Updated on 2024/11/01

Information

 

写真a

 
MON HIROAKI
 
Organization
Faculty of Agriculture Department of Bioresource Sciences Associate Professor
School of Agriculture Department of Bioresource and Bioenvironment（Concurrent）
Graduate School of Bioresource and Bioenvironmental Sciences Department of Bioresource Sciences（Concurrent）
Title
Associate Professor
Contact information
メールアドレス
Tel
0928024578
Profile
1)カイコゲノムDNAの安定化・維持機構に関する研究 ゲノムDNAの安定維持は重要な生命活動の一つであり、昆虫においてもその基本メカニズムは他生物と同様であると考えられています。しかし、昆虫は核ゲノムの倍数化など高度かつ特異的な分化形態上の制約から、細部においては昆虫特有の機構を発達させていると予想され、カイコにおけるDNA損傷修復メカニズムの解析に取り組んできました。 2)昆虫分散型動原体染色体の構造と機能、およびその制御に関する研究 動原体は、細胞分裂期における染色体の均等分配に必須ですが、カイコをはじめとする一部の昆虫では、1染色体に多数の動原体(セントロメア)をもつ特殊な染色体構造(分散型動原体)をもっています。分散型動原体がどのような分子基盤によって担われているのかに興味をもち研究を行なっています。また、遺伝子ネットワークを用いた新規遺伝子探索により、染色体動体、小胞体ストレス応答に関わる昆虫特異的な遺伝子を同定しており、その機能解析にも取り組んでいます。 3)カイコゲノムの機能発現と遺伝子機能解析のための研究ツールの開発 遺伝子の機能解析を行う際、市販されている実験系や他生物で使用されているアッセイ系がうまく機能しないことが多々あり、様々な昆虫細胞で機能する既存システムの改良、および新たな研究ツールの開発を行ってきました。代表的なものとしては、ハイスループットな遺伝子阻害実験のための細胞株作出が挙げられます。この細胞株を用いて、細胞周期、オートファジー、糖鎖、小分子ncRNA、ERストレスなど様々なゲノム機能発現に関わる遺伝子の解析を行ってきました。 ４）カイコ-バキュロウイルスを用いたタンパク質発現系の改良 当研究室では、近年タンパク質生産の宿主として注目されているカイコを利用した研究を行っています。特に、カイコとそれに感染するバキュロウイルスを活用し、産業および研究に有用なタンパク質の生産系の改良に取り組んでいます。具体的には、組換えバキュロウイルスを迅速かつ効率的に作製するための新しい方法の開発、バキュロウイルス感染後の宿主カイコの応答反応の解析を行い、産業界と学術界の双方において貢献することを目指しています。
External link

Research Areas

  • Life Science / Cell biology

  • Environmental Science/Agriculture Science / Insect science

Degree

  • PhD (Agriculture)

Research Interests・Research Keywords

  • Research theme：chromosome

    Keyword：chromosome

    Research period： 2024

  • Research theme：Insect genome

    Keyword：Insect genome

    Research period： 2024

  • Research theme：Kinetochore

    Keyword：Kinetochore

    Research period： 2024

  • Research theme：Bombyx mori

    Keyword：Bombyx mori

    Research period： 2024

  • Research theme：RNAi

    Keyword：RNAi

    Research period： 2024

  • Research theme：Improvement of the Silkworm-Baculovirus Expression System

    Keyword：silkworm, baculovirus

    Research period： 2022.4

  • Research theme：Functional identification of uncharacterized genes in the silkworm

    Keyword：Uncharacterized genes, orphan genes

    Research period： 2019.6

  • Research theme：Analysis of silkworm holocentric chromosomes.

    Keyword：Holocentric chromosome, centromere

    Research period： 2016.4

Awards

  • 日本蚕糸学会進歩賞 (技術賞)

    2007.3   日本蚕糸学会   Screening of high-permissive silkworm strains for efficientrecombinant protein production in Autographa californica nuclearpolyhedrosis virus (AcNPV).

