Updated on 2025/06/09

写真a

 
YOSHINORI KAWABE
 
Organization
Faculty of Engineering Department of Chemical Engineering Associate Professor
School of Engineering (Concurrent)
Graduate School of Engineering (Concurrent)
Title
Associate Professor
Contact information
メールアドレス
Tel
0928022753
Profile
研究活動として、効率的なトランスジェニック鳥類作製技術の開発とバイオ医薬品の大量生産に関する研究を行い、研究室の大学院生および学部生に対して修士論文および卒業論文の研究指導をおこなっている。 教育活動として、学部生に対してのコアセミナーや学生実験を担当している。

Research Areas

  • Life Science / Biomaterials

  • Life Science / Biomedical engineering

  • Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering

Degree

  • Doctor of Engineering, Nagoya University, Japan ( 2006.9 Nagoya University )

Research History

  • Kyushu University Department of Chemical Engineering, Faculty of Engineering Associate Professor 

    2020.2 - Present

      More details

    Country:Japan

    researchmap

  • Kyushu University  Assistant Professor 

    2007.4 - 2020.1

      More details

    Country:Japan

  • University of Miyazaki 工学部 非常勤講師 

    2022.4 - Present

      More details

    Country:Japan

    researchmap

Education

  • Nagoya University   大学院工学研究科   化学・生物工学専攻

    2004.4 - 2006.9

      More details

    Country:Japan

  • Nagoya University   大学院工学研究科   生物機能工学専攻

    2002.4 - 2004.3

      More details

    Country:Japan

  • Nagoya University   工学部   化学・生物工学科

    1998.4 - 2002.3

      More details

    Country:Japan

Research Interests・Research Keywords

  • Research theme: Development and application of microalgae biotechnology

    Keyword: Algae, Microalgae, Chlamydomonas reinhardtii, transgene integration, gene retargeting, recombinant protein production

    Research period: 2017.4

  • Research theme: development of maintainance and efficient derivation for pluripotential stem cells

    Keyword: pluripotential stem cells, genetically manipulated animals, feeder layer, tissue engineering

    Research period: 2008.4

  • Research theme: Mass production sysytem for the biopharmaceutical proteins by transgenic avian bioreactor

    Keyword: transgenic avian bioreactor, biopharmaceuticals, recombinant proteins, monoclonal antibody, retroviral vector

    Research period: 2004.4

Awards

  • 第24回生物工学論文賞

    2016.9   日本生物工学会  

  • 第27回(平成27年度)加藤記念国際交流助成

    2015.3   加藤記念バイオサイエンス振興財団  

  • 日本動物細胞工学会奨励賞

    2013.7   日本動物細胞工学会   医薬品タンパク質生産技術の開発

  • 第17回生物工学論文賞

    2009.9   日本生物工学会  

  • 第15回生物工学論文賞

    2007.9   日本生物工学会  

Papers

  • Preparation of minicircle DNA vectors for gene knock-in to mammalian cells Reviewed

    Yoshinori Kawabe, Makoto Hamaoka, Akio Kuno, Yusaku Yoshimura, Masamichi Kamihira

    Journal of Bioscience and Bioengineering   in press   2025

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jbiosc.2025.05.005

  • Inducible transgene expression in CHO cells using an artificial transcriptional activator with estrogen-binding domain Reviewed

    Md Rashidur Rahman, Yoshinori Kawabe, Kozumi Suzuki, Satoshi Chen, Yuki Amamoto, Masamichi Kamihira

    Biotechnology Journal   19 ( 1 )   e2300362   2024.1   ISSN:18606768 eISSN:18607314

