Updated on 2024/10/06

Information

 

写真a

 
SUGII HIDEKI
 
Organization
Faculty of Dental Science Department of Dental Science Assistant Professor
School of Dentistry Department of Dentistry（Joint Appointment）
Graduate School of Dental Science （Joint Appointment）
Graduate School of Dental Science Department of Dental Science（Joint Appointment）
Title
Assistant Professor
Tel
0926426432
Profile
歯の保存を目的とし、臨床において根尖病変を有する歯や破折歯などの治療を行っている。また、研究においては、歯とその周囲に位置する歯槽骨を連結する結合組織である歯根膜とそれに結合しているセメント質の再生を目的とし、研究に従事している。歯根膜は歯の固定、圧覚、痛覚、固有感覚等の感覚受容、および周囲細胞への栄養供給等の機能を持ち、歯の再生に重要な役割を果たしている。この歯根膜を再生させるため、その治癒を促進する成長因子を同定し、解析を行い、将来的な臨床応用を目指している。さらに近年は、破折歯の保存の際に用いられるスーパーボンドに注目し、バイオアクティブなスーパーボンドの開発に携わり、セメント質誘導型のスーパーボンドの開発を目指している。また、歯根膜幹細胞より分泌されるエクソソームの研究、細胞外マトリックスを用いた歯周組織再生に関わる研究にも従事している。
External link

Degree

  • PhD

Research History

  • Kyushu University Faculty of Dental Science Department of Dental Science  Assistant Professor

    2017.4 - Present

Research Interests・Research Keywords

  • Research theme：The effect of decorin on the healing of damaged periodontal tissue

    Keyword：Decorin, regeneration of periodontal tissue, osteoblastic differentiation

    Research period： 2021.4 - 2023.3

  • Research theme：Development of adhesive materials which induce periodontal regeneration using resin containing nano hydroxyapatie

    Keyword：nano hydroxyapatite, super bond, cementum, sharpy's fiber, periodontal ligament

    Research period： 2020.4 - 2025.3

  • Research theme：Development of the new method of periodontal guided tissue regeneration applying the exosome from periodontal ligament stem cells

    Keyword：Exosome, PDL stem cells, regeneration

    Research period： 2019.12 - 2024.3

  • Research theme：Development of the new method of periodontal guided tissue regeneration applying the selective cell differentiation potency of Activin A

    Keyword：Activin A, Regeneration of periodontal ligament

    Research period： 2017.4 - 2021.3

Papers

  • 4-META/MMA-TBB resin containing nano hydroxyapatite induces the healing of periodontal tissue repair in perforations at the pulp chamber floor Reviewed

    Sugii, H; Yoshida, S; Albougha, MS; Hamano, S; Hasegawa, D; Itoyama, T; Obata, J; Kaneko, H; Minowa, F; Tomokiyo, A; Maeda, H

    CELL BIOCHEMISTRY AND FUNCTION   42 ( 4 )   e4058   2024.6   （ ISSN:0263-6484 eISSN:1099-0844 ）

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    Language：English   Publisher：Cell Biochemistry and Function  

    We aimed to evaluate the materials based on 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate tri-n-butylborane (Super-bond [SB]) and nano hydroxyapatite (naHAp) for the repair of perforation at pulp chamber floor (PPF) in vitro and in vivo models. SB and naHAp were mixed in the mass ratio of 10% or 30% to produce naHAp/SB. Human periodontal ligament stem cells (HPDLSCs) were cultured on resin discs of SB or naHAp/SB to analyze the effects of naHAp/SB on cell adhesion, proliferation, and cementoblastic differentiation. A rat PPF model was treated with SB or naHAp/SB to examine the effects of naHAp/SB on the healing of defected cementum and periodontal ligament (PDL) at the site of PPF. HPDLSCs were spindle-shaped and adhered to all resin discs. Changing the resin from SB to naHAp/SB did not significantly alter cell proliferation. Both 10% and 30% naHAp/SB were more effective than SB in promoting cementoblastic differentiation of HPDLSCs. In the rat PPF model, 30% naHAp/SB was more effective than SB in promoting the formation Sharpey's fiber-like structures with expression of the PDL-related marker and cementum-like structures with expression of cementum-related markers. In conclusion, 30% naHAp/SB can be the new restorative material for PPF because it exhibited the abilities of adhering to dentin and healing of defected periodontal tissue.

    DOI： 10.1002/cbf.4058

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  • Effect of Fibrillin-2 on Differentiation into Periodontal Ligament Stem Cell-Like Cells Derived from Human-Induced Pluripotent Stem Cells Reviewed

    Hamano, S; Yamashita, D; Hasegawa, D; Sugii, H; Itoyama, T; Maeda, H

    STEM CELLS AND DEVELOPMENT   33 ( 9-10 )   228 - 238   2024.5   （ ISSN:1547-3287 eISSN:1557-8534 ）

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    Language：English   Publisher：Stem Cells and Development  

    Periodontal tissue regeneration is important for preserving teeth. Periodontal ligament stem cells (PDLSCs) are useful in periodontal tissue regeneration; however, tooth extraction is required to obtain these cells. Therefore, we focused on induced pluripotent stem (iPS) cells and established a method to obtain PDLSC-like cells from iPS cells. Specifically, we first differentiated iPS cells into neural crest-like cells (iNCs). Next, we obtained PDLSC-like cells (iPDLSCs) by culturing iNCs on extracellular matrix (ECM) derived from human primary periodontal ligament cells (HPDLCs). This differentiation method suggested that ECM derived from HPDLCs is important for iPDLSC differentiation. Thus, we aimed to identify the PDLSC-inducing factor present in HPDLC-derived ECM in this study. We first performed comprehensive analyses of HPDLC genes and identified fibrillin-2 (FBN2), an ECM-related factor. Furthermore, to clarify the effect of FBN2 on iPDLSC differentiation, we cultured iNCs using ECM derived from HPDLCs with FBN2 knocked down. As a result, expression of PDL-related markers was reduced in iNCs cultured on ECM derived from HPDLCs transfected with FBN2 siRNA (iNC-siFBN2) compared with iPDLSCs. Furthermore, the expression of CD105 (a mesenchymal stem cell marker), proliferation ability, and multipotency of iNC-siFBN2 were lower compared with iPDLSCs. Next, we cultured iNCs on FBN2 recombinant protein; however, expression of PDL-related markers did not increase compared with iPDLSC. The present results suggest the critical involvement of FBN2 in inducing iPDLSCs from iNCs when in fact it does not promote iPDLSC differantiation. Therefore, we need to elucidate the entire HPDLC-ECMs, responsible for iPDLSCs induction.

