic neurons differentiated from deciduous teeth-derived stem cells of children with Down syndrome Reviewed

    Xiao Sun, Hiroki Kato, Hiroshi Sato, Xu Han, Yuta Hirofuji, Takahiro A. Kato, Yasunari Sakai, Shouichi Ohga, Satoshi Fukumoto, Keiji Masuda

    FASEB BioAdvances   4 ( 7 )   454 - 467   2022.7   （ eISSN:2573-9832 ）

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    Language：English   Publishing type：Research paper (scientific journal)  

    Down syndrome (DS) is one of the common genetic disorders caused by the trisomy of human chromosome 21 (HSA21). Mitochondrial dysfunction and redox imbalance play important roles in DS pathology, and altered dopaminergic regulation has been demonstrated in the brain of individuals with DS. However, the pathological association of these elements is not yet fully understood. In this study, we analyzed dopaminergic neurons (DNs) differentiated from deciduous teeth-derived stem cells of children with DS or healthy control children. As previously observed in the analysis of a single case of DS, compared to controls, patient-derived DNs (DS-DNs) displayed shorter neurite outgrowth and fewer branches, as well as downregulated vesicular monoamine transporter 2 and upregulated dopamine transporter 1, both of which are key regulators of dopamine homeostasis in DNs. In agreement with these expression profiles, DS-DNs accumulated dopamine intracellularly and had increased levels of cellular and mitochondrial reactive oxygen species (ROS). DS-DNs showed downregulation of non-canonical Notch ligand, delta-like 1, which may contribute to dopamine accumulation and increased ROS levels through DAT1 upregulation. Furthermore, DS-DNs showed mitochondrial dysfunction in consistent with lower expression of peroxisome proliferator-activated receptor-gamma coactivator 1 alpha (PGC-1α) and upregulation of a HSA21-encoded negative regulator of PGC-1α, nuclear receptor-interacting protein 1. These results suggest that dysregulated dopamine homeostasis may participate in oxidative stress and mitochondrial dysfunction of the dopaminergic system in DS.

    DOI： 10.1096/fba.2021-00086

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    PubMed

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  • Impaired neurite development and mitochondrial dysfunction associated with calcium accumulation in dopaminergic neurons differentiated from the dental pulp stem cells of a patient with metatropic dysplasia. Reviewed International journal

    Xiao Sun, Hiroki Kato, Hiroshi Sato, Michiko Torio, Xu Han, Yu Zhang, Yuta Hirofuji, Takahiro A Kato, Yasunari Sakai, Shouichi Ohga, Satoshi Fukumoto, Keiji Masuda

    Biochemistry and biophysics reports   26   100968 - 100968   2021.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrep.2021.100968

  • Accelerated osteoblastic differentiation in patient-derived dental pulp stem cells carrying a gain-of-function mutation of TRPV4 associated with metatropic dysplasia Reviewed

    Xu Han, Hiroki Kato, Hiroshi Sato, Yuta Hirofuji, Satoshi Fukumoto, Keiji Masuda

    Biochemical and Biophysical Research Communications   2020.1

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    Language：Others  

    Accelerated osteoblastic differentiation in patient-derived dental pulp stem cells carrying a gain-of-function mutation of TRPV4 associated with metatropic dysplasia

    DOI： 10.1016/j.bbrc.2019.12.123

  • Novel gain-of-function mutation of TRPV4 associated with accelerated chondrogenic differentiation of dental pulp stem cells derived from a patient with metatropic dysplasia Reviewed

    Kentaro Nonaka, Xu Han, Hiroki Kato, Hiroshi Sato, Haruyoshi Yamaza, Yuta Hirofuji, Keiji Masuda

