Updated on 2024/10/04

Information

 

写真a

 
HOSAKA YOSHINAO
 
Organization
Faculty of Agriculture Department of Bioresource Sciences Professor
Environmental Control Center for Experimental Biology (Concurrent)
School of Agriculture Department of Bioresource and Bioenvironment(Concurrent)
Graduate School of Bioresource and Bioenvironmental Sciences Department of Bioresource Sciences(Concurrent)
Title
Professor
External link

Research Areas

  • Life Science / Animal physiological chemistry, physiology and behavioral biology

  • Life Science / Laboratory animal science

  • Life Science / Anatomy

Degree

  • DVM

  • Ph.D.

Research History

  • Kyushu University Laboratry of Functional Anatomy, Research Faculty of Agriculture Professor

    2022.10 - Present

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  • 鳥取大学大学院 共同獣医学研究科 基礎獣医科学講座 教授

    2019.4 - 2022.9

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    Country:Japan

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  • Tottori University Faculty of Agriculture Professor

    2014.4 - 2022.9

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  • 農林水産省 秋田市

    農林水産省 秋田市

  • 鳥取大学(2008年4月-2022年9月) 酪農学園大学(2000年4月-2008年3月)

Research Interests・Research Keywords

  • Research theme:骨芽細胞

    Keyword:骨芽細胞

    Research period: 2024

  • Research theme:脂肪

    Keyword:脂肪

    Research period: 2024

  • Research theme:脂肪細胞

    Keyword:脂肪細胞

    Research period: 2024

  • Research theme:間葉系幹細胞

    Keyword:間葉系幹細胞

    Research period: 2024

  • Research theme:

    Keyword:

    Research period: 2024

  • Research theme:プロテオグリカン

    Keyword:プロテオグリカン

    Research period: 2024

  • Research theme:分化

    Keyword:分化

    Research period: 2024

  • Research theme:破骨細胞

    Keyword:破骨細胞

    Research period: 2024

  • Research theme:

    Keyword:

    Research period: 2024

  • Research theme:筋芽細胞

    Keyword:筋芽細胞

    Research period: 2024

  • Research theme:筋衛星細胞

    Keyword:筋衛星細胞

    Research period: 2024

  • Research theme:結合組織

    Keyword:結合組織

    Research period: 2024

  • Research theme:線維芽細胞

    Keyword:線維芽細胞

    Research period: 2024

  • Research theme:グリコサミノグリカン

    Keyword:グリコサミノグリカン

    Research period: 2024

  • Research theme:コンドロイチン硫酸

    Keyword:コンドロイチン硫酸

    Research period: 2024

  • Research theme:Effects of sex hormones and glycosaminoglycans on locomotive organ morphogenesis and functional expression.

    Keyword:Estrogen, chondroitin sulfate, locomotive organ, regulation of differentiation, morphogenesis, functional expression

    Research period: 2008.10

Papers

  • Expression and localization of the neuropeptide Y-Y4 receptor in the chick spleen: mRNA upregulation by high ambient temperature. International journal

    Haruka Nishimura, Mohamed Z Elhussiny, Yoshimitsu Ouchi, Shogo Haraguchi, Taichi Q Itoh, Elizabeth R Gilbert, Mark A Cline, Shotaro Nishimura, Yoshinao Z Hosaka, Eiki Takahashi, John F Cockrem, Takashi Bungo, Vishwajit S Chowdhury

    Neuropeptides   107   102459 - 102459   2024.10   ISSN:0143-4179 eISSN:1532-2785

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    High ambient temperatures (HT) can increase diencephalic neuropeptide Y (NPY) expression, and central injection of NPY attenuates heat stress responses while inducing an antioxidative state in the chick spleen. However, there is a lack of knowledge about NPY receptor expression, and its regulation by HT, in the chick spleen. In the current study, male chicks were used to measure the expression of NPY receptors in the spleen and other immune organs under acute (30 vs. 40 ± 1°C for 3 h) or chronic (30 vs. 40 ± 1°C for 3 h/day for 3 days) exposure to HT and in response to central injection of NPY (47 pmol, 188 pmol, or 1 nmol). We found that NPY-Y4 receptor mRNA was expressed in the spleen, but not in other immune organs studied. Immunofluorescence staining revealed that NPY-Y4 receptors were localized in the splenic pulp. Furthermore, NPY-Y4 receptor mRNA increased in the chick spleen under both acute and chronic exposure to HT. Central NPY at two dose levels (47 and 188 pmol) and a higher dose (1 nmol) did not increase splenic NPY-Y4 receptor mRNA expression or splenic epinephrine under HT (35 ± 1°C), and significantly increased 3-methoxy-4-hydroxyphenylglycol (MHPG) concentrations under HT (40 ± 1°C). In conclusion, increased expression of NPY-Y4 receptor mRNA in the spleen under HT suggest that Y4 receptor may play physiological roles in response to HT in male chicks.

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  • Sex differences in COL1A1 Expression and Collagen Content in Skeletal Muscle of Mature and Juvenile Shamo Chickens. Reviewed International journal

    Nishimura S, Ohtani M, Kabunda GM, Arai S, Nishimura H, Hosaka YZ.

    J Poult Sci.   61 ( 0 )   n/a - 2024011   2024.4   ISSN:13467395 eISSN:13490486

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Japan Poultry Science Association  

    <p>Collagen content is an important parameter affecting meat consistency. Sex differences in collagen were therefore studied in mature and juvenile Shamo chickens. The pectoral (PT), lateral iliotibial (ITL), medial part of puboischiofemoral (PIF), and lateral part of gastrocnemius (GCL) muscles were weighed, and their <i>COL1A1</i> expression levels and total collagen content were analyzed. Body and muscle weights were significantly higher in males than in females of all ages. Muscle/body weight ratios were also higher in mature males than in females, but this difference was not observed in juveniles. In mature chickens, <i>COL1A1</i> expression was higher in the PIF and GCL muscles; this was not the case in juvenile chicken muscles. Sex differences in collagen content were observed only in the ITLs of mature chickens. A positive correlation between muscle weight and intramuscular collagen content was found for PT and GCL, but not for ITL and PIF, muscles. These results suggest that the sex difference in intramuscular collagen content only occurs in specific muscles and that <i>COL1A1</i> expression is not necessarily related to collagen content in mature chickens. Factors that determine the intramuscular collagen content likely differ by muscle type. </p>

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  • Quantitative, compositional, and immunohistochemical analyses of chondroitin sulfate, dermatan sulfate, and hyaluronan in internal organs of deer (Cervus nippon centralis and C. n. yesoensis) and cattle (Bos taurus). International journal

    Naoko Takeda-Okuda, Su-Jung Yeon, Yoshiaki Matsumi, Yoshinori Matsuura, Yoshinao Z Hosaka, Jun-Ichi Tamura

    International journal of biological macromolecules   261 ( Pt 1 )   129680 - 129680   2024.1   ISSN:0141-8130 eISSN:1879-0003

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    Chondroitin sulfate (CS) + dermatan sulfate (DS) and hyaluronan (HA) concentrations and the sulfation patterns of CS-DS in the cartilaginous tissues and alimentary canals of Honshu Sika deer, Hokkaido Sika deer, and cattle were investigated in the present study. CS + DS concentrations were high in cartilaginous tissues, namely, the trachea and scapular cartilage region (5- 12 g*), and low in the alimentary canal (~0.3 g*). HA concentrations were low in cartilaginous tissues and the alimentary canal (~0.2 g*). All tissues mainly contained A-type [HexAGalNAc(4-sulfate)] and C-type [HexAGalNAc(6-sulfate)] CS + DS. The ratios of A-type/C-type CS + DS were 1.2- 3.1 and 0.9- 16.4 in cartilaginous tissues and the alimentary canal, respectively. CS + DS predominantly comprised β-D-GlcA and α-L-IdoA in cartilaginous tissues and the alimentary canal, respectively. The alimentary canal characteristically contained up to 14 % highly sulfated E-type [HexAGalNAc(4,6-disulfate)] and D-type [HexA(2-sulfate)GalNAc(6-sulfate)] CS + DS. The specific distributions of CS and DS were immunohistochemically confirmed using CS + DS-specific antibodies. Although the omasum of cattle is more likely to have higher concentrations of CS + DS and HA, no significant species differences were observed in the concentrations or sulfation patterns of CS + DS among species for Honshu Sika deer, Hokkaido Sika deer, and cattle. (*per 100 g of defatted dry tissue).

    DOI: 10.1016/j.ijbiomac.2024.129680

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  • Qualitative and quantitative analyses in sulfated glycosaminoglycans, chondroitin sulfate/dermatan sulfate, during 3T3-L1 adipocytes differentiation. Reviewed International journal

    Cahyadi DD, Warita K, Takeda-Okuda N, Tamura JI, Hosaka YZ.

    Anim Sci J.   94 ( 1 )   e13894   2023.12   ISSN:1344-3941 eISSN:1740-0929

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    Chondroitin sulfate/dermatan sulfate (CS/DS) is a member of glycosaminoglycans (GAGs) found in animal tissues. Major CS/DS subclasses, O, A, C, D, and E units, exist based on the sulfation pattern in d-glucuronic acid (GlcA) and N-acetyl-d-galactosamine repeating units. DS is formed when GlcA is epimerized into l-iduronic acid. Our study aimed to analyze the CS/DS profile in 3 T3-L1 cells before and after adipogenic induction. CS/DS contents, molecular weight (Mw), and sulfation pattern were analyzed by using high-performance liquid chromatography. CS/DS synthesis- and sulfotransferase-related genes were analyzed by reverse transcription real-time PCR. CS/DS amount was significantly decreased in the differentiated (DI) group compared to the non-differentiated (ND) group, along with a lower expression of CS biosynthesis-related genes, chondroitin sulfate N-acetylgalactosaminyltransferase 1 and 2, as well as chondroitin polymerizing factor. GAGs in the DI group also showed lower Mw than those of ND. Furthermore, the A unit was the major CS/DS in both groups, with a proportionally higher CS-A in the DI group. This was consistent with the expression of carbohydrate sulfotransferase 12 that encodes chondroitin 4-O-sulfotransferase, for CS-A formation. These qualitative and quantitative changes in CS/DS and CS/DS-synthases before and after adipocyte differentiation reveal valuable insights into adipocyte development.

    DOI: 10.1111/asj.13894

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  • Collagen Content and Collagen Fiber Architecture in the Skin of Shamo Chicken, a Japanese Game Fowl Reviewed International journal

    Nishimura S, Arai S, Hosaka YZ.

    J Poult Sci.   60 ( 2 )   n/a - 2023026   2023.10   ISSN:13467395 eISSN:13490486

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    <p>Collagen content and collagen fiber architecture in the skin of Shamo chickens were compared between sexes and body parts. Cervical, thoracic, dorsal, femoral, and crural skin samples were collected and their collagen content was analyzed. Collagen fiber specimens were prepared for scanning electron microscopy using the cell maceration method with a NaOH solution. Sex differences in collagen content were only observed in the femoral skin of mature chickens, but not in 10-week-old chicks. The difference in collagen content between body parts was obvious; femoral and crural skin had higher collagen content than those of other parts in both sexes. Scanning electron microscopy indicated that the collagen fiber architecture was quite different between the superficial and deep layers in the dermis, with the former consisting of loosely tangled band-like collagen fibers, and the latter composed of thick and dense layers of collagen bundles in a parallel arrangement. The width of collagen fibers in the superficial layer of the dermis differed between sexes in the dorsal, femoral, and crural skin. From these results, it is likely that the difference in collagen content in the femoral skin is not due to sex hormones but other factors, such as mechanical stimulation in daily activity. Additionally, collagen fiber width in the superficial layer is likely related to the difference in collagen content between sexes and between body parts.</p>

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  • Housekeeping gene expression variability in differentiating and non-differentiating 3T3-L1 cells. International journal

    Danang Dwi Cahyadi, Tomoko Warita, Nanami Irie, Kana Mizoguchi, Jiro Tashiro, Yoshinao Z Hosaka, Katsuhiko Warita

    Adipocyte   12 ( 1 )   2235081 - 2235081   2023.7   ISSN:2162-3945 eISSN:2162-397X

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    Normalization is a crucial step in gene expression analysis to avoid misinterpretation. Reverse transcription-quantitative polymerase chain reaction was used to measure the expression of 10 candidate housekeeping genes in non-differentiated (ND) and differentiated (DI) 3T3-L1 cells on days 5 and 10. We used geNorm, NormFinder, BestKeeper, RefFinder, and the ∆Ct method to evaluate expression stability. The findings revealed that (1) the expression levels of the reference genes changed over time, even in non-differentiating cells, and (2) peptidylprolyl isomerase A (Ppia) and TATA box-binding protein (Tbp) were stable reference genes for 10 days in both undifferentiated and differentiated 3T3-L1 cells. Notably, the expression of known reference genes in non-differentiating cells was altered throughout the experiment.

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  • Effects of different coating materials on the morphological characteristics of chicken adenohypophyseal folliculo-stellate cells in vitro Reviewed International journal

    Nishimura S, Yamahira S, Chowdhury VS, Hosaka YZ

    Anim Sci J.   94 ( 1 )   e13814   2023.1   ISSN:1344-3941 eISSN:1740-0929

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    Chicken adenohypophyseal cells were cultured in plates coated with different materials, and their morphologies were examined to confirm the characteristics of chicken folliculo-stellate (FS) cells in vitro. The adenohypophyseal cells were dispersed with a collagenase/trypsin mixture in media and seeded in plates coated in either poly L-lysine (PLL), collagen, or laminin. After 7 days of culture, the cells were fixed and immunocytochemistry was performed. 5-Bromo-2'-deoxyuridine incorporation test indicated that the proliferation activity of the culture cells was different based on the coating materials, and it was higher in the collagen-coated plate than two other coating materials. Fluorescence immunocytochemistry was also performed using mixed antibodies against growth hormone, prolactin, luteinizing hormone β-subunit, basic cytokeratin (bCK), and S100B. The culture cells on the PLL- and laminin-coated surfaces were round or oval in shape, and bCK-immunopositive FS cells were morphologically indistinguishable from endocrine cells. In the collagen-coated plate, many endocrine cells were round or oval in shape, but FS cells displayed a larger and flattened morphology. S100B-immunoreactions were localized in the nuclei of bCK-immunopositive FS cells. These results suggest that culturing the chicken adenohypophyseal cells in the collagen-coated plate enables the distinction of FS cells from endocrine cells.

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  • Determination of thick and thin fibres distribution in Sunda porcupine dorsal skin (Hystrix javanica) using Picrosirius red staining. International journal

    Andhika Yudha Prawira, Savitri Novelina, Wartika Rosa Farida, Huda Shalahudin Darusman, Katsuhiko Warita, Yoshinao Z Hosaka, Srihadi Agungpriyono

    Anatomia, histologia, embryologia   51 ( 5 )   666 - 673   2022.7   ISSN:03402096

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    The complexity of the Sunda porcupine skin has become an important topic due to the unique characteristics of its quill follicles. The structure and chemical composition of the skin has affected many physiological and other conditions. Generally, quills are larger, stronger and stiffer than hair; therefore, the skin structure needs to adapt to support their physiology. The strength of the skin is determined by its collagen composition and arrangement; therefore, this study aims to analyse the composition and distribution of thick and thin fibres based on the specific characteristics of Sunda porcupine skin under polarized light using picrosirius red staining. The skin samples used were from the thoracodorsal and lumbosacral regions of eight Sunda porcupine adults. The histological staining was carried out using the picrosirius red method, while the samples were observed under a polarized light microscope and analysed with software. The results showed that the skin is composed of 36%-65% thick fibres, 20%-35% thin fibres and small amounts of other types with the lumbosacral region having higher compositions of thick and thin fibres than those in the thoracodorsal region. Furthermore, the thoracodorsal and lumbosacral regions have the highest composition of thick fibre in the deeper dermis and quill follicle, respectively. These demonstrated that the complexity of the skin structure of Sunda porcupine due to its quill derivates correlated with its collagen composition and distribution.

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  • Chondroitin sulfate E downregulates N-cadherin and suppresses myotube formation.

    Fumi Satoh, Akihiro Sugiura, Jiro Tashiro, Yoshinao Z Hosaka, Katsuhiko Warita

    The Journal of veterinary medical science   84 ( 4 )   494 - 501   2022.4   ISSN:09167250

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    Myogenesis, the formation of muscle fibers, is affected by certain glycoproteins, including chondroitin sulfate (CS), which are involved in various cellular processes. We aimed to investigate the mechanism underlying CS-E-induced suppression of myotube formation using the myoblast cell line C2C12. Differentiated cells treated with 0.1 mg/ml CS-E for nine days showed multinucleated and rounded myotubes with myosin heavy chain positivity. No difference was found between the CS-E-treated group with rounded myotubes and CS (-) controls with elongated myotubes in the levels of phospho-cofilin, a protein involved in the dynamics of actin cytoskeleton. Interestingly, N-cadherin, which is involved in the gene expression of myoblast fusion factors (myomaker and myomixer), was significantly downregulated at both the mRNA and protein levels following CS-E treatment. These results suggest that N-cadherin downregulation is one of the mechanisms underlying the CS-E-induced suppression of myotube formation.

    DOI: 10.1292/jvms.21-0662

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  • Compositional analysis of chondroitin/dermatan sulfate in rhesus monkeys (Macaca mulatta). International journal

    Danang Dwi Cahyadi, Munehiro Okamoto, Katsuhiko Warita, Naoko Takeda-Okuda, Jun-Ichi Tamura, Yoshinao Z Hosaka

    Bioscience, biotechnology, and biochemistry   86 ( 7 )   811 - 818   2022.4   ISSN:09168451

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    Glycosaminoglycans (GAGs) are found in the various tissues and are involved in many physiological functions. Since the rhesus monkey (Macaca mulatta) is the most widely used nonhuman primate in biomedical research, an understanding of the compositions of GAGs in their tissues is important. The aim of the study was to determine the content and sulfation pattern of disaccharides contained in several tissues of the rhesus monkey. The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain was extracted from several tissues of female and male rhesus monkeys. Compositional analysis was performed after digestion with chondroitinases ABC and ACI to reveal the sulfation pattern of the CS/DS hybrid chain. This study revealed that the major CS/DS disaccharide units present in the tissues were A- and C-types. The E- and iE-types were specifically distributed not only in the tracheal tissue but also in gastrointestinal tissues.

