An eco-friendly method using deep eutectic solvents immobilized in a microcrystal cellulose-polyvinyl alcohol sponge for parabens analysis in food sample Reviewed International coauthorship International journal

Yusakul G., Thammakhet-Buranachai C., Poorahong S., Sakamoto S., Makkliang F.

Microchemical Journal   191   108758   2023.8

Bioimprinting as a receptor for detection of kwakhurin Reviewed International coauthorship International journal

Sakamoto S., Minami K., Nuntawong P., Yusakul G., Putalun W., Tanaka H., Fujii S., Morimoto S.

Biomolecules   12 ( 8 )   1064   2022.10

Extraction of curcuminoids and ar-turmerone from turmeric (Curcuma longa L.) using hydrophobic deep eutectic solvents (HDESs) and application as HDES-based microemulsions Reviewed International coauthorship International journal

Kongpol K., Sermkaew N., Makkliange F., Khongphan S., Chuaboon L., Sakdamas A., Sakamoto S., Putalun W., Yusakul G.

Food Chemistry   396   133728   2022.10

Harringtonine ester derivatives with enhanced antiproliferative activities against HL-60 and Hela cells Reviewed International coauthorship International journal

Ochi A., Yoritate M., Miyamoto T., Usui K., Yusakul G., Putalun W., Tanaka H., Hirai G., Morimoto S., Sakamoto S.

Journal of Natural Products   85 ( 2 )   345 - 351   2022.5

Transformation of Pueraria candollei var. mirifica phytoestrogens using immobilized and free β-glucosidase, a technique for enhancing estrogenic activity Reviewed International coauthorship International journal

Makkliang F., Juengsanguanpornsuk W., Phaisan S., Sakdamas A., Putalun W., Sakamoto S., Yusakul G.

RSC Advances   11   32067 - 32076   2021.11

Open sandwich fluorescence-linked immunosorbent assay for detection of soy isoflavone glycosides Reviewed International journal

Sakamoto S., Uchiyama H., Yusakul G., Kyokong N., Pongkitwitoon B., Putalun W., Tanaka H., Morimoto S.

Food Chemistry   361   129829   2021.11

Biocompatible natural deep eutectic solvent-based extraction and cellulolytic enzymes mediated transformation of Pueraria mirifica isoflavones, a sustainable approach for increasing health-bioactive constituents Reviewed International journal

Makkliang F., Siriwarin B., Yusakul G., Phaisan S., Sakdamas A., Chuphol N., Putalun W., Sakamoto S.

Bioresources and Bioprocessing   8 ( 1 )   76   2021.8

Molecular cloning and characterization of type III polyketide synthase from Plumbago zeylanica Reviewed International coauthorship International journal

Sakamoto S.*, Morita Y.*, Yusakul G., Putalun W., Tanaka H., Morimoto S.

Journanl of Asian Natural Products Research   23 ( 5 )   478 - 490   2021.5

Plant-made antibody against miroestrol: A new platform for expression of full-length immunoglobulin G against small-molecule targets in immunoassays Reviewed International journal

Rattanapisit K., Kitisripanya T., Konyanee A., Sae-Foo W., Burapapiruin A., Putalun W., Sakamoto S., Phoolcharoen W., Yusakul G.

Plant Cell Reports   40 ( 4 )   723 - 733   2021.4

The colloidal gold nanoparticle based-lateral flow immunoassay for fast and simple detection of plant-derived doping agent, higenamine Reviewed International journal

Nuntawong P., Ochi A., Chaingam J., Tanaka H., Sakamoto S., Morimoto S.

Drug Testing and Analysis   13 ( 4 )   762 - 769   2021.4

Development of a colorless Centella asiatica extract using a natural deep eutectic solvent (NADES) and microwave-assisted extraction (MAE) optimized by response surface methodology Reviewed International journal

Phaisan S., Makkliang F., Putalun W., Sakamoto S., Yusakul G.

RSC Advances   11   8741 - 8750   2021.3

Kwakhurin-magnetic particles conjugates enabled fast enzyme immunoassay for the detection of kwakhurin in Pueraria candollei Reviewed International journal

Sakamoto S., Eto R., Nuntawong P., Yusakul G., Juengwatanatrakul T., Putalun W., Fujii S., Tanaka H., Morimoto S.

Phytochemical Analysis   31 ( 6 )   930 - 936   2020.12

ELISA for the detection of the prohibited doping agent higenamine in medicinal plants Reviewed International journal

Nuntawong P., Tanaka H., Sakamoto S., Morimoto S.

Planta Medica   86 ( 11 )   760 - 766   2020.11

Magnetic particles-based enzyme immunoassay for rapid determination of secoiridoid glycoside, amarogentin Reviewed International journal

Sakamoto S., Wada S., Morita Y., Yamaguchi T., Tanaka H., Morimoto S.

Talanta   194   731 - 736   2019.3

Sensitive quantitative analysis of the bitter glycoside, amarogentin by specific monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay Reviewed International journal

Sakamoto S., Wada S., Tanaka H., Morimoto S.

RSC Advances   8 ( 31 )   17410 - 17416   2018.5

Sodium-periodate mediated harringtonine derivatives and their antiproliferative activity against HL-60 acute leukemia cells Reviewed International journal

Sakamoto S., Miyamoto T., Usui K, Tanaka H., Morimoto S.

Journal of Natural Products   81 ( 1 )   34 - 40   2018.1

Ultrasensitive immunoassay for monocrotaline using monoclonal antibody produced by N, N’-carbonyldiimidazole mediated hapten-carrier protein conjugates Reviewed International journal

Sakamoto S., Nagamitsu R., Yusakul G., Miyamoto T., Tanaka H., Morimoto S.

Talanta   168   67 - 72   2017.5

Sodium periodate-mediated conjugation of harringtonine enabling the production of a highly specific monoclonal antibody, and the development of a sensitive quantitative analysis method Reviewed International journal

Sakamoto S., Yusakul G., Tsuneura Y., Putalun W., Usui K., Miyamoto T., Tanaka H., Morimoto S.

Analyst   142 ( 7 )   1140 - 1148   2017.4

Development of an indirect competitive immunochromatographic strip test for rapid detection and determination of anticancer drug, harringtonine Reviewed International journal

Sakamoto S., Yusakul G., Nuntawong P., Kitisripanya T., Putalun W., Miyamoto T., Tanaka H., Morimoto S.

Journal of Chromatography B   1048   150 - 154   2017.3

Colloidal gold-based indirect competitive immunochromatographic assay for rapid detection of bioactive isoflavone glycosides daidzin and genistin in soy products Reviewed International journal

Sakamoto S., Yusakul G., Pongkitwitoon B., Tanaka H., Morimoto S.

Food Chemistry   194   191 - 195   2016.3

Effect of glycyrrhetinic acid on lipid raft model at the air/water interface Reviewed International journal

Sakamoto S., Uto T., Shoyama Y.

Biochimica et Biophysica Acta   1848 ( 2 )   434 - 443   2015.2

Simultaneous determination of soy isoflavone glycosides, daidzin and genistin by monoclonal antibody-based highly sensitive indirect competitive enzyme-linked immunosorbent assay Reviewed International journal

Sakamoto S., Yusakul G., Pongkitwitoon B., Paudel M.K., Tanaka H., Morimoto S.

Food Chemistry   169   127 - 133   2015.2

Investigation of interfacial behavior of glycyrrhizin with lipid raft model via a Langmuir monolayer study Reviewed International journal

Sakamoto S., Nakahara H., Uto T., Shoyama Y., Shibata O.

Biochimica et Biophysica Acta   1828 ( 4 )   1271 - 1283   2013.4

Modulation of plumbagin production in Plumbago zeylanica using a single-chain variable fragment antibody against plumbagin Reviewed International journal

Sakamoto S., Putalun W., Pongkitwitoon B., Juengwatanatrakul T., Shoyama Y., Tanaka H., Morimoto S.

Plant Cell Reports   31 ( 1 )   103 - 110   2012.1

A fluorescent single domain antibody against plumbagin expressed in silkworm larvae for fluorescence-linked immunosorbent assay (FLISA) Reviewed International journal

Sakamoto S., Pongkitwitoon B., Sasaki-Tabata K., Putalun W., Maenaka K., Tanaka H., Morimoto S.

Analyst   136 ( 10 )   2056 - 2063   2011.5

Efficient silkworm expression of single-chain variable fragment antibody against ginsenoside Re using Bombyx mori nucleopolyhedrovirus bacmid DNA system and its application in enzyme-linked immunosorbent assay for quality control of total ginsenosides Reviewed International journal

Sakamoto S., Pongkitwitoon B., Nakamura S., Maenaka K., Tanaka H., Morimoto S.

Journal of Biochemistry   148 ( 3 )   335 - 340   2010.9

Development of sensitivity-improved fluorescence-linked immunosorbent assay using a fluorescent single-domain antibody against the bioactive naphthoquinone, plumbagin Reviewed International journal

Sakamoto S., Taura F., Pongkitwitoon B., Putalun W., Tsuchihashi R., Kinjo J., Tanaka H., Morimoto S.

Analytical and Bioanalytical Chemistry   396 ( 8 )   2955 - 2963   2010.4

Construction and expression of specificity-improved single-chain variable fragments against the bioactive naphthoquinone, plumbagin Reviewed International journal

Sakamoto S., Taura F., Putalun W., Pongkitwitoon B., Tsuchihashi R., Morimoto S., Tanaka H., Kinjo J., Shoyama Y.

Biological and Pharmaceutical Bulletin   32 ( 3 )   434 - 439   2009.3

Development of an enzyme-linked immunosorbent assay (ELISA) using highly-specific monoclonal antibodies against plumbagin Reviewed International journal

Sakamoto S., Putalun W., Tsuchihashi R., Morimoto S., Kinjo J., Tanaka H.

Analytica Chimica Acta   607 ( 1 )   100 - 105   2008.1

Advanced quality assessment of Sanshishi (Gardenia jasminoides Ellis) and Kampo medicines using a monoclonal antibody against geniposide. Reviewed International journal

Noguchi K., Imahori D., Nishiura R., Nuntawong P., Putalun W., Tanaka H., Morimoto S., Sakamoto S.*

Fitoterapia   174   105829   2024.4

Immunoaffinity separation of miroestrol and deoxymiroestrol from Pueraria candollei var. mirifica (Airy Shaw & Suvat.) Niyomdham using fragment antigen-binding antibody produced via Escherichia coli Reviewed International journal

Juengsanguanpornsuk W., Poopanee N., Krittanai S., Sakamoto S., Tanaka H., Putalun W., Yusakul G.

Phytochemical Analysis   34 ( 6 )   632 - 640   2023.8

Construction of a monoclonal antibody against glabridin (2G4) and development of an enzyme-linked immunosorbent assay Reviewed International journal

Krittanai S., Choonong R., Butdapheng K., Jabsanthia J., Sakamoto S., Putalun W.

Phytochemical Analysis   34 ( 5 )   571 - 579   2023.7

Immunochromatographic assay for miroestrol and deoxymiroestrol, its cross-reactivity, and application in Pueraria mirifica (white Kwao Krua) analysis Reviewed International journal

Chuphol N., Nokkaew N., Makkliang F., Sae-Foo W., Phaisan S., Putalun W., Sakamoto S., Yusakul G.

