Language：English   Publishing type：Research paper (scientific journal)  

In seed treatment using atmospheric-pressure air plasmas, deconvoluting plasma-generated reactive species and elucidating seed response mechanisms for each species are crucial. We examined the roles of atomic oxygen and ozone in rice seed germination and found that irradiation with atomic oxygen (0.74 ppm) enhanced germination, whereas exposure to ozone (400 ppm) showed no effect. Protein carbonylation was altered under atomic oxygen irradiation but remained unchanged with ozone exposure. These results indicate that even at low concentrations, atomic oxygen exhibits high reactivity that triggers seed responses, highlighting its key role in germination enhancement.

DOI： 10.35848/1882-0786/ae420c

Open data URL： https://iopscience.iop.org/article/10.35848/1882-0786/ae420c

Language：English  

Cold atmospheric plasma (CAP) is gaining attention as an eco-friendly and sustainable technology in modern agriculture. This study explores the effects of corona discharge CAP on wheat (Triticum aestivum L.) seed performance and yield under field conditions. Seeds were exposed to plasma for 2 s before sowing. Field trials in Tashkent, Fergana, and Syrdarya assessed plant development, pigment levels, and yield components. CAP-treated seeds showed significant increases in chlorophyll a, chlorophyll b, and carotenoids. In Tashkent, plant density improved by 12.9%, grain number per spike by 39%, and yield by 24.4%, with similar effects in other regions. These results suggest CAP enhances wheat growth, photosynthesis, and productivity, offering a chemical-free, cost-effective strategy for improving crop performance.

DOI： 10.1002/ppap.70093

Open data URL： https://onlinelibrary.wiley.com/doi/full/10.1002/ppap.70093

Language：English   Publishing type：Research paper (scientific journal)  

High durability of hydrogenated amorphous carbon (a-C:H) films is one of the significant interests for improving their performance as hard masks in semiconductor processes. In this study, to achieve high-density, low-stress a-C:H films with high deposition rates, we used plasma chemical vapor deposition (CVD) to fabricate a-C:H films using uncommon hydrocarbons precursors like cumene (C9H12) and compared the film deposition characteristics with conventional hydrocarbons precursors like methane (CH4) and acetylene (C2H2). We evaluated the deposition characteristics of a-C:H films using all the precursors in terms of their concentration, reactor pressure, and self-bias voltage. Additionally, we analyzed the structure of the a-C:H films using Raman spectroscopy. Among all the films deposited, the deposition characteristics (mass density, deposition rate, and stress) of films deposited using cumene demonstrated superior characteristics compared to other molecules, suggesting its potential suitability for advanced applications. The films for cumene showed a wide variation of mass density from 1.0 g/cm3 to 2.0 g/cm3 with changing self-bias voltage. At low self-bias voltages (60 V–230 V), the films were polymer-like with a high hydrogen content, whereas, at high self-bias voltages (580 V–1100 V), the films were hard with a low hydrogen content. On the other hand, each film with methane and acetylene showed a narrower variation of mass density from 1.4 g/cm3 to 2.0 g/cm3 with changing self-bias voltage than that for cumene. These results suggest that the film structure can be diversely controlled through the self-bias voltage depending on the number of carbon atoms in one molecule rather than the type of molecular species.

Open data URL： https://www.sciencedirect.com/science/article/pii/S0925963525005254

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Environmental Research  

DOI： https://doi.org/10.1016/j.envres.2024.118760

Open data URL： https://www.sciencedirect.com/science/article/pii/S0013935124006649#:~:text=Therefore,%20developing%20efficient,

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1002/ppap.202200137

Other Link： https://onlinelibrary.wiley.com/doi/10.1002/ppap.202200137

Language：Others   Publishing type：Research paper (scientific journal)  

Cold atmospheric plasma (CAP) is a promising technology for several medical applications, including the removal of biofilms from surfaces. However, the molecular mechanisms of CAP treatment are still poorly understood. Here we unravel the possible mechanisms of CAP-induced oxidation of oligosaccharides, employing reactive molecular dynamics simulations based on the density functional-tight binding potential. Specifically, we find that the interaction of oxygen atoms (used as CAP-generated reactive species) with cellotriose (a model system for the oligosaccharides) can break structurally important glycosidic bonds, which subsequently leads to the disruption of the oligosaccharide molecule. The overall results help to shed light on our experimental evidence for cellotriose oxidation by CAP. This study provides atomic-level insight into the onset of plasma-induced removal of biofilms, as oligosaccharides are one of the main components of biofilm.

DOI： 10.1002/ppap.202200137

Language：English   Publishing type：Research paper (scientific journal)  

Other Link： https://www.mdpi.com/1422-0067/23/11/6330

Language：Others   Publishing type：Research paper (scientific journal)  

The advancement of the information age has intensified the focus on photosensitive materials for information storage devices. To develop new photosensitive two azobenzene side-chain polymers i.e., poly(E)-3-(4-((4-nitrophenyl)diazenyl)phenoxy)propyl acrylate (polymer-1) and poly(E)-3-(4-((2-methoxy-4-nitrophenyl)diazenyl)phenoxy)propyl acrylate (polymer-2), were developed, and their diffraction efficiency was evaluated. The impact of ionic liquids (ILs) on the diffraction efficiency was evaluated by combining the polymers with imidazolium and ammonium families of ILs such as 1-butyl-3-methylimidazolium bromide [Bmim]Br, 1-ethyl-3-methyl-imidazolium-bromide [Emim]Br (imidazolium ILs), and triethylammonium methanesulfonate [TMEAS] (ammonium IL). The molecular interaction of both azobenzene side-chain polymers with the ILs was evaluated before the diffraction efficiency studies by employing UV–vis, FT-IR, and confocal Raman spectroscopies. The spectroscopic studies revealed the interaction of the polymers with the imidazolium and ammonium ILs. The mean diffraction efficiency of polymers-1 and −2 were ∼0.05 and ∼0.022%, respectively. After the addition of the ILs, the diffraction efficiency increased. The highest diffraction efficiency was achieved with the polymer-2 + [Emim]Br system of 3.5% and polymer-2 + TEMS combination of 4.03%. Therefore, although the diffraction efficiency of polymer-1 was higher than that of polymer-2, after adding the ILs, the diffraction efficiency of polymer-2 surpassed that of the polymer-1 + ILs system.

