担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1152/ajpheart.00241.2016

担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Medical Engineering and Physics  

Despite numerous studies on cerebral arterial blood flow, there has not yet been a comprehensive description of hemodynamics in patients undergoing debranching thoracic endovascular aortic repair (dTEVAR), a promising surgical option for aortic arch aneurysms. A phase delay of the flow rate in the left vertebral artery (LVA) in patients after dTEVAR compared to those before was experimentally observed, while the phase in the right vertebral artery (RVA) remained almost the same before and after surgery. Since this surgical intervention included stent graft implantation and extra-anatomical bypass, it was expected that the intracranial hemodynamic changes due to dTEVAR were coupled with fluid flow and pulse waves in cerebral arteries. To clarify this issue, a one-dimensional model (1D) was used to numerically investigate the relative contribution (i.e., local vessel stiffness and flow path changes) of the VA flow rate to the phase difference. The numerical results demonstrated a phase delay of flow rate in the LVA but not the RVA in postoperative patients undergoing dTEVAR relative to preoperative patients. The results further showed that the primary factor affecting the phase delay of the flow rate in the LVA after surgery compared to that before was the bypass, i.e., alteration of flow path, rather than stent grafting, i.e., the change in local vessel stiffness. The numerical results provide insights into hemodynamics in postoperative patients undergoing dTEVAR, as well as knowledge about therapeutic decisions.

DOI： 10.1016/j.medengphy.2025.104348

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担当区分：筆頭著者,　最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：In Vitro Cellular and Developmental Biology Animal  

Yes-associated protein (YAP), an important downstream mediator of the Hippo pathway, exhibits nuclear translocation when it is dephosphorylated. However, its interplay with other intracellular substances has not yet been clarified. Here, we explored mechano-induced YAP nuclear translocations and its interplay with importin-β (Impβ), one of the nucleocytoplasmic transport receptors, through live imaging of the spatiotemporal distribution of intracellular YAP and Impβ following mechanical stimulation, represented by the release of intercellular tension. YAP nuclear translocation was impeded by inhibition of Impβ and F-actin polymerization. Specifically, inhibiting F-actin polymerization not only impeded the nuclear import of YAP but also prevented its translocation from the near regions, adjacent to the wounded cell, toward the nucleus. These results provide a fundamental basis for further research into mechanotransduction, particularly mechano-induced YAP translocation, and may potentially be exploited as a therapeutic target for wound healing.

DOI： 10.1007/s11626-025-01054-x

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その他リンク： https://link.springer.com/article/10.1007/s11626-025-01054-x/fulltext.html

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Biochemical and Biophysical Research Communications  

Protein kinase Cα (PKCα) has an important role in directed cell migration. After a mechanical wounding, PKCα rapidly accumulates at cell edges adjacent to the wounded cell and regulates cell migration. However, the proteins downstream of PKCα that mediate directed signaling remain unknown. In this study, we examined the spatiotemporal dynamics of PKCα, PIP3, Moesin, Cdc42, MARCKS, Scribble, and Arf6 before directed migration. After wounding, PIP3, Moesin, and Cdc42 accumulated at the cell edge near the wounded cells later than PKCα. In contrast, MARCKS moved away from the plasma membrane without polarization, and Scribble and Arf6 exhibited no significant translocation. The inhibition of PIP3 suppressed the accumulation of Moesin and Cdc42, suggesting that PIP3 regulates Moesin and Cdc42. In particular, the inhibition of PKCα completely inhibited the translocation of all factors, indicating that PKCα is a central regulator in early signaling after wounding and before directional migration.

DOI： 10.1016/j.bbrc.2025.151371

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier BV  

間質流を用いたヒトiPS/ES細胞由来小腸モデルの開発を、京都大学iPS細胞研究所との国内共同研究として達成した。

DOI： 10.1016/j.stem.2024.06.012

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier BV  

DOI： 10.1016/j.rineng.2024.102304

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：AIP Publishing  

We present a numerical analysis of the rheology of a suspension of red blood cells (RBCs) for different volume fractions in a wall-bounded, effectively inertialess, small amplitude oscillatory shear (SAOS) flow for a wide range of applied frequencies. The RBCs are modeled as biconcave capsules, whose membrane is an isotropic and hyperelastic material following the Skalak constitutive law. The frequency-dependent viscoelasticity in the bulk suspension is quantified by the complex viscosity, defined by the amplitude of the particle shear stress and the phase difference between the stress and shear. SAOS flow basically impedes the deformation of individual RBCs as well as the magnitude of fluid-membrane interactions, resulting in a lower specific viscosity and first and second normal stress differences than in steady shear flow. Although it is known that the RBC deformation alone is sufficient to give rise to shear-thinning, our results show that the complex viscosity weakly depends on the frequency-modulated deformations or orientations of individual RBCs but rather depends on combinations of the frequency-dependent amplitude and phase difference. The effect of the viscosity ratio between the cytoplasm and plasma and of the capillary number is also assessed.