Phytochemical Analysis   34 ( 4 )   421 - 430   2023.6

Comparative stability and analytical performance of anti-miroestrol recombinant antibody in different cassettes Reviewed International journal

Sae-Foo W., Yusakul G., Kitisripanya T., Nuntawong P., Sakamoto S., Putalun W.

Applied Microbiology and Biotechnology   107 ( 9 )   2887 - 2896   2023.5

Analysis of canthin-6-one alkaloids derived from Eurycoma spp. by micellar liquid chromatography and conventional high-performance liquid chromatography: a comparative evaluation Reviewed International journal

Sakdamas A., Makkliang F., Putalun W., Juengwatanatrakul T., Kanchanapoom T., Sakamoto S., Yusakul G.

RSC Advances   13 ( 9 )   6317 - 6326   2023.2

Improvement in the binding specificity of anti-isomiroestrol antibodies by expression as fragments under oxidizing conditions inside the SHuffle T7 E. coli cytoplasm Reviewed International journal

Juengsanguanpornsuk W., Kitisripanya T., Boonsnongcheep P., Yusakul G., Srisongkram T., Sakamoto S., Putalun W.

Bioscience, Biochemistry, and Biotechnology   86 ( 10 )   1368 - 1377   2022.10

Activated carbon-based immunochromatographic strip test for the rapid qualitative analysis of swertiamarin and sweroside Reviewed International journal

Nuntawong P., Horikawa T., Tanaka H., Morimoto S., Sakamoto S.

Journal of AOAC International   105 ( 5 )   1460 - 1467   2022.9

Monoclonal antibody-based immunoassay for specific quantification of licochalcone A: an active chalcone in licorice Reviewed International journal

Krittanai S., Pichetpongtorn P., Sakamoto S., Putalun W.

Food and Agricultural Immunology   33 ( 1 )   220 - 234   2022.4

Enzyme-linked immunosorbent assay using fragment antigen-binding (Fab) antibody for determination of deoxymiroestrol, a potent phytoestrogen from Pueraria candollei Reviewed International journal

Sae-Foo W., Krittanai S., Juengsanguanpornsuk W., Yusakul G., Kitisripanya T., Sakamoto S., Putalun W.

Food and Agricultural Immunology   32 ( 1 )   336 - 348   2021.7

Fragment antigen-binding (Fab) antibody-based lateral flow immunoassay for rapid and sensitive detection of potent phytoestrogen, deoxymiroestrol Reviewed International journal

Sae-Foo W., Krittanai S., Juengsanguanpornsuk W., Yusakul G., Sakamoto S., Putalun W.

Journal of Natural Medicines   75 ( 4 )   1043 - 1049   2021.7

Immunochromatographic assay for the detection of kwakhurin and its application for identification of Pueraria candollei Wall. ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham Reviewed International journal

Phaisan S., Yusakul G., Nuntawong P., Sakamoto S., Putalun W., Morimoto S., Tanaka H.

Phytochemical Analysis   32   503 - 511   2021.7

Enhancement of nutraceutical property in licorice callus cultures using sample pretreatment strategy Reviewed International journal

Krittanai S., Buraphaka H., Sakamoto S., Putalun W.

International Journal of Food Science and Technology   56 ( 11 )   5843 - 5852   2021.6

Rapid magnetic particles-based enzyme immunoassay for the quality control of Glycyrrhiza spp. based on glycyrrhizin content Reviewed International journal

Minami K., Yusakul G., Fujii S., Putalun W., Tanaka H., Sakamoto S., Morimoto S.

Fitoterapia   148   104794   2021.1

Competitive immunochromatographic test strips for the rapid semi-quantitative analysis of the biologically active bitter glycoside, amarogentin Reviewed International journal

Nuntawong P.*, Lohseethong K.*, Juengwatanatrakul T., Yusakul G., Putalun W., Tanaka H., Sakamoto S., Morimoto S.

Journal of Immunoassay and Immunochemistry   42 ( 1 )   48 - 61   2021.1

Quantification of methylisomiroestrol, a phytoestrogen of Pueraria candollei, by enzyme-linked immunosorbent assay in comparison with a high-performance liquid chromatography Reviewed International journal

Juengsanguanpornsuk W., Yusakul G., Kitisripanya T., Krittanaia S., Juengwatanatrakul T., Sakamoto S., Putalun W.

Journal of Pharmaceutical Biomedical Analysis   192   113674   2021.1

A monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay to quantify swertiamarin and its related compounds in Swertia japonica Makino Reviewed International journal

Nuntawong P.*, Horikawa T.*, Ochi A., Wada S., Tsuneura Y., Tanaka H., Sakamoto S., Morimoto S.

Phytochemical Analysis   32   512 - 520   2020.7

Expression of actively soluble antigen‑binding fragment (Fab) antibody and GFP fused Fab in the cytoplasm of the engineered Escherichia coli Reviewed International coauthorship International journal

Krittanai S., Putalun W., Sakamoto S., Tanaka H., Juengwatanatrakul T., Yusakul G.

Molecular Biology Reports   47 ( 6 )   4519 - 4529   2020.4

A green and effective method using oils to remove chlorophyll from Chromolaena odorata (L.) R.M. King & H. Rob Reviewed International journal

Phaisan S., Yusakul G., Sakdamas A., Taluengjit N., Sakamoto S., Putalun W.

Songklanakarin Journal of Science and Technology   42 ( 5 )   1084 - 1090   2020.4

Honey as a solvent for the green extraction, analysis, and bio-conversion of daidzin from Pueraria candollei var. mirifica root Reviewed International journal

Phaisan S., Yusakul G., Nuntawong P., Sakamoto S., Putalun W., Morimoto S., Tanaka H.

Pharmacognosy Magazine   16 ( 71 )   524 - 530   2020.4

An estimated quantitative lateral flow immunoassay for determination of artesunate using monoclonal antibody Reviewed International journal

Kitisripanya T., Sermpradit W., Sakamoto S., Tanaka H., Putalun W.

Biomedical Chromatography   34 ( 2 )   e4718   2020.2

Preincubation format for a sensitive immunochromatographic assay for monocrotaline, a toxic pyrrolizidine alkaloid Reviewed International journal

Yusakul G., Sakamoto S., Chanpokapaiboon K., Tanaka H., Morimoto S.

Phytochemical Analysis   30 ( 6 )   653 - 660   2019.12

Preparation of a highly specific single chain variable fragment antibody targeting miroestrol and its application in quality control of Pueraria candollei by enzyme-linked immunosorbent assay Reviewed International journal

Pongkitwitoon B., Boonsnongcheep P., Kitisripanya T., Yusakul G., Sakamoto S., Tanaka H., Morimoto S., Putalun, W.

Phytochemical Analysis   30 ( 6 )   600 - 608   2019.12

Development of a simple and rapid method for detection of isomiroestrol in Pueraria candollei by an immunochromatographic strip test Reviewed International journal

Chaingam J., Kitisripanya T., Krittanai S., Sakamoto S., Tanaka H., Putalun W.

Journal of Natural Medicines   73 ( 3 )   577 - 583   2019.9

Modification of first constant domain of heavy chain (CH1) enabled effective folding of functional anti-forskolin antigen-binding fragment (Fab) for sensitive quantitative analysis Reviewed International journal

Yusakul G., Sakamoto S., Tanaka H., Morimoto S.

Biotechnology Progress   35 ( 4 )   e2822   2019.7

Selective and reversible modification of kinase cysteines with chlorofluoroacetamides Reviewed International journal

Shindo N., Fuchida H., Sato M., Watari K., Shibata T., Kuwata K., Miura C., Okamoto K., Hatsuyama Y., Tokunaga K., Sakamoto S., Morimoto S., Abe Y., Shiroishi M., Caaveiro J.M.M., Ueda T., Tamura T., Matsunaga N., Nakao T., Koyanagi S., Ohdo S., Yamaguchi Y. Hamachi I., Ono M., Ojida A.

Nature Chemical Biology   15 ( 3 )   250 - 258   2019.3

An indirect competitive enzyme-linked immunosorbent assay toward standardization of Pueraria candollei based on its unique isoflavonoid, kwakhurin Reviewed International journal

Yusakul G.*, Togita R.*, Minami K.*, Chanpokapaiboon K., Juengwatanatrakul T., Putalun W., Tanaka H., Sakamoto S., Morimoto S.

Fitoterapia   133   23 - 28   2019.3

A recombinant Fab antibody against kwakhurin as a tool for sensitive indirect competitive ELISA Reviewed International journal

Chanpokapaiboon K., Khoonrit P., Yusakul G., Juengwatanatrakul T., Putalun W., Tanaka H., Sakamoto S., Morimoto S.

Current Pharmaceutical Biotechnology   19 ( 14 )   1170 - 1176   2018.12

Improvement of heavy and light chain assembly by modification of heavy chain constant region 1 (CH1): Application for construction of anti-paclitaxel fragment antigen-binding (Fab) Reviewed International journal

Yusakul G., Sakamoto S., Tanaka H., Morimoto S.

Journal of Biotechnology   288   41 - 47   2018.12

Development of a colloidal gold nanoparticle-based immunochromatographic strip for the one-step detection of miroestrol and puerarin Reviewed International journal

Krittanai S., Kitisripanya T., Udomsin O., Tanaka H., Sakamoto S., Juengwatanatrakul T. Putalun W.

Biomedical Chromatography   32 ( 11 )   e4330   2018.11

A monoclonal antibody-based enzyme-linked immunosorbent assay for determination of homoharringtonine Reviewed International journal

Pongkitwitoon B.*, Sakamoto S.*, Nagamitsu R., Putalun W., Tanaka H., Morimoto S.

Planta Medica   84 ( 14 )   1038 - 1044   2018.9

Enzyme linked immunosorbent assays for total potent estrogenic miroestrol and deoxymiroestrol of Pueraria candollei, a Thai herb for menopause remedy Reviewed International journal

Yusakul G., Kitisripanya T., Juengwatanatrakul T., Sakamoto S., Tanaka H., Putalun W.

Journal of Natural Medicines   72 ( 3 )   641 - 650   2018.6

Different expression systems resulted in varied binding properties of anti-paclitaxel single chain variable fragment antibody clone 1C2 Reviewed International journal

Yusakul G., Sakamoto S., Nuntawong P., Tanaka H., Morimoto S.

Journal of Natural Medicines   72 ( 1 )   310 - 316   2018.1

Bacterial expressions of single-chain variable fragment (scFv) antibody against ganoderic acid A, a cost-effective approach for its quantitative analysis using the scFv based enzyme-linked immunosorbent assay Reviewed International journal

Yusakul G., Nuntawong P., Sakamoto S., Ratnatilaka Na Bhuket P., Kohno T., Kikkawa N., Rojsitthisak P., Shimizu K., Tanaka H., Morimoto S.

Biological and Pharmaceutical Bulletin   40 ( 10 )   1767 - 1774   2017.10

Development of an immunoassay using an anti-wogonin glucuronide monoclonal antibody Reviewed International journal

Paudel M.K., Sakamoto S., Huy L.V., Tanaka H., Miyamoto T., Akihito T., Morimoto S.

Journal of Immunoassay and Immunochemistry   38 ( 5 )   457 - 470   2017.10

Development of an enzyme-linked immunosorbent assay for determination of miroestrol using an anti-miroestrol monoclonal antibody Reviewed International journal

Kitisripanya T., Krittanai S., Udomsin O., Juthasit K., Komaikul J., Juengwatanatrakul T., Sakamoto S., Tanaka H., Morimoto S., Putalun W.

