Language：English   Publishing type：Research paper (scientific journal)   Publisher：Aging Cell  

In response to peroxynitrite (ONOO-) generation, myogenic stem satellite cell activator HGF (hepatocyte growth factor) undergoes nitration of tyrosine residues (Y198 and Y250) predominantly on fast IIa and IIx myofibers to lose its binding to the signaling receptor c-met, thereby disturbing muscle homeostasis during aging. Here we show that rat anti-HGF monoclonal antibody (mAb) 1H41C10, which was raised in-house against a synthetic peptide FTSNPEVRnitroY198EV, a site well-conserved in mammals, functions to confer resistance to nitration dysfunction on HGF. 1H41C10 was characterized by recognizing both nitrated and non-nitrated HGF with different affinities as revealed by Western blotting, indicating that the paratope of 1H41C10 may bind to the immediate vicinity of Y198. Subsequent experiments showed that 1H41C10-bound HGF resists peroxynitrite-induced nitration of Y198. A companion mAb-1H42F4 presented similar immuno-reactivity, but did not protect Y198 nitration, and thus served as the control. Importantly, 1H41C10-HGF also withstood Y250 nitration to retain c-met binding and satellite cell activation functions in culture. The Fab region of 1H41C10 exerts resistivity to Y250 nitration possibly due to its localization in the immediate vicinity to Y250, as supported by an additional set of experiments showing that the 1H41C10-Fab confers Y250-nitration resistance which the Fc segment does not. Findings highlight the in vitro preventive impact of 1H41C10 on HGF nitration-dysfunction that strongly impairs myogenic stem cell dynamics, potentially pioneering cogent strategies for counteracting or treating age-related muscle atrophy with fibrosis (including sarcopenia and frailty) and the therapeutic application of investigational HGF drugs.

DOI： 10.1111/acel.14337

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：In Vitro Cellular and Developmental Biology - Animal  

Skeletal muscle tissue increases or decreases its volume by synthesizing or degrading myofibrillar proteins. The ubiquitin-proteasome system plays a pivotal role during muscle atrophy, where muscle ring finger proteins (Murf) function as E3 ubiquitin ligases responsible for identifying and targeting substrates for degradation. Our previous study demonstrated that overexpression of Ozz, an E3 specific to embryonic myosin heavy chain (Myh3), precisely reduced the Myh3 replacement rate in the thick filaments of myotubes (E. Ichimura et al., Physiol Rep. 9:e15003, 2021). These findings strongly suggest that E3 plays a critical role in regulating myosin replacement. Here, we hypothesized that the Murf isoforms, which recognize Myhs as substrates, reduced the myosin replacement rates through the enhanced Myh degradation by Murfs. First, fluorescence recovery after a photobleaching experiment was conducted to assess whether Murf isoforms affected the GFP-Myh3 replacement. In contrast to Murf2 or Murf3 overexpression, Murf1 overexpression selectively facilitated the GFP-Myh3 myosin replacement. Next, to examine the effects of Murf1 overexpression on the replacement of myosin isoforms, Cherry-Murf1 was coexpressed with GFP-Myh1, GFP-Myh4, or GFP-Myh7 in myotubes. Intriguingly, Murf1 overexpression enhanced the myosin replacement of GFP-Myh4 but did not affect those of GFP-Myh1 or GFP-Myh7. Surprisingly, overexpression of Murf1 did not enhance the ubiquitination of proteins. These results indicate that Murf1 selectively regulated myosin replacement in a Myh isoform-dependent fashion, independent of enhanced ubiquitination. This suggests that Murf1 may have a role beyond functioning as a ubiquitin ligase E3 in thick filament myosin replacement.

DOI： 10.1007/s11626-024-00916-0

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Eicosapentaenoic acid increases proportion of type 1 muscle fibers through PPARδ and AMPK pathways in rats

DOI： 10.1016/j.isci.2024.109816

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Language：Japanese   Publisher：Japanese Society of Animal Science  

<p>日本畜産学会若手企画委員会は，分野や所属の垣根を越えた若手研究者のネットワーク構築および今後のキャリアパスの参考となる様々な企画を実施している．そして，若手研究者の奮起と成長を促すことで，今後の畜産学および畜産業の発展に貢献することを目的とする．本稿では，2023年9月19日の第131回日本畜産学会大会において，4年ぶりに対面開催された「第26回若手企画シンポジウム　どうする？どうなる！？日本の畜産業界　〜現状と現場を知る〜」について，簡単に紹介する．</p>

DOI： 10.2508/chikusan.95.103

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Language：Others   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

C-C motif chemokine ligand 2 regulates prostaglandin synthesis and embryo attachment of the bovine endometrium during implantation

DOI： 10.1007/s00441-024-03869-8

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Other Link： https://link.springer.com/article/10.1007/s00441-024-03869-8/fulltext.html

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Aging Cell  

Mechanical perturbation triggers activation of resident myogenic stem cells to enter the cell cycle through a cascade of events including hepatocyte growth factor (HGF) release from its extracellular tethering and the subsequent presentation to signaling-receptor c-met. Here, we show that with aging, extracellular HGF undergoes tyrosine-residue (Y) nitration and loses c-met binding, thereby disturbing muscle homeostasis. Biochemical studies demonstrated that nitration/dysfunction is specific to HGF among other major growth factors and is characterized by its locations at Y198 and Y250 in c-met-binding domains. Direct-immunofluorescence microscopy of lower hind limb muscles from three age groups of rat, provided direct in vivo evidence for age-related increases in nitration of ECM-bound HGF, preferentially stained for anti-nitrated Y198 and Y250-HGF mAbs (raised in-house) in fast IIa and IIx myofibers. Overall, findings highlight inhibitory impacts of HGF nitration on myogenic stem cell dynamics, pioneering a cogent discussion for better understanding age-related muscle atrophy and impaired regeneration with fibrosis (including sarcopenia and frailty).

DOI： 10.1111/acel.14041

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Language：Others   Publishing type：Research paper (scientific journal)   Publisher：Scientific Reports  

Abstract

Skeletal muscle is one of the largest metabolic tissues in mammals and is composed of four different types of muscle fibers (types 1, 2A, 2X, and 2B); however, type 2B is absent in humans. Given that slow-twitch fibers are superior to fast-twitch fibers in terms of oxidative metabolism and are rich in mitochondria, shift of muscle fiber types in direction towards slower fiber types improves metabolic disorders and endurance capacity. We previously had reported that oleic acid supplementation increases type 1 fiber formation in C2C12 myotubes; however, its function still remains unclear. This study aimed to determine the effect of oleic acid on the muscle fiber types and endurance capacity. An in vivo mouse model was used, and mice were fed a 10% oleic acid diet for 4 weeks. Two different skeletal muscles, slow soleus muscle with the predominance of slow-twitch fibers and fast extensor digitorum longus (EDL) muscle with the predominance of fast-twitch fibers, were used. We found that dietary oleic acid intake improved running endurance and altered fiber type composition of muscles, the proportion of type 1 and 2X fibers increased in the soleus muscle and type 2X increased in the EDL muscle. The fiber type shift in the EDL muscle was accompanied by an increased muscle TAG content. In addition, blood triacylglycerol (TAG) and non-esterified fatty acid levels decreased during exercise. These changes suggested that lipid utilization as an energy substrate was enhanced by oleic acid. Increased proliferator-activated receptor γ coactivator-1β protein levels were observed in the EDL muscle, which potentially enhanced the fiber type transitions towards type 2X and muscle TAG content. In conclusion, dietary oleic acid intake improved running endurance with the changes of muscle fiber type shares in mice. This study elucidated a novel functionality of oleic acid in skeletal muscle fiber types. Further studies are required to elucidate the underlying mechanisms. Our findings have the potential to contribute to the field of health and sports science through nutritional approaches, such as the development of supplements aimed at improving muscle function.