     More details

    Language:English   Publisher:Biotechnology Journal  

    Biopharmaceuticals, including therapeutic antibodies, are rapidly growing products in the pharmaceutical market. Mammalian cells, such as Chinese hamster ovary (CHO) cells, are widely used as production hosts because recombinant antibodies require complex three-dimensional structures modified with sugar chains. Recombinant protein production using mammalian cells is generally performed with cell growth. In this study, we developed a technology that controls cell growth and recombinant protein production to induce recombinant protein production with predetermined timing. Expression of green fluorescent protein (GFP) gene and a single-chain antibody fused with the Fc-region of the human IgG1 (scFv-Fc) gene can be induced and mediated by the estrogen receptor-based artificial transcription factor Gal4-ERT2-VP16 and corresponding inducer drugs. We generated CHO cells using an artificial gene expression system. The addition of various concentrations of inducer drugs to the culture medium allowed control of proliferation and transgene expression of the engineered CHO cells. Use of 4-hydroxytamoxifen, an antagonist of estrogen, as an inducing agent yielded high gene expression at a concentration more than 10-fold lower than that of β-estradiol. When scFv-Fc was produced under inducing conditions, continuous production was possible for more than 2 weeks while maintaining high specific productivity (57 pg cell−1 day−1). This artificial gene expression control system that utilizes the estrogen response of estrogen receptors can be an effective method for inducible production of biopharmaceuticals.

    DOI: 10.1002/biot.202300362

    Scopus

    PubMed

    CiNii Research

    researchmap

  • High-Level Production of scFv-Fc Antibody Using an Artificial Promoter System with Transcriptional Positive Feedback Loop of Transactivator in CHO Cells

    Binbin Ying, Yoshinori Kawabe, Feiyang Zheng, Yuki Amamoto, Masamichi Kamihira

    Cells   12 ( 22 )   2638 - 2638   2023.11   eISSN:20734409

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    With the increasing demand for therapeutic antibodies, CHO cells have become the de facto standard as producer host cells for biopharmaceutical production. High production yields are required for antibody production, and developing a high-titer production system is increasingly crucial. This study was established to develop a high-production system using a synthetic biology approach by designing a gene expression system based on an artificial transcription factor that can strongly induce the high expression of target genes in CHO cells. To demonstrate the functionality of this artificial gene expression system and its ability to induce the high expression of target genes in CHO cells, a model antibody (scFv-Fc) was produced using this system. Excellent results were obtained with the plate scale, and when attempting continuous production in semi-continuous cultures using bioreactor tubes with high-cell-density suspension culture using a serum-free medium, high-titer antibody production at the gram-per-liter level was achieved. Shifting the culture temperature to a low temperature of 33 °C achieved scFv-Fc concentrations of up to 5.5 g/L with a specific production rate of 262 pg/(cell∙day). This artificial gene expression system should be a powerful tool for CHO cell engineering aimed at constructing high-yield production systems.

    DOI: 10.3390/cells12222638

    Scopus

    PubMed

    CiNii Research

    researchmap

  • Novel cell lines derived from Chinese hamster kidney tissue Reviewed

    Yoshinori Kawabe, Masamichi Kamihira

    PLoS One   17 ( 3 )   e0266061   2022.3

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pone.0266061

    Other Link: https://pmc.ncbi.nlm.nih.gov/articles/PMC8970510/

  • Novel transgenic Chlamydomonas reinhardtii strain with retargetable genomic transgene integration using Cre-loxP system

    Guan Huang, Yoshinori Kawabe, Kazuki Shirakawa, Tatsuki Akiyama, Masamichi Kamihira

    Journal of Bioscience and Bioengineering   132 ( 5 )   469 - 478   2021.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jbiosc.2021.07.006

▼display all

Books

  • Production of Antibody by Transgenic Avians. In: Al-Rubeai, M. (eds) Antibody Expression and Production. Cell Engineering, vol 7. Springer, Dordrecht.

    Yoshinori Kawabe, Masamichi Kamihira(Role:Joint author)

    2011.4 

     More details

    Responsible for pages:pp121-141   Language:English   Book type:General book, introductory book for general audience

    DOI: https://doi.org/10.1007/978-94-007-1257-7_6

    Other Link: https://link.springer.com/chapter/10.1007/978-94-007-1257-7_6

  • 微細藻類スマートグリーンセルファクトリーの創生と展望

    河邉佳典(Role:Contributorゲノム編集の最新技術と医薬品・遺伝子治療・農業・水畜産物・有用物質生産への活用)

    技術情報協会  2023.8    ISBN:9784861049781

     More details

  • 浮遊培養による動物細胞の培養

    河邉佳典(Role:Contributorバイオプロセスにおける 培養・分離技術と物質生産への応用)