    DOI： 10.1089/scd.2024.0013

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  • Effect of Fibrillin-2 on Differentiation into Periodontal Ligament Stem Cell-Like Cells Derived from Human-Induced Pluripotent Stem Cells Reviewed International journal

    Sayuri Hamano, Diaki Yamashita, Daigaku Hasegawa, Hideki Sugii, Tomohiro Itoyama, Hidefumi Maeda

    Stem Cells Development   2024.4

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Hyaluronic Acid Induction Promotes the Differentiation of Human Neural Crest-like Cells into Periodontal Ligament Stem-like Cells Reviewed

    Alhasan, MA; Tomokiyo, A; Hamano, S; Sugii, H; Ono, T; Ipposhi, K; Yamashita, K; Mardini, B; Minowa, F; Maeda, H

    CELLS   12 ( 23 )   2023.12   （ eISSN:2073-4409 ）

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    Language：English   Publisher：Cells  

    Periodontal ligament (PDL) stem-like cells (PDLSCs) are promising for regeneration of the periodontium because they demonstrate multipotency, high proliferative capacity, and the potential to regenerate bone, cementum, and PDL tissue. However, the transplantation of autologous PDLSCs is restricted by limited availability. Since PDLSCs are derived from neural crest cells (NCs) and NCs persist in adult PDL tissue, we devised to promote the regeneration of the periodontium by activating NCs to differentiate into PDLSCs. SK-N-SH cells, a neuroblastoma cell line that reportedly has NC-like features, seeded on the extracellular matrix of PDL cells for 2 weeks, resulted in the significant upregulation of PDL marker expression. SK-N-SH cell-derived PDLSCs (SK-PDLSCs) presented phenotypic characteristics comparable to induced pluripotent stem cell (iPSC)-derived PDLSCs (iPDLSCs). The expression levels of various hyaluronic acid (HA)-related genes were upregulated in iPDLSCs and SK-PDLSCs compared with iPSC-derived NCs and SK-N-SH cells, respectively. The knockdown of CD44 in SK-N-SH cells significantly inhibited their ability to differentiate into SK-PDLSCs, while low-molecular HA (LMWHA) induction enhanced SK-PDLSC differentiation. Our findings suggest that SK-N-SH cells could be applied as a new model to induce the differentiation of NCs into PDLSCs and that the LMWHA–CD44 relationship is important for the differentiation of NCs into PDLSCs.

    DOI： 10.3390/cells12232743

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  • Hyaluronic Acid Induction Promotes the Differentiation of Human Neural Crest-like Cells into Periodontal Ligament Stem-like Cells Reviewed International journal

    M Anas Alhasan, Atsushi Tomokiyo, Sayuri Hamano, Hideki Sugii, Taiga Ono, Keita Ipposhi, Kozue Yamashita, Bara Mardini, Fumiko Minowa, Hidefumi Maeda

    Cells   2023.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： doi: 10.3390/cells12232743

  • Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells Reviewed

    Fujino, S; Hamano, S; Tomokiyo, A; Sugiura, R; Yamashita, D; Hasegawa, D; Sugii, H; Fujii, S; Itoyama, T; Miyaji, H; Maeda, H

    SCIENTIFIC REPORTS   13 ( 1 )   5668   2023.4   （ ISSN:2045-2322 ）

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    Language：English   Publisher：Scientific Reports  

    Conventional direct pulp-capping materials induce pulp cells to secrete various biomolecules in pulp tissues that promote reparative dentin formation through induction of odontoblastic differentiation of dental pulp stem cells (DPSCs). However, these biomolecules sometimes induce bone-like dentin with poor sealing properties. Therefore, exploration of biomolecules that allow tight sealing by tubular reparative dentin is required. We recently reported that dopamine (DA) is involved in dentinogenesis. Hence, we investigated the effect of DA on odontoblastic differentiation of DPSCs and reparative dentin formation. Both tyrosine hydroxylase (TH), a DA synthetase, and DA were expressed in odontoblast-like cells in vivo. In vitro, their expression was increased during odontoblastic differentiation of DPSCs. Furthermore, TH-overexpressing DPSCs had promoted odontoblastic differentiation and DA production. Moreover, DA stimulation promoted their differentiation and induced tubular reparative dentin. These results suggest that DA produced by TH is involved in odontoblastic differentiation of DPSCs and has an inductive capacity for reparative dentin formation similar to primary dentin. This study may lead to the development of therapy to preserve vital pulp tissues.

    DOI： 10.1038/s41598-023-32126-1

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  • Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells Reviewed International journal

    Shoko Fujino, Sayuri Hamano, Atsushi Tomokiyo, Risa Sugiura, Daiki Yamashita, Daigaku Hasegawa, Hideki Sugii, Shinsuke Fujii, Tomohiro Itoyama, Hirofumi Miyaji, Hidefumi Maeda

    Scientific reports   2023.4

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Decorin Promotes Osteoblastic Differentiation of Human Periodontal Ligament Stem Cells Invited Reviewed International journal

    Adachi, Orie; Sugii, Hideki; Itoyama, Tomohiro; Fujino, Shoko; Kaneko, Hiroshi; Tomokiyo, Atsushi; Hamano, Sayuri; Hasegawa, Daigaku; Obata, Junko; Yoshida, Shinichiro; Kadowaki, Masataka; Sugiura, Risa; Albougha, Mhd Safwan; Maeda, Hidefumi

    MOLECULES   27 ( 23 )   2022.12   （ eISSN:1420-3049 ）

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/molecules27238224

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  • Involvement of M1/M2 Macrophage Polarization in Reparative Dentin Formation Invited Reviewed International journal

    Kadowaki, Masataka; Yoshida, Shinichiro; Itoyama, Tomohiro; Tomokiyo, Atsushi; Hamano, Sayuri; Hasegawa, Daigaku; Sugii, Hideki; Kaneko, Hiroshi; Sugiura, Risa; Maeda, Hidefumi

    LIFE-BASEL   12 ( 11 )   2022.11   （ ISSN:2075-1729 eISSN:2075-1729 ）

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/life12111812

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  • PAX9 Is Involved in Periodontal Ligament Stem Cell-like Differentiation of Human-Induced Pluripotent Stem Cells by Regulating Extracellular Matrix Invited Reviewed International journal

    Sugiura, Risa; Hamano, Sayuri; Tomokiyo, Atsushi; Hasegawa, Daigaku; Yoshida, Shinichiro; Sugii, Hideki; Fujino, Shoko; Adachi, Orie; Kadowaki, Masataka; Yamashita, Daiki; Maeda, Hidefumi

    BIOMEDICINES   10 ( 10 )   2022.10   （ ISSN:2227-9059 eISSN:2227-9059 ）

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/biomedicines10102366

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  • Inhibition of c-Jun N-terminal kinase signaling promotes osteoblastic differentiation of periodontal ligament stem cells and induces regeneration of periodontal tissues Reviewed

    Kaneko, H; Hasegawa, D; Itoyama, T; Yoshida, S; Tomokiyo, A; Hamano, S; Sugii, H; Maeda, H

    ARCHIVES OF ORAL BIOLOGY   134   105323   2022.2   （ ISSN:0003-9969 eISSN:1879-1506 ）

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    Language：English   Publisher：Archives of Oral Biology  

    Objectives: Few clinical treatments to regenerate periodontal tissue lost due to severe endodontic and periodontal disease have yet been developed. Therefore, the development of new treatment methods for the regeneration of periodontal tissue is expected. The purpose of this study was to investigate the effects of a c-Jun N-terminal kinase (JNK) inhibitor, SP600125, on the osteoblastic differentiation of periodontal ligament stem cells (PDLSCs) in vitro, and the function of SP600125 on the regeneration of alveolar bone in vivo. Design: Alizarin red S staining, quantitative RT-PCR, and western blotting analysis was performed to determine whether SP600125 affects osteoblastic differentiation of human PDLSCs (HPDLSCs) and bone-related intracellular signaling. The effect of SP600125 on the regeneration of alveolar bone was assessed by using a rat periodontal defect model. The healing of periodontal defects was evaluated using micro-CT scans and histological analysis. Results: SP600125 promoted the osteoblastic differentiation such as Alizarin red S-positive mineralized nodule formation and the expression of osteoblast-related genes in HPDLSCs under osteogenic conditions. In addition, this inhibitor upregulated the BMP2 expression and the phosphorylation of Smad1/5/8 in HPDLSCs under the same conditions. The inhibition of Smad1/5/8 signaling by LDN193189 suppressed the SP600125-induced osteoblastic differentiation of HPDLSCs. Furthermore, the application of SP600125 promoted the regeneration of not only alveolar bone but also PDL tissue in periodontal defects. Conclusion: This study suggested that inhibition of JNK signaling promotes the osteoblastic differentiation of HPDLSCs through BMP2-Smad1/5/8 signaling, leading to the regeneration of periodontal tissues such as alveolar bone and PDL tissue.