DOI： 10.1038/s41598-023-50464-y

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Journal of Pathology  

α-Klotho is a longevity-related protein. Its deficiency shortens lifespan with prominent senescent phenotypes, including muscle atrophy and weakness in mice. α-Klotho has two forms: membrane α-Klotho and circulating α-Klotho (c-α-Klotho). Loss of membrane α-Klotho impairs a phosphaturic effect, thereby accelerating phosphate-induced aging. However, the mechanisms of senescence on c-α-Klotho loss remain largely unknown. Herein, with the aging of wild-type mice, c-α-Klotho declined, whereas Smad2, an intracellular transforming growth factor (TGF)-β effector, became activated in skeletal muscle. Moreover, c-α-Klotho suppressed muscle-wasting TGF-β molecules, including myostatin, growth and differentiation factor 11, activin, and TGF-β1, through binding to ligands as well as type I and type II serine/threonine kinase receptors. Indeed, c-α-Klotho reversed impaired in vitro myogenesis caused by these TGF-βs. Oral administration of Ki26894, a small-molecule inhibitor of type I receptors for these TGF-βs, restored muscle atrophy and weakness in α-Klotho (-/-) mice and in elderly wild-type mice by suppression of activated Smad2 and up-regulated Cdkn1a (p21) transcript, a target of phosphorylated Smad2. Ki26894 also induced the slow to fast myofiber switch. These findings show c-α-Klotho's potential as a circulating inhibitor counteracting TGF-β-induced sarcopenia. These data highlight the potential of a novel therapy involving TGF-β blockade to prevent sarcopenia.

DOI： 10.1016/j.ajpath.2023.01.009

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Language：English   Publisher：Biochemistry and Biophysics Reports  

Protein tyrosine residue (Y) nitration, a post-translational chemical-modification mode, has been associated with changes in protein activity and function; hence the accumulation of specific nitrated proteins in tissues may be used to monitor the onset and progression of pathological disorders. To verify the possible impact of nitration on postnatal muscle growth and regeneration, a pilot study was designed to examine the nitration/dysfunction of hepatocyte growth factor (HGF), a key ligand that is released from the extracellular tethering and activates myogenic stem satellite cells to enter the cell cycle upon muscle stretch and injury. Exposure of recombinant HGF (a hetero-dimer of α- and β-chains) to peroxynitrite induces Y nitration in HGF α-chain under physiological conditions. Physiological significance of this finding was emphasized by Western blotting that showed the NK1 segment of HGF (including a K1 domain critical for signaling-receptor c-met binding) undergoes nitration with a primary target of Y198. Peroxynitrite treatment abolished HGF-agonistic activity of the NK1 segment, as revealed by in vitro c-met binding and bromodeoxyuridine-incorporation assays. Importantly, direct-immunofluorescence microscopy of rat lower hind-limb muscles from two aged-groups (2-month-old “young” and 12-month-old “retired/adult”) provided in vivo evidence for age-related nitration of extracellular HGF (Y198). Overall, findings provide the insight that HGF/NK1 nitration/dysfunction perturbs myogenic stem cell dynamics and homeostasis; hence NK1 nitration may stimulate progression of muscular disorders and diseases including sarcopenia.

DOI： 10.1016/j.bbrep.2022.101295

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：American journal of physiology. Cell physiology  

Skeletal muscle consists of slow and fast myofibers in which different myosin isoforms are expressed. Approximately 300 myosins form a single thick filament in the myofibrils, where myosin is continuously exchanged. However, endogenous slow and fast myosin dynamics have not been fully understood. To elucidate those dynamics, here we generated mice expressing green fluorescence protein-tagged slow myosin heavy chain (GFP-Myh7) and Kusabira Orange fluorescence protein-tagged fast myosin heavy chain (KuO-Myh1). First, these mice enabled us to distinguish between GFP- and KuO-myofibers under fluorescence microscopy: GFP-Myh7 and KuO-Myh1 were exclusively expressed in slow myofibers and fast myofibers, respectively. Next, to monitor endogenous myosin dynamics, fluorescence recovery after photobleaching (FRAP) was conducted. The mobile fraction (Mf) of GFP-Myh7 and that of KuO-Myh1 were almost constant values independent of the regions of the myofibers and the muscle portions where the myofibers were isolated. Intriguingly, proteasome inhibitor treatment significantly decreased the Mf in GFP-Myh7 but not in KuO-Myh1 myofibers, indicating that the response to a disturbance in protein turnover depended on muscle fiber type. Taken together, the present results indicated that the mice we generated are promising tools not only for distinguishing between GFP- and KuO-myofibers but also for studying the dynamics of endogenous myosin isoforms by live-cell fluorescence imaging.

DOI： 10.1152/ajpcell.00415.2021

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japan Poultry Science Association  

<p>Smooth muscle cells are widely distributed in the digestive organs of chickens. They exist as single cells, but adhere to each other to function synchronously. In this study, the expression of the gap junction protein connexin 43 (Cx43) in chicken gizzards was investigated at embryonic days (E) 10, E15, and E18. Gizzards have an abundance of smooth muscle cells because of their thick muscle layers, which enable easy analysis of the cells. Morphological observations and expression patterns of smooth muscle markers were confirmed. Next, we observed where the markers were localized in the gizzard tissue at E10, E15, and E18. Finally, the expression pattern of Cx43 in primary cultured smooth muscle cells from E15 gizzards was investigated. The analysis revealed the expression and localization of Cx43 and calponin 1 in the smooth muscle layers, and 3D analysis revealed dynamic changes in the localization pattern of Cx43 from E10 to E15. Cultured smooth muscle cells confirmed that Cx43 was expressed in the cell membrane and cytosol. In conclusion, Cx43 expression was identified in chicken gizzards at E10, E15, and E18, which was localized differently during development. The expression was broad at E10, and became restricted at E15 and E18. Primary culture of smooth muscle cells showed that Cx43 was present in the cell membrane and cytosol. This suggests that Cx43 is actively translated into the cytosol at E10, forming a hexamer, and shuttling the cell membrane to function as a gap junction.</p>

DOI： 10.2141/jpsa.0220003

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DOI： 10.14814/phy2.15003

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<title>Abstract</title>
Although skeletal muscle cells and adipocytes are derived from the same mesoderm, they do not transdifferentiate in vivo and are strictly distinct at the level of gene expression. To elucidate some of the regulatory mechanisms underlying this strict distinction, Pax7, a myogenic factor, was ectopically expressed in 3T3-L1 adipose progenitor cells to perturb their adipocyte differentiation potential. Transcriptome analysis showed that ectopic expression of Pax7 repressed the expression of some adipocyte genes and induced expression of some skeletal muscle cell genes. We next profiled the epigenomic state altered by Pax7 expression using H3K27ac, an activating histone mark, and H3K27me3, a repressive histone mark, as indicators. Our results show that ectopic expression of Pax7 did not result in the formation of H3K27ac at loci of skeletal muscle-related genes, but instead resulted in the formation of H3K27me3 at adipocyte-related gene loci. These findings suggest that the primary function of ectopic Pax7 expression is the formation of H3K27me3, and muscle gene expression results from secondary regulation.

