2024/09/29 更新

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写真a

モリ タケシ
森 健
MORI TAKESHI
所属
工学研究院 応用化学部門 准教授
未来化学創造センター (併任)
システム生命科学府 システム生命科学専攻(併任)
工学部 応用化学科(併任)
工学府 応用化学専攻(併任)
職名
准教授
プロフィール
生命現象を化学の視点(分子集合体、化学反応)で捉えて、医療に応用するための技術を開発している。具体的には、免疫を制御する分子、効率的な腫瘍への薬物送達法、蛍光法に基づく高精度な診断技術などである。
外部リンク

学位

  • 博士(工学)

経歴

  • 徳島大学 2001年4月〜2005年10月   

研究テーマ・研究キーワード

  • 研究テーマ: 腸内細菌叢を保護する抗生物質の経口投与法

    研究キーワード: 腸内細菌叢、抗生物質、経口投与

    研究期間: 2016年10月 - 2018年6月

  • 研究テーマ: ビタミン内包脂質粒子による炎症性疾患治療

    研究キーワード: ビタミン、脂質、炎症性腸疾患、喘息

    研究期間: 2015年10月 - 2018年6月

  • 研究テーマ: 膜貫通タンパク質の最小ミメティクス

    研究キーワード: 膜貫通タンパク質

    研究期間: 2015年4月

  • 研究テーマ: 内在性抗体を活用するがん治療のための中分子化合物の開発

    研究キーワード: 抗体医薬、がん

    研究期間: 2014年4月 - 2020年5月

  • 研究テーマ: 一酸化窒素を活用するナノメディスンの高効率ながんへの送達

    研究キーワード: 一酸化窒素, ナノメディスン, がん

    研究期間: 2014年4月 - 2020年5月

  • 研究テーマ: MHCを標的とする薬物送達システム

    研究キーワード: MHC, 薬物送達

    研究期間: 2013年4月 - 2018年6月

  • 研究テーマ: 免疫細胞の表面修飾による機能化

    研究キーワード: 免疫細胞 循環がん細胞

    研究期間: 2012年4月 - 2013年3月

  • 研究テーマ: 細胞表面ソフトエンジニアリングに基づく高効率エンドサイトーシス法

    研究キーワード: 細胞、エンドサイト―シス、ポリマー

    研究期間: 2011年4月 - 2013年3月

  • 研究テーマ: 共レセプター法

    研究キーワード: 細胞表面修飾

    研究期間: 2011年4月 - 2012年5月

  • 研究テーマ: 共エンドサイトーシス法

    研究キーワード: 細胞表面修飾

    研究期間: 2011年4月 - 2012年5月

  • 研究テーマ: 膜透過ポリマーの開発

    研究キーワード: 膜透過、ポリマー

    研究期間: 2011年4月 - 2011年5月

  • 研究テーマ: 次世代たんぱく質デリバリー素材:たんぱく質を温和に保持し放出するナノマシン

    研究キーワード: デリバリー、ナノマシン

    研究期間: 2010年9月 - 2011年5月

  • 研究テーマ: 生体環境応答性ペプチドのスクリーニングとバイオマテリアルへの応用

    研究キーワード: バイオマテリアル、環境応答性ペプチド

    研究期間: 2010年4月 - 2012年3月

  • 研究テーマ: 細胞内シグナル応答性遺伝子治療用高分子キャリアの開発

    研究キーワード: 遺伝子治療、シグナル伝達

    研究期間: 2006年4月

  • 研究テーマ: 高感度ペプチドアレイの開発と診断・創薬への応用

    研究キーワード: ペプチドアレイ、診断、創薬

    研究期間: 2006年4月

  • 研究テーマ: ヒステリシスを持つ温度応答性高分子の開発とバイオメディカル応用

    研究キーワード: バイオメディカルポリマー、DDS

    研究期間: 2005年4月

受賞

  • 高分子学会奨励賞

    2010年5月   高分子学会   合成高分子を用いたタンパク質相転移現象解明へのアプローチ

  • 九州分析化学奨励賞

    1999年7月   分析化学会   遺伝子DNAの精密分離

論文

  • Safe and efficient oral allergy immunotherapy using one-pot-prepared mannan-coated allergen nanoparticles 査読 国際誌

    S. Li, H. Toriumi, D. Takahashi, T. Kamasaki, Y. Fujioka, S. Nagatoishi, J. Li, Y. Liu, T. Hosokawa, K. Tsumoto, Y. Ohba, Y. Katayama, D. Murakami, K. Hase, T. Mori

    Biomaterials   303   122381   2023年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    リポジトリ公開URL: https://hdl.handle.net/2324/7177976

  • A human cell orthogonal enzyme β-D-galacturonidase for sensitive detection of antigen proteins 招待 査読 国際誌

    C. Tateishi, A. Koga, A. Matsuura, R. Kaneko, K. Tanito, T. Nii, Akihiro Kishimura, T. Mori, Y. Katayama

    Analyst   148   2237 - 2244   2023年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    リポジトリ公開URL: https://hdl.handle.net/2324/7178528

  • Quiescent B cells acquire sensitivity to cell cycle arresting agents by B cell receptor stimulation 招待 査読 国際誌

    T. Hosokawa, S. Tanaka, T. Mori, Y. Baba, and Y. Katayama

    Biol. Pharm. Bull.   45   847 - 850   2022年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Specific adsorption of a β-lactam antibiotic in vivo by an anion-exchange resin for protection of the intestinal microbiota 招待 査読 国際誌

    S. Li, K. Yakabe, K. Zai, Y. Liu, A. Kishimura, K. Hase, Y.-G. Kim, T. Mori, Y. Katayama

    Biomater. Sci.   9   7219 - 7227   2021年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Fc-binding antibody-recruiting molecules targeting prostate-specific membrane antigen: defucosylation of antibody for efficacy improvement, 招待 査読 国際誌

    K. Sasaki, M. Harada, T. Yoshikawa, H. Tagawa, Y. Harada, Y. Yonemitsu, T. Ryujin, A. Kishimura, T. Mori, Y. Katayama

    ChemBioChem   22   496 - 500   2021年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Polyvinyl butyrate nanoparticles as butyrate donors for colitis treatment 招待 査読 国際誌

    Y. Mu, Y. Kinashi, J. Li, T. Yoshikawa, A. Kishimura, M. Tanaka, T. Matsui, T. Mori, K. Hase, Y. Katayama

    ACS Applied Bio Mater.   4   2335   2021年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Ligand design for specific MHC class I molecules on the cell surface 査読 国際誌

    X. Sun, R. Tokunaga, Y. Nagai, R. Miyahara, A. Kishimura, S. Kawakami, Y. Katayama, T. Mori

    Biochemistry   59   4646   2020年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Protection of gut microbiome from antibiotics: development of a vancomycin-specific adsorbent with high adsorption capacity 査読 国際誌

    K. Yuzuriha, K. Yakabe, H. Nagai, S. Li, T. Zendo, K. Zai, A. Kishimura, K. Hase, Y-G Kim, T. Mori, Y. Katayama

    Biosci. Microbio. Food Health   39   128 - 136   2020年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    腸内細菌叢を破壊することが知られる抗生物質(バンコマイシン)を腸間で特異的に吸着する粒子を開発し、実際に、腸内細菌を保護することを示した。また細菌叢の破壊に由来する感染症からマウスを保護することができた。

  • Synthesis of peptide conjugates with vitamins for induction of antigen-specific immunotolerance 査読 国際誌

    K. Yuzuriha, A. Yoshida, S. Li, A. Kishimura, T. Mori, Y. Katayama

    J. Peptide Sci.   26   e3275   2020年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Β-galactosidase-catalyzed fluorescent reporter labeling of living cells for sensitive detection of cell surface antigens 査読 国際誌

    K. Noguchi, T. Shimomura, Y. Ohuchi, M. Ishiyama, M. Shiga, T. Mori, Y. Katayama, Y. Ueno

    Bioconjugate Chem.   31   1740 - 1744   2020年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    beta-ガラクトシダーゼを酵素として、一細胞の抗原を高感度に増感検出する方法(蛍光がターンオンし、細胞に共有結合して脱離しない)の開発に初めて成功した。

  • Blood retention and antigenicity of polycarboxybetaine-modified liposomes 招待 査読 国際誌

    T. Ryujin, T. Shimizu, R. Miyahara, D. Asai, R. Shimazui, T. Yoshikawa, A. Kishimura, T. Mori, T. Ishida, Y. Katayama

    Int. J. Pharm.   586   119521   2020年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Fc-binding antibody-recruiting molecules exploit endogenous antibodies for anti-tumor immune responses 招待 査読 国際誌

    K. Sasaki, M. Harada, Y. Miyashita, H. Tagawa, A. Kishimura, T. Mori, Y. Katayama,

    Chem. Sci.   11   3208 - 3214   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    抗体医薬を低分子化合物で置き換えることを企図し、がん細胞と内在性の抗体を架橋する低分子を開発した。この分子は、in vitro, in vivoで抗がん活性を示した。

  • Modification of nitric oxide donors onto a monoclonal antibody boosts accumulation in solid tumors 査読 国際誌

    T. Yoshikawa, K. Q. Phan, H. Tagawa, K. Sasaki, H. Feng, A. Kishimura, T. Mori, Y. Katayama

    Int. J. Pharm.   583   119352   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Induction of ADCC by folic acid-mAb conjugate prepared by tryptophan-selective reaction toward folate-receptor-positive cancer cells 招待 査読 国際誌

    H. Tagawa, K. Maruyama, K. Sasaki, N. Konoue, A. Kishimura, M. Kanai, T. Mori, K. Oisaki, Y. Katayama

    RSC Adv.   10   16727 - 16731   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Fluorescence Signal Amplification by Using β-Galactosidase for Flow Cytometry; Advantages of an Endogenous Activity-free Enzyme, 査読 国際誌

    T. Nobori, M. Kawamura, R. Yoshida, T. Joichi, K. Kamino, A. Kishimura, E. Baba, T. Mori, Y. Katayama,

    Anal. Chem.   92   3069 - 3076   2020年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Therapeutic effect of vitamin D3-containing nanostructured lipid carriers on inflammatory bowel disease 招待 査読 国際誌

    K. Zai, M. Hirota, T. Yamada, N. Ishihara, T. Mori, A. Kishimura, K. Suzuki, K. Hase, Y. Katayama

    J. Controlled Release   286   94 - 102   2018年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • A Peptide Inhibitor of Antibody-Dependent Cell-Mediated Cytotoxicity against EGFR/Folate Receptor-α Double Positive Cells 招待 査読 国際誌

    K. Sasaki, Y. Miyashita, D. Asai, D. Funamoto, K. Sato, Y. Yamaguchi, Y. Mishima, T. Iino, S. Takaishi, J. Nagano, A. Kishimura, T. Mori, Y. Katayama

    Med. Chem. Commun.   9   783 - 788   2018年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Utilization of a PNA-peptide Conjugate to Induce a Cancer Protease-Responsive RNAi Effect 査読 国際誌

    E. K. Lee, C. W. Kim, H. Kawanami, Akihiro Kishimura, T. Niidome, Takeshi Mori, Yoshiki Katayama

    RSC Adv.   5   85816   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    PNA-peptideコンジュゲートにより、腫瘍において選択的にRNAiを生じるシステムを開発した。腫瘍で高活性化しているcathepsin Bを標的にしたシステムであるが、一般性が高く、望みのプロテアーゼに対して設計可能である。

  • Antibody Internalization into Living Cell via Crosslinker-mediated Endocytosis 査読 国際誌

    D. Funamoto, D. Asai, K. Sato, Y. Yamaguchi, C. W. Kim, H. Sato, E. Nakhaei, S. Matsumoto, T. Yoshikawa, K. Sasaki, T. Yamamoto, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

    Chem. Lett.   44   468   2015年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Rapid and serum-insensitive endocytotic delivery of proteins using biotinylated polymers attached via multivalent hydrophobic anchors 査読 国際誌

    K Tobinaga, C Li, M Takeo, T Niidome, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

    J. Controlled Release   177   27   2014年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Short Peptide Motifs for Long-Lasting Anchoring to the Cell Surface 査読

    M. Matsuda, W. Hatanaka, M. Takeo, C. W. Kim, T. Niidome, T. Yamamoto, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

    Bioconjugate Chem.   25   2134   2014年8月

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    記述言語:英語  

  • Histidinylated poly-L-lysine-based vectors for cancer-specific gene expression via enhancing the endosomal escape 査読 国際誌

    GX Zhao, H Tanaka, CW Kim, K Li, D Funamoto, T Nobori, Y Nakamura, T Niidome, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

    J. Biomater. Sci. Polym. Ed.   25   519   2014年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • CW/pulsed NIR irradiation of gold nanorods: Effect on transdermal protein delivery mediated by photothermal ablation 査読 国際誌

    Takeshi Mori, H. Tang, H. Kobayashi, Y. Niidome, Yoshiki Katayama, T. Niidome

    J. Controlled Release   171   178 - 183   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Stabilization of Cancer-specific Gene Carrier via Hydrophobic Interaction for a Clear-cut Response to Cancer Signaling 査読 国際誌

    C. W. Kim, R. Toita, J.-H. Kang, K. Li, E. K. Lee, G. X. Zhao, D. Funamoto, T. Nobori, Takeshi Mori, T. Niidome, Y. Nakamura, Yoshiki Katayama

    J. Controlled Release   170   469 - 476   2013年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Serum protein kinase Cα as a diagnostic biomarker of cancers 査読 国際誌

    J.-H. Kang, Takeshi Mori, H. Kitazaki, T. Niidome, Y. Nakanishi, K. Takayama, Yoshiki Katayama

    Cancer Biomarker   13   99 - 103   2013年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • A peptide microarray fabricated on a non-fouling phosphatidylcholine-polymer-coated surface for a high-fidelity analysis of a cellular kinome 査読 国際誌

    H. Ikeda, J. Kamimoto, T. Yamamoto, A. Hata, Y. Otsubo, T. Niidome, M. Fukushima, Takeshi Mori, Yoshiki Katayama

    Current Med. Chem.   20   4419 - 25   2013年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Branched polyethylenimine-based PKCα-responsive gene carriers 査読 国際誌

    Y. Nakamura, C. W. Kim, A. Tsuchiya, S. Kushio, K. Li, T. Nobori, E. K. Lee, G. Xi Zhao, D. Funamoto, T. Niidome, Takeshi Mori, Yoshiki Katayama

    J. Biomater. Sci.-Polym. Ed.   24   1858 - 1868   2013年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Design of polymeric carriers for cancer-specific gene targeting: Utilization of abnormal protein kinase Cα activation in cancer cells 国際誌

    J.-H. Kang, D. Asai, J.-H. Kim, T. Mori, R. Toita, T. Tomiyama, Y. Asami, J. Oishi, Y. T. Sato, T. Niidome, B. Jun, H. Nakashima, Y. Katayama

    J. Am. Chem. Soc   2009年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Syndiotactic poly(N-n-propylacrylamide) shows highly cooperative phase transition

    T. Mori, T. Hirano, A. Maruyama, Y. Katayama, T. Niidome, Y. Bnado, K. Ute, S. Takaku, Y. Maeda

    Langmuir   25   2009年10月

  • Monitoring protein kinase activity in cell lysates using a high density peptide microarray 国際誌

    X. Han, G. Yamanouchi, T. Mori, J.-H. Kang, T. Niidome, Y. Katayama

    J. Biomol. Screen.   2009年7月

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    掲載種別:研究論文(学術雑誌)  

  • Syndiotactic poly(N-n-propylacrylamide) shows highly cooperative phase transition 国際誌

    T. Mori*, T. Hirano, A. Maruyama, Y. Katayama, T. Niidome, Y. Bnado, K. Ute, S. Takaku, Y. Maeda

    Langmuir   2009年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Measurement of Homogeneous Kinase Activity for Cell Lysates Based on Aggregation of Gold Nanoparticles 査読 国際誌

    J. Oishi, Y. Asami, T. Mori*, J.-H. Kang, M. Tanabe, T. Niidome, Y. Katayama

    ChemBioChem   2007年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Measurement of homogeneous kinase activityfor cell lysates based on the aggregation of gold nanoparticles 国際誌

    Measurement of homogeneous kinase activityfor cell lysates based on the aggregation of gold nanoparticles

    ChemBioChem   2007年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Impact of hydrophobic modification on biocompatibility of Alaska pollock gelatin microparticles

    Yee, YC; Mori, T; Ito, S; Taguchi, T; Katayama, Y

    ANALYTICAL SCIENCES   2024年8月   ISSN:0910-6340 eISSN:1348-2246

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    記述言語:英語   出版者・発行元:Analytical Sciences  

    This study investigates the impact of hydrophobic modification on the immunogenicity, cytotoxicity, and inflammatory response of Alaska pollock gelatin (ApGltn) microparticles (MPs). Gelatin, known for its inherent biocompatibility, was modified with decyl group (C10) to explore potential alterations in its interaction with the immune system. Immunogenicity was evaluated through the measurement of material-specific IgM and IgG responses, indicating no significant increase post-modification. Cytotoxicity against Caco-2 cell lines and NF-κB-mediated LPS-induced inflammation were also assessed, revealing no exacerbation by the modified MPs. Furthermore, C10 modification with different types of linkage such as secondary amine and amide structure did not influence immune reactivity. These findings suggest that C10 modification maintains the non-immunogenicity and biocompatibility of gelatin MPs, supporting their potential use in biomedical applications. Graphical abstract: (Figure presented.).

    DOI: 10.1007/s44211-024-00643-2

    Web of Science

    Scopus

    PubMed

  • Establishment of an in vitro evaluation method for immunomodulatory functions of yeast strains

    Yee, YC; Nakamura, A; Okada, Y; Mori, T; Katayama, Y

    ANALYTICAL SCIENCES   2024年8月   ISSN:0910-6340 eISSN:1348-2246

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    記述言語:英語   出版者・発行元:Analytical Sciences  

    Saccharomyces cerevisiae, a widely studied yeast known for its industrial applications, is increasingly recognized for its potential in immunomodulation. This study aimed to systematically analyze and compare the immune-modulating properties of various S. cerevisiae strains under controlled experimental conditions. Three essential signals crucial for immune response activation were evaluated to elucidate the immunological responses elicited by these strains, i.e., dendritic cells (DC) cytokine secretion profiles, maturation status, and T cell polarization. Analysis of DC cytokine secretion profiles and maturation status revealed that all tested yeast strains induced DC activation, characterized by significant IL-6 secretion and modest IL-10 induction, as well as upregulation of MHC II molecules. Additionally, strain-specific effects were observed, particularly, strain AJM109 and Y1383 uniquely enhanced CD86 and PD-L1 expression, respectively, suggesting differential impacts on DC co-stimulatory signaling. Furthermore, strain Y1383 showed a unique capacity to support Treg-mediated immune suppression, demonstrating its potential in immune tolerance induction. These findings underscore the complexity of S. cerevisiae-based immune modulation and emphasize the importance of standardized evaluation methods to distinguish their specific immunological effects. Graphical abstract: (Figure presented.)

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  • In vitro evaluation of novel SN-38 prodrug activated by α-rhamnosidase of exogenous enzyme

    Nii, T; Hijii, S; Kaneko, R; Tanito, K; Yamanaka, K; Kishimura, A; Mori, T; Katayama, Y

    ANALYTICAL SCIENCES   40 ( 8 )   1529 - 1535   2024年8月   ISSN:0910-6340 eISSN:1348-2246

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Analytical Sciences  

    This study introduces the α-rhamnose (Rham)-conjugated prodrug of SN-38 (Rham-SN-38) as a promising alternative to irinotecan. α-rhamnosidase, responsible for SN-38 release from Rham-SN-38, does not express in human cells, minimizing individual variability and side effects. The injection of the α-rhamnosidase into the tumor tissues makes it possible, for the first time, to activate the Rham-SN-38. Furthermore, α-rhamnosidase demonstrates significantly higher activity than carboxylesterase, the specific enzyme activating irinotecan. SN-38 release mediated by α-rhamnosidase completes within 2 h, with a kcat/Km value approximately 5.0 × 104-fold higher than that of irinotecan. The 50% inhibition concentration (IC50) of Rham-SN-38 against three types of cancer cells and one normal cell exceeds 4.5 × 103 nM. The addition of α-rhamnosidase significantly increases cytotoxicity, with IC50 comparable to free SN-38. The QIC50, an index reflecting the difference in cytotoxicity with and without α-rhamnosidase, exceeds approximately 1.0 × 102-fold. Rham-SN-38, synthesized in this study, demonstrates significant potential as a prodrug for cancer therapy. Graphical abstract: (Figure presented.)

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  • Accurate evaluation of drug effect on the LDH activity of live cells: dual measurement of live cell number by fluorescent staining of nucleus and LDH activity by formazan

    Kaneko, R; Hirakawa, R; Hijii, S; Mori, T; Katayama, Y

    ANALYTICAL SCIENCES   2024年7月   ISSN:0910-6340 eISSN:1348-2246

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    記述言語:英語   出版者・発行元:Analytical Sciences  

    Effect of drugs on the intracellular activity of lactate dehydrogenase (LDH) has been measured by using water-soluble tetrazolium (WST). Because the assay is usually conducted in the presence of dead cells, net activity of live cells is not evaluated. Here, we reported the assay of the net intracellular LDH activity of live cells by counting the live cells using fluorescent staining of nucleus. By using a deep red fluorescent dye, dual measurements of fluorescence signal of nucleus and absorbance of WST could be conducted with transparent 96-well-plates. We found that conventional assay in the presence of dead cells overestimate the effect of drugs on the LDH activity. Graphical Abstract: (Figure presented.)

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  • Effective design of PEGylated polyion complex (PIC) nanoparticles for enhancing PIC internalisation in cells utilising block copolymer combinations with mismatched ionic chain lengths

    Aulia, F; Matsuba, H; Adachi, S; Yamada, T; Nakase, I; Nii, T; Mori, T; Katayama, Y; Kishimura, A

    JOURNAL OF MATERIALS CHEMISTRY B   12 ( 7 )   1826 - 1836   2024年2月   ISSN:2050-750X eISSN:2050-7518

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Journal of Materials Chemistry B  

    In nanomedicine, PEGylation of nanomaterials poses a dilemma since it inhibits their interaction with target cells and enables their retention in target tissues despite its biocompatibility and nonspecific internalisation suppression. PEGylated polypeptide-based polyion complexes (PICs) are fabricated via the self-assembly of PEGylated aniomers and homocatiomers based on electrostatic interactions. We propose that various parameters like block copolymer design and PIC domain characteristics can enhance the cell-PEGylated PIC interactions. Remarkably, the properties of the PIC domain were tuned by the matched/mismatched ionomer chain lengths, PIC domain crosslinking degree, chemical modification of cationic species after crosslinking, PIC morphologies (vesicles/micelles) and polyethylene glycol (PEG) chain lengths. Cellular internalisation of the prepared PICs was evaluated using HeLa cells. Consequently, mismatched ionomer chain lengths and vesicle morphology enhanced cell-PIC interactions, and the states of ion pairing, particularly cationic residues, affected the internalisation behaviours of PICs via acetylation or guanidinylation of amino groups on catiomers. This treatment attenuated the cell-PIC interactions, possibly because of reduced interaction of PICs with negatively charged species on the cell-surface, glycosaminoglycans. Moreover, morphology and PEG length were correlated with PIC internalisation, in which PICs with longer and denser PEG were internalised less effectively. Cell line dependency was tested using RAW 264.7 macrophage cells; PIC recognition could be maintained after capping amino groups on catiomers, indicating that the remaining anionic groups were still effectively recognised by the scavenger receptors of macrophages. Our strategy for tuning the physicochemical properties of the PEGylated PIC nanocarriers is promising for overcoming the PEG issue.

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  • Activated tyrosine kinase c-Src-responsive artificial gene carrier: <i>in vitro</i> and <i>in vivo</i> evaluation

    Terada, K; Asai, D; Kang, JH; Mori, T; Katayama, Y

    CHEMISTRYSELECT   9 ( 1 )   2024年1月   ISSN:2365-6549 eISSN:2365-6549

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:ChemistrySelect  

    The proto-oncogene c-Src is a protein-tyrosine kinase that is associated with increased risk of acute and chronic kidney diseases, cardiovascular diseases, neurological diseases, and cancers. The purpose of this study was to develop a gene delivery carrier responding to activated c-Src and to evaluate its efficacy in vitro and in vivo. The polymeric gene carrier consists of a neutral main chain bearing a peptide substrate of c-Src or negative control peptide. Complexes of the polymer containing c-Src-targeting peptides and DNA were phosphorylated in the presence of c-Src, resulting in release of the DNA. Green fluorescent protein (GFP) expression was observed after microinjection of complexes of polymer containing c-Src-targeting peptides and GFP-encoding DNA into A431 cells at the nitrogen/phosphate (N/P) ratio of 1.0. GFP was not expressed in cells microinjected with negative control polymer/DNA complex. Direct injection of complexes of polymer containing c-Src-targeting peptides and luciferase-encoding DNA at N/P ratios of 1.0 and 2.0 into tumor-bearing mice resulted in luciferase expression in A431 tumors but not in normal skin tissues. No luciferase expression was observed in A431 tumors or skin tissues after injection of control polymer/DNA complex. These results indicate that our gene delivery carrier enables activated c-Src-specific gene expression.

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  • First GC/MS identification of aqueous ammonia: utilization of ethenesulfonyl fluoride as a selective and rapid derivatization reagent of ammonia in aqueous media

    Shiraki, R; Wakigawa, K; Ogawa, S; Gohda, A; Mori, T; Katayama, Y

    ANALYTICAL METHODS   15 ( 40 )   5294 - 5299   2023年10月   ISSN:1759-9660 eISSN:1759-9679

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    記述言語:英語   出版者・発行元:Analytical Methods  

    Identification as well as quantification of ammonia are required in some analytical fields including forensic science. For this purpose, gas chromatography/mass spectrometry (GC/MS) analysis is one of the most suitable techniques. Although ammonia needs to be derivatized for GC/MS analysis, conventional derivatization reagents require anhydrous conditions because they are highly reactive with water. Here, we investigated ethenesulfonyl fluoride (ESF) as a selective reagent for ammonia derivatization in aqueous media to develop a rapid identification method for ammonia in aqueous media. The Michael addition reaction of ammonia with ESF rapidly produced a tri-ESF derivative suitable for GC/MS analysis. We optimized the derivatization reaction conditions and extraction solvent. With the optimized protocol, the detection limit for aqueous ammonia was 0.05 μg mL−1. The calibration curve showed good linearity (R2 = 0.9998) in the range of 0.10-100.0 μg mL−1, and the accuracy (% bias) and the precision (% relative standard deviation) for concentrations of 0.10, 0.25, 10.0, and 75.0 μg mL−1 were within ± 10% (intra- and inter-day). The proposed ESF-based method could quantify ammonia in samples containing interfering nucleophilic substances. This method was successfully applied to ammonia-containing commercial products.

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  • Near infrared photoluminescent detection of serum albumin based on selective recognition with a long-chain fatty acid tethered on locally functionalized single-walled carbon nanotubes 査読 国際誌

    Y. Niidome, K. Nakamura, S. Qi, S. Ito, B. Yu, Y. Nagai, N. Tanaka, T. Mori, Y. Katayama, T. Fujigaya, T. Shiraki,

    Carbon   2023年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • First GC/MS identification of aqueous ammonia: utilization of ethenesulfonyl fluoride as a selective and rapid derivatization reagent of ammonia in aqueous media 招待 査読 国際誌

    R. Shiraki, K. Wakigawa, S. Ogawa, A. Gohda, T. Mori, Y. Katayama

    Anal. Method.   15   5294 - 5299   2023年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Engineered macrophages acting as a trigger to induce inflammation only in tumor tissues

    Tanito K., Nii T., Yokoyama Y., Oishi H., Shibata M., Hijii S., Kaneko R., Tateishi C., Ito S., Kishimura A., Mori T., Katayama Y.