Planta Medica   83 ( 10 )   855 - 861   2017.10

An overview and comparison of a recombinant antigen-binding fragment and an antigen-binding fragment from a monoclonal antibody against wogonin glucuronide Reviewed International journal

Paudel M.K., Sakamoto S., Morimoto S., Tanaka H.

Journal of Natural Medicines   71 ( 4 )   703 - 710   2017.9

A lateral flow, colloidal gold-based immunoassay for rapid detection of miroestrol in samples of White Kwao Krua, a phytoestrogen-rich plant Reviewed International journal

Kitisripanya T., Inyai C., Komaikul J., Krittanai S., Juengwatanatrakul T., Sakamoto S., Tanaka H., Morimoto S., Putalun W.

Journal of Natural Medicines   71 ( 4 )   659 - 664   2017.9

An immunochromatographic assay for rapid detection of salvinorin A Reviewed International journal

Paudel M.K., Sakamoto S., Shirota O., Morimoto S., Tanaka H.

Journal of Immunoassay and Immunochemistry   38 ( 4 )   438 - 448   2017.6

The effect of varying the peptide linker length in a single chain variable fragment antibody against wogonin glucuronide Reviewed International journal

Paudel M.K., Sakamoto S., Huy L.V., Tanaka H., Miyamoto T., Morimoto S.

Journal of Biotechnology   251   47 - 52   2017.6

Tubulin polymerization stimulating-activity of Ganoderma triterpenoids Reviewed International journal

Kohno T.*, Hai-Bang T.*, Zhu Q., Amen Y.M., Sakamoto S., Tanaka H., Morimoto S., Shimizu K.

Journal of Natural Medicines   71 ( 2 )   457 - 462   2017.1

Immunochromatographic strip assay for detection of bioactive Ganoderma triterpenoid, ganoderic acid A in Ganoderma lingzhi Reviewed International journal

Sakamoto S., Kikkawa N., Kohno T., Shimizu K., Tanaka H., Morimoto S.

Fitoterapia   114   51 - 55   2016.10

Efficient expression of single chain variable fragment antibody against paclitaxel using Bombyx mori nucleopolyhedrovirus bacmid DNA system and its characterizations Reviewed International journal

Yusakul G., Sakamoto S., Tanaka H., Morimoto S.

Journal of Natural Medicines   70 ( 3 )   592 - 601   2016.7

Effect of linker length between variable domains of single chain variable fragment antibody against daidzin on its reactivity Reviewed International journal

Yusakul G., Sakamoto S., Pongkitwitoon B., Tanaka H., Morimoto S.

Bioscience, Biotechnology, and Biochemistry   80 ( 7 )   1306 - 1312   2016.7

Detection of ganoderic acid A in Ganoderma lingzhi by an indirect competitive enzyme-linked immunosorbent assay Reviewed International journal

Sakamoto S.*, Kohno T.*, Shimizu K., Tanaka H., Morimoto S.

Planta Medica   82 ( 8 )   747 - 751   2016.5

A new approach of indirect enzyme-linked immunosorbent assay for determination of D-glutamic acid through in situ conjugation Reviewed International journal

Sakamoto S.*, Nagamitsu R.*, Matsuura Y., Tsuneura Y., Kurose H., Tanaka H., Morimoto S.

Journal of Immunoassay and Immunochemistry   37 ( 3 )   296 - 306   2016.3

Preparation and application of a monoclonal antibody against the isoflavone glycoside daidzin using a Mannich reaction-derived hapten conjugate Reviewed International journal

Yusakul G., Sakamoto S., Juengwatanatrakul T., Putalun W., Tanaka H., Morimoto S.

Phytochemical Analysis   27 ( 1 )   81 - 88   2016.1

Production and characterization of highly-specific monoclonal antibodies to D-glutamic acid Reviewed International journal

Sakamoto S.*, Matsuura Y.*, Yonenaga Y., Tsuneura Y., Aso M., Kurose H., Tanaka H., Morimoto S.

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy   33   414 - 419   2014.12

Miscibility behavior of sphingomyelin with phytosterol derivatives by a Langmuir monolayer approach Reviewed International journal

Sakamoto S., Nakahara H., Shibata O.

Journal of Oleo Science   62 ( 10 )   809 - 824   2013.10

Development of an indirect competitive enzyme-linked immunosorbent assay (icELISA) using highly specific monoclonal antibody against paclitaxel Reviewed International journal

Chao Z., Tan M., Paudel M.K., Sakamoto S., Ma L., Sasaki-Tabata K., Tanaka H., Shoyama Y., Xuan L., Morimoto S.

Journal of Natural Medicines   67 ( 3 )   512 - 518   2013.7

Examination of fluorination effect on physical properties of saturated long-chain alcohols by DSC and Langmuir monolayer Reviewed International journal

Nakahara H., Nakamura S., Okahashi Y., Kitaguchi D., Kawabata N., Sakamoto S., Shibata O.

Colloids and Surfaces B: Biointerfaces   102 ( 2 )   472 - 478   2013.2

Elicitation effect on production of plumbagin in in vitro culture of Drosera indica L. Reviewed International journal

Juengwatanatrakul T., Sakamoto S., Tanaka H., Putalun W.

Journal of Medicinal Plants Research   5 ( 19 )   4949 - 4953   2011.9

An enzyme-linked immunosorbant assay using monoclonal antibody against Bacoside A3 for determination of jujubogenin glycosides in Bacopa monnieri (L.) Wettst Reviewed International journal

Tothiam C., Phrompittayarat W., Putalun W., Tanaka H., Sakamoto S., Khan A.I., Ingkaninan K.

Phytochemical Analysis   22 ( 5 )   385 - 391   2011.9

Construction, expression, and characterization of a single-chain variable fragment antibody against 2, 4- dichlorophenoxyacetic acid in the hemolymph of silkworm larvae Reviewed International journal

Sakamoto S., Pongkitwitoon B., Nakamura S., Sasaki-Tabata K., Tanizaki Y., Maenaka K., Tanaka H., Morimoto S.

Applied Biochemistry and Biotechnology   164 ( 6 )   715 - 728   2011.7

Flavone-catalyzed apotosis in Scutellaria baicalensis Reviewed International journal

Hirunuma M., Shoyama Y., Sasaki K., Sakamoto S., Taura F., Shoyama Y., Tanaka H., Morimoto S.

Phytochemistry   72 ( 8 )   752 - 760   2011.6

A chimera of green fluorescent protein with single chain variable fragment antibody against ginsenosides for fluorescence-linked immunosorbent assay Reviewed International journal

Sakamoto S., Tanizaki Y., Pongkitwitoon B., Tanaka H., Morimoto S.

Protein Expression and Purification   77 ( 1 )   124 - 130   2011.5

Development of an enzyme-linked immunosorbent assay to determine puerarin and its aglycone daidzein Reviewed International journal

Pongkitwitoon B., Sakamoto S., Tanaka H., Tsuchihashi R., Kinjo J., Morimoto S., Putalun W.

Journal of Natural Medicines   65 ( 1 )   31 - 36   2011.1

Single-chain variable fragment antibody against ginsenoside Re as an effective tool for the determination of ginsenosides in various ginsengs Reviewed International journal

Pongkitwitoon B.*, Sakamoto S.*, Morinaga O., Juengwatanatrakul T., Shoyama Y., Tanaka H., Morimoto S.

Journal of Natural Medicines   65 ( 1 )   24 - 30   2011.1

Expression, purification and characterization of anti-plumbagin single-chain variable fragment antibody in Sf9 insect cell Reviewed International journal

Sakamoto S., Taura F., Tsuchihashi R., Putalun W., Kinjo J., Tanaka H., Morimoto S.

Hybridoma   29 ( 6 )   481 - 488   2010.12

Enhanced plumbagin production from in vitro cultures of Drosera burmanii using elicitation Reviewed International journal

Putalun W., Udomsin O., Yasakul G., Juengwatanatrakul T., Sakamoto S., Tanaka H.

Biotechnology Letters   32 ( 5 )   721 - 724   2010.5

Enzyme-linked immunosorbent assay for total isoflavonoids in Pueraria candollei using anti-puerarin and anti-daidzin polyclonal antibodies Reviewed International coauthorship International journal

Pongkitwitoon B., Sakamoto S., Tanaka H., Tsuchihashi R., Kinjo J., Morimoto S., Putalun W.

Planta Medica   76 ( 8 )   831 - 836   2010.5

Microbial transformation and bioactivation of isoflavones from Pueraria flowers by human intestinal bacterial strains Reviewed International journal

Tsuchihashi R., Kodera M., Sakamoto S., Nakajima Y., Yamazaki T., Niiho Y., Nohara T., Kinjo J.

Journal of Natural Medicines   63 ( 3 )   254 - 260   2009.7

Development of eastern blotting technique for sennoside A and sennoside B using anti-sennoside A and anti-sennoside B monoclonal antibodies Reviewed International journal

Morinaga O., Uto T., Sakamoto S., Putalun W., Lhieochaiphant S., Tanaka H., Shoyama Y.

Phytochemical Analysis   20 ( 2 )   154 - 158   2009.3

Enzyme-linked immunosorbent assay for total sennosides using antisennside A and anti-sennoside B monoclonal antibodies Reviewed International journal

Morinaga O., Uto T., Sakamoto S., Tanaka H., Shoyama Y.

Fitoterapia   80 ( 1 )   28 - 31   2009.1

Microbial metabolism of soy isoflavones by human intestinal bacterial strains Reviewed International journal

Tsuchihashi R., Sakamoto S., Kodera M., Nohara T., Kinjo J.

Journal of Natural Medicines   62 ( 4 )   456 - 460   2008.10

Development of an enzyme linked immunosorbent assay for direct determination of anticancer drug vitamin K3 in serum Reviewed International journal

Sakamoto S., Sakoda J., Morinaga O., Putalun W., Tsuchihashi R., Morimoto S., Kinjo J., Tanaka H.

Journal of Health Science   54 ( 4 )   508 - 511   2008.6

大豆イソフラボン配糖体の検出を目指したオープンサンドイッチ蛍光イムノアッセイの確立

内山　裕貴、坂元　政一、Gorawit Yusakul、Benyakan Pongkitwitoon、Waraporn Putalun、田中　宏幸、森元　聡

日本薬学会第142年会  2022.3 

植物のアサの成分 THCA に対する解毒機構に関する研究

井上　花奈、坂元　政一、森元　聡

日本生薬学会第67回年会  2021.9 

ハリントン誘導体のHL-60とHela細胞に対する増殖阻害活性に関する研究

越智　昭裕、寄立　麻琴、臼井　一晃、宮本　智文、平井　剛、田中　宏幸、坂元　政一、森元　聡

日本生薬学会第67回年会  2021.9 

植物二次代謝産物の免疫化学的分析法の開発 Invited

坂元　政一

日本生薬学会第65回年会  2018.9 

セイロンマツリ由来ポリケタイド合成酵素(polyketide synthase: PKS)に関する研究

森田　優衣、坂元　政一、田中　宏幸、森元　聡

日本生薬学会第65回年会  2018.9 

過ヨウ素酸ナトリウムによるハリントニン誘導体の作出及びヒト前骨髄性白血病細胞HL60に対する増殖阻害活性

坂元　政一、宮本　智文、臼井　一晃、田中　宏幸、森元　聡

日本薬学会第138年会  2018.3 

Cephalotaxus属由来ハリントニンの一本鎖抗体の作製及びその応用研究

小松 頌平, 坂元 政一, Gorawit Yusakul, Warapon Putalun, 田中 宏幸, 森元 聡

日本生薬学会第64回年会  2017.9 

Immunoassays for cephalotaxus alkaloids, harringtonine and antiproliferative activity of its derivatives Invited International conference

Sakamoto S.

