Updated on 2024/10/31

Information

 

写真a

 
SONOI RIE
 
Organization
Faculty of Engineering Department of Chemical Engineering Assistant Professor
School of Engineering （Concurrent）
Graduate School of Engineering （Concurrent）
Title
Assistant Professor
Contact information
メールアドレス
Tel
0928022805
Profile
Establishment of non-invasive and quantitative evaluation methods based on cell characteristics and behaviors
External link

Degree

  • Doctor of Philosophy in Engineering, Osaka University

Research History

  • Cell Exosome Therapeutics株式会社　細胞培養加工施設管理業務

    Cell Exosome Therapeutics株式会社

    2021.4 - 2021.6

  • 2014年4月　 大阪大学工学研究科　特任研究員 2015年5月　 大阪大学医学系研究科　特任研究員 2017年4月　 日本医科大学　ポストドクター 2017年10月　九州大学病院　細胞培養加工施設技術職員 2018年7月　 産業技術総合研究所　産総研特別研究員 2021年9月　 理化学研究所　研究員 2022年5月　 金沢大学　ナノ生命科学研究所　博士研究員 2022年8月　 物質・材料研究機構　NIMSポスドク研究員

Research Interests・Research Keywords

  • Research theme：A novel strategy to facilitate uniform epithelial cell maturation using liquid–liquid interfaces

    Keyword：liquid-liquid interfaces，epithelial cell, uniform maturation, tight junction, cell behavior

    Research period： 2023.4 - 2025.3

  • Research theme：１．Study on quantitative evaluation and regulation of the maturation through the migratory behaviors of human retinal pigment epithelial cells in confluent state (2011-2017). ２．Study on the development of process and quality control technology for inducing differentiation of human iPS cells into retinal pigment epithelial cells for use in the transplantation (2014-2015). ３．Study on the development of quantitative and non-invasive evaluation methods to understand drug toxicity in HepaRG aggregates using Optical Coherence Tomography (OCT) (2018-2021). ４．Study on establishment of quantitative and non-invasive evaluation methods to understand drug toxicity based on the change in the bile canaliculi behaviors of Hepatocytes (2018-2022).

    Keyword：confluent state, epithelial cells, non-invasive evaluation, image analysis, regenerative medicine, drug discovery

    Research period： 2023.1

Awards

  • 化学工学会第44回秋季大会，バイオ部会優秀ポスター賞

    2012.9   化学工学会バイオ部会  

Papers

  • A novel strategy to facilitate uniform epithelial cell maturation using liquid–liquid interfaces Reviewed International journal

    2024.5

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Epithelial tissue forms and maintains a critical barrier function in the body. A novel culture design aimed at promoting uniform maturation of epithelial cells using liquid materials is described. Culturing Madin–Darby canine kidney (MDCK) cells at the liquid–liquid interface yielded reduced migration and stimulated active cell growth. Similar to solid–liquid interfaces, cells cultured on a fibronectin-coated liquid–liquid interface exhibited active migration and growth, ultimately reaching a confluent state. These cells exhibited reduced stress fiber formation and adopted a cobblestone-like shape, which led to their even distribution in the culture vessel. To inhibit stress fiber formation and apoptosis, the exposure of cells on liquid–liquid interfaces to Y27632, a specific inhibitor of the Rho-associated protein kinase (ROCK), facilitated tight junction formation (frequency of ZO-2-positive cells, FZ = 0.73). In Y27632-exposed cells on the liquid–liquid interface, the value obtained by subtracting the standard deviation of the ratio of nucleus densities in each region that compartmentalized a culture vessel from 1, denoted as HLN, was 0.93 ± 0.01, indicated even cell distribution in the culture vessel at t = 72 h. The behavior of epithelial cells on liquid–liquid interfaces contributes to the promotion of their uniform maturation.

    Other Link： https://www.nature.com/articles/s41598-024-63115-7

    Repository Public URL： https://hdl.handle.net/2324/7178836

  • Quantitative understanding of HepaRG cells during drug‐induced intrahepatic cholestasis through changes in bile canaliculi dynamics Reviewed International journal

    Sonoi R., Hagihara Y.