    Journal of Controlled Release   361   885 - 895   2023年9月   ISSN:01683659

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    出版者・発行元:Journal of Controlled Release  

    Herein, we report engineered macrophages, termed “MacTrigger,” acting as a trigger to induce an inflammatory environment only in tumor tissues. This led to intensive anti-tumor effects based on the removal potential of foreign substances. The strength of this study is the utilization of two unique functions of macrophages: (1) their ability to migrate to tumor tissues and (2) polarization into the anti-inflammatory M2 phenotype in the presence of tumor tissues. The MacTrigger accelerated the release of inflammatory cytokines, tumor necrosis factor-alpha (TNF-α), when it was polarized to the M2 phenotype. When the MacTrigger was administered to tumor-bearing mice, tumor growth was significantly inhibited compared with the non-treatment group, the un-transfected macrophages group, and the group with engineered macrophages capable of randomly releasing TNF-α. Additionally, the ratio of the M1 phenotype to the M2 phenotype in tumor tissues was >1 only in the MacTrigger group. Moreover, the ratios of natural killer cells and CD8+T cells in tumor tissues were increased compared with other groups. These results indicate that MacTrigger can induce inflammation in tumor tissues, leading to effective anti-tumor effects. In normal tissues, especially the liver, notable side effects were not observed. This is because, in the liver, the MacTrigger was not polarized to the M2 phenotype and could not induce inflammation. These results suggest that the MacTrigger is a “trigger” that can induce inflammation only in tumor tissues, then allowing the body to attack tumor tissues through the innate immunity system.

    DOI: 10.1016/j.jconrel.2023.04.010

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  • Engineered macrophages acting as a trigger to induce inflammation only in tumor tissues

    Tanito, K; Nii, T; Yokoyama, Y; Oishi, H; Shibata, M; Hijii, S; Kaneko, R; Tateishi, C; Ito, S; Kishimura, A; Mori, T; Katayama, Y

    JOURNAL OF CONTROLLED RELEASE   361   885 - 895   2023年9月   ISSN:0168-3659 eISSN:1873-4995

  • Dynamic frustrated charge hotspots created by charge density modulation sequester globular proteins into complex coacervates

    Biplab, KC; Nii, T; Mori, T; Katayama, Y; Kishimura, A

    CHEMICAL SCIENCE   14 ( 24 )   6608 - 6620   2023年6月   ISSN:2041-6520 eISSN:2041-6539

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Chemical Science  

    This study presents a simple strategy for the sequestration of globular proteins as clients into synthetic polypeptide-based complex coacervates as a scaffold, thereby recapitulating the scaffold-client interaction found in biological condensates. Considering the low net charges of scaffold proteins participating in biological condensates, the linear charge density (σ) on the polyanion, polyethylene glycol-b-poly(aspartic acids), was reduced by introducing hydroxypropyl or butyl moieties as a charge-neutral pendant group. Complex coacervate prepared from the series of reduced-σ polyanions and the polycation, homo-poly-l-lysine, could act as a scaffold that sequestered various globular proteins with high encapsulation efficiency (>80%), which sometimes involved further agglomerations in the coacervates. The sequestration of proteins was basically driven by electrostatic interaction, and therefore depended on the ionic strength and charges of the proteins. However, based on the results of polymer partitioning in the coacervate in the presence or absence of proteins, charge ratios between cationic and anionic polymers were maintained at the charge ratio of unity. Therefore, the origin of the electrostatic interaction with proteins is considered to be dynamic frustrated charges in the complex coacervates created by non-neutralized charges on polymer chains. Furthermore, fluorescence recovery after photobleaching (FRAP) measurements showed that the interaction of side-chains and proteins changed the dynamic property of coacervates. It also suggested that the physical properties of the condensate are tunable before and after the sequestration of globular proteins. The present rational design approach of the scaffold-client interaction is helpful for basic life-science research and the applied frontier of artificial organelles.

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  • Development of novel tracers for sentinel node identification in cervical cancer 招待 査読 国際誌

    K. Kodama, C. Tateishi, T. Oda, L. Cui, K. Kuramoto, H. Yahata, K. Okugawa, S. Maenohara, H. Yagi, M. Yasunaga, I. Onoyama, K. Asanoma, T. Mori, Y. Katayama, K. Kato

    Cancer Sci.   114   4216 - 4224   2023年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    リポジトリ公開URL: https://hdl.handle.net/2324/7178520

  • Characterization of polypropyleneimine as an alternative transfection reagent

    Saeki, R; Kobayashi, S; Shimazui, R; Nii, T; Kishimura, A; Mori, T; Tanaka, M; Katayama, Y

    ANALYTICAL SCIENCES   39 ( 6 )   1015 - 1020   2023年6月   ISSN:0910-6340 eISSN:1348-2246

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Analytical Sciences  

    Polypropyleneimine (PPI) was examined as a transfection reagent comparing with most widely used polymer, polyethyleneimine (PEI). PPI had better responsiveness to the endosomal pH and showed more condensation ability of plasmid DNA than PEI. Although the cytotoxicity of PPI was somewhat higher than PEI, the transfection efficacy of PPI was comparable with PEI or higher than PEI in some cell line. Thus, PPI would be an alternative transfection reagent. Graphical abstract: [Figure not available: see fulltext.].

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  • Dynamic frustrated charge hotspots created by charge density modulation sequester globular proteins into complex coacervates

    新居 輝樹, 森 健, 片山 佳樹, 岸村 顕広

    Chemical Science   14 ( 24 )   6608 - 6620   2023年5月   ISSN:20416520 eISSN:20416539

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    記述言語:英語   出版者・発行元:Royal Society of Chemistry (RSC)  

    This study presents a simple strategy for the sequestration of globular proteins as clients into synthetic polypeptide-based complex coacervates as a scaffold, thereby recapitulating the scaffold-client interaction found in biological condensates. Considering the low net charges of scaffold proteins participating in biological condensates, the linear charge density (σ) on the polyanion, polyethylene glycol-b-poly(aspartic acids), was reduced by introducing hydroxypropyl or butyl moieties as a charge-neutral pendant group. Complex coacervate prepared from the series of reduced-σ polyanions and the polycation, homo-poly-L-lysine, could act as a scaffold that sequestered various globular proteins with high encapsulation efficiency (>80%), which sometimes involved further agglomerations in the coacervates. The sequestration of proteins was basically driven by electrostatic interaction, and therefore depended on the ionic strength and charges of the proteins. However, based on the results of polymer partitioning in the coacervate in the presence or absence of proteins, charge ratios between cationic and anionic polymers were maintained at the charge ratio of unity. Therefore, the origin of the electrostatic interaction with proteins is considered to be dynamic frustrated charges in the complex coacervates created by non-neutralized charges on polymer chains. Furthermore, fluorescence recovery after photobleaching (FRAP) measurements showed that the interaction of side-chains and proteins changed the dynamic property of coacervates. It also suggested that the physical properties of the condensate are tunable before and after the sequestration of globular proteins. The present rational design approach of the scaffold-client interaction is helpful for basic life-science research and the applied frontier of artificial organelles.

    CiNii Research

  • A human cell orthogonal enzyme β-D-galacturonidase for sensitive detection of antigen proteins

    Tateishi, C; Koga, A; Matsuura, A; Kaneko, R; Tanito, K; Nii, T; Kishimura, A; Mori, T; Katayama, Y

    ANALYST   148 ( 10 )   2237 - 2244   2023年5月   ISSN:0003-2654 eISSN:1364-5528

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Analyst  

    Enzymes are used to amplify signals for detection of antigen proteins in biological samples. However, the enzymes conventionally used for this purpose have limitations, such as the presence of the same (i.e., endogenous) activity in human cells and difficulty in simultaneous use of multiple enzymes because of differences in their required reaction conditions. In this report, we identify an enzyme that can overcome these problems: β-d-galacturonidase (GalUAase) from Eisenbergiella tayi. GalUAase activity was confirmed to be absent from human cells. The substrate of GalUAase, galacturonic acid, is highly hydrophilic because of its anionic carboxylate group; high substrate hydrophilicity is an ideal characteristic for the substrate of an enzyme used for detection because it decreases nonspecific adsorption to biological samples. We show that E. tayi GalUAase could be used in the detection of antigen proteins on live human cells with lower background signal than the conventionally used enzyme β-d-galactosidase. The combinatorial use of GalUAase with β-d-galactosidase enabled simultaneous detection of two antigens on live cells.

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  • Engineered macrophages acting as a trigger to induce inflammation only in tumor tissues.

    Tanito K, Nii T, Yokoyama Y, Oishi H, Shibata M, Hijii S, Kaneko R, Tateishi C, Ito S, Kishimura A, Mori T, Katayama Y

    Journal of controlled release : official journal of the Controlled Release Society   2023年4月   ISSN:0168-3659

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    記述言語:英語  

    DOI: 10.1016/j.jconrel.2023.04.010

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  • Engineered macrophages acting as a trigger to induce inflammation only in tumor tissues based on arginase 1-responsive TNF-α accelerated release.

    Tanito K, Nii T, Yokoyama Y, Oishi H, Shibata M, Hijii S, Kaneko R, Tateishi C, Ito S, Kishimura A, Mori T, Katayama Y

    Journal of controlled release : official journal of the Controlled Release Society   2023年4月   ISSN:0168-3659

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  • Dynamic frustrated charge hotspots created by charge density modulation sequester globular proteins into complex coacervates, 招待 査読 国際誌

    A. Kishimura, K.C. Biplab, T. Nii, T. Mori, Y. Katayama

    Chem. Sci.   14   6608 - 6620   2023年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    リポジトリ公開URL: https://hdl.handle.net/2324/7173543

  • Engineered macrophages acting as a trigger to induce inflammation only in tumor tissues based on arginase 1-responsive TNF-α accelerated release 招待 査読 国際誌

    K. Tanito, T. Nii, Y. Yokoyama, H. Oishi, M. Shibata, S. Hijii, R. Kaneko, C. Tateishi, S. Ito, A. Kishimura, T. Mori, Y. Katayama

    J. Controlled Release   361   885 - 895   2023年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Facile preparation of hexagonal nanosheets via polyion complex formation from α-helical polypeptides and polyphosphate-based molecules 査読 国際誌

    A. Ahmad, T. Maruyama, T. Nii, T. Mori, Y. Katayama, A. Kishimura

    Chem. Commun.   59   1657 - 1660   2023年4月

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  • One-pot preparation of mannan-coated antigen nanoparticles using human serum albumin as a matrix for tolerance induction 招待 査読 国際誌

    S. Li, D. Murakami, S. Nagatoishi, K. Tsumoto, Y. Katayama, T. Mori

    J. Coll. Interf. Sci.   649   955 - 965   2023年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    リポジトリ公開URL: https://hdl.handle.net/2324/7178526

  • Engineered macrophages acting as a trigger to induce inflammation only in tumor tissues

    Kenta Tanito, Teruki Nii, Yuta Yokoyama, Haruka Oishi, Mayuka Shibata, Shoichi Hijii, Ryosuke Kaneko, Chuya Tateishi, Shoko Ito, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

    Journal of Controlled Release   2023年4月   ISSN:0168-3659

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

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  • Facile preparation of hexagonal nanosheets <i>via</i> polyion complex formation from α-helical polypeptides and polyphosphate-based molecules

    Ahmad, A; Maruyama, T; Nii, T; Mori, T; Katayama, Y; Kishimura, A

    CHEMICAL COMMUNICATIONS   59 ( 12 )   1657 - 1660   2023年2月   ISSN:1359-7345 eISSN:1364-548X

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Chemical Communications  

    The polyion complex-based supramolecular self-assembly of hexagonal nanosheets was achieved via the complexation of a PEGylated block catiomer with ATP and other polyphosphate-containing small molecules. The formation of hexagonal nanosheets required the presence of a polyethylene glycol block and α-helix formation in the catiomer block, which was induced by complexation with the polyphosphate moiety.

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  • Characterization of polypropyleneimine as an alternative transfection reagent, 査読 国際誌

    R. Saeki, S. Kobayashi, R. Shimazui, T. Nii, A. Kishimura, T. Mori, M. Tanaka, Y. Katayama

    Anal. Sci.   2023年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    リポジトリ公開URL: https://hdl.handle.net/2324/7178534

  • Development of Polynucleotide-loaded Nanoparticles for the Regulation of Intracellular Nucleotide Levels

    Liu, YW; Kishimura, A; Katayama, Y; Mori, T

    CHEMISTRY LETTERS   51 ( 11 )   1037 - 1039   2022年11月   ISSN:0366-7022 eISSN:1348-0715

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    出版者・発行元:Chemistry Letters  

    Polynucleotide-loaded nanoparticles are potentially useful for the regulation of intracellular nucleotide levels. Polyadenylic acid and polyguanylic acid were used as model compounds for encapsulation into poly(lactic-co-glycolic acid) nanoparticles. The properties of the produced nanoparticles, as well as the effect of the polynucleotide molecular size on encapsulation into the nanoparticles were investigated.

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  • Development of Polynucleotide-loaded Nanoparticles for the Regulation of Intracellular Nucleotide Levels 招待 査読 国際誌

    Y. Liu, A. Kishimura, Y. Katayama, T. Mori

    Chem. Lett.   51   1037 - 1039   2022年10月

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  • Dietary-protein sources modulate host susceptibility to Clostridioides difficile infection through the gut microbiota 招待 査読 国際誌

    K. Yakabe, S. Higashi, M. Akiyama, H. Mori, T. Murakami, A. Toyoda, Y. Sugiyama, S. Kishino, K. Okano, A. Hirayama, A. Gotoh, S. Li, T. Mori, T. Katayama, J. Ogawa, S. Fukuda, K. Hase, and Y.-G. Kim

    Cell Rep.   40   111332   2022年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Dietary-protein sources modulate host susceptibility to <i>Clostridioides difficile</i> infection through the gut microbiota

    Yakabe, K; Higashi, S; Akiyama, M; Mori, H; Murakami, T; Toyoda, A; Sugiyama, Y; Kishino, S; Okano, K; Hirayama, A; Gotoh, A; Li, SY; Mori, T; Katayama, T; Ogawa, J; Fukuda, S; Hase, K; Kim, YG

    CELL REPORTS   40 ( 11 )   111332   2022年9月   ISSN:2211-1247

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    記述言語:英語   出版者・発行元:Cell Reports  

    Clostridioides difficile causes nosocomial antibiotic-associated diarrhea on a global scale. Susceptibility to C. difficile infection (CDI) is influenced by the composition and metabolism of gut microbiota, which in turn are affected by diet. However, the mechanism underlying the interplay between diet and gut microbiota that modulates susceptibility to CDI remains unclear. Here, we show that a soy protein diet increases the mortality of antibiotic-treated, C. difficile-infected mice while also enhancing the intestinal levels of amino acids (aas) and relative abundance of Lactobacillus genus. Indeed, Ligilactobacillus murinus-mediated fermentation of soy protein results in the generation of aas, thereby promoting C. difficile growth, and the process involves the anchored cell wall proteinase PrtP. Thus, mutual interaction between dietary protein and the gut microbiota is a critical factor affecting host susceptibility to CDI, suggesting that dietary protein sources can be an important determinant in controlling the disease.

    DOI: 10.1016/j.celrep.2022.111332

    Web of Science

    Scopus

    PubMed

  • Preparation of a PEGylated liposome that co-encapsulates L-arginine and doxorubicin to achieve a synergistic anticancer effect 招待 査読 国際誌

    H. Feng, J.-H. Kang, S. Qi, A. Kishimura, T. Mori, Y. Katayama

    RSC Adv.   11   34101 - 34106   2022年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Nanostructure Control of an Antibiotic-Based Polyion Complex Using a Series of Polycations with Different Side-Chain Modification Rates. 国際誌

    Asmariah Ahmad, Teruki Nii, Takeshi Mori, Yoshiki Katayama, Masanori Toyofuku, Akihiro Kishimura

    Macromolecular rapid communications   e2200316   2022年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Developing nanovehicles for delivering antibiotics is a promising approach to overcome the issue of antibiotic resistance. This study aims to utilize a polyion complex (PICs) system for developing novel nanovehicles for polymyxin-type antibiotics, which are known as last resort drugs. The formation of antibiotic-based PIC nanostructures is investigated using colistimethate sodium (CMS), an anionic cyclic short peptide, and a series of block catiomers bearing different amounts of guanidinium moieties on their side chains. In addition, only the modified catiomer, and not the unmodified catiomer, self-assembles with CMS, implying the importance of the guanidine moieties for enhancing the interaction between the catiomer and CMS via the formation of multivalent hydrogen bonding. Moreover, micellar and vesicular PIC nanostructures are selectively formed depending on the ratio of the guanidine residues. Size-exclusion chromatography reveals that the encapsulation efficiency of CMS is dependent on the guanidinium modification ratio. The antimicrobial activity of the PIC nanostructures is also confirmed, indicating that the complexation of CMS in the PICs and further release from the PICs successfully occurs.

    DOI: 10.1002/marc.202200316

  • Comparative evaluation of natural killer cell-mediated cell killing assay based on the leakage of an endogenous enzyme or a pre-loaded fluorophore 招待 査読 国際誌

    K. Tanito, Y. Oshiro, H. Tagawa, A. Kishimura, T. Mori, Y. Katayama

    Anal. Sci.   37   1571-1575   2021年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • A FRET-based Protein Kinase Assay using Phos-tag-modified Quantum Dots and Fluorophore-labeled Peptides 招待 査読 国際誌

    T. Nobori, A. Kishimura, T. Mori, Y. Katayama

    Anal. Sci.   21   1361 - 1366   2021年10月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  • Effect of size and loading of retinoic acid in polyvinyl butyrate nanoparticles on amelioration of colitis 招待 査読 国際誌

    J. Li, Y. Mu, Y. Liu, A. Kishimura, T. Mori, Y. Katayama

    polymers   13   1472   2021年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • α-L-Arabinofuranosidase as an orthogonal enzyme for human cells 招待 査読 国際誌

    R. Kaneko, T. Oda, R. Yoshida, C. Tateishi, K. Tanito, T. Nii, A. Kishimura, N. Kamiya, T. Mori, Y. Katayama

    Chem. Lett.   50   1493 - 1495   2021年7月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  • Protein kinase C α-responsive gene carrier for cancer-specific transgene expression and cancer therapy 招待 査読 国際誌

    C. W. Kim, R. Toita, J.-H. Kang, T. Mori, A. Kishimura, Y. Katayama

    ACS Biomater. Sci. Eng.   7   2530 - 2537   2021年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Inducible Dynamic Behavior of Polyion Complex Vesicles by Disrupting Charge Balance 招待 査読 国際誌

    Y. Liu, T. Maruyama, Biplab KC, T. Mori, Y. Katayama, A. Kishimura

    Chem. Lett.   50   1034 - 1037   2021年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Synthesis and biological evaluation of a monocyclic Fc-binding antibody-recruiting molecule for cancer immunotherapy 招待 査読 国際誌

    K. Sasaki, K. Muguruma, R. Osawa, A. Fukuda, A. Taniguchi, A. Kishimura, Y. Hayashi, T. Mori, Y. Katayama

    RSC Med. Chem.   12   406 - 409   2021年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Effect of chloroacetyl modification on the suppression of dissociation of a fluorescent molecule from cells for antigen-specific cell staining 招待 査読 国際誌

    R. Kaneko, M. Kawamura, A. Kishimura, T. Mori, Y. Katayama

    Anal. Sci.   37   529 - 532   2021年1月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  • Effect of polyvinyl butyrate nanoparticles incorporated with immune suppressing vitamins on alteration of population of intestinal immune cells 査読 国際誌

    Y. Mu, Y. Kinashi, A. Kishimura, T. Mori, K. Hase, Y. Katayama

    Prog. Nat. Sci. Mater. Int.   30   707 - 70   2020年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Evaluation of Synergistic Effect of L-Arginine on the Anticancer Activity of Doxorubicin by Using Co-culture System 査読 国際誌

    H. Feng, A. Kishimura, T. Mori, Y. Katayama

    Anal Sci   36   1279 - 1283   2020年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Development of a novel molecular probe for the detection of liver mitochondrial redox metabolism 査読 国際誌

    Md. Z. Hosain, F. Hyodo, T. Mori, K. Takahashi, Y. Nagao, H. Eto, T. Nakaji, M. Murata, T. Akaboshi, M. Matsuo, Y. Katayama

    Sci. Rep   10   16489   2020年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • A lipid-based nanocarrier containing active vitamin D3 ameliorates NASH in mice via direct and intestine-mediated effects on liver inflammation 査読 国際誌

    Y. Mu, J. Li, J.-H. Kang, H. Eto, K. Zai, A. Kishimura, F. Hyodo, T. Mori, Y. Katayama

    Biol. Pharm. Bull.   43   1413 - 1420   2020年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Effect of an endothelin B receptor agonist on the tumor accumulation of nanocarriers 査読 国際誌

    H. Feng, L. T. Nam, T. Yoshikawa, A. Kishimura, T. Mori, Y. Katayama

    Biol. Pharm. Bull.   43   1301 - 1305   2020年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • ビタミンD3含有脂質ナノ粒子によるリーキーガットの改善に基づくNASHの治療(Therapeutic effect of lipid-based nanocarriers containing vitamin D3 on NASH by ameliorating the leaky gut)

    穆 云妹, 李 晋廷, Zai Khadijah, 岸村 顕広, 兵藤 文紀, 森 健, 片山 佳樹

    日本薬学会年会要旨集   140年会   27X - pm12S   2020年3月

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    記述言語:英語  

  • Signal amplification in flow cytometry for cell surface antigen analysis 招待 査読 国際誌

    T. Mori, Y. Katayama

    J. Biochem.   166   205 - 212   2019年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • 次世代ワクチンの基盤開発研究 インジェクタブルゲル抗原製剤を用いたアプローチ

    浅井 大輔, 福田 靖, 諸熊 一則, 船本 大起, 山口 優子, 森 健, 片山 佳樹, 柴山 恵吾, 中島 秀喜

    日本バイオマテリアル学会大会予稿集   41回   506 - 506   2019年11月

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    記述言語:日本語  

  • 次世代ワクチンの基盤開発研究 インジェクタブルゲル抗原製剤を用いたアプローチ

    浅井 大輔, 福田 靖, 諸熊 一則, 船本 大起, 山口 優子, 森 健, 片山 佳樹, 柴山 恵吾, 中島 秀喜

    日本バイオマテリアル学会大会予稿集   41回   506 - 506   2019年11月

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    記述言語:日本語  

  • Injectable polypeptide hydrogel depot system for assessment of the immuneresponse-inducing efficacy of sustained antigen release alone 招待 査読 国際誌

    D. Asai, T. Fukuda, K. Morokuma, D. Funamoto, Y. Yamaguchi, T. Mori, Y. Katayama, K. Shibayama, H. Nakashima

    Macromol. Biosci.   19   e1900167   2019年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • 西洋ワサビペルオキシダーゼを用いた膜タンパク質の蛍光標識における信号対雑音比の改善 招待 査読 国際誌

    森 健, 神野 健太, 織田 剛史, 片山 佳樹

    分析化学   68   961 - 964   2019年10月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  • Nanoparticle-Based Drug and Gene Delivery for Tumor Targeting 招待 査読 国際誌

    T. Mori, A. Kishimura, Y. Katayama,

    Material Matters   14.3   2019年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Synthesis of Transmembrane Molecules by Click Chemistry 招待 査読 国際誌

    W. Hatanaka, H. Takeuchi, M. Koga, T. Ryujin, A. Kishimura, Y. Katayama, S. Tsukiji, T. Mori

    Chem Lett   48   433 - 436   2019年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Rapid and continuous accumulation of nitric oxide-releasing liposomes in tumors to augment the enhanced permeability and retention (EPR) effect 招待 査読 国際誌

    T. Yoshikawa, Y. Mori, H. Feng, K. Q. Phan, A. Kishimura, J.-H. Kang, T. Mori, Y. Katayama

    Int. J. Pharm.   565   481 - 487   2019年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Folate receptor-specific cell-cell adhesion by using a folate-modified peptide-based anchor 招待 査読 国際誌

    H. Nagai, W. Hatanaka, M. Matsuda, A. Kishimura, Y. Katayama, T. Mori

    J. Biomater. Sci. Polym. Ed.   30   983 - 993   2019年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Regulation of inflammatory response of macrophages and induction of regulatory T cells by using retinoic acid-loaded nanostructured lipid carrier 招待 査読 国際誌

    K. Zai, N. Ishihara, H. Oguchi, M. Hirota, T. Mori, K. Hase, Y. Katayama

    Anal. Sci.   30   1 - 11   2019年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Preparation of complexes between ovalbumin nanoparticles and retinoic acid for efficient induction of tolerogenic dendritic cells 招待 査読 国際誌

    K. Zai, K. Yuzuriha, A. Kishimura, T. Mori, Y. Katayama

    Anal. Sci.   34   1243 - 1248   2018年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Non-covalent Coating of Liposome Surface with IgG through Its Constant Region 招待 査読 国際誌

    H. Sato, Y. Miyashita, K. Sasaki, A. Kishimura, T. Mori, Y. Katayama

    Chem. Lett.   47   770 - 772   2018年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Alkaline Phosphatase-Catalyzed Amplification of a Fluorescence Signal for Flow Cytometry 査読 国際誌

    T. Nobori, K. Tosaka, A. Kawamura, T. Joichi, K. Kamino, A. Kishimura, E. Baba, T. Mori, Y. Katayama

    Anal. Chem.   90   1059 - 1062   2018年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    フローサイトメトリーを高感度化するために、酵素反応を利用して細胞に対する蛍光標識の強度を増強させる方法を開発した

  • A Dual Alkylated Peptide-ligand Enhances Affinity to Human Serum Albumin 招待 査読 国際誌

    E. Nakahei, K. Takehara, H. Sato, K. Zai, A. Kishimura, T. Mori, Y. Katayama

    Anal. Sci.   34   501 - 504   2018年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Ligand-mediated Coating of Liposomes with Human Serum Albumin 招待 査読 国際誌

    H. Sato, E. Nakhaei, Elnaz, T. Kawano, M. Murata, A. Kishimura, T. Mori, Y. Katayama

    Langmuir   34   2324 - 2331   2018年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Ligand design for cancer imaging with long blood circulation and enhanced accumulation ability in tumors 査読 国際誌

    E. Nakhaei, C. W. Kim, D. Funamoto, H. Sato, Y. Nakamura, A. Kishimura, T. Mori, Y. Katayama

    Med. Chem. Commun.   8   1190 - 1195   2017年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Encapsulation of a nitric oxide-donor into a liposome to boost the enhanced permeation and retention (EPR) effect 査読 国際誌

    Y. Tahara, T. Yoshikawa, H. Sato, Y. Mori, Z. Hosain, A. Kishimura, T. Mori, Y. Katayama

    Med. Chem. Commun.   8   415 - 421   2017年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Enzyme-catalyzed amplification of fluorescent immunolabeling of a single cell for high-sensitive flow cytometry 査読

    Nobori Takanobu, Tosaka Kenta, Yamamoto Tatsuhiro, Kishimura Akihiro, Mori Takeshi, Katayama Yoshiki

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   254   2017年8月

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    記述言語:その他  

    Enzyme-catalyzed amplification of fluorescent immunolabeling of a single cell for high-sensitive flow cytometry

  • Development of Enzyme Loaded Polyion Complex Vesicle (PICsome): Thermal Stability of Enzyme in PICsome Compartment and Effect of Coencapsulation of Dextran on Enzyme Activity. 査読 国際誌

    Hengmin Tang, Yuki Sakamura, Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura

    Macromolecular bioscience   17 ( 8 )   2017年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Applications of enzymes are intensively studied, particularly for biomedical applications. However, encapsulation or immobilization of enzymes without deactivation and long-term use of enzymes are still at issue. This study focuses on the polymeric vesicles "PICsomes" for encapsulation of enzymes to develop a hecto-nanometer-scaled enzyme-loaded reactor. The catalytic activity of a PICsome-based enzyme nanoreactor is carefully examined to clarify the effect of compartmentalization by PICsome. Encapsulation by PICsome provides a stability enhancement of enzymes after 24 h incubation at 37 °C, which is particularly helpful for maintaining the high effective concentration of β-galactosidase. Moreover, to control the microenvironment inside the nanoreactor, a large amount of dextran, a neutral macromolecule, is encapsulated together with β-galactosidase in the PICsome. The resulting dextran-coloaded nanoreactor contributes to the enhancement of enzyme stability, even after exposure to 24 h incubation at -20 °C, mainly due to the antifreezing effect.