5th International Conference on Pharmaceuticals, Nutraceuticals and Cosmetic Science (IPNaCS 2017)  2017.8 

Sodium periodate-mediated conjugation of harringtonine enabling the production of a highly specific monoclonal antibody, and the development of a sensitive quantitative analysis method Invited International conference

Sakamoto S.

International Annual Meeting in Pharmaceutical Sciences (JSPS-NRCT 2017 and IAMPS 33)  2017.3 

A single-chain variable fragment antibody against anti-leukemia agent, harringtonine as a tool for immunomodulation International conference

Komatsu S., Sakamoto S., Yusakul G., Putalun W., Miyamoto T., Tanaka H., Morimoto S.

The 9th Joint Natural Products Conference 2016 in Copenhagen  2016.7 

Development of highly sensitive immunological techniques for determination of cephalotaxus alkaloids, harringtonine International conference

Sakamoto S., Yusakul G., Nagamitsu R., Tsuneura Y., Putalun W., Miyamoto T., Tanaka H., Morimoto S.

The 9th Joint Natural Products Conference 2016 in Copenhagen  2016.7 

Production of highly specific monoclonal antibody against representative cephalotaxine alkaloids, harringtonine and its application to sensitive quantitative/qualitative analyses International conference

Sakamoto S., Yusakul G., Nagamitsu R., Tsuneura Y., Miyamoto T., Tanaka H., Morimoto S.

The 6th International Conference on Natural Products for Health and Beauty (NATPRO 6)  2016.1 

Modulation of plumbagin production in Plumbago zeylanica using a single-chain variable fragment antibody against plumbagin Invited International conference

Sakamoto S., Putalun W., Pongkitwitoon B., Shoyama Y., Tanaka H., Morimoto S.

The 1st International Conference on Herbal and Traditional Medicine (HTM 2015)  2015.1 

スフィンゴミエリンと植物ステロール誘導体との2成分単分子膜挙動

坂元　政一, 中原　広道, 柴田　攻

第64回コロイドおよび界面化学討論会  2013.9 

Miscibility behavior of sphingomyelin with phytosterol derivatives by a Langmuir monolayer approach International conference

Sakamoto S., Nakahara H., Shibata O.

27th Conference of the European Colloid and Interface Society (27th ECIS)  2013.9 

Specific effect of glycyrrhetinic acid on lipid raft model membrane: A Langmuir monolayer study International conference

Sakamoto S., Nakahara H., Shibata O.

27th Conference of the European Colloid and Interface Society (27th ECIS)  2013.9 

脂質ラフト構成分子とステアリルグリチルレチン酸のLangmuir単分子膜法による混和挙動解析

臼井　千尋, 坂元　政一, 中原　広道, 柴田　攻

日本薬学会第133年会  2013.3 

脂質ラフト生体膜モデルにおけるグリチルレチン酸の界面科学的挙動―Langmuir単分子膜―

坂元　政一, 中原　広道, 柴田　攻

日本薬学会第133年会  2013.3 

Interaction of glycyrrhizin with lipid rafts model by Langmuir monolayer study International conference

Sakamoto S., Nakahara H., Shibata O.

26th Conference of the European Colloid and Interface Society (26th ECIS)  2012.9 

Langmuir単分子膜手法を用いた脂質ラフト生体膜モデルにおけるグリチルリチンの界面科学的挙動

坂元　政一, 中原　広道, 柴田　攻

日本薬学会第132年会  2012.3 

Interaction of glycyrrhizin with the ternary mixture of sphingomyelin/DOPC/cholesterol by Langmuir monolayer study International conference

Sakamoto S., Shibata O., Nakahara H.

The 4th Asian Conference on Colloid and Interface Science  2011.11 

抗体を基盤としたプルンバギン定量分析法及びセイロンマツリの分子育種

坂元　政一, Benyakan Pongkitwitoon, 田中　宏幸, 森元　聡, Waraporn Putalun, 柴田　攻

平成23年度物理化学インターカレッジセミナー兼油化学界面科学部会九州地区講演会  2011.11 

抗ジンセノシドRe小型化抗体の作製及び酵素免疫吸着測定法(ELISA)

坂元　政一, Benyakan Pongkitwitoon, 田中　宏幸, 森元　聡, 前仲　勝実, 柴田　攻

第63回コロイドおよび界面化学討論会  2011.9 

Efficient silkworm expression of single-chain variable fragment antibody against ginsenoside Re using Bombyx mori nucleopolyhedrovirus bacmid DNA system and its application in ELISA for quality control of total ginsenosides International conference

Sakamoto S., Pongkitwitoon B., Nakamura S., Maenaka K., Tanaka H., Morimoto S.

58th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research  2010.8 

昆虫細胞(Sf9)発現系を用いた抗plumbagin小型化抗体の発現及びその特徴付け

坂元　政一, 田浦　太志, 土橋　良太, Waraporn Putalun, 金城　順英, 田中　宏幸, 森元　聡

日本薬学会第130年会  2010.3 

Development of fluorescence-linked immunosorbent assay using a fusion protein of green fluorescent protein and single chain variable fragment antibody against bioactive naphthoquinone, plumbagin International conference

Sakamoto S., Taura F., Putalun W., Pongkitwitoon B., Tsuchihashi R., Kinjo J., Tanaka H., Morimoto S.

Asian Symposium for Pharmaceutical Science in JSPS Asian Core Program  2009.11 

Expression, purification, and characterization of anti plumbagin single-chain variable fragment antibody in Sf9 insect cells International conference

Sakamoto S., Taura F., Tsuchihashi R., Putalun W., Kinjo J., Tanaka H., Morimoto S.

Asian Federation for Pharmaceutical Sciences 2009  2009.10 

Determination of vitamin K3 by an indirect competitive ELISA using monoclonal antibodies against plumbagin International coauthorship International conference

Sakamoto S., Sakoda J., Morinaga O., Putalun W., Tsuchihashi R., Morimoto S., Kinjo J., Tanaka H.

NRCT JSPS Core University Program on Natural Medicine in Pharmaceutical Sciences, The 8th Joint Seminar (INNOVATIVE RESEARCH IN NATURAL PRODUCTS FOR SUSTAINABLE DEVELOPMENT)  2009.2 

Construction and expression of specificity-improved single-chain variable fragments against the bioactive naphthoquinone, plumbagin and its application in an enzyme-linked immunosorbent assay International conference

Sakamoto S., Taura F., Putalun W., Tsuchihashi R., Morimoto S., Kinjo J., Tanaka H.

The 4th KSP-JSP-CCTCNM Joint Symposium on Pharmacognosy (KOREA-JAPAN-CHINA Joint Symposium 2008)  2008.6 

抗plumbagin小型化抗体(抗PL-scFv)の作製及びELISAへの応用研究

坂元　政一, 田浦　太志, Waraporn Putalun, 土橋　良太, 森元　聡, 田中　宏幸, 金城　順英

日本薬学会第128年会  2008.3 

抗plumbagin抗体の作製及びELISAへの応用

坂元　政一, Waraporn Putalun, 土橋　良太, 金城　順英, 田中　宏幸

日本生薬学会第54年会  2007.9 

Synthesis of strictosidine using recombinant strictosidine synthase, and its stability International conference

Sakamoto S., Goto R., Thi H.H.A., Nuntawong P., Morimoto S.

PST Conference 2024  2024.5 

Rapid dual detection of glycyrrhizin and sennoside a in Daiokanzoto preparations via lateral flow immunoassay International coauthorship International conference

Nuntawong P., Ochi A., Chaingam J., Ota T., Juengwatanatrakul T., Putalun W., Shoyama Y., Morimoto S., Sakamoto S.

PST Conference 2024  2024.5 

Simultaneous detection system for hesperidin, glycyrrhizin and ginsenoside Rb1 by quantum dot-based immunoassay for quality assessment of Rikkunshito International conference

Noguchi K., Kido Y., Nuntawong P., Morimoto S., Sakamoto S.

PST Conference 2024  2024.5 

サイコサポニンb2に対するモノクローナル抗体の作製および間接競合ELISAへの応用

藤井　俊輔、越智　昭裕、太田　智絵、宇都　拓洋、田中　宏幸、森元　聡、坂元　政一

日本生薬学会第69回年会  2023.9 

Immunoassay for the quality control of Citrus unshiu peel International conference

Noguchi K., Imahori D., Kido Y., Nuntawong P., Tanaka H., Morimoto S., Sakamoto S.

PST Conference 2023  2023.6 

Detection of kwakhurin via bioimprinted protein International conference

Sakamoto S., Minami K., Nuntawong P., Yusakul G., Putalun W., Tanaka H., Fujii S., Morimoto S

PST Conference 2023  2023.6 

Establishment of a quality evaluation method for Daio-kanzo-to using fluorescent-labeled antibody International conference

Ochi A., Tanaka H., Morimoto S., Sakamoto S.

PST Conference 2023  2023.6 

Development of aptamer-based detection method to quantify berberine in Kampo medicine samples

Poomraphie Nuntawong、田山　順恵、森元　聡、坂元　政一

日本薬学会第143年会  2023.3 

大黄甘草湯の迅速な品質評価法の確立を目指した二成分同時分析系の構築

越智　昭裕、田中　宏幸、正山　征洋、森元　聡、坂元　政一

日本薬学会第143年会  2023.3 

Selection, characterization, and application of the aptamer for berberine detection method

Poomraphie Nuntawong、坂元　政一、森元　聡

日本生薬学会第68回年会  2022.9 

ヘスペリジンに対するモノクローナル抗体の作製及びLateral Flow Immunoassay (LFA)への応用

野口　幹太、新川　裕生、Poomraphie Nuntawong、坂元　政一、森元　聡

日本生薬学会第68回年会  2022.9 

甘草の育種研究に向けた抗グリチルリチン小型化抗体(GC-scFv)遺伝子の特徴付け

淵上　里緒、越智　昭裕、田中　宏幸、宇都　拓洋、正山　征洋、坂元　政一、森元　聡

日本生薬学会第68回年会  2022.9 

Chaparrinon: Eurycoma harmandiana Pierre identical marker and the studies of anti-inflammatory effect International coauthorship

Jiranan Chaingam、坂元　政一、Thaweesak Juengwatanatrakul、Gorawit Yusakul、Tripetch Kanchanapoom、Waraporn Putalun

日本生薬学会第68回年会  2022.9 

Determination of swertiamarin and related compounds in the gastrointestinal diseases folk medicine, Swertia japonica Makino using immunoassays

Poomraphie Nuntawong, Taiki Horikawa, Hiroyuki Tanaka, Seiichi Sakamoto, Satoshi Morimoto

2022.3 

抗ヘスペリジンモノクローナル抗体の作製及び間接競合ELISAの確立

野口　幹太、藤井　俊輔、田中　宏幸、坂元　政一、森元　聡

日本薬学会第142年会  2022.3 

Synthesis of harringtonine ester derivatives possessing potent anti-leukemia activities International conference

Ochi A., Miyamoto T., Yoritate M., Tanaka H., Hirai G., Sakamoto S., Morimoto S

4th International Conference on Traditional Medicine, Phytochemistry and Medicinal Plants (TMedPM-2020)  2022.2 

Quantitative analysis of glycyrrhizin using rapid magnetic particles-based enzyme immunoassay for the quality control of Glycyrrhiza spp. International conference

Sakamoto S., Minami K., Yusakul G., Putalun W., Tanaka H., Morimoto S

4th International Conference on Traditional Medicine, Phytochemistry and Medicinal Plants (TMedPM-2020)  2022.2 

Prussian blue nanoparticles based lateral flow assay for detection of the sport-prohibited-compound, higenamine International conference

Nuntawong P., Tanaka H., Sakamoto S., Morimoto S

4th International Conference on Traditional Medicine, Phytochemistry and Medicinal Plants (TMedPM-2020)  2022.2 

Production of anti-hesperidin monoclonal antibody and establishment of quantitative analysis method by ELISA International conference

Noguchi K., Fujii S., Tanaka H., Sakamoto S., Morimoto S.