    Pharmacology Research and Perspectives   10 ( 3 )   2022.5

    　More details

    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Pharmacology Research and Perspectives  

    An understanding of the quantitative relationship between bile canaliculus (BC) dynamics and the disruption of tight junctions (TJs) during drug-induced intrahepatic cholestasis may lead to new strategies aimed at drug development and toxicity testing. To investigate the relationship between BC dynamics and TJ disruption, we retrospectively analyzed the extent of TJ disruption in response to changes in the dynamics of BCs cultured with entacapone (ENT). Three hours after adding ENT, the ZO-1-negative BC surface area ratio became significantly higher (4.1-fold) than those of ZO-1-positive BCs. Based on these data, we calculated slopes of surface area changes, m, of each ZO-1-positive and ZO-1-negative BC. BCs with m ≤ 15 that fell within the 95% confidence interval of ZO-1-positive BCs were defined as ZO-1-positive. To validate this method, we compared the frequency of ZO-1-positive BCs, FZ, with that of BCs with m ≤ 15, FT, in culture using drugs that regulate TJ, or induce intrahepatic cholestasis. FT values were correlated with FZ under all culture conditions (R2 =.99). Our results indicate that the magnitude of BC surface area changes is a factor affecting TJ disruption, suggesting that maintaining TJ integrity by slowing BC dilation inhibits cell death.

    DOI： 10.1002/prp2.960

    Scopus

    Repository Public URL： https://hdl.handle.net/2324/7172695

  • Tight junction stabilization prevents HepaRG cell death in drug-induced intrahepatic cholestasis Reviewed International journal

    Sonoi, R. and Hagihara Y.

    Biology Open   2021.6

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1242/bio.058606

    Repository Public URL： https://hdl.handle.net/2324/7172697

  • Noninvasive Evaluation of HepaRG Aggregates during Drug‐Induced Intrahepatic Cholestasis Using Optical Coherence Tomography Reviewed International journal

    5 ( 2 )   2021.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/adbi.202000198

    Repository Public URL： https://hdl.handle.net/2324/7172698

  • Switching of cell fate through the regulation of cell growth during drug-induced intrahepatic cholestasis Reviewed International journal

    Sonoi, R., and Hagihara, Y.

    Journal of Bioscience and Bioengineering   130 ( 6 )   659 - 665   2020.12

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jbiosc.2020.08.004

    Repository Public URL： https://hdl.handle.net/2324/7172699

  • Phenotypic heterogeneity of human retinal pigment epithelial cells in passaged cell populations Reviewed International journal

    Sonoi, R., Kim, M.H., Yamada, K., and Kino-oka, M.

    Journal of bioscience and bioengineering   124 ( 2 )   227 - 233   2017.8

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jbiosc.2017.03.008

    Repository Public URL： https://hdl.handle.net/2324/7172694

  • Facilitation of uniform maturation of human retinal pigment epithelial cells through collective movement in culture Reviewed International journal

    Sonoi, R., Kim, M.H., and Kino-oka, M.

    Journal of bioscience and bioengineering   121 ( 2 )   220 - 226   2016.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jbiosc.2015.05.019

    Repository Public URL： https://hdl.handle.net/2324/7172692

  • Locational heterogeneity of maturation by changes in migratory behaviors of human retinal pigment epithelial cells in culture Reviewed International journal

    Sonoi, R., Kim, M.H., and Kino-oka, M.

    Journal of bioscience and bioengineering   119 ( 1 )   107 - 112   2015.1

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jbiosc.2014.05.025

    Repository Public URL： https://hdl.handle.net/2324/7172690

  • Analysis of locality of early-stage maturation in confluent state of human retinal pigment epithelial cells Reviewed International journal

    Kim, M.H., Sonoi, R., Yamada, K., Inamori, M., and Kino-oka, M.