    DOI： 10.1016/j.archoralbio.2021.105323

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  • Secreted Frizzled-Related Protein 1 Promotes Odontoblastic Differentiation and Reparative Dentin Formation in Dental Pulp Cells Invited Reviewed International journal

    Ipposhi, Keita; Tomokiyo, Atsushi; Ono, Taiga; Yamashita, Kozue; Alhasan, Muhammad Anas; Hasegawa, Daigaku; Hamano, Sayuri; Yoshida, Shinichiro; Sugii, Hideki; Itoyama, Tomohiro; Ogawa, Marina; Maeda, Hidefumi

    Cells   2021.12

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  • Activin A Promotes Osteoblastic Differentiation of Human Preosteoblasts through the ALK1-Smad1/5/9 Pathway Invited Reviewed International journal

    Sugii, Hideki; Albougha, Mhd Safwan; Adachi, Orie; Tomita, Hiroka; Tomokiyo, Atsushi; Hamano, Sayuri; Hasegawa, Daigaku; Yoshida, Shinichiro; Itoyama, Tomohiro; Maeda, Hidefumi

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   22 ( 24 )   2021.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/ijms222413491

  • Mineral trioxide aggregate immersed in sodium hypochlorite reduce the osteoblastic differentiation of human periodontal ligament stem cells Invited Reviewed International journal

    Yamashita, Kozue; Tomokiyo, Atsushi; Ono, Taiga; Ipposhi, Keita; Alhasan, M. Anas; Tsuchiya, Akira; Hamano, Sayuri; Sugii, Hideki; Yoshida, Shinichiro; Itoyama, Tomohiro; Maeda, Hidefumi

    SCIENTIFIC REPORTS   11 ( 1 )   2021.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41598-021-01545-3

  • Inhibition of c-Jun N-terminal kinase signaling promotes osteoblastic differentiation of periodontal ligament cells and induces regeneration of periodontal tissues. Reviewed International journal

    Hiroshi K, Daigaku H, Tomohiro I, Shinichiro Y, Atsushi T, Sayuri H, Hideki S, Hidefumi M

    Archives of Oral Biology   2021.11

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  • Development of a novel direct dental pulp-capping material using 4-META/MMA-TBB resin with nano hydroxyapatite Invited Reviewed International journal

    Yoshida, Shinichiro; Sugii, Hideki; Itoyama, Tomohiro; Kadowaki, Masataka; Hasegawa, Daigaku; Tomokiyo, Atsushi; Hamano, Sayuri; Ipposhi, Keita; Yamashita, Kozue; Maeda, Hidefumi

    MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS   130   2021.11

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    DOI： 10.1016/j.msec.2021.112426

  • Iodine potassium iodide が White Mineral Trioxide Aggregate の表面性状および機械 的強度に及ぼす影響について. Reviewed

    山下梢，友清淳，小野太雅，一法師啓太，濱野さゆり，杉井英樹，吉田晋一郎，糸山知宏，前田英史

    日歯内療誌.   2021.8

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • Possible function of GDNF and Schwann cells in wound healing of periodontal tissue Invited Reviewed International journal

    Itoyama, Tomohiro; Yoshida, Shinichiro; Tomokiyo, Atsushi; Hasegawa, Daigaku; Hamano, Sayuri; Sugii, Hideki; Ono, Taiga; Fujino, Shoko; Maeda, Hidefumi

    JOURNAL OF PERIODONTAL RESEARCH   55 ( 6 )   830 - 839   2020.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/jre.12774

  • Functions of beta2-adrenergic receptor in human periodontal ligament cells Invited Reviewed International journal

    Hamano, Sayuri; Tomokiyo, Atsushi; Hasegawa, Daigaku; Yuda, Asuka; Sugii, Hideki; Yoshida, Shinichiro; Mitarai, Hiromi; Wada, Naohisa; Maeda, Hidefum

    JOURNAL OF CELLULAR BIOCHEMISTRY   121 ( 12 )   4798 - 4808   2020.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/jcb.29706

  • MEST Regulates the Stemness of Human Periodontal Ligament Stem Cells Invited Reviewed International journal

    Hasegawa, Daigaku; Hasegawa, Kana; Kaneko, Hiroshi; Yoshida, Shinichiro; Mitarai, Hiromi; Arima, Mai; Tomokiyo, Atsushi; Hamano, Sayuri; Sugii, Hideki; Wada, Naohisa; Kiyoshima, Tamotsu; Maeda, Hidefumi

    STEM CELLS INTERNATIONAL   2020   2020.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1155/2020/9672673

  • 未重合レジンセメントと未重合フロアブルレジンの結合力 Invited Reviewed International journal

    @杉井英樹、@吉田晋一郎、@友清淳、@濱野さゆり、@長谷川大学、@前田英史

    日本歯科保存学会雑誌   2020.5

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  • R-spondin 2 promotes osteoblastic differentiation of immature human periodontal ligament cells through the Wnt/β-catenin signaling pathway Reviewed

    54 ( 2 )   143 - 153   2019.4

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    Objective: In this study, we measured the expression of R-spondin 2 (RSPO2) in periodontal ligament (PDL) tissue and cells. Further, we examined the effects of RSPO2 on osteoblastic differentiation of immature human PDL cells (HPDLCs). Background: R-spondin (RSPO) family proteins are secreted glycoproteins that play important roles in embryonic development and tissue homeostasis through activation of the Wnt/β-catenin signaling pathway. RSPO2, a member of the RSPO family, has been reported to enhance osteogenesis in mice. However, little is known regarding the roles of RSPO2 in PDL tissues. Methods: Expression of RSPO2 in rat PDL tissue and primary HPDLCs was examined by immunohistochemical and immunofluorescence staining, as well as by semiquantitative RT-PCR. The effects of stretch loading on the expression of RSPO2 and Dickkopf-related protein 1 (DKK1) were assessed by quantitative RT-PCR. Expression of receptors for RSPOs, such as Leucine-rich repeat-containing G-protein-coupled receptors (LGRs) 4, 5, and 6 in immature human PDL cells (cell line 2-14, or 2-14 cells), was investigated by semiquantitative RT-PCR. Mineralized nodule formation in 2-14 cells treated with RSPO2 under osteoblastic inductive condition was examined by Alizarin Red S and von Kossa stainings. Nuclear translocation of β-catenin and expression of active β-catenin in 2-14 cells treated with RSPO2 were assessed by immunofluorescence staining and Western blotting analysis, respectively. In addition, the effect of Dickkopf-related protein 1 (DKK1), an inhibitor of Wnt/β-catenin signaling, was also examined. Results: Rat PDL tissue and HPDLCs expressed RSPO2, and HPDLCs also expressed RSPO2, while little was found in 2-14 cells. Expression of RSPO2 as well as DKK1 in HPDLCs was significantly upregulated by exposure to stretch loading. LGR4 was predominantly expressed in 2-14 cells, which expressed low levels of LGR5 and LGR6. RSPO2 enhanced the Alizarin Red S and von Kossa-positive reactions in 2-14 cells. In addition, DKK1 suppressed nuclear translocation of β-catenin, activation of β-catenin, and increases of Alizarin Red S and von Kossa-positive reactions in 2-14 cells, all of which were induced by RSPO2 treatment. Conclusion: RSPO2, which is expressed in PDL tissue and cells, might play an important role in regulating the osteoblastic differentiation of immature human PDL cells through the Wnt/β-catenin signaling pathway.