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.jscs.2022.101485

Other Link： https://www.sciencedirect.com/science/article/pii/S1319610322000679?via%3Dihub

Authorship：Lead author,　Corresponding author   Language：Others   Publishing type：Research paper (scientific journal)  

The use of low-temperature plasma for the pre-sowing seed treatment is still in the early stage of research; thus, numerous factors affecting germination percentage, seedling growth, and yield remains unknown. This study aimed to estimate how two critical factors, such as harvest year and seed coat color, affect the percentage of germination and seedling growth after plasma treatment. Radish seeds stored for 2 and 1 year after harvesting (harvested in 2017 and 2018) were sorted into two colors (brown and grey) to investigate the plasma effect on harvest year and seed coat color. We analyzed the amounts of seed phytohormones and antioxidant (γ-tocopherol) were analyzed using mass spectrometry, and physical changes were studied using SEM, EDX, and EPR to understand the mechanism of plasma-induced changes in radish seeds. The obtained results revealed that plasma treatment on seeds affects the germination kinetics, and the maximal germination percentage depends on seed color and the time of seed storage after harvest. Through this study, for the first time, we demonstrated that physical and chemical changes in radish seeds after plasma treatment depends upon the seed color and harvest year. Positive effects of plasma treatment on growth are stronger for sprouts from seeds harvested in 2017 than in 2018. The plasma treatment effect on the sprouts germinated from grey seeds effect was stronger than sprouts from brown radish seeds. The amounts of gibberellin A3 and abscisic acid in control seeds strongly depended on the seed color, and plasma induced changes were better in grey seeds harvested in 2017. Therefore, this study reveals that Air scalar-DBD plasma's reactive oxygen and nitrogen species (RONS) can efficiently accelerate germination and growth in older seeds.

DOI： 10.1038/s41598-021-81175-x

Language：English  

Dye-sensitized solar cells (DSSCs) are attractive due to their simple fabrication process, low cost, performance stability, and eco-friendly characteristics. The power output (Pout) can be improved by applying an external concentrator, but this is rarely applied because of increasing temperature. Here, we successfully increased the Pout of bifacial DSSCs with the V-shaped plane, and concave mirrors showed an improved power output (Pout: 6.42 and 24.31 mW/cm2, separately) compared to ones without mirrors (4.25 mW/cm2). The high surface temperature on the concave mirror can be reduced with 1 cm mirror distancing followed by a reduction in Pout (19.07 mW/cm2).

DOI： 10.1021/acsaem.1c02774

Language：Others   Publishing type：Research paper (scientific journal)  

The study of protein–protein interactions is of great interest. Several early studies focused on the murine double minute 2 (Mdm2)–tumor suppressor protein p53 interactions. However, the effect of plasma treatment on Mdm2 and p53 is still absent from the literature. This study investigated the structural changes in Mdm2, p53, and the Mdm2–p53 complex before and after possible plasma oxidation through molecular dynamic (MD) simulations. MD calculation revealed that the oxidized Mdm2 bounded or unbounded showed high flexibility that might increase the availability of tumor suppressor protein p53 in plasma-treated cells. This study provides insight into Mdm2 and p53 for a better understanding of plasma oncology.

DOI： 10.3390/ijms22179585

Language：Others   Publishing type：Research paper (scientific journal)  

Bacterial and mammalian proteins, such as lysozyme, are gaining increasing interest as anticancer drugs. This study aims to modify the lysozyme structure using cold atmospheric plasma to boost its cancer cell killing effect. We investigated the structure at acidic and neutral pH using various experimental techniques (circular dichroism, fluorescence, and mass spectrometry) and molecular dynamics simulations. The controlled structural modification of lysozyme at neutral pH enhances its activity, while the activity was lost at acidic pH at the same treatment conditions. Indeed, a larger number of amino acids were oxidized at acidic pH after plasma treatment, which results in a greater distortion of the lysozyme structure, whereas only limited structural changes were observed in lysozyme after plasma treatment at neutral pH. We found that the plasma-treated lysozyme significantly induced apoptosis to the cancer cells. Our results reveal that plasma-treated lysozyme could have potential as a new cancer cell killing drug.

DOI： 10.1016/j.ijbiomac.2021.05.146

Language：Others   Publishing type：Research paper (scientific journal)  

Over the past few years, polymers shown comprehensive utilization in optical devices, solar cells, sensors, and other such devices. However, the efficiency of these devices remains a problem. We have synthesized new thiophene based, lowband gap polymer, poly(2-heptadecyl-4-vinylthieno[3,4-d] [1,3] selenazole) (PHVTS) and investigated the interactions between the PHVTS and ionic liquids (ILs), in this study. We have used imidazolium- and ammonium-family ILs, and studied the interactions using various spectroscopic techniques such UV–visible, FTIR, and confocal Raman spectroscopies. Additionally, we studied surface morphology of the polymer-IL film. Spectroscopic studies show that both families of ILs can interact with the newly synthesized polymer poly(2-heptadecyl-4-vinylthieno[3,4-d] [1,3] selenazole). However, the imidazolium-family Ionic Liquid-polymer (IL-polymer) mixture films show higher conductivities than ammonium-family IL–polymer mixture films.