    DOI: 10.1002/mabi.201600542

  • Synergic modulation of the inflammatory state of macrophages utilizing anti-oxidant and phosphatidylserine-containing polymer-lipid hybrid nanoparticles. 査読 国際誌

    Md Zahangir Hosain, Kazuki Yuzuriha, Khadijah, Masafumi Takeo, Akihiro Kishimura, Yoshihiko Murakami, Takeshi Mori, Yoshiki Katayama

    MedChemComm   8 ( 7 )   1514 - 1520   2017年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Inflammatory activation of macrophages is a key factor in chronic inflammatory diseases such as ulcerative colitis. The excessive production of reactive oxygen species (ROS)/reactive nitrogen species (RNS) by macrophages causes oxidative stress during the inflammatory response and exaggerates inflammatory lesions in ulcerative colitis. Inhibition of the inflammatory activation of macrophages is a promising treatment for chronic inflammatory diseases. Here, we prepared self-filling polymer-lipid hybrid nanoparticles (PST-PLNPs) consisting of poly dl-lactic acid as a hydrophobic biodegradable polymer core encapsulating α-tocopherol (T) and phosphatidylserine (PS) both on the surface and interior of the particle. We confirmed the anti-inflammatory response of these hybrid nanoparticles in activated murine macrophages. PS has anti-inflammatory effects on macrophages by modulating the macrophage phenotype, while α-tocopherol is an antioxidant that neutralizes ROS. We found that PS-containing (PS-PLNPs) and PS plus α-tocopherol-containing (PST-PLNPs) polymer-lipid hybrid nanoparticles significantly increased the viability of lipopolysaccharide (LPS)-treated macrophages compared with phosphatidylcholine-containing PLNPs. PST-PLNPs had a better effect than PS-PLNPs, which was attributed to the synergy between PS and α-tocopherol. This synergic action of PST-PLNPs reduced NO and pro-inflammatory cytokine (IL-6) production and increased anti-inflammatory cytokine (TGF-β1) production when incubated with activated macrophages. Thus, these self-filling biodegradable polymer-lipid hybrid nanoparticles (PST-PLNPs) containing anti-oxidant and anti-inflammatory molecules might be potential alternative drug carriers to liposomes and polymeric nanoparticles for the treatment of chronic inflammatory diseases such as ulcerative colitis.

    DOI: 10.1039/c7md00174f

  • Efficient delivery of signal-responsive gene carriers for disease-specific gene expression via bubble liposomes and sonoporation, 査読 国際誌

    A. Tsuchiya, J.-H. Kang, T. Mori, Y. Naritomi, S. Kushio, T. Niidome, K. Tachibana, Y. Takahashi, Y. Negishi, Y. Oda, R. Suzuki, K. Maruyama, Y. Katayama

    Coll. Surf. B: Biointerfaces   160   60 - 64   2017年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Ligand design for cancer imaging with long blood circulation and enhanced accumulation ability in tumors 招待 査読 国際誌

    E. Nakhaei, C. W. Kim, D. Funamoto, H. Sato, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

    Med. Chem. Comm.   2017年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Facilitating the presentation of antigen peptides on dendritic cells for cancer immunotherapy using a polymer-based synthetic receptor 招待 査読 国際誌

    C. Li, M. Takeo, M. Matsuda, H. Nagai, S. Xizheng, W. Hatanaka, Akihiro Kishimura, Yoshiki Katayama, Takeshi Mori

    Med. Chem. Commun.   2017年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Modification of ligands for serum albumin on polyethyleneimine to stabilize polyplexes in gene delivery 招待 査読 国際誌

    Y. Nakamura, H. Sato, T. Nobori, H. Matsumoto, S. Toyama, T. Shuno, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

    J. Biomater. Sci. Polym. Ed.   2017年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Use of membrane potential to achieve transmembrane modification with an artificial receptor 招待 査読 国際誌

    W. Hatanaka, M. Kawaguchi, X. Sun, Y. Nagao, H. Ohshima, M. Hashida, Y. Higuchi, Akihiro Kishimura, Yoshiki Katayama, Takeshi Mori

    Bioconjugate Chem.   28   296   2017年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    細胞膜に膜貫通した状態で分子を修飾する方法を世界ではじめて開発した。

  • Encapsulation of a nitric oxide-donor into a liposome to boost the enhanced permeation and retention (EPR) effect 招待 査読 国際誌

    Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura, Y. Tahara

    Med. Chem. Commun   8   415   2017年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Fabrication of Dendrimer‐Based Polyion Complex Submicrometer‐Scaled Structures with Enhanced Stability under Physiological Conditions 招待 査読 国際誌

    K. Naoyama, Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura

    Macromol. Rapid Commun.   37   1087   2016年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Reversal of efflux of an anticancer drug in human drug-resistant breast cancer cells by inhibition of protein kinase Cα (PKCα) activity. 査読 国際誌

    Kim CW, Asai D, Kang JH, Kishimura A, Mori T, Katayama Y

    Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine   37 ( 2 )   1901 - 8   2016年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Reversal of efflux of an anticancer drug in human drug-resistant breast cancer cells by inhibition of protein kinase Cα (PKCα) activity.
    P-glycoprotein (Pgp) is a 170-kDa transmembrane protein that mediates the efflux of anticancer drugs from cells. Pgp overexpression has a distinct role in cells exhibiting multidrug resistance (MDR). We examined reversal of drug resistance in human MDR breast cancer cells by inhibition of protein kinase Cα (PKCα) activity, which is associated with Pgp-mediated efflux of anticancer drugs. PKCα activity was confirmed by measurement of phosphorylation levels of a PKCα-specific peptide substrate (FKKQGSFAKKK-NH2), showing relatively higher basal activity in drug-resistant MCF-7/ADR cells (84 %) than that in drug-sensitive MCF-7 cells (63 %). PKCα activity was effectively suppressed by the PKC inhibitor, Ro-31-7549, and reversal of intracellular accumulation of doxorubicin was observed by inhibition of PKCα activity in MCF-7/ADR cells compared with their intrinsic drug resistance. Importantly, increased accumulation of doxorubicin could enhance the therapeutic efficacy of doxorubicin in MDR cells significantly. These results suggest a potential for overcoming MDR via inhibition of PKCα activity with conventional anticancer drugs.

    DOI: 10.1007/s13277-015-3963-4

  • Synergy between phenotypic modulation and ROS neutralization in reduction of inflammatory response of hypoxic microglia by using phesphatidylserine and antioxidant containing liposomes 招待 査読 国際誌

    M Z Hosain, 森 健, 岸村 顕広, 片山 佳樹

    J. Biomater. Sci. Polym. Ed.   27   290   2016年1月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  • Suppression of atopic dermatitis in mice model by reducing inflammation utilizing phosphatidylserine-coated biodegradable microparticles 査読 国際誌

    P. Kumar, M. Z. Hosain, J.-H. Kang, M. Takeo, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

    J. Biomater. Sci. Polym. Ed.   26   1465   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Surface engineering of macrophages with nucleic acid aptamers for circulating tumor cell capture 招待 査読 国際誌

    S. Sugimoto, R. Moriyama, Takeshi Mori, Y. Iwasaki

    Chem. Commun.   51   17428   2015年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Tumor accumulation of protein kinase-responsive gene carrier/DNA polyplex stabilized by alkanethiol for intravenous injection 査読 国際誌

    K. Li, H. Sato, C.W. Kim, Y. Nakamura, G.X. Zhao, D. Funamoto, T. Nobori, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

    J. Biomater. Sci. Polym. Ed.   26   657   2015年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Tumor accumulation of protein kinase-responsive gene carrier/DNA polyplex stabilized by alkanethiol for intravenous injection 査読

    Kai Li, Hikari Sato, Chan Woo Kim, Yuta Nakamura, Guo Xi Zhao, Daiki Funamoto, Takanobu Nobori, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

    Journal of Biomaterials Science, Polymer Edition   26 ( 11 )   657 - 668   2015年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We synthesized polymeric gene carriers consisting of poly-L-lysine (PLL) main chain modified both with substrate peptide for protein kinase C (PKC) and alkanethiol (pentadecanethiol). Due to the grafted substrate peptide, the polyplex prepared from these carriers is expected to show gene expression triggered by the phosphorylation of the peptide by intracellular PKC. The modified alkanethiol on the main chain stabilized the polyplex both via disulfide crosslinking and hydrophobic interaction. The polyplex found to show gene expression in vitro when the alkanethiol content in the main chain was enough low (4-mol%-modification of PLLs ε-amine group) to minimize cytotoxic effect. Even though the content of alkanethiol is low, the polyplex had significant stability in a model serum solution and showed longer blood circulation in vivo. The polyplex clearly accumulated in tumor after intravenous injection.

    DOI: 10.1080/09205063.2015.1054922

  • Optimum design of amphiphilic polymers bearing hydrophobic groups for both cell surface ligand presentation and intercellular cross-linking 査読

    Masafumi Takeo, Cuicui Li, Masayoshi Matsuda, Hiroko Nagai, Wataru Hatanaka, Tatsuhiro Yamamoto, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

    Journal of Biomaterials Science, Polymer Edition   26 ( 6 )   353 - 368   2015年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Amphiphilic polymers bearing hydrophobic alkyl groups are expected to be applicable for both ligand presentation on the cell surface and intercellular crosslinking. To explore the optimum design for each application, we synthesized eight different acyl-modified dextrans with varying molecular weight, alkyl length, and alkyl modification degree. We found that the behenate-modified polymers retained on the cell surface longer than the palmitate-modified ones. Since the polymers were also modified with biotin, streptavidin can be presented on the cell surface through biotin-streptavidin recognition. The duration of streptavidin on the cell surface is longer in the behenate-modified polymer than the palmitate-modified one. As for the intercellular crosslinking, the palmitate-modified polymers were more efficient than the behenate-modified polymers. The findings in this research will be helpful to design the acyl-modified polymers for the cell surface engineering.

    DOI: 10.1080/09205063.2015.1007414

  • Optimum design of amphiphilic polymers bearing hydrophobic groups both for cell surface ligand presentation and intercellular cross-linking 査読 国際誌

    M. Takeo, C. Li, M. Matsuda, H. Nagai, W. Hatanaka, T. Yamamoto, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

    J. Biomater. Sci. Polym. Ed.   26   353   2015年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Tandemly Repeated Peptide for Cancer-specific Gene Carrier Prepared by Native Chemical Ligation 査読 国際誌

    D Funamoto, D Asai, CW Kim, Y Nakamura, EK Lee, T Niidome, Takeshi Mori, Yoshiki Katayama

    Chem Lett   44   474 - 476   2015年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Antibody internalization into living cells via crosslinker-mediated endocytosis 査読

    Daiki Funamoto, Daisuke Asai, Kazuki Sato, Yoko Yamaguchi, Chan Woo Kim, Hikari Sato, Elnaz Nakhaei, Shingo Matsumoto, Takuma Yoshikawa, Koichi Sasaki, Tatsuhiro Yamamoto, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

    Chemistry Letters   44 ( 4 )   468 - 470   2015年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We reported here an alternative strategy of antibody internalization by crosslinker-mediated endocytosis. This cross-linker consists of IgG binding peptide and folic acid as cancer targeting ligands toward the folate receptor, which highly expresses in many cancer cells surface. This crosslinker successfully facilitated antibody internalization into cancer cell by the folate receptor-mediated endocytosis.

    DOI: 10.1246/cl.141157

  • Utilization of a PNA-peptide conjugate to induce a cancer protease-responsive RNAi effect 査読

    Eun Kyung Lee, Chan Woo Kim, Hiroyuki Kawanami, Akihiro Kishimura, Takuro Niidome, Takeshi Mori, Yoshiki Katayama

    RSC Advances   5 ( 104 )   85816 - 85821   2015年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Small interfering RNA (siRNA) is regarded as a promising tool for cancer therapy because of the wide applicability to various cancer-related genes. However, non-specific delivery of siRNA is one of the major causes of adverse effects. To access the issue, here we designed a new siRNA system which turns on RNAi responding to a cancer cell-specific protease, cathepsin B. The system uses a peptide nucleic acid (PNA)-peptide conjugate to provide this protease-responsive activation. The PNA-peptides were found to form hybrids with double-stranded RNAs with complementary protruding regions, which then affected the susceptibility of dsRNA to Dicer. The dsRNA/PNA-peptide hybrids were activated in cancer cells with a high cathepsin B activity to show RNAi.

    DOI: 10.1039/c5ra17737e

  • Tandemly repeated peptide for cancer-specific gene carrier prepared by native chemical ligation 査読

    Daiki Funamoto, Daisuke Asai, Chan Woo Kim, Yuta Nakamura, Eun Kyung Lee, Takanobu Nobori, Takuro Niidome, Takeshi Mori, Yoshiki Katayama

    Chemistry Letters   44 ( 4 )   474 - 476   2015年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We reported here a preparation of a cancer-specific gene carrier by native chemical ligation of a substrate peptide of protein kinase Cα (PKCα), which highly expresses in many types of cancer. This carrier is a tetramer of the substrate peptide connected with adequate spacer to maintain reactivity toward PKCα. The carrier successfully regulated the reporter gene expression responding to the phosphorylation of its serine residues.

    DOI: 10.1246/cl.141121

  • Surface engineering of macrophages with nucleic acid aptamers for the capture of circulating tumor cells 査読

    Shunsuke Sugimoto, Rui Moriyama, Takeshi Mori, Yasuhiko Iwasaki

    Chemical Communications   51 ( 98 )   17428 - 17430   2015年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In order to enhance the interactions between macrophages and cancer cells, thiol-terminated nucleic acid aptamers were immobilized on methacryloyl-functionalised carbohydrates of macrophages. The adhesion of cancer cells on the surface modified macrophages was significantly accelerated.

    DOI: 10.1039/c5cc06211j

  • Short peptide motifs for long-lasting anchoring to the cell surface 査読

    Masayoshi Matsuda, Wataru Hatanaka, Masafumi Takeo, Chan Woo Kim, Takuro Niidome, Tatsuhiro Yamamoto, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

    Bioconjugate Chemistry   25 ( 12 )   2134 - 2143   2014年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A rational design strategy has been developed for the construction of stable peptide-based anchors for the efficient modification of cell surfaces. Six types of peptide composed of five residues with divalent hydrophobic groups have been designed using this new strategy. Among them, a peptide with a sequence of NBD-Lys-Lys(X)-Lys-Lys-Lys(X)-NH2 (NBD: fluorophore, Lys(X): N-ε-palmitoyl-L-lysine) was found to show the highest modification efficacy and longevity in culture medium. The good performance of this peptide was attributed to (1) its high aqueous solubility, which allowed it to partition from the medium to the cell surface, and (2) the high binding affinity of the saturated palmitoyl groups to the cell membrane. We found that the distribution of the peptide was affected by recycling endosome, which enabled the representation of the peptide following its endocytotic disappearance from the cell membrane. Biotin was also presented on the cell surface using this peptide-based anchor to examine its recognition by streptavidin. The efficacy of the recognition process increased as the length of the oligoethylene glycol spacer increased, indicating that it was necessary for the biotin tag to move away from the membrane glycoproteins on the cell surface to facilitate its efficient recognition by streptavidin. (Figure Presented).

    DOI: 10.1021/bc500465j

  • Effect of peptide content on the regulation of transgene expression by protein kinase Cα-responsive linear polyethylenimine-peptide conjugates 査読

    Riki Toita, Jeong Hun Kang, Chan Woo Kim, Shujiro Shiosaki, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

    Colloids and Surfaces B: Biointerfaces   123   123 - 129   2014年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We examined a series of linear polyethylenimine (LPEI)-based nanocarriers that activate transgene expression in response to cancer-specific protein kinase Cα (PKCα). Eight types of LPEI-peptide conjugate differing in peptide content and number were synthesized using click chemistry. The conjugates could form polyplexes with pDNA through electrostatic interaction, but the degree of pDNA condensation, sizes, and surface charges of the resulting polyplexes depended on the pendant-peptide content and number. None of the polyplexes showed significant cytotoxicity toward human hepatoma cells (HepG2). Furthermore, pendant peptide content and number markedly affected transgene activation in response to PKCα. To achieve an all-or-none response to PKCα, we determined the optimum peptide content and number in LPEI-peptide conjugates as ≈6. mol% and ≈40. peptides/conjugate.

    DOI: 10.1016/j.colsurfb.2014.09.004

  • Hydrophobic cavity formed by oligopeptide for doxorubicin delivery based on dendritic poly(L-lysine) 査読

    Takuro Niidome, Hisayo Yamauchi, Kayo Takahashi, Kenshiro Naoyama, Kazuto Watanabe, Takeshi Mori, Yoshiki Katayama

    Journal of Biomaterials Science, Polymer Edition   25 ( 13 )   1362 - 1373   2014年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    To deliver anti-cancer drugs to tumors, a hydrophobic cavity was prepared in the dendritic molecule, dendritic poly(L-lysine) of sixth generation (KG6), which was used as a drug carrier. The dendritic molecule was modified with polyethylene glycol (PEG)-linked hydrophobic penta-phenylalanine or penta-Alanine. The hydrophobic cavity was formed between the KG6 and PEG chains. The penta-phenylalanine peptide was better in encapsulating doxorubicin (DOX) in the cavity compared with penta-Alanine. The loaded DOX was slowly released from the cavity, and it depended on pH. After intravenous injection, the DOX-loaded dendrimers accumulated in the tumor by the enhanced permeability and retention effect, and showed significant suppression of tumor growth without loss of body weight. These results indicate that hydrophobic oligopeptides can be used for forming a hydrophobic cavity in a dendritic molecule for delivery of anti-cancer drugs to tumor sites.

    DOI: 10.1080/09205063.2014.938979

  • Nitroxyl radicals-modified dendritic poly(l -lysine) as a contrast agent for Overhauser-enhanced MRI 査読

    Takuro Niidome, Risa Gokuden, Kazuto Watanabe, Takeshi Mori, Tatsuya Naganuma, Hideo Utsumi, Kazuhiro Ichikawa, Yoshiki Katayama

    Journal of Biomaterials Science, Polymer Edition   25 ( 13 )   1425 - 1439   2014年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Overhauser-enhanced magnetic resonance imaging (OMRI), which is a double resonance technique, creates images of free radical distribution in animals by enhancing the water proton signal intensity by the overhauser effect. In this study, we constructed a contrast agent by combining PROXYL groups that have nitroxyl radicals with PEG-modified dendritic poly(l-lysine) that accumulates in the tumor by enhanced permeability and retention (EPR) effect. Addition of the PROXYL groups at the PEG chains termini on KG6 was advantageous in OMRI, because the ESR signal of the nitroxyl radical was maintained without decay caused by mobility restriction, even if the PROXYL groups were attached at 25 mol% on one molecule. After intramuscular injection of the molecule modified at 25 mol%, that is, PR25-PEG-KG6, a significant OMRI signal was observed at the injected site. However, no signal was detected in the tumor after intravenous injection of PR25-PEG-KG6 to a tumor-bearing mouse, although PR 25-PEG-KG6 itself accumulated in the tumor. The reason was that the nitroxyl radicals were immediately reduced in the blood after the injection, suggesting that use of stable nitroxyl radicals will enable detection of tumors by OMRI after intravenous injection.

    DOI: 10.1080/09205063.2014.943538

  • Change in Overhauser Effect-enhanced MRI Signal in Response to uPA Highly Expressing in Tumor

    T. Niidome, N. Chijiiwa, T. Yamasaki, K. Yamada, Takeshi Mori, T. Naganuma, H. Utsumi, H. Utsumi, Yoshiki Katayama

    Chem Lett   43   999   2014年8月

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    記述言語:英語  

  • A liposome reversibly coated with serum albumin

    H. Sato, Y. Nakamura, E. Nakhaei, D.Funamoto, C. W. Kim, T. Yamamoto, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

    Chem. Lett.   43   1481   2014年6月

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    記述言語:英語  

  • PNA-tagged peptide microarrays for ratiometric activity detection of cellular protein kinases 査読 国際誌

    H. Ikeda, Y. Yayama, A. Hata, J. Kamimotoa, T. Yamamoto, Takeshi Mori, Yoshiki Katayama

    Anal. Sci.   30   631   2014年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Fluorescent polyion complex nanoparticle that incorporates an internal standard for quantitative analysis of protein kinase activity. 査読 国際誌

    Takanobu Nobori, Shujiro Shiosaki, Takeshi Mori, Riki Toita, Chan Woo Kim, Yuta Nakamura, Akihiro Kishimura, Takuro Niidome, Yoshiki Katayama

    Bioconjugate chemistry   25 ( 5 )   869 - 72   2014年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We demonstrate a polyion complex (PIC) nanoparticle that contains both a responsive fluorophore and an "internal standard" fluorophore for quantitative measurement of protein kinase (PK) activity. The PK-responsive fluorophore becomes more fluorescent with PK-catalyzed phosphorylation of substrate peptides incorporated in the PIC, while fluorescence from the internal standard remains unchanged during phosphorylation. This new concept will be useful for quantitative PK assays and the discovery of PK inhibitors.

    DOI: 10.1021/bc500142j

  • Microarray Technologies for Intracellular Kinome Analysis 招待 査読 国際誌

    T. Yamamoto, Takeshi Mori, Yoshiki Katayama

    Current Med. Chem.   21   2542   2014年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • A fluorescent polyion complex nanoparticle that incorporates an internal standard for quantitative analysis of protein kinase activity

    T. Nobori, S. Shiosaki, Takeshi Mori, R. Toita, C. W. Kim, Y. Nakamura, A. Kishimura, T. Niidome, Yoshiki Katayama

    Bioconjugate Chem.   25   869   2014年5月

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    記述言語:英語  

  • A sustained controlled release formulation of soil nitrogen based on nitrate-layered double hydroxide nanoparticle material 査読

    Mohamed R. Berber, Inas H. Hafez, Keiji Minagawa, Takeshi Mori

    JOURNAL OF SOILS AND SEDIMENTS   14 ( 1 )   60 - 66   2014年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Purpose Nitrate-layered double hydroxide material (nitrate-LDH) matrix can be considered as a potential formulation of delivering nitrogen into soil in a sustained manner.
    Materials and methods The nitrate-LDH matrix was formulated by a co-precipitation technique and subsequently characterized by scanning electron microscopy, X-ray analysis, and infrared spectroscopy. The release of nitrate was monitored in different buffer mediums: buffer A as a simulated acidic soil solution and buffer B as a simulated neutral soil solution.
    Results and discussion The stability of nitrate-LDH against thermal decomposition was evaluated by thermal gravimetric analysis. The nitrate-LDH supported a sustained controlled release process of nitrate during 16 days into acidic soil at 15 degrees C, while the release was continued to 20 days into neutral soil at the same temperature. The increase of soil temperature slightly enhanced the release of nitrate.
    Conclusions We offered a potential management strategy of soil nitrogen leaching process. The nitrate form of layered double hydroxide material was used as a nitrogen fertilizer in order to monitor the release of nitrate anion into soil at different conditions.

    DOI: 10.1007/s11368-013-0766-3

  • Effect of composition and stereoregularity on phase-transition behavior of aqueous N-ethylacrylamide/N-n-propylacrylamide copolymer solutions 査読 国際誌

    T. Hirano, A. Ono, H. Yamamoto, Y. Maeda, Takeshi Mori, M. Oshimura, K. Ute

    Polymer   54   56015608   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Cancer-specific gene carriers responding to cancer microenvironment: Acidosis and hyper-activated protein kinases

    Satoshi Kushio, Akira Tsuchiya, Yuta Nakamura, Takanobu Nobori, Chan Woo Kim, Guo Xi Zhao, Taiki Funamoto, Eun Kyung Lee, Takuro Niidome, Takeshi Mori, Yoshiki Katayama

    Biomedical Engineering - Applications, Basis and Communications   25 ( 5 )   2013年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Protein kinase (PK)-responsive gene carriers modified with polyethylene glycol (PEG) chains using an acid-labile linker were developed. These carriers were obtained by modifying the PEG chains and substrate peptides for the PKs (PKA or PKCα) on the branched polyethyleneimine main chain. Polyplexes formed from these carriers and plasmid DNA (pDNA) were stably dispersed under neutral pH medium. The polyplexes were also taken up by cells on the release of the PEG chains under the slightly acidic extracellular pH associated with cancer cells. The polyplexes taken up by cells resulted in gene expression when the substrate peptides were phosphorylated by the intracellular PKs to release pDNA from the polyplexes. These novel gene carriers are expected to be promising for cancer-specific gene therapy via intravenous administration. © 2013 National Taiwan University.

    DOI: 10.4015/S101623721340005X

  • A protein kinase assay based on FRET between quantum dots and fluorescently-labeled peptides 査読 国際誌

    Takeshi Mori, S. Shiosaki, T. Nobori, R. Toita, Y. Nakamura, C. W. Kim, T. Yamamoto, T. Niidome, Yoshiki Katayama

    Chem. Commun.   49   5592 - 5594   2013年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Kinase Activity of Protein Kinase Calpha in Serum as a Diagnostic Biomarker of Human Lung Cancer 査読 国際誌

    J.-H. Kang, Takeshi Mori, H. Kitazaki, T. Niidome, K. Takayama, Y. Nakanishi, Yoshiki Katayama

    Anticancer   33   485 - 488   2013年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Kinase Activity of Protein Kinase Calpha in Serum as a Diagnostic Biomarker of Human Lung Cancer 査読 国際誌

    J.-H. Kang, Takeshi Mori, H. Kitazaki, T. Niidome, K. Takayama, Y. Nakanishi, Yoshiki Katayama

    Anticancer   33   485 - 488   2013年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Rapid and Selective determination of free chlorine in aqueous solution using electrophilic addition to styrene by gas chromatography/mass spectrometry 査読 国際誌

    K. Wakigawa, A. Gohda, S. Fukushima, Takeshi Mori, Yoshiki Katayama

    Talanta   203   81 - 85   2013年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Thermal enhancement of gene transfection in tumor cells mediated by the photothermal effect of gold nanorods 査読 国際誌

    Y. Sakamura, M. Yoshiura, H. Tang, Takeshi Mori, Yoshiki Katayama, T. Niidome

    Chem. Lett.   42   767 - 768   2013年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Uniform nanoparticles of hydrotalcite-like materials and their textural properties at optimized conditions of urea hydrothermal treatment 査読 国際誌

    M. R. Berber, I. H. Hafez, K. Minagawa, Takeshi Mori, M. Katoh, M. Tanaka

    J Mol Struct   1033   104 - 112   2013年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • A polyion complex nanogel. 査読 国際誌

    Masafumi Takeo, Takeshi Mori, Takuro Niidome, Shinichi Sawada, Kazunari Akiyoshi, Yoshiki Katayama

    Journal of colloid and interface science   390 ( 1 )   78 - 84   2013年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Here, we synthesized dextrans modified with trivalent cationic or anionic groups. Aqueous solutions of the cationic and anionic dextrans were then mixed resulting in the formation of polyion complex nanogels (PIC-NGs), which have physically crosslinked salt bridges formed between the cationic and anionic groups. To prepare PIC-NGs in high yield, the content of ionic groups in the cationic and anionic dextrans should be low to avoid interparticle salt bridge formation. The structure of the PIC-NGs is easily affected by the ionic strength of the solution because of shielding of the charges in the ionic groups. However, the conversion of a small amount of the physical crosslinks to covalent crosslinks significantly improved the stability of the PIC-NGs. The covalent crosslinking also stabilized the PIC-NGs against pH change, which will destabilize the salt bridges. These results indicated that the conversion of the small amount of physical crosslinks to covalent crosslinks stabilized the other salt bridges.