    DOI： 10.1111/jre.12611

  • Expression and function of dopamine in odontoblasts Reviewed

    Shoko Fujino, Sayuri Hamano, Atsushi Tomokiyo, Tomohiro Itoyama, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Ayako Washio, Aoi Nozu, Taiga Ono, Naohisa Wada, Chiaki Kitamura, Hidefumi Maeda

    Journal of cellular physiology   2019.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/jcp.29314

  • 骨組織上に播種した歯髄幹細胞は歯根膜関連遺伝子を発現する Reviewed

    @吉田晋一郎、@和田尚久、@長谷川大学、@御手洗裕美、@有馬麻衣、@友清淳、@濱野さゆり、@杉井英樹、@前田英史

    日本歯科保存学会誌   2018.12

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  • BMP-IHH-mediated interplay between mesenchymal stem cells and osteoclasts supports calvarial bone homeostasis and repair Reviewed

    Yuxing Guo, Yuan Yuan, Ling Wu, Thach Vu Ho, Junjun Jing, Hideki Sugii, Jingyuan Li, Xia Han, Jifan Feng, Chuanbin Guo, Yang Chai

    Bone Research   6 ( 1 )   2018.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41413-018-0031-x

  • Wnt5a suppresses osteoblastic differentiation of human periodontal ligament stem cell-like cells via Ror2/JNK signaling. Reviewed International journal

    Hasegawa D, Wada N, Yoshida S, Mitarai H, Arima M, Tomokiyo A, Hamano S, Sugii H, Maeda H.

    Journal of cellular phisiology   2018.2

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  • Extracellular Matrix from Periodontal Ligament Cells Could Induce the Differentiation of Induced Pluripotent Stem Cells to Periodontal Ligament Stem Cell-Like Cells. Reviewed International journal

    Hamano S, Tomokiyo A, Hasegawa D, Yoshida S, Sugii H, Mitarai H, Fujino S, Wada N, Maeda H.

    Stem cells and development   2018.1

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  • Senescence and odontoblastic differentiation of dental pulp cells Reviewed

    Aoi Nozu, Sayuri Hamano, Atsushi Tomokiyo, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Hiromi Mitarai, Shuntaro Taniguchi, Naohisa Wada, Hidefumi Maeda

    Journal of cellular physiology   234 ( 1 )   849 - 859   2018.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/jcp.26905

  • Detection, characterization, and clinical application of mesenchymal stem cells in periodontal ligament tissue Reviewed

    Atsushi Tomokiyo, Shinichiro Yoshida, Sayuri Hamano, Daigaku Hasegawa, Hideki Sugii, Hidefumi Maeda

    Stem Cells International   2018   2018.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1155/2018/5450768

  • The DLx5-FGF10 signaling cascade controls cranial neural crest and myoblast interaction during oropharyngeal patterning and development Reviewed

    Hideki Sugii, Alexandre Grimaldi, Jingyuan Li, Carolina Parada, Thach Vu-Ho, Jifan Feng, Junjun Jing, Yuan Yuan, Yuxing Guo, Hidefumi Maeda, Yang Chai

    Development (Cambridge)   144 ( 21 )   4037 - 4045   2017.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1242/dev.155176

  • Calcium-sensing receptor-ERK signaling promotes odontoblastic differentiation of human dental pulp cells. Reviewed International journal

    Mizumachi H, Yoshida S, Tomokiyo A, Hasegawa D, Hamano S, Yuda A, Sugii H, Serita S, Mitarai H, Koori K, Wada N, Maeda H.

    Bone   2017.8

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    Language：English   Publishing type：Research paper (scientific journal)  

  • 歯内治療ならびに修復処置関連溶液によって生じるWhite Mineral Trioxide Aggregateの色調変化に関する比較分析 Reviewed

    友清淳、濱野さゆり、長谷川大学、杉井英樹、吉田晋一郎、御手洗裕美、有馬麻衣、野津葵、和田尚久、前田英史

    日本歯科保存学会誌   2017.8

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • Transforming growth factor-β-induced gene product-h3 inhibits odontoblastic differentiation of dental pulp cells. Reviewed International journal

    2017.6

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • Mechanical induction of interleukin-11 regulates osteoblastic/cementoblastic differentiation of human periodontal ligament stem/progenitor cells. Reviewed International journal

    Monnouchi S, Maeda H, Yuda A, Hamano S, Wada N, Tomokiyo A, Koori K, Sugii H, Serita S, Akamine A.

    Journal of Periodontal Reserch   2015.4

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • Effects of Activin A on the phenotypic properties of human periodontal ligament cells Reviewed

    Hideki Sugii, Hidefumi Maeda, Atsushi Tomokiyo, Naohide Yamamoto, Naohisa Wada, Katsuaki Koori, Daigaku Hasegawa, Sayuri Hamano, Asuka Yuda, Satoshi Monnouchi, Akifumi Akamine

    Bone   66   62 - 71   2014.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bone.2014.05.021

  • Expression and effects of epidermal growth factor on human periodontal ligament cells. Reviewed International journal

    Teramatsu Y, Maeda H, Sugii H, Tomokiyo A, Hamano S, Wada N, Yuda A, Yamamoto N, Koori K, Akamine A

    Cell and Tissue Research   2014.9

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    Language：English   Publishing type：Research paper (scientific journal)  

  • The roles of calcium-sensing receptor and calcium channel in osteogenic differentiation of undifferentiated periodontal ligament cells. Reviewed International journal

    Koori K, Maeda H, Fujii S, Tomokiyo A, Kawachi G, Hasegawa D, Hamano S, Sugii H, Wada N, Akamine A

    Cell and Tissue Reserch   2014.9

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    Language：English   Publishing type：Research paper (scientific journal)  

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Books

  • 「可逆性または不可逆性歯髄炎の診査・診断」編集：林美加子・二階堂徹「接着・機能性材料を活用した歯髄保護ー痛みのない信頼の歯科医療のために」

    @杉井英樹、@前田英史（ Role： Joint author）

    日本歯科評論2020年増刊  2020.7 

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    Language：Japanese   Book type：Scholarly book

Presentations

  • The 4-META/MMA-TBB resin containing nano hydroxyapatite regenerates wounded periodontal tissue at the perforation site Invited International conference

    Hideki Sugii, Shinichiro Yoshida, Sayuri Hamano, Daigaku Hasegawa, Junko Obata, Tomohiro Itoyama, Mhd Safwan Albougha, Fumiko Minowa, Atsushi Tomokiyo, Hidefumi Maeda

    Asian Pacific Endodontic Confederation (APEC) 2023  2023.8 

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    Event date： 2023.8

    Language：English   Presentation type：Oral presentation (general)  

    Country：Taiwan, Province of China  

  • セメント質誘導型ナノハイドロキシアパタイト含有レジンの新規開発

    杉井英樹、吉田晋一郎、Mhd Safwan Albougha、足立織利恵、友清淳、濱野さゆり、長谷川大学、前田英史

    第22回日本再生医療学会総会  2023.3 

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    Event date： 2023.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：国立京都国際会館   Country：Japan  