    Biochemistry and Biophysics Reports   19   2019.9

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    Novel gain-of-function mutation of TRPV4 associated with accelerated chondrogenic differentiation of dental pulp stem cells derived from a patient with metatropic dysplasia
    © 2019 Metatropic dysplasia is a congenital skeletal dysplasia characterized by severe platyspondyly, dumbbell-like deformity of long tubular bones, and progressive kyphoscoliosis with growth. It is caused by mutations in the gene TRPV4, encoding the transient receptor potential vanilloid 4, which acts as a calcium channel. Many heterozygous single base mutations of this gene have been associated with the disorder, showing autosomal dominant inheritance. Although abnormal endochondral ossification has been observed by histological examination of bone in a patient with lethal metatropic dysplasia, the etiology of the disorder remains largely unresolved. As dental pulp stem cells (DPSCs) are mesenchymal stem cells that differentiate into bone lineage cells, DPSCs derived from patients with congenital skeletal dysplasia might be useful as a disease-specific cellular model for etiological investigation. The purpose of this study was to clarify the pathological association between TRPV4 mutation and chondrocyte differentiation by analyzing DPSCs from a patient with non-lethal metatropic dysplasia. We identified a novel heterozygous single base mutation, c.1855C>T in TRPV4. This was predicted to be a missense mutation, p.L619F, in putative transmembrane segment 5. The mutation was repaired by CRISPR/Cas9 system to obtain isogenic control DPSCs for further analysis. The expression of stem cell markers and fibroblast-like morphology were comparable between patient-derived mutant and control DPSCs, although expression of TRPV4 was lower in mutant DPSCs than control DPSCs. Despite the lower TRPV4 expression in mutant DPSCs, the intracellular Ca2+ level was comparable at the basal level between mutant and control DPSCs, while its level was markedly higher following stimulation with 4α-phorbol 12,13-didecanoate (4αPDD), a specific agonist for TRPV4, in mutant DPSCs than in control DPSCs. In the presence of 4αPDD, we observed accelerated early chondrocyte differentiation and upregulated mRNA expression of SRY-box 9 (SOX9) in mutant DPSCs. Our findings suggested that the novel missense mutation c.1855C>T of TRPV4 was a gain-of-function mutation leading to enhanced intracellular Ca2+ level, which was associated with accelerated chondrocyte differentiation and SOX9 upregulation. Our results also suggest that patient-derived DPSCs can be a useful disease-specific cellular model for elucidating the pathological mechanism of metatropic dysplasia.

    DOI： 10.1016/j.bbrep.2019.100648

  • Protective effect of folic acid on vulnerability to oxidative stress in dental pulp stem cells of deciduous teeth from children with orofacial clefts Reviewed

    Yu Zhang, Xiao Sun, Xu Han, Hiroshi Sato, Yuta Hirofuji, K. Masuda

    Biochemical and Biophysical Research Communications   516   127 - 132   2019.8

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    Protective effect of folic acid on vulnerability to oxidative stress in dental pulp stem cells of deciduous teeth from children with orofacial clefts
    © 2019 Elsevier Inc. Orofacial clefts (OFCs) are among the most common congenital craniofacial malformations, including cleft lip with or without cleft palate as the core symptoms. Developmental or functional defects in neural crest cells (NCCs) that contribute to craniofacial morphogenesis are involved in OFC development. Previous studies have suggested that oxidative stress in NCCs is involved in the development of OFCs, suggesting that the anti-oxidative activity of folic acid (FA) could have protective effects. However, studies of human-derived NCCs are limited, as these cells are predominantly active during the embryonic stage. In this study, the effects of oxidative stress and FA were evaluated in human OFCs. In particular, NCC-derived stem cells from human exfoliated deciduous teeth (SHEDs) were obtained from 3 children with non-syndromic cleft lip with cleft palate (CLPs) and from 3 healthy children (CTRLs). Mitochondrial reactive oxygen species (ROS) levels were significantly higher in CLPs than in CTRLs and were associated with lower mRNA expression levels of superoxide dismutase 1 (SOD1) and decreased cell mobility. In addition, significantly greater vulnerability to pyocyanin-induced ROS, mimicking exogenous ROS, was observed in CLPs than in CTRLs. These vulnerabilities to endogenous and exogenous ROS in CLPs were significantly improved by FA. These results indicated that the transcriptional dysregulation of SOD1 in NCCs is an oxidative stress-related pathological factor in OFCs, providing novel evidence for the benefits of perinatal anti-oxidant supplementation, including FA, for the management of these common deformities.