DOI： 10.1016/j.jscs.2021.101227

Language：Others   Publishing type：Research paper (scientific journal)  

Non-small cell lung cancer (NSCLC) is the most common type of lung cancer, with 85% of all lung cancer reported as NSCLC. Moreover, there are no effective treatments in advanced NSCLC. This study shows for the first time that oral administration of plasma-treated water (PTW) can cure advanced NSCLC. The cold plasma in water generates a cocktail of reactive species, and oral administration of this cocktail to mice showed no toxicities even at the highest dose of PTW, after a single dose and repeated doses for 28 d in mice. In vivo studies reveal that PTW showed favorable anticancer effects on chemo-resistant lung cancer, similarly to gefitinib treatment as a reference drug in a chemo-resistant NSCLC model. The anticancer activities of PTW seem to be involved in inhibiting proliferation and angiogenesis and enhancing apoptosis in the cancer cells. Interestingly, the PTW contributes to enhanced immune response and improved cachexia in the model.

DOI： 10.1088/1361-6463/abdff2

Language：Others   Publishing type：Research paper (scientific journal)  

Triton X-100, DMSO and TiO2 were blended with PEDOT:PSS to produce a PEDOT:PSS/DMSO/TX100/TiO2 CE. A bifacial DSSC based on the PEDOT:PSS/DMSO/TX100/TiO2 CE was fabricated, and its photovoltaic characteristics were obtained under simulated solar light of 100 mWcm−2. Under bifacial illumination, the DSSC with the Pt CE achieved a current density (Jsc) of 16.16 mAcm−2 and system efficiency (Esys) of 8.67% while the DSSC with the PEDOT:PSS/DMSO/TX100/TiO2 CE achieved a Jsc of 17.72 mAcm−2 and a Esys of 9.14%. PEDOT:PSS/DMSO/TX100/TiO2 CE is very stable, has a high transmittance, exhibits high electro-catalytic activity, and is an excellent substitute for the Pt CE.

DOI： 10.1016/j.cplett.2021.138369

Language：Others  

DOI： 10.1021/acsagscitech.0c00070

Language：Others   Publishing type：Research paper (scientific journal)  

The recent outbreak of a novel coronavirus (SARS-CoV-2) has caused substantial public health issues worldwide. Cold atmospheric plasma (CAP) has shown its potential application in sterilization. It would be interesting to check the possible effect of CAP on the structure of the C-terminal domain of SARS-CoV-2 (SARS-CoV-2-CTD) spike protein and the interaction SARS-CoV-2-CTD with human angiotensin-converting enzyme 2 (hACE2). Therefore, we performed molecular dynamics simulations to calculate the root-mean-square deviation, root-mean-square fluctuation, principal component analysis and solvent-accessible surface area of SARS-CoV-2-CTD and the SARS-CoV-2-CTD/hACE2 complex with and without possible oxidation.

DOI： 10.35848/1882-0786/abd717

Language：Others   Publishing type：Research paper (scientific journal)  

As the application of nanotechnology increases continuously, the need for controlled size nanoparticles also increases. Therefore, in this work, we discussed the growth mechanism of carbon nanoparticles generated in Ar+CH4 multi-hollow discharge plasmas. Using the plasmas, we succeeded in continuous generation of hydrogenated amorphous carbon nanoparticles with controlled size (25–220 nm) by the gas flow. Among the nanoparticle growth processes in plasmas, we confirmed the deposition of carbon-related radicals was the dominant process for the method. The size of nanoparticles was proportional to the gas residence time in holes of the discharge electrode. The radical deposition developed the nucleated nanoparticles during their transport in discharges, and the time of flight in discharges controlled the size of nanoparticles.

DOI： 10.3390/pr9010002

Language：English   Publishing type：Research paper (scientific journal)  

NADPH oxidases 1 (NOX1) derived reactive oxygen species (ROS) play an important role in the progression of cancer through signaling pathways. Therefore, in this paper, we demonstrate the effect of cold atmospheric plasma (CAP) on the structural changes of Noxa1 SH3 protein, one of the regulatory subunits of NOX1. For this purpose, firstly we purified the Noxa1 SH3 protein and analyzed the structure using X-ray crystallography, and subsequently, we treated the protein with two types of CAP reactors such as pulsed dielectric barrier discharge (DBD) and Soft Jet for different time intervals. The structural deformation of Noxa1 SH3 protein was analyzed by various experimental methods (circular dichroism, fluorescence, and NMR spectroscopy) and by MD simulations. Additionally, we demonstrate the effect of CAP (DBD and Soft Jet) on the viability and expression of NOX1 in A375 cancer cells. Our results are useful to understand the structural modification/oxidation occur in protein due to reactive oxygen and nitrogen (RONS) species generated by CAP.

DOI： 10.1016/j.ijbiomac.2020.09.120

Language：English   Publishing type：Research paper (scientific journal)  

In recent years, non-thermal plasma (NTP) application in agriculture is rapidly increasing. Many published articles and reviews in the literature are focus on the post-harvest use of plasma in agriculture. However, the pre-harvest application of plasma still in its early stage. Therefore, in this review, we covered the effect of NTP and plasma-treated water (PTW) on seed germination and growth enhancement. Further, we will discuss the change in biochemical analysis, e.g., the variation in phytohormones, phytochemicals, and antioxidant levels of seeds after treatment with NTP and PTW. Lastly, we will address the possibility of using plasma in the actual agriculture field and prospects of this technology.