  • Exosome derived human periodontal ligament stem cells promote bone formation of rat calvarial bone defects

    Mhd Safwan Albougha, Hideki Sugii, Orie Adachi, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Junko Obata, Shinichiro Yoshida, Tomohiro Itoyama, Shoko Fujino, Hiroshi Kaneko, Hidefumi Maeda

    The 156th meeting of Japanese society of conservative dentistry  2022.6 

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    Event date： 2023.6

    Language：English   Presentation type：Oral presentation (general)  

    Country：Japan  

  • Nano Hydroxyapatite含有4-META/MMA-TBBレジンがヒト歯根膜幹細胞のセメント芽細胞様分化に及ぼす影響について

    杉井英樹、吉田晋一郎、Mhd Safwan Albougha, 足立織利恵、友清淳、前田英史

    第４３回日本歯内療法学会学術大会  2022.7 

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    Event date： 2022.7

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：Web開催   Country：Japan  

  • Decorinによるヒト歯根膜幹細胞の骨芽細胞様分化誘導に関する分子機構の解明

    #足立織利恵、@杉井英樹、@糸山知宏、@兼子大志、@友清淳、@濱野さゆり、@長谷川大学、@小幡純子、@吉田晋一郎、@藤野翔香、#Mhd Safwan Albougha、@前田英史

    第１５６回日本歯科保存学会学術大会  2022.6 

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    Event date： 2022.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：Web開催   Country：Japan  

  • Activin A mediates the ALK1-Smad1/5/9 pathway during osteoblastic differentiation of human pre-osteoblasts. International conference

    Hideki Sugii, Mhd Safwan Albougha, Orie Adachi, Hiroka Tomita, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Shinichiro Yoshida, Junko Obata, Tomohiro Itoyama, Taiga Ono, Keita Ippoushi and Hidefumi Maeda.

    2021.7 

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    Event date： 2021.12

    Language：English  

    Country：United States  

  • Activin A がヒト歯髄細胞の象牙芽細胞様分化に及ぼす影響について

    富田洋嘉、杉井英樹、門脇正敬、Mhd Safwan Albougha、足立織利恵、友清淳、濱野さゆり、 長谷川大学、吉田晋一郎、糸山知宏、小幡純子、前田英史．

    第155回日本歯科保存学会秋季学術大会  2021.11 

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    Event date： 2021.12

    Language：Japanese  

    Venue：Web   Country：Japan  

  • Activin A によるヒト前骨芽細胞の分化誘導 に関わるシグナル伝達の解析

    杉井英樹、Mhd Safwan Albougha、足立織利恵、富田洋嘉、友清淳、濱野さゆり、長谷川大 学、吉田晋一郎、糸山知宏、小幡純子、前田英史．

    第155回日本歯科保存学会秋季学術大会  2021.11 

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    Event date： 2021.12

    Language：Japanese  

    Venue：Web   Country：Japan  

  • 歯性上顎洞炎を併発した根尖性 歯周炎を有する上顎第一大臼歯に意図的再植術を行った一症例

    杉井英樹、足立織利恵、富田洋嘉、友清淳、前田英史.

    第 24 回日本歯科医学会学術大会（第 42 回日本歯内療法学会学術大会）  2021.9 

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    Event date： 2021.12

    Language：Japanese  

    Venue：Web   Country：Japan  

  • Nano Hydroxyapatite 含有 4-META/MMA-TBB レジンは、Calcium-sensing receptor-MEK/ERK 経路を介してヒト歯髄幹細胞の象牙芽細胞様分化を促進する．

    吉田晋一郎、杉井英樹、糸山知宏、門脇正敬、一法師啓太、山下梢、友清淳、濱野さゆり、長谷 川大学、前田英史．

    第 154 回日本歯科保存学会春季学術大会．  2021.6 

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    Event date： 2021.12

    Language：Japanese  

    Venue：Web   Country：Japan  

  • Decorinが未分化なヒト歯根膜幹細胞の骨芽細胞様分化に及ぼす影響

    #足立織利恵、@杉井英樹、@糸山知宏、@友清淳、@濱野さゆり、@長谷川大学、@吉田晋一郎、#Mhd Safwan Albougha、@前田英史

    第153回日本歯科保存学会秋季学術大会  2020.11 

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    Event date： 2021.11 - 2020.11

    Language：Japanese  

    Venue：Web   Country：Japan  

  • Exosomes derived from human periodontal ligament stem cells promote osteoblastic differentiation of pre-osteoblasts.

    Mhd Safwan Albougha, Hideki Sugii, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Shinichiro Yoshida, Tomohiro Itoyama, Shoko Fujino, Taiga Ono, Orie Adachi, Hidefumi Maeda

    2019.11 

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    Event date： 2019.12

    Language：English  

    Country：Japan  

  • Activin Aが有する二極性の細胞分化誘導能に関する分子機構の解明

    杉井英樹、友清淳、濱野さゆり、長谷川大学、吉田晋一郎、Mhd Safwan Albougha、前田英史．

    日本歯科保存学会2019年度春季学術大会  2019.6 

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    Event date： 2019.12

    Language：Japanese  

    Venue：石川県立音楽堂 (金沢市)   Country：Japan  

  • Dlx5が軟口蓋の発達に及ぼす影響

    杉井英樹

    九州大学歯学部創立５０周年記念シンポジウム  2017.11 

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    Event date： 2018.8

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

    Venue：九州大学医学部百年講堂（福岡市）   Country：Japan  

  • Activin A reversely works on the osteoblastic differentiation in human pre-osteoblastic cells and periodontal ligament cells. International conference

    Hideki Sugii, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Shinichiro Yoshida, Hiromi Mitarai, Aoi Nozu, Mai Arima, Tomohiro Itoyama, Taiga Ono, Shoko Fujino, Keita Ipposhi, Naohisa Wada, Hidefumi Maeda

    The 65th Annual Meeting of Japanese Association for Dental Research  2017.11 

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    Event date： 2018.8

    Language：English   Presentation type：Oral presentation (general)  

    Country：Japan  

  • Nano Hydroxyapatite含有4-Meta/MMA-TBBレジンがヒト歯髄幹細胞に及ぼす影響について

    吉田晋一郎、杉井英樹、友清淳、長谷川大学、糸山知宏、御手洗裕美、有馬麻衣、濱野さゆり、野津葵、和田尚久、前田英史

    第147回日本歯科保存学会  2017.10 

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    Event date： 2018.8

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：マリオス（盛岡）   Country：Japan  

  • R-spondin2はカノニカルWntシグナルｗ介して未分化なヒト歯根膜細胞の骨芽細胞様分化を促進する

    有馬麻衣、長谷川大学、吉田晋一郎、御手洗裕美、友清淳、濱野さゆり、杉井英樹、和田尚久、前田英史

    第147回日本歯科保存学会  2017.10 

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    Event date： 2018.8

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：マリオス（盛岡市）   Country：Japan  

  • Dlx5 plays a critical role during soft palate muscle development. International conference

    Hideki Sugii, Alexandre Glimaldi, Jingyuan Li, Carolina Parada, Tach-Vu Ho, Jifan Feng, Junjun Jing, Yuan Yuan, Yuxing Guo, Hidefumi Maeda, Yang Chai