    Journal of bioscience and bioengineering   113 ( 6 )   778 - 781   2012.6

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jbiosc.2012.02.009

    Repository Public URL： https://hdl.handle.net/2324/7172689

  • A novel strategy to facilitate uniform epithelial cell maturation using liquid–liquid interfaces

    Sonoi Rie, Kamihira Masamichi

    Scientific Reports   14 ( 1 )   12314   2024.5   （ eISSN:20452322 ）

    　More details

    Language：English  

    Epithelial tissue forms and maintains a critical barrier function in the body. A novel culture design aimed at promoting uniform maturation of epithelial cells using liquid materials is described. Culturing Madin–Darby canine kidney (MDCK) cells at the liquid–liquid interface yielded reduced migration and stimulated active cell growth. Similar to solid–liquid interfaces, cells cultured on a fibronectin-coated liquid–liquid interface exhibited active migration and growth, ultimately reaching a confluent state. These cells exhibited reduced stress fiber formation and adopted a cobblestone-like shape, which led to their even distribution in the culture vessel. To inhibit stress fiber formation and apoptosis, the exposure of cells on liquid–liquid interfaces to Y27632, a specific inhibitor of the Rho-associated protein kinase (ROCK), facilitated tight junction formation (frequency of ZO-2-positive cells, F_Z = 0.73). In Y27632-exposed cells on the liquid–liquid interface, the value obtained by subtracting the standard deviation of the ratio of nucleus densities in each region that compartmentalized a culture vessel from 1, denoted as H_<LN>, was 0.93 ± 0.01, indicated even cell distribution in the culture vessel at t = 72 h. The behavior of epithelial cells on liquid–liquid interfaces contributes to the promotion of their uniform maturation.

    CiNii Research

  • A novel strategy to facilitate uniform epithelial cell maturation using liquid-liquid interfaces

    Sonoi, R; Kamihira, M

    SCIENTIFIC REPORTS   14 ( 1 )   12314   2024.5   （ ISSN:2045-2322 ）

    　More details

    Language：English   Publisher：Scientific Reports  

    Epithelial tissue forms and maintains a critical barrier function in the body. A novel culture design aimed at promoting uniform maturation of epithelial cells using liquid materials is described. Culturing Madin–Darby canine kidney (MDCK) cells at the liquid–liquid interface yielded reduced migration and stimulated active cell growth. Similar to solid–liquid interfaces, cells cultured on a fibronectin-coated liquid–liquid interface exhibited active migration and growth, ultimately reaching a confluent state. These cells exhibited reduced stress fiber formation and adopted a cobblestone-like shape, which led to their even distribution in the culture vessel. To inhibit stress fiber formation and apoptosis, the exposure of cells on liquid–liquid interfaces to Y27632, a specific inhibitor of the Rho-associated protein kinase (ROCK), facilitated tight junction formation (frequency of ZO-2-positive cells, FZ = 0.73). In Y27632-exposed cells on the liquid–liquid interface, the value obtained by subtracting the standard deviation of the ratio of nucleus densities in each region that compartmentalized a culture vessel from 1, denoted as HLN, was 0.93 ± 0.01, indicated even cell distribution in the culture vessel at t = 72 h. The behavior of epithelial cells on liquid–liquid interfaces contributes to the promotion of their uniform maturation.

    DOI： 10.1038/s41598-024-63115-7

    Web of Science

    Scopus

    PubMed

▼display all

Presentations

  • A novel strategy to facilitate uniform epithelial cell maturation using liquid–liquid interfaces