DOI： 10.3390/PR8081002

Language：English   Publishing type：Research paper (scientific journal)  

Ionic liquids (ILs) are known to provide stability to biomolecules. ILs are also widely used in the fields of chemical engineering, biological engineering, chemistry, and biochemistry because they facilitate enzyme catalyzed reactions and enhance their conversion rate. In this work, we have evaluated the influence of alkyl chain substitution of ammonium ILs such as diethylammonium dihydrogen phosphate (DEAP) and triethylammonium hydrogen phosphate (TEAP) for the stability and activity of the tobacco etch virus (TEV) protease. Further, we performed molecular dynamics (MD) simulations to calculate the RMSD (root mean square deviation) for TEV and TEV + ILs. Experimental and simulations results show that TEV is more stable in the presence of TEAP than DEAP. Whereas, TEV protease activity for the cleavage of fusion proteins is preserved in the presence of DEAP while lost in the presence of TEAP. Hence, DEAP IL can serve as alternative solvents for the stability of the TEV protease with preserved activity. To the best of our knowledge, this is first study to show that ILs can stabilize and maintain the TEV protease cleavage activity.

DOI： 10.1016/j.ijbiomac.2020.03.175

Language：Others  

DOI： 10.1515/9783110650600-003

Language：English   Publishing type：Research paper (scientific journal)  

Recently, cold atmospheric plasma (CAP) treatment on seed germination has emerged as a useful technique to increase agriculture production, although, the mechanism of the cold plasma in seed germination is still under investigation. In this work, we studied the role of pigments in the seed coat of radish sprouts on the electron spin resonance (ESR) signals before and after CAP treatment. Radish sprouts seeds having gray color show enhanced ESR signals after the CAP treatment, whereas, no increased ESR signals were observed for brown color seeds of radish sprouts as compared to their respective control seeds. These results reveal that seeds from the same harvest year having different seed coat colors show different responses to the plasma treatment. Although ESR signal intensity can vary with the harvest year, the change in ESR signal intensity after plasma treatment depends on the seed coat color. Independently on the harvest year (2017 and 2018), CAP increased ESR signals stronger in the grey seeds in comparison to the brown ones. The results indicated that seed coat color may be an important variable for understanding differences in the extent of CAP effects on seeds.

DOI： 10.35848/1347-4065/ab7698

Language：English   Publishing type：Research paper (scientific journal)  

Understanding the folding and stability of membrane proteins is of great importance in protein science. Recently, osmolytes and ionic liquids (ILs) are increasingly being used as drug delivery systems in the biopharmaceutical industry. However, the stability of membrane proteins in the presence of osmolytes and ILs is not yet fully understood. Besides, the effect of oxidative stress on membrane proteins with osmolytes or ILs has not been investigated. Therefore, we studied the influence of osmolytes and ILs as co-solvents on the stability of a model membrane protein (i.e., Bacteriorhodopsin in purple membrane of Halobacterium salinarum), using UV–Vis spectroscopy and molecular dynamics (MD) simulations. The MD simulations allowed us to determine the flexibility and solvent accessible surface area (SASA) of Bacteriorhodopsin protein in the presence and/or absence of co-solvents, as well as to carry out principal component analysis (PCA) to identify the most important movements in this protein. In addition, by means of UV–Vis spectroscopy we studied the effect of oxidative stress generated by cold atmospheric plasma on the stability of Bacteriorhodopsin in the presence and/or absence of co-solvents. This study is important for a better understanding of the stability of proteins in the presence of oxidative stress.

DOI： 10.1016/j.ijbiomac.2020.01.179

Language：English   Publishing type：Research paper (scientific journal)  

Nanomaterials such as carbon nanotubes (CNTs) have been used as an excellent material for catalysis, separation, adsorption and disinfection processes. CNTs have grabbed the attention of the scientific community and they have the potential to adsorb most of the organic compounds from water. Unlike, reverse osmosis (RO), nanofiltration (NF) and ultrafiltration (UF) membranes aligned CNT membranes can act as high-flow desalination membranes. CNTs provide a relatively safer electrode solution for biosensors. The article is of the utmost importance for the scientists and technologists working in water purification technologies to eliminate the water crisis in the future. This review summarizes about the application of CNTs in water purification.

DOI： 10.3390/jcs4030135

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.2174/187152061904190521102345

Other Link： http://www.eurekaselect.com/node/172215/viewhtml/U2s5fVmViVazoVBQqlRGbd01j2UXhRrVok9jtTDBfGdk0RRVjTIxmRExqqekyU1O9Ex6eUW5r2YUChwUBnRifYkMs5Qn7VRMfEZvlTlFw5ZzvB4To2lT6MDBjjTF3RRaqnpOsaXcb1b2lRjRFzFzlbmZtIeGngwWjlh0yaDB3MejEJoMqGRicV3hbCOGiZEQgkE0tTnl1UUXxl

Language：English   Publishing type：Research paper (scientific journal)  

Other Link： https://aip.scitation.org/doi/10.1063/1.5086522

Language：English   Publishing type：Research paper (scientific journal)  

Other Link： http://www.eurekaselect.com/node/167260/article/cold-atmospheric-plasma-activated-solution-a-new-approach-for-cancer-treatment

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1038/s41598-018-29549-6

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1038/s41598-018-28600-w

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1088/1361-6463/aac571

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1155/2018/8275985

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.2174/1871520618666180406121734

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.2298/JSC170527107K

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1039/c7ra13389h

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1038/s41598-018-21001-z

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.2174/187152061806181112124717

