Language：English   Publisher：Biotechnology Letters  

Objectives: During physical transfection, an electrical field or mechanical force is used to induce cell transfection. We tested if the disruption of a dense actin layer underneath the membrane of a suspended cell enhances cell transfection. Results: A bubble generator was used to electromechanically stimulate suspended cells. To clarify the influence of the actin layer (the actin cortex) on cell transfection efficiency, we used an actin polymerization inhibitor (cytochalasin D) to disrupt the actin cortex before electromechanical stimulation. Without cytochalasin D treatment, signals from the overall actin cortex decreased after electromechanical stimulation. With cytochalasin D treatment, there was localized F-actin aggregation under static conditions. After electromechanical stimulation, there was a partial loss (localized disruption), but no overall disruption, of the actin cortex. With the pretreatment with cytochalasin D, the transfection efficiency of plasmids (4.7, 8.3, or 11 kbp) into NIH/3T3 or UMR-106 cells increased significantly after exposure to electromechanical stimulation. Conclusions: Localized distribution of the actin cortex before exposure to electromechanical stimulation is crucial for inducing a partial loss of the cortex, which improves transfection efficiency and large plasmid delivery.

DOI： 10.1007/s10529-023-03382-y

Web of Science

Scopus

PubMed

Language：Japanese   Publisher：The Japan Society for Precision Engineering  

DOI： 10.2493/jjspe.89.669

Scopus

CiNii Research

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1021/acsnano.3c07461

Web of Science

Scopus

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

Publisher：2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023  

We propose a microfluidic device for continuous production of fused cells by encapsulating cells in microdroplets for paring, separating them from empty ones, and applying voltage to them (Fig. 1). This system is expected to overcome potential issues of conventional microfluidic methods using microstructures for cell pairing, which are limitation in size and number of target cells, and difficulty in collecting fused cells. Here, we demonstrate cell fusion by applying pulse voltage to droplets in which two cells are packed tightly and confirmed the presence of some fused cells in collected cell-encapsulated droplets.

Scopus

Language：Japanese   Publisher：The Japan Society of Mechanical Engineers  

<p>On-chip mechano-indexing has many advantages such as low risk of contamination, stable environment for manipulation, and flow cytometric measurement. In this paper, we propose a microfluidic chip made by rigid polymers; two cyclo-olefin polymer substrates and a polyimide film with force sensor probes. Compared to the conventional robot integrated microfluidic chip composed of glass – silicon – glass layers, the proposed COP – PI – COP chip allow us to reduce the fabrication process with keeping rigidity for the sensor probe. In the experiment, we succeeded in driving a force sensor probe with the fabricated microfluidic chip and measuring the mechano-index of a hydrogel.</p>

DOI： 10.1299/jsmermd.2023.2a1-f10

CiNii Research

Language：English   Publisher：一般社団法人 日本フルードパワーシステム学会  

DOI： 10.14888/jjfpss.54.3_121

CiNii Research

Publisher：2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023  

We propose a novel microfluidic technique for the high-efficient encapsulation of double cells into a droplet. For double-cells encapsulation into a droplet, we designed a microfluidic droplet generator (MFDG), consisting of the rhombic-shaped reservoir channel and constriction channel. The designed MFDG acts as a "virtual particles valve"because of the trapping of the single cell in the reservoir channel and releasing the cells when another cell flow into there. By tuning the flow rates for dispersed and continuous phases, we found that the double-cells encapsulation efficiency could be increased compared to the theoretical encapsulation efficiency derived by the Poisson distribution.

Scopus

Publisher：Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)  

In this paper, we proposed a novel needle-free injection method using electrically induced microbubbles. This microbubble needle-free injector is developed to achieve the depth-controlled drug injection. A focusing device was developed to enhance the pressure of microbubble-induced shock wave and improve the perforation ability of injector. Due to the injection depth being related to the distance between injector tip and target, we propose to control the injection depth by sensing and adjusting the distance. We developed the distance measurement by microbubble injector itself. In this work, the microbubble injector was both the actuator and sensor. This needle-free microbubble is a promising tool to achieve the injection depth-controlled in the future.