    DOI: 10.1093/bbb/zbac057

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  • Mutation in BEIIb mitigates the negative effect of the mutation in ISA1 on grain filling and amyloplast formation in rice

    Nagamatsu, S; Wada, T; Matsushima, R; Fujita, N; Miura, S; Crofts, N; Hosaka, Y; Yamaguchi, O; Kumamaru, T

    PLANT MOLECULAR BIOLOGY   108 ( 4-5 )   497 - 512   2022.3   ISSN:0167-4412 eISSN:1573-5028

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  • Preliminary study of the gene expression of sulfation and degradation enzymes for chondroitin sulfate in glycerol-treated C2C12 myoblast cells. Reviewed

    Yoshinao Z Hosaka, Sota Washie, Katsuhiko Warita

    The Journal of veterinary medical science   84 ( 3 )   306 - 309   2022.1   ISSN:09167250

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    In this study, we induced chemical damage of C2C12 myoblasts that had differentiated into myotubes with glycerol, and four sulfation enzymes for chondroitin sulfate (CS) [carbohydrate sulfotransferase (Chst)12, Chst15 and Chst3) and uronyl 2-O-sulfotransferase (UST)] and two CS degradation enzymes [hyaluronidase (Hyal)1 and Hyal2] were examined for changes in gene expression. Treatment of myoblasts with 5% glycerol significantly increased the expression levels of the sulfation enzymes Chst12 and Chst15 and the degradation enzymes Hyal1 and Hyal2. However, the expression levels of the other two genes (Chst3 and Ust) showed no change. Differences in the expression levels of these enzymes may help to understand the difference in responsiveness of myoblasts to glycerol after muscle injury in vivo or in vitro.

    DOI: 10.1292/jvms.21-0632

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  • Atorvastatin preferentially inhibits the growth of high ZEB-expressing canine cancer cells. Reviewed International journal

    Takuro Ishikawa, Tomohiro Osaki, Akihiro Sugiura, Jiro Tashiro, Tomoko Warita, Yoshinao Z Hosaka, Katsuhiko Warita

    Veterinary and comparative oncology   20 ( 1 )   313 - 323   2021.10   ISSN:14765810

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    DOI: 10.1111/vco.12778

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  • Estrogen modulates the skeletal muscle regeneration process and myotube morphogenesis: morphological analysis in mice with a low estrogen status. Reviewed

    Rattanatrai Chaiyasing, Akihiro Sugiura, Takuro Ishikawa, Koichi Ojima, Katsuhiko Warita, Yoshinao Z Hosaka

    The Journal of veterinary medical science   83 ( 12 )   1812 - 1819   2021.10

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    DOI: 10.1292/jvms.21-0495

  • Inhibition of transient receptor potential vanilloid type 1 through α2 adrenergic receptors at peripheral nerve terminals relieves pain. Reviewed

    83 ( 10 )   1570 - 1581   2021.9

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    The activation of α2 adrenergic receptors contributes to analgesia not only in the central nervous system but also in the peripheral nervous system. We reported that noradrenaline inhibits the activity of transient receptor potential vanilloid 1 (TRPV1) evoked by capsaicin through α2 receptors in cultured rat dorsal root ganglion (DRG) neurons. However, it is unclear whether activation of TRPV1 expressed in peripheral nerve terminals is inhibited by α2 receptors and whether this phenomenon contributes to analgesia. Therefore, we examined effects of clonidine, an α2 receptor agonist, on several types of nociceptive behaviors, which may be caused by TRPV1 activity, and subtypes of α2 receptors expressed with TRPV1 in primary sensory neurons in rats. Capsaicin injected into hind paws evoked nociceptive behaviors and clonidine preinjected into the same site inhibited capsaicin-evoked responses. This inhibition was not observed when clonidine was injected into the contralateral hind paws. Preinjection of clonidine into the plantar surface of ipsilateral, but not contralateral, hind paws reduced the sensitivity to heat stimuli. Clonidine partially reduced formalin-evoked responses when it was preinjected into ipsilateral hind paws. The expression level of α2C receptor mRNA quantified by real-time PCR was highest followed by those of α2A and α2B receptors in DRGs. α2A andα2C receptor-like immunoreactivities were detected with TRPV1-like immunoreactivities in the same neurons. These results suggest that TRPV1 and α2 receptors are coexpressed in peripheral nerve terminals and that the functional association between these two molecules causes analgesia.

    DOI: 10.1292/jvms.21-0429

  • Concomitant attenuation of HMGCR expression and activity enhances the growth inhibitory effect of atorvastatin on TGF-β-treated epithelial cancer cells. Reviewed International journal

    Katsuhiko Warita, Takuro Ishikawa, Akihiro Sugiura, Jiro Tashiro, Hiroaki Shimakura, Yoshinao Z Hosaka, Ken-Ichi Ohta, Tomoko Warita, Zoltán N Oltvai

    11 ( 1 )   12763 - 12763   2021.6

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    Epithelial-mesenchymal transition (EMT) in primary tumor cells is a key prerequisite for metastasis initiation. Statins, cholesterol-lowering drugs, can delay metastasis formation in vivo and attenuate the growth and proliferation of tumor cells in vitro. The latter effect is stronger in tumor cells with a mesenchymal-like phenotype than in those with an epithelial one. However, the effect of statins on epithelial cancer cells treated with EMT-inducing growth factors such as transforming growth factor-β (TGF-β) remains unclear. Here, we examined the effect of atorvastatin on two epithelial cancer cell lines following TGF-β treatment. Atorvastatin-induced growth inhibition was stronger in TGF-β-treated cells than in cells not thusly treated. Moreover, treatment of cells with atorvastatin prior to TGF-β treatment enhanced this effect, which was further potentiated by the simultaneous reduction in the expression of the statin target enzyme, 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR). Dual pharmacological targeting of HMGCR can thus strongly inhibit the growth and proliferation of epithelial cancer cells treated with TGF-β and may also improve statin therapy-mediated attenuation of metastasis formation in vivo.

    DOI: 10.1038/s41598-021-91928-3

  • Absence of estrogen receptors delays myoregeneration and leads to intermuscular adipogenesis in a low estrogen status: morphological comparisons in estrogen receptor alpha and beta knock out mice. Reviewed

    Rattanatrai Chaiyasing, Takuro Ishikawa, Katsuhiko Warita, Yoshinao Z Hosaka

    The Journal of veterinary medical science   83 ( 7 )   1022 - 1030   2021.5

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    DOI: 10.1292/jvms.20-0696

  • Localization of uncoupling protein 1 (UCP-1) in the sebaceous gland of the dorsal region in the Sunda porcupine (Hystrix javanica). Reviewed

    Andhika Yudha Prawira, Savitri Novelina, Wartika Rosa Farida, Huda Shalahudin Darusman, Katsuhiko Warita, Srihadi Agungpriyono, Yoshinao Z Hosaka

    The Journal of veterinary medical science   82 ( 12 )   1729 - 1733   2020.12

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    DOI: 10.1292/jvms.20-0412

  • Effect of yogurt fermented by Lactobacillus fermentum Tsi and L. Fermentum S2 derived from a Mongolian traditional dairy product on rats with high-fat-diet-induced obesity Reviewed International journal

    Cho, W.-Y., Hong, G.-E., Lee, H.-J., Yeon, S.-J., Paik, H.-D., Hosaka, Y.Z., Lee, C.-H.

    Foods   9 ( 5 )   2020.5

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    DOI: 10.3390/foods9050594

  • Morphological evaluation of polysaccharide content and collagen composition during cutaneous wound healing in the Sunda porcupine (Hystrix javanica). Reviewed

    Andhika Yudha Prawira, Yoshinao Z Hosaka, Savitri Novelina, Wartika Rosa Farida, Huda Shalahudin Darusman, Srihadi Agungpriyono

    The Journal of veterinary medical science   82 ( 5 )   506 - 515   2020.5

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    DOI: 10.1292/jvms.19-0603

  • Neutralization of transforming growth factor (TGF)-β1 activity reduced fibrosis and enhanced regeneration of glycerol-injured rat muscle. Reviewed

    82 ( 2 )   168 - 171   2020.2

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    DOI: 10.1292/jvms.19-0446

  • Compositional analysis of the glycosaminoglycan family in velvet antlers of Sika deer (Cervus nippon) at different growing stages. Reviewed International journal

    Naoko Takeda-Okuda, Shuji Mizumoto, Zui Zhang, Soo-Ki Kim, Chi-Ho Lee, Byong-Tae Jeon, Yoshinao Z Hosaka, Kenji Kadomatsu, Shuhei Yamada, Jun-Ichi Tamura

    Glycoconjugate journal   36 ( 2 )   127 - 139   2019.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s10719-019-09859-1

  • The precursor osteoblast-like cell, MC3T3-E1 cell line, enhances sodium-calcium exchanger 1 (Ncx1) gene expression by stretch stimuli prior to osteoblast differentiation. Reviewed

    Katsuhiko Warita, Ryo Aoki, Naoki Kitamura, Izumi Shibuya, Yoshinao Z Hosaka

    The Journal of veterinary medical science   81 ( 4 )   508 - 512   2019.4

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    DOI: 10.1292/jvms.18-0766

  • Glycerol induces early fibrosis in regenerating rat skeletal muscle. Reviewed

    Mohamed A A Mahdy, Katsuhiko Warita, Yoshinao Z Hosaka

    The Journal of veterinary medical science   80 ( 11 )   1646 - 1649   2018.11

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    DOI: 10.1292/jvms.18-0328

  • Concomitant attenuation of HMG-CoA reductase expression potentiates the cancer cell growth-inhibitory effect of statins and expands their efficacy in tumor cells with epithelial characteristics. Reviewed International journal

    Oncotarget   9 ( 50 )   29304 - 29315   2018.6

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    DOI: 10.18632/oncotarget.25448

  • Processing and Thermal Response of Temperature-Sensitive-Gel(TSG)/Polymer Composites. International journal

    Jin Gong, Eiichi Hosaka, Kohei Sakai, Hiroshi Ito, Yoshikazu Shibata, Kosei Sato, Dai Nakanishi, Shinichiro Ishihara, Kazuhiro Hamada

    Polymers   10 ( 5 )   2018.5

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    DOI: 10.3390/polym10050486

  • Immunohistochemical localization of osteoblast activating peptide in the mouse kidney. Reviewed International journal

    Ahmed E Noreldin, Yaser Hosny Ali Elewa, Yasuhiro Kon, Katsuhiko Warita, Yoshinao Z Hosaka

    Acta histochemica   120 ( 4 )   323 - 328   2018.5

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    DOI: 10.1016/j.acthis.2018.03.001

  • Effects of transforming growth factor-β1 treatment on muscle regeneration and adipogenesis in glycerol-injured muscle. Reviewed International journal

    88 ( 11 )   1811 - 1819   2017.11

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    DOI: 10.1111/asj.12845

  • Early ultrastructural events of skeletal muscle damage following cardiotoxin-induced injury and glycerol-induced injury. Reviewed International journal

    Mohamed A A Mahdy, Katsuhiko Warita, Yoshinao Z Hosaka

    Micron (Oxford, England : 1993)   91   29 - 40   2016.12

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    DOI: 10.1016/j.micron.2016.09.009

  • Degree of Suppression of Mouse Myoblast Cell Line C₂C12 Differentiation Varies According to Chondroitin Sulfate Subtype. Reviewed International journal

    14 ( 10 )   2016.10

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    DOI: 10.3390/md14100193

  • Spatial distribution of osteoblast activating peptide in the rat stomach. Reviewed International journal

    Ahmed E Noreldin, Maina Sogabe, Yoshiaki Yamano, Masato Uehara, Mohamed A A Mahdy, Mohamed A Elnasharty, Ahmed Sayed-Ahmed, Katsuhiko Warita, Yoshinao Z Hosaka

    Acta histochemica   118 ( 2 )   109 - 17   2016.3

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    DOI: 10.1016/j.acthis.2015.12.001

  • Repair of segmental ulna defects using a β-TCP implant in combination with a heparan sulfate glycosaminoglycan variant. Reviewed

    28   193 - 204   2015.12

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    DOI: 10.1016/j.actbio.2015.09.008

  • Comparative study of muscle regeneration following cardiotoxin and glycerol injury. Reviewed International journal

    Mohamed A A Mahdy, Hsiao Yin Lei, Jun-Ichi Wakamatsu, Yoshinao Z Hosaka, Takanori Nishimura

    Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft   202   18 - 27   2015.11

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    DOI: 10.1016/j.aanat.2015.07.002

  • The shortened spinal cord in tetraodontiform fishes. Reviewed International journal

    Masato Uehara, Yoshinao Z Hosaka, Hiroyuki Doi, Harumi Sakai

    Journal of morphology   276 ( 3 )   290 - 300   2015.3

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    DOI: 10.1002/jmor.20338

  • Spatial relationships among the cellular tapetum, visual streak and rod density in dogs. Reviewed

    Yasuhiro Yamaue, Yoshinao Z Hosaka, Masato Uehara

    The Journal of veterinary medical science   77 ( 2 )   175 - 9   2015.2

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    DOI: 10.1292/jvms.14-0447

  • Full-length transient receptor potential vanilloid 1 channels mediate calcium signals and possibly contribute to osmoreception in vasopressin neurones in the rat supraoptic nucleus. Reviewed International journal

    Taiki Moriya, Rina Shibasaki, Tomohiko Kayano, Nami Takebuchi, Momoko Ichimura, Naoki Kitamura, Atsushi Asano, Yoshinao Z Hosaka, Oksana Forostyak, Alexei Verkhratsky, Govindan Dayanithi, Izumi Shibuya

    Cell calcium   57 ( 1 )   25 - 37   2015.1

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    DOI: 10.1016/j.ceca.2014.11.003

  • Fucosyl chondroitin sulfate from sea cucumber - chemical synthesis and structural analysis

    Jun-ichi Tamura, Haruna Tanaka, Ayumi Nakamura, Ryosuke Toita, Yoshinao Z. Hosaka, Ken-ichi Nagamine, Ayaka Himono, Naoko Takeda

    GLYCOBIOLOGY   24 ( 11 )   1108 - 1108   2014.11

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  • Macroscopic and histological variations in the cellular tapetum in dogs. Reviewed

    Yasuhiro Yamaue, Yoshinao Z Hosaka, Masato Uehara

    The Journal of veterinary medical science   76 ( 8 )   1099 - 103   2014.8

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    DOI: 10.1292/jvms.14-0132

  • Prenatal development of the eye tunics in the dromedary camel (Camelus dromedarius) Reviewed

    M. Abdo, Y. Z. Hosaka, A. Erasha, M. Nada, S. Ali, M. Uehara

    Journal of Veterinary Medicine Series C: Anatomia Histologia Embryologia   43 ( 4 )   257 - 264   2014.8

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    DOI: 10.1111/ahe.12069

  • Histological analysis of glucagon-like peptide-1 receptor expression in chicken pancreas. Reviewed International journal

    Takafumi Watanabe, Kei Nishimura, Yoshinao Z Hosaka, Takeshi Shimosato, Shinichi Yonekura, Daisuke Suzuki, Chihiro Takemoto, Mohammad Moniruzzaman Monir, Kohzy Hiramatsu

    Cell and tissue research   357 ( 1 )   55 - 61   2014.7

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    DOI: 10.1007/s00441-014-1836-6

  • Longitudinal projections of primary afferents from the single dorsal root ganglion of the cervical or lumbosacral enlargements in chickens. Reviewed International journal

    Masato Uehara, Yoshinao Z Hosaka

    Neuroscience letters   561   118 - 22   2014.2

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    DOI: 10.1016/j.neulet.2013.12.052

  • Diamond squid (Thysanoteuthis rhombus)-derived chondroitin sulfate stimulates bone healing within a rat calvarial defect. Reviewed International journal

    Yoshinao Z Hosaka, Yuji Iwai, Jun-ichi Tamura, Masato Uehara

    Marine drugs   11 ( 12 )   5024 - 35   2013.12

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    DOI: 10.3390/md11125024

  • The fibrous tapetum of the horse eye. Reviewed International journal

    Aya Shinozaki, Satoshi Takagi, Yoshinao Z Hosaka, Masato Uehara

    Journal of anatomy   223 ( 5 )   509 - 18   2013.11

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    DOI: 10.1111/joa.12100

  • Effectiveness of isoprothiolane in preventing fatty liver in dairy cows Reviewed

    Minaguchi, J, Miura, M, Oikawa, S, Handa, F, Haraguchi, T, Miyamoto-Ohno, K, Ito, Y, Hosaka, Y, Ueda, H, Takehana, K

    7   7 - 11   2013.1

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    Effectiveness of isoprothiolane in preventing fatty liver in dairy cows

  • Scanning electron microscopic study of the vascular system in the hemal node of the bovine cervical region. Reviewed

    Weidong Zhang, Masahiro Yasuda, Yoshinao Z Hosaka, Tetsuo Nasu

    The Journal of veterinary medical science   75 ( 1 )   79 - 83   2013.1

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    DOI: 10.1292/jvms.12-0156

  • Myostatin regulates proliferation and extracellular matrix mRNA expression in NIH3T3 fibroblasts. Reviewed

    Yoshinao Z Hosaka, Mika Ishibashi, Jun-Ichi Wakamatsu, Masato Uehara, Takanori Nishimura

    Biomedical research (Tokyo, Japan)   33 ( 6 )   355 - 61   2012.12

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    DOI: 10.2220/biomedres.33.355

  • Vasopressin-induced intracellular Ca²⁺ concentration responses in non-neuronal cells of the rat dorsal root ganglion. Reviewed International journal

    1483   1 - 12   2012.11

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    DOI: 10.1016/j.brainres.2012.08.028

  • Hepatocyte apoptosis in dairy cattle during the transition period. Reviewed International journal

    Mohamed Tharwat, Aya Takamizawa, Yoshinao Z Hosaka, Daiji Endoh, Shin Oikawa

    Canadian journal of veterinary research = Revue canadienne de recherche veterinaire   76 ( 4 )   241 - 7   2012.10

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  • Formation of excess sublobules in the cerebellum of hypothyroid rats. Reviewed International journal

    Yoshinao Z Hosaka, Yoshihiko Neki, Miki Hasebe, Aya Shinozaki, Masato Uehara

    Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft   194 ( 4 )   329 - 33   2012.7

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    DOI: 10.1016/j.aanat.2011.12.005

  • Comparative studies on the distribution and population of immunocompetent cells in bovine hemal node, lymph node and spleen. Reviewed

    Weidong Zhang, Tetsuo Nasu, Yoshinao Z Hosaka, Masahiro Yasuda

    The Journal of veterinary medical science   74 ( 4 )   405 - 11   2012.4

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    DOI: 10.1292/jvms.11-0405

  • Laterality of spinocerebellar neurons in the chicken spinal cord. Reviewed

    Masato Uehara, Masahide Akita, Masato Furue, Aya Shinozaki, Yoshinao Z Hosaka

    The Journal of veterinary medical science   74 ( 4 )   495 - 8   2012.4

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    DOI: 10.1292/jvms.11-0398