Language：English  

The progress of nanotechnology has opened the path for the development of new biomaterials. Recent developments have demonstrated that sol-gel bioceramics play an important role in the biomedical field due to their superior biological and mechanical properties. A bioactive hydroxyapatite (HA) is a common bioceramic that attaches to living tissues in vivo at body temperature. Sol-gel processes are now being used to produce bioactive coatings, powders, and substrates that can facilitate control over biological behavior of proteins and cells with broad clinical applications. It has been found that HA particles can down-regulate the expression of some cancerogenic genes in 432tumor cells. The nano-HA particles from the biodegradation of HA ceramics could penetrate the membranes of tumor cells. A rapid and economic sol-gel method has been designed to synthesize sodium silicate (Na ₂ SiO ₃ ) into silica gel in large quantities. Sol-gel-based bioceramics have the potential to present unexpected excellent biological functions. Thus, the understanding and control of the interactions between bioceramics and biological entities may play one of the leading roles in the development of nanomedicine.

DOI： 10.1201/9781315163598

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1039/c7cp04083k

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1038/s41598-017-08643-1

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1039/c7nj00151g

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1038/s41598-017-02690-4

Language：English  

Responsible for pages：137-150   Language：English  

One of the major sources, which wastes the environment is the wastewater produced from the human activity due to enhanced discharge of toxic and persistent organic pollutants into the fresh water sources like rivers etc. Ground and surface waters contain, generally, impurities including organic pollutants such as pesticides, polychlorinated biphenyls (PCBs), halogenated organic solvents and polycyclic aromatic hydrocarbons (PAHs), discharged from industrial and wastewater treatment plants affect their appearance and may have adverse effects for consumers. Presence of large colloidal particle leads to clogging of filtering devices which increases cost of water purification. Bentonite is a kind of low-cost and easily obtained clay mineral. Dominant clay minerals in bentonites are smectites such as montmorillonite, beidellite, saponite, nontronite and hectronite. Natural Bentonite is effective adsorbent for cations but it shows lower affinity towards negative groups, like phosphate, due to the absence of reactive adsorption sites for anions in water. Bentonite clay can treat oil, sulfate, phosphate and is extremely effective at removing certain cationic components from wastewater and has a remarkable affinity for metals, particularly heavy metals in solution.

Language：English  

Responsible for pages：9781118496305, 63-77   Language：English  

One of the most serious problems facing billions of people today is the availability of fresh water. The conventional water recycling methods like chemical oxidation processes and advanced oxidation processes (AOP) are eff ective but have problems such as high cost, secondary pollution and production of chlorinated species. To overcome these problems nanoparticles are increasingly used. The conventional methods of nanoparticle production usually requires a long time and have a high cost, which render them ineff ective for large-scale production of nanoparticles. Nanoparticle production using the plasma technique overcomes these shortcomings. Additionally, the plasma itself acts as a perfect method for water purification due to the formation of various reactive chemical species such as radicals (OH·, H·, O·, HO ₂ ·) and molecular species (H ₂ O ₂ , H ₂ , O ₂ ). Hence, the use of the combined action of nanoparticles and plasma is a new method for solving water treatment problems.

DOI： 10.1002/9781118939314.ch4

Event date： 2024.12

Language：English   Presentation type：Oral presentation (invited, special)  

Venue：Yokohama   Country：Japan  

Event date： 2024.11

Language：English   Presentation type：Oral presentation (invited, special)  

Venue：Kuala Lumpur   Country：Malaysia  

Event date： 2024.11

Language：English   Presentation type：Oral presentation (invited, special)  

Venue：Malacca   Country：Malaysia  

Event date： 2024.10

Language：English   Presentation type：Oral presentation (invited, special)  

Venue：Yogyakarta   Country：Indonesia  

Event date： 2024.9

Presentation type：Oral presentation (invited, special)  

Country：Japan  

Event date： 2024.9

Language：English   Presentation type：Oral presentation (invited, special)  

Venue：Miyakojima   Country：Japan  

Event date： 2024.7

Language：English   Presentation type：Oral presentation (general)  

Venue：Iwate University, Morioka   Country：Japan  

Event date： 2024.5

Language：English   Presentation type：Symposium, workshop panel (public)  

Country：Thailand  

According to a report by the Energy Information Administration of the U.S. Department of Energy, global energy consumption will rise 28% between 2015 and 2040. (EIA). The most promising method of reducing greenhouse gases in the short to medium term is the conversion of CO2 into sustainable carbonaceous fuels. CO2 is a prospective C1 feedstock that is abundant, affordable, and renewable. It can be used to create high-value compounds like CO. Soil fertility management is vital for farmers. Synthetic Nitrogen (N)-fertilizer has increased by 20-fold in the last 50 years to feed the increasingly hungry population. Nonthermal plasma is a better option, as it can convert CO2 and N2 conversion at near-ambient temperatures. The reduction of CO2 with H2O is carried out using the streamer plasma and the possible production of organic compounds in the liquid phase and the formation of CO in the gas phase. The measured net conversion of CO2 to CO was close to 2 % under optimal conditions. Additionally, we synthesize the N-enriched plant soil (NH4NO3 fertilizer in soil) using the low-temperature and low-pressure plasma [without H2 and external catalyst]. Subsequently, we used plasma N-enriched soil to grow plants (radish and tomato)—the plasma N-enriched soil treatment resulted in significant growth enhancement for both radish and tomato plants. Further, substantial changes in phytohormone and antioxidant levels were observed for the plants grown in plasma N-enriched soil compared to control soil and soil + commercial N-fertilizer. We also performed the 1D and 2D simulations using COMSOL Multiphysics® software to determine how the potential reaction generated the gas phase