    技術情報協会  2022.10    ISBN:9784861049040

     More details

  • バイオ医薬品高生産細胞株構築のためのゲノム操作技術

    河邉佳典, 上平正道

    月刊ファームステージ 17(4):31-36  2017.7 

     More details

    Language:Others  

  • Cre-loxPシステムを用いたCHO細胞ゲノムへの改良された遺伝子導入

    稲生崇規, 河邉佳典, 山城拓郎, 亀山雄二郎, 汪雪, 井藤彰, 上平正道

    生物工学会誌 第95巻第2号74頁  2017.2 

     More details

    Language:Others  

▼display all

Presentations

  • Microalgae cell engineering technology toward sustainable green cell factories Invited International conference

    Yoshinori Kawabe

    Japan-Croatia Joint Symposium on Chemical Engineering & Plasma Agriculture for Young Scientists Global Networking  2024.10 

     More details

    Event date: 2024.10

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Croatia  

  • [S1-01] Generation and application of transgenic chickens towards robust production system of recombinant proteins Invited International conference

    @Yoshinori Kawabe, @Ken-ichi Nishijima, @Masamichi Kamihira

    JAACT2023Nagoya  2023.11 

     More details

    Event date: 2023.11 - 2023.12

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Nagoya Congress Center   Country:Japan  

  • [J122] 微細藻類クラミドモナスでの人工転写活性化因子による外来遺伝子発現システム

    @河邉 佳典、#秋山 立幹、#宮副 こころ、@上平 正道

    化学工学会第88年会(東京)  2023.3 

     More details

    Event date: 2023.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京農工大学小金井キャンパス・GOING VIRTUAL   Country:Japan  

  • [I206] 人工遺伝子発現誘導システムを有するCHK細胞の組換え抗体生産評価

    @河邉 佳典、#境 紘洋、#巖 流征、@上平 正道

    化学工学会第87年会  2022.3 

     More details

    Event date: 2022.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:神戸大学・化学工学会GOING VIRTUAL   Country:Japan  

  • [VC313] ゲノム操作技術を用いた有用物質生産のためのセルエンジニアリング Invited

    @河邉 佳典

    化学工学会 第52回秋季大会 (2021)  2021.9 

     More details

    Event date: 2021.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岡山大学   Country:Japan  

▼display all

MISC

  • 第4章「農業分野におけるゲノム編集技術の活用と課題対策」第8節「微細藻類スマートグリーンセルファクトリーの創生と展望」

    @河邉佳典

    ゲノム編集の最新技術と 医薬品・遺伝子治療・農業・水畜産物・有用物質生産への活用、技術情報協会   2023.8

     More details

    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • 第2章「動物細胞培養での留意点と物質生産への応用」 第2節「浮遊培養による動物細胞の培養」

    @河邉 佳典

    バイオプロセスを用いた有用性物質生産技術、技術情報協会   2022.11

     More details

    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • 第4章「ゲノム編集によるスマートセルインダストリーの技術開発とその課題解決」 第2節「ゲノム編集を用いたタンパク質の高生産細胞株の作製とバイオ医薬生産への応用」

    @河邉佳典、@上平正道、

    「ゲノム編集技術を応用した製品開発とその実用化」(株)技術情報協会   2021.2

     More details

    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • バイオ医薬品高生産細胞株構築のためのゲノム操作技術

    河邉 佳典, 上平 正道

    月刊ファームステージ   2017.7

     More details

    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  • 特集 産業応用を指向した細胞の操作・計測技術 「ゲノム操作工学によるバイオ医薬品生産技術の開発」

    河邉 佳典, 上平 正道

    生物工学会誌 – 94巻9号   2016.9

     More details

    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

▼display all

Works

  • [Chlamydomonas Resource Center (CC-5888)] Chlamy/S

    Yoshinori Kawabe, Masamichi Kamihira

    2022.4 - Present

     More details

Industrial property rights

Patent   Number of applications: 3   Number of registrations: 0
Utility model   Number of applications: 0   Number of registrations: 0
Design   Number of applications: 0   Number of registrations: 0
Trademark   Number of applications: 0   Number of registrations: 0

Professional Memberships

  • The Molecular Biology Society of Japan

  • Japanese Association for Animal Cell Technology

  • The Society of Chemical Engineers, Japan

  • The Society of Biotechnology, Japan

  • The Society for Biotechnology, Japan

      More details

▼display all

Committee Memberships

  • 日本動物細胞工学会   評議員   Domestic

    2025 - 2026   

      More details

    Committee type:Academic society

  • 化学工学会   代議員   Domestic

    2025 - 2026   

      More details

    Committee type:Academic society

  • 化学工学会   代議員   Domestic

    2024.5 - 2025.4   

  • 化学工学会   北九州化学工学懇話会(KACE)幹事   Domestic

    2024.4 - 2026.3   

  • 化学工学会   国際交流センター韓国委員会委員   Domestic

    2023.4 - 2025.4   

▼display all

Academic Activities

  • 実行委員会委員

    第26回化学工学会学生発表会  ( Japan ) 2024.3

     More details

    Type:Competition, symposium, etc. 