Papers

  • Efficient and Accurate BmNPV Bacmid Editing System by Two-step Golden Gate Assembly Reviewed

    Takeru Ebihara, Misaki Shibuya, Ayaka Yamaguchi, Masato Hino, Jae Man Lee, Takahiro Kusakabe, Hiroaki Mon

    Journal of Virological Methods   330   115029 - 115029   2024.12   （ ISSN:0166-0934 eISSN:1879-0984 ）

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.jviromet.2024.115029

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  • Production of Norovirus VLPs of the Nine Representative Genotypes Widely Distributed in Japan using the Silkworm-Baculovirus Expression Vector System Reviewed

    Yuto Tsurumi, Keisuke Morimoto, Akitsu Masuda, Jae Man Lee, Hiroaki Mon, Takahiro Kusakabe

    Journal of Virological Methods   331   115038 - 115038   2024.10   （ ISSN:0166-0934 ）

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.jviromet.2024.115038

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  • Effective expression and characterization of the receptor binding domains in SARS-CoV-2 Spike proteins from original strain and variants of concern using Bombyx mori nucleopolyhedrovirus in silkworm

    Akira Tsukamoto, Lee Jae Man, Kosuke Oyama, Akitsu Masuda, Hiroaki Mon, Tadashi Ueda, Takahiro Kusakabe

    Protein Expression and Purification   218   106450 - 106450   2024.6   （ ISSN:1046-5928 eISSN:1096-0279 ）

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    Language：Others   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.pep.2024.106450

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  • Comprehensive Transcriptome Analysis in the Testis of the Silkworm, Bombyx mori

    Kohei Kakino, Hiroaki Mon, Takeru Ebihara, Masato Hino, Akitsu Masuda, Jae Man Lee, Takahiro Kusakabe

    Insects   14 ( 8 )   684 - 684   2023.8   （ ISSN:2075-4450 eISSN:2075-4450 ）

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    Language：Others   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    Spermatogenesis is an important process in reproduction and is conserved across species, but in Bombyx mori, it shows peculiarities, such as the maintenance of spermatogonia by apical cells and fertilization by dimorphic spermatozoa. In this study, we attempted to characterize the genes expressed in the testis of B. mori, focusing on aspects of expression patterns and gene function by transcriptome comparisons between different tissues, internal testis regions, and Drosophila melanogaster. The transcriptome analysis of 12 tissues of B. mori, including those of testis, revealed the widespread gene expression of 20,962 genes and 1705 testis-specific genes. A comparative analysis of the stem region (SR) and differentiated regions (DR) of the testis revealed 4554 and 3980 specific-enriched genes, respectively. In addition, comparisons with D. melanogaster testis transcriptome revealed homologs of 1204 SR and 389 DR specific-enriched genes that were similarly expressed in equivalent regions of Drosophila testis. Moreover, gene ontology (GO) enrichment analysis was performed for SR-specific enriched genes and DR-specific enriched genes, and the GO terms of several biological processes were enriched, confirming previous findings. This study advances our understanding of spermatogenesis in B. mori and provides an important basis for future research, filling a knowledge gap between fly and mammalian studies.

    DOI： 10.3390/insects14080684

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  • The biological role of core 1β1-3galactosyltransferase (T-synthase) in mucin-type O-glycosylation in Silkworm, Bombyx mori

    Akihiro Morio, Jae Man Lee, Tsuguru Fujii, Hiroaki Mon, Akitsu Masuda, Kohei Kakino, Jian Xu, Yutaka Banno, Takahiro Kusakabe

    Insect Biochemistry and Molecular Biology   156   103936 - 103936   2023.5   （ ISSN:0965-1748 eISSN:1879-0240 ）

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    Language：Others   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.ibmb.2023.103936