    DOI： 10.1016/j.bbrc.2019.06.031

  • Positive effect of exogenous brain-derived neurotrophic factor on impaired neurite development and mitochondrial function in dopaminergic neurons derived from dental pulp stem cells from children with attention deficit hyperactivity disorder Reviewed

    Huong Thi, Nguyen Nguyen, Hiroki Kato, Hiroshi Sato, Haruyoshi Yamaza, Yasunari Sakai, Shouichi Ohga, Kazuaki Nonaka, Keiji Masuda

    Biochemical and Biophysical Research Communications   513   1048 - 1054   2019.6

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    Positive effect of exogenous brain-derived neurotrophic factor on impaired neurite development and mitochondrial function in dopaminergic neurons derived from dental pulp stem cells from children with attention deficit hyperactivity disorder
    © 2019 Elsevier Inc. Attention deficit hyperactivity disorder (ADHD) is one of the most common neurodevelopmental disorders and is characterized by impaired attention, hyperactivity, and impulsivity. While multiple etiologies are implicated in ADHD, its underlying mechanism(s) remain unclear. Although previous studies have suggested dysregulation of dopaminergic signals, mitochondria, and brain-derived neurotrophic factor (BDNF) in ADHD, few studies have reported these associations directly. Stem cells from human exfoliated deciduous teeth (SHED) can efficiently differentiate into dopaminergic neurons (DNs) and are thus a useful disease-specific cellular model for the study of neurodevelopmental disorders associated with DN dysfunction. This study aimed to elucidate the relationships between DNs, mitochondria, and BDNF in ADHD by analyzing DNs differentiated from SHED obtained from three boys with ADHD and comparing them to those from three typically developing boys. In the absence of exogenous BDNF in the cell culture media, DNs derived from boys with ADHD (ADHD-DNs) exhibited impaired neurite outgrowth and branching, decreased mitochondrial mass in neurites, and abnormal intracellular ATP levels. In addition, BDNF mRNA was significantly decreased in ADHD-DNs. Supplementation with BDNF, however, significantly improved neurite development and mitochondrial function in ADHD-DNs. These results suggest that ADHD-DNs may have impaired neurite development and mitochondrial function associated with insufficient production of BDNF, which may be improved by exogenous BDNF supplementation. Findings such as these, from patient-derived SHED, may contribute to the future development of treatment strategies for aberrant dopaminergic signaling, mitochondrial functioning, and BDNF levels implicated in ADHD pathogenesis.

    DOI： 10.1016/j.bbrc.2019.04.084

  • Osteoblastic differentiation improved by bezafibrate-induced mitochondrial biogenesis in deciduous tooth-derived pulp stem cells from a child with Leigh syndrome. Reviewed

    Han X, Nonaka K, Kato H, Yamaza H, Sato H, Kifune T, Hirofuji Y, Masuda K

    Biochemistry and biophysics reports   17   32 - 37   2019.3

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    Osteoblastic differentiation improved by bezafibrate-induced mitochondrial biogenesis in deciduous tooth-derived pulp stem cells from a child with Leigh syndrome.

    DOI： 10.1016/j.bbrep.2018.11.003

  • Folic acid-mediated mitochondrial activation for protection against oxidative stress in human dental pulp stem cells derived from deciduous teeth. Reviewed

    Zhang Y, Kato H, Sato H, Yamaza H, Hirofuji Y, Han X, Masuda K, Nonaka K

    Biochemical and biophysical research communications   2018.12

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    Folic acid-mediated mitochondrial activation for protection against oxidative stress in human dental pulp stem cells derived from deciduous teeth.