DOI： 10.1093/jb/mvab030

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DOI： 10.1007/s00441-020-03344-0

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DOI： 10.1016/j.phymed.2020.153360

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DOI： 10.1002/mnfr.202000853

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DOI： 10.1007/s10911-020-09457-0

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DOI： 10.1021/acs.jafc.0c01288

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DOI： 10.1016/j.bbrc.2020.02.099

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DOI： 10.1111/asj.13355

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DOI： 10.1016/j.tiv.2019.104741

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DOI： 10.1002/adbi.201900187

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DOI： 10.1007/s00441-020-03168-y

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IL-1β directly inhibits milk lipid production in lactating mammary epithelial cells concurrently with enlargement of cytoplasmic lipid droplets.

DOI： 10.1016/j.yexcr.2018.06.038

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HSP90 modulates 1 the myosin replacement rate in myofibrils.

DOI： 10.1152/ajpcell.00245.2017

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DOI： 10.1007/s10911-018-9393-3

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Study of dietary fat and skeletal muscle fiber type.

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The data presented in this article are related to the research articles entitled “APOBEC2 negatively regulates myoblast differentiation in muscle regeneration” and “Data supporting possible implication of APOBEC2 in self-renewal functions of myogenic stem satellite cells: toward understanding the negative regulation of myoblast differentiation” (Ohtsubo et al., 2017a, 2017b) [1,2]. This article provides in vivo phenotypical data to show that Paired Box Transcription Factor 7 (Pax7)-positive cell number (per myofiber) is significantly lower in APOBEC2 (a member of apoB mRNA editing enzyme, catalytic polypeptide-like family)-knockout muscle than the control wild-type tissue at the same age of 8-wk-old in mice. The emerging results support an essential role for APOBEC2 in the self-renewal functions of myogenic stem satellite cells, namely the re-establishment of quiescent status after activation and proliferation of myoblasts.

DOI： 10.1016/j.dib.2018.02.063

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During lactation, mammary epithelial cells (MECs) form the blood-milk barrier by less-permeable tight junctions (TJs) to prevent the leakage of milk components. Phytoestrogens affect the proliferation, differentiation, and apoptosis of MECs. However, it remains unclear whether phytoestrogens are involved in the blood-milk barrier. Therefore, we investigated the influence of phytoestrogens (coumestrol, genistein, and daidzein) by using an in vitro mouse-MEC-culture model. The results showed that coumestrol and genistein changed the expression of TJ proteins (claudins-3 and -4 and occludin), weakened barrier function, and reduced beta-casein production. Daidzein also weakened barrier function without inhibiting beta-casein production. Additionally, coumestrol and genistein induced apoptosis in MECs. These results indicate that phytoestrogens weaken the blood-milk barrier by directly affecting TJs and the cellular viability of lactating MECs in different ways.

DOI： 10.1021/acs.jafc.7b04786

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In striated muscles, approximately 300 myosin molecules form a single thick filament in myofibrils. Each myosin is continuously displaced by another myosin to maintain the thick filament structure. Our previous study using a fluorescence recovery after photobleaching (FRAP) technique showed that the myosin replacement rate is decreased by inhibition of protein synthesis, but myosin is still exchangeable. This result prompted us to examine whether myosin in the cytoplasm is involved in myosin replacement in myofibrils. To address this, FRAP was measured in green fluorescent protein (GFP)-tagged myosin heavy chain 3 (Myh3) expressing myotubes that were treated with streptolysin-O (SLO), which forms pores specifically in the plasma membrane to induce leakage of cytoplasmic proteins. Our biochemical data demonstrated that the cytoplasmic myosin content was reduced in SLO-permeabilized semi-intact myotubes. Furthermore, FRAP experiments showed a sluggish substitution rate of GFP-Myh3 in SLO-permeabilized myotubes. Taken together, these results demonstrate that the myosin substitution rate is significantly reduced by a decreased amount of myosin in the cytoplasm and that cytoplasmic myosin contributes to myosin replacement in myofibrils.

DOI： 10.1111/asj.12826

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Scope: Isoflavones are a class of polyphonic compounds present in legumes and are called phytoestrogens because of their estrogen-like activity. Estrogen influences the behavior of mammary epithelial cells (MECs) during pregnancy and lactation. In this study, we investigated the direct influences of isoflavones and their metabolites in milk production ability of MECs.
Methods and results: Mouse MECs were cultured with prolactin and dexamethasone (glucocorticoid analog) to induce milk production ability. Subsequently, lactating MECs were treated with each isoflavone. Coumestrol, biochanin A, genistein, and formononetin decreased the intracellular and secreted beta-casein. On the other hand, p-ethylphenol, daidzein, and equol did not significantly influence beta-casein production at any concentration. Coumestrol, biochanin A and genistein down-regulated the mRNA expression of whey acidic protein (WAP), lactoferrin and alpha-lactalbumin. In contrast, p-ethylphenol, daidzein and equol up-regulated beta-casein and/or WAP with alpha-lactalbumin. Furthermore, coumestrol and genistein down-regulated the expression of prolactin receptor and signal transducer and activator of transcription 5 (STAT5) accompanied by a decrease in STAT5 phosphorylation.
Conclusion: Isoflavones and their metabolites influence the milk production ability of MECs through different interactions with prolactin/STAT5 signaling. Simultaneous intake of multiple isoflavones by consumption of legumes may induce promotive or adverse effects on lactating MECs.

DOI： 10.1002/mnfr.201700156

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Current research on skeletal muscle injury and regeneration highlights the crucial role of nerve-muscle interaction in the restoration of innervation during that process. Activities of muscle satellite or stem cells, recognized as the 'currency' of myogenic repair, have a pivotal role in these events, as shown by ongoing research. More recent investigation of myogenic signalling events reveals intriguing roles for semaphorin3A (Sema3A), secreted by activated satellite cells, in the muscle environment during development and regeneration. For example, Sema3A makes important contributions to regulating the formation of blood vessels, balancing bone formation and bone remodelling, and inflammation, and was recently implicated in the establishment of fibre-type distribution through effects on myosin heavy chain gene expression. This review highlights the active or potential contributions of satellite-cell-derived Sema3A to regulation of the processes of motor neurite ingrowth into a regenerating muscle bed. Successful restoration of functional innervation during muscle repair is essential; this review emphasizes the integrative role of satellite-cell biology in the progressive coordination of adaptive cellular and tissue responses during the injury-repair process in voluntary muscle.