DOI： 10.1063/5.0196272

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：特定非営利活動法人 日本バイオレオロジー学会  

Directional migration of eukaryotic cells results from an interplay between cell motility and environmental cues such as stiffness, roughness, and microtopological features of substrates. While the effects of aligned grooves on the substrate lithographically designed on active cell migration are well documented, the impact of unorganized and wrinkled substrates remains poorly understood. We investigated how microscale wrinkles on gelatin substrates regulate two-dimensional (2D) migration of immortalized human mesenchymal stem cells (iMSCs). We showed that there exists an optimal wrinkle size to enhance iMSC migration, where the iMSCs exhibited larger travel distance with moderate wrinkle size (30 μm) than shorter or larger wrinkle sizes (10 μm and 50 μm). These findings reveal a size-dependent effect of wrinkles on iMSC migration and provide insights into designing biomaterials to control stem cell behavior in regenerative medicine applications.

DOI： 10.17106/jbr.38.120

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：特定非営利活動法人 日本バイオレオロジー学会  

The fluidity of endothelial cell (EC) membranes, consisting of a lipid bilayer and heterogeneous multicom-ponent, undergoes alterations in response to shear stress. Although localized variations in membrane fluidity are assumed to induce region-specific signal transduction by modulating membrane protein dynamics on almost planar bilayers, membrane protein diffusion under shear stress remains uncertain. Hence, this study aimed to quantify membrane protein diffusion on ECs under fluid shear stress. We used the photochromic fluorescent protein Dronpa to tag a glycosylphosphatidylinositol-anchored protein (GPI-AP), which diffuses across the outer membranes, and quantified its surface diffusion based on the spatiotemporal distribution of Dronpa-Green-labeled GPI-AP (DGGPI-AP) on EC membranes. We developed an experimental platform to meas-ure the GPI-AP surface diffusion under fluid shear stress and quantified the diffusion coefficient of GPI-AP in two distinct membrane regions: upstream and downstream relative to the direction of fluid flow. Our experimental results showed that there were not statistically significant differ-ences in GPI-AP diffusion on EC membranes between the time points or between the upstream and downstream regions of ECs for at least 15 minutes under shear stress. Our developed methodology and experimental results will be useful to understand a relationship between the membrane protein diffusion and shear-induced cellular processes.

DOI： 10.17106/jbr.38.88

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：American Physical Society (APS)  

DOI： 10.1103/physrevfluids.8.l061101

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その他リンク： http://harvest.aps.org/v2/journals/articles/10.1103/PhysRevFluids.8.L061101/fulltext

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Springer Science and Business Media LLC  

DOI： 10.1007/s00383-022-05228-6

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その他リンク： https://link.springer.com/article/10.1007/s00383-022-05228-6/fulltext.html

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Springer Science and Business Media LLC  

DOI： 10.1007/s11517-022-02649-2

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その他リンク： https://link.springer.com/article/10.1007/s11517-022-02649-2/fulltext.html

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Springer Science and Business Media LLC  

DOI： 10.1007/s11517-022-02601-4

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その他リンク： https://link.springer.com/article/10.1007/s11517-022-02601-4/fulltext.html

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Japan Society of Mechanical Engineers  

DOI： 10.1299/jbse.22-00014

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1098/rsif.2021.0554

担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.3390/mi12101162

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1016/j.jtbi.2021.110709

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1007/s10439-020-02706-7

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1007/s11517-018-1928-7

担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1299/jbse.18-00535

担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.3390/mi10030199

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1038/s41598-017-05924-7

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1103/PhysRevE.92.063011

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.14814/phy2.12037

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1299/kikaib.77.2181

開催年月日： 2025年5月

記述言語：日本語   会議種別：口頭発表（招待・特別）  

開催地：神奈川県横浜市   国名：日本国  

開催年月日： 2024年11月

記述言語：日本語   会議種別：口頭発表（招待・特別）  

開催地：名古屋工業大学   国名：日本国  

開催年月日： 2024年11月

記述言語：英語   会議種別：口頭発表（招待・特別）  

開催地：Kitakyushu International Conference Center   国名：日本国  

開催年月日： 2023年7月

記述言語：英語   会議種別：口頭発表（招待・特別）  

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記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）   出版者・発行元：MDPI AG  

Microfluidics is a powerful tool to precisely control fluids as well as to manipulate suspended small particles in a micrometer-sized space [...]

DOI： 10.3390/mi13071127

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