    DOI： 10.1016/j.bbrc.2018.11.169

  • Impaired neurite development associated with mitochondrial dysfunction in dopaminergic neurons differentiated from exfoliated deciduous tooth-derived pulp stem cells of children with autism spectrum disorder. Reviewed

    Nguyen HTN, Kato H, Masuda K, Yamaza H, Hirofuji Y, Sato H, Pham TTM, Takayama F, Sakai Y, Ohga S, Taguchi T, Nonaka K

    Biochemistry and biophysics reports   16   24 - 31   2018.12

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    Impaired neurite development associated with mitochondrial dysfunction in dopaminergic neurons differentiated from exfoliated deciduous tooth-derived pulp stem cells of children with autism spectrum disorder.

    DOI： 10.1016/j.bbrep.2018.09.004

  • Altered development of dopaminergic neurons differentiated from stem cells from human exfoliated deciduous teeth of a patient with Down syndrome. Reviewed

    Pham TTM, Kato H, Yamaza H, Masuda K, Hirofuji Y, Sato H, Nguyen HTN, Han X, Zhang Y, Taguchi T, Nonaka K

    BMC neurology   18 ( 1 )   132   2018.8

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    Altered development of dopaminergic neurons differentiated from stem cells from human exfoliated deciduous teeth of a patient with Down syndrome.

    DOI： 10.1186/s12883-018-1140-2

  • Mitochondrial dysfunction in dopaminergic neurons differentiated from exfoliated deciduous tooth-derived pulp stem cells of a child with Rett syndrome Reviewed

    Saki Hirofuji, Yuta Hirofuji, Hiroki Kato, Keiji Masuda, Haruyoshi Yamaza, Hiroshi Sato, Fumiko Takayama, Michiko Torio, Yasunari Sakai, Shouichi Ohga, Tomoaki Taguchi, Kazuaki Nonaka

    Biochemical and Biophysical Research Communications   498 ( 4 )   898 - 904   2018.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrc.2018.03.077

  • Accelerated dentinogenesis by inhibiting the mitochondrial fission factor, dynamin related protein 1 Reviewed

    Yumiko I. Matsuishi, Hiroki Kato, Keiji Masuda, Haruyoshi Yamaza, Yuta Hirofuji, Hiroshi Sato, Hiroko Wada, Tamotsu Kiyoshima, Kazuaki Nonaka

    Biochemical and Biophysical Research Communications   495 ( 2 )   1655 - 1660   2018.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrc.2017.12.026

  • Direct effects of mitochondrial dysfunction on poor bone health in Leigh syndrome Reviewed

    Hiroki Kato, Xu Han, Haruyoshi Yamaza, Keiji Masuda, Yuta Hirofuji, Hiroshi Sato, Thanh Thi Mai Pham, Tomoaki Taguchi, Kazuaki Nonaka

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   493 ( 1 )   207 - 212   2017.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrc.2017.09.045

  • Engineering of Systematic Elimination of a Targeted Chromosome in Human Cells Reviewed International journal

    Hiroshi Sato, Hiroki Kato, Haruyoshi Yamaza, Keiji Masuda, Huong Thi Nguyen Nguyen, Thanh Thi Mai Pham, Xu Han, Yuta Hirofuji, and Kazuaki Nonaka

    BIOMED RESEARCH INTERNATIONAL   2017.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1155/2017/6037159

  • Mitochondria regulate the differentiation of stem cells from human exfoliated deciduous teeth Reviewed

    Hiroki Kato, Thanh Thi Mai Pham, Haruyoshi Yamaza, Keiji Masuda, Yuta Hirofuji, Xu Han, Hiroshi Sato, Tomoaki Taguchi, Kazuaki Nonaka

    Cell structure and function   42 ( 2 )   105 - 116   2017.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1247/csf.17012

  • Aldehyde dehydrogenase, Ald4p, is a major component of mitochondrial fluorescent inclusion bodies in the yeast Saccharomyces cerevisiae Reviewed

    Yoshiko Misonou, Maiko Kikuchi, Hiroshi Sato, Tomomi Inai, Tsuneyoshi Kuroiwa, Kenji Tanaka, Isamu Miyakawa

    BIOLOGY OPEN   3 ( 5 )   387 - 396   2014.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1242/bio.20147138