  • The complete control of murine pregnancy from embryo implantation to parturition. Reviewed International journal

    Jumpei Terakawa, Takaho Watanabe, Rutsuko Obara, Makoto Sugiyama, Naoko Inoue, Yasushige Ohmori, Yoshinao Z Hosaka, Eiichi Hondo

    Reproduction (Cambridge, England)   143 ( 3 )   411 - 5   2012.3

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    DOI: 10.1530/REP-11-0288

  • Concerted and adaptive alignment of decorin dermatan sulfate filaments in the graded organization of collagen fibrils in the equine superficial digital flexor tendon Reviewed International journal

    Takafumi Watanabe, Yasutada Imamura, Daisuke Suzuki, Yoshinao Hosaka, Hiromi Ueda, Kohzy Hiramatsu, Kazushige Takehana

    Journal of Anatomy   220 ( 2 )   156 - 163   2012.2

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    DOI: 10.1111/j.1469-7580.2011.01456.x

  • Efficacy of self-assembled hydrogels composed of positively or negatively charged peptides as scaffolds for cell culture. Reviewed International journal

    Aya Nagayasu, Hidenori Yokoi, Jun A Minaguchi, Yoshinao Z Hosaka, Hiromi Ueda, Kazushige Takehana

    Journal of biomaterials applications   26 ( 6 )   651 - 65   2012.2

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    DOI: 10.1177/0885328210379927

  • Embryo implantation is blocked by intraperitoneal injection with anti-LIF antibody in mice. Reviewed

    Jumpei Terakawa, Shoichi Wakitani, Makoto Sugiyama, Naoko Inoue, Yasushige Ohmori, Yasuo Kiso, Yoshinao Z Hosaka, Eiichi Hondo

    The Journal of reproduction and development   57 ( 6 )   700 - 7   2011.12

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    DOI: 10.1262/jrd.11-048h

  • VASOPRESSIN-INDUCED [CA(2+)]I INCREASE IN NON-NEURONAL CELLS FROM RAT DORSAL ROOT GANGLIA

    G. Dayanithi, T. Moriya, T. Kayano, N. Kitamura, Y. Hosaka, C. Viero, E. C. Toescu, O. Forostyak, Shibuya, I

    GLIA   59   S105 - S105   2011.10

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  • Embryo implantation is blocked by intraperitoneal injection with anti-LIF antibody in mice Reviewed

    Jumpei Terakawa, Shoichi Wakitani, Makoto Sugiyama, Naoko Inoue, Yasushige Ohmori, Yasuo Kiso, Yoshinao Z. Hosaka, Eiichi Hondo

    Journal of Reproduction and Development   57 ( 6 )   700 - 707   2011.8

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    DOI: 10.1262/jrd.11-048H

  • Expression of small leucine-rich proteoglycans in the developing retina and kainic acid-induced retinopathy in ICR mice. Reviewed

    Safwat Ali Mohamed Ali, Yoshinao Z Hosaka, Masato Uehara

    The Journal of veterinary medical science   73 ( 4 )   439 - 45   2011.4

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    DOI: 10.1292/jvms.10-0464

  • Constitution of the ependyma in the chicken telencephalon. Reviewed

    Shinsuke Uchida, Tomohiro Imagawa, Masato Furue, Safwat Ali Mohamed Ali, Yoshinao Z Hosaka, Masato Uehara

    The Journal of veterinary medical science   73 ( 3 )   319 - 23   2011.3

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    DOI: 10.1292/jvms.10-0377

  • Expressions of apoptosis-regulating factors in bovine retained placenta. Reviewed International journal

    Y Kamemori, K Wakamiya, R Nishimura, Y Hosaka, S Ohtani, K Okuda

    Placenta   32 ( 1 )   20 - 6   2011.1

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    DOI: 10.1016/j.placenta.2010.10.016

  • Spatiotemporal distribution of chondroitin sulfate proteoglycans in the developing mouse retina and optic nerve. Reviewed

    Safwat Ali Mohamed Ali, Yoshinao Z Hosaka, Masato Uehara

    The Journal of veterinary medical science   73 ( 1 )   13 - 8   2011.1

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    DOI: 10.1292/jvms.10-0201

  • Distribution of Astroglial Lineage Cells in Developing Chicken Telencephalon from Embryo to Young Chick Reviewed

    Shinsuke Uchida, Tomohiro Imagawa, Aya Shinozaki, Masato Furue, Safwat Ali, Yoshinao Hosaka, Masato Uehara

    JOURNAL OF VETERINARY MEDICAL SCIENCE   72 ( 12 )   1597 - 1602   2010.12

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    DOI: 10.1292/jvms.10-0209

  • Morphological and biochemical analysis of intact and opaque cornea in dogs. Reviewed

    Masamitsu Hirooka, Osamu Igarashi, Aya Nagayasu, Jun Minaguchi, Yoshinao Z Hosaka, Hiromi Ueda, Prasarn Tangkawattana, Kazushige Takehana

    Okajimas folia anatomica Japonica   87 ( 2 )   59 - 64   2010.8

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    DOI: 10.2535/ofaj.87.59

  • Topography of Ganglion Cells and Photoreceptors in the Sheep Retina Reviewed International journal

    Aya Shinozaki, Yoshinao Hosaka, Tomohiro Imagawa, Masato Uehara

    JOURNAL OF COMPARATIVE NEUROLOGY   518 ( 12 )   2305 - 2315   2010.6

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    DOI: 10.1002/cne.22333

  • Comparative study of the characteristics and properties of tendinocytes derived from three tendons in the equine forelimb. Reviewed International journal

    Yoshinao Z Hosaka, Hideyuki Takahashi, Takehiro Uratsuji, Prasarn Tangkawattana, Hiromi Ueda, Kazushige Takehana

    Tissue & cell   42 ( 1 )   9 - 17   2010.2

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    DOI: 10.1016/j.tice.2009.06.002

  • Relationship between Distribution of Tapetum Fibrosum and Retinal Pigment Epithelium in the Sheep Eye Reviewed

    Aya Shinozaki, Yoshinao Hosaka, Tomohiro Imagawa, Masato Uehara

    JOURNAL OF VETERINARY MEDICAL SCIENCE   72 ( 2 )   211 - 215   2010.2

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    DOI: 10.1292/jvms.09-0413

  • Comparative study of the properties of tendinocytes derived from three different sites in the equine superficial digital flexor tendon. Reviewed

    Yoshinao Z Hosaka, Takehiro Uratsuji, Hiromi Ueda, Masato Uehara, Kazushige Takehana

    Biomedical research (Tokyo, Japan)   31 ( 1 )   35 - 44   2010.2

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    DOI: 10.2220/biomedres.31.35

  • 飼料成分の違いによる空腸絨毛の形態学的変化 Reviewed

    星野 佑治, 玉置 和之, 稲山 一成, 上野 光敏, 永易 彩, 保坂 善真, 植田 弘美, 竹花 一成

    日本畜産学会報 = The Japanese journal of zootechnical science   80 ( 2 )   171 - 177   2009.5

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    飼料成分の違いによる仔豚空腸絨毛の変化を,形態学的手法を用いて評価した。同腹7頭の仔豚のうち,離乳直後1頭を無処理群とし,3頭を乳製品原料主体飼料給餌群(乳製品飼料群),3頭を植物原料主体飼料給餌群(植物飼料群)に区分し用いた。離乳する6日前から餌付けを行い,離乳後,乳製品飼料群には乳製品を主体とした飼料を,植物飼料群には植物原料を主体とした飼料をそれぞれ給餌し,飲水は自由とした。体重増加割合は離乳3日後および14日後で,乳製品飼料群よりも植物飼料群の方が高かった。絨毛長は離乳3日後,吸収上皮細胞の大きさは離乳14日後,微絨毛長は離乳3日後および14日後に,いずれも乳製品飼料群に比べ植物飼料群で有意に増大していた。また,離乳3日後において乳製品飼料群に比べ植物飼料群では吸収上皮細胞の剥離はほとんど観察されなかった。その要因としては,乳製品飼料群よりも植物飼料群に与えた飼料の方が消化され易い成分構成であったことが考えられ,それ故に絨毛や吸収上皮細胞の成長が促進されたものと思われる。以上の結果より,離乳後に植物主体の飼料を与えることによって仔豚の空腸絨毛の成長が促進され,吸収面積が増大して栄養素の吸収効率が向上したことが示唆された。 The aim of this study was to evaluate morphological responses of the absorptive apparatus of the jejunum to di erent diet ingredients in weaning pigs. In this study, seven crossbred (Landrace×Large Yorkshire×Duroc) pigs, they were a litter, were used after weaning. Before weaning, they fed with creep feed for six days. Six pigs were divided into two groups ; one received dairy-based feed and the other received vegetable-based feed. The remaining pig was used as a non-treatment control. Dairy-based feed contained mainly animal-based feed, and vegetable-based feed contained mainly oil-seed cake and meal. Drinking water was provided ad libitum throughout the experimental period. Jejunum samples were collected immediately after weaning from a non-treated control pig (D0). The similar samples were collected from each 3 pigs of dairy-based feed and vegetable-based feed groups respectively, on 3 (D3) and 14 days (D14) after weaning. The samples were processed for morphological observation of absorptive epithelial cells and heights of villi and microvilli. A significant increase in height of intestinal villi on D3 and in size of the absorptive cells on D14 were found in animals that received vegetable-based feed , ultrastructural transition of epithelial cells on D3 . An increases in microvilli height on D3 and D14 were seen in animals that received vegetable-based feed. Presumably, the ingredients of vegetable-based feed are more easily digested and absorbed through the intestinal wall than are ingredients of dairy-based feed. As a result, the growth of intestinal villi and (growth of) absorptive epithelial cells in vegetable-based feed group were greater than those in dairy-based feed group, leading to a more e cient absorptive function of the small intestine.

    DOI: 10.2508/chikusan.80.171

  • Efficacy of antivascular photodynamic therapy using benzoporphyrin derivative monoacid ring A (BPD-MA) in 14 dogs with oral and nasal tumors

    Osaki Tomohiro, Takagi Satoshi, Hoshino Yuki, OKUMURA Masahiro, KADOSAWA Tsuyoshi, FUJINAGA Toru

    The journal of veterinary medical science   71 ( 2 )   125 - 132   2009.2

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    Efficacy of Antivascular Photodynamic Therapy Using Benzoporphyrin Derivative Monoacid Ring A (BPD-MA) in 14 Dogs with Oral and Nasal Tumors
    Antivascular photodynamic therapy (PDT) Suppresses tumor growth and prolonged the survival in solid tumor-bearing mice. The purpose of this study was to assess the efficacy of antivascular PDT using BPD-MA for treatment of oral and nasal tumors in 14 dogs. At 15 min after initiating intravenous infusion of 0.5 mg/kg benzoporphyrin derivative monoacid ring A, tumors were irradiated with laser light at 690 nm emitted by a diode laser, The 1-year survival rate of 7 dogs with oral tumors was 71%. The 1-year Survival rate of 7 does with nasal tumors was 57%. Imaging of each tumor was performed by using angiographic computed tomography before and after each antivascular PDT. Contrast-enhanced turners were observed before antivascular PDT, but these tumors were not enhanced with contrast medium following antivascular PDT. Antivascular PDT is suggested to be a promising method for dogs with oral and nasal tumors that cannot be effectively treated with current antitumor therapies.

    DOI: 10.1292/jvms.71.125

  • Effect of Collagen Oligopeptide Injection on Rabbit Tenositis Reviewed

    Ueda Hiromi, Meguri Natsuko, Minaguchi Jun, WATANABE Takafumi, NAGAYASU Aya, HOSAKA Yoshinao, TANGKAWATTANA Prasarn, KOKAI Yasuo, TAKEHANA Kazushige

    The journal of veterinary medical science   70 ( 12 )   1295 - 1300   2008.12

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    Effect of Collagen Oligopeptide Injection on Rabbit Tenositis
    Effects of the collagen oligopeptide (COP) were examined by its repeat injection into the inflammatory rabbit Achilles tendon (Tendo calcaneus communis), in which tenositis was induced by injection of bacterial collagenase. COP was evaluated 5 times over a period of 3 weeks to I month after injection of collagenase. At I month after treatment, the therapeutic effect of COP was evaluated by examining the structure of collagen fibrils, amount and components of glycosaminoglycans (GAGS) and matrix metalloproteinases (MMPs), and compared with the saline injection, control, and normal groups. The Achilles tendon of rabbit in the control group (no COP injection) and saline injection group showed a notable increase in the number of fine collagen fibrils, a change in GAG composition and increase in the amount of pro-MMP-2, indicating the weakening of the tendon. In contrast, the size distribution of collagen fibrils, GAG composition and the amount of pro-NIMP-2 was similar to those in the normal group. These results suggest that COP injection promotes healing processes of the tendon injury.

    DOI: 10.1292/jvms.70.1295

  • 内毒素血症に関与したウマの疝痛における腸間膜動脈の形態学的研究 Reviewed

    日高 修平, 植田 弘美, 保坂 善真, 竹花 一成

    酪農学園大学紀要 自然科学編   33 ( 1 )   99 - 104   2008.10

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    ウマにおいて疝痛を伴った内毒素血症はしばしば発症する疾病であって、毒素によって腸間膜動脈には様々な程度の虚血、動脈の痙攣や壊死が引き起こされる。重篤な場合では、それらの臨床症状が疝痛の前駆的発症要因になることが報告されている。エンドトキシンが関与し疝痛を患ったウマでは、腸間膜の血管における障害はこれまでのところ大いに注目されてきている。そこで、この研究では内毒素血症を自然発症したサラブレットにおいて、腸間膜動脈の障害を形態学的に検索した。この研究で得られた結果は、腸間膜にみられる血管の組織構造に対するエンドトキシンの効果をより良く理解できるものと思われる。試料は3.0%グルタルアルデヒド溶液に固定後、Quetol 812に包埋した。包埋した電顕試料より厚切り切片および超薄切片を作製し、前者には1%トルイジンブルー染色を施した後、光学顕微鏡を用いて、後者には酢酸ウラニウムとクエン酸鉛による二重染色を行った後、透過型電子顕微鏡にて詳細に検索した。発症群では動脈壁において血管内皮細胞の剥離、出血像、歪な形を呈した中膜平滑筋細胞や融解壊死した顆粒球などの血液細胞、空胞などが多数観察された。これらの結果は、すでに報告されているウマの内毒素血症の組織像と類似しており、また、エンドトキシンにより腸間膜中の動脈が損傷を受け、ウマの腸管において臓器性Shwartzman反応が起こったものと示唆された。

  • Morphological analysis of corneal opacity in Shiba dog with GM1 gangliosidosis Reviewed

    Nagayasu Aya, Nakamura Taito, Yamato Osamu, TSUZUKI Keiko, HOSAKA Yoshinao, UEDA Hiromi, TANGKAWATTANA Prasarn, TAKEHANA Kazushige

    The journal of veterinary medical science   70 ( 9 )   881 - 886   2008.9

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    Morphological Analysis of Corneal Opacity in Shiba Dog with GM1 Gangliosidosis
    GM1 gangliosidosis is one of the inherited metabolic lysosomal storage disorders characterized by neurological symptoms caused by beta-galactosidase deficiency and consequent accumulation of GM1 ganglioside in neuronal cells. Shiba dogs affected with GM1 gangliosidosis have been found to suffer from corneal opacity. In our morphological analysis, keratocyte enlargement was induced by abnormal intracellular accumulation of neutral carbohydrates, resulting in the loss of normal arrangement of collagen fibrils in the opaque cornea was found to be associated with the disorder. We therefore conclude that corneal opacity in this Shiba dog with GM1 gangliosidosis may be caused by neutral carbohydrate accumulation in lysosomes, swelling and dysfunction of keratocytes, and subsequent irregular arrangement of collagen fibrils in the corneal proper substance.

    DOI: 10.1292/jvms.70.881

  • The modulation of collagen fibril assembly and its structure by decorin : An electron microscopic study Reviewed

    IWASAKI Shunsuke, HOSAKA Yoshinao, IWASAKI Tomohito, YAMAMOTO Katsuhiro, NAGAYASU Aya, UEDA Hiromi, KOKAI Yasuo, TAKEHANA Kazushige

    Archives of histology and cytology   71 ( 1 )   37 - 44   2008.5

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    The modulation of collagen fibril assembly and its structure by decorin: An electron microscopic study
    The present study was carried out to determine the effect of decorin in the process of collagen assembly. Collagen fibrils were obtained in vitro by aggregation from commercialized acid-soluble type I collagen with the addition of different concentrations of decorin (0-25 mu g/ ml). All specimens were observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The distribution of collagen fibril diameters was also analyzed by TEM. In samples without or with low concentrations of decorin, highly porous collagen fiber networks were formed. On the other hand, dense networks were observed in samples treated with high concentrations of decorin. The influence of decorin secreted by cells on collagen fibrils was observed by SEM, and the fiber network elasticity was measured using a rheometer. SEM images showed that collagen fiber networks without fibroblasts were much looser than those cultured with normal fibroblasts. The networks cultured with the fibroblasts were composed of straight fibers with large diameters. On the other hand, collagen fiber networks cultured with siRNA-decorin-transfected (siDT) fibroblasts had loose, meandering fibers with small diameters. The elasticity of collagen fiber networks cultured with untransfected fibroblasts showed no significant difference over the 7-day incubation period. However, significantly lower elastic values were obtained for coliagen fiber networks treated with siDT cells on days 3 and 7. In addition, after treatment with 5.0 or 25 mu g/ml decorin, the collagen fiber networks cultured with siDT cells exhibited an altered structure that showed a dense structure similar to that of the fiber networks cultured with untransfected fibroblasts. In conclusion, this in vitro study showed that decorin is a regulatory and architecturally small leucinerich repeat proteoglycan in the process of collagen fibril assembly.