    DOI: 10.1016/j.jcis.2012.09.015

  • An efficient strategy of managing irrigation water based on formulating highly absorbent polymer-inorganic clay composites 査読

    Mohamed R. Berber, Inas H. Hafez, Keiji Minagawa, Masami Tanaka, Takeshi Mori

    JOURNAL OF HYDROLOGY   470   193 - 200   2012年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The management of irrigation water presents a great challenge for the agriculture field. In view of increasing soil water-holding capacity and increasing water-use efficiency, an efficient strategy of managing irrigation water based on formulating highly absorbent polymer-inorganic clay composite (polyacrylic acid-layered double hydroxide: PAA-LDH) was offered. The PAA-LDH composite was synthesized by an incorporation/in situ polymerization technique. Scanning electron microscopy, X-ray analysis and infrared spectroscopy were used to confirm the composite structure. The thermal gravimetric analysis was applied to investigate the polymer thermal stability after the composite formation. The irrigation experiments were conducted in a wooden soil box with a transparent plexiglas side by using a subsurface drip irrigation system. The X-ray patterns and infrared spectra confirmed the incorporation of acrylic acid monomer (AA) into the gallery of LDH. The SEM images emphasized the composite structure of PAA-LDH and indicated its ability to absorb and keep water. The stability of PAA was promoted against the thermal decomposition after the composite formation. The composite structure of PAA-LDH worked as water barrier and secondary water source during the irrigation process. The soil moisture distribution patterns were enhanced after the application of PAA-LDH composites as a soil conditioner. (C) 2012 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.jhydrol.2012.08.051

  • A colorimetric assay of protein kinase activity based on peptide-induced coagulation of gold nanorods 査読 国際誌

    Takeshi Mori

    J. Collod. Interf. Sci. B: Biointerfaces   99   2012年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Conversion of rod-shaped gold nanoparticles to spherical forms and their effect on biodistribution in tumor-bearing mice. 査読 国際誌

    Yasuyuki Akiyama, Takeshi Mori, Yoshiki Katayama, Takuro Niidome

    Nanoscale research letters   7 ( 1 )   565 - 565   2012年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Gold nanorods that have an absorption band in the near-infrared region and a photothermal effect have been used as nanodevices for near-infrared imaging and thermal therapy. Choice of the optimal shape of gold nanorods which relates optical properties and in vivo biodistribution is important for their applications. In the present study, to investigate the relationship between the shape of gold nanorods and their biodistribution after intravenous injection, we first prepared two types of gold nanorods that had distinct aspect ratios but had the same volume, zeta potential, and PEG density on the gold surface. Biodistributions of the two types of gold nanorods after intravenous injection into tumor-bearing mice were then compared. Although a slight difference in accumulation in the spleen was observed, no significant difference was observed in the liver, lung, kidney, and tumors. These results suggest that biodistribution of the gold nanorods in the aspect ratio range of 1.7 to 5.0, diameter of 10 to 50 nm, and volume of approximately 4 × 103 nm3 was dependent mainly on surface characteristics, PEG density, and zeta potential.

    DOI: 10.1186/1556-276X-7-565

  • Controlled-Release System Mediated by a Retro Diels-Alder Reaction Induced by the Photothermal Effect of Gold Nanorods 査読 国際誌

    H. Fukushima, Takuro Niidome, S. Yamashita, Takeshi Mori, Yoshiki Katayama, Yasuro Niidome

    Langmuir   27   2012年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Phase Behavior of Aqueous Solutions of Copolymers of N,N’-Diisopropylfumaramide and N-Isopropylacrylamide: Effect of the Density of Side Chains 査読 国際誌

    A. Hashidzume, A. Matsumoto, T. Mori, T. Shikata, T. Sato

    Langmuir   28   2012年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Gene carrier showing all-or-none response to cancer cell signaling 査読 国際誌

    Takeshi Mori, Yoshiki Katayama, Riki Toita

    J. Am. Chem. Soc.   134   15410 - 15417   2012年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In this work we designed a novel nano carrier, a linear polyethylenimine (LPEI)-peptide conjugate, for cancerspecific expression of transgenes. The conjugate was easily synthesized by using a click chemistry scheme orthogonal to the reactive side groups of the peptide, which is the substrate of protein kinase Cα (PKCα). Polyplexes of the conjugates with plasmid DNA (pDNA) were intact and stably dispersed even in the presence of cell lysate. Despite this stability, the polyplexes readily dissociated upon phosphorylation of the grafted peptides by PKCα. Because of its endosomal escape ability and adequate susceptibility to PKCα, the polyplexes
    showed an all-or-none type response to PKCα activity in transgene expression in vitro. The polyplexes achieved cancer tissuespecific
    transgene expression even for a tumor with a relatively low PKCα activity. Thus the LPEI−peptide conjugate has high potential as a nanocarrier for cancer-targeted gene therapy.

  • Fluorometric detection of protein kinase Calpha activity based on phosphorylation-induced dissociation of a polyion complex 査読 国際誌

    Takeshi Mori

    Anal. Biochem.   424   2012年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Creating a unique environment for selecting reactive enzymes with DNA: ‘Sticky’ binding of oligocation-grafted polymers to DNA 査読 国際誌

    H. Tanaka, T. Mori, T. Niidome, Y. Katayama

    Bioorg. Med. Chem.   20   2012年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Target delivery and controlled release of the chemopreventive drug sulindac by using an advanced layered double hydroxide nanomatrix formulation system 査読

    Keiji Minagawa, Mohamed R. Berber, Inas H. Hafez, Takeshi Mori, Masami Tanaka

    JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE   23 ( 4 )   973 - 981   2012年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Target delivery and controlled release of the chemopreventive drug sulindac that possesses low water solubility present a great challenge for its pharmaceutical industry. Here, we offered an advanced nanomatrix formulation system of sulindac based on layered double hydroxide materials. The X-ray analysis and infrared spectroscopy confirmed the incorporation of sulindac into the gallery of the layered double hydroxides. The incorporation ratios of sulindac were recorded to be 45, 31 and 20 for coprecipitation, anion-exchange and reconstruction techniques, respectively. The scanning electron microscopy showed a nanomatrix-structure of similar to 50 nm. The release studies of sulindac-nanomatrix showed a 96% controlled release at the small intestine solution during 3 h(s), indicating an enhancement in the dissolution profile of sulindac after the matrix formation. The layered structure of the matrix supplied sulindac with a well-ordered structure and a relatively hydrophobic microenvironment that controlled the guest hydrolysis and reactivity during the release process. The laminar structure of layered double hydroxides offered a safe preservation for sulindac against photodecarboxylation, and enhanced the drug thermal stability from 190 to 230A degrees C. The ionic electrostatic interaction of sulindac through its acidic group with layered double hydroxides demolished the gastrointestinal ulceration.

    DOI: 10.1007/s10856-012-4566-x

  • Sequential release of single-stranded DNAs from gold nanorods triggered by near-infrared light irradiation

    Takeshi Mori

    Chem. Lett.   41   711 - 712   2012年4月

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    記述言語:英語  

  • Conversion of rod-shaped gold nanoparticles to spherical forms and their effect on biodistribution in tumor-bearing mice

    Takeshi Mori

    Nanoscale Res. Lett.   7   565   2012年4月

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    記述言語:英語  

  • Phase Behavior of Aqueous Solutions of Copolymers of N,N’-Diisopropylfumaramide and N-Isopropylacrylamide: Effect of the Density of Side Chains

    A Hashidzume, A. Matsumoto, T. Shikata, Takeshi Mori, T. Sato

    Langmuir   2012年4月

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    記述言語:英語  

  • Improvement in the colloidal stability of protein kinase-responsive polyplexes by PEG modification

    Takeshi Mori

    J. Biomed. Mater. Res. Part A   100   1136 - 1141   2012年4月

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    記述言語:英語  

  • Improvement in the colloidal stability of protein kinase-responsive polyplexes by PEG modification 査読 国際誌

    A. Tsuchiya, Y. Naritomi, S. Kushio, J.-H. Kang, M. Murata, M. Hashizume, T. Mori, T. Niidome, Y. Katayama

    J. Biomed. Mater. Res. Part A   100   2012年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Kinome Analysis Using Microarray of Peptide Substrates 査読

    Takeshi Mori, Jumpei Kamimoto, Yoshiki Katayama

    BUNSEKI KAGAKU   61 ( 3 )   185 - 191   2012年3月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

    Protein kinases (PKs) play crucial roles in intracellular signal transduction and they are one of the representative targets of drug development because their hyper-activation is related to many diseases including cancer. Kinomics is a technique to evaluate the activities of whole cellular PKs and will be important for drug development and diagnosis for personalized medicine. Peptide microarray is one of the representative methods to evaluate kinomics. We have proposed several techniques to improve the detection of peptide microarrays and have achieved quantitative evaluation of cellular PKs based on both fluorescence and SPR detection. We have also succeeded in screening of highly reactive substrate peptides of target PKs by using our microarrays.

  • Biodistribution and tumor localization of PEG-modified dendritic poly(L-lysine) oligonucleotide complexes 査読 国際誌

    R. Kurihara, D. Pissuwan, T. Mori, Y. Katayama, T. Niidome

    J. Biomater. Sci.-Polym. Ed.   23   2012年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Correlation between phosphorylation ratios by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and radioactivities by radioactive assay. 査読 国際誌

    Akira Tsuchiya, Daisuke Asai, Jeong-Hun Kang, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

    Analytical biochemistry   421 ( 2 )   773 - 5   2012年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    To investigate the correlation between the counts per minute (CPM) by radioactivity assay and the phosphorylation ratio by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, we prepared 136 peptide substrates. The correlation coefficient of phosphorylation ratios to CPM was 0.77 for all samples. However, the more the numbers of positively charged amino acids increased, the more the correlation coefficient increased. Although positively charged amino acids can have an effect on the correlation results, MALDI-TOF MS analysis is a useful means for monitoring phosphorylated peptide and protein kinase activity instead of radioactivity assays.

    DOI: 10.1016/j.ab.2011.08.035

  • Biodistribution and tumor localization of PEG-modified dendritic poly(L-lysine) oligonucleotide complexes

    R. Kurihara, D. Pissuwan, Takeshi Mori, Yoshiki Katayama, Takuro Niidome

    J. Biomater. Sci.-Polym. Ed.   23   2369 - 2380   2012年2月

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    記述言語:英語  

  • Transgene regulation system responding to Rho associated coiled-coil kinase (ROCK) activation. 査読 国際誌

    Akira Tsuchiya, Jeong-Hun Kang, Daisuke Asai, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

    Journal of controlled release : official journal of the Controlled Release Society   155 ( 1 )   40 - 6   2011年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Recently, we have proposed a new system of gene regulation called 'drug or gene delivery system responding to cellular signals' (D-RECS). In this system, transgene expression is activated in response to intracellular target protein kinases or proteases for safe, cell-specific gene delivery by using peptide-polymer conjugates. Here we applied this system to an intracellular Rho-associated coiled-coil kinase (ROCK) signal, which is activated abnormally in cardiovascular diseases. A ROCK responsive polymer consisting of neutral polymers in main chain and cationic ROCK substrate peptides in side chains was prepared and could form the complex with plasmid DNA. The complex was transferred into NIH3T3 cells with or without L-α-lysophosphatidic acid (LPA) that increases ROCK activity. At an N/P ratio of 2.0, a significant increase of the gene expression was identified in LPA-treated NIH3T3 cells, but was disappeared in NIH3T3 cells treated with ROCK specific inhibitor, Y-27632. These results suggest that the ROCK responsive polymer can regulate gene expression in response to ROCK activity.

    DOI: 10.1016/j.jconrel.2011.05.002

  • Effect of introduction of chondroitin sulfate into polymer-peptide conjugate responding to intracellular signals. 査読 国際誌

    Tetsuro Tomiyama, Riki Toita, Jeong-Hun Kang, Haruka Koga, Shujiro Shiosaki, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

    Nanoscale research letters   6 ( 1 )   532 - 532   2011年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We recently developed a novel tumor-targeted gene delivery system responding to hyperactivated intracellular signals. Polymeric carrier for gene delivery consists of hydrophilic neutral polymer as main chains and cationic peptide substrate for target enzyme as side chains, and was named polymer-peptide conjugate (PPC). Introduction of chondroitin sulfate (CS), which induces receptor-medicated endocytosis, into polymers mainly with a high cationic charge density such as polyethylenimine can increase tumor-targeted gene delivery. In the present study, we examined whether introduction of CS into PPC containing five cationic amino acids can increase gene expression in tumor cells. Size and zeta potential of plasmid DNA (pDNA)/PPC/CS complex were <200 nm and between -10 and -15 mV, respectively. In tumor cell experiments, pDNA/PPC/CS complex showed lower stability and gene regulation, compared with that of pDNA/PPC. Moreover, no difference in gene expression was identified between positive and negative polymer. These results were caused by fast disintegration of pDNA/PPC/CS complexes in the presence of serum. Thus, we suggest that introduction of negatively charged CS into polymers with a low charge density may lead to low stability and gene regulation of complexes.

    DOI: 10.1186/1556-276X-6-532

  • Fluorescent nanoparticles consisting of lipopeptides and fluorescein-modified polyanions for monitoring of protein kinase activity. 査読 国際誌

    Haruka Koga, Riki Toita, Takeshi Mori, Tetsuro Tomiyama, Jeong-Hun Kang, Takuro Niidome, Yoshiki Katayama

    Bioconjugate chemistry   22 ( 8 )   1526 - 34   2011年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Protein kinase (PK)-responsive nanoparticles (NPs) comprising a hydrophobically modified peptide substrate for PKs and a fluorescein-labeled polyanion (pA-F) were reported for monitoring PK activity via fluorescence intensity measurements. In this system, the formation of NPs by mixing lipopeptides and pA-Fs results in fluorescence quenching, while the quenched fluorescence recovered following dissociation of the NPs owing to the phosphorylation reaction of PKs. Eleven lipopeptides with different hydrophobic moieties (hydrocarbon and lithocholic acid) and four pA-Fs having main chains with differing flexibilities and fluorescein contents were synthesized and used to fabricate a series of twenty-four PK-responsive NP probes. The responses of the PK-responsive NP probes to PKs were evaluated to screen the most suitable NP probes. The assay system was then used to determine the IC(50) values for five inhibitors, the results of which were very similar to those previously reported. Thus, PK-responsive NPs are useful tools for high-throughput screening (HTS) of PK inhibitors.

    DOI: 10.1021/bc200066w

  • A hydrophilic polymer grafted with a histone tail peptide as an artificial gene regulator. 査読 国際誌

    Shujiro Shiosaki, Masanori Kuramoto, Riki Toita, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

    Bioorganic & medicinal chemistry   19 ( 13 )   4101 - 5   2011年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In chromatin, gene transcription is regulated through posttranslational modifications on the histone N-terminal tail sequences, typically an acetyl group modification on lysine residues. To realize a simple model of the gene regulation of chromatin, we designed a hydrophilic polymer grafted with histone H3 tail peptides. The polyplex formed from the polymer and DNA suppressed the gene expression effectively although the polyplex was weaker than the polyplex of poly-L-lysine and DNA. This weaker polyplex afforded the acetylation of the lysine residue of the grafted peptides by histone acetyltransferase. Subsequently, the gene expression was activated due to the relaxation of the polyplex which was brought by a cationic charge decrease in the grafted peptides. This molecular system is the first functional model of the gene regulation of the chromatin.

    DOI: 10.1016/j.bmc.2011.05.011

  • A simple set-and-mix assay for screening of protein kinase inhibitors in cell lysates, Anal. Biochem., 418, 44-49 (2011). 査読 国際誌

    Y. Asami, J. Oishi, H. Kitazaki, J. Kamimoto, J.-H. Kang, T. Niidome, T. Mori, Y. Katayama

    Anal Biochem   418   2011年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Controlled-release system of single-stranded DNA triggered by the photothermal effect of gold nanorods and its in vivo application

    S. Yamashita, H. Fukushima, Y. Akiyama, Y. Niidome, T. Mori, Y. Katayama, T. Niidome

    Bioorg. Med. Chem   19   2011年5月

  • Fluorescent Nanoparticles Consisting of Lipopeptides and Fluorescein-Modified Polyanions for Monitoring of Protein Kinase Activity

    H. Koga, R. Toita, T. Mori, T. Tomiyama, J.-H. Kang, T. Niidome, Y. Katayama

    Bioconjugate Chem   22   2011年5月

  • Transgene regulation system responding to Rho associated coiled-coil kinase (ROCK) activation, J. Control. Release, 155, 40-46 (2011).

    A. Tsuchiya, J.-H. Kang, D. Asai, T. Mori, T. Niidome, Y. Katayama

    J Cotrol Release   155   2011年5月

  • A hydrophilic polymer grafted with a histone tail peptide represents an artificial gene regulator activated by a histone acetyltransferase, Bioorg. Med. Chem., 19, 4101-41405 (2011).

    S. Shiosaki, M. Kuramoto, R. Toita, T. Mori, T. Niidome, Y. Katayama

    Bioorg Med Chem   19   2011年5月

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    記述言語:英語  

  • Optimization of peptide density on microarray surface for quantitative phosphoproteome, Anal. Sci., 27, 13-17 (2011). 査読 国際誌

    T. Shimomura, X. Han, A. Hata, T. Niidome, T. Mori, Y. Katayama,

    Anal Sci   2011年4月

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    掲載種別:研究論文(学術雑誌)  

  • Controlled-release system of single-stranded DNA triggered by the photothermal effect of gold nanorods and its in vivo application 査読 国際誌

    Shuji Yamashita, Hiromitsu Fukushima, Yasuyuki Akiyama, Yasuro Niidome, Takeshi Mori, Yoshiki Katayama, Takuro Niidome

    BIOORGANIC & MEDICINAL CHEMISTRY   19 ( 7 )   2130 - 2135   2011年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Gold nanorods have strong absorption bands in the near-infrared region, in which light penetrates deeply into tissues. The absorbed light energy is converted into heat by gold nanorods, the so-called 'photothermal effect'. Hence, gold nanorods are expected to act not only as on-demand thermal converters for photothermal therapy but also as controllers of a drug-release system responding to irradiation by near-infrared light. To achieve a controlled-release system that can be triggered by light irradiation, double-stranded DNA (dsDNA) was modified on gold nanorods. When the dsDNA-modified gold nanorods were irradiated by near-infrared light, the single-stranded DNA (ssDNA) was released from gold nanorods due to the photothermal effect. The amount of released ssDNA was dependent upon the power and exposure time of light irradiation. Release of ssDNA was also observed in tumors grown on mice after light irradiation. Such a controlled-release system of oligonucleotide triggered by the photothermal effect could expand the applications of gold nanorods that have unique optical characteristics in medicinal fields. (C) 2011 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.bmc.2011.02.042

  • Active Accumulation of Gold Nanorods in Tumor in Response to Near-Infrared Laser Irradiation

    A. Shiotani, Y. Akiyama, T. Kawano, Y. Niidome, T. Mori, Y. Katayama, T. Niidome

    Bioconjugate Chem.   21   2010年12月

  • Oligonuleotide delivery with dendritic poly(L-lysine) for treatments of the liver disorders

    K. Watanabe, M. Harada-Shiba, A. Suzuki, Y. Higuchi, S. Kawakami, M. Hashida, M. Hashida, R. Gokuden, R. Kurihara, Y. Sugao, T. Mori, Y. Katayama, Y. Katayama, T. Niidome, T. Niidome, T. Niidome

    Materials Research Society Symposium Proceedings   1237   12 - 17   2010年12月

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    記述言語:その他  

    A cationic peptide dendrier, dendritic poly(L-lysine), forms complexes with oligonucleotides and can deliver them to liver after intravenous injection. Here, we tried to deliver apolipoprotein B-specific siRNA for the treatment of hypercholesterolemia and NFκB decoy for the hepatitis treatment. Significant therapeutic effects in those disease model mice were observed after intravenous injection of the oligonucleotides complexes with dendritic poly(L-lysine). © 2010 Materials Research Society.

  • Tumor therapy by gene regulation system responding to cellular signal

    T. Tomiyama, T. Toita, J.-H. Kang, D. Asai, S. Shisaki, T. Mori, T. Niidome, Y. Katayama

    J. Control. Release   148   2010年11月

  • Tumor therapy by gene regulation system responding to cellular signal. 査読 国際誌

    Tetsuro Tomiyama, Riki Toita, Jeong-Hun Kang, Daisuke Asai, Shujiro Shiosaki, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

    Journal of controlled release : official journal of the Controlled Release Society   148 ( 1 )   101 - 105   2010年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    For safe and efficient gene therapy, the development of gene delivery systems to specifically target tumor cells is one of the most important issues regarding present gene delivery methodologies. Recently, we have developed a novel drug or gene delivery system responding to cellular signals (D-RECS) that can activate transgenes in response to hyperactivated cellular signals. Especially, a protein kinase C (PKC)α-responsive polymeric carrier (polymer-peptide conjugate, PPC(S)) showed highly specific gene expression to tumor cells and tissues. In the present study, we have applied the PKCα-responsive polymeric carrier to tumor gene therapy. PPC(S) consists of a polyacrylamide backbone and cationic peptide side chains, which together make PPC(S) as a positive polymer, a PKCα-specific substrate. A negative control polymer, PPC(A), was also prepared by replacing a serine residue at the phosphorylation site of the peptide side chains of PPC(S) with alanine. A complex of PPC(S) with caspase-8 or the herpes simplex virus-thymidine kinase (HSV-TK) gene as therapeutic genes was transfected into certain tumor cells or tissues. The prodrug ganciclovir (GCV) was then intraperitoneally injected into PPC(S)/HSV-TK complex-transfected mice. The PPC(S)/gene complex showed significant cytotoxicity toward the tumor cells and suppression of tumor growth, compared with those of the PPC(A)/gene complex or PBS. These results indicate that the PKCα-responsive polymeric carrier is applicable for tumor-targeted gene therapy.

    DOI: 10.1016/j.jconrel.2010.08.017

  • Targeted delivery of gold nanorods modified with thermo-sensitive polymer. 査読 国際誌

    Takuro Niidome, Atsushi Shiotani, Takeshi Mori, Yoshiki Katayama

    Journal of controlled release : official journal of the Controlled Release Society   148 ( 1 )   e65-6 - 6   2010年11月

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    記述言語:英語  

    DOI: 10.1016/j.jconrel.2010.07.027

  • Protein kinase substrate profiling with a high-density peptide microarray. 査読 国際誌

    Xiaoming Han, Tatsuhiko Sonoda, Takeshi Mori, Go Yamanouchi, Takayuki Yamaji, Syuhei Shigaki, Takuro Niidome, Yoshiki Katayama

    Combinatorial chemistry & high throughput screening   13 ( 9 )   777 - 89   2010年11月

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    記述言語:英語  

    We describe a powerful peptide microarray for profiling protein kinase substrates that combines the merits of chemoselective immobilization of peptides to achieve high density spots with the advantages of fluorescence-based analysis of phosphorylation for nonhazardous detection. For detection of on-chip phosphorylation, we used a fluorescence-labeled antiphosphotyrosine antibody to detect phosphotyrosine and a biotinylated Phostag, which was subsequently bound with a fluorescence-labeled streptavidin for phosphoserine/threonine. More than 290 kinds of Tyr peptides and over 1,100 kinds of Ser/Thr peptides were chemoselectively immobilized onto a glass surface in a high-density format to profile a panel of protein kinases, including c-Src, c-Abl, EGFR, JNK1, ERK2, p38α, and PKA. Many novel, highly reactive and specific peptides were identified as substrates for each protein kinase. Most substrates had the consensus motifs that have been reported previously but some new motifs were also found. The identification of two designed peptides that have higher reactivity than the famous PKA substrate (Kemptide) indicates that analysis of the amino acid biases of substrates is very helpful to the design of new substrates with high reactivity. Thus, the high-density peptide microarray is expected to be a powerful approach for high-throughput discovery of potential substrates for protein kinases.

  • Nanocomposite Formulation System of Lipid-Regulating Drugs Based on Layered Double Hydroxide: Synthesis, Characterization and Drug Release Properties 査読

    Mohamed R. Berber, Inas H. Hafez, Keiji Minagawa, Takeshi Mori, Masami Tanaka

    PHARMACEUTICAL RESEARCH   27 ( 11 )   2394 - 2401   2010年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    To design a nanocomposite formulation system of lipid-regulating drugs with versatile approaches using layered double hydroxide (LDH) material.
    The co-precipitation technique has been used to prepare the selected drugs [bezafibrate (BZF) and clofibric acid (CF)]-LDH nanocomposites. The nanocomposite materials (BZF-LDH and CF-LDH) were characterized by X-ray powder diffraction, infrared spectroscopy, thermogravimetric analysis, and scanning electron microscopy. The in vitro study was investigated in simulated gastrointestinal solutions at 36.8A degrees C.
    X-ray measurement and spectroscopic analysis indicated the formation of fully monophase drug-nanocomposites. The nanocomposites' gallery heights were calculated to be 23.5 and 16.3 for BZF and CF, respectively. The new gallery heights indicated that BZF and CF drugs have been stacked into LDH as a monolayer with a staggered inter-digitated arrangement. The size of the nanocomposites described by SEM microscopy was similar to aEuro parts per thousand 0.1 mu m. The nanocomposite formulation has improved the drugs properties (thermal stability, dissolution, and controlled release) beside the achievement of drug target delivery.
    Nanocomposites composed of lipid-regulating drugs (BZF and CF) with LDH were successfully synthesized as a new formulation system of this drug category. The LDH nanocomposite formulation system has improved the drugs release properties.

    DOI: 10.1007/s11095-010-0175-x

  • Active accumulation of gold nanorods in tumor in response to near-infrared laser irradiation. 査読 国際誌

    Atsushi Shiotani, Yasuyuki Akiyama, Takahito Kawano, Yasuro Niidome, Takeshi Mori, Yoshiki Katayama, Takuro Niidome

    Bioconjugate chemistry   21 ( 11 )   2049 - 54   2010年11月

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    記述言語:英語  

    Gold nanorods, rod-shaped gold nanoparticles, have strong absorbance in the near-infrared region, and the absorbed light energy can be converted to heat, the so-called photothermal effect. The gold nanorods were coated with thermoresponsive polymers, which have different phase transition temperatures that were controlled by adding comonomers, N,N-dimethylacrylamide (DMAA) or acrylamide (AAm) to N-isopropylacrylamide (NIPAM). The phase transition temperatures of poly(NIPAM-DMAA) and poly(NIPAM-AAm)-coated gold nanorods were 38 and 41 °C, respectively, while polyNIPAM-coated gold nanorods showed phase transition at 34 °C. Irradiation of the coated gold nanorods using the near-infrared laser induced a decrease in their sizes due to a phase transition of the polymer layers. Poly(NIPAM-AAm)-coated gold nanorods stably circulated in the blood flow without a phase transition after intravenous injection. Irradiation of near-infrared light at a tumor after the injection resulted in the gold specifically accumulating in the tumor. This novel accumulation technique which combines a thermoresponsive polymer and the photothermal effect of the gold nanorods should be a powerful tool for targeted delivery in response to light irradiation.