DOI： 10.1111/brv.12286

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This paper provides in vitro phenotypical data to show that APOBEC2, a member of apoB mRNA editing enzyme, catalytic polypeptide-like family, may implicate in self-renewal functions of myogenic stem satellite cells, namely in the re-establishment of quiescent status after activation and proliferation of myoblasts in single-myofiber culture.

DOI： 10.1016/j.dib.2017.03.051

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Recently we found that the deficiency of APOBEC2, a member of apoB mRNA editing enzyme, catalytic polypeptide-like family, leads to a diminished muscle mass and increased myofiber with centrally-located nuclei known as dystrophic phenotypes. APOBEC2 expression is predominant in skeletal and cardiac muscles and elevated exclusively at the early-differentiation phase of wild-type (WT) myoblast cultures; however the physiological significance is still un-known. Here we show that APOBEC2 is a key negative regulator of myoblast differentiation in muscle regeneration. APOBEC2-knockout (A2KO) mice myoblast cultures displayed a normal morphology of primary myotubes along with earlier increase in fusion index and higher expression levels of myosin heavy chain (MyHC), myogenin and its cooperating factor MEF2C than WT myoblasts. Similar response was observable in APOBEC2-knockdown cultures of WT myoblasts that were transfected with the specific siRNA at the differentiation phase (not proliferation phase). Importantly, cardiotoxin-injured A2KO gastrocnemius muscle provided in vivo evidence by showing larger up-regulation of neonatal MyHC and myogenin and hence earlier regeneration of myofiber structures with diminished cross-sectional areas and minimal Feret diameters. Therefore, the findings highlight a promising role for APOBEC2 in normal progression of regenerative myogenesis at the early-differentiation phase upon muscle injury. (C) 2017 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.bioce1.2017.02.005

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Our previous studies demonstrated that an 8-week intake of 5% (w/w) apple polyphenol (APP) in the diet improves muscle endurance of young-adult rats. In order to identify a lower limit of the dietary contribution of APP to the effect, the experiments were designed for lower-dose supplementation (8-week feeding of 0.5% APP in AIN-93G diet) to 12-week-old male Sprague-Dawley rats. Results clearly showed that the 0.5% APP diet significantly up-regulates slower myosin-heavy-chain (MyHC) isoform ratios (IIx and IIa relative to total MyHC) and myoglobin expression in lower hind-limb muscles examined (P<0.05). There was a trend to increased fatigue resistance detected from measurements of relative isometric plantar-flexion force torque generated by a stimulus train delivered to the tibial nerve (F(98, 1372)=1.246, P=0.0574). Importantly, there was no significant difference in the animal body-phenotypes or locomotor activity shown as total moving distance in light and dark periods. Therefore, the present study encourages the notion that even low APP-intake may increase the proportions of fatigue-resistant myofibers, and has promise as a strategy for modifying performance in human sports and improving function in age-related muscle atrophy.

DOI： 10.1111/asj.12655

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Distinct roles of prolactin, epidermal growth factor, and glucocorticoids in beta-casein secretion pathway in lactating mammary epithelial cells
Beta-casein is a secretory protein contained in milk. Mammary epithelial cells (MECs) synthesize and secrete beta-casein during lactation. However, it remains unclear how the beta-casein secretion pathway is developed after parturition. In this study, we focused on prolactin (PRL), epidermal growth factor (EGF), and glucocorticoids, which increase in blood plasma and milk around parturition. MECs cultured with PRL, EGF and dexamethasone (DEX: glucocorticoid analog) developed the beta-casein secretion pathway. In the absence of PRL, MECs hardly expressed beta-casein. EGF enhanced the expression and secretion of beta-casein in the presence of PRL and DEX. DEX treatment rapidly increased secreted beta-casein concurrent with enhancing beta-casein expression. DEX also up-regulated the expression of SNARE proteins, such as SNAP-23, VAMP-8 and Syntaxin-12. Furthermore, PRL and DEX regulated the expression ratio of alpha(st-), beta- and kappa-casein. These results indicate that PRL, EGF and glucocorticoids have distinct roles in the establishment of beta-casein secretion pathway. (C) 2016 Elsevier Ireland Ltd. All rights reserved.

DOI： 10.1016/j.mce.2016.11.006

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Experiments reveal a novel mechanism to regulate myofiber types and its activation by functional food ingredients.

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DOI： 10.1186/s12860-016-0083-y

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DOI： 10.14814/phy2.12553

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A recent study demonstrated a positive effect of apple polyphenol (APP) intake on muscle endurance of young-adult animals. While an enhancement of lipid metabolism may be responsible, in part, for the improvement, the contributing mechanisms still need clarification. Here we show that an 8-week intake of 5% (w/w) APP in the diet, up-regulates two features related to fiber type: the ratio of myosin heavy chain (MyHC) type IIx/IIb and myoglobin protein expression in plantaris muscle of 9-week-old male Fischer F344 rats compared to pair-fed controls (P < 0.05). Results were demonstrated by our SDS-PAGE system specialized for MyHC isoform separation and western blotting of whole muscles. Animal-growth profiles (food intake, body-weight gain, and internal-organ weights) did not differ between the control and 5% APP-fed animals (n = 9/group). Findings may account for the increase in fatigue resistance of lower hind limb muscles, as evidenced by a slower decline in the maximum isometric planter-flexion torque generated by a 100-s train of electrical stimulation of the tibial nerve. Additionally, the fatigue resistance was lower after 8 weeks of a 0.5% APP diet than after 5% APP, supporting an APP-dose dependency of the shift in fiber-type composition. Therefore, the present study highlights a promising contribution of dietary APP intake to increasing endurance based on fiber-type composition in rat muscle. Results may help in developing a novel strategy for application in animal sciences, and human sports and age-related health sciences.

DOI： 10.1371/journal.pone.0134303

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Regenerative intramuscular motor-innervation is thought to reside in the spatiotemporal expression of axon-guidance molecules. Our previous studies showed that resident myogenic stem cells, satellite cells, up-regulate a secreted neural-chemorepellent semaphorin 3A (Sema3A) during the early-differentiation period, in response to hepatocyte growth factor (HGF) elevated in injured muscle. However, a paracrine source of the HGF release is still unknown. Very recently, we proposed a possible contribution of anti-inflammatory macrophages (CD206-positive M2) by showing that M2 cells infiltrate predominantly at the early-differentiation phase (3-5 days post-injury) and produce/secrete large amounts of HGF. However, in understanding this concept there still remains a critical need to examine if phagocytotic pro-inflammatory macrophages (CD86-positive M1), another activated-phenotype still present at the early-differentiation phase concerned, produce HGF upon muscle injury. The current immunocytochemical study demonstrated that the HGF expression is negative for M1 prepared from cardiotoxin-injured Tibialis anterior muscle at day 5, in contrast to the intense fluorescent-signal of M2 served as a positive control. This supplementary result advances our understanding of a spatiotemporal burst of HGF secretion from M2 populations (not M1) to impact Sema3A expression, which ensures a coordinated delay in attachment of motoneuron terminals onto damaged and generating fibers during the early phase of muscle regeneration.