Event date： 2024.5

Language：English   Presentation type：Symposium, workshop panel (public)  

Country：Japan  

According to a report by the Energy Information Administration of the U.S. Department of Energy, global energy consumption would rise 28% between 2015 and 2040. (EIA). The EIA analysis predicts that over the same time period, energy-related carbon dioxide (CO2) emissions will rise by 16%, from 33.9 billion metric tonnes in 2015 to 39.3 billion metric tonnes in 2040, to match the rise in energy demand.
The most promising method of reducing greenhouse gases in the short to medium term is the conversion of CO2 into sustainable carbonaceous fuels. CO2 is a prospective C1 feedstock that is abundant, affordable, and renewable that can be used to create high-value compounds like CO.
Nonthermal plasma is a better option, as it can convert CO2 at near-ambient temperature and pressure [1-3]. The reduction of CO2 with H2O carried using the streamer plasma and possible production of organic compounds in liquid phase and formation CO in gas phase. The measured net conversion of CO2 to CO was close to 4 % under optimal conditions. Although we also performed the 2D simulation using COMSOL Multiphysics® software to determine how the potential reaction generated gas phase were diffuse to liquid phase.

Event date： 2024.5

Language：English   Presentation type：Symposium, workshop panel (public)  

Country：Japan  

A proposed technique to convert sporadic sustainable electricity into storable chemical energy is the conversion of CO2 into CO via plasma processing. The two most significant obstacles to developing this technology are proving its viability on an industrial scale and obtaining an effective CO2 conversion with high energy efficiency. In this work, we developed the plasma reactor for effective CO2 conversion. The transformation of CO is monitored by using Fourier Transform Infrared Spectroscopy.

Event date： 2024.5

Language：English   Presentation type：Symposium, workshop panel (public)  

Country：Korea, Republic of  

According to a report by the Energy Information Administration of the U.S. Department of Energy, global energy consumption will rise 28% between 2015 and 2040. (EIA). The most promising method of reducing greenhouse gases in the short to medium term is the conversion of CO2 into sustainable carbonaceous fuels. CO2 is a prospective C1 feedstock that is abundant, affordable, and renewable. It can be used to create high-value compounds like CO. Soil fertility management is of great importance for farmers. The use of synthetic Nitrogen (N)-fertilizer increased by 20 folds in the last 50 years to feed the increasingly hungry population. Nonthermal plasma is a better option, as it can convert CO2 and N2 conversion at near-ambient temperatures [1-3]. The reduction of CO2 with H2O is carried out using the streamer plasma and the possible production of organic compounds in the liquid phase and the formation of CO in the gas phase. The measured net conversion of CO2 to CO was close to 2 % under optimal conditions. Additionally, we synthesize the N-enriched plant soil (NH4NO3 fertilizer in soil) using the low-temperature and low-pressure plasma [without H2 and external catalyst]. Subsequently, we used plasma N-enriched soil to grow plants (radish and tomato)—the plasma N-enriched soil treatment resulted in significant growth enhancement for both radish and tomato plants. Further, substantial changes in phytohormone and antioxidant levels were observed for the plants grown in plasma N-enriched soil compared to control soil and soil + commercial N-fertilizer. We also performed the 1D and 2D simulations using COMSOL Multiphysics® software to determine how the potential reaction generated the gas phase

Event date： 2024.5

Language：English   Presentation type：Symposium, workshop panel (public)  

Country：Japan  

Biochemical studies of tissue reveal that the endogenous levels of antioxidant enzymes vary greatly across tissue types; this reflects differences in development and metabolism across different organ systems. In most cancer cells, the intrinsic levels of antioxidant enzymes are low compared to healthy or non-transformed cells. This suggests that most cancer cells lack the biochemical machinery to metabolize H2O2 effectively. Plasma medicine is a new field that uses cold atmospheric plasma (CAP) for various medical applications, such as sterilization, wound healing, blood coagulation, and cancer treatment. CAP interacts with the oxygen, nitrogen, water, etc., in the air to produce various radical and non-radical species, for example, hydroxyl radicals (•OH), superoxide (O2•−), singlet oxygen (1O2), nitrogen dioxide (NO2), hypochlorite (ClO−), atomic oxygen (O), and nitric oxide (NO). During the plasma–liquid interactions, some relatively long-lifetime reactive species are generated in liquid, such as hydrogen peroxide (H2O2), nitrites (NO2−), and nitrates (NO3−). To understand the CAP mechanism in the complex bio-organism, it is essential to understand CAP action on the proteins.

Event date： 2024.5

Language：English   Presentation type：Symposium, workshop panel (public)  

