WADA KENICHI
Organization
Institute for Materials Chemistry and Engineering Department of Applied Molecular Chemistry Academic Researcher
Title
Academic Researcher
Contact information
Tel
0926426337
External link
ORCID
Degree
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Ph.D.
Research History
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独立行政法人 物質・材料研究機構 独立行政法人 理化学研究所 発生・再生科学総合研究センター （神戸） 独立行政法人 理化学研究所 （和光）
Research Interests・Research Keywords
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Research theme： Development of cell engineering technology using microdevice
Keyword： Microfluidic device, Cytoplasmic transfer, Organelle, Mitochondrial genome
Research period： 2019.9 - 2022.6
Awards
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優秀研究賞
2021.5 化学とマイクロ･ナノシステム学会 マイクロ流体デバイスを用いたミトコンドリアゲノム置換
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優秀ポスター賞
2016.12 日本分子生物学会 直接的な細胞質移植を実現するマイクロ流体デバイスを用いた新規単一細胞操作技術
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高木賞
2007.3 インテリジェント材料・システム研究会 生きた細胞と微小流路を用いた毒性センサーチップ
Papers
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Generation of transmitochodrial cybrids using a microfluidic device Reviewed International journal
Ken-Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, Mizuo Maeda, Yui Harada, Yoshikazu Yonemitsu
Experimental Cell Research (2022, in press) 2022.6
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Quantitative control of mitochondria transfer between live single cells using a microfluidic device Reviewed International journal
Ken-Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, Mizuo Maeda
"Biology Open 6: 1960-1965, 2017 doi: 10.1242/bio.024869" 2022.6
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Cell fusion through a microslit between adhered cells and observation of their nuclear behavior Reviewed International journal
Ken-Ichi Wada, K. Hosokawa, E. Kondo, Y. Ito, M. Maeda
Biotechnology and Bioengineering 111: 1464-1468, 2014 2014.6
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Hippo pathway regulation by cell morphology and stress fibers Reviewed International journal
Ken-Ichi Wada, Kazuyoshi Itoga, Teruo Okano, Shigenobu Yonemura, Hiroshi Sasaki
Development 138: 3907-3914, 2011 2011.4
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Live cells-based cytotoxic sensorchip fabricated in a microfluidic system Reviewed International journal
Ken-Ichi Wada, Akiyoshi Taniguchi, Jun Kobayashi, Masayuki Yamato, Teruo Okano
Biotechnology and Bioengineering 99: 1513-1517, 2008 2008.4
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No decrease in myonuclear number after long-term denervation in mature mice Reviewed International journal
Ken-Ichi Wada, Hideyuki Takahashi, Shigeru Katsuta, Hideaki Soya
American Journal of Physiology Cell Physiology 283: C484-488, 2002 2002.4
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Microfluidic perfusion cell culture system confined in 35 mm culture dish for standard biological laboratories Reviewed International journal
Eitaro Kondo, Ken-Ichi Wada, Kazuo Hosokawa, Mizuo Maeda
Journal of Bioscience and Bioengineering 118: 356-358, 2014 2022.4
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A microfluidic device for modulation of organellar heterogeneity in live single cells Reviewed International journal
Ken-Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, Mizuo Maeda
Analytical Sciences 37: 499-505, 2021 2021.6
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Cytoplasmic fusion between an enlarged embryonic stem cell and a somatic cell using a microtunnel device Reviewed International journal
Seong Min Kim, Ken-Ichi Wada, Masashi Ueki, Kazuo Hosokawa, Mizuo Maeda, Yasuyuki Sakai, Yoshihiro Ito
Biochemical and Biophysical Research Communications 520: 527-262, 2019 2019.6
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Cryopreservation of adhered mammalian cells on a microfluidic device: toward ready-to-use cell assay platforms Reviewed International journal