DOI： 10.1109/MEMS49605.2023.10052502

Web of Science

Scopus

Publisher：Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)  

We propose a local metal deposition method that enables patterning on hydrogels using micro-plasma-bubbles (MPBs). In this method, we generate MPBs in a solution containing metal ions. Local metal deposition on hydrogels has a wide range of possibilities, however, it has been a considerable challenge since appropriate methods have not been established. In this work, we reduced metal ions in an electrolyte solution and successfully deposited several kinds of metals such as copper, nickel, copper-nickel alloy, and even gold nanoparticles on hydrogels by using MPBs. These results show that the proposed method opens up new possibilities of an on-demand metallization process for hydrogels that can serve as soft actuators and electronics.

DOI： 10.1109/MEMS49605.2023.10052218

Web of Science

Scopus

Publisher：2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023  

We propose a novel droplet microfluidic device for high-throughput generation of giant liposomes without surface modification of microchannel by utilizing the step emulsification and the parallelized droplet transfer microchannels. Unlike the conventional microfluidic techniques for production of giant liposomes, limiting the throughput due to the low production rate of droplets in single microfluidic droplet generator, the proposed device could produce giant liposomes with high throughput manner. Our microfluidic device consists of two main parts; (i) water-in-oil (W/O) droplet generation through 100 parallelized micro nozzles, and (ii) generation of uniform W/O/W droplet through parallelized droplet transfer channels. After generation of W/O/W droplets at 332 Hz, they transformed into giant liposome by dewtting process.

Scopus

Publisher：2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023  

We present a high-speed on-chip liquid dispensing by utilizing on-demand vortex generations for analyzing dynamic cellular functions against osmotic change. Unlike previous methods, our system can discretely dispense the liquid in laminar flow by employing traveling vortex containing different solutions from normal medium. Therefore, we can switch microscale environment surrounding single cells with high-speed of under 2.25 ms. Moreover, we can repeatedly switch the environment with the high-frequency of approximately 400 Hz, which is a unique technique to investigate the frequency response of cells against environmental change. In this paper, we demonstrated repetitive stimulations to a single micro-alga. Our technique will contribute to unexplored high-speed and frequency cellular dynamic responses.

Scopus

Publisher：2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023  

We present on-chip multi-sorting of single cells by utilizing high-speed flow control with high-resolution. We utilized dual-membrane pumps integrated into a microfluidic chip, which were driven by the piezoelectric actuators. We evaluated the performance of multi-sorting using multi-threshold for fluorescent intensity. As results, we achieved high-speed sorting with the response time of 86 μs (equivalent to 8.6 kHz). We demonstrated on-chip multi-sorting using microbeads and EGFP-expressing cell, and they were successfully sorted into 6 groups based on five thresholds. The presented multi-sorting opens new paradigms of detailed analyses of bioparticles.

Scopus

Publisher：2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023  

In this research, we proposed a novel non-contact distance sensor using electrically induced microbubble. The microbubble generated from sensor tip by applying the voltage pulse to the sensor. The microbubble expanded and reached the object at a certain time. A sudden change in the current flowing through the sensor occurred when microbubble reached the object. This distance sensing method was developed by building a relation between the current and the distance. In this paper, electrically induced microbubble generation mechanism, current characteristics of microbubble touching different material target, distance-current characteristics development and the improvement of distance measurement range was introduced.

Scopus

Publisher：2023 22nd International Conference on Solid-State Sensors, Actuators and Microsystems, Transducers 2023  

We propose a novel generation technique of DNA-functionalized colloidal crystals, which have unique functional properties (e.g. encapsulation of molecules), inside a giant unilamellar vesicle (GUV) toward the controlling the numbers of crystals introduced into the cells by cell-GUV membrane fusion. We designed and fabricated a microfluidic device to generate monodisperse water-in-oil-in-water (W/O/W) droplets containing the DNA-functionalized nanoparticle (DNA-NP), which is a precursor of GUV, for generation of GUV encapsulating a single crystal. We demonstrate production of W/O/W droplets encapsulating DNA-NP, collection of these droplets into microtubes, and the single crystallization of DNA-NP inside the produced W/O/W droplet through annealing process in the incubator.

Scopus

Language：English  

DOI： 10.1038/s41540-022-00255-4

Web of Science

PubMed

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Royal Society of Chemistry (RSC)  

Cell poration technologies offer opportunities not only to understand the activities of biological molecules but also to investigate genetic manipulation possibilities. Unfortunately, transferring large molecules that can carry huge genomic information is challenging. Here, we demonstrate electromechanical poration using a core–shell-structured microbubble generator, consisting of a fine microelectrode covered with a dielectric material. By introducing a microcavity at its tip, we could concentrate the electrical field with the application of electric pulses and generate microbubbles for electromechanical stimulation of cells. Specifically, the technology enables transfection with molecules that are thousands of kDa even into osteoblasts and Chlamydomonas, which are generally considered to be difficult to inject. Notably, we found that the transfection efficiency can be enhanced by adjusting the viscosity of the cell suspension, which was presumably achieved by remodeling of the membrane cytoskeleton. The applicability of the approach to a variety of cell types opens up numerous emerging gene engineering applications.