DOI： 10.1111/asj.12264

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DOI： 10.1016/j.biocel.2014.05.032

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Autonomous determination of muscle fiber-types by semaphorin 3A secreted from resident myogenic stem cells.

Language：English   Publishing type：Research paper (scientific journal)  

Muscle damage induces massive macrophage infiltration of the injury site, in which activated pro-inflammatory and anti-inflammatory phenotypes (currently classified as M1 and M2, respectively) have been documented as distinct functional populations predominant at different times after the conventional acute injury by intramuscular injection of snake venoms (cardiotoxin, notexin) or chemicals (bupivacaine hydrochloride, barium chloride). The present study employed a muscle-crush injury model that may better reflect the physiologic damage and repair processes initiated by contusing a gastrocnemius muscle in the lower hind-limb of adult mice with hemostat forceps, and examined the time-course invasion of M1 and M2 macrophages during muscle regeneration by immunocytochemistry of CD197 and CD206 marker proteins. CD197-positive M1 macrophages were observed exclusively at 1-4 days after crush followed by the alternative prevalence of CD206-positive M2 at 7 days of myogenic differentiation, characterized by increasing levels of myogenin messenger RNA expression. Preliminary PCR analysis showed that M2 may produce hepatocyte growth factor (HGF) in culture, providing additional benefit to understanding that M2 populations actively promote regenerative myogenesis (muscle fiber repair) and moto-neuritogenesis (re-attachment of motoneuron terminals onto damaged fibers) through their time-specific infiltration and release of growth factor at the injury site early in muscle regeneration. © 2013 Japanese Society of Animal Science.

DOI： 10.1111/asj.12105

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/asj.12014

Language：English   Publishing type：Research paper (scientific journal)  

Time-coordinated prevalence of extracellular HGF, FGF2 and TGF-ss 3 in crush-injured skeletal muscle
Successful regeneration and remodeling of neuromuscular junctions are critical for restoring functional capacities and properties of skeletal muscle after damage, and axon-guidance molecules may be involved in the signaling that regulates such restoration. Recently, we found that early-differentiated satellite cells up-regulate a secreted neural chemorepellent Sema3A upon in vivo muscle-crush injury. The study also revealed that Sema3A expression is up-regulated in primary satellite-cell cultures in response to hepatocyte growth factor (HGF) and basic fibroblast growth factor (FGF2) and is prevented by transforming growth factor (TGF)-beta 2, 3. In order to verify the physiological significance of this regulation in vitro, the present study was designed to estimate the time-course of extracellular HGF, FGF2 and TGF-beta 3 concentrations after crush-injury of Gastrocnemius muscle in the rat lower hind-limb, using a combination of a non-homogenization/non-spin extraction of extracellular wound fluids and enhanced chemiluminescenceWestern blotting analyses. Results clearly demonstrated that active HGF and FGF2 are prevalent in 28?days post-crush, whereas active TGF-beta 3 increases after 12?days, providing a better understanding of the time-coordinated levels of HGF, FGF2 and TGF-beta 3 that drive regulation of Sema3A expression during regenerative intramuscular moto-neuritogenesis.

DOI： 10.1111/j.1740-0929.2012.01057.x

Language：English   Publishing type：Research paper (scientific journal)  

Hara M, Tabata K, Suzuki T, Do MQ, Mizunoya W, Nakamura M, Nishimura S, Tabata S, Ikeuchi Y, Sunagawa K, Anderson JE, Allen RE, Tatsumi R. Calcium influx through a possible coupling of cation channels impacts skeletal muscle satellite cell activation in response to mechanical stretch. Am J Physiol Cell Physiol 302: C1741-C1750, 2012. First published March 28, 2012; doi:10.1152/ajpcell.00068.2012.-When skeletal muscle is stretched or injured, satellite cells, resident myogenic stem cells positioned beneath the basal lamina of mature muscle fibers, are activated to enter the cell cycle. This signaling pathway is a cascade of events including calcium-calmodulin formation, nitric oxide (NO) radical production by NO synthase, matrix metalloproteinase activation, release of hepatocyte growth factor (HGF) from the extracellular matrix, and presentation of HGF to the receptor c-met, as demonstrated by assays of primary cultures and in vivo experiments. Here, we add evidence that two ion channels, the mechanosensitive cation channel (MS channel) and the long-lasting-type voltage-gated calcium-ion channel (L-VGC channel), mediate the influx of extracellular calcium ions in response to cyclic stretch in satellite cell cultures. When applied to 1-h stretch cultures with individual inhibitors for MS and L-VGC channels (GsMTx-4 and nifedipine, respectively) or with a less specific inhibitor (gadolinium chloride, Gd), satellite cell activation and upstream HGF release were abolished, as revealed by bromodeoxyuridine-incorporation assays and Western blotting of conditioned media, respectively. The inhibition was dose dependent with a maximum at 0.1 mu M (GsMTx-4), 10 mu M (nifedipine), or 100 mu M (Gd) and canceled by addition of HGF to the culture media; a potent inhibitor for transient-type VGC channels (NNC55-0396, 100 mu M) did not show any significant inhibitory effect. The stretch response was also abolished when calcium-chelator EGTA (1.8 mM) was added to the medium, indicating the significance of extracellular free calcium ions in our present activation model. Finally, cation/calcium channel dependencies were further documented by calcium-imaging analyses on stretched cells; results clearly demonstrated that calcium ion influx was abolished by GsMTx-4, nifedipine, and EGTA. Therefore, these results provide an additional insight that calcium ions may flow in through L-VGC channels by possible coupling with adjacent MS channel gating that promotes the local depolarization of cell membranes to initiate the satellite cell activation cascade.