    96th General Session & Exhibition of IADR  2018.7 

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    Event date： 2018.7

    Language：English  

    Country：Japan  

  • Activin Aがヒト歯根膜細胞およびヒト前骨芽細胞の骨芽細胞様分化に及ぼす影響

    杉井英樹、友清淳、濱野さゆり、長谷川大学、吉田晋一郎、御手洗裕美、野津葵、有馬麻衣、糸山知宏、小野太雅、藤野翔香、一法師啓太、和田尚久、前田英史

    日本歯科保存学会  2018.6 

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    Event date： 2018.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：横浜みなとみらいホール   Country：Japan  

  • Nano Hydroxyapatite含有4-META/MMA-TBBレジンがヒト歯根膜幹細胞のセメント芽細胞様分化に及ぼす影響について

    杉井 英樹, 吉田 晋一郎, Albougha Mhd Safwan, 足立 織利恵, 友清 淳, 前田 英史

    日本歯内療法学会学術大会プログラム・抄録集  2022.7  (一社)日本歯内療法学会

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    Language：Japanese  

  • フェノール系貼薬剤はケイ酸カルシウムの体積増加を介してWhite Mineral Trioxide Aggregateの崩壊を誘導し封鎖性を低下させる

    友清 淳, 山下 梢, 小野 太雅, 一法師 啓太, Alhasan M. Anas, 濱野 さゆり, 長谷川 大学, 杉井 英樹, 前田 英史

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2022.10  (NPO)日本歯科保存学会

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    Language：Japanese  

  • ヒト歯周靱帯幹細胞由来のエキソソームはラットの頭蓋冠骨欠損に対して骨形成を促進する(Exosomes derived from human periodontal ligament stem cells promote bone formation of rat calvarial bone defects)

    Albougha Mhd Safwan, Sugii Hideki, Adachi Orie, Tomokiyo Atsushi, Hamano Sayuri, Hasegawa Daigaku, Obata Junko, Yoshida Shinichiro, Itoyama Tomohiro, Fujino Shoko, Kaneko Hiroshi, Maeda Hidefumi

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2022.5  (NPO)日本歯科保存学会

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    Language：English  

  • ヒアルロン酸はヒト神経堤様細胞から歯周靱帯幹細胞への分化を促進する(Hyaluronic Acid Promotes the Differentiation of Human Neural Crest-like Cells into Periodontal Ligament Stem-like Cells)

    Alhasan MAnas, 友清 淳, 小野 太雅, 一法師 啓太, 山下 梢, 濱野 さゆり, 長谷川 大学, 杉井 英樹, 前田 英史

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2022.10  (NPO)日本歯科保存学会

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    Language：English  

  • iPS細胞由来歯根膜幹細胞様細胞の分化誘導におけるFibrillin-2の役割

    濱野 さゆり, 山下 大輝, 糸山 知宏, 友清 淳, 長谷川 大学, 杉井 英樹, 兼子 大志, Mardini Bara, 前田 英史

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2023.5  (NPO)日本歯科保存学会

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    Language：Japanese  

  • Decorinによるヒト歯根膜幹細胞の骨芽細胞様分化誘導に関する分子機構の解析

    足立 織利恵, 杉井 英樹, 糸山 知宏, 兼子 大志, 友清 淳, 濱野 さゆり, 長谷川 大学, 小幡 純子, 吉田 晋一郎, 藤野 翔香, Albougha Mhd Safwan, 前田 英史

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2022.5  (NPO)日本歯科保存学会

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    Language：Japanese  

  • AhRシグナルの活性化はヒト歯根膜細胞のMMP12発現を亢進する

    門脇 正敬, 友清 淳, 糸山 知宏, 長谷川 大学, 濱野 さゆり, 杉井 英樹, 前田 英史

    特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集  2023.5  (NPO)日本歯科保存学会

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    Language：Japanese  

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MISC

  • Insight into the Role of Dental Pulp Stem Cells in Regenerative Therapy. Reviewed

    Yoshida S, Tomokiyo A, Hasegawa D, Hamano S, Sugii H, Maeda H

    Biology(Basel)   2020.7

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    Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

    DOI： 10.3390/biology9070160

  • Detection, Characterization, and Clinical Application of Mesenchymal Stem Cells in Periodontal Ligament Tissue.

    Tomokiyo A, Yoshida S, Hamano S, Hasegawa D, Sugii H, Maeda H

    Stem Cell Int   2018.8

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    Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  • Periodontal Ligament Stem Cells in Regenerative Dentistry for Periodontal Tissues.

    Atsushi Tomokiyo, Naohisa Wada, Sayuri Hamano, Daigaku Hasegawa, Hideki Sugii, Shinichiro Yoshida, Hidefumi Maeda

    Journal of Stem Cell Research & Therapy   2016

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    Language：English  

Professional Memberships

  • 日本歯内療法学会

  • 日本歯科保存学会

  • IADR

  • 日本再生医療学会

Committee Memberships

  • Steering committee member   Domestic

    2024.4 - 2026.3   

  • 日本歯内療法学会   国際交流委員会   Domestic

    2024.4 - 2026.3   

  • Organizer   Domestic

    2023.4 - 2025.3   

  • Organizer   Domestic

    2023.4 - 2025.3   

  • 日本歯科保存学会   定款委員会幹事   Domestic

    2023.4 - 2025.3   

  • 日本歯科保存学会   表彰委員会幹事   Domestic

    2023.4 - 2025.3   

  • Organizer   Domestic

    2019.4 - 2023.3   

  • 歯科保存学会   歯科保存学会あり方委員会幹事   Domestic

    2019.4 - 2023.3   

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Academic Activities

  • コメンテーター

    第22回日本再生医療学会総会  （ 国立京都国際会館 ） 2023.3

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    Type：Competition, symposium, etc. 

  • Screening of academic papers

    Role(s)： Peer review

    2023

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    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：4

  • Screening of academic papers

    Role(s)： Peer review

    2022

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    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：4

  • Screening of academic papers

    Role(s)： Peer review

    2021

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    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：12

  • B会場責任者

    日本歯科保存学会秋季学術大会  （ 福岡国際会議場 ） 2019.11

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    Type：Competition, symposium, etc. 

Research Projects

  • 歯根膜幹細胞転換を応用した革新的歯周組織再生法の創出

    Grant number：24K12948  2024.4 - 2027.3

    科学研究費助成事業  基盤研究(C)

    長谷川 大学, 前田 英史, 杉井 英樹, 小幡 純子, 糸山 知宏, 兼子 大志

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    Grant type：Scientific research funding

    本研究では、MESTの発現を上昇させる“化合物”を探索し、その化合物を用いた新たな歯根膜幹細胞転換法、およびその化合物の直接応用による“効率的で簡便、かつ安全な歯周組織再生法”の確立を目指す。まず、オミックスデータ解析により歯根膜幹細胞転換を誘導する化合物を同定し、次にその化合物により誘導された歯根膜幹細胞様細胞の歯周組織再生能についてラット歯周組織欠損モデルを用いて評価する。さらに、この化合物の組織欠損部への直接応用が歯周組織再生に及ぼす影響についても検討を行う。

    CiNii Research

  • 歯周組織発生過程の模倣を基盤とするハイブリッド構造体の創成と歯周組織再生への応用

    Grant number：23K24529  2024.4 - 2026.3

    科学研究費助成事業  基盤研究(B)

    友清 淳, 前田 英史, 糸山 知宏, 和田 尚久, 小幡 純子, 杉井 英樹

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    Grant type：Scientific research funding

    歯周組織は神経堤細胞 (NC) に発生を由来することから、NCは歯周組織の発生過程を模倣する上で極めて重要な細胞であると考えられる。申請者らは人工多能性幹細胞 (iPSC) からNC様細胞 (iPSC-NC) への分化誘導法、およびiPSC-NCから歯根膜幹細胞様細胞 (iPSC-PDLSC) への分化誘導法を確立している。そこで本研究では、これらの細胞に対するゲノム解析結果から同定した、iPSC-NCからiPSC-PDLSCへの分化を誘導する形態形成因子および足場材について至適化を行い、その結果を基にiPSC-NC/形態形成因子/足場材から構成されるハイブリッド構造体を作製する。

    CiNii Research

  • To elucidate the mechanism of senescence of dentin-pulp complex targeting the oxidative stress.