DOI： 10.1039/d2lc00628f

Web of Science

Scopus

PubMed

CiNii Research

researchmap

Language：English   Publishing type：Research paper (scientific journal)  

DOI： https://doi.org/10.1039/d2lc00628f

Repository Public URL： https://hdl.handle.net/2324/7167119

Language：English   Publishing type：Research paper (scientific journal)  

DOI： https://doi.org/10.3390/mi13081312

Language：English   Publisher：Micromachines  

3D wiring technology is required for the integration of micro–nano devices on various 3D surfaces. However, current wiring technologies cannot be adapted to a variety of materials and surfaces. Here, we propose a new metal deposition method using only a micro-plasma bubble injector and a metal ion solution. Micro-plasma bubbles were generated on demand using pulses, and the localized reaction field enables metal deposition independent of the substrate. Three different modes of micro-plasma bubble generation were created depending on the power supply conditions and mode suitable for metal deposition. Furthermore, using a mode in which one bubble was generated for all pulses among the three modes, copper deposition on dry/wet materials, such as chicken tissue and glass substrates, was achieved. In addition, metal deposition of copper, nickel, chromium, cobalt, and zinc was achieved by simply changing the metal ion solution. Finally, patterning on glass and epoxy resin was performed. Notably, the proposed metal deposition method is conductivity independent. The proposed method is a starting point for 3D wiring of wet materials, which is difficult with existing technologies. Our complete system makes it possible to directly attach sensors and actuators to living organisms and robots, for example, and contribute to soft robotics and biomimetics.

DOI： 10.3390/mi13081312

Web of Science

Scopus

PubMed

Publisher：MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences  

We present a large particles ordering technique for controlling event intervals by high-speed flow control. Unlike conventional ordering methods targeting small particles less than 50 μm, our method can regulate event intervals of larger particles around 100 μm by the combinations of concentration and spacing mechanisms. Specifically, we employed dual vortices for spacing which work as virtual gate to stop and transport particles by vortices generations cycles. As results, we successfully ordered 100-μm beads with the intervals of multiple of 2 ms. Thus, our method not only overcome the random distributions but also open up new microfluidic applications for large particles.

Scopus

Publisher：Proceedings - IEEE International Conference on Robotics and Automation  

Cell fusion has been widely applied in scientific research for cancer immunotherapy, antibody production, and nuclear reprogramming of somatic cells, and therefore the cell fusion technique that enable us to precisely control the fusion process with high throughput manner has been desired. Here, we present a novel microfluidic method for automatic cell pairing by microdroplets, separation of droplets containing cells, and electrofusion of cells inside a droplet. The proposed microfluidic device mainly composed of three sequential function parts for (i) encapsulation of cells into a droplet by microfluidic droplet generator, (ii) separation of droplets containing cells from empty droplets through a micropillar array, and (iii) electrofusion of cells inside the droplets by applying a voltage during the droplet passing over the pair of electrodes. In the microfluidic device, cell-encapsulated and empty droplets were generated at the upstream cross-junction; they then entered the micropillar array, separating the cell-encapsulated droplets from empty droplets continuously. After separation, they passed over the electrode pairs, and were collected the outside of the microchannel. This continuous process for cell fusion would enable us to observe and isolate the target fused cells for cell analysis.

DOI： 10.1109/ICRA46639.2022.9812390

Web of Science

Scopus

Publisher：2022 IEEE International Conference on Cyborg and Bionic Systems, CBS 2022  

Plasma generated in liquids has attracted attention as a high-speed reaction field in the fields of surface modification, material synthesis, and water purification. In particular, electrically generated plasma is used for the synthesis of metal/metal oxides by inducing electrochemical reactions through the plasma. We have previously proposed a metal deposition method using plasma-induced bubbles, which can be generated on demand by applying a voltage pulse. However, the mechanism of their generation has not been clarified, and a design theory for device design has not been established. Here, we discuss a method for the on-demand generation of microplasma bubbles by electric field concentration. In this paper, we aimed to construct a finite element analysis model for device design. The validity of the analytical model was confirmed by comparison with experiments. Specifically, we focused on the location of bubble generation, response speed, and density change. As a result, the analytical model was able to reproduce the position of bubble generation, response speed, and density change during pulse application. It was also found that under most conditions, the bubble generation was due to heat. Therefore, by using a model that considers only heat, it is possible to construct a design model with a low computational cost.