Eitaro Kondo, Ken-Ichi Wada, Kazuo Hosokawa, Mizuo Maeda
Biotechnology and Bioengineering 113: 237-240, 2016 2016.6
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The significance of membrane fluidity of feeder cell-derived substrates for maintenance of iPS cell stemness Reviewed International journal
Yue Zhou, Hongli Mao, Binata Joddar, Nobuhisa Umeki, Yasushi Sako, Ken-Ichi Wada, Chieko Nishioka, Eiki Takahashi, Yi Wang, Yoshihiro Ito
Scientific Reports 5: 11386, 2015 2015.6
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Effects of ROCK inhibitor Y-27632 on cell fusion through a microslit Reviewed International journal
Ken-Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, Mizuo Maeda
Biotechnology and Bioengineering 112: 2334-2342, 2015 2015.6
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Modulating F-actin organization induces organ growth by affecting the Hippo pathway Reviewed International journal
Leticia Sansores-Garcia, Wouter Bossuyt, Ken-Ichi Wada, Shigenobu Yonemura, Chunyao Tao, Hiroshi Sasaki, Georg Halder
EMBO Journal 30: 2325-2335, 2011 2011.4
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Reproducible fashion of the HSP70B' promoter-induced cytotoxic response on a live-cell-based biosensor by cell cycle synchronization Reviewed International journal
Satoshi Migita, Ken-Ichi Wada, Akiyoshi Taniguchi
Biotechnology and Bioengineering 107: 561-565, 2010 2010.4
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DNA damage sensible engineered promoter for cellular biosensing of cytotoxicity Reviewed International journal
Ken-Ichi Wada, Yu Hamaguchi, Kiyoshi Furukawa, Akiyoshi Taniguchi
Biotechnology and Bioengineering 102: 1460-1465, 2009 2009.4
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Formation process and fate of the nuclear chain after injury in regenerated myofiber Reviewed International journal
Ken-Ichi Wada, Shigeru Katsuta, Hideaki Soya
The Anatomical Record 291: 122-128, 2007 2007.4
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Highly sensitive detection of cytotoxicity using a modified HSP70B' promoter Reviewed International journal
Ken-Ichi Wada, Akiyoshi Taniguchi, Teruo Okano
Biotechnology and Bioengineering 97: 871-876, 2007 2007.4
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Rapid and highly sensitive detection of cadmium chloride induced cytotoxicity using the HSP70B' promoter in live cells Reviewed International journal
Ken-Ichi Wada, Akiyoshi Taniguchi, Liming Xu, Teruo Okano
Biotechnology and Bioengineering 92: 410-415, 2005 2005.4
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Natural occurrence of myofiber cytoplasmic enlargement accompanied by decrease in myonuclear number Reviewed International journal
Ken-Ichi Wada, Shigeru Katsuta, Hideaki Soya
Japanese Journal of Physiology 53: 145-150, 2003 53 ( 2 ) 145 - 150 2003.4
Books
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Quantitatively controlled intercellular mitochondrial transfer by cell fusion-based method using a microfluidic device
Ken-Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, Mizuo Maeda（Role：Joint author）
Humana Press 2021.6
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A novel cell fusion method for direct cytoplasmic transfer using a microfluidic device
Ken-Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, Mizuo Maeda（Role：Joint author）
American Chemical Society 2017.6
Presentations
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マイクロ流体デバイスを用いたオルガネラ組成操作
和田健一、細川和生、伊藤嘉浩、前田瑞夫
第44回日本分子生物学会年会 2021.12
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GENERATION OF TRANSMITOCHONDRIAL CYBRID USING A MICROFLUIDIC DEVICE
Ken-Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, Mizuo Maeda, Yui Harada, Yoshikazu Yonemitsu
The 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021) 2021.10
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マイクロ流体デバイスを用いたミトコンドリアゲノム置換
和田健一、細川和生、伊藤嘉浩、前田瑞夫
化学とマイクロ･ナノシステム学会 第43回研究会（CHEMINAS43） 2021.5
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マイクロデバイスを用いた細胞融合技術とオルガネラ研究への応用 Invited International conference