  • Epigenetic Inactivation and Subsequent Heterochromatinization of a Centromere Stabilize Dicentric Chromosomes Reviewed

    Hiroshi Sato, Fumie Masuda, Yuko Takayama, Kohta Takahashi, Shigeaki Saitoh

    CURRENT BIOLOGY   22 ( 8 )   658 - 667   2012.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.cub.2012.02.062

  • Morphology and protein composition of the mitochondrial nucleoids in yeast cells lacking Abf2p, a high mobility group protein Reviewed

    Isamu Miyakawa, Miwako Kanayama, Yuka Fujita, Hiroshi Sato

    JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY   56 ( 6 )   455 - 464   2010.12

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  • Biphasic incorporation of centromeric histone CENP-A in fission yeast Reviewed

    Yuko Takayama, Hiroshi Sato, Shigeaki Saitoh, Yuki Ogiyama, Fumie Masuda, Kohta Takahashi

    MOLECULAR BIOLOGY OF THE CELL   19 ( 2 )   682 - 690   2008.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1091/mbc.E07-05-0504

  • Characterization of a Mis12 homologue in Arabidopsis thaliana Reviewed

    H Sato, F Shibata, M Murata

    CHROMOSOME RESEARCH   13 ( 8 )   827 - 834   2005.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s10577-005-1016-3

  • A 22 kDa protein specific for yeast mitochondrial nucleoids is an unidentified putative ribosomal protein encoded in open reading frame YGL068W Reviewed

    H Sato, Miyakawa, I

    PROTOPLASMA   223 ( 2-4 )   175 - 182   2004.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00709-004-0040-z

  • Characterization of a CENP-C homolog in Arabidopsis thaliana Reviewed

    Y Ogura, F Shibata, H Sato, M Murata

    GENES & GENETIC SYSTEMS   79 ( 3 )   139 - 144   2004.6

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  • Isolation of the mitochondrial nucleoids from yeast Kluyveromyces lactis and analyses of the nucleoid proteins Reviewed

    Miyakawa, I, H Sato, Y Maruyama, T Nakaoka

    JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY   49 ( 2 )   85 - 93   2003.4

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  • Simple detection of a yeast mitochondrial DMA-binding protein, Abf2p, on SDS-DNA gels Reviewed

    Miyakawa, I, Y Kitamura, TK Jyozaki, H Sato, T Umezaki

    JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY   46 ( 6 )   311 - 316   2000.12

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Presentations

  • TOOTH SPECIFIC MOLECULE AMELOD INTERACTED WITH ODAM DURING AMELOGENESIS International conference

    Sae Oka, Hiroshi Sato, Yuta Chiba, Saori Inada, Aya Yamada, Satoshi Fukumoto

    29th IAPD Congress, 2023  2023.6 

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    Event date： 2023.6

    Language：English  

    Country：Netherlands  

  • 歯特異的転写因子AmeloD結合タンパク質の酵母Two-Hybrid法によるスクリーニング

    岡桜 恵, 佐藤 浩, 千葉 雄太, 吉崎 恵悟, 福本 敏

    第64回歯科基礎医学会学術大会  2022.9 

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    Event date： 2022.9

    Language：Japanese  

    Venue：徳島大学蔵本キャンパス（徳島県徳島市）   Country：Japan  

  • サイトケラチンの網羅的解析による新たな歯原性上皮細胞マーカーの同定とその機能解析

    稲田幸織，千葉雄太，韓 旭，佐藤 浩，岡 桜恵，福本 敏

    第60回日本小児歯科学会大会  2022.5 

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    Event date： 2022.5

    Language：Japanese  

    Venue：幕張メッセ国際会議場・ハイブリッド形式   Country：Japan  

  • Pathological analysis of autism spectrum disorder using deciduous tooth-derived stem cells.