  • 座長

    第26回化学工学会学生発表会  ( Japan ) 2024.3

     More details

    Type:Competition, symposium, etc. 

  • Chairperson International contribution

    Japanese Association for Animal Cell Technology (JAACT)2023Nagoya  ( Nagoya Congress center Japan ) 2023.11 - 2023.12

     More details

    Type:Competition, symposium, etc. 

  • とりまとめ

    2023年度化学工学会バイオ部会インフォーマルミーティング  ( Japan ) 2023.9

     More details

    Type:Competition, symposium, etc. 

  • 座長

    第75回日本生物工学会大会  ( Japan ) 2023.9

     More details

    Type:Competition, symposium, etc. 

▼display all

Research Projects

  • .

    2021

    令和3年度QRプログラム

      More details

    Authorship:Principal investigator  Grant type:On-campus funds, funds, etc.

  • Development of integrated animal cell engineering system for biopharmaceutical production

    Grant number:20H00322  2020 - 2023

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

    Kamihira Masamichi

      More details

    Authorship:Coinvestigator(s)  Grant type:Scientific research funding

    Based on the gene engineering technology for animal cells that we have developed, we have constructed a cell engineering cycle for the production of functional cells used in biopharmaceutical production and cellular medicines. This has enabled the systematization of functional modification of animal cells. Additionally, as part of the development of original elementary technologies in cell engineering, we have worked on developing new host cells, creating artificial gene expression control systems, producing transgenic chickens using primordial germ cells, and advancing embryo manipulation technology to generate transgenic chickens. We have applied these efforts to the production of biopharmaceuticals and the creation of functional cells, making it possible to design and create cells and chickens with specific functions.

    CiNii Research

  • Generation of engineered designer microalgae by using a genome manipulation technology

    Grant number:20K05233  2020 - 2022

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Kawabe Yoshinori

      More details

    Authorship:Principal investigator  Grant type:Scientific research funding

    In recent years, microalgae have attracted a great deal of attention as a host cell for the bioeconomy toward the realization of a carbon neutral society. Development of on-genome/gene manipulation techniques will facilitate the productivity of useful substance. In this study, we developed a genetically modified microalgae (Chlamydomonas) cell line that can stably express foreign genes at high levels throughout the culture period. In addition, we constructed an artificial gene expression system for the purpose of developing a higher expression system for Chlamydomonas. We developed an artificial transcription factor suitable for microalgae, and found an artificial synthetic promoter suitable for it.

    CiNii Research

  • 次世代治療・診断実現のための創薬基盤技術開発事業 2.バイオ医薬品の高度製造技術の開発 2-5.先端的バイオ製造技術開発 「増殖-分化制御システムを取り入れたCHK細胞を用いたバイオ医薬品生産細胞の構築」

    2018 - 2020

    日本医療研究開発機構(AMED)(内閣府)

      More details

    Authorship:Principal investigator  Grant type:Contract research

  • 遺伝子改変自由自在な次世代型微細藻類バイオセルファクトリーの創製

    2017 - 2018

    平成29年度工学研究新分野開拓助成

      More details

    Authorship:Principal investigator  Grant type:On-campus funds, funds, etc.