DOI： 10.1152/ajpcell.00068.2012

Language：English   Publishing type：Research paper (scientific journal)  

Do MKQ, Sato Y, Shimizu N, Suzuki T, Shono J, Mizunoya W, Nakamura M, Ikeuchi Y, Anderson JE, Tatsumi R. Growth factor regulation of neural chemorepellent Sema3A expression in satellite cell cultures. Am J Physiol Cell Physiol 301: C1270-C1279, 2011. First published August 24, 2011; doi: 10.1152/ajpcell.00257.2011.-Successful regeneration and remodeling of the intramuscular motoneuron network and neuromuscular connections are critical for restoring skeletal muscle function and physiological properties. The regulatory signals of such coordination remain unclear, although axon-guidance molecules may be involved. Recently, satellite cells, resident myogenic stem cells positioned beneath the basal lamina and at high density at the myoneural junction regions of mature fibers, were shown to upregulate a secreted neural chemorepellent semaphorin 3A (Sema3A) in response to in vivo muscle-crush injury. The initial report on that expression centered on the observation that hepatocyte growth factor (HGF), an essential cue in muscle fiber growth and regeneration, remarkably upregulates Sema3A expression in early differentiated satellite cells in vitro [Tatsumi et al., Am J Physiol Cell Physiol 297: C238-C252, 2009]. Here, we address regulatory effects of basic fibroblast growth factor (FGF2) and transforming growth factor (TGF)-beta s on Sema3A expression in satellite cell cultures. When treated with FGF2, Sema3A message and protein were upregulated as revealed by reverse transcription-polymerase chain reaction and immunochemical studies. Sema3A upregulation by FGF2 was dose dependent with a maximum (8- to 1-fold relative to the control) at 2.5 ng/ml (150 pM) and occurred exclusively at the early differentiation stage. The response was highly comparable in dose response and timing to effects of HGF treatment, without any additive or synergistic effect from treatment with a combination of both potent upregulators. In contrast, TGF-beta 2 and -beta 3 potently decreased basal Sema3A expression; the maximum effect was at very low concentrations (40 and 8 pM, respectively) and completely cancelled the activities of FGF2 and HGF to upregulate Sema3A. These results therefore encourage the prospect that a time-coordinated increase in HGF, FGF2, and TGF-beta ligands and their receptors promotes a programmed strategy for Sema3A expression that guarantees successful intramuscular motor reinnervation by delaying sprouting and reattachment of motoneuron terminals onto damaged muscle fibers early in regeneration pending restoration of muscle fiber contractile integrity.

DOI： 10.1152/ajpcell.00257.2011

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/j.1740-0929.2009.00734.x

Language：English   Publishing type：Research paper (scientific journal)  

Tatsumi R, Sankoda Y, Anderson JE, Sato Y, Mizunoya W, Shimizu N, Suzuki T, Yamada M, Rhoads RP Jr, Ikeuchi Y, Allen RE. Possible implication of satellite cells in regenerative motoneuritogenesis: HGF upregulates neural chemorepellent Sema3A during myogenic differentiation. Am J Physiol Cell Physiol 297: C238-C252, 2009. First published June 10, 2009; doi: 10.1152/ajpcell.00161.2009.-Regenerative coordination and remodeling of the intramuscular motoneuron network and neuromuscular connections are critical for restoring skeletal muscle function and physiological properties. The regulatory mechanisms of such coordination remain unclear, although both attractive and repulsive axon guidance molecules may be involved in the signaling pathway. Here we show that expression of a neural secreted chemorepellent semaphorin 3A (Sema3A) is remarkably upregulated in satellite cells of resident myogenic stem cells that are positioned beneath the basal lamina of mature muscle fibers, when treated with hepatocyte growth factor (HGF), established as an essential cue in muscle fiber growth and regeneration. When satellite cells were treated with HGF in primary cultures of cells or muscle fibers, Sema3A message and protein were upregulated as revealed by reverse transcription-polymerase chain reaction and immunochemical studies. Other growth factors had no inductive effect except for a slight effect of epidermal growth factor treatment. Sema3A upregulation was HGF dose dependent with a maximum (about 7-to 8-fold units relative to the control) at 10-25 ng/ml and occurred exclusively at the early-differentiation stage, as characterized by the level of myogenin expression and proliferation (bromodeoxyuridine incorporation) of the cells. Neutralizing antibody to the HGF-specific receptor, c-met, did not abolish the HGF response, indicating that c-met may not mediate the Sema3A expression signaling. Finally, in vivo Sema3A was upregulated in the differentiation phase of satellite cells isolated from muscle regenerating following crush injury. Overall, the data highlight a heretofore unexplored and active role for satellite cells as a key source of Sema3A expression triggered by HGF, hence suggesting that regenerative activity toward motor innervation may importantly reside in satellite cells and could be a crucial contributor during postnatal myogenesis.

DOI： 10.1152/ajpcell.00161.2009

Event date： 2023.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：帯広畜産大学 北海道   Country：Japan  

Event date： 2023.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：帯広畜産大学 北海道   Country：Japan  

Event date： 2023.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：帯広畜産大学 北海道   Country：Japan  

Event date： 2023.9

Language：English  

Venue：Portsmouth   Country：United Kingdom  

Event date： 2023.8

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：千里ライフサイエンスセンター, 大阪府   Country：Japan  

Event date： 2023.8

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：千里ライフサイエンスセンター, 大阪府   Country：Japan  

Event date： 2023.5

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：札幌コンベンションセンター   Country：Japan  

Event date： 2023.3

Language：Japanese   Presentation type：Symposium, workshop panel (public)  

Venue：東海大学湘南キャンパス（神奈川県平塚市）   Country：Japan  

Event date： 2023.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東海大学湘南キャンパス（神奈川県平塚市）   Country：Japan  

Event date： 2022.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン開催   Country：Other  

Event date： 2022.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン開催   Country：Other  

Event date： 2022.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン開催   Country：Other  

Event date： 2022.8

Language：English   Presentation type：Symposium, workshop panel (public)  

Venue：オンライン開催   Country：Other  

Event date： 2022.8

Language：English   Presentation type：Oral presentation (general)  

Venue：オンライン開催   Country：Other  

Event date： 2022.3

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2022.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン開催   Country：Japan  

Event date： 2022.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン開催   Country：Japan  

Event date： 2022.3

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：オンライン開催   Country：Japan  

Event date： 2021.12

Language：Japanese  

Venue：京都大学 百周年時計台記念館   Country：Japan  

Event date： 2021.12

Language：Japanese  

Venue：京都大学 百周年時計台記念館   Country：Japan  

Event date： 2021.12

Language：Japanese  

Country：Japan  

Event date： 2021.12

Language：Japanese  

Country：Japan  

Event date： 2021.12

Language：English  

Venue：京都大学 百周年時計台記念館   Country：Japan  

Event date： 2021.12

Language：English  

Country：Japan  

Event date： 2021.10

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Venue：九州大学【オンライン開催】   Country：Japan  

Event date： 2021.9

Language：Japanese  

Country：Japan  

Event date： 2021.9

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東北大学【オンライン開催】   Country：Japan  

Event date： 2021.3

Language：Others  

Country：Other  

Event date： 2021.3

Language：Others  

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Event date： 2021.3

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Event date： 2021.3

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Event date： 2021.3

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Event date： 2021.3

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Event date： 2021.3

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Event date： 2021.3

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Event date： 2021.3

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Event date： 2021.3

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Event date： 2021.3

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Event date： 2021.3

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Event date： 2021.3

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Country：Other  

Event date： 2020.12

Language：Others  

Country：Other  

Event date： 2020.11

Language：Others  

Country：Other  

Event date： 2020.3

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Venue：京都大学 京都府   Country：Other  

Event date： 2020.3

Language：Others  

Venue：京都大学 京都府   Country：Other  

Event date： 2020.3

Language：Others  

Venue：京都大学 京都府   Country：Other  

Event date： 2020.3

Language：Others  

Venue：京都大学 京都府   Country：Other  

Event date： 2020.3

Language：Others  

Venue：京都大学 京都府   Country：Other  

Event date： 2019.12

Language：English  

Venue：Washington, DC   Country：United States  

Netrin-1 synthesized in satellite cells isolated from fast-twitch muscle may promote fast myofiber-type commitment.