    2019.5 

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    Event date： 2019.5

    Language：Japanese  

    Country：Japan  

  • 脱落乳歯を活用したミトコンドリア病研究

    加藤大樹、韓旭、佐藤浩、山座治義、増田啓次、廣藤雄太、野中和

    第18回日本ミトコンドリア学会年会、久留米  2018.12 

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    Event date： 2018.12

    Language：Japanese  

    Country：Japan  

  • レット症候群患児の乳歯歯髄幹細胞から分化したドーパミンニューロンに認めたミトコンドリア機能障害

    増田啓次、加藤大樹、廣藤雄太、廣藤早紀、山座治義、佐藤浩、高山扶美子、野中和明

    第40回日本生物学的精神医学会、神戸  2018.9 

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    Event date： 2018.9

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

    Country：Japan  

  • Study of mitochondrial disease pathology using dental pulp stem cells Invited International conference

    Xu Han, Hiroki Kato, Haruyoshi Yamaza, Keiji Masuda, Yuta Hirofuji, Hiroshi Sato, Thanh Thi Mai Pham, Tomoaki Taguchi, Kazuaki Nonaka

    The 29th FUKUOKA INTERNATIONAL SYMPOSIUM ON PEDIATRIC / MATERNAL-CHILD HEALTH RESEARCH Fukuoka  2018.9 

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    Event date： 2018.9

    Language：English   Presentation type：Oral presentation (general)  

    Country：Japan  

  • Potential application of dental pulp stem cells for etiological research of genetic disorders Invited International conference

    Hiroshi Sato

    The 29th FUKUOKA INTERNATIONAL SYMPOSIUM ON PEDIATRIC / MATERNAL-CHILD HEALTH RESEARCH Fukuoka  2018.9 

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    Event date： 2018.9

    Language：English   Presentation type：Oral presentation (general)  

  • HeLa細胞における21番染色体トリソミーのダイソミー化

    佐藤　浩、加藤　大樹、山座　治義、増田　啓次、Huong Thi Nguyen Nguyen, Thanh Thi Mai Pham,　韓 旭、廣藤　雄太、野中　和明

    ConBio 2017 生命科学合同年次大会  2017.12 

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    Event date： 2018.6

    Language：Japanese  

    Country：Japan  

  • Understanding of neuropathology in Down syndrome: An in vitro study utilizing stem cells from exfoliated deciduous teeth Invited International conference

    The 28th FUKUOKA INTERNATIONAL SYMPOSIUM ON PEDIATRIC / MATERNAL-CHILD HEALTH RESEARCH Fukuoka  2017.8 

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    Event date： 2017.8

    Language：English  

    Country：Japan  

  • Increase of mitochondrial activity is crucial for the neuronal differentiation of stem cells from exfoliated deciduous teeth

    2017.6 

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    Event date： 2017.6

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

    Country：Japan  

  • Implication of Dopaminergic Neurodevelopment in the Pathophysiology of Down Syndrome. International conference

    Thanh Thi Mai Pham, Hiroki Kato, Haruyoshi Yamaza, Keiji Masuda, Yuta Hirofuji, Huong Thi Nguyen Nguyen, Hiroshi Sato, Tomoaki Taguchi, Kazuaki Nonaka

    the Gordon Research Conference on Dendrites: Molecules, Structure & Function, Barga, Italy  2017.3 

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    Event date： 2017.3

    Language：English  

    Country：Italy  

  • Etiological research of congenital disorder with stem cells from human exfoliated deciduous teeth Invited International conference

    Hiroshi Sato, Pham Thi Mai Thanh, Xu Han, Hiroki Kato, Haruyoshi Yamaza, Keiji Masuda, Kazuaki Nonaka

    The 27th FUKUOKA INTERNATIONAL SYMPOSIUM ON PEDIATRIC / MATERNAL-CHILD HEALTH RESEARCH Fukuoka  2016.8 

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    Event date： 2016.8

    Language：English  

    Country：Japan  

  • Biological relationship between mitochondrial function and osteogenesis

    Haruyoshi Yamaza, Hiroki Kato, Xu Han, Hiroshi Sato, Thi Mai Thanh, Yuta Hirofuji, Keiji Masuda, Kazuaki Nonaka

    Gordon Research Conference, Musculoskeletal Biology & Bioengineering Andover, New Hampshire, USA  2016.8 