Country：Japan  

Biochemical studies of tissue reveal that the endogenous levels of antioxidant enzymes vary greatly across tissue types; this reflects differences in development and metabolism across different organ systems. In most cancer cells, the intrinsic levels of antioxidant enzymes are low compared to healthy cells or non-transformed cells. This suggests that most cancer cells lack the biochemical machinery to metabolize H2O2 effectively. Plasma medicine is a new field that uses cold atmospheric plasma (CAP) for various medical applications, such as sterilization, wound healing, blood coagulation, and cancer treatment. CAP interacts with the oxygen, nitrogen, water, etc., in air to produce various radical and non-radical species, for example, hydroxyl radicals (•OH), superoxide (O2•−), singlet oxygen (1O2), nitrogen dioxide (NO2), hypochlorite (ClO−), atomic oxygen (O), and nitric oxide (NO). During the plasma–liquid interactions, some relatively long-lifetime reactive species are generated in liquid, such as hydrogen peroxide (H2O2), nitrites (NO2−), and nitrates (NO3−). To understand the CAP mechanism in the complex bio-organism, it is important to understand the CAP action of proteins. To understand the plasma treatment effect on the structure and activity of catalase, we treated bovine liver catalase using pulsed DBD and Jet for 5, 10, and 20 min. We investigated the structural and thermodynamic changes in catalase through UV-visible, fluorescence, and circular dichroism spectroscopies after DBD and Jet plasma treatments at various time intervals. We have also performed mass spectrometry analysis to reveal the possible modified amino acids in catalase after both types of plasma treatments (DBD and jet). The jet plasma treatment modifies the catalase structure to a greater extent than the DBD plasma. Additionally, we performed molecular dynamics simulations based on the mass spectrometry analysis to gain insight into the structural deformation of catalase. Finally, we have observed that inhibition of catalase enzyme in A375 cancer cells increases the plasma treatment (DBD and jet) efficiency. Molecular dynamic simulation studies show more change in RMSD for catalase after Jet treatment than DBD treatment. Therefore, we conclude that the enzymatic activity of catalase in cancer cells decreases after plasma treatment.

Event date： 2024.5

Language：English   Presentation type：Symposium, workshop panel (public)  

Country：Japan  

Plasma medicine represents an emerging field harnessing the power of cold atmospheric plasma (CAP) across a wide range of medical applications, including sterilization, wound healing, blood coagulation, and cancer treatment. CAP interacts dynamically with elements like oxygen, nitrogen, and water in the air, giving rise to a spectrum of radical and non-radical species, including hydroxyl radicals (•OH), superoxide (O2•−), singlet oxygen (1O2), nitrogen dioxide (NO2), hypochlorite (ClO−), atomic oxygen (O), and nitric oxide (NO). During plasma-liquid interactions, certain relatively long-lived reactive species emerge within the liquid, such as hydrogen peroxide (H2O2), nitrites (NO2−), and nitrates (NO3−). Understanding the impact of CAP on complex bio-organisms necessitates a comprehensive grasp of CAP's influence on proteins. The majority of proteins adopt highly compact folded structures, optimizing the polypeptide chain's free energy in conjunction with the surrounding solvent under physiological conditions. In some instances, cells utilize organic osmolytes alongside inorganic solutes, as excessive concentrations of inorganic salts (Na+ or K+) can disrupt protein function, while organic solutes remain innocuous. Proper protein conformation holds pivotal significance for cell viability under physiological conditions. Organic osmolytes facilitate protein folding by shifting the folding equilibrium, thus offering the potential to alleviate challenging diseases stemming from protein misfolding. Protein misfolding threatens cellular homeostasis through either protein degradation or aggregation, culminating in deleterious genetic disorders.
Hence, co-solvents like naturally occurring osmolytes or synthetically produced ionic liquids can serve to stabilize protein folding and function, directing the folding equilibrium away from protein aggregation and/or degradation. Consequently, our research has delved into the impact of CAP on various proteins, including myoglobin, MTH1880, NOXA-SH3 domain, catalase, and G protein-coupled receptors (GPCRs), to elucidate the protein folding mechanisms. Furthermore, we have explored the influence of osmolytes and ionic liquids on protein folding in plasma treatment through a combination of experimental techniques and molecular dynamics simulations. Our experimental investigations have unveiled that amino acid oxidation within proteins increases proportionally with the duration of plasma treatment and pH of solution. This oxidation level significantly affects protein structure and function. In select cases, osmolytes/ionic liquids have shown the capability to shield against the detrimental effects of CAP treatment, although the extent of protection varies depending on the specific protein. In conclusion, co-solvents afford a degree of protection against CAP's impact on bio-organisms. These discoveries shed new light on the interplay between ROS/RNS and proteins, whether co-solvents are present or absent

Event date： 2024.5

Language：English   Presentation type：Symposium, workshop panel (public)  

Country：Thailand  

The use of cold atmospheric plasma (CAP) treatment of seeds benefits over conventional treatments due to short treatment time and low-temperature operations. During CAP interacts with the oxygen, nitrogen, water, etc., in air, they produce various radical and non-radical species. It is a general fact that reactive species like reactive oxygen and nitrogen species (RONS) can influence plant growth and development. The increased nitrogen nutrients level influences growth hormones, activation of growth-related gene expression, and other physiological processes to understand the plasma effect on seedling growth. Additionally, the presence of RONS can disrupt redox homeostasis and cause mild oxidative stress in plants during vegetative and reproductive stages.
Electron paramagnetic resonance (EPR) spectroscopy is optimal for interpreting any change in paramagnetic defect centers. The use of free radical species as precursors to identify the changes in the biological systems due to physical or chemical stress. The detection of the change in paramagnetic species before and after seed treatment is of great interest. Therefore, we used EPR spectroscopy in this study to detect the changes that occurred in seeds before and after plasma treatment. We observed that scalar DBD treatment on radish sprout seed coat increases organic free radical intensity. The weak peak at g = 4.3 represents the signal for Fe3+, hyperfine lines belonging to the Mn2+ peaks, and an intense sharp rise at g = 2.0 attributed to the semiquinone radical.
Further, we treated seeds that matured under heat stress with CAP and found that subsequent germination was significantly restored; genes involved in ABA biosynthesis (OsNCED2 and OsNCED5) were downregulated, whereas genes involved in ABA catabolism (OsABA8′OH1 and OsABA8′OH3) and α-amylase genes (OsAmy1A, OsAmy1C, OsAmy3B, and OsAmy3E) were upregulated. CAP treatment caused sig0.5nificant hypermethylation of the OsNCED5 promoter and hypomethylation of OsAmy1C and OsAmy3E promoters, which matched their expression patterns. We suggest that CAP treatment can significantly improve the germination of rice seeds affected by heat stress by affecting epigenetic regulation.