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  • High yield production of norovirus GII.4 virus-like particles using silkworm pupae and evaluation of their protective immunogenicity. International journal

    Akitsu Masuda, Jae Man Lee, Takeshi Miyata, Shintaro Sato, Atsushi Masuda, Masahiro Taniguchi, Ryosuke Fujita, Hiroshi Ushijima, Keisuke Morimoto, Takeru Ebihara, Masato Hino, Kohei Kakino, Hiroaki Mon, Takahiro Kusakabe

    Vaccine   41 ( 3 )   766 - 777   2023.1   （ ISSN:0264-410X eISSN:1873-2518 ）

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    Language：English   Publishing type：Research paper (scientific journal)  

    Noroviruses (NoVs) are one of the major causes of acute viral gastroenteritis in humans. Virus-like particles (VLPs) without genomes that mimic the capsid structure of viruses are promising vaccine candidates for the prevention of NoVs infection. To produce large amounts of recombinant protein, including VLPs, the silkworm-expression vector system (silkworm-BEVS) is an efficient and powerful tool. In this study, we constructed a recombinant baculovirus that expresses VP1 protein, the major structural protein of NoV GII.4. Expression analysis showed that the baculovirus-infected silkworm pupae expressed NoV VP1 protein more efficiently than silkworm larval fat bodies. We obtained about 4.9 mg of purified NoV VP1 protein from only five silkworm pupae. The purified VP1 protein was confirmed by dynamic light scattering and electron microscopy to form VLPs of approximately 40 nm in diameter. Antisera from mice immunized with the antigen blocked NoV VLPs binding to histo-blood group antigens of pig gastric mucin and also blocked NoV infection in intestinal epithelial cells derived from human induced pluripotent stem (iPS) cells. Our findings demonstrated that NoV VLP eliciting protective antibodies could be obtained in milligram quantities from a few silkworm pupae using the silkworm-BEVS.

    DOI： 10.1016/j.vaccine.2022.12.015

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  • Construction of gene co-expression networks in cultured silkworm cells and identification of previously uncharacterized lepidopteran-specific genes required for chromosome dynamics. Reviewed International journal

    Hiroaki Mon, Masanao Sato, Jae Man Lee, Takahiro Kusakabe

    Insect biochemistry and molecular biology   151   103875 - 103875   2022.12   （ ISSN:0965-1748 eISSN:1879-0240 ）

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    Advances in sequencing technology and bioinformatics have accelerated gene discovery and homology-based functional annotation in many species, and numerous targeted gene studies have greatly expanded the understanding of gene functions. Nevertheless, there are still many genes that lack homology with genes in other evolutionary lineages and are left as genes with unknown functions. We constructed a gene co-expression network from the Bombyx mori ovary-derived cell line, BmN4, and attempted to infer the biological roles of uncharacterized genes based on the correlation between the function-known and unknown genes. Within this network, we focused on the co-expression modules involved in chromosome architecture, dynamics, and integrity, and selected the uncharacterized genes for subsequent RNAi-based phenotypic screening. This approach enabled the identification of 5 genes whose knockdown led to abnormalities in chromosome dynamics and spindle morphology in mitosis. One of them was a recently characterized gene, BmCenp-T, which plays a central role in building the kinetochore protein complex on the silkworm holocentric chromosomes. In this study, we suggest a method for constructing the gene co-expression network and selecting candidate genes for small-scale RNAi screening. This approach is complementary to homology-based annotation and may be useful for the analysis of lineage-specific uncharacterized genes such as orphan genes.