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    Event date： 2016.8

    Language：English  

    Country：Japan  

  • Demand of mitochondrial activity in neuronal differentiation of stem cells from exfoliated deciduous teeth

    Hiroki Kato, Pham Thi Mai Thanh, Haruyoshi Yamaza, Keiji Masuda, Hiroshi Sato, Kazuaki Nonaka

    2016.3 

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    Event date： 2016.3

    Language：English  

    Country：Japan  

  • Application of pulpal stem cell from exfoliated deciduous teeth to study the etiology of Rett Syndrome

    Hiroki Kato, Keiko Watanabe, Haruyoshi Yamaza, Keiji Masuda, Hiroshi Sato, Kazuaki Nonaka

    The Neurobiology of Parenting", Berzelius symposium 90, Stockholm, Sweden  2015.8 

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    Event date： 2015.8

    Language：English  

    Country：Japan  

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MISC

  • サイトケラチンの網羅的解析による新たな歯原性上皮細胞マーカーの同定とその機能解析

    稲田 幸織, 千葉 雄太, 韓 旭, 佐藤 浩, 岡 桜恵, 福本 敏

    小児歯科学雑誌   2022.3

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    Language：Japanese  

  • Dental Pulp-Derived Mesenchymal Stem Cells for Modeling Genetic Disorders.

    Keiji Masuda, Xu Han, Hiroki Kato, Hiroshi Sato, Yu Zhang, Xiao Sun, Yuta Hirofuji, Haruyoshi Yamaza, Aya Yamada, Satoshi Fukumoto

    International journal of molecular sciences   2021.2

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    Language：English  

    DOI： 10.3390/ijms22052269

  • HeLa細胞における21番染色体トリソミーのダイソミー化

    佐藤 浩, 加藤 大樹, 山座 治義, 増田 啓次, Nguyen Huong, Pham Thanh, 韓 旭, 廣藤 雄太, 野中 和明

    生命科学系学会合同年次大会   2017.12

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    Language：Japanese  

  • Switching the centromeres on and off: epigenetic chromatin alterations provide plasticity in centromere activity stabilizing aberrant dicentric chromosomes Reviewed

    Hiroshi Sato, Shigeaki Saitoh

    BIOCHEMICAL SOCIETY TRANSACTIONS   2013.12

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    Language：English  

    DOI： 10.1042/BST20130136

Professional Memberships

  • THE MOLECULAR BIOLOGY SOCIETY OF JAPAN

  • 日本小児歯科学会

  • 歯科基礎医学会

Academic Activities

  • 運営スタッフ

    第61回 日本小児歯科学会大会  （ 長崎県　出島メッセ長崎 ） 2023.5

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    Type：Competition, symposium, etc. 

Research Projects

  • 乳歯歯髄幹細胞を用いた口腔顔面裂奇形における神経発達障害の機序解明と薬剤の探索

    Grant number：22K10248  2022 - 2024

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • 性染色体数異常における発達障害機序の解析　～乳歯歯髄幹細胞による橋渡し研究～

    Grant number：19K10386  2019 - 2021

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • 細胞増殖から分化への転換に連関するミトコンドリア活性制御機構の解明

    2019 - 2021

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

  • 酸化ストレスからみた口唇裂口蓋裂発症機序解明と予防法の開発

    2016 - 2018

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

  • 乳歯で染色体異常疾患を克服するトランスレーショナル研究

    2016 - 2017

    科学研究費助成事業  挑戦的萌芽研究

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    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

  • セントロメアを染色体上の一カ所に調節する機構の解明

    Grant number：15K06958  2015 - 2017

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • セントロメア不活性化の抑制機構の解明

    2012 - 2014

    科学研究費助成事業  若手研究(B)

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • 分裂酵母の新規セントロメア形成系の構築と形成関連因子の解明

    2009 - 2010

    科学研究費助成事業  若手研究(B)

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    Authorship：Principal investigator  Grant type：Scientific research funding