    DOI: 10.1021/bc100284s

  • Design of a Multifunctional Nanohybrid System of the Phytohormone Gibberellic Acid Using the Inorganic Layered Double Hydroxide Material

    H. Inas, M. R. Berber, K. Minagawa, T. Mori, M. Tanaka

    J. Agricult. Food Chem.   58   2010年10月

  • High-throughput detection of protein kinase activities in cell lysate based on the aggregation of gold nanoparticles with peptides

    Yoshiki Katayama, Yoshiki Katayama, Hirotaro Kitazaki, Jeong Hun Kang, Xiaoming Han, Takeshi Mori, Takeshi Mori, Takuro Niidome, Takuro Niidome

    Materials Research Society Symposium Proceedings   1241   39 - 44   2010年10月

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    記述言語:その他  

    Cationic peptides were used as coagulant of gold nano-particle (GNP, 20 nm) for the monitoring cellular protein kinase activities. If cationic peptides, which are designed as specific substrates to target protein kinases, were added into the GNP dispersion, the color of the dispersion changed from red to blue due to the aggregation. On the other hand, if the peptide was phosphorylated with target protein kinase, addition of the phosphorylated peptide didn&#039;t change the color of the GNP dispersion. Thus, this system can be a rapid assay of protein kinase activity. The method was applicable to the assay of intracellular protein kinase by using cell lysate. In addition, we applied the system for high-throughput screening of protein kinase inhibitor using 1300 kinds of chemical library. The result indicated that the method was also useful for the drug discovery. © 2010 Materials Research Society.

  • Protein kinase substrate profiling with a high-density peptide microarray

    X. Han, T. Sonoda, T. Mori, G. Yamanouchi, T. Yamajia, S. Shigakia, T. Niidome, Y. Katayama

    Comb. Chem. High Throughput Screening   13   2010年9月

  • Design of a Multifunctional Nanohybrid System of the Phytohormone Gibberellic Acid Using an Inorganic Layered Double-Hydroxide Material 査読

    Inas H. Hafez, Mohamed R. Berber, Keiji Minagawa, Takeshi Mori, Masami Tanaka

    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY   58 ( 18 )   10118 - 10123   2010年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    To offer a multifunctional and applicable system of the high-value biotechnological phytohormone gibberellic acid (GA), a nanohybrid system of GA using the inorganic Mg-Al layered double-hydroxide material (LDH) was formulated. The ion-exchange technique of LDH was applied to synthesize the GA-LDH hybrid. The hybrid structure of GA LDH was confirmed by different spectroscopic techniques. The nanohybrid size was described by SEM to be similar to 0.1 mu m. The GA-LDH nanohybrid structure was the key parameter that controlled GA properties. The layered molecular structure of LDH limited the interaction of GA molecules in two-dimensional directions. Accordingly, GA molecules did not crystallize and were released in an amorphous form suitable for dissolution. At various simulated soil solutions, the nanohybrids showed a sustained release process following Higuchi kinetics. The biodegradation process of the intercalated GA showed an extended period of soil preservation as well as a slow rate of degradation.

    DOI: 10.1021/jf102501n

  • Nanocomposite Formulation System of Lipid Regulating Drugs Based on Layered Double Hydroxide: Synthesis, Characterization and Drug Release Properties

    M. R. Berber, I. H. Hafez, K. Minagawa, T. Mori, M. Tanaka

    Pharm Res   27   2010年8月

  • Hepatoma-targeted gene delivery using a tumor cell-specific gene regulation system combined with a human liver cell-specific bionanocapsule. 査読 国際誌

    Jeong-Hun Kang, Jun Oishi, Jong-Hwan Kim, Moeko Ijuin, Riki Toita, Byungdug Jun, Daisuke Asai, Takeshi Mori, Takuro Niidome, Katsuyuki Tanizawa, Shun'ichi Kuroda, Yoshiki Katayama

    Nanomedicine : nanotechnology, biology, and medicine   6 ( 4 )   583 - 9   2010年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Hepatoma (hepatocellular carcinoma) is the most common type of malignant tumor originating in the liver and has a relatively low 5-year survival rate. The development of hepatoma-targeted therapy is needed to increase treatment efficiency and to reduce the incidence of undesirable side effects. In this study we developed a novel hepatoma-targeted gene delivery system. The gene delivery system was prepared by combining a human liver cell-specific bionanocapsule (BNC) and a tumor cell-specific gene regulation polymer, which responds to hyperactivated protein kinase C alpha in hepatoma cells. The complex of the polymer-DNA with BNCs was delivered into cells and tissues. The developed system showed increased transfection efficiency and resulted in cell-specific gene expression in hepatoma cells and tissues (HuH-7), but no gene expression in normal human hepatocytes or human epidermoid tumor cells (A431). The combination of a tumor cell-specific gene regulation system responding to protein kinase C alpha and BNCs showed excellent potential for the selective treatment of hepatomas. The system could be a useful method with applications in hepatoma-specific gene therapy and molecular imaging. From the clinical editor: Hepatocellular carcinoma is the most common type of malignant tumor in the liver with a low 5-year survival rate. In this study, a novel hepatoma-targeted gene delivery system was prepared by combining a human liver cell-specific bionanocapsule and a tumor cell-specific gene regulation polymer, which responds to hyperactivated protein kinase C (PKC)a in hepatoma cells. The system could be a useful in hepatoma-specific gene therapy and molecular imaging.

    DOI: 10.1016/j.nano.2010.01.007

  • A gene-delivery system specific for hepatoma cells and an intracellular kinase signal based on human liver-specific bionanocapsules and signal-responsive artificial polymer. 査読 国際誌

    Jun Oishi, Joohee Jung, Akira Tsuchiya, Riki Toita, Jeong-Hun Kang, Takeshi Mori, Takuro Niidome, Katsuyuki Tanizawa, Shun'ichi Kuroda, Yoshiki Katayama

    International journal of pharmaceutics   396 ( 1-2 )   174 - 8   2010年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Recently, our group has proposed a novel gene-regulation system responding to cAMP-dependent protein kinase (PKA) that has been applied to living cells. In this study, human liver-specific bionanocapsules (BNCs) are used as a gene-delivery system to increase transfection efficiency and to target specific cell types. BNCs can efficiently deliver a target gene to human hepatocytes and hepatoma cells in vitro or in vivo. The combination of a signal-responsive gene-delivery system with BNCs led to an increase in the transfection efficiency and selectivity for hepatoma cells. Expression from the delivered gene was identified from PKA-activated hepatoma cells (HepG2), but not from colon tumor cells (WiDr). These results show that the combination of a gene-regulation system responding to an intracellular signal with BNC can be used for the selective treatment of human hepatoma cells.

    DOI: 10.1016/j.ijpharm.2010.06.012

  • A gene delivery system specific for hepatoma cells and an intracellular kinase signal based on human liver-specific bionanocapsules and signal-responsive artificial polymer

    J. Oishi, J. Jung, A. Tsuchiya, R. Toita, J.-H. Kang; T. Mori, T. Niidome, K. Tanizawa, S. Kuroda, Y. Katayama

    Inter. J. Pharm.   396   2010年8月

  • Design of temperature-responsive α,α-disubstituted vinyl polymers with enhanced hysteresis

    T. Mori, S. Beppu, M. Berber, H. Mori, T. Makimura, A. Tsukamoto, K. Minagawa, T. Hirano, Masami Tanaka, T. Niidome, Y. Katayama, T. Hirano, Y. Maeda

    Langmuir   26   2010年6月

  • Unusually Large Hysteresis of Temperature-Responsive Poly(N-ethyl-2-propionamidoacrylamide) Studied by Microcalorimetry and FT-IR 査読

    Mohamed R. Berber, Hironori Mori, Inas H. Hafez, Keiji Minagawa, Masami Tanaka, Takuro Niidome, Yoshiki Katayama, Atsushi Maruyama, Tomohiro Hirano, Yasushi Maeda, Takeshi Mori

    JOURNAL OF PHYSICAL CHEMISTRY B   114 ( 23 )   7784 - 7790   2010年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We reported here the full characterization of the hysteresis of the phase transition behavior of an aqueous solution of poly(N-ethyl-2-propionamidoacrylamide) (PNEPA), which has a unique alpha,alpha-disubstituted structure, by using microcalorimetry and FT-IR. Phase transition temperatures near the thermodynamic equilibrium were determined by extrapolating the scanning rate of the microcalorimetry to zero. The calculated hysteresis from the phase transition temperature was unusually very large (similar to 8 degrees C). FT-IR analysis indicated that the large hysteresis of PNEPA resulted from a coupling of intra-/intermonomeric unit hydrogen bonds, which is known to occur in a beta-sheet of proteins but has never been reported in temperature-responsive polymers. The effects of the molecular weight and polymer concentration on the hysteresis were studied by using fractionated PNEPAs and it was found that a low molecular weight and a low concentration enhanced the hysteresis.

    DOI: 10.1021/jp102681q

  • Design of temperature-responsive polymers with enhanced hysteresis: alpha,alpha-disubstituted vinyl polymers. 査読 国際誌

    Takeshi Mori, Suguru Beppu, Mohamed R Berber, Hironori Mori, Takumi Makimura, Ayako Tsukamoto, Keiji Minagawa, Tomohiro Hirano, Masami Tanaka, Takuro Niidome, Yoshiki Katayama, Tatsuya Hirano, Yasushi Maeda

    Langmuir : the ACS journal of surfaces and colloids   26 ( 12 )   9224 - 32   2010年6月

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    記述言語:英語  

    Three temperature-responsive polymers which are alpha,alpha-disubstituted vinyl polymers having two amphiphilic groups (ethylamide or ethylester) per monomeric unit were designed. Two of these polymers showed unusually large hysteresis in their phase transition temperatures between a heating and a cooling process. This hysteresis resulted from the extremely slow kinetics of the dissolution process of the aggregated polymer chains in the cooling process due to intra- and interchain interactions including hydrogen bonding and hydrophobic interaction. The high density of the amphiphilic substituents on the polymer chain due to the alpha,alpha-disubstituted structure enhanced these intra- and interchain interactions. The large hysteresis was also observed in the volume change of a corresponding hydrogel. These new classes of temperature-responsive polymers are interesting materials because their large hystereses can be regarded as erasable memory function.

    DOI: 10.1021/la100020t

  • Controlled release of PEG chain from gold nanorods: targeted delivery to tumor. 査読 国際誌

    Takuro Niidome, Akira Ohga, Yasuyuki Akiyama, Kazuto Watanabe, Yasuro Niidome, Takeshi Mori, Yoshiki Katayama

    Bioorganic & medicinal chemistry   18 ( 12 )   4453 - 8   2010年6月

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    記述言語:英語  

    Gold nanorods exhibit strong absorbance of light in the near infrared region, which penetrates deeply into tissues. Since the absorbed light energy is converted into heat, gold nanorods are expected to act as a contrast agent for in vivo bioimaging and as a thermal converter for photothermal therapy. To construct a gold nanorod targeted delivery system for tumor a peptide substrate for urokinase-type plasminogen activator (uPA), expressed specifically on malignant tumors, was inserted between the PEG chain and the surface of the gold nanorods. In other words, we constructed PEG-peptide-modified gold nanorods. After mixing the gold nanorods with uPA, the PEG chain was released from the surface of the gold and subsequently nanorod aggregation took place. The formation of the aggregation was monitored as a decrease in light absorption at 900 nm. Tumor homogenate induced a significant decrease in this absorption. Larger amount of the PEG-peptide-modified gold nanorods bound to cells expressing uPA in vitro compared with control gold nanorods, which had scrambled sequence of the peptide. The PEG-peptide-modified gold nanorods showed higher accumulation in tumor than the control after they were injected intravenously into tumor-bearing mice, however, the density of the peptide on the surface of the gold nanorods was a key factor of their biodistributions. This targeted delivery system, which responds to uPA activity, is expected to be a powerful tool for tumor bioimaging and photothermal tumor therapy.

    DOI: 10.1016/j.bmc.2010.04.070

  • Unusually Large Hysteresis of Temperature-Responsive Poly(N-ethyl-2-propionamidoacrylamide) Studied by Microcalorimetry and FT-IR

    M. R. Berber, H. Mori, I. H. Hafez, K. Minagawa, M. Tanaka, T. Niidome, Y. Katayama, A. Maruyama, T. Hirano, Y. Maeda, T. Mori

    J. Phys. Chem. B   2010年6月

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    DOI: 114

  • Hepatoma-targeted gene delivery using a tumor cell-specific gene regulation system combined with a human liver cell-specific bionanocapsule

    J.-H. Kang, J. Oishi, J.-H. Kim, M. Ijuin, R. Toita, B. Jun, D. Asai, T. Mori, T. Niidome, K. Tanizawa, S. Kuroda, Y. Katayama

    Nanomedicine: NBM   6   2010年5月

  • Controlled release of PEG chain from gold nanorods: targeted delivery to tumor

    T. Niidome, A. Ohga, Y. Akiyama, K. Watanabe, Y. Niidome, T. Mori, Y. Katayama

    Bioorg. Med. Chem   18   2010年5月

  • Polydispersity as a parameter for indicating the thermal stability of proteins by dynamic light scattering

    K. Shiba, T. Niidome, E. Katoh, H. X., L. Han, T. Mori, Y. Katayama

    Anal Sci   26   2010年5月

  • Gold nanoparticle-based colorimetric assay for cancer diagnosis. 査読 国際誌

    Jeong-Hun Kang, Yoji Asami, Masaharu Murata, Hirotaro Kitazaki, Noriaki Sadanaga, Eriko Tokunaga, Satoko Shiotani, Satoko Okada, Yoshihiko Maehara, Takuro Niidome, Makoto Hashizume, Takeshi Mori, Yoshiki Katayama

    Biosensors & bioelectronics   25 ( 8 )   1869 - 74   2010年4月

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    記述言語:英語  

    A novel gold nanoparticle (GNP)-based colorimetric assay was developed for cancer diagnosis. This system is based on the noncrosslinking aggregation mechanism with a cationic protein kinase C (PKC) alpha-specific peptide substrate, which is used as a coagulant of citrate-coated GNP with anionic surface charges. The phosphorylation of peptide substrates by PKCalpha suppressed GNP aggregation, resulting in a red color, but in the case of non-phosphorylation, the color of the GNP solution changed from red to blue, indicating particle aggregation. Moreover, a correlation between the color change of the GNP dispersions and the level of activated PKCalpha was identified from experiments using cancer cell lines, or xenografted mouse cancer and normal mouse tissues. When our system was applied to human breast cancers and normal human breast tissues, cancer tissue lysates became red in color, indicating GNP dispersion, while all lysates from normal tissue turned the GNP solution blue. MALDI-TOF MS analysis and Western blotting experiment confirmed that these different results between cancer and normal tissues reflected the difference in PKCalpha activity. This study is the first report on the application of the GNP-based colorimetric assay to the diagnosis of cancer.

    DOI: 10.1016/j.bios.2009.12.022

  • Specific transgene expression in HIV-infected cells using protease-cleavable transcription regulator

    D. Asai, M. Kuramoto, Y. Shoji, K. B. Kodama, J.-H. Kang, K. Kawamura, H. Miyoshi, T. Mori, T. Niidome, H. Nakashima, Y. Katayama

    J. Control. Release   141   2010年2月

  • Gold nanoparticle-based colorimetric assay for cancer diagnosis

    J.-H. Kang, Y. Asami, M. Murata, H. Kitazaki, N. Sadanaga, E. Tokunaga, S. Shiotani, S. Okada, Y. Maehara, T. Niidome, M. Hashizume, T. Mori, Y. Katayama

    Biosens. Bioelectron.   25   2010年2月

  • Specific transgene expression in HIV-infected cells using protease-cleavable transcription regulator. 査読 国際誌

    Daisuke Asai, Masanori Kuramoto, Yoko Shoji, Jeong-Hun Kang, Kota Bae Kodama, Kenji Kawamura, Takeshi Mori, Hiroshi Miyoshi, Takuro Niidome, Hideki Nakashima, Yoshiki Katayama

    Journal of controlled release : official journal of the Controlled Release Society   141 ( 1 )   52 - 61   2010年1月

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    記述言語:英語  

    Gene therapy is a promising strategy for the treatment of HIV infection, but cell specificity remains an issue. Recently we have developed a new concept for a drug or gene delivery system responding to cellular signals (D-RECS) to achieve cell-specific transgene expression using a non-viral polymer-based vehicle. According to this concept, intracellular signaling enzymes, which are activated specifically in target cells, are used to trigger transgene expression. We previously applied this concept to HIV-1 protease and showed that the recombinant protease could act as a suitable signal. Here we further developed this system to achieve highly specific transgene expression in HIV-infected cells. We prepared a polymeric gene regulator grafted with a cationic peptide containing the HIV-Tat peptide via a specific substrate for HIV-1 protease. The regulator formed a stable polyplex with the transgene, suppressing its transcription. HIV-1 protease cleaved the peptide and released the transgene, which was consequently expressed specifically in activated HIV-infected cells, but remained unreleased and inactive in uninfected cells. The validity of this approach was further confirmed by applying it to the CVB1 2A protease of coxsackievirus (Picornaviridae family). This strategy should be widely applicable for specific expression of a variety of therapeutic genes in virus-infected cells.

    DOI: 10.1016/j.jconrel.2009.08.025

  • The effects of PEG grafting level and injection dose on gold nanorod biodistribution in tumor-bearing mice

    Y. Akiyama, T. Mori, Y. Katayama, T. Niidome

    J. Control. Release   139   2009年12月

  • DECOY DELIVERY INTO LIVER USING DENDRITIC POLY(L-LYSINE) 査読

    Takuro Niidome, Yusuke Sugao, Kazuto Watanabe, Takeshi Mori, Yoshiki Katayama

    JOURNAL OF GENE MEDICINE   11 ( 12 )   1165 - 1165   2009年12月

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    記述言語:英語  

  • Protein kinase Cα-specific peptide substrate graft-type copolymer for cancer cell-specific gene regulation systems

    R. Toita, J.-H. Kang, J.-H. Kim, T. Tomiyama, T. Mori, T. Niidome, B. Jun, Y. Katayama

    J. Control. Release   139   2009年11月

  • Cellular signal-specific peptide substrate is essential for the gene delivery system responding to cellular signals. 査読 国際誌

    Jeong-Hun Kang, Riki Toita, Tetsuro Tomiyama, Jun Oishi, Daisuke Asai, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

    Bioorganic & medicinal chemistry letters   19 ( 21 )   6082 - 6   2009年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Recently, there is a growing interest in the intracellular signal-targeting gene therapy or diagnosis, mainly by using the reaction of targeting enzymes with peptide substrates. In the present study, we proved the importance of target intracellular signal-specificity peptide substrate for intracellular signals-targeting gene therapy or diagnosis. Protein kinase C (PKC) was used as a trigger to activate the transgene expression. Two peptides, a positive peptide showing phosphorylation levels on several PKC isozymes (PKCalpha, betaII, gamma, epsilon, eta, zeta, and iota/lambda) and a negative peptide in which the phosphorylation site was destroyed by changing from serine to alanine, were designed. Moreover, two polymers possessing each peptide as a pendant chain, a PKC-responsive conjugate [PPC(S)] and a negative control conjugate [PPC(A)], were synthesized. After the introduction of complexes into cells or tissues, gene expression for PPC(S)/DNA complexes was higher that for PPC(A)/DNA complexes. However, no difference in gene expression between B16 melanoma tumors and normal skin tissues was identified. These results suggest that a peptide substrate specific to a target intracellular signal is very important for intracellular signals-targeting gene therapy or diagnosis.

    DOI: 10.1016/j.bmcl.2009.09.034

  • Cellular signal-specific peptide substrate is essential for the gene delivery system responding to cellular signals 国際誌

    J.-H. Kang, R. Toita, T. Tomiyama, J. Oishi, D. Asai, T. Mori, T. Niidome, Y. Katayama

    Bioorg. Med. Chem. Lett.   2009年10月

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    掲載種別:研究論文(学術雑誌)  

  • The effects of PEG grafting level and injection dose on gold nanorod biodistribution in the tumor-bearing mice. 査読 国際誌

    Yasuyuki Akiyama, Takeshi Mori, Yoshiki Katayama, Takuro Niidome

    Journal of controlled release : official journal of the Controlled Release Society   139 ( 1 )   81 - 4   2009年10月

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    記述言語:英語  

    Gold nanorods have strong absorbance in the near infrared region, which penetrates deeply into tissues, where the absorbed light energy is converted into heat. Therefore, gold nanorods are expected to act as an effective contrast agent for in vivo bioimaging and as a thermal converter for photothermal therapy. We grafted various amounts of polyethylene glycol (PEG) onto the surface of gold nanorods and investigated the effects of grafting level and injection dose on the biodistribution in the tumor-bearing mice after intravenous injection and enhanced permeability and retention (EPR). Higher PEG grafting levels were advantageous for reticuloendothelial system (RES) avoidance and for suppression of aggregation of the gold nanorods in the circulation. Modification with a PEG:gold molar ratio of 1.5 was sufficient to show both prolonged circulation and the EPR effect. When the injection dose was increased above 19.5 microg of gold, the RES uptake in the liver was saturated and surplus gold nanorods were distributed to other tissues, especially the spleen and the tumor. The results of this study will provide an important basis for the development of cancer therapies mediated by the photothermal effect of gold nanorods.

    DOI: 10.1016/j.jconrel.2009.06.006

  • Protein kinase C alpha-specific peptide substrate graft-type copolymer for cancer cell-specific gene regulation systems. 査読 国際誌

    Riki Toita, Jeong-Hun Kang, Jong-Hwan Kim, Tetsuro Tomiyama, Takeshi Mori, Takuro Niidome, Byungdug Jun, Yoshiki Katayama

    Journal of controlled release : official journal of the Controlled Release Society   139 ( 2 )   133 - 9   2009年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We recently proposed a novel gene regulation system responding to specifically and abnormally activated intracellular enzymes in diseased cells. In the present study, we focused on protein kinase C (PKC)alpha, which is hyper-activated in most tumor cells, as a trigger for transgene regulation. We prepared cationic copolymers comprising hydrophilic and neutral polymers in main chains and cationic peptide substrates with different contents in side chains. Our copolymer with high peptide content (>3 mol%) condensed with pDNA more weakly than with poly(L-lysine) (pLL) having a similar molecular weight, but gene suppression was nearly identical to that of pLL, probably due to the steric hindrance of the main chains in our copolymer. Steric hindrance of the main chains barely affected the phosphorylation reaction of the pendant peptide. In cell and mouse experiments, higher gene expression was observed in complexes of pDNA with copolymers pended PKC alpha-specific substrate peptide than that in complexes with negative copolymers pended peptide substituted phosphorylation site of serine residues with alanine. These results indicate that our system can recognize intracellular PKC alpha as a trigger to regulate transgene expression, and may be useful for tumor gene therapy.

    DOI: 10.1016/j.jconrel.2009.06.011

  • Establishment of screening system toward discovery of kinase inhibitors using label-free on-chip phosphorylation assays

    K. Inamori, M. Kyo, K. Matsukawa, Y. Inoue, T. Sonoda, T. Mori, T. Niidome, Y. Katayama

    BioSystems   97   2009年10月

  • PNIPAM gel-coated gold nanorods for targeted delivery responding to a near-infrared laser

    T. Kawano, Y Niidome, T. Mori, Y. Katayama, T. Niidome

    Bioconjugate Chem.   20   2009年10月

  • A novel protease activity assay using a protease-responsive chaperone protein

    K. Sao, M. Murata, Y. Fujisaki, K. Umezaki, T. Mori, T. Niidome, Y. Katayama, M. Hashizume

    Biochem. Biophys. Res. Commun.   383   2009年9月

  • Establishment of screening system toward discovery of kinase inhibitors using label-free on-chip phosphorylation assays. 査読 国際誌

    Kazuki Inamori, Motoki Kyo, Kazuki Matsukawa, Yusuke Inoue, Tatsuhiko Sonoda, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

    Bio Systems   97 ( 3 )   179 - 85   2009年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We describe a label-free method for the kinase inhibition assay toward discovery of kinase inhibitors. The surface plasmon resonance (SPR) imaging analysis using zinc(II) compound was adopted on the on-chip phosphorylation analysis. In this study, following three subjects were focused: (1) to monitor the inhibition of three inhibitors supporting by their specific inhibition mechanisms, (2) to quantify the inhibitory activities, and (3) to prove the reliability of the obtained 50% inhibition concentration (IC(50)) value. First, the inhibitory activities of Amide 5-24, H-89 and Gö6983 on PKA and PKCdelta were determined, and specific inhibitions for two kinases could be observed quantitatively. Second, the inhibition curves of Amide 5-24, Amide 14-22 and H-89 were obtained, and the results supported the two previous reports: (1) the inhibition efficiency of Amide 5-24 was much higher than that of Amide 14-22, and (2) the inhibitory activity of H-89 followed ATP-binding site blocking mechanism. Last, the obtained IC(50) values by the SPR imaging were almost corresponded to those by the solution assay, although on-chip phosphorylation efficiency was low (approximately 12%). In conclusion, validation of the on-chip phosphorylation analysis for kinase inhibitors was achieved. This label-free method might be applied for discovery of kinase inhibitors.

    DOI: 10.1016/j.biosystems.2009.04.007

  • Heterotactic-Specific Radical Polymerization of N-Isopropylacrylamide and Phase Transition Behavior of Aqueous Solution of Heterotactic Poly(N-isopropylacrylamide)

    T. Hirano, T. Kamikubo, Y. Okumura, Y. Bando, R. Yamaoka, T. Mori, K. Ute

    J. Polym. Sci., Part A: Polym. Chem. Ed.   47   2009年9月

  • Poly(ethylene glycol)-modified gold nanorods as a photothermal nanodevice for hyperthermia

    T. Niidome, Y. Akiyama, M. Yamagata, T. Kawano, T. Mori, Y. Niidome, Y. Katayama

    J. Biomater. Sci.-Polym. Ed.   20   2009年8月

  • Inflammatory cell-specific transgene expression system responding to Ikappa-B kinase beta activation. 査読 国際誌

    Daisuke Asai, Akira Tsuchiya, Jeong-Hun Kang, Kenji Kawamura, Jun Oishi, Takeshi Mori, Takuro Niidome, Yoko Shoji, Hideki Nakashima, Yoshiki Katayama

    The journal of gene medicine   11 ( 7 )   624 - 32   2009年7月

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    記述言語:英語  

    BACKGROUND: Control of inflammation is essential for the clinical management of many common human diseases. However, there are few generally applicable strategies to convert an abnormal intracellular signal into a gene expression that leads to normalization of the intracellular environment. Recently, we proposed a novel strategy termed D-RECS (i.e. drug or gene delivery system responding to cellular signals) to convert an intracellular signal to transgene expression. In the present study, we applied this concept to inflammatory cells using Ikappa-B kinase as a signal molecule that triggers the gene expression. METHODS: Candidate cationic substrates of Ikappa-B kinase (IKK)beta were synthesized and their reactivity was investigated. Then, polymers grafted with these peptides were prepared by radical polymerization. Polymer/DNA complexes (polyplexes) were prepared by mixing plasmid DNAs with the polymers. The behaviour of these polyplexes by adding IKKbeta was examined. Furthermore, changes of gene expression were evaluated after the microinjection of polyplex into living cells under conditions of nuclear factor (NF)-kappaB activation. RESULTS: Synthetic peptides with additional lysine residues were well phosphorylated by IKKbeta. Both the polymer and the polyplex were also phosphorylated by IKKbeta. The results of gel shift assay showed that the polyplex was disintegrated and free DNA was released in the presence of IKKbeta. The polyplex comprising-green fluorescent protein plasmid DNA and the polymer expressed the transgene in living cells exposed to a pro-inflammatory stimulus. CONCLUSIONS: Our concept of cell-specific gene expression was demonstrated to work in inflammatory cells. This method may provide a unique strategy for gene therapy exclusively in inflammatory cells.