    DOI: 10.1679/aohc.71.37

  • Characteristics of collagen fibrils in the entire equine superficial digital flexor tendon. Reviewed

    Masaru Sese, Hiromi Ueda, Takafumi Watanabe, Etsuko Yamamoto, Yoshinao Hosaka, Prasarn Tangkawattana, Kazushige Takehana

    Okajimas folia anatomica Japonica   84 ( 3 )   111 - 4   2007.11

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    DOI: 10.2535/ofaj.84.111

  • ウマ浅指屈筋腱炎内の肉芽組織におけるMMP-13 mRNA発現 Reviewed

    野村 正幸, 保坂 善真, 笠嶋 快周, 植田 弘美, 竹花 一成, 桑野 睦敏, 新井 克彦

    69 ( 6 )   637 - 9   2007.6

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    ウマ浅指屈筋腱炎における細胞外マトリックス遺伝子発現の特徴を把握するため、DNAマイクロアレイ解析を実施したところ、屈腱炎組織においてMMP-13 mRNAの発現上昇が見られた。In situハイブリダイゼーションにより、屈腱炎内で増生した肉芽組織中の線維芽細胞様の細胞に強い陽性シグナルが確認されたが、健常腱組織では、その陽性細胞は腱内膜内にわずかに見られたのみであった。このことから、屈腱炎におけるMMP-13の重要な役割が示唆された。

    DOI: 10.1292/jvms.69.637

  • Antitumor effects and blood flow dynamics after photodynamic therapy using benzoporphyrin derivative monoacid ring A in KLN205 and LM8 mouse tumor models International journal

    Osaki Tomohiro, Takagi Satoshi, Hoshino Yuki, Okumura Masahiro, Fujinaga Toru

    Cancer Letters   248 ( 1 )   47 - 57   2007.4

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    Antitumor effects and blood flow dynamics after photodynamic therapy using benzoporphyrin derivative monoacid ring A in KLN205 and LM8 mouse tumor models
    Photodynamic therapy (PDT) using benzoporphyrin derivative monoacid ring A (BPD-MA) induces direct tumor cell damage and microvascular injury. We administered BPD-MA at 3 h or 15 min before laser irradiation to KLN205 and LM8 tumors in murine models. Tumor growth delay was induced more effectively by 15-min-interval PDT than by 3-h-interval PDT. Vascularity and blood perfusion was significantly decreased by 15-min-interval PDT. We observed death of all tumor cells, except peripheral cells, in the 3-h-interval PDT group, and death of cells around the damaged tumor vasculature in the 15-min-interval PDT group. Thus, 15-min-interval PDT enhanced the antitumor effect by damaging tumor vasculature.

  • Intranuclear paracrystals observed in striated muscle specific LIM protein-deficient mouse cardiomyocytes Reviewed

    M. Muto, A. Sargeant, P. Tangkawattana, K. Takeyasu, Y. Nishijima, J. Bonagura, M. Hoshijima, T. Nakade, Y. Hosaka, G. Faulkner, K. Tomono, M. Yamaguchi

    MICROSCOPY RESEARCH AND TECHNIQUE   70 ( 1 )   50 - 54   2007.1

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    DOI: 10.1002/jemt.20386

  • Effect of heat on synthesis of gelatinases and pro-inflammatory cytokines in equine tendinocytes Reviewed

    Yoshinao Hosaka, Sachiko Ozoe, Rikio Kirisawa, Hiromi Ueda, Kazushige Takehana, Mamoru Yamaguchi

    BIOMEDICAL RESEARCH-TOKYO   27 ( 5 )   233 - 241   2006.10

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    DOI: 10.2220/biomedres.27.233

  • Effects of Ingestion of Collagen Peptide on Collagen Fibrils and Glycosaminoglycans in the Dermis Reviewed

    Matsuda Naoya, Koyama Yoh-ichi, Hosaka Yoshinao, UEDA Hiromi, WATANABE Takafumi, ARAYA Takayuki, IRIE Shinkichi, TAKEHANA Kazushige

    Journal of nutritional science and vitaminology   52 ( 3 )   211 - 215   2006.6

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    Effects of ingestion of collagen peptide on collagen fibrils and Glycosaminoglycans in the dermis
    In order to investigate the effects of collagen peptide ingestion on fibroblasts and the extracellular matrix in the dermis, collagen peptide was administered orally to pigs at 0.2 g/kg body weight/d for 62 d, and its effects were compared with those of lactalbumin and water controls. Fibroblast density, and diameter and density of collagen fibrils were significantly larger in the collagen peptide group than in the lactalbumin and water control groups. The two major components of dermal glycosaminoglycans, hyaluronic acid and dermatan sulfate, which are present in the inter-fibrillar space, did not differ significantly among the three groups. However, the ratio of dermatan sulfate, which is derived from fibril-bound decorin, was largest in the collagen peptide group. These results suggest that ingestion of collagen peptide induces increased fibroblast density and enhances formation of collagen fibrils in the dermis in a protein-specific manner.

    DOI: 10.3177/jnsv.52.211

  • Morphological, biochemical and mechanical features of the cranial cruciate and lateral collateral ligaments in dogs. Reviewed

    Hiromi Ueda, Takuya Matsukawa, Takafumi Watanabe, Yoshinao Hosaka, Kazushige Takehana

    Okajimas folia anatomica Japonica   83 ( 1 )   25 - 31   2006.5

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    DOI: 10.2535/ofaj.83.25

  • Structure and component alteration of rabbit Achilles tendon in tissue culture

    Yoshinao Hosaka, Hiromi Ueda, Tadatsugu Yamasaki, Daisuke Suzuki, Naoya Matsuda, Kazushige Takehana

    BIOMEDICAL RESEARCH-TOKYO   26 ( 6 )   279 - 286   2005.12

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    DOI: 10.2220/biomedres.26.279

  • Differences in tumor necrosis factor (TNF)alpha and TNF receptor-1-mediated intracellular signaling factors in normal, inflamed and scar-formed horse tendons. Reviewed

    The Journal of Veterinary Medical Science   67 ( 10 )   985 - 91   2005.10

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    Differences in tumor necrosis factor (TNF)alpha and TNF receptor-1-mediated intracellular signaling factors in normal, inflamed and scar-formed horse tendons.
    Tumor necrosis factor (TNF) receptors (TNF-R)-mediated cell survival or apoptosis has been demonstrated in many cells, but little is known about survival or apoptotic signals via TNF-R1 in tendinocytes. In this study, we focused on four signaling factors, TNFalpha, TNF-R1, TNFR-associated factor2 (TRAF2) and caspase-3, in order to elucidate the signaling events in tendinocytes. Samples were obtained from normal, inflamed and scar-formed equine superficial digital flexor tendons. To detect these signaling factors, samples were subjected to immunohistochemistry and Western blot analysis, and some samples were also subjected to reverse transcription-polymerase chain reaction (RT-PCR), PCR-Southern blot analysis and in situ hybridization to detect the expression of TNFalpha mRNA. Distribution of the four factors differed depending on the tendon condition, normal, inflamed or scar-formed. In the normal tendon, large amounts of TRAF2 were found in tendinocytes, but the amounts of TNF-R1 were small. TNFalpha mRNA was expressed most highly in the inflamed tendon. TNF-R1, which was only faintly detected in the normal tendon, was detected at a high level in the inflamed tendon, and the amounts of TRAF2 and caspase-3 also increased. Activated caspase-3 was only detected in the inflamed tendon. TNFalpha mRNA was also expressed in the scar-formed tendon, though it showed weak signals, and the expression levels of TNF-R1, TRAF2 and caspase-3 proteins were very low. Two distinct intracellular signaling pathways of TNFalpha, which lead to cell survival and apoptosis, might be present in tendinocytes mediated through TNF-R1. These results, which reflect the dynamism of TNFalpha, provide important clues for means to prevent tendinopathy.

    DOI: 10.1292/jvms.67.985

  • Differences in tumor necrosis factor (TNF) α and TNF receptor-1-mediated intracellular signaling factors in normal, inflamed and scar-formed horse tendons.

    67 ( 10 )   985 - 991   2005.10

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    DOI: 10.1292/jvms.67.985

  • Functional ability of tendinocytes to take up Fe substances in an inflamed tendon. Reviewed

    Yoshinao Hosaka, Mamoru Yamaguchi, Kazushige Takehana

    Archives of histology and cytology   68 ( 3 )   161 - 9   2005.9

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    DOI: 10.1679/aohc.68.161

  • Effects of Ingestion of Collagen Peptide on Collagen Fibrils and Glycosaminoglycans in Achilles Tendon

    Minaguchi Jun, Koyama Yoh-ichi, Meguri Natsuko, HOSAKA Yoshinao, UEDA Hiromi, KUSUBATA Masashi, HIROTA Arisa, IRIE Shinkichi, MAFUNE Naoki, TAKEHANA Kazushige

    Journal of nutritional science and vitaminology   51 ( 3 )   169 - 174   2005.6

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    Effects of Ingestion of Collagen Peptide on Collagen Fibrils and Glycosaminoglycans in Achilles Tendon
    In order to investigate whether the oral ingestion of collagen peptide affects the extracellular matrix of tendon, two doses (0.2g/kg and 1.0g/kg body weight) were orally administered daily for 56d to a rabbit, and both the size of collagen fibrils and the amount of glycosaminoglycans in the Achilles tendon were measured in comparison with those in a rabbit fed with a control protein, lactalbumin, or water alone. Ingestion of col-lagen peptide or lactalbumin induced a significant increase in collagen fibril diameter and a decrease in fibril density except for a high dose of lactalbumin compared with the water con-trol. A histogram pattern of fibril diameter in a high dose of collagen peptide showed a peak at 160-180nm, which was not observed in other groups. However the percentage of diam-eters over 200nm was the lowest in this group but highest in the low-dose group of collagen peptide. The mean fibril diameter and mass average diameter of a high dose of collagen pep-tide were significantly smaller than those in a low dose. The amount of dermatan sulphate increased in the high-dose groups, while the amount of hyaluronic acid decreased in rabbits fed with collagen peptide or lactalbumin at either dose. These results suggest that the inges-tion of collagen peptide affects the size of collagen fibrils and composition of glycosaminogly-cans in the Achilles tendon and thus may improve the mechanical properties of the Achilles tendon.

    DOI: 10.3177/jnsv.51.169

  • Molecular cloning of canine membrane-anchored inhibitor of matrix metalloproteinase, RECK.

    Satoshi Takagi, Takanori Kitamura, Yoshinao Hosaka, Tomohiro Ohsaki, Darko Bosnakovski, Tsuyoshi Kadosawa, Masahiro Okumura, Toru Fujinaga

    The Journal of veterinary medical science   67 ( 4 )   385 - 91   2005.4

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    DOI: 10.1292/jvms.67.385

  • Downregulation of Decorin and Transforming Growth Factor-β1 by Decorin Gene Suppression in Tendinocytes International journal

    46 ( 1 )   18 - 26   2005.2

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    Scars formed after tendonitis result in altered tissue mechanical properties after injury. The interaction of collagen molecules with decorin affects collagen fibrogenesis, and scar tissue is fragile as a consequence of a large amount of decorin in the scar. We hypothesized that scar formation could be prevented by controlling decorin expression in tendinocytes. As a preliminary experiment, we treated tendinocytes with decorin antisense oligodeoxynucleotides (ODNs). Tendinocytes were isolated from Achilles tendons of New Zealand white rabbits and treated with ODN. When tendinocytes were transfected with decorin sense ODN, there was no alteration, whereas decorin antisense ODN-treated tendinocytes showed suppression of transforming growth factor (TGF)-β1 production. Decorin and TGF-β1-production of tendinocytes is regulated by decorin gene suppression. The results showed that the antisense approach is an attractive therapeutic strategy not only for preventing decorin deposition in scar tissue, which decreases collagen fibril diameter, but also for controlling TGF-β1 production, which leads to organ fibrosis. Copyright © Taylor & Francis Inc.

    DOI: 10.1080/03008200590935510

  • Mechanism of cell death in inflamed superficial digital flexor tendon in the horse

    Y Hosaka, H Teraoka, E Yamamoto, H Ueda, K Takehana

    JOURNAL OF COMPARATIVE PATHOLOGY   132 ( 1 )   51 - 58   2005.1

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    DOI: 10.1016/j.jcpa.2004.06.006

  • Distribution of TNF receptors and TNF receptor-associated intracellular signaling factors on equine tendinocytes in vitro. Reviewed

    Yoshinao Hosaka, Yurie Sakamoto, Rikio Kirisawa, Takafumi Watanabe, Hiromi Ueda, Kazushige Takehana, Mamoru Yamaguchi

    The Japanese journal of veterinary research   52 ( 3 )   135 - 44   2004.11

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    DOI: 10.14943/jjvr.52.3.135

  • Morphological study of the Golgi tendon organ in equine superficial digital flexor tendon.

    Takafumi Watanabe, Yoshinao Hosaka, Etsuko Yamamoto, Hiromi Ueda, Prasarn Tangkawattana, Kazushige Takehana

    Okajimas folia anatomica Japonica   81 ( 2-3 )   33 - 7   2004.8

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    DOI: 10.2535/ofaj.81.33

  • Ultrastructure and function of Sertoli cell processes in the Korean native goat.

    Geon Oh Kwon, Young Seok Park, Seong Ho Lee, Etsuko Yamamoto, Yoshinao Hosaka, Hiromi Ueda, Kazushige Takehana

    Okajimas folia anatomica Japonica   79 ( 4 )   121 - 7   2002.10

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    DOI: 10.2535/ofaj.79.121

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Books

  • 臨床獣医 2024年5月号 イラストで学ぶ牛の解剖生理学 第2回 「雄の生殖器の解剖学」

    著者:昆 泰寛 監修:保坂善真( Role: Supervisor (editorial))

    緑書房  2024.5 

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    Language:Japanese   Book type:Scholarly book

  • 臨床獣医 2024年4月号 イラストで学ぶ牛の解剖生理学 第1回 「尿生殖器系の発生」

    著者:昆 泰寛 監修:保坂善真( Role: Supervisor (editorial))

    緑書房  2024.4 

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    Language:Japanese   Book type:Scholarly book

  • 獣医組織学 第九版

    日本獣医解剖学会( Role: Joint author)

    2022.3 

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    Language:Japanese   Book type:Scholarly book

  • 獣医組織学 第八版

    日本獣医解剖学会( Role: Joint author)

    学窓社  2017.3 

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    Responsible for pages:総ページ数:xiv, 362p   Language:Japanese   Book type:Scholarly book

  • カラーアトラス獣医解剖学

    König, Horst Erich, Liebich, Hans-Georg, カラーアトラス獣医解剖学編集委員会

    チクサン出版社,緑書房 (発売)  2010.3 

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    Responsible for pages:総ページ数:2冊   Language:Japanese  

Presentations

  • 性ホルモンがつかさどる骨格筋の形成・維持のメカニズム Invited

    保坂善真

    中日学術交流会  2023.10 

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    Event date: 2024.10

    Language:English   Presentation type:Oral presentation (general)  

    Venue:大連理工大学   Country:China  

  • 低エストロゲン状態下での骨格筋再生の特徴 Invited

    保坂 善真

    酪農学園大学大学院特別セミナー  2023.10 

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    Event date: 2023.10

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    Venue:酪農学園大学   Country:Japan  

  • 雌性ホルモンエストロゲンによる骨格筋の量的・質的制御 Invited

    保坂 善真

    日本畜産学会第131会大会 シンポジウム 骨格筋の量と質を制御する分子機構と畜産学への応用  2023.9 

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    Event date: 2023.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:帯広畜産大学   Country:Japan  

  • 筋芽細胞による自律的な筋線維型制御における機械的伸展刺激の影響

    中田 后紀、川崎 佑華、大山 竜輝、前野 岳大、今給黎 燿、小川 陽平、大崎 莉果、小川 蒼生、保坂 善真、中村 真子、辰巳 隆一、鈴木 貴弘

    日本畜産学会第131回大会  2023.9 

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    Event date: 2023.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:帯広畜産大学   Country:Japan  

  • Adipose tissue properties in the low estrogen state and high fat diet-induced obesity mice

    2023.9 

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    Event date: 2023.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • Quantitative and qualitative analysis of chondroitin/dermatan sulfate in 3T3-L1 cells before and after induction of adipogenesis International conference

    Danang Dwi Cahyadi, Katsuhiko Warita, Naoko Takeda-Okuda, Jun-ichi Tamura, @Yoshinao Z. Hosaka

    7th AJIVE Symposium, 2023  2023.2 

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    Event date: 2023.2

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Indonesia  

  • Adopose tissue properties in the estrogen state and high fat diet-indued obesity mouse

    2022.10 

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    Event date: 2022.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  • 脱細胞化スキャフォールドの損傷筋組織での機能評価

    小倉裕平, 割田克彦, 保坂善真

    日本獣医学会学術集会講演要旨集  2020 

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  • 筋再生過程で特徴的なコンドロイチン硫酸合成および同分解酵素の発現と産生細胞の同定

    保坂善真, 保坂善真, 保坂善真, 鷲江壮太, CHAIYASING Rattanatrai, 割田克彦, 割田克彦, 割田克彦

    日本解剖学会総会・全国学術集会講演プログラム・抄録集  2020 

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  • 岐阜大学・鳥取大学共同獣医学科で実施したカリキュラム改定の趣旨

    鈴木正嗣, 保坂善真

    日本獣医学会学術集会講演要旨集  2020 

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  • Analytical study on chondroitin sulfate and dermatan sulfate in the cartilaginous tissues and internal organs of wild sika deer

    武田(奥田)尚子, YEON Su-Jung, 保坂善真, 田村純一

    日本糖質学会年会要旨集  2020 

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  • Comparative study of morphological change during myoregeneration in cardiotoxin-injured muscle

    CHAIYASING Rattanatrai, 割田克彦, 割田克彦, 割田克彦, 保坂善真, 保坂善真, 保坂善真

    日本解剖学会総会・全国学術集会講演プログラム・抄録集  2020 

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  • 筋組織修復過程におけるコンドロイチン硫酸合成・分解関連酵素の発現とその機能

    保坂善真, 保坂善真, 保坂善真, 鷲江壮太, CHAIYASHING Rattanatrai, 割田克彦, 割田克彦, 割田克彦

    日本糖質学会年会要旨集  2019 

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  • 筋再生過程でのコンドロイチン硫酸合成・分解酵素の動態

    鷲江壮太, 割田克彦, 保坂善真

    日本獣医学会学術集会講演要旨集  2019 

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    Language:Japanese  

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  • 末梢組織の局所で完結するα<sub>2</sub>アドレナリン受容体を介したTRPV1の抑制による鎮痛作用

    松下有美, 松下有美, 真鍋美樹, 樋口雅司, 保坂善真, 北村直樹, 澁谷泉

    日本獣医学会学術集会講演要旨集  2019 

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    Event date: 2019

    Language:Japanese  

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  • 上皮間葉転換(EMT)誘導前後におけるスタチンの制がん効果

    嶋倉弘晃, 割田克彦, 割田克彦, 石川拓郎, 保坂善真, 保坂善真

    日本獣医学会学術集会講演要旨集  2019 

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    Language:Japanese  

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  • ニホンジカ(Cervus nippon)鹿茸と消化管に含まれるグリコサミノグリカンの分析