DOI： 10.1109/CBS55922.2023.10115333

Web of Science

Scopus

Publisher：MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences  

Delivering nanoartifacts such as molecules and nanosensors into cells is a fundamental technology contributing to not only gene editing for obtaining desired cells but also measurement of intracellular environment. A method enabling to locally deliver molecules into targeted cells under culture condition is coveted, however, existing technologies represented by electroporation are hard to offer such opportunities. Here, we present an in-situ injection system based on local electroporation by a robotic-controlled microelectrode. We demonstrated two diverse applications: 2D drawing using plasmid DNA (4.7 kbp) and FITC-labeled dextran (250 kDa and 2 MDa), and in-situ injection of ribonucleoprotein (guide RNA with Cas9).

Scopus

Publisher：MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences  

We propose a novel droplet microfluidic device for high-throughput generation of giant unilamellar vesicles (GUVs) through dewetting process of the water-in-oil-in-water (W/O/W) double emulsions, which generated by step emulsification and droplet transfer technique. Our microfluidic device consists of two main functional parts; water-in-oil (W/O) droplet generation through 300 parallelized micro nozzles, and generation of W/O/W double emulsions by transferring the produced W/O droplets across the oil-water interface. We demonstrated the W/O droplet generation in step emulsification channels followed by the formation of W/O/W double emulsions (~500 droplets/s) by transferring across the oil-water interface for production of GUVs.

Scopus

Publisher：MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences  

We propose high-speed on-chip flow control technique utilizing COP (cyclo-olefin polymer) membrane pump. Among the substrate material of microfluidic chip, COP provides advantages of transparency, biocompatibility and ease of fabrication. To extend the functionality of this superior COP chip, we develop COP membrane pump which can follow the high-speed actuation of the piezoelectric actuator by using its sufficient rigidity. As examples of functionalities, we demonstrated the on-chip sorting with the throughput of 3.6 kHz, and the on-chip mixing in the order of millisecond. These results indicate not only capability of high-speed flow control but also broad applicability of our method.

Scopus

Publisher：2022 IEEE International Conference on Cyborg and Bionic Systems, CBS 2022  

We have developed a novel needle-free injector based on the electrically induced microbubbles. In this article, firstly we evaluated the reagent injection depth using electrically induced microbubble injector. Then, we created elliptical hemispherical reflectors to focus the shock waves from the microbubbles and thus amplify the shock and expansion waves. We observed the shock and expansion waves from the microbubble by the ripple shadowing method and realized the amplification of the shock and expansion waves by the fabricated reflector.

DOI： 10.1109/CBS55922.2023.10115380

Web of Science

Scopus

Publisher：MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences  

We present versatile metal deposition on hydrogels utilizing micro-plasma-bubbles (MPBs), or local plasma reaction fields in solution. Although metallization of such soft and wet materials has been a tough challenge, we succeeded in precipitating metal on the surface of hydrogel by reducing metal ions. Our method is applicable to many kinds of metal species. We confirmed copper, nickel, and cobalt deposition on hydrogels. Even alloy was successfully deposited just by mixing the solutions before generating MPB. Then we demonstrate that deposited nickel has a magnetic property. Results showed that we developed an on-demand hydrogel metallization system with considerable versatility.

Scopus

Publisher：MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences  

We propose a novel microfluidic system utilizing microdroplets for continuous cell pairing, fusion, and collection of the fused cells to the outside. Unlike the conventional microfluidic cell fusion using microstructures, which restrict the cell sizes and the number of cells for pairing, and the collection to the outside, our microfluidic device utilize droplets as the field of cell fusion, enabling the continuous cell pairing, fusion, and collection of the fused cells. We demonstrated cell fusion in droplets by tightly packing the cells into droplets for pairing, separation of cell-encapsulated droplets, and applying the pulsed-voltage to the droplets.