    DOI: 10.1002/jgm.1342

  • In vivo siRNA delivery with dendritic poly(L-lysine) for the treatment of hypercholesterolemia

    K. Watanabe, M. Harada-Shiba, A. Suzuki, R. Gokuden, R. Kurihara, Y. Sugao, T. Mori, Y. Katayama, T. Niidome

    Molecular BioSystems   5   2009年6月

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    記述言語:英語  

  • A novel protease activity assay using a protease-responsive chaperone protein. 査読 国際誌

    Kentaro Sao, Masaharu Murata, Yuri Fujisaki, Kaori Umezaki, Takeshi Mori, Takuro Niidome, Yoshiki Katayama, Makoto Hashizume

    Biochemical and biophysical research communications   383 ( 3 )   293 - 7   2009年6月

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    記述言語:英語  

    Protease activity assays are important for elucidating protease function and for developing new therapeutic agents. In this study, a novel turbidimetric method for determining the protease activity using a protease-responsive chaperone protein is described. For this purpose, a recombinant small heat-shock protein (sHSP) with an introduced Factor Xa protease recognition site was synthesized in bacteria. This recombinant mutant, FXa-HSP, exhibited chaperone-like activity at high temperatures in cell lysates. However, the chaperone-like activity of FXa-HSP decreased dramatically following treatment with Factor Xa. Protein precipitation was subsequently observed in the cell lysates. The reaction was Factor Xa concentration-dependent and was quantitatively suppressed by a specific inhibitor for Factor Xa. Protein aggregation was detected by a simple method based on turbidimetry. The results clearly demonstrate that this assay is an effective, easy-to-use method for determining protease activities without the requirement of labeling procedures and the use of radioisotopes.

    DOI: 10.1016/j.bbrc.2009.03.129

  • In vivo siRNA delivery with dendritic poly(L-lysine) for the treatment of hypercholesterolemia

    K. Watanabe, M. Harada-Shiba, A. Suzuki, R. Gokuden, R. Kurihara, Y. Sugao, T. Mori, Y. Katayama, T. Niidome

    Molecular BioSystems   5   2009年6月

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    記述言語:英語  

  • In vivo siRNA delivery with dendritic poly(L-lysine) for the treatment of hypercholesterolemia

    K. Watanabe, M. Harada-Shiba, A. Suzuki, R. Gokuden, R. Kurihara, Y. Sugao, T. Mori, Y. Katayama, T. Niidome

    Molecular BioSystems   5   2009年6月

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    記述言語:英語  

  • Molecular mechanism of Caspase-3-induced gene expression of polyplexes formed from polycations grafted with cationic substrate peptides 国際誌

    K. Kawamura, M. Kuramoto, T. Mori, R. Toita, J. Oishi, Y. Sato, J.-H. Kang, D. Asai, T. Niidome, Y. Katayama

    J. Biomater. Sci.: Polym. Ed.   2009年5月

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    掲載種別:研究論文(学術雑誌)  

  • Signal-to-noise ratio improvement of peptide microarrays by using hyperbranched-polymer materials

    Takeshi Mori, Go Yamanouchi, Xiaoming Han, Yusuke Inoue, Syuhei Shigaki, Takayuki Yamaji, Tatsuhiko Sonoda, Kei Yasui, Hisato Hayashi, Takuro Niidome, Yoshiki Katayama

    JOURNAL OF APPLIED PHYSICS   105 ( 10 )   102020   2009年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The fabrication of peptide microarrays using hyperbranched polymers (HBPs) to improve the signal-to-noise ratio was demonstrated. Due to a high density of reactive groups at the chain ends of the HBPs, as well as to their spherical shape, HBPs can be used as linkers to increase the amount of immobilized peptides through raising the specific surface area of the glass substrate. A zwitterionic HBP was used as a blocking agent to reduce the noise level of the peptide microarrays. The zwitterionic HBP shows comparably excellent blocking ability to a commercially available BSA-based blocking agent. Thus, it was concluded that HBPs have high potential for the fabrication of highly sensitive peptide microarrays.

    DOI: 10.1063/1.3116124

  • Heterotactic-Specific Radical Polymerization of N-isopropylacrylamide and Phase Transition Behavior of Aqueous Solution of Heterotactic Poly(N-isopropylacrylamide) 査読

    Tomohiro Hirano, Takahiro Kamikubo, Yuya Okumura, Yoichi Bando, Ryosuke Yamaoka, Takeshi Mori, Koichi Ute

    JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY   47 ( 10 )   2539 - 2550   2009年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Radical polymerization of N-isopropylacrylamide (NIPAAm) in toluene at low temperatures, in the presence of fluorinated-alcohols, produced heterotactic polymer comprising an alternating sequence of meso and racemo dyads. The heterotacticity reached 70% in triads when polymerization was carried out at -40 degrees C using nonafluoro-tert-butanol as the added alcohol. NMR analysis revealed that formation of a 1:1 complex of NIPAAm and fluorinated-alcohol through C=O center dot center dot center dot H-O hydrogen bonding induces the heterotactic specificity. A mechanism for the heterotactic-specific polymerization is proposed. Examination of the phase transition behavior of aqueous solutions of heterotactic poly(NIPAAm) revealed that the hysteresis of the phase transition between the heating and cooling cycles depended on the average length of meso dyads in poly(NIPAAm). (C) 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: 2539-2550, 2009

    DOI: 10.1002/pola.23338

  • Signal-to-noise ratio improvement of peptide microarrays by using hyperbranched-polymer materials 国際誌

    T. Mori, G. Yamanouchi, X. Han, Y. Inoue, S. Shigaki, T. Yamaji, T. Sonoda, K. Yasui, H. Hayashi, T. Niidome, Y. Katayama

    J. Appl. Phys   2009年4月

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    掲載種別:研究論文(学術雑誌)  

  • Molecular design of protein-based nanocapsules for stimulus-responsive characteristics 国際誌

    K. Sao, M. Murata, K. Umezaki, Y. Fujisaki, T. Mori, T. Niidome, Y. Katayama, M. Hashizume

    Bioorg. Med. Chem.   2009年4月

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    掲載種別:研究論文(学術雑誌)  

  • Inflammatory cell-specific gene regulation system responding to Ikappa-B kinase beta activation

    D. Asai, A. Tsuchiya, J.-H. Kang, K. Kawamura, J. Oishi, T. Mori, T. Niidome, Y. Shoji, H. Nakashima, Y. Katayama

    J. Gene Med.   11   2009年3月

  • Monitoring protein kinase activity in cell lysates using a high-density peptide microarray. 査読 国際誌

    Xiaoming Han, Go Yamanouchi, Takeshi Mori, Jeong-Hun Kang, Takuro Niidome, Yoshiki Katayama

    Journal of biomolecular screening   14 ( 3 )   256 - 62   2009年3月

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    記述言語:英語  

    Monitoring and targeting protein kinases is widely accepted as a promising approach for disease diagnosis and drug discovery. For this purpose, the authors have developed an original type of peptide array as a high-throughput screening assay for quantitatively evaluating kinase activity. A volume of 2 nL of peptide solution was spotted onto a formyl group-modified glass slide by using an arrayer, which was designed for use with protein chip technology. The phosphorylation was recognized by fluorescence-label antibody and detected with an automatic microarray scanner widely used in DNA chip technology. The system needs low sample volume, provides a high-density peptide array, and supplies high reproducibility. It provided enough sensitivity for inhibitor screening, even though a relatively low concentration of purified kinase was employed. The assay also proved useful for the detection of intracellular kinase activity as well as for the measurement of the fluctuations of intracellular protein kinase activity with drug stimulation. Thus, this peptide array would be applicable for kinase-targeted diagnosis, cell-based drug screening, and signal pathway investigation.

    DOI: 10.1177/1087057108329348

  • PNIPAM gel-coated gold nanorods for targeted delivery responding to a near-infrared laser

    T. Kawano, Y Niidome, T. Mori, Y. Katayama, T. Niidome

    Bioconjugate Chem.   2009年2月

  • PNIPAM gel-coated gold nanorods for targeted delivery responding to a near-infrared laser. 査読 国際誌

    Takahito Kawano, Yasuro Niidome, Takeshi Mori, Yoshiki Katayama, Takuro Niidome

    Bioconjugate chemistry   20 ( 2 )   209 - 12   2009年2月

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    記述言語:英語  

    Gold nanorods can be used as photothermal converters, permitting near-infrared (NIR) light to be transmitted deep into tissues without causing damage. We prepared hybrid nanorods with a core-shell structure, i.e., a single gold nanorod encapsulated in a poly (N-isopropylacrylamide) nanogel. Hybrid nanorods demonstrated remote, reversible, pulsatile phase transition and in vivo action after irradiation using a NIR laser.

    DOI: 10.1021/bc800480k

  • A novel protease activity assay using a protease-responsive chaperone protein 国際誌

    K. Sao, M. Murata, Y. Fujisaki, K. Umezaki, T. Mori, T. Niidome, Y. Katayama, M. Hashizume

    Biochem. Biophys. Res. Commun.   2009年1月

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    掲載種別:研究論文(学術雑誌)  

  • Syndiotactic poly(N-n-propylacrylamide) shows highly cooperative phase transition. 査読 国際誌

    Takeshi Mori, Tomohiro Hirano, Atsushi Maruyama, Yoshiki Katayama, Takuro Niidome, Yoichi Bando, Koichi Ute, Shinji Takaku, Yasushi Maeda

    Langmuir : the ACS journal of surfaces and colloids   25 ( 1 )   48 - 50   2009年1月

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    記述言語:英語  

    Syndiotactic poly(N-n-propylacrylamide)s (PNNPAM) with various racemo (r) diad contents were synthesized, and their phase transition behaviors were studied by high-sensitivity differential scanning calorimetry and FT-IR spectroscopy. The cooperativity of the phase transition increased with the increase in the r diad content. Compared with the atactic PNNPAM, the number of cooperative units, which shows the monomeric units length undergoing cooperative phase transition, increased 2.5- to 4-fold in the syndiotactic polymers. From the results of FT-IR spectroscopy and quantum chemical calculations, it was determined that the high cooperativity of the syndiotactic polymers resulted from local formation of an ordered structure in a dehydrated state.

    DOI: 10.1021/la8034837

  • Molecular design of protein-based nanocapsules for stimulus-responsive characteristics. 査読 国際誌

    Kentaro Sao, Masaharu Murata, Kaori Umezaki, Yuri Fujisaki, Takeshi Mori, Takuro Niidome, Yoshiki Katayama, Makoto Hashizume

    Bioorganic & medicinal chemistry   17 ( 1 )   85 - 93   2009年1月

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    記述言語:英語  

    Hsp16.5, a small heat-shock protein (sHSP) from hyperthermophilic archaeon, forms a homogeneous complex comprised of 24 subunits with a molecular mass of 400 kDa. This complex self-organizes under physiological conditions, and the structure of the complex is a nanoscale spherical capsule with small pores. Furthermore, this natural nanocapsule exhibits very high thermal stability. In this paper, we functionalized the nanocapsule to control the structure in response to external stimuli such as a protease signal and temperature. For this purpose, several mutations (Mut1-10) to create a cleavage site for a specific protease, Factor Xa, were introduced on the outer surface of the nanocapsule using a genetic engineering strategy. The resulting mutants were expressed to high levels in Escherichia coli. One of these mutants, Mut6, which has the most accessible cleavage site located at the triangular pore on the surface of the capsule, formed a spherical assembly similar to that observed for the wild-type protein. Mut6 showed the highest sensitivity to Factor Xa, and the structure of the protease digested Mut6 disassembled irreversibly after heating. In contrast, the nanocapsule comprising the wild-type Hsp16.5 was not influenced by the dual stimuli. These results suggest that Mut6 acts as a stimulus-responsive nanocapsule. Such a characteristic of the protein-based nanocapsule has attractive potential as a versatile intelligent system.

    DOI: 10.1016/j.bmc.2008.11.013

  • Design of polymeric carriers for cancer-specific gene targeting: utilization of abnormal protein kinase Calpha activation in cancer cells. 査読 国際誌

    Jeong-Hun Kang, Daisuke Asai, Jong-Hwan Kim, Takeshi Mori, Riki Toita, Tetsuro Tomiyama, Yoji Asami, Jun Oishi, Yuko T Sato, Takuro Niidome, Byungdug Jun, Hideki Nakashima, Yoshiki Katayama

    Journal of the American Chemical Society   130 ( 45 )   14906 - 7   2008年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We succeeded in cancer cell specific gene expression by using a polyplex responsive to protein kinase Calpha, which is activated in various types of cancer cells.

    DOI: 10.1021/ja805364s

  • Nanocomposites of 2-arylpropionic acid drugs based on Mg-Al layered double hydroxide for dissolution enhancement. 査読 国際誌

    Mohamed R Berber, Keiji Minagawa, Masahiro Katoh, Takeshi Mori, Masami Tanaka

    European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences   35 ( 4 )   354 - 60   2008年11月

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    記述言語:英語  

    Naproxen (NP) and flurpibrofen (FB) as non-steroidal anti-inflammatory drugs (NSAIDs) of 2-arylpropionic acid derivatives have been used as host organic drugs to be intercalated into layered double hydroxide (LDH) applying reconstruction and co-precipitation techniques. The obtained NP-LDH and FB-LDH nanocomposites were characterized by X-ray powder diffraction, infrared and thermogravimetric analyses. From drug loading, thermal analysis and X-ray measurements we can decide that coprecipitaion technique is better than reconstruction technique to obtain intercalated monophase nanocomposites. In acidic medium LDH dissolved and the intercalated drug starts to release in a molecular form which is suitable for adsorption. The drug solubility has been investigated before and after intercalation. It has been found that LDH improves the drug solubility and its dissolution rate.

    DOI: 10.1016/j.ejps.2008.08.006

  • Novel peptide is a protein kinase C (PKC)α-specific substrate 査読 国際誌

    Jeong-Hun Kang, Daisuke Asai, Satoshi Yamada, Toita Riki, Jun Oishi, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

    Proteomics   2008年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • High-throughput colorimetric detection of tyrosine kinase inhibitors based on the aggregation of gold nanoparticles 査読 国際誌

    Jun Oishi, Xiaoming Han, Jeong-Hun Kang, Yoji Asami, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

    2008年7月

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    掲載種別:研究論文(学術雑誌)  

  • Hydrogen-bond-assisted syndiotactic-specific radical polymerizations of N-alkylacrylamides: The effect of the N-substituents on the stereospecificities and unusual large hysteresis in the phase-transition behavior of aqueous solution of syndiotactic poly(N-n-propylacrylamide) 査読

    Tomohiro Hirano, Kimihiko Nakamura, Takahiro Kamikubo, Satoshi Ishii, Kanami Tani, Takeshi Mori, Tsuneyuki Sato

    JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY   46 ( 13 )   4575 - 4583   2008年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The radical polymerizations of N-alkylacrylamides, such as N-methyl-(NMAAm), N-n-propyl-(NNPAAm), N-benzyl-(NBnAAm), and N-(1-phenylethyl)acrylamides (NPhEAAm), at low temperatures were investigated in the absence or presence of hexamethylphosphoramide (HMPA) and 3-methyl-3-pentanol (3Me3PenOH), which induced the syndiotactic specificities in the radical polymerization of N-isopropylacrylamide (NIPAAm). In the absence of the syndiotactic-specificity inducers, the syndiotacticities of the obtained polymers gradually increased as the bulkiness of the N-substituents increased. Both HMPA and 3Me3PenOH induced the syndiotactic specificities in the NNPAAm polymerizations as well as in the NIPAAm polymerizations. The addition of 3Me3PenOH into the polymerizations of NMAAm significantly induced the syndiotactic specificities, whereas the tacticities of the obtained polymers were hardly affected by adding HMPA. In the polymerizations of bulkier monomers, such as NBnAAm and NPhEAAm, HMPA worked as the syndiotactic specificity inducer at higher temperatures, whereas 3Me3PenOH hardly influenced the stereospecificity, regardless of the temperatures. The phase-transition behaviors of the aqueous solutions of poly(NNPAAm)s were also investigated. It appeared that the poly (NNPAAm) with racemo dyad content of 70% exhibited unusual large hysteresis between the heating and cooling processes. (C) 2008 Wiley Periodicals, Inc.

    DOI: 10.1002/pola.22797

  • Colorimetric enzymatic activity assay based on "non-crosslinking aggregation" of gold nanoparticles induced by adsorption of substrate peptides 査読 国際誌

    Jun Oishi, Yoji Asami, Takeshi Mori, Jeong-Hun Kang, Takuro Niidome, Yoshiki Katayama

    Biomacromolecules   2008年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • PEG-modified gold nanorods as a photothermal nanodevice for hyperthermia 査読 国際誌

    Takuro Niidome, Yasuyuki Akiyama, Masato Yamagata, Takahito Kawano, Takeshi Mori, Yasuro Niidome, Yoshiki Katayama

    J. Biomater. Sci.-Polym. Ed   2008年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Correlation between phosphorylation ratios by MALDI-TOF MS analysis and enzyme kinetics 査読 国際誌

    Jeong-Hun Kang, Masanori Kuramoto, Akira Tsuchiya, Riki Toita, Daisuke Asai, Yuko T. Sato, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

    Eur. J. Mass Spectrom.,   2008年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • A syngeneic hepatocellular carcinoma model rapidly and simply prepared using a hydrodynamics-based procedure 査読 国際誌

    Jeong-Hun Kang, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

    Vet. J   2008年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • In vivo monitoring of intravenously injected gold nanorods using near-infrared light 査読 国際誌

    Takuro Niidome, Yasuyuki Akiyama, Kohei Shimoda, Takahito Kawano, Takeshi Mori, Yoshiki Katayama, and Yasuro Niidome

    Small   2008年7月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • A short peptide is a protein kinase C (PKC) alpha-specific substrate. 査読 国際誌

    Jeong-Hun Kang, Daisuke Asai, Satoshi Yamada, Riki Toita, Jun Oishi, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

    Proteomics   8 ( 10 )   2006 - 11   2008年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The purpose of this study was to find protein kinase C (PKC) isozyme-specific peptides. A peptide library containing 1772 sequences was designed using Scansite and screened by MALDI-TOF MS and kinase activity assays for PKC isozyme-specificity. A peptide (Alphatomega; H-FKKQGSFAKKK-NH(2)) with high specificity for PKC alpha relative to other isozymes was identified. The peptide was phosphorylated to a greater extent by tissue lysates from B16 melanoma, HepG2, and human breast cancer, which had higher levels of activated PKC alpha, when compared to normal skin, liver, and human breast tissue lysates, respectively. Moreover, addition of Ro-31-7549, an inhibitor with great specificity for PKC alpha, to the phosphorylation reaction caused a dose-dependent reduction in phosphorylation, but no inhibition was identified with the addition of rottlerin and H-89. These results show that this peptide has great potential as a PKC alpha-specific substrate.

    DOI: 10.1002/pmic.200701045

  • Surface plasmon resonance imaging measurements of caspase reactions on peptide microarrays 査読 国際誌

    Yusuke Inoue, Takeshi Mori, Go Yamanouchi, Xiaoming Han, Tatsuhiko Sonoda, Takuro Niidome, Yoshiki Katayama

    Anal. Biochem.   2008年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Evaluation of protein kinase activities of cell lysates using peptide microarrays based on surface plasmon resonance imaging. 査読 国際誌

    Takeshi Mori, Kazuki Inamori, Yusuke Inoue, Xiaoming Han, Go Yamanouchi, Takuro Niidome, Yoshiki Katayama

    Analytical biochemistry   375 ( 2 )   223 - 31   2008年4月

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    記述言語:英語  

    We developed a peptide microarray based on surface plasmon resonance (SPR) imaging for monitoring protein kinase activities in cell lysates. The substrate peptides of kinases were tethered to the microarray surface modified with a self-assembled monolayer of an alkanethiol with triethylene glycol terminus to create a low nonspecific binding surface. The phosphorylation of the substrate peptides immobilized on the surface was detected with the following phosphate specific binders by amplifying SPR signals: anti-phosphotyrosine antibody for tyrosine kinases and Phos-tag biotin (a phosphate-specific ligand with biotin tag) for serine/threonine kinases. Using the microarray, 9 kinds of protein kinases were evaluated as a pattern of phosphorylation of 26 kinds of substrate peptides. The pattern was unique for each protein kinase. The microarray could be used to evaluate the inhibitory activities of kinase inhibitors. The microarray was applied successfully for kinase activity monitoring of cell lysates. The chemical stimuli responsive activity changes of protein kinases in cell lysates could also be monitored by the peptide microarray. Thus, the peptide microarray based on SPR imaging would be applicable to cell-based drug discovery, diagnosis using tissue lysates, and biochemical studies to reveal signal transduction pathways.

    DOI: 10.1016/j.ab.2007.12.011

  • Development of polymeric drug delivery system for recognizing vascular endothelial dysfunction 査読 国際誌

    Kenjiro Ikuta, Takeshi Mori, Tatsuhiro Yamamoto, Takuro Niidome, Hiroaki Shimokawa, Yoshiki Katayama

    Bioorg. Med. Chem.   2008年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Development of polymeric drug delivery system for recognizing vascular endothelial dysfunction 査読 国際誌

    Kenjiro Ikuta, Takeshi Mori, Tatsuhiro Yamamoto, Takuro Niidome, Hiroaki Shimokawa, Yoshiki Katayama

    Bioorg. Med. Chem.   2008年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Optimal surface chemistry for peptide immobilization in on-chip phosphorylation analysis 査読 国際誌

    Kazuki Inamori, Motoki Kyo, Kazuki Matsukawa, Yusuke Inoue, Tatsuhiko Sonoda, Kenji Tatematsu, Katsuyuki Tanizawa, Takeshi Mori, Yoshiki Katayama

    Anal. Chem.   2008年2月

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    掲載種別:研究論文(学術雑誌)  

  • Efficient delivery of siRNA using dendritic poly(L-lysine) for loss-of-function analysis 査読 国際誌

    Yusuke Inoue, Ryosuke Kurihara, Akiko Tsuchida, Mino Hasegawa, Takeyuki Nagashima, Takeshi Mori, Takuro Niidome, Yoshiki Katayama, Osamu Okitsu

    J. Control. Release   2008年1月

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    掲載種別:研究論文(学術雑誌)  

  • Role of plasma as activator and cofactor in phosphorylation by protein kinase C 査読 国際誌

    Jeong-Hun Kang, Daisuke Asai, Jun Oishi, Kenji Kawamura, Riki Toita, Yuhua Jiang, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

    Cell Biochem. Funct   2007年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • 金ナノ粒子の凝集を利用したプロテインキナーゼ活性の評価法

    森 健, 片山 佳樹

    高分子   56 ( 10 )   841 - 841   2007年10月

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    記述言語:日本語  

    Protein Kinase Assay Based on Aggregation of Gold Nanoparticles

    DOI: 10.1295/kobunshi.56.841

  • ANYL 193-New method for in vivo imaging of intracellular signals and its application to cancer imaging 査読

    Jeong-Hun Kang, Yuko Sato, Riki Toita, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   234   2007年8月

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    記述言語:英語  

  • A quantitative peptide array for evaluation of protein kinase activity 国際誌

    Xiaoming Han, Syuhei Shigaki, Takayuki Yamaji, Go Yamanouchi, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

    Anal. Biochem   2007年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Overall interaction of cytosolic proteins with the PEI/DNA complex. 査読 国際誌

    Takayuki Iida, Takeshi Mori, Yoshiki Katayama, Takuro Niidome

    Journal of controlled release : official journal of the Controlled Release Society   118 ( 3 )   364 - 9   2007年4月

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    記述言語:英語  

    Little is known on mechanisms involved in transport of complex of DNA and gene carrier molecules from the cytosol to the nucleus. We aimed to identify cytosolic proteins interacting with the polyethylenimine (PEI)/DNA complex, using 2-D gel electrophoresis and peptide mass fingerprinting. Fifteen proteins including actin, beta-tubulin, and other metabolic proteins were identified. They demonstrated various molecular weights and isoelectric points, and were categorized into 3 groups: early binding, late binding, and transient binding proteins. Protein binding caused DNA release from the PEI/DNA complex with a cation/anion (C/A) ratio of 2, where complex formation was weak. Knowledge on interactions between cytosolic proteins and DNA/carrier complexes will help understand intracellular gene delivery mechanisms.

    DOI: 10.1016/j.jconrel.2006.12.027

  • Mass-tag technology responding to intracellular signals as a novel assay system for the diagnosis of tumor

    J.-H. Kang, A. Han, S. Shigaki, J. Oishi, K. Kawamura, R. Toita, X. M. Han, T. Mori, T. Niidome, Y. Katayama

    J. Am. Soc. Mass Spectr.   2007年3月

  • Stable incorporation of gold nanorods into N-isopropylacrylamide hydrogels and their rapid shrinkage induced by near-infrared laser irradiation. 査読 国際誌

    Atsushi Shiotani, Takeshi Mori, Takuro Niidome, Yasuro Niidome, Yoshiki Katayama

    Langmuir : the ACS journal of surfaces and colloids   23 ( 7 )   4012 - 8   2007年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In this study, we prepared gold nanorod (NR)-embedded N-isopropylacrylamide (NIPAM) hydrogels and studied their volume phase transition behavior induced by near-infrared (near-IR) laser irradiation utilizing the photothermal conversion characteristics of the NRs. When poly(ethylene glycol)-modified NRs were used for the preparation of composite gels, the NRs showed marked dispersion stability in the gel. Near-IR laser irradiation of the gel (cylindrical shape, diameter = 140 microm) under the following conditions, NR concentrations in the gel > or =100 microM and laser irradiation power > or =490 mW, resulted in shrinkage of the gel in the following manner: (1) waist formation around the irradiation spot and (2) growth of the waist along the axial directions of the gel. The gel shrinking induced by near-IR irradiation occurred much more rapidly than that afforded by a temperature jump, because the former was not accompanied by the skin layer formation, which disturbs the rapid shrinking of the gels. When a composite gel containing the model drug (rhodamine-labeled dextran) was irradiated with a near-IR laser, the rapid release of the drug was observed. Taking advantage of the high spatial resolution of the irradiation point, we further achieved the irradiation-point-specific release of the drug from one such gel.

    DOI: 10.1021/la0627967

  • A peptide microarray for the detection of protein kinase activity in cell lysate. 査読

    Syuhei Shigaki, Takayuki Yamaji, Xiaoming Han, Go Yamanouchi, Tatsuhiko Sonoda, Osamu Okitsu, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry   23 ( 3 )   271 - 5   2007年3月

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    記述言語:英語  

    DNA microarray enables the analysis of DNA or mRNA expression levels, but it has not been possible to completely understand life using obtained information. Consequently, protein or peptide arrays have attracted much interest. Since the development of a practical protein microarray is still far away in light of handling difficulties, the peptide microarray is a promising tool for analyzing protein functions. We have developed a peptide microarray to detect protein kinase activity in cell lysate. All substrate peptides for kinases were immobilized chemoselectively on amino-coated glass slides. After phosphorylation of the immobilized peptides, phosphorylation was detected by fluorescence imaging. We detected the protein kinase activities, including that in cell lysate, in response to drug stimulation. Therefore, this peptide microarray would be useful for a high-throughput kinase assay of intracellular signals and would be applicable to drug screening.

    DOI: 10.2116/analsci.23.271

  • Stable incorporation of gold nanorods into N-isopropylacrylamide hydrogels and their rapid shrinkage induced by near-IR laser irradiation

    A. Shiotani, T. Mori*, T. Niidome, Y. Niidome, Y. Katayama

    Langmuir   2007年2月

  • Temperature-responsive poly(dehydroalanine)s: Diversifying phase transition temperatures utilizing alpha,alpha-disubstituted motif 査読

    Takeshi Mori, Suguru Beppu, Isao Fukushima, Toru Kobayashi, Keiji Minagawa, Masami Tanaka, Takuro Niidome, Yoshiki Katayama

    CHEMISTRY LETTERS   36 ( 2 )   334 - 335   2007年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The temperature-responsive poly(dehydroalanine derivative)s having two substituents at the alpha-position were successfully synthesized. By varying the combination of the structures of the two substituents, the relatively large diversity of their phase transition temperatures could be achieved.

    DOI: 10.1246/cl.2007.334

  • "Threading" of beta-sheet peptides via radical polymerization. 査読 国際誌

    Takeshi Mori, Shiro Yasutake, Hideki Inoue, Keiji Minagawa, Masami Tanaka, Takuro Niidome, Yoshiki Katayama

    Biomacromolecules   8 ( 2 )   318 - 21   2007年2月

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    記述言語:英語  

    A nonamer peptide containing a diene group in the center of the sequence was synthesized. When the peptide forms an antiparallel beta-sheet, the diene groups align ca. 5 A apart on the beta-sheet. The diene groups successfully photopolymerized without distorting the beta-sheet structure. The obtained beta-sheet showed high stability against acid denaturation and addition of 1,1,1,3,3,3-hexafluoroisopropanol.