    武田(奥田)尚子, 水本秀二, ZHANG Zui, KIM Soo-Ki, LEE Chi-Ho, JEON Byong-Tae, YEON Su-Jung, 保坂善真, 門松健治, 山田修平, 田村純一

    日本糖質学会年会要旨集  2019 

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  • スタチンが上皮系がん細胞の運動性に与える影響の検討

    石川拓郎, 石川拓郎, 割田克彦, 割田克彦, 保坂善真, 保坂善真

    日本獣医学会学術集会講演要旨集  2019 

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  • 透けてきたSkeleton構造-骨格筋・結合組織の形態形成機構とその利用- コンドロイチン硫酸を通して明らかとなった筋管の形成

    保坂 善真

    日本獣医学会学術集会講演要旨集  2018.8 

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  • 透けてきたSkeleton構造-骨格筋・結合組織の形態形成機構とその利用- コンドロイチン硫酸を通して明らかとなった筋管の形成

    保坂 善真

    日本獣医学会学術集会講演要旨集  2018.8  (公社)日本獣医学会

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  • 鶏脊髄accessory lobe内の神経様細胞には機能的な神経型電位依存性Na<sup>+</sup>チャネルが発現している

    松下有美, 北村直樹, 樋口雅司, 保坂善真, 澁谷泉

    日本獣医学会学術集会講演要旨集  2018 

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  • スタチン系薬剤とsiRNAの併用による相乗的制がん効果の検討

    石川拓郎, 石川拓郎, 割田克彦, 割田克彦, 保坂善真, 保坂善真

    日本獣医学会学術集会講演要旨集  2018  (公社)日本獣医学会

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  • コンドロイチン硫酸を通して明らかとなった筋管の形成

    保坂善真

    日本獣医学会学術集会講演要旨集  2018 

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  • アフリカツメガエル消化管のヒアルロニダーゼ発現解析

    伊藤慎治, 割田克彦, 保坂善真

    日本獣医学会学術集会講演要旨集  2018 

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  • 鳥取県秋里遺跡より出土した獣骨の動物種の同定

    森谷玲子, 高橋章司, 戸崎晃明, 戸崎晃明, 高須正規, 割田克彦, 保坂善真

    日本獣医学会学術集会講演要旨集  2017 

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  • スタチン系薬剤が癌の細胞死を引き起こす因子に関する研究

    石川拓郎, 割田克彦, 保坂善真

    日本獣医学会学術集会講演要旨集  2017  (公社)日本獣医学会

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  • アフリカツメガエル消化管のコンドロイチン硫酸の組成とコンドロイチン硫酸基転移酵素の発現解析

    岡秀俊, 武田(奥田)尚子, 田村純一, 割田克彦, 保坂善真

    日本獣医学会学術集会講演要旨集  2017 

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  • 伸長期におけるシカ角の組織学的特徴とコンドロイチン硫酸産生細胞の同定

    鈴木愛実, 武田(奥田)尚子, 田村純一, 割田克彦, 保坂善真

    日本獣医学会学術集会講演要旨集  2016  (公社)日本獣医学会

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  • 伸展刺激が骨芽細胞の分化に及ぼす影響

    青木亮, 中島純一, 北村直樹, 澁谷泉, 割田克彦, 保坂善真

    日本獣医学会学術集会講演要旨集  2016  (公社)日本獣医学会

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  • ラット視索上核および脳室周囲器官におけるTRPV1の探索

    大荒恵理, 中島純一, 北村直樹, 保坂善真, 澁谷泉

    日本獣医学会学術集会講演要旨集  2016  (公社)日本獣医学会

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  • ラット精細管におけるAMH,AMHR2発現と精子形成への関与

    太田正法, 田辺良子, 保坂善真, 山野好章, 大山建司

    日本内分泌学会雑誌  2016  (一社)日本内分泌学会

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  • デコリン糖鎖であるコンドロイチン硫酸Bによる筋分化の制御

    保坂善真, 三宅耶衣, 割田克彦

    日本糖質学会年会要旨集  2016 

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    Language:Japanese  

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  • アフリカツメガエル消化管のコンドロイチン硫酸組成とコンドロイチン硫酸基転移酵素の発現解析

    岡秀俊, 田村純一, 武田(奥田)尚子, 割田克彦, 保坂善真

    日本糖質学会年会要旨集  2016 

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  • Rab GTPase活性化因子TBC1D9のマウス精巣における発現と細胞内局在(Expression and intracellular localization of TBC1D9, a Rab GTPase-accelerating protein, in mouse testes)

    中村 有孝, 浅野 淳, 保坂 善真, 竹内 崇師, 岩永 敏彦, 山野 好章

    2015.10 

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    雄性生殖細胞内では、細胞の形態変化やアクロソーム形成の際に膜輸送が見られ、精子形成において重要である。TBCタンパク質ファミリーは、低分子量Gタンパク質Rabに対するGTPase活性化タンパク質として機能し、Rabタンパク質の機能を負に制御し、膜輸送を調節する因子として知られている。本研究において、我々はTBCタンパク質の一つであるTBC1D9のマウス組織における発現と、タンパク質の細胞内局在について検討した。その結果、Tbc1d9遺伝子は、成熟個体の精巣に強く発現することが明らかになった。さらに、Tbc1d9 mRNAは一次および二次精母細胞に発現しており、TBC1D9タンパク質は精母細胞と精子細胞で発現することがわかった。また、293T細胞にTBC1D9-GFP融合タンパク質を発現させると、融合タンパク質はエンドソームとゴルジ体に局在していた。特に、TBC1D9 isoform 1は恒常的活性型のRab7およびRab9変異体タンパク質が局在する細胞内部位に存在することが明らかになった。また、免疫沈降法を用いた解析により、TBC1D9-GFPタンパク質はRab7およびRab9と相互作用することがわかった。以上の結果より、TBC1D9タンパク質は主に精母細胞で発現し、Rab9およびRab7陽性の細胞内小胞に関連した膜輸送の調節に関わる可能性が示された。(著者抄録)

  • Expression and intracellular localization of TBC1D9, a Rab GTPase-accelerating protein, in mouse testes

    Yutaka Nakamura, Atsushi Asano, Yoshinao Hosaka, Takashi Takeuchi, Toshihiko Iwanaga, Yoshiaki Yamano

    Experimental Animals  2015.10  INT PRESS EDITING CENTRE INC

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    ©2015 Japanese Association for Laboratory Animal Science. Membrane trafficking in male germ cells contributes to their development via cell morphological changes and acrosome formation. TBC family proteins work as Rab GTPase accelerating proteins (GAPs), which negatively regulate Rab proteins, to mediate membrane trafficking. In this study, we analyzed the expression of a Rab GAP, TBC1D9, in mouse organs and the intracellular localization of the gene products. Tbc1d9 showed abundant expression in adult mice testis. We found that the Tbc1d9 mRNA was expressed in primary and secondary spermatocytes, and that the TBC1D9 protein was expressed in spermatocytes and round spermatids. In 293T cells, TBC1D9-GFP proteins were localized in the endosome and Golgi apparatus. Compartments that were positive for the constitutive active mutants of Rab7 and Rab9 were also positive for TBC1D9 isoform 1. In addition, TBC1D9 proteins were associated with Rab7 and Rab9, respectively. These results indicate that TBC1D9 is expressed mainly in spermatocytes, and suggest that TBC1D9 regulates membrane trafficking pathways related to Rab9- or Rab7-positive vesicles.

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  • 骨の形成者と破壊者を制御するコンドロイチン硫酸

    保坂善真, 田村純一

    日本農芸化学会大会講演要旨集(Web)  2015 

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  • 筋分化過程でのデコリンの機能と役割の解析

    三宅耶衣, 割田克彦, 西邑隆徳, 保坂善真

    日本獣医学会学術集会講演要旨集  2015  (公社)日本獣医学会

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  • 癌細胞のスタチン系薬剤感受性を決める因子に関する研究

    割田克彦, 割田克彦, 保坂善真, 三觜友子, 三木崇範, OLTVAI Zoltan

    日本獣医学会学術集会講演要旨集  2015  (公社)日本獣医学会

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  • マウス精巣におけるNkx2‐6の発現と転写調節機能の解析

    小山崇裕, 中村有孝, 保坂善真, 竹内崇師, 浅野淳, 山野好章

    日本獣医学会学術集会講演要旨集  2015  (公社)日本獣医学会

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  • フグ目魚類の著しく短縮した脊髄から見た脊髄終糸の形成

    上原正人, 保坂善真

    日本獣医学会学術集会講演要旨集  2015  (公社)日本獣医学会

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  • Fucosyl chondroitin sulfate from sea cucumber - chemical synthesis and structural analysis

    Jun-ichi Tamura, Haruna Tanaka, Ayumi Nakamura, Ryosuke Toita, Yoshinao Z. Hosaka, Ken-ichi Nagamine, Ayaka Himono, Naoko Takeda

    GLYCOBIOLOGY  2014.11 

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  • Fucosyl chondroitin sulfate from sea cucumber - chemical synthesis and structural analysis

    Jun-ichi Tamura, Haruna Tanaka, Ayumi Nakamura, Ryosuke Toita, Yoshinao Z. Hosaka, Ken-ichi Nagamine, Ayaka Himono, Naoko Takeda

    GLYCOBIOLOGY  2014.11  OXFORD UNIV PRESS INC

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  • 細胞形態ダイナミクスをリードする分子群を求めて コンドロイチン硫酸 耳かき1杯の糖が決定する骨の運命

    保坂 善真

    日本獣医学会学術集会講演要旨集  2014.8 

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  • 細胞形態ダイナミクスをリードする分子群を求めて コンドロイチン硫酸 耳かき1杯の糖が決定する骨の運命

    保坂 善真

    日本獣医学会学術集会講演要旨集  2014.8  (公社)日本獣医学会

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  • ラット視索上核ニューロンに発現するTRPV1の機能解析および浸透圧受容器発現細胞の解析

    守屋大樹, 柴崎梨奈, 萱野智彦, 竹渕奈美, 市村桃子, 北村直樹, 浅野淳, 保坂善真, DAYANITHI Govindan, 澁谷泉

    日本獣医学会学術集会講演要旨集  2014  (公社)日本獣医学会

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  • コンドロイチン硫酸―耳かき1杯の糖が決定する骨の運命

    保坂善真

    日本獣医学会学術集会講演要旨集  2014 

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  • キンコ(Cucumaria frondosa)由来糖鎖の脂肪細胞分化抑制活性に関する研究

    三好円香, 田村純一, 桧物綾香, 永峰賢一, 保坂善真

    日本獣医学会学術集会講演要旨集  2014  (公社)日本獣医学会

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  • ヒトコブラクダにおける網膜神経節細胞トポグラフィーと視力 上方向からの再現(Retinal Ganglion Cell Topography and Visual Acuity in Dromedary Camel: Re-Scene From Above)

    Abdo Mohamed, Shoghy Khaled, 保坂 善真, 上原 正人

    日本獣医学会学術集会講演要旨集  2013.8 

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  • ヒトコブラクダにおける網膜神経節細胞トポグラフィーと視力 上方向からの再現(Retinal Ganglion Cell Topography and Visual Acuity in Dromedary Camel: Re-Scene From Above)

    Abdo Mohamed, Shoghy Khaled, 保坂 善真, 上原 正人

    日本獣医学会学術集会講演要旨集  2013.8  (公社)日本獣医学会

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  • 成熟ラット精巣における新規スフィンゴシン1リン酸受容体モチーフ蛋白質遺伝子の発現(Expression of a Novel Sphingosine 1-Phosphate Receptor Motif Protein Gene in Maturing Rat Testes)

    Khalid Ahmed Magzoub, 浅野 淳, 保坂 善真, 山野 好章

    日本獣医学会学術集会講演要旨集  2013.3 

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  • 成熟ラット精巣における新規スフィンゴシン1リン酸受容体モチーフ蛋白質遺伝子の発現(Expression of a Novel Sphingosine 1-Phosphate Receptor Motif Protein Gene in Maturing Rat Testes)

    Khalid Ahmed Magzoub, 浅野 淳, 保坂 善真, 山野 好章

    日本獣医学会学術集会講演要旨集  2013.3  (公社)日本獣医学会

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  • 骨芽細胞活性化ペプチド(OBAP)産生細胞の同定とその分泌様態

    曽我部舞奈, 保坂善真, 山野好章, 上原正人

    日本獣医学会学術集会講演要旨集  2013  (公社)日本獣医学会

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  • 破骨細胞の分化と骨吸収能をコンドロイチン硫酸は抑制する

    近藤竜明, 保坂善真, 田村純一, 上原正人

    日本獣医学会学術集会講演要旨集  2013  (公社)日本獣医学会

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  • 発生早期の眼圧低下が眼球拡張と網膜形成に及ぼす影響

    保坂善真, 田中智子, 上原正人

    日本解剖学会総会・全国学術集会講演プログラム・抄録集  2013 

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  • 成熟期ラット精巣に発現するガン抑制因子TUSC3の解析

    KHALID Ahmed Magzoub, 浅野淳, 保坂善真, 竹内崇師, 大山建司, 山野好章

    日本分子生物学会年会プログラム・要旨集(Web)  2013 

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  • ニワトリ膵島におけるGLP‐1受容体局在に関する形態学的研究

    渡邉敬文, 保坂善真, 下里剛士, 西村佳, 竹本千尋, 平松浩二

    日本獣医学会学術集会講演要旨集  2013  (公社)日本獣医学会

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  • コンドロイチン硫酸‐Eの骨形成能:生体内での検証

    朝倉ひとみ, 保坂善真, 田村純一, 上原正人

    日本獣医学会学術集会講演要旨集  2013  (公社)日本獣医学会

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  • デコリンはインスリンと結合し細胞分化を調整している可能性がある

    保坂善真, 上原正人

    日本獣医学会学術集会講演要旨集  2013  (公社)日本獣医学会

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  • Rab調節因子Tbc1d9のマウス精巣での発現および細胞内局在の解析

    中村有孝, 保坂善真, 竹内崇師, 浅野淳, 山野好章

    日本獣医学会学術集会講演要旨集  2013  (公社)日本獣医学会

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  • ウシ血リンパ節におけるHEVマーカの異なった差次的発現(Differential expression of HEV marker in bovine hemal node)

    張 維東, 保田 昌宏, 保坂 善真, 那須 哲夫

    日本獣医学会学術集会講演要旨集  2012.8 

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  • ウシ血リンパ節におけるHEVマーカの異なった差次的発現(Differential expression of HEV marker in bovine hemal node)

    張 維東, 保田 昌宏, 保坂 善真, 那須 哲夫

    日本獣医学会学術集会講演要旨集  2012.8  (公社)日本獣医学会

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  • 獣医学領域における化学遺伝学の最前線 コンドロイチン硫酸の糖鎖構造が決定する細胞分化の方向性

    保坂 善真, 田村 純一, 一柳 剛, 上原 正人

    日本獣医学会学術集会講演要旨集  2012.3 

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  • ウシ血リンパ節における血液の供給と微小血管系に関する研究(Studies on the blood supply and microvasculature system in bovine hemal node)

    Zhang Weidong, 保田 昌宏, 保坂 善真, 那須 哲夫

    日本獣医学会学術集会講演要旨集  2012.3 

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  • 獣医学領域における化学遺伝学の最前線 コンドロイチン硫酸の糖鎖構造が決定する細胞分化の方向性

    保坂 善真, 田村 純一, 一柳 剛, 上原 正人

    日本獣医学会学術集会講演要旨集  2012.3  (公社)日本獣医学会

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  • ウシ血リンパ節における血液の供給と微小血管系に関する研究(Studies on the blood supply and microvasculature system in bovine hemal node)

    Zhang Weidong, 保田 昌宏, 保坂 善真, 那須 哲夫

    日本獣医学会学術集会講演要旨集  2012.3  (公社)日本獣医学会

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  • マウス精巣におけるRab調節因子Tbc1d9の発現解析

    中村有孝, 保坂善真, 竹内崇師, 浅野淳, 山野好章

    日本獣医学会学術集会講演要旨集  2012  (公社)日本獣医学会

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  • コンドロイチン硫酸の糖鎖構造が決定する細胞分化の方向性

    保坂善真, 田村純一, 一柳剛, 上原正人

    日本獣医学会学術集会講演要旨集  2012 

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  • イヌ輝板の形態について

    上原正人, 篠崎綾, 保坂善真

    日本獣医師会獣医学術学会年次大会講演要旨集  2012 

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  • VASOPRESSIN-INDUCED [CA(2+)]I INCREASE IN NON-NEURONAL CELLS FROM RAT DORSAL ROOT GANGLIA

    G. Dayanithi, T. Moriya, T. Kayano, N. Kitamura, Y. Hosaka, C. Viero, E. C. Toescu, O. Forostyak, Shibuya, I

    GLIA  2011.10 

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  • VASOPRESSIN-INDUCED [CA(2+)]I INCREASE IN NON-NEURONAL CELLS FROM RAT DORSAL ROOT GANGLIA

    G. Dayanithi, T. Moriya, T. Kayano, N. Kitamura, Y. Hosaka, C. Viero, E. C. Toescu, O. Forostyak, Shibuya, I

    GLIA  2011.10  WILEY-BLACKWELL

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  • 獣医学領域における化学遺伝学の黎明 糖鎖を修飾する硫酸基の機能~コンドロイチン硫酸種の脂肪分化への作用

    保坂 善真, 一柳 剛, 田村 純一, 上原 正人

    日本獣医学会学術集会講演要旨集  2011.3 

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  • 獣医学領域における化学遺伝学の黎明 糖鎖を修飾する硫酸基の機能〜コンドロイチン硫酸種の脂肪分化への作用

    保坂 善真, 一柳 剛, 田村 純一, 上原 正人

    日本獣医学会学術集会講演要旨集  2011.3  (公社)日本獣医学会

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  • 糖鎖を修飾する硫酸基の機能~コンドロイチン硫酸種の脂肪分化への作用

    保坂善真, 一柳剛, 田村純一, 上原正人

    日本獣医学会学術集会講演要旨集  2011 

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  • ナマコ由来糖鎖の脂肪分化に関する研究

    保坂善真, 田村純一, 上原正人

    解剖学雑誌  2011 

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  • コンドロイチン硫酸Eの骨形成能

    岩井勇次, 保坂善真, 田村純一, 上原正人

    日本獣医学会学術集会講演要旨集  2011  (公社)日本獣医学会

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  • ウシ血リンパにおける免疫担当細胞の局在とポピュレーション解析

    張維東, 張維東, 保田昌宏, 高取瞳, 保坂善真, 那須哲夫

    日本獣医学会学術集会講演要旨集  2011  (公社)日本獣医学会

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  • ラット精巣におけるガン・精巣(CT)抗原遺伝子の発現解析(Expression analysis of CT (cancer/testis) antigen gene in maturing rat testis)