Event date： 2019.12

Language：English  

Venue：Washington, DC   Country：United States  

The replacement of myosin molecules in thick filaments is different between slow- and fast-twitch myofibers.

Event date： 2019.10

Language：Others  

Venue：コープイン京都大学生協事業センター 京都府   Country：Other  

Event date： 2019.10

Language：Others  

Venue：コープイン京都大学生協事業センター 京都府   Country：Other  

Event date： 2019.10

Language：Others  

Venue：東北大学 宮城県   Country：Other  

Event date： 2019.10

Language：Others  

Venue：東北大学 宮城県   Country：Other  

Event date： 2019.9

Language：English  

Venue：San José   Country：Costa Rica  

Myogenin has distinct function in satellite cells isolated from different muscles.

Event date： 2019.9

Language：English  

Venue：San José   Country：Costa Rica  

Netrin-1 synthesized in satellite cells may promote fast myofiber-type commitment during myogenic differentiation phase.

Event date： 2019.9

Language：Others  

Venue：岩手大学 岩手県   Country：Other  

Event date： 2019.9

Language：Others  

Venue：佐島マリーナホテル 神奈川県   Country：Other  

Event date： 2019.9

Language：Others  

Venue：佐島マリーナホテル 神奈川県   Country：Other  

Event date： 2019.9

Language：Others  

Venue：佐島マリーナホテル 神奈川県   Country：Other  

Event date： 2019.8

Language：Others  

Venue：東京大学 東京都   Country：Other  

Event date： 2019.8

Language：Others  

Venue：東京大学 東京都   Country：Other  

Event date： 2019.3

Language：Others  

Venue：麻布大学 神奈川県   Country：Other  

Event date： 2019.3

Language：Others  

Venue：麻布大学 神奈川県   Country：Other  

Event date： 2019.3

Language：Others  

Venue：麻布大学 神奈川県   Country：Other  

Event date： 2019.3

Language：Others  

Venue：麻布大学 神奈川県   Country：Other  

Event date： 2019.3

Language：Others  

Venue：麻布大学 神奈川県   Country：Other  

Event date： 2019.3

Language：Others  

Venue：麻布大学 神奈川県   Country：Other  

Event date： 2019.3

Language：Others  

Venue：麻布大学 神奈川県   Country：Other  

Event date： 2018.11

Language：Others  

Venue：大阪大学 大阪府   Country：Other  

Event date： 2018.10

Language：Others  

Venue：機械振興会館 東京都   Country：Other  

Event date： 2018.10

Language：Others  

Venue：機械振興会館 東京都   Country：Other  

Event date： 2018.9

Language：Others  

Venue：つくば国際会議場 茨城県   Country：Other  

Event date： 2018.8

Language：Others  

Venue：川崎医科大学 岡山県   Country：Other  

Event date： 2018.8

Language：Others  

Venue：川崎医科大学 岡山県   Country：Other  

Event date： 2018.8

Language：Others  

Venue：川崎医科大学 岡山県   Country：Other  

Event date： 2018.7

Language：English  

Venue：Steamboat Springs, CO   Country：United States  

Expression profiles of semaphorin and Netrin family subtypes in satellite cells during myogenic differentiation phase: toward further understanding of myofiber-type commitment models.

Event date： 2018.3

Language：Others  

Venue：東京大学 東京都   Country：Other  

Event date： 2018.3

Language：Others  

Venue：東京大学 東京都   Country：Other  

Event date： 2017.9

Language：Others  

Venue：信州大学 長野県   Country：Other  

Event date： 2017.8

Language：Others  

Venue：国立精神・神経医療研究センター 東京都   Country：Other  

Event date： 2017.3

Language：Others  

Venue：神戸大学 兵庫県   Country：Other  

Event date： 2017.3

Language：Others  

Venue：神戸大学 兵庫県   Country：Other  

Event date： 2017.3

Language：Others  

Venue：神戸大学 兵庫県   Country：Other  

Event date： 2017.3

Language：Others  

Venue：神戸大学 兵庫県   Country：Other  

Event date： 2017.3

Language：Others  

Venue：神戸大学 兵庫県   Country：Other  

Event date： 2017.3

Language：Others  

Venue：神戸大学 兵庫県   Country：Other  

Event date： 2017.3

Language：Others  

Venue：神戸大学 兵庫県   Country：Other  

Event date： 2017.3

Language：Others  

Venue：神戸大学 兵庫県   Country：Other  

Event date： 2017.3

Language：Others  

Venue：神戸大学 兵庫県   Country：Other  

Event date： 2016.11

Language：Others  

Venue：ウインクあいち 愛知県   Country：Other  

Event date： 2016.9

Language：Others  

Venue：基礎生物学研究所 愛知県   Country：Other  

Event date： 2016.8

Language：English  

Venue：Kyushu Sangyo University, Fukuoka   Country：Japan  

Satellite cells isolated from soleus muscle may autonomously form slow-twitch fibers via Sema3A signaling.

Event date： 2016.8

Language：Others  

Venue：日本筋学会学術集会 国立精神・神経医療研究センター 東京都   Country：Other  

Event date： 2016.3

Language：Others  

Venue：日本獣医生命科学大学 東京都   Country：Other  

Event date： 2016.3

Language：Others  

Venue：日本獣医生命科学大学 東京都   Country：Other  

Event date： 2016.3

Language：Others  

Venue：日本獣医生命科学大学 東京都   Country：Other  

Event date： 2016.3

Language：Others  

Venue：日本獣医生命科学大学 東京都   Country：Other  

Event date： 2015.12

Language：Others  

Venue：神戸ポートランド 兵庫県   Country：Other  

Event date： 2015.12

Language：Others  

Venue：神戸ポートランド 兵庫県   Country：Other  

Event date： 2015.11

Language：Others  

Venue：九州大学 福岡県   Country：Other  

Event date： 2015.9

Language：Others  

Venue：酪農学園大学 北海道   Country：Other  

Event date： 2015.9

Language：Others  

Venue：酪農学園大学 北海道   Country：Other  

Event date： 2015.8

Language：Others  

Venue：国立精神・神経医療研究センター 東京都   Country：Other  

Event date： 2015.8

Language：Others  

Venue：国立精神・神経医療研究センター 東京都   Country：Other  

Event date： 2015.3

Language：Others  

Venue：宇都宮大学 栃木県   Country：Other  

Event date： 2014.11

Language：Others  

Venue：パシフィコ横浜 神奈川県   Country：Other  

Regulation of skeletal muscle growth by the pro-protein convertase, furin.

