Updated on 2025/06/11

Information

 

写真a

 
NOGUCHI YASUNORI
 
Organization
Faculty of Pharmaceutical Sciences Department of Pharmaceutical Health Care and Sciences Assistant Professor
Graduate School of Pharmaceutical Sciences Department of Clinical Pharmacy（Concurrent）
School of Pharmaceutical Sciences Department of Clinical Pharmacy（Concurrent）
Title
Assistant Professor
Homepage
External link

Research Areas

  • Life Science / Molecular biology

Degree

  • Ph.D. in Pharmaceutical Sciences

Research History

  • Kyushu University Faculty of Pharmaceutical Sciences Department of Pharmaceutical Health Care and Sciences  Assistant Professor 

    2022.10 - Present

  • Imperial College London   

    2016.6 - 2022.9

  • Imperial College London   

Papers

  • The structural basis of Cdc7-Dbf4 kinase dependent targeting and phosphorylation of the MCM2-7 double hexamer Reviewed International journal

    Saleh, Almutasem*; Noguchi, Yasunori*; Aramayo, Ricardo; Ivanova, Marina E.; Stevens, Kathryn M.; Montoya, Alex; Sunidhi, S.; Carranza, Nicolas Lopez; Skwark, Marcin J.; Speck, Christian; *Contributed equally

    NATURE COMMUNICATIONS   13 ( 1 )   2022.5

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    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Nature Communications  

    The controlled assembly of replication forks is critical for genome stability. The Dbf4-dependent Cdc7 kinase (DDK) initiates replisome assembly by phosphorylating the MCM2-7 replicative helicase at the N-terminal tails of Mcm2, Mcm4 and Mcm6. At present, it remains poorly understood how DDK docks onto the helicase and how the kinase targets distal Mcm subunits for phosphorylation. Using cryo-electron microscopy and biochemical analysis we discovered that an interaction between the HBRCT domain of Dbf4 with Mcm2 serves as an anchoring point, which supports binding of DDK across the MCM2-7 double-hexamer interface and phosphorylation of Mcm4 on the opposite hexamer. Moreover, a rotation of DDK along its anchoring point allows phosphorylation of Mcm2 and Mcm6. In summary, our work provides fundamental insights into DDK structure, control and selective activation of the MCM2-7 helicase during DNA replication. Importantly, these insights can be exploited for development of novel DDK inhibitors.

    DOI： 10.1038/s41467-022-30576-1

    Scopus

    Repository Public URL： https://hdl.handle.net/2324/7161497

  • The structure of ORC-Cdc6 on an origin DNA reveals the mechanism of ORC activation by the replication initiator Cdc6 Reviewed International journal

    Feng, Xiang; Noguchi, Yasunori; Barbon, Marta; Stillman, Bruce; Speck, Christian; Li, Huilin

    NATURE COMMUNICATIONS   12 ( 1 )   2021.6

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41467-021-24199-1

  • Regulatory dynamics in the ternary DnaA complex for initiation of chromosomal replication in Escherichia coli Invited Reviewed International journal

    Sakiyama, Yukari; Kasho, Kazutoshi; Noguchi, Yasunori; Kawakami, Hironori; Katayama, Tsutomu

    NUCLEIC ACIDS RESEARCH   45 ( 21 )   12354 - 12373   2017.12

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/nar/gkx914

  • Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion model Reviewed International journal

    Noguchi, Yasunori; Yuan, Zuanning; Bai, Lin; Schneider, Sarah; Zhao, Gongpu; Stillman, Bruce; Speck, Christian; Li, Huilin

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   114 ( 45 )   E9529 - E9538   2017.11

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    DOI： 10.1073/pnas.1712537114

  • Near-atomic structural model for bacterial DNA replication initiation complex and its functional insights Reviewed International journal

    Shimizu, Masahiro; Noguchi, Yasunori; Sakiyama, Yukari; Kawakami, Hironori; Katayama, Tsutomu; Takada, Shoji

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   113 ( 50 )   E8021 - E8030   2016.12

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    DOI： 10.1073/pnas.1609649113

  • The Escherichia coli Cryptic Prophage Protein YfdR Binds to DnaA and Initiation of Chromosomal Replication Is Inhibited by Overexpression of the Gene Cluster yfdQ-yfdR-yfdS-yfdT Reviewed International journal

    Noguchi, Yasunori; Katayama, Tsutomu

    FRONTIERS IN MICROBIOLOGY   7   2016.3

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    DOI： 10.3389/fmicb.2016.00239

  • The Arg Fingers of Key DnaA Protomers Are Oriented Inward within the Replication Origin oriC and Stimulate DnaA Subcomplexes in the Initiation Complex Reviewed International journal

    Noguchi, Yasunori; Sakiyama, Yukari; Kawakami, Hironori; Katayama, Tsutomu

    JOURNAL OF BIOLOGICAL CHEMISTRY   290 ( 33 )   20295 - 20312   2015.8

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    DOI： 10.1074/jbc.M115.662601

  • A Replicase Clamp-Binding Dynamin-like Protein Promotes Colocalization of Nascent DNA Strands and Equipartitioning of Chromosomes in E. coli Reviewed International journal