    山野 好章, 浅野 淳, Khalid Ahmed Magzoub, 保坂 善真, 太田 正法, 大山 建司

    日本生化学会大会・日本分子生物学会年会合同大会講演要旨集  2010.12 

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  • ラット精巣におけるガン・精巣(CT)抗原遺伝子の発現解析(Expression analysis of CT (cancer/testis) antigen gene in maturing rat testis)

    山野 好章, 浅野 淳, Khalid Ahmed Magzoub, 保坂 善真, 太田 正法, 大山 建司

    日本生化学会大会・日本分子生物学会年会合同大会講演要旨集  2010.12  (公社)日本生化学会

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  • マウス網膜と視神経の形成におけるコンドロイチン硫酸プロテオグリカンの時空間的分布(Spatiotemporal distribution of chondroitin sulfate proteoglycans in the developing mouse retina and optic nerve)

    Ali Safwat, Ali Mohamed, 保坂 善真, 上原 正人

    日本獣医学会学術集会講演要旨集  2010.9 

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  • マウス網膜と視神経の形成におけるコンドロイチン硫酸プロテオグリカンの時空間的分布(Spatiotemporal distribution of chondroitin sulfate proteoglycans in the developing mouse retina and optic nerve)

    Ali Safwat, Ali Mohamed, 保坂 善真, 上原 正人

    日本獣医学会学術集会講演要旨集  2010.9  (公社)日本獣医学会

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  • Comparative study of the characteristics and properties of tendinocytes derived from three tendons in the equine forelimb (vol 42, pg 9, 2010)

    Yoshinao Z. Hosaka, Hideyuki Takahashi, Takehiro Uratsuji, Parasarn Tangkawattana, Hiromi Ueda, Kazushige Takehana

    TISSUE & CELL  2010.8 

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  • Comparative study of the characteristics and properties of tendinocytes derived from three tendons in the equine forelimb (vol 42, pg 9, 2010)

    Yoshinao Z. Hosaka, Hideyuki Takahashi, Takehiro Uratsuji, Parasarn Tangkawattana, Hiromi Ueda, Kazushige Takehana

    TISSUE & CELL  2010.8  CHURCHILL LIVINGSTONE

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  • 鮭皮コラーゲンペプチドの牛蹄冠部周囲真皮への影響と効果

    保坂 善真, 後藤 千尋, 阿部 敬, 永易 彩, 植田 弘美, 竹花 一成

    日本獣医師会雑誌 = Journal of the Japan Veterinary Medical Association  2010.1 

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    牛に飼料とともに鮭皮由来コラーゲンペプチド(MCP)を8週間摂取させ、前肢蹄冠の背側、外側、指間の真皮を解析した。MCP群の背側と外側の蹄冠皮膚は真皮の厚さ、線維芽細胞数、コラーゲン細線維の直径が増加、上昇した。MCP群の指間真皮の線維芽細胞数は増加したが、厚さは低下し、コラーゲン細線維直径は減少した。線維径を調整するデルマタン硫酸とV型コラーゲンはMCP群のすべての部位で増加し、特に指間での増加が顕著であった。結合組織の強さの指数MADは背側と外側で増加したが指間では減少した。MCP投与による指間真皮のユニークな応答は細いコラーゲン細線維を集めて柔軟な結合組織を形成し、着地時の衝撃を緩衝するためと考察した。MCPは線維芽細胞に作用し蹄冠の各部位に即した太さのコラーゲン細線維を産生させ、真皮構造を強化する効果があると考えられた。

  • 鮭皮コラーゲンペプチドの牛蹄冠部周囲真皮への影響と効果

    保坂 善真, 後藤 千尋, 阿部 敬, 永易 彩, 植田 弘美, 竹花 一成

    日本獣医師会雑誌 = Journal of the Japan Veterinary Medical Association  2010.1  日本獸医師会

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    牛に飼料とともに鮭皮由来コラーゲンペプチド(MCP)を8週間摂取させ、前肢蹄冠の背側、外側、指間の真皮を解析した。MCP群の背側と外側の蹄冠皮膚は真皮の厚さ、線維芽細胞数、コラーゲン細線維の直径が増加、上昇した。MCP群の指間真皮の線維芽細胞数は増加したが、厚さは低下し、コラーゲン細線維直径は減少した。線維径を調整するデルマタン硫酸とV型コラーゲンはMCP群のすべての部位で増加し、特に指間での増加が顕著であった。結合組織の強さの指数MADは背側と外側で増加したが指間では減少した。MCP投与による指間真皮のユニークな応答は細いコラーゲン細線維を集めて柔軟な結合組織を形成し、着地時の衝撃を緩衝するためと考察した。MCPは線維芽細胞に作用し蹄冠の各部位に即した太さのコラーゲン細線維を産生させ、真皮構造を強化する効果があると考えられた。

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  • 間葉系幹細胞の骨分化過程にデコリンは必要か―生体内での検証―

    保坂善真, 柄武志, 今川智敬, 上原正人

    解剖学雑誌  2010  (一社)日本解剖学会

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  • 羊眼球における線維性輝板の分布および網膜色素上皮層との関係

    篠崎綾, 保坂善真, 今川智敬, 上原正人

    解剖学雑誌  2010  (一社)日本解剖学会

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  • マナマコ(Stichopus japonicus)より抽出した糖鎖の脂肪分化抑制活性

    保坂善真, 田村純一, 上原正人

    日本結合組織学会学術大会抄録集  2010 

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  • The role of a neuromuscular junctions-related factor, agrin, for murine implantation.

    TERAKAWA Jumpei, WAKITANI Shoichi, SUGIYAMA Makoto, INOUE Naoko, OHMORI Yasushige, KISO Yasuo, HOSAKA Yoshinao, HONDO Eiichi

    The Journal of Reproduction and Development Supplement  2010  THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

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  • ニワトリ胚眼球におけるデコリンの分布と眼球サイズに与える影響

    岩佐歩, 保坂善真, 上原正人

    日本獣医学会学術集会講演要旨集  2010  (公社)日本獣医学会

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  • コンドロイチン硫酸Eの脂肪細胞分化抑制作用

    柘植亜衣子, 保坂善真, 田村純一, 上原正人

    日本獣医学会学術集会講演要旨集  2010  (公社)日本獣医学会

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  • ナマコ由来糖鎖の脂肪細胞の分化抑制活性

    保坂善真, 田村純一, 上原正人

    日本獣医学会学術集会講演要旨集  2010  (公社)日本獣医学会

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  • ウマ眼球におけるプロテオグリカンの発現と分布

    福原匡志, 高木智史, 保坂善真, 上原正人

    日本獣医学会学術集会講演要旨集  2010  (公社)日本獣医学会

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  • Efficacy of Antivascular Photodynamic Therapy Using Benzoporphyrin Derivative Monoacid Ring A (BPD-MA) in 14 Dogs with Oral and Nasal Tumors

    Tomohiro Osaki, Satoshi Takagi, Yuki Hoshino, Masahiro Okumura, Tsuyoshi Kadosawa, Toru Fujinaga

    JOURNAL OF VETERINARY MEDICAL SCIENCE  2009.2 

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  • Efficacy of Antivascular Photodynamic Therapy Using Benzoporphyrin Derivative Monoacid Ring A (BPD-MA) in 14 Dogs with Oral and Nasal Tumors

    Tomohiro Osaki, Satoshi Takagi, Yuki Hoshino, Masahiro Okumura, Tsuyoshi Kadosawa, Toru Fujinaga

    JOURNAL OF VETERINARY MEDICAL SCIENCE  2009.2  JAPAN SOC VET SCI

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    Antivascular photodynamic therapy (PDT) Suppresses tumor growth and prolonged the survival in solid tumor-bearing mice. The purpose of this study was to assess the efficacy of antivascular PDT using BPD-MA for treatment of oral and nasal tumors in 14 dogs. At 15 min after initiating intravenous infusion of 0.5 mg/kg benzoporphyrin derivative monoacid ring A, tumors were irradiated with laser light at 690 nm emitted by a diode laser, The 1-year survival rate of 7 dogs with oral tumors was 71%. The 1-year Survival rate of 7 does with nasal tumors was 57%. Imaging of each tumor was performed by using angiographic computed tomography before and after each antivascular PDT. Contrast-enhanced turners were observed before antivascular PDT, but these tumors were not enhanced with contrast medium following antivascular PDT. Antivascular PDT is suggested to be a promising method for dogs with oral and nasal tumors that cannot be effectively treated with current antitumor therapies.

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  • 間葉系幹細胞の骨分化過程におけるデコリンの役割―生体内での検証―

    保坂善真, 柄武志, 今川智敬, 上原正人

    日本獣医学会学術集会講演要旨集  2009  (公社)日本獣医学会

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  • 間葉系幹細胞から骨芽細胞への分化過程でのデコリンの関与と役割

    保坂善真, 小海康夫, 今川智敬, 上原正人

    解剖学雑誌  2009  (一社)日本解剖学会

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  • 羊の網膜における視神経細胞と視細胞の分布およびこれらと輝板の関係

    篠崎綾, 保坂善真, 今川智敬, 上原正人

    解剖学雑誌  2009  (一社)日本解剖学会

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  • 甲状腺ホルモン不足が小脳形態に及ぼす影響

    根木慶彦, 長谷部美紀, 保坂善真, 上原正人

    日本獣医学会学術集会講演要旨集  2009  (公社)日本獣医学会

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  • ラット精巣における結腸ガン抗原遺伝子の発現解析

    神尾高志, 浅野淳, 保坂善真, 大山建司, 山野好章

    日本獣医学会学術集会講演要旨集  2009  (公社)日本獣医学会

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  • ラットドライアイモデルにおける角膜表面ムチンの分布

    中嶋英雄, 今田敏博, 増田望, 中村滋, 保坂善真, 上原正人, 坪田一男

    角膜カンファランス・日本角膜移植学会プログラム・抄録集  2009 

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  • ヒツジ眼球における視神経細胞,視細胞および輝板の分布と相互関係

    篠崎綾, 保坂善真, 今川智敬, 上原正人

    日本獣医学会学術集会講演要旨集  2009  (公社)日本獣医学会

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  • ウマの線維性輝板の超微形態学的観察

    高木智史, 篠崎綾, 保坂善真, 今川智敬, 上原正人

    日本獣医学会学術集会講演要旨集  2009  (公社)日本獣医学会

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  • Decorin regulate osteoblastic differentiation of mesenchymal stem cell.

    Hosaka Y, Tsuka T, Imagawa T, Uehara M

    Proc. YSF2009.  2009 

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  • 筋芽細胞による自律的な筋線維型制御における機械的伸展刺激の影響

    中田 后紀, 川崎 佑華, 大山 竜輝, 前野 岳大, 今給黎 燿, 小川 陽平, 大崎 莉果, 小川 蒼生, 保坂 善真, 中村 真子, 辰巳 隆一, 鈴木 貴弘

    日本畜産学会大会講演要旨集  2023.9  (公社)日本畜産学会

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  • 物理刺激培養モデルを用いた筋管の筋線維型制御機構の変化

    鈴木 貴弘, 川崎 佑華, 中田 后紀, 大山 竜輝, 江草 愛, 保坂 善真, 前野 岳大, 今給黎 燿, 小川 陽平, 大崎 莉果, 小川 蒼生, 中村 真子, 辰巳 隆一

    日本栄養・食糧学会大会講演要旨集  2024.4  (公社)日本栄養・食糧学会

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  • 中枢NPYはニワトリヒナの脾臓における暑熱ストレス応答を軽減し抗酸化機能を強化する(Central NPY attenuates splenic heat stress and enhances an antioxidative function in chicks)

    Nishimura Haruka, Wang Ying, Elhussiny Mohamed Z., Nishimura Shotaro, Hosaka Yoshinao, Takahashi Eiki, Chowdhury Vishwajit S.

    Experimental Animals  2024  (公社)日本実験動物学会

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  • 低エストロゲン状態および高脂肪食誘発肥満マウスの脂肪組織の特性(Adipose Tissue Properties in the Low Estrogen State and High Fat Diet-Induced Obesity Mice)

    Danang Dwi Cahyadi , 割田 克彦, 保坂 善真

    日本獣医学会学術集会講演要旨集  2022.9  (公社)日本獣医学会

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  • ニワトリの脊髄副葉には神経細胞型電位依存性ナトリウムチャネルを発現する神経様細胞が存在する(Neuron-like cells expressing the neuronal-type voltage gated sodium channels are present in the chick spinal accessory lobes)

    Matsushita Yumi, Kitamura Naoki, Higuchi Masashi, Hosaka Yoshinao, Shibuya Izumi

    The Journal of Physiological Sciences  2022.12  (一社)日本生理学会

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  • コレステロール側鎖切断酵素の発現抑制が前立腺がん細胞の上皮間葉転換およびスタチン系薬剤感受性に及ぼす影響

    田代 二朗, 杉浦 曜大, 石川 拓郎, 保坂 善真, 割田 克彦

    日本獣医学会学術集会講演要旨集  2022.9  (公社)日本獣医学会

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  • 3T3-L1脂肪形成におけるコンドロイチン/デルマタン硫酸プロファイルの変化(Changes in chondroitin/dermatan sulfate profile during 3T3-L1 adipogenesis)

    Danang Dwi Cahyadi , 割田 克彦, 武田 尚子[奥田], 田村 純一, 保坂 善真

    日本獣医学会学術集会講演要旨集  2023.9  (公社)日本獣医学会

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  • 2023年度 旗影会研究助成報告書

    保坂 善真

    一般財団法人 旗影会   2024.5

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  • 透けてきたSkeleton構造-骨格筋・結合組織の形態形成機構とその利用- コンドロイチン硫酸を通して明らかとなった筋管の形成

    保坂 善真

    日本獣医学会学術集会講演要旨集   2018.8

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  • Rab GTPase活性化因子TBC1D9のマウス精巣における発現と細胞内局在(Expression and intracellular localization of TBC1D9, a Rab GTPase-accelerating protein, in mouse testes)

    中村 有孝, 浅野 淳, 保坂 善真, 竹内 崇師, 岩永 敏彦, 山野 好章

    2015.10

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    雄性生殖細胞内では、細胞の形態変化やアクロソーム形成の際に膜輸送が見られ、精子形成において重要である。TBCタンパク質ファミリーは、低分子量Gタンパク質Rabに対するGTPase活性化タンパク質として機能し、Rabタンパク質の機能を負に制御し、膜輸送を調節する因子として知られている。本研究において、我々はTBCタンパク質の一つであるTBC1D9のマウス組織における発現と、タンパク質の細胞内局在について検討した。その結果、Tbc1d9遺伝子は、成熟個体の精巣に強く発現することが明らかになった。さらに、Tbc1d9 mRNAは一次および二次精母細胞に発現しており、TBC1D9タンパク質は精母細胞と精子細胞で発現することがわかった。また、293T細胞にTBC1D9-GFP融合タンパク質を発現させると、融合タンパク質はエンドソームとゴルジ体に局在していた。特に、TBC1D9 isoform 1は恒常的活性型のRab7およびRab9変異体タンパク質が局在する細胞内部位に存在することが明らかになった。また、免疫沈降法を用いた解析により、TBC1D9-GFPタンパク質はRab7およびRab9と相互作用することがわかった。以上の結果より、TBC1D9タンパク質は主に精母細胞で発現し、Rab9およびRab7陽性の細胞内小胞に関連した膜輸送の調節に関わる可能性が示された。(著者抄録)

    DOI: 10.1538/expanim.15-0016

  • 細胞形態ダイナミクスをリードする分子群を求めて コンドロイチン硫酸 耳かき1杯の糖が決定する骨の運命

    保坂 善真

    日本獣医学会学術集会講演要旨集   2014.8

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  • ヒトコブラクダにおける網膜神経節細胞トポグラフィーと視力 上方向からの再現(Retinal Ganglion Cell Topography and Visual Acuity in Dromedary Camel: Re-Scene From Above)

    Abdo Mohamed, Shoghy Khaled, 保坂 善真, 上原 正人

    日本獣医学会学術集会講演要旨集   2013.8

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  • 成熟ラット精巣における新規スフィンゴシン1リン酸受容体モチーフ蛋白質遺伝子の発現(Expression of a Novel Sphingosine 1-Phosphate Receptor Motif Protein Gene in Maturing Rat Testes)

    Khalid Ahmed Magzoub, 浅野 淳, 保坂 善真, 山野 好章

    日本獣医学会学術集会講演要旨集   2013.3

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  • ウシ血リンパ節におけるHEVマーカの異なった差次的発現(Differential expression of HEV marker in bovine hemal node)

    張 維東, 保田 昌宏, 保坂 善真, 那須 哲夫

    日本獣医学会学術集会講演要旨集   2012.8

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  • ウシ血リンパ節における血液の供給と微小血管系に関する研究(Studies on the blood supply and microvasculature system in bovine hemal node)

    Zhang Weidong, 保田 昌宏, 保坂 善真, 那須 哲夫

    日本獣医学会学術集会講演要旨集   2012.3

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  • 獣医学領域における化学遺伝学の最前線 コンドロイチン硫酸の糖鎖構造が決定する細胞分化の方向性

    保坂 善真, 田村 純一, 一柳 剛, 上原 正人

    日本獣医学会学術集会講演要旨集   2012.3

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  • 獣医学領域における化学遺伝学の黎明 糖鎖を修飾する硫酸基の機能~コンドロイチン硫酸種の脂肪分化への作用

    保坂 善真, 一柳 剛, 田村 純一, 上原 正人

    日本獣医学会学術集会講演要旨集   2011.3

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  • ラット精巣におけるガン・精巣(CT)抗原遺伝子の発現解析(Expression analysis of CT (cancer/testis) antigen gene in maturing rat testis)

    山野 好章, 浅野 淳, Khalid Ahmed Magzoub, 保坂 善真, 太田 正法, 大山 建司

    日本生化学会大会・日本分子生物学会年会合同大会講演要旨集   2010.12

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  • マウス網膜と視神経の形成におけるコンドロイチン硫酸プロテオグリカンの時空間的分布(Spatiotemporal distribution of chondroitin sulfate proteoglycans in the developing mouse retina and optic nerve)

    Ali Safwat, Ali Mohamed, 保坂 善真, 上原 正人

    日本獣医学会学術集会講演要旨集   2010.9

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  • Comparative study of the characteristics and properties of tendinocytes derived from three tendons in the equine forelimb (vol 42, pg 9, 2010)

    Yoshinao Z. Hosaka, Hideyuki Takahashi, Takehiro Uratsuji, Parasarn Tangkawattana, Hiromi Ueda, Kazushige Takehana