和田健一
第15回ナノ・バイオメディカル学会 2020.11
MISC
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マイクロ流体デバイスを用いたミトコンドリアゲノム置換
和田健一．細川和生．伊藤嘉浩，前田瑞夫
化学とマイクロ・ナノシステム 2021.6
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マイクロデバイスを用いた細胞操作
和田健一
バイオマテリアル‐生体材料‐ 2014.6
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ナノバイオ技術を用いた新規培養システムの開発
谷口彰良，和田健一，大野まき
薬学雑誌 2010.6
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神経や筋を創る細胞
和田健一
体育の科学 2002.6
Industrial property rights
| Patent | Number of applications: 3 | Number of registrations: 0 |
| Utility model | Number of applications: 0 | Number of registrations: 0 |
| Design | Number of applications: 0 | Number of registrations: 0 |
| Trademark | Number of applications: 0 | Number of registrations: 0 |
Professional Memberships
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The Molecular Biology Society of Japan
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The Japanese Biochemical Society
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The Japanese Society for Biomaterials
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Japanese Society of Developmental Biologists
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Japan Society for Cell Biology
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Society for Chemistry and Micro-Nano Systems
Academic Activities
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プログラム委員 International contribution
第28回日本遺伝子細胞治療学会 （ Japan ） 2022.7
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Screening of academic papers
Role(s)： Peer review
2022
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Screening of academic papers
Role(s)： Peer review
2021
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Screening of academic papers
Role(s)： Peer review
2019
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Screening of academic papers
Role(s)： Peer review
2018
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Screening of academic papers
Role(s)： Peer review
2017
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Screening of academic papers
Role(s)： Peer review
2012
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Screening of academic papers
Role(s)： Peer review
2007
Research Projects
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独自に発見した全く新しい phenotype の NK 様細胞を用いた 養子免疫技術の実用化開発
2021.7 - 2024.3
九州大学
Authorship：Coinvestigator(s)
GAIA-102の「腹腔内投与の安全性」ならびに「有効性に関わる臨床POC」を得るために、「腹膜播種を併発する進行・再発胃癌に対する臨床第I/II相試験（医師主導治験）」のうち、第I相部分を完遂することを目的とする。
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合成DNAによるミトコンドリアゲノムの全置換
2021.4 - 2025.3
九州大学
Authorship：Principal investigator
申請者独自のホモプラズミックなmtDNA改変技術を用いて外来mtDNA受容細胞を創出することで合成DNAによるmtDNAの全置換を実現し、mtDNA研究を飛躍的に加速させる研究プラットフォームを創出することを目的とする。
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我々が新しく発見したNK細胞subsetの機能解明と養子免疫治療への応用
2021.4 - 2023.3
九州大学
Authorship：Coinvestigator(s)
Emergency NK’(GAIA-102)の生 物学的な意義とその特性を、特にmemory-like NKとの異同の観点から解析し、臨床応用の ための基盤データを取得することを目的とする。
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合成DNAによるミトコンドリアゲノムの全置換
Grant number：21H02467 2021 - 2025
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Authorship：Principal investigator Grant type：Scientific research funding
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ホモプラズミックなミトコンドリアゲノム改変細胞ライブラリーの創出
Grant number：16K07207 2016 - 2018
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Authorship：Principal investigator Grant type：Scientific research funding
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マイクロデバイスで実現するホモプラズミックなミトコンドリアゲノム改変技術
Grant number：26650068 2014 - 2015
Grants-in-Aid for Scientific Research Grant-in-Aid for challenging Exploratory Research
Authorship：Principal investigator Grant type：Scientific research funding
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形が制御するYapを介した細胞増殖制御の分子機構の解明
Grant number：23770236 2011 - 2012
Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
Authorship：Principal investigator Grant type：Scientific research funding