▼display all

Class subject

  • 生物化学工学

    2024.12 - 2025.3   Winter quarter

  • 化学工学実験第三

    2024.10 - 2025.3   Second semester

  • 基礎生命工学

    2024.10 - 2024.12   Fall quarter

  • 化学工学B

    2024.10 - 2024.12   Fall quarter

  • 生物機能システム工学

    2024.6 - 2024.8   Summer quarter

▼display all

FD Participation

  • 2022.9   Role:Participation   Title:2022年度 課題協学科目 第2回FD

    Organizer:University-wide

  • 2022.6   Role:Participation   Title:2022年度 課題協学科目 第1回FD

    Organizer:University-wide

  • 2021.11   Role:Participation   Title:令和3年度化学工学部門FD「大学の授業における合理的配慮」

    Organizer:Undergraduate school department

  • 2020.10   Role:Participation   Title:第1回工学部FD「総合型選抜の実施に向けて–評価基準の策定–」

    Organizer:[Undergraduate school/graduate school/graduate faculty]

  • 2020.5   Role:Participation   Title:オンサイト授業 vs. オンライン授業:分かったこと,変わったこと

    Organizer:[Undergraduate school/graduate school/graduate faculty]

▼display all

Visiting, concurrent, or part-time lecturers at other universities, institutions, etc.

  • 2025  宮崎大学・工学部  Classification:Intensive course  Domestic/International Classification:Japan 

  • 2024  宮崎大学・工学部  Classification:Part-time lecturer  Domestic/International Classification:Japan 

  • 2023  宮崎大学・工学部  Classification:Part-time lecturer  Domestic/International Classification:Japan 

  • 2022  宮崎大学・工学部  Classification:Part-time lecturer  Domestic/International Classification:Japan 

  • 2016  九州大学 日韓共同理工系学部留学生予備教育コース 学内非常勤講師  Classification:Part-time lecturer  Domestic/International Classification:Japan 

Participation in international educational events, etc.

  • 2016.1

    九州大学

    English Learning in Teaching English (ELITE)九大研修・講習会

      More details

    Venue:日本・福岡

Other educational activity and Special note

  • 2025  Class Teacher  化学工学科2年生

  • 2024  Class Teacher  学部

  • 2023  Coaching of Students' Association  秋期工場見学

     詳細を見る

    化学工学科3年生の引率

  • 2022  Class Teacher  学部

  • 2021  Class Teacher  学部

▼display all

Social Activities

  • オープンキャンパス(化学工学科とりまとめ)

    Role(s):Presenter, Organizing member

    2024.8

  • オープンキャンパス研究室紹介

    2023.8

     More details

    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

  • オープンキャンパス(模擬講義)「生物の巧みな機能で挑戦する化学と工学」

    2022.8

     More details

    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

  • オープンキャンパス(模擬講義)「生物の巧みな機能で挑戦する化学と工学」

    Role(s):Lecturer

    九州大学工学部化学工学科  2022.8

     More details

    Type:Visiting lecture

    researchmap

  • 出前講義「最先端バイオテクノロジーがひらく世界」

    熊本県立済々黌高等学校  2020.11

     More details

    Audience:Infants, Schoolchildren, Junior students, High school students

    Type:Seminar, workshop

▼display all

Acceptance of Foreign Researchers, etc.

  • IIT Kanpur

    Acceptance period: 2012.3   (Period):2weeks to less than 1 month

    Nationality:India

    Business entity:Japan Society for the Promotion of Science

  • IIT Kanpur

    Acceptance period: 2010.12 - 2011.1  

    Nationality:India

    Business entity:Japan Society for the Promotion of Science

  • IIT Kanpur

    Acceptance period: 2008.11 - 2008.12   (Period):Less than 2 weeks

    Nationality:India

    Business entity:Japan Society for the Promotion of Science

  • IIT Kanpur

    Acceptance period: 2008.4   (Period):Less than 2 weeks

    Nationality:India

    Business entity:Japan Society for the Promotion of Science

Travel Abroad

  • 2024.10

    Staying countory name 1:Croatia   Staying institution name 1:University of Zagreb (Japan-Croatia Joint Symposium on Chemical Engineering & Plasma Agriculture for Young Scientists Global Networking)

  • 2019.5

    Staying countory name 1:Sweden   Staying institution name 1:Lund University

  • 2019.5

    Staying countory name 1:Denmark   Staying institution name 1:Bella Center, Copenhagen Congress Center (ESACT2019)

  • 2018.11

    Staying countory name 1:Korea, Republic of   Staying institution name 1:Yeungnam University (The 5th SKY Joint Symposium)

  • 2017.5

    Staying countory name 1:Switzerland   Staying institution name 1:SwissTech Convention Center at Lausanne (ESACT2017)

▼display all