    TISSUE & CELL   2010.8

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    DOI: 10.1016/j.tice.2010.08.001

  • 内毒素血症に関与したウマの疝痛における腸間膜動脈の形態学的研究

    日高 修平, 植田 弘美, 保坂 善真

    酪農学園大学紀要. 自然科学編 = Journal of the College of Dairying. Natural science   2008.10

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    ウマにおいて疝痛を伴った内毒素血症はしばしば発症する疾病であって、毒素によって腸間膜動脈には様々な程度の虚血、動脈の痙攣や壊死が引き起こされる。重篤な場合では、それらの臨床症状が疝痛の前駆的発症要因になることが報告されている。エンドトキシンが関与し疝痛を患ったウマでは、腸間膜の血管における障害はこれまでのところ大いに注目されてきている。そこで、この研究では内毒素血症を自然発症したサラブレットにおいて、腸間膜動脈の障害を形態学的に検索した。この研究で得られた結果は、腸間膜にみられる血管の組織構造に対するエンドトキシンの効果をより良く理解できるものと思われる。試料は3.0%グルタルアルデヒド溶液に固定後、Quetol 812に包埋した。包埋した電顕試料より厚切り切片および超薄切片を作製し、前者には1%トルイジンブルー染色を施した後、光学顕微鏡を用いて、後者には酢酸ウラニウムとクエン酸鉛による二重染色を行った後、透過型電子顕微鏡にて詳細に検索した。発症群では動脈壁において血管内皮細胞の剥離、出血像、歪な形を呈した中膜平滑筋細胞や融解壊死した顆粒球などの血液細胞、空胞などが多数観察された。これらの結果は、すでに報告されているウマの内毒素血症の組織像と類似しており、また、エンドトキシンにより腸間膜中の動脈が損傷を受け、ウマの腸管において臓器性Shwartzman反応が起こったものと示唆された。

  • 角膜実質の再生医療 ブタ角膜実質細胞の培養法の検討

    永易 彩, 角出 泰造, 保坂 善真, 植田 弘美, 竹花 一成

    日本獣医学会学術集会講演要旨集   2008.3

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  • ノックダウン細胞を用いた、デコリンとコラーゲン細線維の相互関連の解析

    保坂 善真, 岩崎 俊輔, 植田 弘美, 小海 康夫, 竹花 一成

    解剖学雑誌   2007.6

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  • ウマ浅指屈筋腱炎内の肉芽組織におけるMMP-13 mRNA発現

    野村 正幸, 保坂 善真, 笠嶋 快周, 植田 弘美, 竹花 一成, 桑野 睦敏, 新井 克彦

    2007.6

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    ウマ浅指屈筋腱炎における細胞外マトリックス遺伝子発現の特徴を把握するため,DNAマイクロアレイ解析を実施したところ,屈腱炎組織においてMMP-13 mRNAの発現上昇が見られた.In situハイブリダイゼーションにより,屈腱炎内で増生した肉芽組織中の組維芽細胞様の細胞に強い陽性シグナルが確認されたが,健常腱組織では,その陽性細胞は腱内膜内にわずかに見られたのみであった.このことから,屈腱炎におけるMMP-13の重要な役割が示唆された.

  • ウマ浅指屈筋腱炎内の肉芽組織におけるMMP-13 mRNA発現(短報)(Active Expression of Matrix Metalloproteinase-13 mRNA in the Granulation Tissue of Equine Superficial Digital Flexor Tendinitis)

    野村 正幸, 保坂 善真, 笠嶋 快周, 植田 弘美, 竹花 一成, 桑野 睦敏, 新井 克彦

    2007.6

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    DOI: 10.1292/jvms.69.637

  • 皮膚真皮のコラーゲン線維とグリコサミノグリカンに対するコラーゲンペプチド摂取の効果

    松田 直也, 小山 洋一, 保坂 善真, 植田 弘美, 渡辺 敬文, 新谷 隆行, 入江 伸吉, 竹花 一成

    ビタミン   2006.8

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  • 第31回家畜診療等技術全国研究集会入賞論文 乳牛の胎盤停滞と子宮小丘の形態の関連性

    三好 正一, 保坂 善真, 竹花 一成

    家畜診療   2006.1

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  • 腱の状態によるTNFαとTNF-R1を介する細胞内シグナル伝達因子の分布と発現の差違

    保坂 善真, 桐澤 力雄, 植田 弘美, ほか

    2005.10

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  • 腱の状態によるTNFαとTNF-R1を介する細胞内シグナル伝達因子の分布と発現の差違

    保坂 善真, 桐澤 力雄, 植田 弘美, 山口 守, 竹花 一成

    2005.10

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    生存とアポトーシスという全く相反する作用を細胞に誘導する炎症性サイトカインであるTNFαとTNFレセプター1(TNF-R1)を介する細胞内シグナル伝達関連因子(TRAF2, caspase-3)に着目し, 腱の状態(正常, 炎症および瘢痕形成腱)によるこれらの因子の分布と発現の差違を形態学的, 生化学的に明らかにした.ウマの浅指屈筋腱から正常, 炎症および瘢痕形成腱を採取し, 免疫染色とイムノブロット法で解析した.TNFαに関してはさらにin situ hybridization法およびRT-PCR-サザンブロット法でmRNAの発現を検索した.TNFαはいずれの状態でも発現の程度に違いはあったがmRNAの発現を認めた.TNF-R1, TRAF2およびcaspase-3は炎症腱で強い反応を示した.また, TRAF2は正常腱でも比較的多量の存在を認めた.正常および瘢痕形成腱ではこれらの因子は微弱であった.活性型caspase-3は炎症腱でのみ検出できた.炎症時にこれらのTNF-R1を介した細胞内シグナル伝達因子の産生が増強されたことより, 腱細胞にもTNFαによるTNF-R1を介した生存とアポトーシスの2種類のシグナル伝達経路が存在していると推察した.

    DOI: 10.1292/jvms.67.985

  • デコリンによるウマ浅指屈筋腱のコラーゲン細線維直径の調節(Control of the Collagen Fibril Diameter in the Equine Superficial Digital Flexor Tendon in Horses by Decorin)

    渡辺 敬文, 保坂 善真, 山本 悦子, 植田 弘美, 菅原 健治, 高橋 平七郎, 竹花 一成

    2005.9

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  • デコリンによるウマ浅指屈筋腱のコラーゲン細線維直径の調節

    渡辺 敬文, 保坂 善真, 山本 悦子, 植田 弘美, 菅原 健治, 高橋 平七郎, 竹花 一成

    2005.9

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    ウマ浅指屈筋腱のコラーゲン細線維の直径分布様式は周知の様に中央部と遠位部により異なる.本研究ではウマ浅指屈筋腱におけるコラーゲン細線維の部位差を起こす要因を透過型電子顕微鏡と超高圧電子顕微鏡による観察と生化学的解析を用いて明らかにした.ウマ浅指屈筋腱は中手骨近位部から中手骨遠位部にかけて太いコラーゲン細線維の分布は増加するが, コラーゲン細線維の密度は減少していた.中手骨中央部では歪な横断像を呈する太いコラーゲン細線維が他の部位より多く認められた.この歪な形のコラーゲン細線維の3次元立体構築像によりこれらの線維は細いコラーゲン細線維が太い線維に融合することにより形成されることが明らかになった.コラーゲン細線維の側面の融合を抑制すると報告されているデコリンの量は中手骨遠位部に向かうにつれて減少していた.一方, デコリンの分子量は中手骨遠位部に向かうにつれ徐々に大きくなっていた.これらの結果はウマ浅指屈筋腱におけるコラーゲン細線維の部位差が, 少なくともコラーゲン細線維の集合とデコリンの量と大きさにより影響されていることを示すものであった.

    DOI: 10.1292/jvms.67.855

  • 若齢期のウマにおける伸筋腱および屈筋腱の組織学的変化

    山崎 維嗣, 渡辺 敬文, 保坂 善真, 植田 弘美, 竹花 一成

    日本獣医学会学術集会講演要旨集   2005.8

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  • ウマ浅指屈筋腱のコラーゲン細線維直径を変化させる分子要因について

    渡辺 敬文, 今村 保忠, 保坂 善真, 植田 弘美, 竹花 一成

    日本獣医学会学術集会講演要旨集   2005.8

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  • 犬の腫瘍におけるマトリクスメタロプロテイナーゼインヒビターRECKの発現(外科学)

    高木 哲, 加藤 友香, 浅野 和之, 大崎 智弘, ボスナコフスキー ダルコ, 星野 有希, 奥村 正裕, 廉澤 剛, 藤永 徹

    2005.8

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    マトリクスメタロプロテイナーゼ(MMP), 特にMMP-2とMMP-9は腫瘍の浸潤, 転移に深く関わっているとされている.近年, これらのMMP活性を抑制する膜結合型タンパクであるRECKが発見され, ヒトの臨床例においてRECK発現量と腫瘍の悪性度には負の相関が認められている.本研究では, 犬の自然発生腫瘍症例におけるRECKmRNA発現量をリアルタイムPCR法にて定量し, 臨床的指標, MMP-9mRNAの相関について評価した.腫瘍組織とその由来する正常組織とのRECK発現量を比較したところ, いくつかの腫瘍組織で有意に発現が低くなっていた(p<0.05).年齢, 性別, リンパ節および肺転移の有無との有意差はそれぞれ認められなかった.MMP-9とRECKには有意な相関関係が認められた.腫瘍症例の予後とRECK発現量の相関関係を明らかにするにはより詳細な検討が必要であると思われた.

    DOI: 10.1292/jvms.67.761

  • 腱変性過程での熱,炎症性サイトカインおよびゼラチナーゼの関連

    保坂 善真, 尾添 紗知子, 桐澤 力雄, 植田 弘美, 竹花 一成

    日本獣医学会学術集会講演要旨集   2005.8

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  • 犬の膜結合型内因性マトリクスメタロプロテイナーゼインヒビターRECKのクローニング(Molecular Cloning of Canine Membrane-Anchored Inhibitor of Matrix Metalloproteinase,RECK)

    高木 哲, 北村 剛規, 保坂 善真, 大崎 智弘, ダルコ・ボスナコフスキー, 廣澤 剛, 奥村 正裕, 藤永 徹

    2005.4

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  • 犬の膜結合型内因性マトリクスメタロプロテイナーゼインヒビターRECKのクローニング

    高木 哲, 北村 剛規, 保坂 善真, 大崎 智弘, ボスナコフスキー ダルコ, 廉澤 剛, 奥村 正裕, 藤永 徹

    2005.4

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    RECKは近年発見された膜結合型内因性マトリクスメタロプロテイナーゼインヒビターであり, ヒトの臨床例においてその発現量と腫瘍の悪性度に負の相関が認められることが報告されている.本研究では犬のRECK遺伝子のクローニングを行った.その結果, cDNAは2,913塩基で, ヒトと95.5&#37;, マウスと91.9&#37;の相同性を示す971残基のアミノ酸から構成されることがわかった.様々な正常組織でのmRNAの発現量をリアルタイムPCR法にて定量した結果, 肺と精巣で高い発現が認められたが, 腫瘍細胞では極めて低い発現のみ認められた.免疫染色では精子, 平滑筋, 偽重層上皮などに強い染色性が認められ, 発現ペクターを用いた実験では腫瘍の浸潤能が抑制されていた.

    DOI: 10.1292/jvms.67.385

  • 馬の腱の損傷程度を反映する血清中I型コラーゲン架橋N-末端テロペプチド濃度

    保坂 善真, 樋口 豪紀, 翁長 武紀, ほか

    日本獣医師会雑誌 = Journal of the Japan Veterinary Medical Association   2005.3

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    骨の代謝マーカーの一つであるI型コラーゲン架橋N-末端テロペプチド濃度(Nx)が馬の腱損傷後の治癒過程をモニタリングするマーカーとして有用であるかを検討した。血清中Nx濃度は腱損傷のない正常群と比較して、腱炎の急性期および亜急性期群で有意に高い値を示した。しかし、慢性期群と正常群間とのNx値には有意差が認められなかった。腱炎による腱のI型コラーゲンの分解は亜急性期までで、慢性期以降では腱組織の再構築が開始されることから、血清中Nx濃度は腱炎によるコラーゲン分解を鋭敏に反映していると考えられた。

  • ウマ浅指屈筋腱のコラーゲン細線維の形態は部位によって変化する

    渡辺 敬文, 保坂 善真, 植田 弘美, 竹花 一成

    日本獣医学会学術集会講演要旨集   2005.3

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  • 膝関節の靱帯の構造特性

    竹花 一成, 大友 由佳子, 松川 拓也, 渡辺 敬文, 保坂 善真, 植田 弘美

    日本獣医学会学術集会講演要旨集   2005.3

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  • 馬の腱の損傷程度を反映する血清中I型コラーゲン架橋N-末端テロペプチド濃度

    保坂 善真, 樋口 豪紀, 翁長 武紀, 永田 廣, 上村 智希, 三角 一浩, 植田 弘美, 竹花 一成

    日本獣医師会雑誌 = Journal of the Japan Veterinary Medical Association   2005.3

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    骨の代謝マーカーの一つである1型コラーゲン架橋N-末端テロペプチド濃度 (NTx) が馬の腱損傷後の治癒過程をモニタリングするマーカーとして有用であるかを検討した.血清中NTx濃度は腱損傷のない正常群と比較して, 腱炎の急性期および亜急性期群で有意に高い値を示した.しかし, 慢性期群と正常群間とのNTx値には有意差が認められなかった.腱炎による腱のI型コラーゲンの分解は亜急性期までで, 慢性期以降では腱組織の再構築が開始されることから, 血清中NTx濃度は腱炎によるコラーゲン分解を鋭敏に反映していると考えられた.

    DOI: 10.12935/jvma1951.58.175

  • 乳牛の胎盤停滞と子宮小丘の形態の関連性

    三好 正一, 保坂 善真, 竹花 一成, 澤向 豊

    家畜診療   2005.2

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  • 腱細胞のデコリン及びTGF-β1の産生はデコリン遺伝子の発現抑制によって制御される

    保坂 善真, 桐澤 力雄, 真船 直樹, 渡辺 敬文, 尾添 紗知子, 植田 弘美, 竹花 一成

    2004.6

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  • 腱細胞へのデコリンアンチセンスオリゴヌクレオチド遺伝子の導入効果

    保坂 善真, 桐澤 力雄, 真船 直樹, 山本 悦子, 渡辺 敬文, 尾添 紗知子, 植田 弘美, 竹花 一成

    日本獣医学会学術集会講演要旨集   2004.3

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  • ウマ浅指屈筋腱におけるコラーゲン細線維の分岐

    渡辺 敬文, 保坂 善真, 植田 弘美, 竹花 一成

    日本獣医学会学術集会講演要旨集   2004.3

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  • ホルスタイン種育成牛の巣状性脂肪肝の1例:超音波診断の有用性

    Mohamed Tharwat, 及川 伸, 黒澤 隆, 竹花 一成, 保坂 善真, 岡田 洋之, 小岩 政照, 佐藤 博

    2004.3

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    食欲減退と呼吸器症状を示した30ヵ月齢のホルスタイン種牛に超音波検査を行った結果,肝門脈と胆嚢の間に高エコー領域の病変部が認められた.その病変部は,非球形で,隣接の血管の変位はなく,境界明瞭であった.病変部は組織学的に巣状性脂肪肝(FFL)と診断された. FFLの超音波所見は特徴的であるので,他の病変との類症鑑別診断に超音波検査は有用である.

    DOI: 10.1292/jvms.66.341

  • ホルスタイン種育成牛の巣状性脂肪肝の1例:超音波診断の有用性(短報)(Focal Fatty Liver in a Heifer: Utility of Ultrasonography in Diagnosis)

    Mohamed T, 及川 伸, 黒澤 隆, 竹花 一成, 保坂 善真, 岡田 洋之, 小岩 政照, 佐藤 博

    2004.3

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  • ホルスタイン種育成牛の巣状性脂肪肝の1例:超音波診断の有用性

    Mohamed Tharwat, 及川 伸, 黒澤 隆, 竹花 一成, 保坂 善真, 岡田 洋之, 小岩 政照, 佐藤 博

    2004.3

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    食欲減退と呼吸器症状を示した30ヵ月齢のホルスタイン種牛に超音波検査を行った結果,肝門脈と胆嚢の間に高エコー領域の病変部が認められた.その病変部は,非球形で,隣接の血管の変位はなく,境界明瞭であった.病変部は組織学的に巣状性脂肪肝(FFL)と診断された. FFLの超音波所見は特徴的であるので,他の病変との類症鑑別診断に超音波検査は有用である.

  • 腱組織の取込み能について

    保坂 善真, 山本 悦子, 植田 弘美, 竹花 一成

    日本獣医学会学術集会講演要旨集   2003.9

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  • イヌ膝関節における前十字靱帯と外側側副靱帯の構造的差異

    松川 拓也, 山本 悦子, 保坂 善真, 植田 弘美, 中村 邦男, 竹花 一成

    日本獣医学会学術集会講演要旨集   2003.9

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  • ウマ浅指屈筋腱と総指伸筋腱の形態学的および生化学的性状の比較

    山本 悦子, Birch Helen L, Goodship Allen E, 渡邊 敬文, 保坂 善真, 植田 弘美, 竹花 一成

    日本獣医学会学術集会講演要旨集   2003.9

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  • ウマ浅指屈筋腱の部位差について

    瀬瀬 賢, 渡邊 敬文, 山本 悦子, 保坂 善真, 植田 弘美, 竹花 一成

    日本獣医学会学術集会講演要旨集   2003.9

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  • ウマ浅指屈腱炎におけるマトリックスメタロプロテアーゼ活性

    山本 悦子, 渡邊 敬文, 保坂 善真, 植田 弘美, 竹花 一成

    日本獣医学会学術集会講演要旨集   2003.9

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  • 馬浅指屈腱炎におけるマトリックスメタロプロテアーゼ活性

    山本 悦子, 渡辺 敬文, 保坂 善真, 植田 弘美, 竹花 一成

    北海道獣医師会雑誌   2003.8

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  • 犬の混濁を呈した角膜の形態学的及び生化学的解析

    廣岡 雅満, 五十嵐 治, 山本 悦子, 保坂 善真, 植田 弘美, 竹花 一成

    北海道獣医師会雑誌   2003.8

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  • 犬膝関節十字靱帯,側副靱帯の力学的,形態学的及び生化学的相異

    松川 拓也, 山本 悦子, 保坂 善真, 植田 弘美, 中村 邦男, 竹花 一成

    北海道獣医師会雑誌   2003.8

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  • 血管壁に発現する陰性荷電について

    植田 弘美, 山本 悦子, 保坂 善真, 竹花 一成

    解剖学雑誌   2003.4

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  • なぜウマの屈腱炎は骨腱付着部で発症しないのか?