    Rie Sonoi; Masamichi Kamihira

    2024.10 

    　More details

    Event date： 2024.10

    Language：Japanese  

    Country：Japan  

  • 光干渉断層撮影法による薬物性胆汁鬱滞を誘導したHepaRG凝集塊に対する非侵襲的評価

    園井理惠，山川健，中谷徳幸，小久保正彦，萩原義久

    第20回日本再生医療学会  2021.3 

    　More details

    Language：Japanese  

    Venue：神戸   Country：Japan  

  • 細胞遊走測定によるヒト網膜色素上皮細胞の成熟過程の解析

    園井理惠，金美海，紀ノ岡正博

    化学工学会第78回春季大会  2013.3 

    　More details

    Language：Japanese  

    Venue：大阪   Country：Japan  

  • 細胞加工施設における紙ベース原本のデータインテグリティ体制の整備

    園井理惠，福永素子，鍛谷香織，百田禎郎，田中芳浩，中西洋一，岡﨑利彦

    第17回日本再生医療学会  2018.3 

    　More details

    Language：Japanese  

    Venue：横浜   Country：Japan  

  • 細胞加工施設の管理者向け法規（製造・品質保証関連）の理解度評価クラウド型システムの開発

    園井理惠，福永素子，鍛谷香織，百田禎郎，田中芳浩，中西洋一，岡﨑利彦

    第17回日本再生医療学会  2018.3 

    　More details

    Language：Japanese  

    Venue：横浜   Country：Japan  

  • 細胞調製施設内における昆虫モニタリングにより採取同定された昆虫相から見る衛生マネジメント

    園井理惠，福永素子，鍛谷香織，百田禎郎，田中芳浩，中西洋一，岡﨑利彦

    第17回日本再生医療学会  2018.3 

    　More details

    Language：Japanese  

    Venue：横浜   Country：Japan  

  • 薬物性胆汁鬱滞を誘導したHepaRG凝集塊の細胞増殖性と毛細胆管の再生

    園井理惠，萩原義久

    第20回日本再生医療学会  2021.3 

    　More details

    Language：Japanese  

    Venue：神戸   Country：Japan  

  • Analysis of maturation of human retinal pigment epithelial cells in confluent state through a measurement of cell migration International conference

    Sonoi, R., Kim, M.H., and Kino-oka, M.