4th International Conference on Traditional Medicine, Phytochemistry and Medicinal Plants (TMedPM-2020)  2022.2 

Development of icELISA for the detection of the prohibited doping agent higenamine International conference

Nuntawong P., Tanaka H., Sakamoto S., Morimoto S

The 11th JSP/CSP/KSP Joint Symposium on Pharmacognosy  2021.9 

磁気ビーズイムノアッセイを用いたプエラリア・ミリフィカに含有するクワクリンの定量分析法の確立

坂元　政一、衛藤　亮太、Poomraphie Nuntawong、Gorawit Yusakul、Waraporn Putalun、藤井　俊輔、田中　宏幸、森元　聡

日本薬学会第141年会  2021.3 

Synthesis and evaluation of anti-leukemia activity of harringtonine ester derivatives International conference

Ochi A., Miyamoto T., Yoritate M., Tanaka H., Sakamoto S., Morimoto S

3rd International Conference on Traditional Medicine, Phytochemistry and Medicinal Plants (TMedPM-2020)  2020.11 

Development of magnetic particles-based enzyme immunoassay for the detection of kwakhurin in Pueraria candollei International conference

Sakamoto S., Eto R., Nuntawong P., Yusakul G., Juengwatanatrakul T., Putalun W., Fujii S., Tanaka H., Morimoto S.

3rd International Conference on Traditional Medicine, Phytochemistry and Medicinal Plants (TMedPM-2020)  2020.11 

Bioimprinting technique for the quantitative analysis of kwakhurin International conference

Minami K., Yusakul G., Fujii S., Sakamoto S., Morimoto S

3rd International Conference on Traditional Medicine, Phytochemistry and Medicinal Plants (TMedPM-2020)  2020.11 

ピロリチジンアルカロイド、モノクロタリンの高感度免疫化学的測定法の確立

坂元　政一、Gorawit Yusakul、長光　梨花、田中　宏幸、森元　聡

日本薬学会第140年会  2020.3 

三分割GFP を用いたハリントニンの新規免疫学的蛍光測定法の開発

森保　歩惟、小松　頌平、坂元　政一、田中　宏幸、森元　聡

日本生薬学会第66回年会  2019.9 

プエラリア・ミリフィカ特有成分“Kwakhurin”の高感度イムノアッセイの確立

南　圭、磨田　瑠衣、Gorawit Yusakul、田中　宏幸、坂元　政一、森元　聡

日本生薬学会第66回年会  2019.9 

ダイズイソフラボン配糖体の検出を目的としたオープンサンドイッチ蛍光イムノアッセイの開発

内山　裕貴、坂元　政一、田中　宏幸、森元　聡

日本生薬学会第66回年会  2019.9 

重鎖定常領域置換による抗フォルスコリンFab の巻き戻し効率の向上と定量分析への応用

坂元　政一、Gorawit Yusakul、田中　宏幸、森元　聡

日本生薬学会第66回年会  2019.9 

主要総大豆イソフラボン配糖体の免疫化学的分析法の確立

坂元　政一、Gorawit Yusakul、Benyakan Pongkitwitoon、田中　宏幸、森元　聡

日本薬学会第139年会  2019.3 

セイロンマツリ由来ポリケタイド合成酵素(polyketide synthase: PKS)に関する研究

森田　優衣、坂元　政一、田中　宏幸、森元　聡

第35回日本薬学会九州支部大会  2018.11 

コカ由来III型ポリケタイド合成酵素についての研究

松浦　圭祐、坂元　政一、田中　宏幸、森元　聡

日本生薬学会第65回年会  2018.9 

磁気ビーズを用いた苦味配糖体「アマロゲンチン」の免疫化学的分析法の開発

衛藤 亮太、和田　伸司、山口　朋子、坂元　政一、田中　宏幸、森元　聡

日本生薬学会第65回年会  2018.9 

コガネバナ由来ペルオキシダーゼに対する中和抗体の作製及びその応用

杉山　菜摘、坂元　政一、田中　宏幸、森元　聡

日本生薬学会第65回年会  2018.9 

Development of immunoassay for analysis of homoharringtonine in plant samples International conference

Pongkitwitoon P., Sakamoto S., Nagamitsu R., Putalun W., Tanaka H., Morimoto S.

22nd International Phytopharm Congress 2018  2018.6 

Expression and characterization of antigen binding fragment (Fab) against isomiroestrol in E.coli Shuffle® International coauthorship International conference

Juengsanguanpornsuk W., Kitisripanya T., Yusakul G., Srisongkram T., Krittanai S., Sakamoto S., Tanaka H., Putalun W.

The 5th International Conference on Current Drug Development 2018 (CDD 2018) and The 3rd International Conference on Herbal and Traditional Medicine 2018 (HTM 2018)  2018.5 

Antiproliferative activity of sodium periodate-mediated harringtonine derivatives against HL-60 acute leukemia cells International conference

Sakamoto S., Miyamoto T., Usui K., Tanaka H., Morimoto S.

The 5th International Conference on Current Drug Development 2018 (CDD 2018) and The 3rd International Conference on Herbal and Traditional Medicine 2018 (HTM 2018)  2018.5 

Construction and expression of single-chain fragment against miroestrol in silkworm International conference

Chaingam J., Kitisripanya T., Sakamoto S., Tanaka H., Morimoto S., Putalun W.

The 5th International Conference on Current Drug Development 2018 (CDD 2018) and The 3rd International Conference on Herbal and Traditional Medicine 2018 (HTM 2018)  2018.5 

Expression and characterization of anti-miroestrol fragment antigen binding in E. coli International conference

Krittanai S., Yusakul G., Sakamoto S., Tanaka H., Putalun W.

The 5th International Conference on Current Drug Development 2018 (CDD 2018) and The 3rd International Conference on Herbal and Traditional Medicine 2018 (HTM 2018)  2018.5 

苦味配糖体アマロゲンチンに対する迅速分析法の開発

和田 伸司, 坂元 政一, 田中 宏幸, 森元 聡

日本生薬学会第64回年会  2017.9 

The expression of anti-ganoderic acid A single chain variable fragment antibody using Escherichia coli expression systems and their reactivity International coauthorship International conference

Sakamoto S., Nuntawong P., Yusakul G., Ratnatilaka Na Bhuket P., Kohno T., Kikkawa N., Rojsitthisak P., Shimizu K, Tanaka H., Morimoto S.

International Annual Meeting in Pharmaceutical Sciences (JSPS-NRCT 2017 and IAMPS 33)  2017.3 

セファロタキサス属アルカロイド、ハリントニンの高感度免疫定量法の確立

坂元 政一, Gorawit Yusakul, 長光 梨花, 常浦 裕未, Warapon Putalun, 宮本 智文, 田中 宏幸, 森元 聡

日本生薬学会第63回年会  2016.9 

抗ganoderic acid Aモノクローナル抗体を用いた免疫クロマトグラフィーの確立

橘川 奈生, 坂元 政一, 河野 俊享, 清水 邦義, 田中 宏幸, 森元 聡

日本生薬学会第63回年会  2016.9 

Production and characterization of monoclonal antibody against the isoflavone glycoside daidzin International conference

Yusakul G., Sakamoto S., Juengwatanatrakul T., Putalun W., Tanaka H., Morimoto S.

The 4th Current Drug Development International Conference (CDD 2016)  2016.6 

Production of monoclonal antibodies against pyrrolizidine alkaloid, retronecine and its potential applications International conference

Nagamitsu R., Sakamoto S., Tanaka H., Morimoto S.

The 6th International Conference on Natural Products for Health and Beauty (NATPRO 6)  2016.1 

Cloning, expression, and purification of polyketide synthase of Erythrooxylum coca International conference

Yamamoto C., Sakamoto S., Tanaka H., Morimoto S.

The 6th International Conference on Natural Products for Health and Beauty (NATPRO 6)  2016.1 

抗グリチルリチンモノクローナル抗体を機軸とした応用研究～機能解析からミサイルタイプの分子育種～

宇都 拓洋, 坂元 政一, 迫田 淳一, 榮田 まり子, 森永 紀, 田中 宏幸, 正山 征洋

第7回甘草に関するシンポジウム  2015.7 

ELISA による霊芝トリテルペノイドganoderic acid A の迅速かつ高感度定量法の確立

河野　俊享, 清水 邦義, 坂元　政一, 田中 宏幸, 森元 聡

日本薬学会第135年会  2015.3 

抗D-グルタミン酸モノクローナル抗体の作製とその特徴付け

常浦　祐未, 松浦　ゆりの, 米永　弥生, 坂元　政一, 麻生 真理子, 黒瀬 等, 田中 宏幸, 森元 聡

日本薬学会第135年会  2015.3 

Development of an indirect competitive enzyme-linked immunosorbent assay (icELISA) using highly specific monoclonal antibody against ganoderic acid A International conference

Kohno T., Sakamoto S., Tanaka H., Morimoto S.

The 1st International Conference on Herbal and Traditional Medicine (HTM 2015)  2015.1 

Construction and characterization of the antigen-binding antibody fragment against paclitaxel International conference

Yusakul G., Sakamoto S., Tanaka H., Morimoto S.

The 1st International Conference on Herbal and Traditional Medicine (HTM 2015)  2015.1 

植物アポトーシスにおけるシロイヌナズナ由来Cytochromecの機能解析

中島　由貴, 田中 宏幸, 坂元　政一, 森元　聡

日本生薬学会第61回年会  2014.9 

ケシ由来パーオキシダーゼの機能解析

堀　太樹, 坂元　政一, 田中 宏幸, 森元　聡

日本生薬学会第61回年会  2014.9 

植物ネクローシスにおけるcyclophilin Dの機能解析と抗体作製

加藤　梨那, 中村　俊介, 相星　彩佳, 坂元　政一, 田畑　香織, 田中 宏幸, 森元　聡

日本生薬学会第61回年会  2014.9 

Enhanced plumbagin production in hairy root culture of Plumbago zeylanica by anti-plumbagin single-chain fragment antibody International conference

Sakamoto S., Putalun W., Pongkitwitoon B., Shoyama Y., Tanaka H., Morimoto S.

The 8th JSP-CCTCNM-KSP Joint Symposium on Pharmacognosy  2014.9 

Development of an immunoassay using anti-wogonin glucuronide monoclonal antibody International conference

Paudel M.K., Sakamoto S., Tanaka H., Morimoto S.