    佐藤 紀子, 保坂 善真, 植田 弘美, 竹花 一成

    解剖学雑誌   2003.4

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  • シロザケ(Oncorhynchus keta)卵表面の塩漬に対する形態学的変化

    横澤 香菜, 保坂 善真, 植田 弘美, 武田 浩郁, 竹花 一成

    解剖学雑誌   2003.4

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  • 淡水カメへのビタミンD3投与による鰓後体と上皮小体の応答

    荒木 貴裕, 保坂 善真, 植田 弘美, 竹花 一成

    解剖学雑誌   2003.4

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  • 腱細胞におけるTNFα細胞内シグナル伝達因子の発現

    保坂 善真, 桐沢 力雄, 岩永 敏彦, 山本 悦子, 植田 弘美, 竹花 一成

    解剖学雑誌   2003.4

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  • 腱炎における腫瘍壊死因子(TNF)αの関与

    保坂 善真, 桐沢 力雄, 岩永 敏彦, 山本 悦子, 植田 弘美, 竹花 一成

    日本獣医学会学術集会講演要旨集   2003.3

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  • ウマ浅指屈筋腱の加齢性変化に関する研究

    山本 悦子, 保坂 善真, 植田 弘美, ほか

    日本獣医師会雑誌 = Journal of the Japan Veterinary Medical Association   2003.2

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    サラブレッド種における浅指屈筋腱の加齢に伴う形態学的および生化学的変化に関する詳細な報告はなされていない。今回、浅指屈筋腱の加齢に伴う形態学的および生化学的変化を明らかにした。形態学的変化として、腱を構成するコラーゲン細線維の配列は、加齢に伴って波状構造が消失し直線的になった。またコラーゲン細線維の直径分布は加齢に伴って細い細線維の割合が増加した。生化学的変化として、コラーゲンの型は加齢に伴ってⅢ型コラーゲンが増加していた。またグリコサミノグリカンは、コラーゲン細線維の直径調節作用を有するデルマタン硫酸が加齢に伴って減少し、コンドロイチン硫酸が増加した。このことから、サラブレッド種の浅指屈筋腱の加齢に伴う形態学的変化と生化学的変化には関連性があることが明らかとなった。

  • ウマ浅指屈筋腱の加齢性変化に関する研究

    山本 悦子, 保坂 善真, 植田 弘美, 中村 富美男, 竹花 一成

    日本獣医師会雑誌 = Journal of the Japan Veterinary Medical Association   2003.2

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    サラブレッド種における浅指屈筋腱の加齢に伴う形態学的および生化学的変化に関する詳細な報告はなされていない.今回, 浅指屈筋腱の加齢に伴う形態学的および生化学的変化を明らかにした.形態学的変化として, 腱を構成するコラーゲン細線維の配列は, 加齢に伴って波状構造が消失し直線的になった.またコラーゲン細線維の直径分布は加齢に伴って細い細線維の割合が増加した.生化学的変化として, コラーゲンの型は加齢に伴ってIII型コラーゲンが増加していた.またグリコサミノグリカンは, コラーゲン細線維の直径調節作用を有するデルマタン硫酸が加齢に伴って減少し, コンドロイチン硫酸が増加した.このことから, サラブレッド種の浅指屈筋腱の加齢に伴う形態学的変化と生化学的変化には関連性があることが明らかとなった.

  • 馬浅指屈筋腱の腱細胞におけるサイトカインの局在(短報)

    保坂 善真, 桐沢 力雄, 山本 悦子, 植田 弘美, 岩井 浤, 竹花 一成

    2002.10

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    馬浅指屈筋腱における4種類のサイトカイン(IL-1α,IL-1β,TNFαおよびIFNγ)の局在を免疫組織化学的手法で検索した.炎症腱では腱細胞は用いた全てのサイトカイン抗体に陽性で,腱内膜細胞と血管内皮細胞も陽性を示したが,正常腱では腱細胞は陰性を示した.腱細胞の反応性が炎症の有無およびサイトカイン種によって異なったことは,浅指屈筋腱の生理的,病理的状態を反映しているものと推察された.

  • 馬浅指屈筋腱の腱細胞におけるサイトカインの局在(短報)

    保坂 善真, 桐沢 力雄, 山本 悦子, 植田 弘美, 岩井 浤, 竹花 一成

    2002.10

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    馬浅指屈筋腱における4種類のサイトカイン(IL-1α,IL-1β,TNFαおよびIFNγ)の局在を免疫組織化学的手法で検索した.炎症腱では腱細胞は用いた全てのサイトカイン抗体に陽性で,腱内膜細胞と血管内皮細胞も陽性を示したが,正常腱では腱細胞は陰性を示した.腱細胞の反応性が炎症の有無およびサイトカイン種によって異なったことは,浅指屈筋腱の生理的,病理的状態を反映しているものと推察された.

    DOI: 10.1292/jvms.64.945

  • 馬浅指屈筋腱の腱細胞におけるサイトカインの局在(Localization of Cytokines in Tendinocytes of the Superficial Digital Flexor Tendon in the Horse)

    保坂 善真, 桐沢 力雄, 山本 悦子, 植田 弘美, 岩井 浤, 竹花 一成

    2002.10

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  • 腱における炎症性サイトカインとアポトーシス関連シグナル伝達因子の発現

    保坂 善真, 桐沢 力雄, 岩永 敏彦, 山本 悦子, 坂本 由梨恵, 植田 弘美, 竹花 一成

    日本獣医学会学術集会講演要旨集   2002.8

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  • ウマの浅指屈腱筋におけるゴルジ腱紡錘について

    渡辺 敬文, 保坂 善真, 植田 弘美, 竹花 一成

    日本獣医学会学術集会講演要旨集   2002.8

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  • ラットの膵臓B細胞分泌に対する酢酸投与の影響

    久松 和加, 保坂 善真, 植田 弘美, 竹花 一成

    日本獣医学会学術集会講演要旨集   2002.8

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  • 腱組織における炎症性サイトカインとアポトーシス発現の関連 ウマ浅指屈腱における解析

    保坂 善真, 山本 悦子, 植田 弘美, 竹花 一成

    2002.4

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  • ウマ腱細胞におけるアポトーシス発現とサイトカインの関与について

    保坂 善真, 山本 悦子, 植田 弘美, 竹花 一成

    解剖学雑誌   2002.3

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  • イヌ子宮蓄膿症における子宮内膜の形態学的及び生化学的性状

    有泉 幸雄, 山本 悦子, 保坂 善真, 植田 弘美, 竹花 一成

    日本獣医学会学術集会講演要旨集   2001.9

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Professional Memberships

  • THE JAPANESE SOCIETY OF CARBOHYDRATE RESEARCH

  • THE JAPANESE ASSOCIATION OF ANATOMISTS

  • THE JAPANESE SOCIETY OF VETERINARY SCIENCE

  • JAPANESE SOCIETY OF ANIMAL SCIENCE

  • Warm Regional Society of Animal Science, Japan

  • THE JAPANESE SOCIETY OF VETERINARY SCIENCE

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  • JAPANESE SOCIETY OF ANIMAL SCIENCE

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  • THE JAPANESE SOCIETY OF CARBOHYDRATE RESEARCH

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  • THE JAPANESE ASSOCIATION OF ANATOMISTS

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Committee Memberships

  • Executive   Domestic

    2017.7 - 2033.3   

  • Councilor   Domestic

    2014.4 - 2033.3   

  • Councilor   Domestic

    2008.4 - 2033.3   

Academic Activities

  • Journal of Veterinary Medical Science International contribution

    2020.10 - 2024.12

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    Type:Academic society, research group, etc. 

  • Japanese Journal of Veterinary Research International contribution

    2020.10 - 2023.5

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  • 日本学術振興会 特別研究員等審査会専門委員(書面担当)

    Role(s): Review, evaluation

    日本学術振興会  2017.6 - 2019.5

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    Type:Scientific advice/Review 

Research Projects

  • ウシ子宮内膜の細胞外マトリックスの発現動態をオルガノイドを用いて解析する

    2024

    2024年度 旗影会研究助成

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    Authorship:Principal investigator  Grant type:Contract research

  • 採卵鶏雄雛の新たな可能性:発育、肉質、行動からみた経済的有効性の探求

    2024

    令和6年度畜産関係学術研究委託調査

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    Authorship:Principal investigator  Grant type:Contract research

  • ホルモンと糖鎖から解き明かす骨格筋間質の機能維持機構

    Grant number:23K27078  2023.4 - 2026.3

    科学研究費助成事業  基盤研究(B)

    保坂 善真, 田村 純一, 岩崎 智仁, 尾嶋 孝一

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    骨格筋組織の間質は筋力伝達に重要だが、低エストロゲン状態では脂肪化や線維化が生じやすく、筋量や筋力低下の原因になる。間質内の間葉系前駆細胞(MPC)は、E2によってCS産生を変化させ、成長因子の制御をしていると考えているが、分子基盤は不明である。今年度は、ERの遺伝子発現を抑制したMPCを用いて、CS産生に関与するERを同定する。また、CSと成長因子の結合の解析ならびにCS合成酵素の発現調節細胞による解析も行い、MPC分化制御メカニズムの統合的理解を目指す。

    CiNii Research

  • ホルモンと糖鎖から解き明かす骨格筋間質の機能維持機構

    Grant number:23H02385  2023 - 2025

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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  • ホルモンと糖鎖から解き明かす骨格筋間質の機能維持機構

    Grant number:23H02385  2023 - 2025

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • ウシ卵管でのグリコサミノグリカンの時間的・空間的変動の理解

    2023

    2023年度 旗影会研究助成

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  • Identification of chondroitin sulfate isotypes with adipocyte differentiation inhibitory activity, elucidation of their functions, and regulation of fat mass

    Grant number:20K06411  2020 - 2022

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    HOSAKA Yoshinao

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    Focusing on the relationship between adipocyte differentiation and chondroitin sulfate (CS), the following experiments were conducted. 1) Induced the differentiation of progenitor adipocyte 3T3-L1 into adipocytes in a medium containing CS, and attempted to identify CS species with high adipogenic differentiation inhibitory activity. As a result, C type rich CS showed strong inhibitory activity against adipogenesis. 2) Analyzed in detail the changes in CS chains before and after differentiation into adipocytes, and found that the amount of chains, the CS species constituting them, and their proportions changed. Furthermore, the average chain length also became shorter after differentiation, indicating that qualitative and quantitative changes in the CSs surrounding the cells occurred with the progress of adipocyte differentiation.

    CiNii Research

  • Exploring predictive factors to identify statin-sensitivity of cancer cells and building evidence for cancer metastasis inhibitors

    Grant number:19H03514  2019 - 2022

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Warita Katsuhiko

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    Grant type:Scientific research funding

    Statins are drugs used for hyperlipidemia and are expected to be used in cancer therapy from the viewpoint of drug repurposing (also termed drug repositioning). In this study, we used statin-sensitive and -resistant cancer cell lines to analyze (1) relationship between epithelial-mesenchymal transition (EMT) and statin sensitivity, (2) metabolic systems involved in statin sensitivity, and (3) effects on cell motility involved in cancer metastasis. As a result, EMT increased statin sensitivity of cancer cells. Also, statin-sensitive and -resistant cancer cell lines showed significantly different metabolic profiles after statin treatment, and these differences emerged as candidate factors related to statin sensitivity. The results also suggest that statins may exhibit anti-cancer effects in statin-sensitive cancer cells, even under hypoxic conditions, where drug resistance and invasive metastatic potential are enhanced.

    CiNii Research

  • 脳弓下器官のアンジオテンシンII誘発性持続性活性は生理的調節機構か病態の発端か

    Grant number:19K06403  2019 - 2021

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Grant type:Scientific research funding

  • 末梢感覚神経レベルでの疼痛緩和システムの解明

    Grant number:17H03933  2017 - 2020

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Grant type:Scientific research funding

  • ホルモンと糖鎖から迫る骨格筋組織の質的・量的制御のメカニズム

    Grant number:17H03934  2017 - 2019

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Grant type:Scientific research funding

  • 骨格筋発達の統合的理解を目指す異種細胞間コミュニケーション機構の全容解明

    Grant number:16H02585  2016 - 2018

    日本学術振興会  科学研究費助成事業  基盤研究(A)

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    Grant type:Scientific research funding

  • 中枢性浸透圧感知における新規TRPV1分子の機能解析

    Grant number:16K08073  2016 - 2018

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Grant type:Scientific research funding

  • スタチン系薬剤の制がん機構の解明と抗腫瘍効果を予測する新たな評価法への展開

    Grant number:16K18439  2016 - 2018

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Grant type:Scientific research funding

  • 骨格筋分化を制御するコンドロイチン硫酸種の同定と機能の解明

    Grant number:26450444  2014 - 2016

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Grant type:Scientific research funding

  • 癌細胞のスタチン系薬剤感受性を決定する分子メカニズム―感受性マーカーの探索―

    Grant number:26890019  2014 - 2015

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Grant type:Scientific research funding

  • 骨格筋における異種細胞間コミュニケーション機構の解明

    Grant number:24248045  2012 - 2014

    日本学術振興会  科学研究費助成事業  基盤研究(A)

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    Grant type:Scientific research funding

  • 脂肪細胞はなぜ筋肉内に脂肪組織を構築できるのか?-霜降り肉形成機構解明への挑戦-

    Grant number:24658222  2012 - 2013

    日本学術振興会  科学研究費助成事業  挑戦的研究(萌芽)

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    Grant type:Scientific research funding

  • 骨リモデリングを調節するコンドロイチン硫酸種の同定と機能の解析・骨量の制御

    Grant number:23780293  2011 - 2013

    科学研究費助成事業  若手研究(A,B)

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    Grant type:Scientific research funding

  • 犬と猫の腎疾患におけるCOXとRA系を介した新規病態解明と診断治療法開発

    Grant number:22580364  2010 - 2012

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Grant type:Scientific research funding

  • 眼球の組織発生とサイズ決定に関わるコンドロイチン硫酸種の同定と機能解析

    Grant number:22590174  2010 - 2012

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Grant type:Scientific research funding

  • 間葉系幹細胞の骨分化に関わるデコリン糖鎖種の同定・機能解析および骨組織再生

    2009 - 2011

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Grant type:Scientific research funding

  • 筋発達制御因子・ミオスタチンのデコリンによる調節機構

    Grant number:21380176  2009 - 2011

    日本学術振興会  科学研究費助成事業  基盤研究(B)

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    Grant type:Scientific research funding

  • 間葉系幹細胞の骨分化に関わるデコリン糖鎖種の同定・機能解析と骨組織再生

    Grant number:21780265  2009 - 2010

    科学研究費助成事業  若手研究(A,B)

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    Grant type:Scientific research funding

  • ナマコ由来高硫酸化コンドロイチン硫酸の生理活性機能

    2009

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Scientific research funding

  • 筋細胞増殖分化過程でのデコリンの生理活性機能

    2008

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Grant type:Scientific research funding

  • 間葉系幹細胞から運動器へ分化する過程でのデコリンの関与と機能の解析

    Grant number:19780220  2007 - 2008

    科学研究費助成事業  若手研究(A,B)

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    Grant type:Scientific research funding

  • 間葉系幹細胞から運動器へ分化する過程でのデコリンの機能と役割の解明

    2007 - 2008

    日本学術振興会  科学研究費助成事業  基盤研究(C)

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    Grant type:Scientific research funding

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Class subject

  • 動物組織学Ⅱ

    2024.12 - 2025.2   Winter quarter

  • 動物形態学Ⅱ

    2024.12 - 2025.2   Winter quarter

  • 動物組織学Ⅰ

    2024.10 - 2024.12   Fall quarter

  • 動物形態形成学特論

    2024.10 - 2024.12   Fall quarter

  • 動物形態学Ⅰ

    2024.10 - 2024.12   Fall quarter

  • 卒業研究(アニマルサイエンス分野)

    2024.4 - 2025.3   Full year

  • 科学英語(アニマルサイエンス分野)

    2024.4 - 2024.9   First semester

  • 家畜衛生学

    2024.4 - 2024.9   First semester

  • 家畜生体機構学実験

    2024.4 - 2024.9   First semester

  • 動物組織学Ⅱ

    2023.12 - 2024.2   Winter quarter

  • 動物形態形成学特論

    2023.10 - 2023.12   Fall quarter

  • 動物組織学Ⅰ

    2023.10 - 2023.12   Fall quarter

  • 家畜生体機構学Ⅱ

    2023.6 - 2023.8   Summer quarter

  • 家畜生体機構学実験

    2023.4 - 2023.9   First semester

  • 農学入門Ⅰ

    2023.4 - 2023.9   First semester

  • 家畜生体機構学Ⅰ

    2023.4 - 2023.6   Spring quarter

  • 動物組織学Ⅱ

    2022.12 - 2023.2   Winter quarter

  • 農学入門Ⅱ

    2022.10 - 2023.3   Second semester

  • 動物組織学Ⅰ

    2022.10 - 2022.12   Fall quarter

  • 動物組織学Ⅱ

    2024.12 - 2025.2   Winter quarter

  • 動物形態学Ⅱ

    2024.12 - 2025.2   Winter quarter

  • 実地見学(アニマルサイエンス分野)

    2024.10 - 2025.3   Second semester

  • 動物組織学Ⅰ

    2024.10 - 2024.12   Fall quarter

  • 動物形態学Ⅰ

    2024.10 - 2024.12   Fall quarter

  • 動物形態形成学特論

    2024.10 - 2024.12   Fall quarter

  • 卒業研究(アニマルサイエンス分野)

    2024.4 - 2025.3   Full year

  • 科学英語(アニマルサイエンス分野)

    2024.4 - 2024.9   First semester

  • 家畜生体機構学実験

    2024.4 - 2024.9   First semester

  • 家畜衛生学

    2024.4 - 2024.9   First semester

▼display all

Visiting, concurrent, or part-time lecturers at other universities, institutions, etc.

  • 2022  鳥取大学  Classification:Part-time lecturer  Domestic/International Classification:Japan 

Other educational activity and Special note

  • 2024  Class Teacher 

Outline of Social Contribution and International Cooperation activities

  • 学会誌編集委員
    The Journal of Veterinary Medical Science編集委員(解剖学分野) 2020年7月〜
    Japanese Journal of Veterinary Research編集委員(Cell Biology分野) 2017年10月〜

Travel Abroad

  • 2006.10 - 2007.9

    Staying countory name 1:Singapore   Staying institution name 1:Institute of Molecular Cell Biology, Singapore