    DOI: 10.1021/bm060835n

  • A peptide microarray for the detection of protein kinase activity in cell lysate

    S. Shigaki, T. Yamaji, X. Han, G. Yamanouchi, T. Sonoda, O. Okitsu, T. Mori, T. Niidome, Y. Katayama

    Anal. Sci.   2007年2月

  • Structural advantage of dendritic poly(L-lysine) for gene delivery into cells

    M. Yamagata, T. Kawano, K. Shiba, T. Mori, Y. Katayama, T. Niidome

    Bioorg. Med. Chem.   2007年2月

  • "Threading" of beta-Sheet Peptides via Radical Polymerization

    T. Mori*, S. Yasutake, H. Inoue, K. Minagawa, M. Tanaka, T. Niidome, Y. Katayama

    Biomacromolecules   2007年1月

  • Structural advantage of dendritic poly(L-lysine) for gene delivery into cells 査読 国際誌

    Masato Yamagata, Takahito Kawano, Kouhei Shiba, Takeshi Mori, Yoshiki Katayama, Takuro Niidome

    BIOORGANIC & MEDICINAL CHEMISTRY   15 ( 1 )   526 - 532   2007年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    This study aimed to investigate the relationships between structures of gene carrier molecules and their activities for gene delivery into cells. We compared 2 types of poly(L-lysine) as carriers, that is, dendritic poly(L-lysine) (KG6) and linear poly(L-lysine) (PLL). KG6 formed a neutral DNA complex, and its DNA compaction level was weaker than that of PLL. The amount of DNA binding and uptake into cells mediated by PLL was 4-fold higher than that with KG6. However, KG6-mediated gene expression was 100-fold higher than that by PLL. Since pK(a) values of terminal amines of KG6 were lowered even though small amounts of DNA were internalized into cells, sufficient DNA amounts for effective gene expression escaped to the cytosol due to the proton sponge effect in the endosome. In addition, weakly compacted DNA with KG6 was advantageous in accessing RNA polymerase in the cell nucleus. On the other hand, PLL did not show the proton sponge effect in the endosome and resulted in strong compaction of DNA. Even though large DNA amounts were internalized into cells, most of the DNA would not take part in gene expression systems in the nucleus. Amount of induced cytokine production after intravenous injection of DNA complexes with KG6 and PLL was low, and was similar to the case when DNA was injected alone. Therefore, no significant difference in effects on cytokine production was observed between KG6 and PLL. (c) 2006 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.bmc.2006.09.033

  • Mass-tag technology responding to intracellular signals as a novel assay system for the diagnosis of tumor. 査読 国際誌

    Jeong-Hun Kang, Yoshiki Katayama, Aishan Han, Shuhei Shigaki, Jun Oishi, Kenji Kawamura, Riki Toita, Xiao Ming Han, Takeshi Mori, Takuro Niidome

    Journal of the American Society for Mass Spectrometry   18 ( 1 )   106 - 12   2007年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    A novel mass spectrometry-based assay system for determining protein kinase activity employing mass-tagged substrate peptide probes was used for the diagnosis of tumors. Two peptide probes (H-type and D-type) were synthesized containing the same substrate peptide sequence for protein kinase C (PKC). The molecular weights of the two probes differ because of the incorporation of deuterium into the acetyl groups of the D-type probe. The lysates of the normal and tumor tissue were prepared and reacted with the H- and D-type peptide probes, respectively. The PKC activities of the normal and tumor tissues can be compared simply and directly by calculating the phosphorylated ratio to each peptide probe, obtained from the peak intensity of the mass spectrum after mixing of the two reaction solutions. The phosphorylation ratio for the reaction of the H-type peptide probe with the tumor tissue lysate (B16 melanoma) was more than three times higher than that of the D type peptide probe with the normal skin tissue lysate. These results show that the novel assay system for detecting protein kinase activity using mass-tag technology can be a simple and useful means to profile protein kinase activity for cell or tissue lysate samples, and can be applied to the diagnosis of tumors.

    DOI: 10.1016/j.jasms.2006.09.004

  • Overall interaction of cytosolic proteins with the PEI/DNA complex

    T. Iida, T. Mori, Y. Katayama, T. Niidome

    J. Control Release   2007年1月

  • Temperature-responsive Poly(dehydroalanine)s: Diversifying Phase Transition Temperatures Utilizing alpha,alpha-Disubstituted Motif

    T. Mori*, S. Beppu, I. Fukushima, T. Kobayashi, K. Minagawa, M. Tanaka, T. Niidome, Y. Katayama

    Chem. Lett.   2007年1月

  • A protein kinase signal-responsive gene carrier modified RGD peptide. 査読 国際誌

    Jun Oishi, Moeko Ijuin, Tatsuhiko Sonoda, Jeong-Hun Kang, Kenji Kawamura, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

    Bioorganic & medicinal chemistry letters   16 ( 22 )   5740 - 3   2006年11月

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    記述言語:英語  

    We have previously reported artificial gene-regulation systems responding to cyclic AMP-dependent protein kinase (PKA) using a cationic polymer. However, this polymer alone cannot deliver any gene into living cells. In the present work, we modified the signal-responsive polymer to the RGD peptide for the introduction of a polymer/DNA complex into living cells and succeeded in regulating the gene expression responding to intracellular PKA activation.

    DOI: 10.1016/j.bmcl.2006.08.096

  • Polymerization of diacetylene using beta-sheet as a template 査読

    Takeshi Mori, Yoichi Fukawa, Kenji Shimoyama, Keiji Minagawa

    INTERNATIONAL JOURNAL OF MODERN PHYSICS B   20 ( 25-27 )   3872 - 3877   2006年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    In the parallel and anti-parallel beta-sheet structures, hydrogen bonding arises between the amide bonds of the peptide chains to arrange them with a distance of ca. 5 angstrom. That distance matched with the repeating unit distance of polydiacetylene. In this study, the effectiveness of the beta-sheet as a template for the polymerization of diacetylene was examined by using diacetylene-introduced oligopeptides. The diacetylene-introduced amino acid (Thr(DA)) was synthesized from L-threonine. Though peptides Ac-Thr(DA)-NHMe and Ac-[Thr(DA)](2)-NHMe formed anti-parallel beta-sheet, they showed slight or no polymerization in both of the solid and the solution states. On the other hand, Ac-[Thr(DA)](5)-NHMe and 11mer peptide with a Thr(DA) in the center of the sequence contained anti-parallel beta-sheet structure and formed polydiacetylene of high degree of polymerization with high conversion during the cleavage process of the peptide from resin in the solution. This result indicated that the preorganization of the peptide through the beta-sheet formation was necessary for the polymerization of diacetylene group. Thus, the beta-sheet motif was effective template for the polymerization of diacetylene.

    DOI: 10.1142/S0217979206040519

  • Ternary electrorheological fluids with composite particles dispersed in liquid blends 査読

    Keiji Minagawa, Yasunori Aoki, Takeshi Mori

    INTERNATIONAL JOURNAL OF MODERN PHYSICS B   20 ( 25-27 )   3987 - 3992   2006年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The electrorheological (ER) properties were studied with ternary suspensions containing solid and liquid dispersoids in silicone oil (DMS). The solid dispersoid used was polyether/montmorillonite nanocomposite particles in which polyether molecules were intercalated between the layers of montmorillonite. The liquid dispersoid was urethane-modified polyether prepared from 4,4'-diphenylmethane diisocyanate (MDI) and poly(propylene glycol) (PPG). The steady shear viscosity and dynamic viscoelasticity were measured with a rotating parallel disc rheometer equipped with ER measurement system. The binary and ternary mixtures of these solid and liquid dispersoids with DMS, i.e. composite particle/DMS suspension, polyether/DMS liquid blend, and ternary blend containing both dispersoids, showed an increase of viscosity in response to an electric field (positive ER effect). The ER effect of a ternary suspension containing the particles and polyether liquids was larger than those of binary suspension or blend with one of these dispersoids. The ER effect was found to be improved by combination of the solid and liquid dispersoids, although the recovery time of viscosity after removing the electric field became rather longer, which would be due to the partly irreversible macroscopic aggregation of the dispersoids.

    DOI: 10.1142/S0217979206040738

  • Soluble-Insoluble-Soluble Transitions of Aqueous Poly(N-vinylacetamide-co-acrylic acid) Solutions 査読 国際誌

    T. Mori, M. Nakashima, Y. Fukuda, K. Minagawa, M. Tanaka, Y. Maeda

    Langmuir   2006年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Multi-step precipitation separation system using mixture of thermosensitive polymers 査読

    T Mori, H Mori, K Minagawa, M Tanaka

    POLYMER BULLETIN   56 ( 2-3 )   211 - 220   2006年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Multi-step precipitation separation system was developed by using aqueous mixtures of some thermosensitive polymers. The following three polymers were used here; poly(N-n-propylacrylamide), poly(N-isopropylacrylamide), and poly(N-isopropylmethacrylamide). A mixture of the three polymers showed three endothermic peaks, and the peak top temperatures were almost consistent with that of the each polymer solution. The polymers were purified by thermal precipitation to obtain fractions which can respond in narrow temperature ranges prior to use. In the case of the precipitation separation of two polymers mixtures, purities of the obtained precipitate and supernatant fractions became high comparing with the case in which the unpurified polymers were used. Parts of the polymers which were not the precipitation targets were also precipitated by the separation procedures. This was caused not only by insolubilization of the non-targeted polymers due to their phase transitions but also by their non-specific entanglement with the targeted polymers. The purities of the fractions also improved when the difference of the phase transition temperature between two polymers was large enough to avoid the coprecipitation. In the case of the precipitation separation of mixtures of the three polymers, purities of each fraction also improved when the purified polymers were used.

    DOI: 10.1007/s00289-005-0486-y

  • Preparation of Nano- and Micro-Particles through Self-Assembly of Azobenzen-Pendent Ionomers 査読 国際誌

    T. Mori, K. Yuyama, K. Narita, K. Minagawa, M. Haraguchi, M. Tanaka

    Journal of Applied Polymer Science   2006年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Phosphorylation of Rho-associated kinase (Rho-kinase/ROCK/ROK) substrates by protein kinases A and CPhosphorylation of Rho-associated kinase (Rho-kinase/ROCK/ROK) substrates by protein kinases A and C 査読 国際誌

    J.-H. Kang, Y. Jiang, R. Toita, J. Oishi, K. Kawamura, A. Han, T. Mori, T. Niidome, M. Ishida, K. Tatematsu, K. Tanizawa, Y. Katayama

    Biochimie   2006年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Effect of Alkyl Substituents Structures and Added Ions on the Phase Transition of Polymers and Gels Prepared from Methyl 2-Alkylamidoacrylates 査読 国際誌

    T. Mori, M. Hamada, T. Kobayashi, H. Okamura, K. Minagawa, S. Masuda, M. Tanaka

    Journal of Polymer Science, Part A: Polymer Chemistry Edition   2005年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Self-assembly of Oligo(p-phenylenevinylene)-block-Poly(ethylene oxide) in Polar Media and Solubilization of an Oligo(p-phenylenevinylene) Homooligomer inside the Assembly 査読 国際誌

    T. Mori, T. Watanabe, K. Minagawa, M. Tanaka

    Journal of Polymer Science, Part A: Polymer Chemistry Edition   2005年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Alternative Approach to the Design of Thermosensitive Polymer: The Addition of Hydrophobic Groups to Ends of Hydrophilic Polyether 査読 国際誌

    T. Mori, Y. Shiota, K. Minagawa, M. Tanaka

    Journal of Polymer Science, Part A: Polymer Chemistry Edition   2005年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Parallel beta-sheet as a Novel Template for Polymerization of Diacetylene 査読 国際誌

    T. Mori, K. Shimoyama, Y. Fukawa, K. Minagawa, M. Tanaka

    Chemistry Letters   2005年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Thermosensitive Copolymers Having Soluble and Insoluble Monomer Units, Poly(N-vinylacetamide-co-methyl acrylate)s: Effect of Additives on Their Lower Critical Solution Temperatures 査読 国際誌

    T. Mori, Y. Fukuda, H. Okamura, K. Minagawa, S. Masuda, M. Tanaka

    Journal of Polymer Science, Part A: Polymer Chemistry Edition   2004年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Temperature-Responsive Formation of Colloidal Nanoparticles from Poly(N-isopropylacrylamide) Grafted with Single-Stranded DNA 査読 国際誌

    T. Mori, M. Maeda

    Langmuir   20 ( 2 )   313 - 319   2004年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1021/la0356194

  • Stability Change of DNA-Carrying Colloidal Particle Induced by Hybridization with Target DNA 査読 国際誌

    T. Mori, M. Maeda

    Polymer Journal   34 ( 8 )   624 - 628   2002年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1295/polymj.34.624

  • Effects of Salts and Copolymer Composition on Lower Critical Solution Temperature of Poly(methyl 2-acetamidoacrylate-co-methyl methacrylate) Solution 査読 国際誌

    H. Okamura, Y. Morihara, S. Masuda, K. Minagawa, T. Mori, M. Tanaka

    Journal of Polymer Science, Part A: Polymer Chemistry Edition   2002年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • A Novel Thermosensitive Polymer, Poly(methyl 2-propionamidoacrylate), with Geminal Substituents 査読 国際誌

    H. Okamura, T. Mori, K. Minagawa, S. Masuda, M. Tanaka

    Polymer   2002年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Thermosensitive Properties of a Novel Poly(methyl 2-acetamidoacrylate-co-methyl acrylate) 査読 国際誌

    H. Okamura, S. Masuda, K. Minagawa, T. Mori, M. Tanaka

    European Polymer Journal   2002年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Chain Behavior in Model Homogeneous ER Fluids Depending on Temperature 査読 国際誌

    H. Okamura, K. Suzuki, T. Mori, K. Minagawa, S. Masuda, M. Tanaka

    International Journal of Modern Physics B   2002年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • A Novel Thermosensitive Poly(methyl 2-acetamidoacrylate) 査読 国際誌

    H. Okamura, T. Maruyama, S. Masuda, K. Minagawa, T. Mori, M. Tanaka

    Journal of Polymer Research   2002年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Novel Electrorheological Materials with Ternary Blends Containing Polyethers and Urethanes 査読 国際誌

    K. Minagawa, T. Kanno, H. Okamura, T. Mori, S. Masuda, H. Takagi, K. Koyama, M. Tanaka

    International Journal of Modern Physics B   2002年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Electric-Field Response of the Structure and Rheological Property of Silicone/Polyether Blends 査読 国際誌

    K. Minagawa, K. Saitoh, H. Okamura, T. Mori, S. Masuda, M. Tanaka

    International Journal of Modern Physics B   2002年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Formation of DNA-Carrying Colloidal Particle from Poly(N-isopropylacrylamide)-graft-DNA Copolymer and Its Assembly through Hybridization 査読 国際誌

    T. Mori, M. Maeda

    Polymer Journal   33 ( 10 )   830 - 833   2001年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Sequence-specific Affinity Precipitation of Oligonucleotide Using Poly(N-isopropylacrylamide) -Oligonucletide Conjugate 査読 国際誌

    T. Mori, D. Umeno, M. Maeda

    Biotechnolgy and Bioengineering   72 ( 3 )   261 - 268   2001年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/1097-0290(20010205)72:3<261::AID-BIT2>3.0.CO;2-7

  • A protein kinase signal-responsive gene carrier modified RGD peptide 査読 国際誌

    J. Oishi, M. Ijuin, T. Sonoda, J.-H. Kang, K. Kawamura, T. Mori, T. Niidome, Y. Katayama

    Bioorg. Med. Chem. Lett.   1900年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Reversal of efflux of an anticancer drug in human drug-resistant breast cancer cells by inhibition of protein kinase C alpha activity 査読 国際誌

    C.W. Kim, D. Asai, J.-H. Kang, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

    Tumor Biol   1900年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • A Rapid and Quantitative Detection of Cellular Protein Kinase Activity Based on MALDI-TOF-MS

    Y. Otsubo, H. Ikeda, J. Kamimoto, T. Niidome, Takeshi Mori, Yoshiki Katayama

    Chem. Lett.   43   658   1900年

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    記述言語:英語  

▼全件表示

書籍等出版物

  • ドラッグデリバリーシステム-バイオ医薬品創成に向けた組織、細胞内、核内送達技術の開発-

    森 健(担当:共著)

    2018年10月 

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    記述言語:日本語   著書種別:学術書

  • Intracellular Delivery

    Takeshi Mori, Yoshiki Katayama(担当:共著)

    2014年5月 

     詳細を見る

    記述言語:英語   著書種別:学術書

  • Versatile Nanocompsite Formulation System of Non-Steroidal Anti-Inflammatory Drugs of the Arylalkanoic Acids, In Advances in Nanocomposite Technology; A. Hashim Eds.; In Tech 2011; p335-360.

    M. R. Berver, I. H. Hafez, K. Minagawa, T. Mori, M. Tanaka

    2012年5月 

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    著書種別:一般書・啓蒙書

  • Biomarkers for Melanoma Diagnosis and the Technologies Used to Identify Them, In Breakthroughs in Melanoma Research; Y. Tanaka Eds.; In Tech 2011; p389-412.

    T. Mori, J.-H. Kang

    2012年5月 

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    著書種別:一般書・啓蒙書

  • Biomedical Diagnositc Science and Technology

    M. Maeda, T. Mori, D. Umeno, Y. Katayama(担当:共著)

    Marcel Dekker Inc.  2002年1月 

     詳細を見る

    担当ページ:“Diagnostic Polymer Reagents Carrying DNA”, pp 215-226   記述言語:英語   著書種別:学術書

講演・口頭発表等

  • 金ナノ粒子を用いたハイスループットキナーゼ活性評価法

    森 健、大石潤、朝見陽次、新留琢郎、片山佳樹

    化学とマイクロナノ研究会  2007年5月 

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    開催年月日: 2008年7月

    国名:日本国  

  • 蛍光型ペプチド・マイクロアレイを用いるヒト・キノーム解析

    森 健、韓暁明、山之内豪、新留琢郎、片山佳樹

    化学とマイクロナノシステム研究会  2007年10月 

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    開催年月日: 2007年10月

    国名:日本国  

  • 蛍光型ペプチド・マイクロアレイを用いるヒト・キノーム解析

    森 健、韓暁明、山之内豪、新留琢郎、片山佳樹

    化学とマイクロナノシステム研究会  2007年10月 

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    開催年月日: 2007年10月

    国名:日本国  

  • 蛍光型ペプチド・マイクロアレイを用いるヒト・キノーム解析

    森 健、韓暁明、山之内豪、新留琢郎、片山佳樹

    化学とマイクロナノシステム研究会  2007年10月 

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    開催年月日: 2007年10月

    国名:日本国  

  • Homogeneous kinase activity screeining for cell lysates utilizing gold nanoparticle aggregation

    Takeshi Mori, Jun Oishi, Yoji Asami, Jeong-Hun Kang, Takuro Niidome, Yoshiki Katayama

    American Chemical Society  2007年8月 

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    開催年月日: 2007年8月

    国名:日本国  

  • 金ナノ粒子の凝集を指標とする比色型キナーゼ活性評価法

    森健、大石潤、朝見陽次、姜貞勲、新留琢郎、片山佳樹

    バイオ高分子研究会  2007年7月 

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    開催年月日: 2007年7月

    国名:日本国  

  • “偽可逆的”熱硬化性を有するポリビニルアミノ酸の開発

    森 健、新留琢郎、片山佳樹、別府卓、南川慶二、田中正己

    第35回医用高分子シンポジウム  2006年8月 

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    会議種別:口頭発表(一般)  

    開催地:東京  

  • ポリ(N-ビニルアセトアミド-co-アクリル酸)水溶液におけるLCSTおよびUCSTの発現メカニズム

    中島将史、森 健、南川慶二、田中正己

    第54回高分子学会年次大会  2005年5月 

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    会議種別:口頭発表(一般)  

    開催地:横浜市   国名:日本国  

  • ナトリウム塩存在下での2-アセトアミドアクリル酸メチルの超音波重合

    和田慎也、森 健、南川慶二、田中正己

    第54回高分子学会年次大会  2005年5月 

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    会議種別:口頭発表(一般)  

    開催地:横浜市  

  • ポリ(デヒドロアラニン)水溶液の転移の共同性に与える疎水置換基および添加イオンの効果

    森 健、福島功、南川慶二、田中正己

    第54回高分子学会年次大会  2005年5月 

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    会議種別:口頭発表(一般)  

    開催地:横浜市  

  • ペプチドβ-シート上でのラジカル重合反応制御

    森 健,安武 志朗,南川 慶二,田中 正己

    第54回高分子討論会  2005年9月 

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    会議種別:口頭発表(一般)  

    開催地:山形市  

  • アシル及びエステル部に構造異性を有するポリ(デヒドロアラニン)水溶液の熱応答挙動

    森 裕紀,福島 功,森 健,南川 慶二,田中 正己

    第54回高分子討論会  2005年9月 

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    会議種別:口頭発表(一般)  

    開催地:山形市  

  • Polymerization of Diacetylene Using beta-sheet as a Template 国際会議

    T. Mori, Y. Fukawa, K. Shimoyama, K. Minagawa, M. Tanaka

    Advanced Materials Development & Performance Conference  2005年7月 

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    会議種別:口頭発表(一般)  

    開催地:Auckland   国名:ニュージーランド  

  • alpha-Disubstituted Thermosensitive Vinyl Polymers; Poly(alkyl 2-alkylamidoacrylate)s 国際会議

    T. Mori, I. Fukushima, T. Kobayashi, H. Okamura, K. Minagawa, M. Tanaka

    Advanced Materials Development & Performance Conference  2005年7月 

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    会議種別:口頭発表(一般)  

    開催地:Auckland   国名:ニュージーランド  

  • Ternary electrorheological fluids with composite particles dispersed in liquid blends 国際会議

    K. Minagawa, Y. Aoki, T. Mori, M. Tanaka

    Advanced Materials Development & Performance Conference  2005年7月 

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    会議種別:口頭発表(一般)  

    開催地:Auckland   国名:ニュージーランド  

  • Ultrasonic-induced polymerization of methyl 2-acetamidoacrylate in the presence of sodium salts 国際会議

    K. Minagawa, S. Wada, T. Mori, M. Tanaka

    Advanced Materials Development & Performance Conference  2005年7月 

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    会議種別:口頭発表(一般)  

    開催地:Auckland   国名:ニュージーランド  

  • Effect of Alkyl Substituents Structures and Added Ions on the Phase Transition of Polymers and Gels Prepared from Methyl 2-Alkylamidoacrylates 国際会議

    T. Mori, I. Fukushima, K. Minagawa, M. Tanaka

    International Polymer Conference  2005年7月 

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    会議種別:口頭発表(一般)  

    開催地:福岡市  

  • beta-sheet as a Novel Template for Polymerization of Diacetylene 国際会議

    T. Mori, K. Minagawa, M. Tanaka M. Maeda

    Pacifichem  2005年12月 

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    会議種別:口頭発表(一般)  

    開催地:Honolulu   国名:アメリカ合衆国  

  • Intelligent Polymer Cement; Thermosensitive Polymers with Abnormal Hysteresis 国際会議

    T. Mori, H. Mori, S. Beppu, T. Makimura, K. Minagawa, M. Tanaka, T. Niidome, Y. Katayama

    Controlled Release Society 33rd Annual Meeting  2006年7月 

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    会議種別:口頭発表(一般)  

    開催地:Viena   国名:オーストリア共和国  

  • ポリビニルアミノ酸水溶液の異常な熱履歴現象

    森 健、新留琢郎、片山佳樹、槙村卓巳、森裕紀、南川慶二、田中正己

    第55回高分子学会年次大会  2006年5月 

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    会議種別:口頭発表(一般)  

    開催地:名古屋  

  • MMP-2応答性ドラッグキャリアーの開発

    西健太郎、森 健、新留琢郎、片山佳樹

    第55回高分子学会年次大会  2006年5月 

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    会議種別:口頭発表(一般)  

    開催地:名古屋  

  • アルキル鎖長の異なるポリ(デヒドロアラニン)水溶液の熱応答挙動

    槙村卓巳、南川慶二、森 健、田中正己

    第55回高分子学会年次大会  2006年5月 

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    会議種別:口頭発表(一般)  

    開催地:名古屋  

  • インテリジェントポリマーセメントの開発

    森 健、新留琢郎、片山佳樹、別府卓、槙村卓巳、南川慶二、田中正己

    第22回日本DDS学会  2006年7月 

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    会議種別:口頭発表(一般)  

    開催地:東京  

  • EGFR発現肺癌に対する新規抗体薬物複合体の開発

    井上 博之, 生長 幸之助, 田川 寛, 井形 文保, 中尾 明, 海老 規之, 森 健, 藤田 昌樹

    肺癌  2023年10月  (NPO)日本肺癌学会

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    記述言語:日本語  

▼全件表示

MISC

  • 化学に基づく細胞のトランスフォーメーション

    森 健

    化学と工業   2014年11月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 双性イオン型ポリマーは真のステルスポリマーか? 査読

    石橋賢汰, 森 健

    バイオマテリアル   2022年6月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 抗原特異的な免疫寛容を誘導する医薬品の設計, 査読

    劉 一イ・森 健

    Drug Delivery System   2022年6月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 細胞・組織のバイオ分析における増感酵素 査読

    金子 諒右, 森 健, 片山 佳樹

    分析化学   2022年6月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 高分子型蛍光プローブによるプロテインキナーゼの活性検出

    塩崎秀二郎, 戸井田力, 森健, 新留琢郎, 片山佳樹

    日本バイオマテリアル学会シンポジウム予稿集   2012年11月

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    記述言語:日本語  

    高分子型蛍光プローブによるプロテインキナーゼの活性検出

  • 細胞内シグナル応答型遺伝子医薬送達システムの複合体安定化の試み

    串尾聡之, 登貴信, 中村雄太, 戸井田力, 森健, 新留琢郎, 片山佳樹

    高分子学会予稿集(CD-ROM)   2012年9月

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    記述言語:日本語  

    細胞内シグナル応答型遺伝子医薬送達システムの複合体安定化の試み

  • ペプチドマイクロアレイによる細胞内プロテインキナーゼの網羅的解析

    森 健

    分析化学   2012年8月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 細胞内シグナルに応答したsiRNAデリバリーシステムの開発

    川並寛幸, イ ウンキョン, 李翠翆, 戸井田力, 森健, 新留琢郎, 片山佳樹

    日本化学会西日本大会講演要旨集   2011年11月

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    記述言語:日本語  

    細胞内シグナルに応答したsiRNAデリバリーシステムの開発

  • 高分子‐ペプチド複合体による遺伝子の転写制御

    倉本政則, 戸井田力, 塩崎秀二郎, 森健, 新留琢郎, 片山佳樹

    高分子学会予稿集(CD-ROM)   2011年9月

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    記述言語:日本語  

    高分子‐ペプチド複合体による遺伝子の転写制御

  • ポリマー/ペプチドコンジュゲートを用いるがんイメージング剤―前立腺がん特異的プロテアーゼ蛍光プローブ

    倉本政則, 戸井田力, 塩崎秀二郎, 森健, 新留琢郎, 片山佳樹

    高分子学会予稿集(CD-ROM)   2011年9月

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    記述言語:日本語  

    ポリマー/ペプチドコンジュゲートを用いるがんイメージング剤―前立腺がん特異的プロテアーゼ蛍光プローブ

  • The combination of drug or gene delivery system responding to cellular signals (D-RECS) and sonoporation system for effective and safe gene delivery

    A. Tsuchiya, T. Mori, T. Mori, Y. Naritomi, J. H. Kang, D. Asai, T. Niidome, T. Niidome, Y. Endo, R. Suzuki, Y. Negishi, K. Maruyama, Y. Katayama, Y. Katayama

    Materials Research Society Symposium Proceedings   2010年12月

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    記述言語:その他  

    We have developed new gene expression-regulating polymer that can activate transgene expression in response to target intracellular signals. Here, we tried applying sonoporation system to this gene regulation system to enhance the gene expression efficacy. Sonoporation is the method for effective gene transfection in vitro and in vivo. Therefore, the method might enhance the transfection efficiency in our polymer and realize an efficient and safe gene delivery system. Results suggested that the combination of our polymer and sonoporation could improve the gene expression compared to the system using only our polymer that transfers genes into cells via endocytosis. It also kept the ability of the gene regulation responding to cellular signals. © 2010 Materials Research Society.