Scopus

Publisher：MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences  

Nanofunctioners represented by nanosensors and nucleic acids offer major leap forwards in understanding intercellular communication by not only giving triggers to start talking but also visualizing the ways to transfer information. However, transportation of various nanofunctioners into desired live-cells is still challenging. We present a revolutionary concept, “avatar cells”, which are living cells gifted nanofunctioners as if living carriers. Two-types of avatar cells based on neutrophils and macrophages were produced by transporting nanofunctioners: fluorescent nanodiamond (FND) and fluorescent DNA stain contained in monodispersed lipid nanoparticles (LNPs), respectively. We demonstrated visualization of intercellular communication during NETosis triggered by byphorbol12-myristate13-acetate (PMA).

Scopus

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1073/pnas.2107461118

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1038/s41598-020-69804-3

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/s19153254

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3390/mi10060389

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1088/2399-6528/ab0c30

Language：Japanese   Publishing type：Research paper (scientific journal)  

DOI： doi.org/10.2491/jjsth.30.544

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1002/eej.23238

Language：Japanese   Publishing type：Research paper (scientific journal)  

A novel needle-free injection system by electrically induced microbubbles has been developed. First of all, we evaluated the depth of injected reagent for evaluating injection performance. Then, the cavitation-induced shock wave has been focused to magnify the injection performance by semi-spherical reflector. We successfully visualized the shock wave by Shlieren method and succeeded in measuring the pressure of focused shock wave and expansion wave by hydrophone.

DOI： 10.1541/ieejsmas.139.122

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1109/JMEMS.2018.2793314

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1246/cl.170217

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.7567/APEX.9.096201

Language：English   Publishing type：Research paper (scientific journal)  

Event date： 2016.12

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：九大医学部百年講堂   Country：Japan  

Event date： 2016.11 - 2016.12

Language：English   Presentation type：Oral presentation (general)  

Country：United States  

Event date： 2016.10

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Country：Japan  

Event date： 2016.8

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

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Venue：機械振興会館，JAPAN   Country：Japan  

Event date： 2016.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：文部科学省 科学技術・学術政策研究所 会議室   Country：Japan  

Event date： 2016.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東京ビックサイト   Country：Japan  

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Country：Japan  

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Venue：東工大大岡山キャンパス   Country：Japan  

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Venue：オンライン   Country：Japan  

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Venue：福岡   Country：Japan  

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Event date： 2023.12

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：山口   Country：Japan  

Event date： 2023.11 - 2025.11

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Event date： 2023.11 - 2025.11

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Event date： 2023.11 - 2025.11

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Event date： 2023.11

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Venue：熊本   Country：Japan  

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Venue：熊本   Country：Japan  

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Venue：熊本   Country：Japan  

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Venue：熊本   Country：Japan  

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Venue：熊本   Country：Japan  

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Venue：熊本   Country：Japan  

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Venue：熊本   Country：Japan  

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Venue：熊本   Country：Japan  

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Event date： 2023.8 - 2023.9

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Country：Japan  

Event date： 2023.8 - 2023.9

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Event date： 2023.6

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Venue：名古屋   Country：Japan  

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Venue：名古屋   Country：Japan  

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Venue：名古屋   Country：Japan  

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Venue：名古屋   Country：Japan  

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Venue：名古屋   Country：Japan  

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Venue：名古屋   Country：Japan  

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Venue：名古屋   Country：Japan  

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Venue：名古屋   Country：Japan  

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Venue：Kyoto   Country：Japan  

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Country：Japan  

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Venue：福岡   Country：Japan  

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Venue：福岡   Country：Japan  

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Venue：仙台   Country：Japan  

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Venue：札幌   Country：Japan  

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Event date： 2022.12

Language：Japanese   Presentation type：Oral presentation (general)  

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Venue：徳島   Country：Japan  

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Venue：徳島   Country：Japan  

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Venue：徳島   Country：Japan  

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Venue：徳島   Country：Japan  

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Venue：徳島   Country：Japan  

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Venue：徳島   Country：Japan  

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Venue：徳島   Country：Japan  

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Venue：徳島   Country：Japan  

Event date： 2022.10

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Country：Japan  

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Event date： 2022.10

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Country：China  

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Country：China  

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Country：Japan  

Event date： 2022.9

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2022.9

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2022.5

Language：Japanese  

Venue：東京   Country：Japan  

Event date： 2022.5

Language：Japanese  

Venue：東京   Country：Japan  

Event date： 2022.5

Language：Japanese  

Venue：東京   Country：Japan  

Event date： 2022.5

Language：Japanese  

Venue：東京   Country：Japan  

Event date： 2022.5

Language：Japanese  

Venue：東京   Country：Japan  

Event date： 2022.5

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2022.3

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2021.12

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.12

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2021.12

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2021.12

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2021.12

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2021.12

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2021.10

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.10

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.10

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2021.6 - 2022.6

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2021.6

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2021.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：金沢市   Country：Japan  