Event date： 2024.5

Language：English   Presentation type：Symposium, workshop panel (public)  

Country：India  

Biochemical studies of tissue reveal that the endogenous levels of antioxidant enzymes vary greatly across tissue types; this reflects differences in development and metabolism across different organ systems. In most cancer cells, the intrinsic levels of antioxidant enzymes are low compared to healthy or non-transformed cells. This suggests that most cancer cells lack the biochemical machinery to metabolize H2O2 effectively. Plasma medicine is a new field that uses cold atmospheric plasma (CAP) for various medical applications, such as sterilization, wound healing, blood coagulation, and cancer treatment. CAP interacts with the oxygen, nitrogen, water, etc., in the air to produce various radical and non-radical species, for example, hydroxyl radicals (•OH), superoxide (O2•−), singlet oxygen (1O2), nitrogen dioxide (NO2), hypochlorite (ClO−), atomic oxygen (O), and nitric oxide (NO). During the plasma–liquid interactions, some relatively long-lifetime reactive species are generated in liquid, such as hydrogen peroxide (H2O2), nitrites (NO2−), and nitrates (NO3−). To understand the CAP mechanism in the complex bio-organism, it is essential to understand CAP action on the proteins.
To understand the effect of plasma treatment on the structure and activity of catalase, we treated bovine liver catalase using CAP for 5, 10, and 20 min. We investigated the structural and thermodynamic changes in catalase through UV-visible, fluorescence, and circular dichroism spectroscopies after CAP plasma treatments at various time intervals. We have also performed mass spectrometry analysis to reveal the possible modified amino acids in catalase after both types of plasma treatments. Additionally, we performed molecular dynamics simulations based on mass spectrometry analysis to gain insight into the structural deformation of proteins and enzymes. Molecular dynamic simulation studies show more change in RMSD for enzymes after CAP treatment compared to the control structure. Therefore, we conclude that enzymes' enzymatic activity and structure change after plasma treatment.

Event date： 2024.5

Language：English  

Country：Japan  

We hypothesize that the atmospheric pressure non-thermal plasma (simply plasma) can aid in the conversion of CO2 and N2 to plant nutrients via plasma-treated/activated water (PTW). We treated the radish seeds with CO2-generated PTW and N2-generated PTW to determine the germination rate, germination percentage, and seeding growth. We suggested that CO and H2O2 formed during CO2-generated PTW production trigger early germination and growth enhancement.

Event date： 2024.3

Language：English   Presentation type：Oral presentation (invited, special)  

Country：Thailand  

Event date： 2026.3

Language：English   Presentation type：Oral presentation (general)  

In this study, we examined the use of plasma-ILs for CO2 capture and conversion at different wt%. We demonstrated that ILs can be used for CO2 capture and produce CO at low cost. Additionally, some ILs generate high CO levels but have low CO2 capture efficiency due to plasma-induced degradation of the IL. Results showed that ILs are toxic to fibroblast cells, with toxicity influenced by concentration and type, and that plasma treatment did not significantly alter this toxicity. In contrast, the interaction between IL and Hb protein changes with plasma-treated ILs, which interact more strongly with Hb.

Event date： 2025.11

Language：English   Presentation type：Oral presentation (general)  

We used an IL to capture and store CO2, and we used NTP to convert the captured CO2 into CO. In this investigation, we converted collected CO2 to CO using the 1-Butyl-3-methylimidazolium chloride ([Bmim]Cl) ionic liquid. In the previous study, we have shown that [Bmim]Cl ionic liquid not only captures the CO2 from the pure CO2 cylinder and stores it for more than 24 hours at room temperature and atmospheric pressure, but it can also capture the CO2 from the atmospheric air and store it. The use of plasma (streamer plasma) for the separation of CO2 from ionic liquids may lead to the degradation of ionic liquids. This is the first time we are presenting the potential changes in toxicity and degradation of the ionic liquid following plasma treatment. To study the toxicity, we employed MRC-5 (Normal embryonic lung fibroblast) and NIH3T3 (mouse embryonic fibroblast) cells. To study the degradation, we used High-Pressure Liquid Chromatography (HPLC) to observe the changes in ionic liquids before and after plasma treatment. Our study shows that the toxicity of IL increases after 1-minute plasma treatment, compared to the same treatment on water. Additionally, the degradation was also confirmed by the HPLC experiments. Hence, we concluded that the cell death of fibroblast cells is not due to reactive oxygen and nitrogen species (RONS) generated by the streamer plasma, but rather due to the modification of the ionic liquid structure by plasma.

Event date： 2025.10

Language：English   Presentation type：Oral presentation (general)  

We employed an RF-low-pressure plasma operating at various pressures (60, 100, and 15 Pa) with or without the addition of water vapor. Using optical emission spectroscopy (OES), we observed that high concentrations of CO, CO+, CHO, C2, Hα, O2, and O species formed in plasma at 60 Pa. As the pressure increases, the concentration of these species decreases. Later, we used the Specair 2.0 program to calculate the electron temperature, rotational temperature, and vibrational temperature. Furthermore, we also performed a COMSOL Multiphysics simulation to identify the short radical species generated in the plasma

Event date： 2024.9

Presentation type：Oral presentation (invited, special)  

Event date： 2024.6

Language：English   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2024.3

Presentation type：Oral presentation (general)  

Event date： 2024.2

Language：English   Presentation type：Oral presentation (invited, special)