  • ペプチドグラフト型LPEIを用いたD‐RECS法における癌特異性の向上

    川並寛幸, 戸井田力, 新堀武士, 森健, 新留琢郎, 片山佳樹

    日本化学会西日本大会講演要旨集   2010年11月

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    記述言語:日本語  

    ペプチドグラフト型LPEIを用いたD‐RECS法における癌特異性の向上

  • 離れたドメインの共同性-タンパク質はミスフォールディング回避へと進化した

    森 健

    2010年11月

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    記述言語:その他  

  • がん診断を目指した蛍光物質含有超音波造影剤の開発

    小林優太, 土谷享, 村田正治, 森健, 新留琢郎, 橋爪誠, 片山佳樹

    日本バイオマテリアル学会大会予稿集   2010年11月

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    記述言語:日本語  

    がん診断を目指した蛍光物質含有超音波造影剤の開発

  • The effect of PEG grafted on gold nanorods and their injection dose on biodistribution in tumor-bearing mice

    Yasuyuki Akiyama, Takeshi Mori, Takeshi Mori, Yoshiki Katayama, Yoshiki Katayama, Takuro Niidome, Takuro Niidome, Takuro Niidome

    Materials Research Society Symposium Proceedings   2010年10月

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    記述言語:その他  

    Gold nanorods have strong surface plasmon band in the near-infrared region, at which light penetrates deeply into tissues, and convert the absorbed light energy into heat. Therefore, gold nanorods are expected to act as an effective contrast agent for in vivo bioimaging and as a photosensitizer for photothermal therapy. In order to efficiently achieve these applications without side effects, gold nanorods should be selectively accumulated in the target organ. In this study, we optimized the length of the poly (ethylene glycol) (PEG) chain to stabilize the PEG-modified gold nanorods in blood circulation, and investigated the effects of PEG grafting level and injection dose on the biodistribution and enhanced permeability and retention (EPR) effect after intravenous injection into mice. PEG 5,000 - , PEG 10,000 - and PEG 20,000 - modified gold nanorods showed higher blood circulation stability compared with PEG 2,000 - modifed gold nanorods. Higher PEG grafting levels were advantageous for the reticuloendothelial system (RES) avoidance and suppression of aggregation of the gold nanorods in the blood circulation. Modification with a PEG 5,000 : gold molar ratio of 1.5 was sufficient to show both prolonged circulation and the EPR effect. When the injection dose was increased above 39.0 μg of gold, the RES uptake in the liver was saturated and surplus gold nanorods were distributed to other tissues, especially the spleen and tumor. This information is important key to provide the successful application of gold nanorods in the field of nanomedicine. © 2010 Materials Research Society.

  • がん細胞特異的に遺伝子を活性化するシグナル応答型遺伝子キャリヤー

    戸井田力, 富山哲朗, 新堀武士, 森健, 新留琢郎, 片山佳樹

    化学関連支部合同九州大会・外国人研究者交流国際シンポジウム講演予稿集   2010年7月

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    記述言語:日本語  

    がん細胞特異的に遺伝子を活性化するシグナル応答型遺伝子キャリヤー

  • ペプチド複合体を用いるプロテインキナーゼ計測用蛍光分子プローブ

    古賀春香, 戸井田力, 森健, 新留琢郎, 片山佳樹

    高分子学会予稿集(CD-ROM)   2010年5月

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    記述言語:日本語  

    ペプチド複合体を用いるプロテインキナーゼ計測用蛍光分子プローブ

  • がん細胞内の異常シグナルを利用した遺伝子デリバリーシステム

    富山哲朗, KANG Jeong‐Hun, 戸井田力, 新堀武士, 森健, 新留琢郎, 片山佳樹

    日本バイオマテリアル学会大会予稿集   2009年11月

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    記述言語:日本語  

    がん細胞内の異常シグナルを利用した遺伝子デリバリーシステム

  • ペプチドアレイを用いた表面プラズモン共鳴(SPR)イメージングによる網羅的なOn‐chipリン酸化解析への展開

    稲森和紀, 京基樹, 松川和樹, 井上雄介, 園田達彦, 立松健司, 谷澤克行, 森健, 新留琢郎, 片山佳樹

    日本化学会バイオテクノロジー部会シンポジウム講演要旨集   2009年9月

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    記述言語:日本語  

    ペプチドアレイを用いた表面プラズモン共鳴(SPR)イメージングによる網羅的なOn‐chipリン酸化解析への展開

  • プロテインキナーゼ活性検出のための蛍光プローブの作製と機能評価

    古賀春香, 戸井田力, 富山哲朗, KANG Jeong‐Hun, 森健, 新留琢郎, 片山佳樹

    日本化学会バイオテクノロジー部会シンポジウム講演要旨集   2009年9月

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    記述言語:日本語  

    プロテインキナーゼ活性検出のための蛍光プローブの作製と機能評価

  • 細胞内シグナル酵素応答型ポリマーによる遺伝子発現制御

    富山哲朗, KANG Jeong‐Hun, 戸井田力, 古賀春香, 森健, 新留琢郎, 片山佳樹

    高分子学会予稿集(CD-ROM)   2009年5月

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    記述言語:日本語  

    細胞内シグナル酵素応答型ポリマーによる遺伝子発現制御

  • クリックケミストリーによるポリマーへの修飾

    戸井田力, KANG Jeong‐Hun, 古賀春香, 富山哲朗, 森健, 新留琢郎, 片山佳樹

    高分子学会予稿集(CD-ROM)   2009年5月

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    記述言語:日本語  

    クリックケミストリーによるポリマーへの修飾

  • ソノポレーション法と細胞内シグナル応答型遺伝子キャリアーを併用した遺伝子デリバリー

    土谷享, 成富友紀, KANG Jeong‐Hun, 森健, 新留琢郎, 遠藤葉子, 根岸洋一, 鈴木亮, 丸山一雄, 片山佳樹

    日本化学会講演予稿集   2009年3月

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    記述言語:日本語  

    Gene delivery system use of sonoporation with gene carriers responding to cellular signals

  • Development of Gold Nanorods That Accumulatate into Tumor

    NIIDOME Takuro, OHGA Akira, NIIDOME Yasuro, MORI Takeshi, KATAYAMA Yoshiki

    Peptide science : proceedings of the ... Japanese Peptide Symposium   2009年3月

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    記述言語:英語  

    Development of Gold Nanorods That Accumulatate into Tumor

  • 温度応答性高分子の分子設計

    森 健

    機能材料   2006年10月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 温度応答性高分子を基盤にした蛍光性温度センサ

    森 健

    ぶんせき   2004年6月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • 遺伝子DNAと高分子化学

    前田瑞夫, 森 健

    高分子   2001年4月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

  • DNA/合成高分子コンジュゲートの新機能

    森 健, 前田瑞夫

    日本油化学会誌   2000年5月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)  

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産業財産権

特許権   出願件数: 9件   登録件数: 5件
実用新案権   出願件数: 0件   登録件数: 0件
意匠権   出願件数: 0件   登録件数: 0件
商標権   出願件数: 0件   登録件数: 0件

所属学協会

  • 日本化学会

  • アメリカ化学会

  • 高分子学会

  • 分子生物学会

  • 日本DDS学会

  • バイオマテリアル学会

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委員歴

  • 分析化学会九州支部   幹事   国内

    2018年4月 - 2022年3月   

  • 高分子学会   幹事   国内

    2018年4月 - 2020年3月   

  • 高分子学会   代議員   国内

    2018年4月 - 2020年3月   

  • 高分子学会   幹事   国内

    2012年5月 - 2014年5月   

  • 高分子学会   九州支部会計   国内

    2012年5月 - 2014年5月   

  • 日本分析化学会   幹事   国内

    2011年4月 - 2012年3月   

  • 日本分析化学会   九州支部会計   国内

    2011年4月 - 2012年3月   

▼全件表示

学術貢献活動

  • シンポジウムオーガナイザー

    高分子学会討論会  ( 新潟 ) 2024年9月

     詳細を見る

    種別:大会・シンポジウム等 

  • 実行委員、シンポジウムオーガナイザー

    DDS学会  ( つくば ) 2024年7月

     詳細を見る

    種別:大会・シンポジウム等 

  • 主催

    第 3 回「物質共生」領域会議  ( 福岡 ) 2023年5月

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    種別:大会・シンポジウム等 

    参加者数:80

  • 世話人 国際学術貢献

    1 st Material Symbiosis International symposium  ( 札幌 ) 2023年3月

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    種別:大会・シンポジウム等 

    参加者数:80

  • 学術論文等の審査

    役割:査読

    2023年

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    種別:査読等 

    外国語雑誌 査読論文数:6

    日本語雑誌 査読論文数:5

  • 学術論文等の審査

    役割:査読

    2022年

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    種別:査読等 

    外国語雑誌 査読論文数:2

    日本語雑誌 査読論文数:3

  • 世話人

    日本DDS学会シンポジウム  ( 千葉 ) 2021年6月

     詳細を見る

    種別:大会・シンポジウム等 

  • 学術論文等の審査

    役割:査読

    2021年

     詳細を見る

    種別:査読等 

    外国語雑誌 査読論文数:7

  • 世話人

    分析化学会九州支部春の講演会  ( Japan ) 2020年6月

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    種別:大会・シンポジウム等 

    参加者数:50

  • 世話人

    高分子学会九州支部フォーラム  ( Japan ) 2020年1月

     詳細を見る

    種別:大会・シンポジウム等 

    参加者数:50

  • 学術論文等の審査

    役割:査読

    2020年

     詳細を見る

    種別:査読等 

    外国語雑誌 査読論文数:9

  • 学術論文等の審査

    役割:査読

    2019年

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    種別:査読等 

    外国語雑誌 査読論文数:12

  • 学術論文等の審査

    役割:査読

    2018年

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    種別:査読等 

    外国語雑誌 査読論文数:9

  • 学術論文等の審査

    役割:査読

    2017年

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    種別:査読等 

    外国語雑誌 査読論文数:12

  • 世話人

    分析化学会九州支部夏の学校  ( 北九州市 ) 2014年7月 - 2015年7月

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    種別:大会・シンポジウム等 

    参加者数:150

▼全件表示

共同研究・競争的資金等の研究課題

  • 経口投与ナノ粒子製剤

    2021年4月 - 2022年9月

    共同研究

      詳細を見る

    担当区分:研究分担者  資金種別:その他産学連携による資金

  • 抗体医薬を真に置き換える小タンパク質医薬の開発

    研究課題/領域番号:21H02061  2021年 - 2024年

    日本学術振興会  科学研究費助成事業  基盤研究(B)

      詳細を見る

    担当区分:研究代表者  資金種別:科研費

  • 膜タンパク質のノックダウンを可能にする新しい創薬概念の提案

    研究課題/領域番号:21K19054  2021年 - 2023年

    日本学術振興会  科学研究費助成事業  挑戦的研究(萌芽)

      詳細を見る

    担当区分:研究代表者  資金種別:科研費

  • 超高感度細胞膜抗原検出法の開発とがんコンパニオン診断への応用

    研究課題/領域番号:21H04686  2021年 - 2023年

    日本学術振興会  科学研究費助成事業  基盤研究(A)

      詳細を見る

    担当区分:研究分担者  資金種別:科研費

  • 抗体医薬の問題を解決し、これを代替する新しい医薬の開発

    2021年 - 2023年

    キャノン財団

      詳細を見る

    担当区分:研究代表者  資金種別:受託研究

  • 腸内共生系における弱い相互作用の理解と材料共生への応用

    研究課題/領域番号:20H05876  2020年 - 2024年

    日本学術振興会・文部科学省  科学研究費助成事業  学術変革領域研究(A)

      詳細を見る

    担当区分:研究代表者  資金種別:科研費

  • マテリアル・シンバイオシスのための生命物理化学

    研究課題/領域番号:20H05871  2020年 - 2024年

    日本学術振興会・文部科学省  科学研究費助成事業  学術変革領域研究(A)

      詳細を見る

    担当区分:研究分担者  資金種別:科研費

  • 子宮がんでのセンチネルリンパ 節同定における新規トレーサー の開発

    2019年4月 - 2022年3月

      詳細を見る

    担当区分:研究分担者 

  • 子宮体癌がん幹細胞関連蛋白SP ARCの機能解析とそれを標的と した新規薬剤の開発

    2019年4月 - 2021年3月

      詳細を見る

    担当区分:研究分担者 

  • 抗体ー糖鎖高分子複合体の創製 による細胞免疫操作法の確立

    2019年4月 - 2021年3月

      詳細を見る

    担当区分:研究分担者 

  • 子宮がんでのセンチネルリンパ節同定における新規トレーサーの開発

    研究課題/領域番号:19K09804  2019年 - 2021年

    日本学術振興会  科学研究費助成事業  基盤研究(C)

      詳細を見る

    担当区分:研究分担者  資金種別:科研費

  • 子宮体癌がん幹細胞関連蛋白SPARCの機能解析とそれを標的とした新規薬剤の開発

    研究課題/領域番号:19H03800  2019年 - 2021年

    日本学術振興会  科学研究費助成事業  基盤研究(C)

      詳細を見る

    資金種別:科研費

  • 抗体ー糖鎖高分子複合体の創製による細胞免疫操作法の確立

    研究課題/領域番号:19K22971  2019年 - 2020年

    日本学術振興会  科学研究費助成事業  挑戦的研究(萌芽)

      詳細を見る

    担当区分:研究分担者  資金種別:科研費

  • 子宮体癌がん幹細胞関連蛋白SPARCの機能解析とそれを標的とした新規薬剤の開発

    研究課題/領域番号:19H03800  2019年 - 2016年

    日本学術振興会  科学研究費助成事業  基盤研究(B)

      詳細を見る

    担当区分:研究分担者  資金種別:科研費

  • 超高感度細胞膜抗原検出法とが んコンパニオン診断への適用

    2018年4月 - 2021年3月

      詳細を見る

    担当区分:研究分担者 

  • 超高感度細胞膜抗原検出法とがんコンパニオン診断への適用

    研究課題/領域番号:18H03936  2018年 - 2020年

    日本学術振興会  科学研究費助成事業  基盤研究(A)

      詳細を見る

    担当区分:研究分担者  資金種別:科研費

  • 腸内細菌叢を保護する抗生物質の経口投与法の開発

    2017年4月 - 2019年3月

      詳細を見る

    担当区分:研究代表者 

  • 膜貫通型人工受容体の化学修飾法の開発とがん免疫治療への適用

    2017年4月 - 2019年3月

      詳細を見る

    担当区分:研究代表者 

  • 膜貫通型人工受容体の化学修飾法の開発とがん免疫治療への適用

    2017年 - 2018年

    日本学術振興会  科学研究費助成事業  挑戦的研究(萌芽)

      詳細を見る

    担当区分:研究代表者  資金種別:科研費

  • 腸内細菌叢を保護する抗生物質の経口投与法の開発

    2017年 - 2018年

    工学研究新分野開拓助成

      詳細を見る

    担当区分:研究代表者  資金種別:学内資金・基金等

  • フローサイトメトリーの高感度化のための分子技術と高精度細胞診断の実現

    2016年4月 - 2017年5月

      詳細を見る

    担当区分:研究代表者 

  • フローサイトメトリーの高感度化のための分子技術と高精度細胞診断の実現

    研究課題/領域番号:16H04167  2016年 - 2019年

    日本学術振興会  科学研究費助成事業  基盤研究(B)

      詳細を見る

    担当区分:研究代表者  資金種別:科研費

  • リポソーム型細胞架橋剤:迅速セルソーティング法と機能化自在マトリックスの創製

    2014年4月 - 2016年3月

      詳細を見る

    担当区分:研究代表者 

  • 網羅的解析の可能なペプチドアレイの開発

    2014年4月 - 2015年3月

    受託研究

      詳細を見る

    担当区分:研究分担者  資金種別:その他産学連携による資金

  • プロテインアレイ

    2014年4月 - 2015年3月

    共同研究

      詳細を見る

    担当区分:研究分担者  資金種別:その他産学連携による資金

  • リポソーム型細胞架橋剤:迅速セルソーティング法と機能化自在マトリックスの創製

    研究課題/領域番号:26620136  2014年 - 2015年

    科学研究費助成事業  挑戦的萌芽研究

      詳細を見る

    担当区分:研究代表者  資金種別:科研費

  • 病態機能イメージングのための細胞内キナーゼ活性蛍光プローブの開発

    研究課題/領域番号:25560202  2013年 - 2015年

    日本学術振興会  科学研究費助成事業  基盤研究(C)

      詳細を見る

    資金種別:科研費

  • 抗がん剤投薬前診断のための次世代ペプチドアレイ

    2012年 - 2014年

    日本学術振興会  科学研究費助成事業  基盤研究(A)

      詳細を見る

    担当区分:研究分担者  資金種別:科研費

  • 次世代タンパク質用デリバリー素材:タンパク質を温和に保持し放出するナノマシン

    研究課題/領域番号:23700539  2011年 - 2013年

    科学研究費助成事業  若手研究(B)

      詳細を見る

    担当区分:研究代表者  資金種別:科研費

  • プロテインキナーゼ応答性蛍光プローブを用いたがん診断

    2010年4月 - 2011年5月

    共同研究

      詳細を見る

    担当区分:研究分担者  資金種別:その他産学連携による資金

  • ポリ(N,N’-アルキルメチレンマロンアミド)の効率的合成とバイオメディカル応用

    2008年7月 - 2009年3月

    受託研究

      詳細を見る

    担当区分:研究代表者  資金種別:その他産学連携による資金

  • 新規温度応答性高分子のバイオメディカル応用

    2007年4月 - 2008年3月

    共同研究

      詳細を見る

    担当区分:研究代表者  資金種別:その他産学連携による資金

  • 金ナノ粒子を用いたラベルフリー細胞内シグナル迅速アッセイ法

    研究課題/領域番号:19021035  2007年 - 2008年

    日本学術振興会  科学研究費助成事業  基盤研究(C)

      詳細を見る

    資金種別:科研費

  • 血管閉塞剤としての生理温度硬化性樹脂の開発

    2007年 - 2008年

    九州地域戦略産業イノベーション創出事業

      詳細を見る

    担当区分:研究代表者  資金種別:受託研究

  • レーザー操作による有機・バイオ集積体のオンデマンド構築技術の開発

    2007年 - 2008年

    九州大学教育研究プログラム・研究拠点形成プロジェクト申請書

      詳細を見る

    担当区分:研究分担者  資金種別:学内資金・基金等

  • サブナノリットルを操作容量とするキナーゼ検出用ペプチドアレイの開発

    2007年

    新世代研究所研究助成

      詳細を見る

    担当区分:研究代表者  資金種別:受託研究

  • 貴金属ナノ粒子を用いた高感度かつハイスループットな薬剤探索法およびがん診断法の開発

    2007年

    貴金属に関する研究助成金制度

      詳細を見る

    担当区分:研究代表者  資金種別:受託研究

  • ラジカル重合反応を用いるたんぱく質β-シートの高機能化

    2006年4月 - 2008年3月

      詳細を見る

    担当区分:研究代表者 

  • 新規温度応答性高分子の開発

    2006年4月 - 2007年3月

    共同研究

      詳細を見る

    担当区分:研究代表者  資金種別:その他産学連携による資金

  • ハイパーブランチポリマーを利用したペプチドアレイの表面改質

    2006年4月 - 2007年3月

      詳細を見る

    担当区分:研究分担者  資金種別:その他産学連携による資金

  • 金属表面修飾によるマイクロアレイセンサの高感度化

    2006年4月 - 2007年3月

    共同研究

      詳細を見る

    担当区分:研究分担者  資金種別:その他産学連携による資金

  • ボトムアップによるナノスケール材料の合成とその電気的および光学的デバイスへの応用

    2003年4月 - 2004年3月

      詳細を見る

    担当区分:研究代表者 

  • 分子レベルで編まれたシート状ポリマーの開発

    2003年4月 - 2004年3月

      詳細を見る

    担当区分:研究代表者 

  • ロッド−コイル型グラフトポリマーの自己組織化による高導電性ナノワイヤーの調製

    2003年4月 - 2004年3月

      詳細を見る

    担当区分:研究分担者 

  • 分子レベルで編まれたシート状ポリマーの開発

    2003年

    財団法人日本科学協会

      詳細を見る

    担当区分:研究代表者  資金種別:受託研究

  • ロッド−コイル型グラフトポリマーの自己組織化による高導電性ナノワイヤーの調製

    2003年

    財団法人新世代研究所

      詳細を見る

    担当区分:研究代表者  資金種別:受託研究

  • ボトムアップによるナノスケール材料の合成とその電気的および光学的デバイスへの応用

    2003年

    工学部研究プロジェクト(徳島大学)

      詳細を見る

    担当区分:研究代表者  資金種別:学内資金・基金等

  • 自己組織化ブロックポリマーを用いた被覆型導電性ナノワイヤーの開発

    2002年4月 - 2005年3月

      詳細を見る

    担当区分:研究分担者 

  • 導電性高分子のひも状集合体を化学架橋により固定化・被覆したナノ導線の開発

    2002年4月 - 2005年3月

      詳細を見る

    担当区分:研究代表者 

  • 感熱性ポリマー・DNA複合体の開発と遺伝子診断ならびにDDSへの応用

    研究課題/領域番号:99J03315  1999年 - 2000年

    日本学術振興会  科学研究費助成事業  基盤研究(C)

      詳細を見る

    資金種別:科研費

▼全件表示

担当授業科目

  • 生化学第二

    2023年4月 - 2023年9月   前期

  • 医用化学第一

    2022年4月 - 2022年9月   前期

  • 生化学第二

    2022年4月 - 2022年9月   前期

  • 医用化学基礎

    2022年4月 - 2022年9月   前期

  • 生化学第二

    2021年6月 - 2021年8月   夏学期

  • 分子生物学

    2021年4月 - 2021年9月   前期

  • 医用化学基礎

    2020年4月 - 2020年9月   前期

  • 医用化学第一

    2020年4月 - 2020年9月   前期

  • 生化学第二

    2020年4月 - 2020年9月   前期

  • 分子生物学

    2020年4月 - 2020年9月   前期

  • 生化学第二

    2019年4月 - 2019年9月   前期

  • 分子生物学

    2019年4月 - 2019年9月   前期

  • 医用化学第一

    2018年10月 - 2019年3月   後期

  • 生化学第二

    2018年4月 - 2018年9月   前期

  • 分子生物学

    2018年4月 - 2018年9月   前期

  • 医用化学基礎

    2018年4月 - 2018年9月   前期

  • 生化学第二

    2017年4月 - 2017年9月   前期

  • 機能性高分子化学

    2017年4月 - 2017年9月   前期

  • 物質科学学生セミナー第二

    2016年4月 - 2017年3月   通年

  • 物質科学コミュニケーション第二

    2016年4月 - 2016年9月   前期

  • 物質科学学生セミナー第二

    2016年4月 - 2016年9月   前期

  • 生体分子有機化学

    2015年4月 - 2015年9月   前期

  • 課題協学

    2014年10月 - 2015年3月   後期

  • 生体分子有機化学

    2014年10月 - 2015年3月   後期

  • 物質科学学生セミナー第二

    2014年4月 - 2015年3月   通年

  • 物質科学コミュニケーション第二

    2014年4月 - 2015年3月   通年

  • 生体高分子化学特論

    2014年4月 - 2014年9月   前期

  • 物質科学コミュニケーション第二

    2013年4月 - 2014年3月   通年

  • 物質科学学生セミナー第二

    2013年4月 - 2014年3月   通年

  • 物質科学学生セミナー第二

    2012年4月 - 2013年3月   通年

  • 物質科学コミュニケーション第二

    2012年4月 - 2013年3月   通年

  • 物質科学学生セミナー第二

    2011年4月 - 2012年3月   通年

  • 物質科学コミュニケーション第二

    2011年4月 - 2012年3月   通年

  • 物質科学コミュニケーション第二

    2010年4月 - 2011年3月   通年

  • 物質科学セミナー第二

    2010年4月 - 2011年3月   通年

  • 物質科学セミナー第二

    2009年4月 - 2010年3月   通年

  • 物質科学コミュニケーション第二

    2009年4月 - 2010年3月   通年

  • 物質科学学生セミナー第二

    2008年4月 - 2009年3月   通年

  • 物質科学コミュニケーション第二

    2008年4月 - 2009年3月   通年

  • 材料物性工学演習−A

    2005年4月 - 2006年3月   通年

▼全件表示

社会貢献活動

  • 医療に貢献する化学~工学部化学系の医薬研究~

    長崎県立諫早高等学校  2021年7月

     詳細を見る

    対象:幼稚園以下, 小学生, 中学生, 高校生

    種別:セミナー・ワークショップ

  • 医療に貢献する化学

    広島市立基町高等学校  2014年10月

     詳細を見る

    対象:幼稚園以下, 小学生, 中学生, 高校生

    種別:セミナー・ワークショップ

メディア報道

  • 花粉治療 副作用軽く 新聞・雑誌

    読売新聞  2023年2月

     詳細を見る

    花粉治療 副作用軽く

  • アレルギー治療向け微粒子 新聞・雑誌

    日経新聞  2023年2月

     詳細を見る

    アレルギー治療向け微粒子

  • ヒステリシスが17度C ビニルポリマー系熱応答性高分子を開発 新聞・雑誌

    化学工業日報  2006年4月

     詳細を見る

    ヒステリシスが17度C ビニルポリマー系熱応答性高分子を開発

外国人研究者等の受け入れ状況

  • マインツ大学

    受入れ期間: 2023年10月 - 2024年2月  

    国籍:日本国

  • ポリテクニーク・ド・ボルドー

    受入れ期間: 2023年4月 - 2023年9月   (期間):1ヶ月以上

    国籍:フランス共和国

    専業主体:文部科学省

  • マインツ大学

    受入れ期間: 2022年10月 - 2023年3月   (期間):1ヶ月以上

    国籍:ドイツ連邦共和国

    専業主体:文部科学省

  • Liaoning Research Institute of Family Planning

    受入れ期間: 2018年5月 - 2018年11月   (期間):1ヶ月以上

    国籍:中華人民共和国

    専業主体:民間・財団

海外渡航歴

  • 2023年7月

    滞在国名1:中華人民共和国   滞在機関名1:四川大学

学内運営に関わる各種委員・役職等

  • 2021年10月 - 2023年9月   部門 国際コースカリキュラム委員

  • 2020年4月 - 2022年3月   部門 環境保全委員

  • 2019年10月 - 2021年9月   部門 国際コース入試委員

  • 2018年10月 - 2020年9月   研究院 国際コース委員、運営委員

  • 2016年4月 - 2017年5月   部門 学務補佐

  • 2016年4月 - 2017年3月   部門 教育企画委員

  • 2015年4月 - 2017年3月   部門 カリキュラム

  • 2015年4月 - 2016年3月   部門 G-30入試委員

  • 2014年4月 - 2016年3月   部門 分析センター工学分室 委員

  • 2014年3月 - 2016年4月   部門 広報委員会(工学研究院)

  • 2013年4月 - 2014年3月   部門 情報図書委員

  • 2006年4月 - 現在   部門 消防委員

  • 2006年4月 - 現在   部門 防災委員

▼全件表示