Event date： 2021.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：金沢市   Country：Japan  

Event date： 2021.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：金沢市   Country：Japan  

Event date： 2021.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2021.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Event date： 2020.6 - 2021.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：金沢市   Country：Japan  

Event date： 2019.3

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2019.2

Language：Japanese  

Country：Japan  

Event date： 2019.2

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2019.2

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2019.2

Language：Japanese  

Country：Japan  

Event date： 2018.12

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2018.12

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2018.12

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2018.11

Language：English   Presentation type：Oral presentation (general)  

Event date： 2018.11

Language：English  

Country：Taiwan, Province of China  

Event date： 2018.10

Language：Japanese  

Venue：札幌市民交流プラザ   Country：Japan  

Event date： 2018.10

Language：Japanese  

Venue：札幌市民交流プラザ   Country：Japan  

Event date： 2018.10

Language：Japanese  

Venue：札幌市民交流プラザ   Country：Japan  

Event date： 2018.10 - 2019.10

Language：Japanese  

Venue：札幌市民交流プラザ   Country：Japan  

Event date： 2018.10

Language：Japanese  

Venue：札幌市民交流プラザ   Country：Japan  

Event date： 2018.10

Language：Japanese  

Venue：札幌市民交流プラザ   Country：Japan  

Event date： 2018.10

Language：Japanese  

Venue：札幌市民交流プラザ   Country：Japan  

Event date： 2018.10

Language：Japanese  

Venue：札幌市民交流プラザ   Country：Japan  

Event date： 2018.10

Language：Japanese  

Country：Japan  

Event date： 2018.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：関西大学   Country：Japan  

Event date： 2018.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：北海道大学工学部   Country：Japan  

Event date： 2018.7

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Country：Japan  

Event date： 2018.7

Language：English   Presentation type：Oral presentation (general)  

Country：Ireland  

Event date： 2018.7

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2018.6

Language：Japanese  

Venue：北九州国際コンベンションホール   Country：Japan  

Event date： 2018.6

Language：Japanese  

Country：Japan  

Event date： 2018.6

Language：Japanese  

Venue：北九州国際コンベンションホール   Country：Japan  

Event date： 2018.6

Language：Japanese  

Venue：北九州国際コンベンションホール   Country：Japan  

Event date： 2018.6

Language：Japanese  

Venue：北九州国際コンベンションホール   Country：Japan  

Event date： 2018.6

Language：Japanese  

Venue：北九州国際コンベンションホール   Country：Japan  

Event date： 2018.6

Language：Japanese  

Venue：北九州国際コンベンションホール   Country：Japan  

Event date： 2018.5

Language：Japanese  

Venue：産総研つくばセンター   Country：Japan  

Event date： 2018.5

Language：Japanese  

Venue：産総研つくばセンター   Country：Japan  

Event date： 2018.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：九州大学伊都キャンパス   Country：Japan  

Event date： 2018.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：日本学術会議講堂   Country：Japan  

Event date： 2018.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：九州大学伊都キャンパス   Country：Japan  

Event date： 2018.3

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2018.3

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2018.3

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2018.2 - 2019.2

Language：Japanese  

Country：Japan  

Event date： 2018.1

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：北海道大学大学院工学研究科機械工学専攻   Country：Japan  

Event date： 2018.1

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2017.12

Language：Japanese  

Venue：京都大学百周年時計記念館   Country：Japan  

Event date： 2017.11

Language：Japanese  

Venue：九州大学 医学部百年講堂   Country：Japan  

Event date： 2017.10 - 2017.11

Language：Japanese  

Venue：広島   Country：Japan  

Event date： 2017.10 - 2017.11

Language：Japanese  

Venue：広島   Country：Japan  

Event date： 2017.10 - 2017.11

Language：Japanese  

Venue：広島   Country：Japan  

Event date： 2017.10

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：徳島大学理工学部共通講義   Country：Japan  