    Grant number：23K24528  2024.4 - 2026.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Grant type：Scientific research funding

    CiNii Research

  • ナノハイドロキシアパタイト含有レジンを応用した歯周組織再生誘導型歯質接着材の開発

    Grant number：23K09171  2023 - 2025

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    杉井 英樹, 前田 英史, 友清 淳, 長谷川 大学, 濱野 さゆり, 糸山 知宏, 兼子 大志, 藤野 翔香, 小幡 純子

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    Authorship：Principal investigator  Grant type：Scientific research funding

    naHAp/SBがセメント質・歯根膜誘導型の新規材料として、破折歯の治療に応用できるとの仮説を立て、naHAp/SBを破折歯の治療に応用し、naHAp/SBのセメント質・歯根膜再生能を明らかにすることを計画した。まず、in vitroにて、歯根膜幹細胞（PDLSCs）を用いて、naHAp/SBが、その歯質接着能、生体親和性、機械的強度、および生体活性（セメント質および歯根膜形成誘導能）に及ぼす影響について解析を行う。その後、in vivoにて、当研究室で樹立したラット歯周組織欠損モデルを応用し、ラット臼歯部歯根破折歯モデルを作製する。同動物モデルを用いて、破折部周囲の組織の治癒状態を検証する。

    CiNii Research

  • 歯周組織発生過程の模倣を基盤とするハイブリッド構造体の創成と歯周組織再生への応用

    Grant number：22H03271  2022 - 2023

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    友清 淳, 前田 英史, 糸山 知宏, 和田 尚久, 小幡 純子, 杉井 英樹, 藤野 翔香

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    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

    iPSC-NCをPDL-ECM上で培養することにより、PDLSCの表現型を示すiPSC-PDLSCへと分化誘導する方法を明らかにし、iPSC-NCからiPSC-PDLSCへの分化誘導には、cWNTシグナルの抑制およびHAシグナルの活性化が重要であることを特定し、これらの標的分子であるNRXならびにHAが、NC細胞株のPDLSC様細胞分化を促進することを明らかにした。本研究では、これらの研究成果を基にiPSC-NC/opt-NRX/8 x 105 Da HAから構成されるハイブリッド構造体を作製し、それらを移植することで、歯周組織発生の模倣を介して歯周組織の再生を誘導する計画である。

    CiNii Research

  • To elucidate the mechanism of senescence of dentin-pulp complex targeting the oxidative stress.

    Grant number：22H03270  2022 - 2023

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

    CiNii Research

  • ナノハイドロキシアパタイト含有レジンを応用した歯周組織再生誘導型歯質接着材の開発

    2021.4 - 2024.3

    　 More details

    Authorship：Principal investigator 

  • Decorinの組織再生賦活化特性を応用した新規歯周組織再生療法の開発

    2021.4 - 2023.3

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    Authorship：Principal investigator 

  • 転写因子PAX9を標的とした歯根膜幹細胞誘導因子の同定

    Grant number：21K09876  2021 - 2024

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    濱野 さゆり, 前田 英史, 吉田 晋一郎, 糸山 知宏, 藤野 翔香, 小幡 純子, 杉井 英樹

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    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

    HPDLCが産生する多数の細胞外基質から、歯根膜幹細胞誘導因子を同定することは困難である。そこで申請書らは、歯根膜幹細胞誘導因子の産生を制御する転写因子に着目し、HPDLCにおける遺伝子発現の網羅的解析を用いてPAX9を抽出した。本研究では、PAX9が歯根膜幹細胞誘導に及ぼす影響について検討し、その下流にある歯根膜幹細胞誘導因子を同定することを目的とした。本研究は、iPS細胞から多量の歯根膜幹細胞の獲得する方法を明らかにし、それを応用した新規歯周組織再生療法の開発研究に繋がるものである。

    CiNii Research

  • Development of a new drug for periodontal regeneration based on the activation of neural crest cells

    Grant number：21K19608  2021 - 2024

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Challenging Research(Exploratory)

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    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

    CiNii Research

  • 根面う蝕を標的としたファージ療法の創出

    Grant number：21K09918  2021 - 2024

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    小幡 純子, 前田 英史, 友清 淳, 濱野 さゆり, 杉井 英樹

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    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

    今後も増え続ける高齢者の健康維持という課題に対して、根面う蝕は歯科界において解決しなければならない問題である。現在、根面う蝕に対する治療法としてフッ化物の応用が推奨されているが、この方法はう蝕原性細菌に対しては酸産生の抑制を期待するという間接的なものでしかなく、積極的な効果は望めない。そこで、近年臨床応用の研究が進んでいるバクテリオファージを利用した「ファージ療法」に着目した。本研究では、根面う蝕細菌に対して特異的に作用するバクテリオファージを探索・同定し、このバクテリオファージに標的細菌を死滅させることにより根面う蝕の進行を停止させるという新たな治療法を確立することを目的とする。

    CiNii Research

  • Development of new periodontal regeneration therapy using Decorin

    Grant number：21K16994  2021 - 2023

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Early-Career Scientists

    Sugii Hideki

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    Authorship：Principal investigator  Grant type：Scientific research funding

    The aim of this study is to clarify the biological functions of decorin (DCN) in the healing and regeneration of wounded periodontal tissue. We investigated the expression pattern of DCN during the healing of wounded periodontal tissue in rats and the effects of DCN on the osteoblastic differentiation of human periodontal ligament (PDL) stem cells (HPDLSCs). The expression of DCN was increased around the wounded PDL tissue on day 5 after surgery. Furthermore, DCN promoted the osteoblastic differentiation of HPDLSCs. DCN did not affect proliferation, migration, or the PDL-related gene expression of HPDLSCs. In conclusion, this study demonstrates that DCN has a role in the healing of wounded periodontal tissue. Furthermore, DCN secreted from PDL cells may contribute to bone healing by upregulating osteoblastic differentiation in HPDLSCs, indicating a therapeutic effect of DCN in periodontal tissue regeneration.