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Class subject

  • 小児口腔医学（低年次）

    2024.4 - 2025.3   Full year

  • 小児歯科学

    2024.4 - 2025.3   Full year

  • リサーチエクスポージャ

    2024.4 - 2025.3   Full year

  • 小児口腔医学（高年次）

    2024.4 - 2025.3   Full year

  • アーリーエクスポージャ

    2024.4 - 2024.6   Spring quarter

  • PBL

    2024.4 - 2024.6   Spring quarter

  • 小児口腔医学（高年次）

    2023.4 - 2024.3   Full year

  • 小児口腔医学

    2023.4 - 2024.3   Full year

  • リサーチエクスポージャ

    2023.4 - 2024.3   Full year

  • 小児口腔医学（低年次）

    2023.4 - 2024.3   Full year

  • PBL

    2023.4 - 2023.6   Spring quarter

  • アーリーエクスポージャ

    2023.4 - 2023.6   Spring quarter

  • 小児口腔医学

    2022.4 - 2023.3   Full year

  • 小児口腔医学（高年次）

    2022.4 - 2023.3   Full year

  • 小児歯科学

    2022.4 - 2023.3   Full year

  • リサーチエクスポージャ

    2022.4 - 2023.3   Full year

  • 小児口腔医学（コア）

    2022.4 - 2023.3   Full year

  • 小児口腔医学（低年次）

    2022.4 - 2023.3   Full year

  • アーリーエクスポージャ

    2022.4 - 2022.6   Spring quarter

  • 障害者歯科学

    2021.10 - 2022.3   Second semester

  • 小児歯科学

    2021.4 - 2022.3   Full year

  • リサーチエクスポージャ

    2021.4 - 2022.3   Full year

  • 小児口腔医学（低年次）

    2021.4 - 2022.3   Full year

  • 小児口腔医学（コア）

    2021.4 - 2022.3   Full year

  • 小児口腔医学臨床実習

    2021.4 - 2022.3   Full year

  • PBL

    2021.4 - 2021.9   First semester

  • アーリーエクスポージャ

    2021.4 - 2021.6   Spring quarter

  • アーリー・リサーチエクスポージャ

    2020.4 - 2021.3   Full year

  • 小児歯科学

    2020.4 - 2021.3   Full year

  • Integrated Dental Science

    2020.4 - 2021.3   Full year

  • Introduction to Oral Biological Research

    2020.4 - 2021.3   Full year

  • Pediatric Dentistry

    2020.4 - 2021.3   Full year

  • Special Need Dentistry

    2020.4 - 2021.3   Full year

  • 口腔保健推進学研究入門

    2020.4 - 2021.3   Full year

  • 小児口腔医学

    2020.4 - 2021.3   Full year

  • 障害者歯科学

    2020.4 - 2021.3   Full year

  • 統合歯科学特論

    2020.4 - 2021.3   Full year

  • 統合歯科学特論

    2019.10 - 2020.3   Second semester

  • 口腔保健推進学研究入門

    2019.10 - 2020.3   Second semester

  • 小児口腔医学

    2019.10 - 2020.3   Second semester

  • Advanced Dental Science Research

    2019.10 - 2020.3   Second semester

  • アーリー・リサーチエクスポージャ

    2019.4 - 2020.3   Full year

  • 小児歯科学

    2019.4 - 2020.3   Full year

  • PBL

    2019.4 - 2019.9   First semester

  • アーリー・リサーチエクスポージャ

    2018.4 - 2019.3   Full year

  • 小児歯科学

    2018.4 - 2019.3   Full year

  • 小児歯科学

    2017.4 - 2018.3   Full year

  • PBL

    2017.4 - 2017.9   First semester

  • 小児歯科学

    2016.4 - 2016.9   First semester

  • 障害者歯科学

    2015.10 - 2016.3   Second semester

  • 小児歯科学

    2015.4 - 2016.3   Full year

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FD Participation

  • 2023.6   Title：科学研究費補助金採択率向上に向けた工夫

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2021.3   Title：九州大学オンライン授業のグッドプラクティス～リアルタイム型授業 編～

    Organizer：University-wide