Event date： 2017.10

Language：English  

Country：United States  

Event date： 2017.10

Language：English  

Country：Japan  

Event date： 2017.10

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：九州大学西新プラザ   Country：Japan  

Event date： 2017.10

Language：Japanese  

Country：Japan  

Event date： 2017.10

Language：Japanese  

Venue：桐生市市民文化会館   Country：Japan  

Event date： 2017.10

Language：Japanese  

Venue：桐生市市民文化会館   Country：Japan  

Event date： 2017.9

Language：Japanese  

Venue：福岡国際会議場   Country：Japan  

Event date： 2017.9

Language：Japanese  

Venue：福岡国際会議場   Country：Japan  

Event date： 2017.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福岡国際会議場   Country：Japan  

Event date： 2017.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：埼玉大学   Country：Japan  

Event date： 2017.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：埼玉大学   Country：Japan  

Event date： 2017.9

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2017.9

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2017.6

Language：English   Presentation type：Oral presentation (general)  

Country：Taiwan, Province of China  

Event date： 2017.5

Language：Japanese  

Venue：東京工業大学，大岡山キャンパス   Country：Japan  

Event date： 2017.5

Language：Japanese  

Venue：東京工業大学，大岡山キャンパス   Country：Japan  

Event date： 2017.5

Language：Japanese  

Venue：ビックパレットふくしま   Country：Japan  

Event date： 2017.5

Language：Japanese  

Country：Japan  

Event date： 2017.5

Language：Japanese  

Venue：ビックパレットふくしま   Country：Japan  

Event date： 2017.5

Language：Japanese  

Venue：ビックパレットふくしま   Country：Japan  

Event date： 2017.5

Language：Japanese  

Venue：ビックパレットふくしま   Country：Japan  

Event date： 2017.5

Language：Japanese  

Venue：ビックパレットふくしま   Country：Japan  

Event date： 2017.5

Language：Japanese  

Venue：ビックパレットふくしま   Country：Japan  

Event date： 2017.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：早稲田大学   Country：Japan  

Event date： 2016.9

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2016.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：九大伊都キャンパス   Country：Japan  

Event date： 2016.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：九大伊都キャンパス   Country：Japan  

Event date： 2016.9

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2016.9

Language：Japanese  

Venue：幕張メッセ   Country：Japan  

Event date： 2016.9

Language：Japanese  

Venue：幕張メッセ   Country：Japan  

Event date： 2016.9

Language：English   Presentation type：Oral presentation (general)  

Country：Slovakia  

Event date： 2016.6

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2016.6

Language：Japanese  

Venue：横浜パシフィコ   Country：Japan  

Event date： 2016.6

Language：Japanese  

Venue：横浜パシフィコ   Country：Japan  

Event date： 2016.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：横浜パシフィコ   Country：Japan  

Event date： 2016.6

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：横浜パシフィコ   Country：Japan  

Event date： 2016.4

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東大生研   Country：Japan  

Event date： 2016.4

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東大生研   Country：Japan  

Event date： 2016.4

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2016.4

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2016.4

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2016.4

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2015.10

Language：English  

Country：Korea, Republic of  

Event date： 2015.6

Language：English  

Country：United States  

Event date： 2015.6

Language：English  

Country：United States  

Event date： 2014.11

Language：English  

Country：Japan  

Event date： 2014.11

Language：English  

Country：Japan  

Event date： 2014.10

Language：English  

Country：United States  

Event date： 2014.10

Language：English  

Country：United States  

Event date： 2013.11

Language：English  

Country：Japan  

Event date： 2013.10

Language：English  

Country：Germany  

Event date： 2013.5

Language：English  

Country：Germany  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン   Country：Japan  

Type：Academic society, research group, etc. 

Type：Competition, symposium, etc. 

Number of participants：1,500

Type：Academic society, research group, etc. 

Type：Competition, symposium, etc. 

Number of participants：500

Type：Peer review 

Number of peer-reviewed articles in foreign language journals：5

Number of peer-reviewed articles in Japanese journals：4

Type：Competition, symposium, etc. 

Number of participants：500

Type：Competition, symposium, etc. 

Number of participants：500

Type：Scientific advice/Review 

Type：Academic society, research group, etc. 

Type：Scientific advice/Review 

Type：Competition, symposium, etc. 

Number of participants：500

Type：Competition, symposium, etc. 

Number of participants：20

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Number of participants：1,500

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Number of participants：15

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Number of participants：20

Type：Academic society, research group, etc. 

Type：Academic society, research group, etc.