The 8th JSP-CCTCNM-KSP Joint Symposium on Pharmacognosy  2014.9 

Tubulin-polymerizing activity of Ganoderma triterpenoids and production of their monoclonal antibody International conference

Kohno T., Bang. H.T., Shimizu K., Sakamoto S., Tanaka H., Morimoto S.

The 8th JSP-CCTCNM-KSP Joint Symposium on Pharmacognosy  2014.9 

植物ネクローシスにおけるcyclophilin Dの機能解析

加藤　梨那, 中村　俊介, 相星　彩佳, 坂元　政一, 田畑　香織, 田中 宏幸, 森元　聡

日本薬学会第134年会  2013.3 

新規D-アミノ酸分析法開発を目指した抗D-セリンモノクローナル抗体の作製

常浦　祐未, 田畑　香織, 坂元　政一, 田中 宏幸, 麻生 真理子, 末宗 洋, 森元　聡

日本薬学会第134年会  2013.3 

Langmuir単分子膜手法による脂質ラフトモデルにおけるグリチルレチン酸の界面科学的挙動解析

坂元　政一, 中原　広道, 柴田　攻

第64回コロイドおよび界面化学討論会  2013.9 

新規長鎖部分フッ素化アルコール(F6H9OH, F6H11OH)と生体膜モデルDPPCとの単分子膜混和挙動

中原　広道, 山田　高義, 坂元　政一, 柴田　攻

第64回コロイドおよび界面化学討論会  2013.9 

Solubilization capacity of a gemini surfactant of bis(tetradecyldimethylammonium)decane International conference

Nakahara H., Sakamoto S., Kojima Y., Hasegawa A., Akisada H., Shibata O.

27th Conference of the European Colloid and Interface Society (27th ECIS)  2013.9 

Interaction of glycyrrhetinic acid with lipid rafts model by a Langmuir monolayer International conference

Sakamoto S., Nakahara H., Shibata O.

13th EUROPEAN CONFERENCE on ORGANISED FILMS (ECOF13)  2013.7 

Perfluoroalkyl基を有した長鎖アルコールのLangmuir単分子膜挙動

上原　聖奈, 中原　広道, 藤田　一郎, 坂元　政一, 中村　承平, 柴田　攻

日本薬学会第133年会  2013.3 

ジェミニ型界面活性剤(14-12-14,2Br-)の溶液物性：難溶性物質の可溶化について

小島　由意, 長谷川　温子, 中原　広道, 坂元　政一, 秋貞　英雄, 柴田　攻

日本薬学会第133年会  2013.3 

脂質ラフト生体膜モデルにおけるグリチルレチン酸の単分子膜挙動解析

坂元　政一, 中原　広道, 柴田　攻

第29回日本薬学会九州支部大会  2012.12 

新規部分フッ素化アルコール(FnHmOH)に関する各種物性の熱力学的解析

上原　聖奈, 中原　広道, 藤田　一郎, 坂元　政一, 中村　承平, 柴田　攻

第29回日本薬学会九州支部大会  2012.12 

新規部分フッ素化両親媒性物質(FnHmOH)とDPPCの二成分単分子膜挙動

臼井　千尋, 平野　千佳代, 中原　広道, 坂元　政一, 柴田　攻

第29回日本薬学会九州支部大会  2012.12 

ジェミニ型界面活性剤ミセルへのn-アルキルベンゼンの可溶化挙動

小島　由意, 長谷川　温子, 中原　広道, 坂元　政一, 柴田　攻

第29回日本薬学会九州支部大会  2012.12 

部分フッ素化両親媒性物質(FnHmOH)とDPPCの二成分混和性の解析

臼井　千尋, 平野　千佳代, 中原　広道, 坂元　政一, 柴田　攻

平成24年度物理化学インターカレッジセミナー兼油化学界面科学部会九州地区講演会  2012.11 

脂質ラフトモデルにおけるグリチルレチン酸の界面科学的挙動の解析～Langmuir単分子膜～

坂元　政一, 中原　広道, 柴田　攻

平成24年度物理化学インターカレッジセミナー兼油化学界面科学部会九州地区講演会  2012.11 

新規部分フッ素化アルコール(FnHmOH)の単分子膜挙動

上原　聖奈, 中原　広道, 藤田　一郎, 坂元　政一, 中村　承平, 柴田　攻

平成24年度物理化学インターカレッジセミナー兼油化学界面科学部会九州地区講演会  2012.11 

Interaction of glycyrrhizin with lipid rafts model at the air/water interface International conference

Sakamoto S., Nakahara H., Shibata O.

2012.9 

Surface property of a partially fluorinated amphiphile (F8HmOH): Binary miscibility with dipalmitoylphosphatidylcholine International conference

Hirano C., Nakahara H., Sakamoto S., Krafft M.P., Shibata O.

2012.9 

Efficient silkworm expression of a fluorescent single domain antibody against plumbagin using Bombyx mori nucleopolyhedrovirus bacmid DNA system International conference

Sakamoto S., Pongkitwitoon B., Puatlun W., Maenaka K., Tanaka H., Shibata O., Morimoto S.

2012.9 

Construction and expression of fluobody against ginsenosides for the fluorescence-based immunoassay International conference

Sakamoto S., Tanizaki Y., Pongkitwitoon B., Shibata O., Tanaka H., Morimoto S.

2012.9 

Construction and expression of fluobody against amphiphilic saponin, ginsenosides for fluorescence-linked immunosorbent assay International conference

Sakamoto S., Tanizaki Y., Pongkitwitoon B., Shibata O., Tanaka H., Morimoto S.

26th Conference of the European Colloid and Interface Society (26th ECIS)  2012.9 

Langmuir monolayerbehavior of partially fluorinated alcohols (F8HmOH) and dipalmitoylphosphatidylcholine (DPPC) International conference

Nakahara H., Hirano C., Sakamoto S., Krafft M.P., Shibata O.

26th Conference of the European Colloid and Interface Society (26th ECIS)  2012.9 

Langmuir monolayer properties of a series of partially fluorinated alcohols International conference

Sakamoto S., Nakahara H., Fujita I., Nakamura S., Nakaya M., Kanie K., Shibata O.

26th Conference of the European Colloid and Interface Society (26th ECIS)  2012.9 

Synthesis, characterization, and surface property of partially fluorinated long-chain alcohols (FnHmOH) International conference

Nakahara H., Sakamoto S., Fujita I., Nakamura S., Nakaya M., Kanie K., Matsumura A., Shibata O.

2012.7 

A chimera of green fluorescent protein with single chain variable fragment antibody against amphiphilic saponin, ginsenosides for novel immunoassay International conference

Sakamoto S., Tanizaki Y., Pongkitwitoon B., Shibata O., Tanaka H., Morimoto S.

2012.7 

Interfacial behavior of glycyrrhizin interacted with lipid rafts model by Langmuir monolayer study International conference

Sakamoto S., Nakahara H., Shibata O.

2012.7 

Binary miscibility of partially fluorinated long-chain alcohols (F8HnOH) with dipalmitoylphosphatidylcholine (DPPC) at the air/water interface International conference

Nakahara H., Hirano C., Sakamoto S., Krafft M.P., Shibata O.

2012.7 

Molecular cloning and expression of O-methyltransferases from opium poppy International conference

Pongkitwitoon B., Sakamoto S., Sasaki K., Tanaka H., Morimoto S.

2012.7 

Development of an enzyme-linked immunosorbent assay for the determination of natural Amphiphilic compounds, ginsenosides in ginseng International conference

Sakamoto S., Pongkitwitoon B., Tanaka H., Shibata O., Morimoto S.

14th International Association of Colloid and Interface Scientists (IACIS) Conference  2012.5 

Interfacial behavior of glycyrrhizin interacted with lipid rafts model by Langmuir monolayer study International conference

Sakamoto S., Shibata O., Nakahara H.

14th International Association of Colloid and Interface Scientists (IACIS) Conference  2012.5 

Expression of a fluorescent single domain antibody against plumbagin in silkworm larvae for the development of fluorescence-linked immunosorbent assay International conference

Sakamoto S., Pongkitwitoon B., Putalun W., Maenaka K., Tanaka H., Shibata O., Morimoto S.

14th International Association of Colloid and Interface Scientists (IACIS) Conference  2012.5 

コガネバナβ-グルクロニダーゼの反応メカニズムに関する研究

今村　聡, 今村　麻美, 坂元　政一, 田畑　香織, 田中　宏幸, 森元　聡

日本薬学会第132年会  2012.3 

組換え抗フォルスコリンFabの構築とその機能評価

田中　宏幸, 坂元　政一, 首藤　美奈, 椙山　えみ香, 森元　聡, 正山 征洋

日本薬学会第132年会  2012.3 

ケシ由来O-methyltransferaseの遺伝子クローニングと発現

Benyakan Pongkitwitoon, 坂元　政一, 田畑　香織, 田中　宏幸, 森元　聡

日本薬学会第132年会  2012.3 

新規部分フッ素化アルコール(F4H11OH)の単分子膜挙動とDPPCとの混和性の検討

平野　千佳代, 大嶺　彩, 甲斐　翔子, 中原　広道, 坂元　政一, 柴田　攻

第28回日本薬学会九州支部大会  2011.12 

脂質ラフトモデルにおけるグリチルリチンの界面科学的挙動

坂元　政一, 中原　広道, 柴田　攻

第28回日本薬学会九州支部大会  2011.12 

新規部分フッ素化両親媒性物質の合成とそのフッ素化度の検討

藤田　一郎, 岡橋　慶紀, 川畑　紀健, 北口　大介, 中原　広道, 坂元　政一, 柴田　攻

第28回日本薬学会九州支部大会  2011.12 

フッ素化度の異なる長鎖アルコールの合成とその基本物性

藤田　一郎, 岡橋　慶紀, 川畑　紀健, 北口　大介, 中原　広道, 坂元　政一, 柴田　攻

平成23年度物理化学インターカレッジセミナー兼油化学界面科学部会九州地区講演会  2011.11 

カイコ-バキュロウィルス発現系を用いた抗プルンバギン蛍光抗体の発現及び高感度蛍光イムノアッセイへの応用

坂元　政一, Benyakan Pongkitwitoon, 田中　宏幸, 森元　聡, 前仲　勝実, 柴田　攻

平成23年度物理化学インターカレッジセミナー兼油化学界面科学部会九州地区講演会  2011.11 

抗ジンセノシドRe小型化抗体を用いたニンジンの品質評価法の確立

坂元　政一, Benyakan Pongkitwitoon, 田中　宏幸, 森元　聡, 大嶺　彩, 柴田　攻

平成23年度物理化学インターカレッジセミナー兼油化学界面科学部会九州地区講演会  2011.11 

(Perfluorobutyl)pentadecanol (F4H11OH)とDPPCとの2成分Langmuir単分子膜挙動

平野　千佳代, 大嶺　彩, 甲斐　翔子, 中原　広道, 坂元　政一, 柴田　攻

平成23年度物理化学インターカレッジセミナー兼油化学界面科学部会九州地区講演会  2011.11 

A fluorescent single domain antibody against plumbagin expressed in　silkworm larvae for fluorescence-linked immunosorbent assay (Next-generation immunoassay using antibody) International coauthorship International conference

Sakamoto S., Pongkitwitoon B., Sasaki-Tabata K., Putalun W., Maenaka K., Shibata O., Tanaka H., Morimoto S.

