Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：English   Publisher：Biomolecules  

Deep caries and severe periodontitis cause bone resorption in periodontal tissue, and severe bone resorption leads to tooth loss. Periodontal ligament stem cells (PDLSCs) are important for the healing of defective periodontal tissue. It is increasingly understood that healing of periodontal tissue is mediated through the secretion of trophic factors, particularly exosomes. This study investigated the effects of exosomes from human PDLSCs (HPDLSCs-Exo) on human osteoblast-like cells in vitro and on the healing of rat calvarial bone defects in vivo. HPDLSCs-Exo were isolated and characterized by their particle shape, size (133 ± 6.4 nm), and expression of surface markers (CD9, CD63, and CD81). In vitro results showed that HPDLSCs-Exo promoted the migration, mineralization, and expression of bone-related genes such as alkaline phosphatase (ALP), bone morphogenetic protein 2 (BMP2), osteocalcin (OCN), and osteopontin (OPN) in human osteoblast-like cells. Furthermore, in vivo results showed that more newly formed bone was observed in the HPDLSCs-Exo-treated group than in the non-treated group at the defect sites in rats. These results indicated that HPDLSCs-Exo could promote osteogenesis in vitro and in vivo, and this suggests that HPDLSCs-Exo may be an attractive treatment tool for bone healing in defective periodontal tissue.

DOI： 10.3390/biom14111455

Web of Science

Scopus

PubMed

Language：English   Publisher：Cell Biochemistry and Function  

We aimed to evaluate the materials based on 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate tri-n-butylborane (Super-bond [SB]) and nano hydroxyapatite (naHAp) for the repair of perforation at pulp chamber floor (PPF) in vitro and in vivo models. SB and naHAp were mixed in the mass ratio of 10% or 30% to produce naHAp/SB. Human periodontal ligament stem cells (HPDLSCs) were cultured on resin discs of SB or naHAp/SB to analyze the effects of naHAp/SB on cell adhesion, proliferation, and cementoblastic differentiation. A rat PPF model was treated with SB or naHAp/SB to examine the effects of naHAp/SB on the healing of defected cementum and periodontal ligament (PDL) at the site of PPF. HPDLSCs were spindle-shaped and adhered to all resin discs. Changing the resin from SB to naHAp/SB did not significantly alter cell proliferation. Both 10% and 30% naHAp/SB were more effective than SB in promoting cementoblastic differentiation of HPDLSCs. In the rat PPF model, 30% naHAp/SB was more effective than SB in promoting the formation Sharpey's fiber-like structures with expression of the PDL-related marker and cementum-like structures with expression of cementum-related markers. In conclusion, 30% naHAp/SB can be the new restorative material for PPF because it exhibited the abilities of adhering to dentin and healing of defected periodontal tissue.

DOI： 10.1002/cbf.4058

Web of Science

Scopus

PubMed

Language：Japanese   Publisher：(一社)日本口腔ケア学会  

緒言;造血器悪性腫瘍の患者ではその病態だけでなく,造血細胞移植や化学療法により易感染状態になり,血流感染を発症することがある.起炎菌の一部は口腔常在菌である.本研究では血流感染を発症した患者について,血液培養における口腔常在菌の有無,血球数,口腔内状態について評価したので報告する.対象と方法;対象は2013年4月1日から2020年3月30日までに,鹿児島大学病院血液膠原病内科で造血細胞移植や化学療法を受けた患者とした.検討項目は発症時の白血球数,血小板数,検出菌,口腔粘膜炎と,直近の口腔清掃状態,歯科的疾患の状態とした.結果;対象期間内に延べで257件の血液培養陽性患者を認めた.その内,口腔常在菌が検出された症例は21件であり,18件でミティスグループのレンサ球菌が検出された.発症時の白血球は終末期の症例を除くと最高で360個/μLであり,移植時と化学療法時のいずれでも認め,また化学療法剤の容量も通常量と高用量のいずれでも認められた.口腔粘膜炎を認めた症例は4例で,歯の清掃状態,歯周状態,齲蝕の状態は症例によってさまざまであった.そこで,発症予防のために機械的な口腔清掃に加えて含嗽による化学的な清掃を実施したところ一定の効果を認めた.考察;造血細胞移植や化学療法に伴う骨髄抑制では,口腔粘膜炎の有無にかかわらず,軽度の歯周炎やブラッシングによる口腔粘膜への機械的刺激なども,口腔常在菌による血流感染の原因となる可能性がある.そのため,骨髄抑制期の患者では,これらを考慮した適切な口腔機能管理が重要と考えられた.(著者抄録)

Language：Japanese  

Language：Japanese  

Responsible for pages：pp21-28   Language：Japanese  

Language：Japanese  

Language：English  

Language：Japanese  

Language：Japanese