    Ozaki, Shogo; Matsuda, Yusaku; Keyamura, Kenji; Kawakami, Hironori; Noguchi, Yasunori; Kasho, Kazutoshi; Nagata, Komomo; Masuda, Tamami; Sakiyama, Yukari; Katayama, Tsutomu

    CELL REPORTS   4 ( 5 )   985 - 995   2013.9

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.celrep.2013.07.040

  • Differentiation of the DnaA-oriC subcomplex for DNA unwinding in a replication initiation complex Reviewed International journal

    Shogo Ozaki, Yasunori Noguchi, Yasuhisa Hayashi, Erika Miyazaki, Tsutomu Katayama

    Journal of Biological Chemistry   287 ( 44 )   37458 - 37471   2012.10

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    DOI： 10.1074/jbc.M112.372052

  • Stable nucleotide binding to DnaA requires a specific glutamic acid residue within the AAA+ box II motif Reviewed International journal

    Shogo Ozaki, Yasunori Noguchi, Masahiro Nishimura, Tsutomu Katayama

    Journal of Structural Biology   179 ( 2 )   242 - 250   2012.8

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jsb.2012.05.001

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Presentations

  • 生化学的解析によるヒトDNA複製開始の分子機構の解明 Invited

    第47回日本分子生物学会年会  2024.11 

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    Event date： 2024.11

    Language：English   Presentation type：Oral presentation (invited, special)  

  • 真核生物における複製ヘリカーゼ形成の分子機構の解明 Invited

    日本遺伝学会第96回大会  2024.9 

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    Event date： 2024.9

  • Molecular mechanism of the DDK-dependent MCM2-7 activation Invited

    Yasunori Noguchi, Almutasem Saleh, Sarah Schneider, Masatoshi Fujita and Christian Speck  

    第96回日本生化学会大会  2023.11 

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    Event date： 2023.10 - 2024.11

    Language：English  

    Country：Japan  

  • DNA複製開始におけるMCM2-7活性化の分子機構 Invited

    野口　泰徳

    国⽴遺伝学研究所研究会「染⾊体安定維持研究会」  2023.7 

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    Event date： 2023.7

    Language：Japanese  

    Country：Japan  

  • Molecular mechanism of the MCM2-7 activation by DDK International conference

    Yasunori Noguchi, Almutasem Saleh, Sarah Schneider, and Marina Ivanova, and Christian Speck

    NIG International Symposium 2022, Chromosome Replication in the New Era; Old and New Questions in Life Science  2022.11 

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    Event date： 2022.11

    Language：English  

    Country：Japan  

  • 染色体DNA複製開始複合体と開始制御メカニズムの新たな展望　DDK依存的なMCM2-7活性化の分子機序(Molecular mechanism of the DDK-dependent MCM2-7 activation)

    野口 泰徳, Almutasem Saleh, Sarah Schneider, 藤田 雅俊, Christian Speck

    日本生化学会大会プログラム・講演要旨集  2023.10  (公社)日本生化学会

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    Language：English  

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MISC

  • From structure to mechanism-understanding initiation of DNA replication Reviewed

    Alberto Riera, Marta Barbon, Yasunori Noguchi, L Maximilian Reuter, Sarah Schneider, Christian Speck

    2017.6

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Professional Memberships

  • 日本生化学会

  • 日本分子生物学会

Research Projects

  • ヒト細胞DNA複製メカニズムの 解明

    2023.4 - 2024.3

    Joint research

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    Authorship：Principal investigator  Grant type：Other funds from industry-academia collaboration

  • ヒト細胞系におけるDDKキナーゼ依存的なMCM2-7ヘリカーゼの活性化機構の解明

    Grant number：23K05639  2023 - 2025

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    野口 泰徳

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    Authorship：Principal investigator  Grant type：Scientific research funding

    DNAヘリカーゼであるMCM2-7の細胞周期に依存した適時的な活性化がDNA複製開始には重要である。DDKキナーゼはMCM2-7の活性化反応において中心的な役割を担う。代表者はこれまでに出芽酵母タンパク質を用いた生化学的解析により、DNA複製開始におけるDDKの機能を明らかにした。しかしながら、ヒトDDKによるMCM2-7活性化の分子機構はよくわかっていない。本研究では、生化学的解析によって、ヒトDDKによる複製開始制御の分子機構を明らかにする。

    CiNii Research

Class subject

  • 染色体生物学とそのがん研究への応用

    2025.4 - 2025.6   Spring quarter

  • 生物薬学研究

    2024.11   Fall quarter

  • 染色体生物学とそのがん研究への応用

    2024.4 - 2024.6   Spring quarter

  • 染色体生物学とそのがん研究への応用

    2023.4 - 2023.6   Spring quarter

FD Participation

  • 2024.11   Role：Participation   Title：第6回創薬産学官連携セミナー（アカデミア創薬）

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2024.7   Role：Participation   Title：第5回創薬産学官連携セミナー（新モダリティ）

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2023.11   Role：Participation   Title：第２回部局FD講演会「機関間連携」

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2023.8   Role：Participation   Title：令和５年度４部局合同男女共同参画ＦＤ

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2023.4   Role：Participation   Title：令和５年度 第１回全学ＦＤ（新任教員の研修）

    Organizer：University-wide

  • 2022.11   Role：Participation   Title：第４回創薬産学官連携セミナー（アカデミア創薬）

    Organizer：[Undergraduate school/graduate school/graduate faculty]

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