Event date： 2014.11

Language：Others  

Venue：パシフィコ横浜 神奈川県   Country：Other  

Sema3A secreted from satellite cells promotes slow-twitch fiber generation.

Event date： 2014.11

Language：English  

Venue：Universitas Gadjah Mada (UGM), Yogyakarta   Country：Indonesia  

Anti-inflammatory macrophages implicate in regenerative moto-neuritogenesis, by promoting myoblast migration and Sema3A expression.

Event date： 2014.10

Language：English  

Venue：Chuncheon, Gangwon   Country：Korea, Republic of  

Functional interaction between Sema3A and Pax7 in proliferation and differentiation of myoblasts.

Event date： 2014.7

Language：English  

Venue：Steamboat Springs, CO   Country：United States  

APOBEC2 deficiency up-regulates myoblast differentiation.

Event date： 2014.7

Language：English  

Venue：Steamboat Springs, CO   Country：United States  

Sema3A secreted from satellite cells promotes slow-twitch fiber generation.

Event date： 2014.7

Language：English  

Venue：Steamboat Springs, CO   Country：United States  

M2 macrophages may implicate in regenerative moto-neuritogenesis, by promoting myoblast migration and Sema3A expression.

Event date： 2014.3

Language：Others  

Venue：つくば国際会議場 茨城県   Country：Other  

Event date： 2014.3

Language：Others  

Venue：つくば国際会議場 茨城県   Country：Other  

Event date： 2013.10

Language：English  

Venue：Cemay-la-Ville   Country：France  

Semaphorin 3A secreted from myogenic stem cells promotes slow-twitch muscle fiber generation.

Event date： 2013.9

Language：Others  

Venue：新潟大学 新潟県   Country：Other  

Event date： 2013.9

Language：Others  

Venue：新潟大学 新潟県   Country：Other  

Event date： 2013.3

Language：Others  

Venue：安田女子大学 広島県   Country：Other  

Event date： 2013.3

Language：Others  

Venue：安田女子大学 広島県   Country：Other  

Event date： 2012.12

Language：Others  

Venue：マリンメッセ福岡 福岡県   Country：Other  

Event date： 2012.8

Language：English  

Venue：Lucca   Country：Italy  

Heparan/chondroitin sulfate chains may mediate HGF/FGF2-induced up-regulation of neural chemorepellent Sema3A in satellite cell cultures.

Event date： 2012.8

Language：English  

Venue：Lucca   Country：Italy  

Semaphorin 3A regulates the early differentiation of satellite cells.

Event date： 2012.8

Language：English  

Venue：Lucca   Country：Italy  

Mechano-Sensing Calcium-Influx Machinery that Instigates Skeletal Muscle Satellite Cell Activation.

Event date： 2012.3

Language：Others  

Venue：名古屋大学 愛知県   Country：Other  

Event date： 2012.3

Language：Others  

Venue：名古屋大学 愛知県   Country：Other  

Event date： 2011.8

Language：Others  

Venue：北里大学 青森県   Country：Other  

Event date： 2011.3

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Venue：東京農業大学 神奈川県 ＊東日本大震災の影響により中止 （要旨受理済み）   Country：Other  

Event date： 2010.8

Language：English  

Venue：Suntec Singapore Convention and Exhibition Center, Singapore   Country：Singapore  

Possible implication of satellite cells in regenerative motoneuritogenesis : HGF and FGF2 up-regulates neural chemorepellent Sema3A expression.

Event date： 2010.7

Language：English  

Venue：Carefree, AZ, U.S.A.   Country：United States  

Possible implication of satellite cells in regenerative motoneuritogenesis : Temporal coordination of HGF/FGF2/TGF-beta may regulate neural chemorepellent Sema3A.

Event date： 2010.3

Language：Others  

Venue：明治大学 東京都   Country：Other  

Event date： 2009.9

Language：Others  

Venue：琉球大学 沖縄   Country：Other  

Event date： 2009.9

Language：Others  

Venue：琉球大学 沖縄   Country：Other  

Event date： 2009.5 - 2009.6

Language：English  

Venue：Alfred Lerner Hall at Columbia University, NY, U.S.A.   Country：United States  

A possible role for satellite cells in regenerative motoneurogenesis: HGF up-regulates neural chemorepellent Sema3A expression.

Language：Japanese  

Language：Japanese  

Language：English  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

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Language：Japanese  

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Language：English  

Language：Japanese  

Language：Japanese  

Language：Japanese  

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Language：Others  

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Language：Others   Publishing type：Internal/External technical report, pre-print, etc.  

Language：Others  

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Language：Others  

Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Language：Others   Publishing type：Internal/External technical report, pre-print, etc.  

Language：Others  

Language：Japanese  

Language：Japanese  

口腔扁平上皮癌におけるDKK3の発現と機能解析

Language：Others  

Language：English  

Functional interaction between Sema3A and Pax7 in proliferation and differentiation of myoblasts
Poster presentation

Language：English  

APOBEC2 deficiency up-regulates myoblast differentiation
Poster Presentation

Language：English  

Sema3A secreted from satellite cells promotes slow-twitch fiber generation
Poster Presentation

Language：English  

M2 macrophages may implicate in regenerative moto-neuritogenesis, by promoting myoblast migration and Sema3A expression
Poster Presentation

Language：Japanese   Publishing type：Internal/External technical report, pre-print, etc.  

Language：English  

Semaphorin 3A secreted from myogenic stem cells promotes slow-twitch muscle fiber generation
Poster Presentation

Language：English  

Heparan/chondroitin sulfate chains may mediate HGF/FGF2-induced up-regulation of neural chemorepellent Sema3A in satellite cell cultures
Poster presentation

Language：English  

Semaphorin 3A regulates the early differentiation of satellite cells
Poster presentation

Language：English  

Mechano-Sensing Calcium-Influx Machinery that Instigates Skeletal Muscle Satellite Cell Activation
Selected for Oral presentation

Language：English  

Possible Implication of Satellite Cells in Regenerative Motoneuritogenesis: HGF and FGF2 Upregulate Neural Chemorepellent Sema3A Expression.
Poster presentation

Language：Others  

Language：English  

A Possible Role for Satellite Cells in Regenerative Motoneurogenesis: HGF Up-Regulates Neural Chemorepellent Sema3A Expression
Poster presentation

Type：Peer review 

Number of peer-reviewed articles in foreign language journals：4

Type：Competition, symposium, etc. 

Type：Peer review 

Number of peer-reviewed articles in foreign language journals：1

Number of peer-reviewed articles in Japanese journals：1

Type：Competition, symposium, etc. 

Type：Competition, symposium, etc. 

Type：Peer review 

Number of peer-reviewed articles in foreign language journals：4

Number of peer-reviewed articles in Japanese journals：1

Type：Peer review 

Number of peer-reviewed articles in foreign language journals：1