    Aachen-Osaka Symposium  2012.12 

    　More details

    Language：English  

    Country：Germany  

  • コンフルエント状態における網膜色素上皮細胞の遊走性による細胞成熟度の解析

    園井理惠，備瀬竜馬，金美海，紀ノ岡正博

    化学工学会第44回秋季大会  2012.9 

    　More details

    Language：Japanese  

    Venue：仙台   Country：Japan  

  • 深層学習の動画像認識技術を用いた細胞機能評価システムの開発

    #増原 誠, #片岡 笙, @園井 理惠, @河邉 佳典, @上平 正道

    第75回日本生物工学会大会  2023.9 

    　More details

    Event date： 2023.9

    Language：Japanese  

    Venue：名古屋大学 東山キャンパス (名古屋市千種区不老町)   Country：Japan  

  • 「再生医療等の安全性の確保等に関する法律」に準じた記録書の整備

    田所美香，江副幸子，金井浩美，大河原弘達，寺尾尚子，蒲克郎，園井理惠，北島英樹，齋藤充弘，名井陽，竹原徹郎，西田幸二

    第15回日本再生医療学会  2016.3 

    　More details

    Language：Japanese  

    Venue：大阪   Country：Japan  

  • エンドトキシン測定簡便法システムの再生医療分野での応用の可能性

    大河原弘達，園井理惠，高須賀禎浩，高岡文，江副幸子，名井陽，竹原徹郎，西田幸二

    第15回日本再生医療学会  2016.3 

    　More details

    Language：Japanese  

    Venue：大阪   Country：Japan  

  • ヒト滑膜由来間葉系幹細胞バンクの運用と今後の展開

    岡本美奈，下村和範，前達雄，中田研，中村憲正，照井雅子，山本尚子，前田悟，阪上守人，丸山秩弘，寺谷明恵，園井理惠，江副幸子，竹原徹郎，西田幸二，吉川秀樹，名井陽

    ARO協議会  2016.8 

    　More details

    Language：Japanese  

    Venue：大阪   Country：Japan  

  • 日本薬局方第十七改正に対応したマイコプラズマ否定試験・核酸増幅法の比較検討

    大河原弘達，園井理惠，名井陽，江副幸子，竹原徹郎，西田幸二

    第16回日本再生医療学会  2017.3 

    　More details

    Language：Japanese  

    Venue：仙台   Country：Japan  

  • 大阪大学医学部附属病院細胞培養調製施設における新法下製造の年間評価

    田所美香，江副幸子，寺尾尚子，大河原弘達，園井理惠，斎藤充弘，名井陽，竹原徹郎，西田幸二

    第16回日本再生医療学会  2017.3 

    　More details

    Language：Japanese  

    Venue：仙台   Country：Japan  

  • 企業治験の製造を支援するための大阪大学医学部附属病院細胞培養調製施設（MTR CPC）の取組み

    寺尾尚子，江副幸子，田所美香，大河原弘達，園井理惠，斎藤充弘，名井陽，竹原徹郎，西田幸二

    第16回日本再生医療学会  2017.3 

    　More details

    Language：Japanese  

    Venue：仙台   Country：Japan  

  • 血管新生における内皮細胞の集団運動を制御する分子メカニズム

    福原茂朋，若山勇紀，藤原正和，園井理惠，望月直樹

    第17回日本再生医療学会  2018.3 

    　More details

    Language：Japanese  

    Venue：香川   Country：Japan  

  • Validation of analytical procedures using BacT/ALERT 3D Microbial Detection System International conference

    Okazaki T., Kajitani K., Fukunaga M., Sonoi R., and Tanaka Y., and Nakanishi Y.

    TERMIS World Congress  2018.9 

    　More details

    Language：English  

    Country：Japan  

  • Development of Comprehension Evaluation System for Laws and Regulations of Regenerative Medicine International conference

    Okazaki T., Sonoi R., Fukunaga M., Kajitani K., Tanaka Y., and Nakanishi Y.

    TERMIS World Congress  2018.9 

    　More details

    Language：English  

    Country：Japan  

  • Quantitative evaluation of human retinal epithelial cells in confluent states International conference

    Sonoi, R., Kim, M.H., and Kino-oka, M.

    The 11th Global COE international symposium  2011.12 

    　More details

    Language：English  

    Country：Japan  

  • Cell aggregation and subsequent phenotype expression of multi-passaged human chondrocytes in rotational culture International conference

    Sonoi, R., Yamamoto, S., Hayashi, S., Furusaki, S., and Shioya, S.

    Asia Pacific Biochemical Engineering Conference  2009.11 

    　More details

    Language：English  

    Country：Japan  

  • Growth Characteristics and Subsequent Chondrogenesis of Subcultured Human Chondrocyte in Cell Aggregate Culture under Low Oxygen Tension International conference

    Sonoi, R., Yamamoto, S., Hayashi, S., and Shioya, S.

    Asian Congress on Biotechnology 2011  2011.5 

    　More details

    Language：English  

    Country：China  

  • 深層学習の動画像認識技術を用いた細胞機能評価システムの開発

    増原 誠, 片岡 笙, 園井 理惠, 河邉 佳典, 上平 正道

    日本生物工学会大会講演要旨集  2023.8  (公社)日本生物工学会

    　More details

    Language：Japanese  

▼display all

Professional Memberships

  • The Japanese Society for Biomaterials

  • Parenteral Drug Association

  • The japanese society for regenerative medicine

  • The society for biotechnology

Research Projects

  • 材料科学と遡及的解析を基にした高品質上皮組織工学の実現

    Grant number：23K17216  2023 - 2024

    日本学術振興会  科学研究費助成事業  若手研究

    園井 理惠

    　 More details

    Authorship：Principal investigator  Grant type：Scientific research funding

    生命機能維持には均一な上皮極性形成が望ましく，再生医療等製品の非侵襲的な評価や制御ができれば，貴重な細胞を損失することなく，高品質な製品提供につながる．本研究では，上皮細胞組織を効率的に形成する方法を探索し，高品質な再生医療等製品の生産に寄与することを目指す．その中で，上皮機能の発現に欠かせない上下軸形成に着目し，デザインされた基底側からの力学刺激を通じて高速且つ均一に上下軸形性を誘導する方法を確立する．

    CiNii Research