    CiNii Research

  • 歯根膜幹細胞由来エクソソームを用いた歯周組織再生療法の開発

    2019.4 - 2024.3

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    Authorship：Principal investigator 

  • Activin Aの二極性細胞分化誘導特性を基盤とした新規歯周組織再生療法の開発

    2019.4 - 2021.3

    　 More details

    Authorship：Principal investigator 

  • Activin Aの二極性細胞分化誘導特性を基盤とした新規歯周組織再生療法の開発

    Grant number：19K19001  2019 - 2020

    日本学術振興会  科学研究費助成事業  若手研究

    　 More details

    Authorship：Principal investigator  Grant type：Scientific research funding

  • iPS創薬とゲノム創薬の融合による歯根膜幹細胞賦活化物質の創出と再生医療への応用

    Grant number：18K19651  2018 - 2021

    日本学術振興会  科学研究費助成事業  挑戦的研究（萌芽）

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    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

  • Activin Aの選択的分化誘導能を応用した新規歯周組織再生療法の開発

    2017.4 - 2019.3

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    Authorship：Principal investigator 

  • iPS細胞を用いた3次元的歯根膜作製法の確立と人工歯根開発への応用

    Grant number：17H01598  2017 - 2022

    日本学術振興会  科学研究費助成事業  基盤研究(A)

    前田 英史, 友清 淳, 吉田 晋一郎, 濱野 さゆり, 和田 尚久, 杉井 英樹, 長谷川 大学

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    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

    iPS細胞から歯根膜幹細胞様細胞（iPDLSC）への分化誘導法を明らかにし、分化に関わる因子として、転写因子PAX9およびFibrillin 2を検出した。また、ヒト歯根膜幹細胞株から作製したスフェロイドを、バイオ3Dプリンターを用いてチューブ状の構造体を作製した。この構造体は、ハイドロキシアパタイト(HAp)棒を内部に挿入することで、歯根膜様の特性の獲得に加えてセメント質、血管および骨の特性を示すことを確認した。そこで、iPDLSCのスフェロイドを用いて同様にチューブ状構造体を作製し、HAp棒を挿入して生体内に移植を行い、骨への生着を確認した。

    CiNii Research

  • iPS由来神経堤細胞によるバイオミメティクスに基づいた新規歯周組織再生療法の開発

    Grant number：17H04385  2017 - 2021

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

  • Activin Aの選択的分化誘導能を応用した新規歯周組織再生療法の開発

    Grant number：17H06945  2017 - 2018

    科学研究費助成事業  若手研究(スタートアップ)

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    Authorship：Principal investigator  Grant type：Scientific research funding

  • Dlx5が軟口蓋の筋肉の発達に及ぼす影響について International coauthorship

    2016.4 - 2017.11

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    Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid) 

    口蓋裂は出生時に生じる病変であるが、そのうち硬口蓋と軟口蓋に分類され、硬口蓋に関する研究報告は多数存在するが、軟口蓋に関する研究はほとんど存在しない。そこで、本プロジェクトでは、軟口蓋裂に着目し研究を行うこととした。今回、その中でも過去の報告より、Dlx5欠損マウスにおいて軟口蓋の短小化を引き起こすとの報告より、Dlx5の軟口蓋の筋の発達機構に及ぼす影響について解析することにより、軟口蓋裂の発生機序の解明を行うことを目標とした。

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Class subject

  • 歯科保存学

    2024.4 - 2024.9   First semester

  • 学生臨床実習および臨床予備実習

    2023.4 - 2024.3   Full year

  • 歯科保存学

    2023.4 - 2023.9   First semester

  • 臨床実習および臨床予備実習

    2022.4 - 2023.3   Full year

  • 歯科保存学

    2022.4 - 2022.9   First semester

  • 臨床実習および臨床予備実習

    2021.4 - 2022.3   Full year

  • 臨床実習および臨床予備実習

    2020.4 - 2021.3   Full year

  • 臨床実習および臨床予備実習

    2019.4 - 2020.3   Full year

  • 臨床実習および臨床予備実習

    2018.4 - 2019.3   Full year

  • リサーチエクスポージャーおよびアーリーエクスポージャー

    2018.4 - 2019.3   Full year

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FD Participation

  • 2024.9   Role：Participation   Title：増える認知症と対策（福岡市）

    Organizer：University-wide

  • 2024.7   Role：Participation   Title：入学者選抜における面接試験～位置付けと評価方法について

    Organizer：Undergraduate school department

  • 2024.6   Role：Participation   Title：本学の国家試験への取り組み

    Organizer：Undergraduate school department

  • 2023.6   Role：Participation   Title：「科学研究費補助金採択率向上に向けた工夫」

    Organizer：Undergraduate school department

  • 2022.4   Role：Participation   Title：2022年度臨床実地試験（CPX）について

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2022.2   Role：Participation   Title：令和３年度馬出地区４部局合同男女共同参画FD

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2021.4   Role：Participation   Title：CBT問題作問に関するFD

    Organizer：Undergraduate school department

  • 2020.11   Role：Participation   Title：令和２年度馬出地区４部局合同男女共同参画FD

    Organizer：University-wide

  • 2019.9   Role：Participation   Title：科研費申請のススメ！～科学研究費補助金制度と研究計画調書作成時の注意点～

    Organizer：Undergraduate school department

  • 2019.5   Role：Participation   Title：一斉技能試験トライアル教員ＦＤ

    Organizer：Undergraduate school department

  • 2019.3   Role：Participation   Title：2019年度臨床実地試験トライアル説明会（FD）

    Organizer：Undergraduate school department

  • 2018.8   Role：Participation   Title：歯学研究院の研究力分析と研究力強化に向けたScopus/Scival/Pure活用例

    Organizer：Undergraduate school department

  • 2018.5   Role：Participation   Title：歯科学生の臨床実習特定技能試験について

    Organizer：Undergraduate school department

  • 2017.11   Role：Participation   Title：ARO次世代医療センターの活用方法

    Organizer：[Undergraduate school/graduate school/graduate faculty]

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Participation in international educational events, etc.

  • 2022.1

    九州大学病院臨床教育研修センター

    全人的医療人育成教育プログラム（臨床指導者コース）ワークショップ

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    Number of participants：24

  • 2016.2

    頭脳循環を加速する戦略的国際研究ネットワーク推進プログラム

    頭脳循環Kick Off Symposium

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    Venue：日本・福岡

Other educational activity and Special note

  • 2023  Special Affairs 

Outline of Social Contribution and International Cooperation activities

  • 留学中のシリア人留学生に対して、マイクロスコープやMTAなどの最先端の専門的器具に関して、その使用方法および用途について説明を行っている。また、CBCTを用いて、症例およびその治療計画に関して意見を交換した。また、研究内容に関する指導も行っている。

Social Activities

  • 不明

    日本歯科評論2020年増刊  2020.7

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    Audience： General,　Scientific,　Company,　Civic organization,　Governmental agency

    Type：Other

Acceptance of Foreign Researchers, etc.

  • 九州大学大学院歯科保存学研究分野

    Acceptance period： 2018.4 - 2022.3   （Period）：1 month or more

    Nationality：Syrian Arab Republic

Travel Abroad

  • 2016.4 - 2017.5

    Staying countory name 1：United States   Staying institution name 1：University of Southern California

Specialized clinical area

  • Biology / Medicine, Dentistry and Pharmacy / Dentistry / Conservative Dentistry

Clinician qualification

  • Certifying physician

    日本歯科保存学会

Year of medical license acquisition

  • 2011

Notable Clinical Activities

  • 難治性の根尖性歯周炎の症例に対して、マイクロスコープ、拡大鏡を用いて肉眼では発見できない根管や破折線などを検知し、CBCTを用いて３次元的に病巣とその原因歯の状態を把握することにより、精度の高い歯内治療を行っている。予後の悪い症例に対しては、歯根端切除術や、意図的再植などの外科処置を行うことにより、非外科治療では治癒が困難であった根尖性歯周炎の治癒を図っている。また、 MTA、レジン系およびバイオガラス系シーラー、ファイバーポストなどの材料を症例に応じて選択して用いることで、歯の長期保存を目指した治療に努めている。さらに昨年、NiTiファイルに関するハンズオンセミナーにスタッフとして参加し、専門的技術の普及に携わった。