The 25th European Colloid and Interface Society  2011.9 

GFP融合小型化抗体を用いた新規免疫化学的分析法の開発

谷崎　裕介, 坂元　政一, 田中　宏幸, 森元　聡

第27回日本薬学会九州支部大  2010.12 

Fluobody against plumbagin and its application to novel fluorescence-linked immunosorbent assay International conference

Sakamoto S., Taura F., Pongkitwitoon B., Putalun W., Tsuchihashi R., Kinjo J., Tanaka H., Morimoto S.

The 57th Annual Meeting of the Japanese Society of Pharmacognosy/The 5th JSP-CCTNM-KSP Joint Symposium on Pharmacognosy in Tokushima, 2010  2010.9 

Enhanced plumbagin production from in vitro cultures of Drosera indica L. by elicitation International conference

Juengwatanatrakul T., Sakamoto S., Tanaka H., Putalun W.

The 1st Current Drug Development International Conference  2010.5 

Enhanced plumbagin production from in vitro cultures of Drosera burmanii using elicitation International conference

Putalun W., Udomsin O., Yusakul G., Juengwatanatrakul T., Sakamoto S., Tanaka H.

The 1st Current Drug Development International Conference  2010.5 

Determination of puerarin and its aglycone daidzein by an enzyme-linked immunosorbent assay using anti-puerarin polyclonal antibody International conference

Pongkitwitoon B., Sakamoto S., Tanaka H., Tsuchihashi R., Kinjo J., Morimoto S., Putalun W.

The 1st Current Drug Development International Conference  2010.5 

Determination of total isoflavonoids in Pueraria Candollei by enxyme-linked immunosorbent assay using anti-puerarin and anti-daidzin polyclonal antibodies International conference

Pongkitwitoon B., Sakamoto S., Tanaka H., Tsuchihashi R., Kinjo J., Morimoto S., Putalun W.

Asian Federation for Pharmaceutical Sciences 2009  2009.10 

Construction and expression of anti-glycyrrhizin singlechain variable fragment and its application International conference

Sakoda J., Sakaeda M., Sakamoto S., Morinaga O., Uto T., Shoyama Y., Tanaka H.

NRCT JSPS Core University Program on Natural Medicine in Pharmaceutical Sciences, The 8th Joint Seminar (INNOVATIVE RESEARCH IN NATURAL PRODUCTS FOR SUSTAINABLE DEVELOPMENT)  2009.2 

Production and characterization of polyclonal antibody against daidzin International conference

Pongkitwitoon B., Sakamoto S., Tsuchihashi R., Kinjo J., Tanaka H., Putalun W.

NRCT JSPS Core University Program on Natural Medicine in Pharmaceutical Sciences, The 8th Joint Seminar (INNOVATIVE RESEARCH IN NATURAL PRODUCTS FOR SUSTAINABLE DEVELOPMENT)  2009.2 

Construction and expression of specificity-improved single-chain variable fragments (scFv) against bioactive naphthoquinone, plumbagin International coauthorship International conference

Sakamoto S., Taura F., Putalun W., Tsuchihashi R., Morimoto S., Kinjo J., Tanaka H.

NRCT JSPS Core University Program on Natural Medicine in Pharmaceutical Sciences, The 8th Joint Seminar (INNOVATIVE RESEARCH IN NATURAL PRODUCTS FOR SUSTAINABLE DEVELOPMENT)  2009.2 

Construction and expression of specificity-improved single-chain variable fragments against bioactive naphthoquinone, plumbagin International coauthorship International conference

Sakamoto S., Taura F., Putalun W., Tsuchihashi R., Morimoto S., Kinjo J., Shoyama Y., Tanaka H.

Shanghai International Symposium on pharmacy  2008.12 

抗グリチルリチン小型化抗体の作製とカンゾウ育種への応用

迫田　淳一, 榮田　まり子, 坂元　政一, 田中　宏幸, 森永　紀, 宇都　拓洋, 正山　征洋

日本生薬学会第55年会  2008.9 

Development of an enzyme-linked immunosorbent assay (ELISA) using highly-specific monoclonal antibodies against plumbagin International conference

Sakamoto S., Putalun W., Tsuchihashi R., Morimoto S., Kinjo J., Tanaka H.

Asian Symposium for Pharmaceutical Science in JSPS Asian Core Program  2007.10 

ヒト腸内細菌株による日本産葛花イソフラボンの代謝について

土橋　良太, 小寺　満, 坂元　政一, 金城　順英, 中島　嘉次郎, 細野　剛, 山崎　律, 新甫　勇次郎, 野原　稔弘

日本生薬学会第53年会  2006.9 

ヒト腸内細菌による葛花イソフラボンの代謝について

土橋　良太, 坂元　政一, 金城　順英, 中島　嘉次郎, 細野　剛, 山崎　律, 新甫　勇次郎, 野原　稔弘

日本生薬学会第52年会  2005.9 

FLASH "大麻の生理活性物質を効率よくつくる" Nature 567, 123, (2019) 解説

坂元　政一

現代化学   2019.5

第8回　タイ王国の薬学現場から

坂元　政一

公益財団法人日本薬学会　ファルマシア   2018.6

Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites

Sakamoto S., Putalun W., Vimolmangkang S., Phoolcharoen W., Shoyama Y., Tanaka H., Morimoto S.

Journal of Natural Medicines   2018.1

有用アルカロイドを産生する植物細胞の効率的な解析方法

坂元　政一

公益財団法人日本薬学会　ファルマシア   2017.5

Towards the prophylactic and therapeutic use of human neutralizing monoclonal antibodies for Middle East respiratory syndrome coronavirus (MERS-CoV)

Sakamoto S., Tanaka H., Morimoto S.

Annals of Translational Medicine   2015.3

Application of green fluorescent protein in immunoassays

Sakamoto S., Shoyama Y., Tanaka H., Morimoto S.

Advances in Bioscience and Biotechnology   2014.5

Fluobodies against bioactive natural products and their application in fluorescence-linked immunosorbent assay

Sakamoto S., Pongkitwitoon B., Nakahara H., Shibata O., Shoyama Y., Tanaka H., Morimoto S.

Antibodies   2012.9

バイオインプリンティング技術を用いたグリチルリチンのハイスループット分析法の開発　～日本薬局方規格基準の甘草の育種研究に向けて～

坂元　政一

公益財団法人日本食品化学研究振興財団　第26回研究成果報告書   2020.12

オープンサンドイッチ蛍光イムノアッセイの確立および 大豆イソフラボンのハイスループット分析

坂元　政一

公益財団法人不二たん白質研究振興財団　平成27年度 助成研究報告書   2016.9

免疫クロマトグラフィーを用いた大豆イソフラボンのワン・ステップ解析

坂元　政一

公益財団法人タカノ農芸化学研究助成財団　平成26年度助成研究報告書   2015.9

生体膜モデルにおける天然由来食品添加物グリチルリチンの分子挙動解析

坂元　政一

公益財団法人日本食品化学研究振興財団　第20回研究成果報告書   2014.12

生体膜モデルにおける天然由来食品添加物グリチルリチンの分子挙動解析

坂元　政一

公益財団法人日本食品化学研究振興財団　第19回研究成果報告書   2013.12

Molecular breeding of solasodine glycoside for Solanum khasianum using anti-solamargine single-chain Fv gene

Putalun W., Tanaka H., Sakamoto S., Shoyama Y.

Medicinal & Aromatic Plants   2013.4

Visual detection and quantitative analysis of bioactive compound contained in traditional Chinese medicines based on new eastern blotting

Morinaga O., Uto T., Sakamoto S., Tanaka H., Shoyama Y.

Trends in Chromatography   2007.3

Session Chair（Session-3 Physical and Analytical Chemistry）

（ 九州大学 ） 2021.8

Screening of academic papers

Role(s)： Peer review

2021

Screening of academic papers

Role(s)： Peer review

2020

Screening of academic papers

Role(s)： Peer review

2019

Current Pharmaceutical Biotechnology International contribution

2018.7 - Present

Screening of academic papers

Role(s)： Peer review

2018

ワークショップ実行委員 International contribution

2017.8

Screening of academic papers

Role(s)： Peer review

2017

真菌の生成物に関する研究

2022

免疫化学的測定法を基盤とした生薬及び漢方製剤の品質評価法の開発及び標準化に関する研究

2021 - 2023

国立研究開発法人日本医療研究開発機構 (AMED)、医薬品等規制調和・評価研究事業

神経変性疾患治療への応用を目指した新規脳内糖脂質代謝の解析

2021

令和3年度、理研－九大科学技術ハブ共同研究プログラム

ヒュウガトウキ中のYN-1に関する研究

2021

Pictet-Spengler反応を触媒する抗体の作製と薬用植物育種への展開

Grant number：20K07101  2020 - 2022

日本学術振興会  科学研究費助成事業  基盤研究(C)

ハリントニンエステル誘導体の合成及び各種腫瘍細胞に対する増殖阻害活性試験

2020

令和2年度、公益財団法人、ふくおか公衆衛生推進機構、がん研究助成金(奨励賞)

ヒュウガトウキに関する研究

2020

大豆イソフラボン配糖体のオープンサンドイッチ蛍光イムノアッセイ

2020

令和2年度、九州大学研究活動基礎支援制度、外国語校閲経費支援

プエラリア・ミリフィカ含有健康食品の安全性評価法の確立

Grant number：19KA0101  2019 - 2020

科学研究費助成事業  厚生労働科学研究費補助金 (厚生労働省)

バイオインプリンティング技術を用いたグリチルリチンのハイスループット分析法の開発　～日本薬局方規格基準の甘草の育種研究に向けて～

2019

平成31年度、第26回公益財団法人、日本食品化学研究振興財団、研究助成金

甘草の育種研究

2019

共同研究、日本電気株式会社、新規蛍光タンパク質の遺伝子情報を用いた発光植物の研究

2017.7 - 2018.7

共同研究

三分割GFPを基盤とした免疫化学的蛍光センサーの開発

Grant number：17K15466  2017 - 2019

科学研究費助成事業  若手研究(B)

抗体工学を応用した有用薬用植物の作出研究

Grant number：16K15131  2016 - 2017

科学研究費助成事業  挑戦的萌芽研究

三分割GFPを基盤とした迅速蛍光センサーの開発 ～抗白血病薬ハリントニンをモデルに～

2016

平成28年度、QRプログラム、わかばチャレンジ

Development of highly sensitive immunological techniques for determination of cephalotaxus alkaloids, harringtonine

2016

Pictet-Spengler反応触媒抗体の作製及び効率的なスクリーニング法の開発

2015 - 2016

平成27年度、公益財団法人、武田科学振興財団、薬学系研究奨励

オープンサンドイッチ蛍光イムノアッセイの確立及び大豆イソフラボンのハイスループット分析

2015

平成27年度、公益財団法人、不二たん白質研究振興財団、平成27年度(第19期)若手研究者枠研究助成金

Fluorobody を用いた植物細胞死の網羅的解析

Grant number：26670046  2014 - 2015

科学研究費助成事業  挑戦的萌芽研究

D-アミノ酸の薬理学的機能解析を目指したイムノアッセイの開発

2014

平成26年度、日本科学協会、笹川科学研究助成金

免疫クロマトグラフィーを用いた大豆イソフラボンのワン・ステップ解析

2014

平成26年度、公益財団法人、タカノ農芸化学研究助成財団、研究助成金(若手研究者部門)

テルペンインドールアルカロイド前駆体の生合成研究

2014

平成26年度、公益財団法人、福岡県すこやか健康事業団、がん研究助成金(奨励賞)

界面科学及び免疫化学的手法を基軸とした天然由来両親媒性物質の細胞膜上分子挙動解析

Grant number：25750167  2013 - 2015

科学研究費助成事業  若手研究(B)

生体膜モデルにおける天然由来食品添加物グリチルリチンの分子挙動解析

2013

平成25年度、第20回公益財団法人、日本食品化学研究振興財団、研究助成金

生体膜モデルにおける天然由来食品添加物グリチルリチンの分子挙動解析

2012

平成24年度、第19回公益財団法人、日本食品化学研究振興財団、研究助成金

Interfacial behavior of glycyrrhizin interacted with lipid rafts model by Langmuir monolayer study

2012

植物脂質ラフト形成機構の解明及びそのモデル構築と応用

Grant number：23810030  2011 - 2012

日本学術振興会  科学研究費助成事業  研究活動スタート支援

A fluorescent single domain antibody against plumbagin expressed in silkworm larvae for fluorescence-linked immunosorbent assay (Next-generation immunoassay using antibody)

2011

小型化抗体による植物二次代謝産物生合成メカニズムの解明と育種への応用

Grant number：09J00184  2009 - 2010

日本学術振興会  科学研究費助成事業  特別研究員奨励費

小型化抗体(scFv)を用いたELISA法の確立、及びその断片(VH, VL)を用いた新規高感度低分子分析法の開発研究

2008

平成20年度、日本科学協会、笹川科学研究助成金

抗プルンパギン(PL)組換え抗体を用いた新規高感度低分子分析法の開発研究

2008

平成20年度、財団法人九州大学後援会助成事業、学生の独創的研究に対する助成金

生薬学A

2024.4 - 2024.5   Spring quarter

漢方医薬学

2024.4 - 2024.5   Spring quarter

和漢医薬学

2023.10 - 2024.3   Second semester

薬学基礎実習II

2023.10 - 2024.3   Second semester

生物薬学演習I, II

2023.7   First semester

先端研究実験I, II

2023.4 - 2024.3   Full year

漢方医薬学

2023.4 - 2023.9   First semester

天然資源学

2023.4 - 2023.9   First semester

生薬学A

2023.4 - 2023.9   First semester

生薬学B

2023.4 - 2023.9   First semester

創薬科学総論I

2023.4 - 2023.9   First semester

身の回りの化学

2022.10 - 2023.3   Second semester

創薬科学総論III, IV

2022.10 - 2023.3   Second semester

和漢医薬学

2022.10 - 2023.3   Second semester

薬学基礎実習II

2022.10 - 2023.3   Second semester

先端研究実験I

2022.4 - 2023.3   Full year

先端研究実験II

2022.4 - 2023.3   Full year

生薬学A

2022.4 - 2022.9   First semester

天然資源学

2022.4 - 2022.9   First semester

生物薬学演習II

2022.4 - 2022.9   First semester

生物薬学演習I

2022.4 - 2022.9   First semester

薬学基礎実習II

2021.10 - 2022.3   Second semester

和漢医薬学

2021.10 - 2022.3   Second semester

先端研究実験I

2021.4 - 2022.3   Full year

先端研究実験II

2021.4 - 2022.3   Full year

薬学基礎実習II

2021.4 - 2021.9   First semester

天然資源学

2021.4 - 2021.9   First semester

生物薬学演習II

2021.4 - 2021.9   First semester

生物薬学演習I

2021.4 - 2021.9   First semester

生薬学A

2021.4 - 2021.9   First semester

創薬科学総論II

2021.4 - 2021.9   First semester

薬学基礎実習II

2020.10 - 2021.3   Second semester

先端研究実験I

2020.4 - 2021.3   Full year

先端研究実験II

2020.4 - 2021.3   Full year

和漢医薬学

2020.4 - 2020.9   First semester

天然資源学

2020.4 - 2020.9   First semester

生物薬学演習II

2020.4 - 2020.9   First semester

生物薬学演習I

2020.4 - 2020.9   First semester

生薬学A

2020.4 - 2020.9   First semester

先端研究実験I

2019.4 - 2020.3   Full year

先端研究実験II

2019.4 - 2020.3   Full year

生物薬学演習I

2019.4 - 2019.9   First semester

天然資源学

2019.4 - 2019.9   First semester

薬学基礎実習II

2019.4 - 2019.9   First semester

生物薬学演習II

2019.4 - 2019.9   First semester

先端研究実験I

2018.4 - 2019.3   Full year

先端研究実験II

2018.4 - 2019.3   Full year

生物薬学演習I

2018.4 - 2018.9   First semester

天然資源学

2018.4 - 2018.9   First semester

薬学基礎実習II

2018.4 - 2018.9   First semester

生物薬学演習II

2018.4 - 2018.9   First semester

先端研究実験I

2017.4 - 2018.3   Full year

先端研究実験II

2017.4 - 2018.3   Full year

薬学基礎実習II

2017.4 - 2017.9   First semester

生物薬学演習II

2017.4 - 2017.9   First semester

生物薬学演習I

2017.4 - 2017.9   First semester

天然資源学

2017.4 - 2017.9   First semester

自然科学総合実験

2016.10 - 2017.3   Second semester

先端研究実験I

2016.4 - 2017.3   Full year

先端研究実験II

2016.4 - 2017.3   Full year

薬学基礎実習II

2016.4 - 2016.9   First semester

生物薬学演習II

2016.4 - 2016.9   First semester

生物薬学演習I

2016.4 - 2016.9   First semester

天然資源学

2016.4 - 2016.9   First semester

自然科学総合実験

2015.10 - 2016.3   Second semester

先端研究実験I

2015.4 - 2016.3   Full year

先端研究実験II

2015.4 - 2016.3   Full year

薬学基礎実習II

2015.4 - 2015.9   First semester

医薬化学演習II

2015.4 - 2015.9   First semester

医薬化学演習I

2015.4 - 2015.9   First semester

生物薬学演習II

2015.4 - 2015.9   First semester

生物薬学演習I

2015.4 - 2015.9   First semester

天然資源学

2015.4 - 2015.9   First semester

先端研究実験I

2014.4 - 2015.3   Full year

先端研究実験II

2014.4 - 2015.3   Full year

薬学基礎実習II

2014.4 - 2014.9   First semester

医薬化学演習II

2014.4 - 2014.9   First semester

医薬化学演習I

2014.4 - 2014.9   First semester

生物薬学演習II

2014.4 - 2014.9   First semester

生物薬学演習I

2014.4 - 2014.9   First semester

天然資源学

2014.4 - 2014.9   First semester

2019.10   Role：Participation   Title：馬出地区　男女共同参画合同FD "「男女共同参画推進」や「ワークライフバランス推進」のために九州大学に求める今後の支援・施策等"

Organizer：[Undergraduate school/graduate school/graduate faculty]

2019.9   Role：Participation   Title：FD講演会 "外国人研究者・留学生受け入れ、貨物輸出と技術の提供に関して"

Organizer：[Undergraduate school/graduate school/graduate faculty]

2018.11   Role：Participation   Title：FD講演会 "研究分析ツール「SciVal」及び研究者プロファイリングツール「Pure」に関する説明会"

Organizer：[Undergraduate school/graduate school/graduate faculty]

2018.10   Role：Participation   Title：馬出地区　男女共同参画合同FD "医師のための働き方見直し～ワークライフバランスとダイバーシティの観点から～"

Organizer：[Undergraduate school/graduate school/graduate faculty]

2018.10   Role：Participation   Title：FD講演会 "M2B/Moodleに関するFD"

Organizer：[Undergraduate school/graduate school/graduate faculty]

2017.10   Role：Participation   Title：馬出地区　男女共同参画合同FD "無意識のバイアスからの開放：ダイバーシティのススメ"

Organizer：[Undergraduate school/graduate school/graduate faculty]

2017.3   Role：Participation   Title：FD講演会 "九州大学における情報セキュリティに関する取組"

Organizer：[Undergraduate school/graduate school/graduate faculty]

2016.10   Role：Participation   Title：馬出地区　男女共同参画合同FD "なぜ今「女性活躍推進法」か？－男女共同参画の必要性と九州大学における取組み－"

Organizer：[Undergraduate school/graduate school/graduate faculty]

2016.5   Role：Participation   Title：FD講演会 "外国人留学生の受入と管理（情報管理、輸出管理）について"

Organizer：[Undergraduate school/graduate school/graduate faculty]

2016.4   Role：Participation   Title：FD講演会 "Webを用いた電子ポートフォリオの使用方法の概要について"

Organizer：[Undergraduate school/graduate school/graduate faculty]

2015.6   Role：Participation   Title：FD講演会 "製薬業界の環境変化を受けた今後のＭＲ活動とメディカルアフェアーズの確立について"

Organizer：[Undergraduate school/graduate school/graduate faculty]

2015.2   Role：Participation   Title：FD講演会 "Education in Chemistry at Nanyang Technological University"

Organizer：[Undergraduate school/graduate school/graduate faculty]

大麻鑑定

Role(s)： Informant,　Investigater

警察機関  2023.4 - Present

漢方薬・生薬研修会　薬用植物園実習研修

日本薬剤師研修センター  薬学府附属薬用植物園  2022.10

令和4年度 九州大学大学院薬学研究院公開講座 「漢方および食の重要性について考える」

九州大学大学院薬学研究院  病院地区コラボステーションⅠ　２階視聴覚ホール  2022.10

漢方薬・生薬認定薬剤師　2021年度 試問実施

日本薬剤師研修センター  TKP　ガーデンシティ博多新幹線口  2022.8

漢方薬・生薬認定薬剤師　2020年度 試問実施

日本薬剤師研修センター  TKP　博多駅前シティーセンター  2022.1

大学体験講座「漢方薬とドーピング」

福岡県立伝習館高等学校  2021.11

漢方薬・生薬認定薬剤師　2019年度 試問実施

日本薬剤師研修センター  西鉄イン福岡  2020.11

漢方薬・生薬研修会　薬用植物園実習研修

日本薬剤師研修センター  薬学府附属薬用植物園  2019.10

漢方薬・生薬認定薬剤師　ビデオ集合研修

日本薬剤師研修センター  薬学研究院  2019.6

漢方薬・生薬認定薬剤師　2018年度 試問実施

日本薬剤師研修センター  薬学研究院  2019.6

漢方薬・生薬研修会　薬用植物園実習研修

日本薬剤師研修センター  薬学府附属薬用植物園  2019.5

漢方薬・生薬認定薬剤師　ビデオ集合研修

日本薬剤師研修センター  薬学研究院  2018.6

漢方薬・生薬認定薬剤師　2017年度 試問実施

日本薬剤師研修センター  薬学研究院  2018.6

漢方薬・生薬認定薬剤師　ビデオ集合研修

日本薬剤師研修センター  薬学研究院  2017.6

漢方薬・生薬認定薬剤師　2016年度 試問実施

日本薬剤師研修センター  薬学研究院  2017.6

漢方薬・生薬認定薬剤師　ビデオ集合研修

日本薬剤師研修センター  薬学研究院  2016.6

漢方薬・生薬認定薬剤師　2015年度 試問実施

日本薬剤師研修センター  薬学研究院  2016.6

漢方薬・生薬認定薬剤師　ビデオ集合研修

日本薬剤師研修センター  薬学研究院  2015.6