    DOI： 10.1016/j.ibmb.2022.103875

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  • Characterization of a Novel Heterochromatin Protein 1 Homolog “HP1c” in the Silkworm, Bombyx mori

    Masato Hino, Tsuneyuki Tatsuke, Akihiro Morio, Hiroaki Mon, Jae Man Lee, Akitsu Masuda, Kohei Kakino, Yoshino Tonooka, Takahiro Kusakabe

    Insects   13 ( 7 )   631 - 631   2022.7   （ ISSN:2075-4450 eISSN:2075-4450 ）

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    Heterochromatin protein 1 plays an important role in chromatin structure and gene expression regulation. Three HP1 genes have been found in Homo sapiens, and five HP1 genes have been reported in Drosophila melanogaster. On the other hand, in Bombyx mori, only two HP1 genes, BmHP1a and BmHP1b, were reported. In this research, we have reported the molecular and functional characterization of a novel Bombyx mori HP1 gene (BmHP1c), which had stronger transcriptional repression activity than BmHP1a. BmHP1a and BmHP1b is reported to form homo- and heterodimers, but in co-immunoprecipitation experiments, no homo- or hetero-dimer formation of BmHP1c with the other silkworm HP1s is detected. The intracellular localization of BmHP1c is not only in the nucleus but also in the cytoplasm like mammalian HP1γ. In contrast to human HP1a and b, all three BmHP1s were localized preferentially in the regions poorly stained with DAPI. Interestingly, the double knockdown of BmHP1a and b, but not BmHP1c with a or b, arrested the cell cycle at the G2/M phase. These results suggest that BmHP1c is not essential for cell progression and plays a different role than BmHP1a and BmHP1b.

    DOI： 10.3390/insects13070631

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  • Silkworm FoxL21 plays important roles as a regulator of ovarian development in both oogenesis and ovariole development. International journal

    Miyu Tanaka, Tsuguru Fujii, Hiroaki Mon, Jae Man Lee, Kohei Kakino, Hisayoshi Fukumori, Takeru Ebihara, Takumi Nagasato, Masato Hino, Yoshino Tonooka, Takato Moriyama, Ryosuke Fujita, Yutaka Banno, Takahiro Kusakabe

    Insect biochemistry and molecular biology   143   103737 - 103737   2022.4   （ ISSN:0965-1748 eISSN:1879-0240 ）

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    The ovary is an important organ in reproduction. In insects, especially lepidopteran insects, the oocytes and reproductive organs develop rapidly during the pupal stage. Despite their drastic morphological changes, the molecular mechanisms of ovary development are not fully understood. In this study, it is found that forkhead box transcription factor L2, member 1 (FoxL21), which is known to be involved in ovarian differentiation and maintenance in vertebrates, is required for the development of the ovary in the silkworm, Bombyx mori. FoxL21 was expressed in the ovary and ovariole during the larval and pupal stage, respectively. In silkworms in which FoxL21 was knocked out by genome editing, multiple ovarian dysfunctions, such as, abnormal egg formation, thinning of the ovariole sheaths, and defective connection of the oviductus geminus with the ovariole were observed. Finally, ovarian transplantation experiments using the knockout silkworms revealed that FoxL21 functions in the ovariole, but not in the oviductus geminus.

    DOI： 10.1016/j.ibmb.2022.103737

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  • Leptin Is an Important Endocrine Player That Directly Activates Gonadotropic Cells in Teleost Fish, Chub Mackerel. International journal

    Hirofumi Ohga, Kosuke Ito, Kohei Kakino, Hiroaki Mon, Takahiro Kusakabe, Jae Man Lee, Michiya Matsuyama

    Cells   10 ( 12 )   2021.12

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    DOI： 10.3390/cells10123505

  • Production of an active Mus musculus IL-3 using updated silkworm-based baculovirus expression vector system

    Ryo Nagai, Takeru Ebihara, Kohei Kakino, Akitsu Masuda, Jian Xu, Kosuke Minamihata, Noriho Kamiya, Tatphon Kongkrongtong, Masahiro Kawahara, Hiroaki Mon, Tsuguru Fujii, Takahiro Kusakabe, Jae Man Lee

    Journal of Asia-Pacific Entomology   2021.8

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    DOI： 10.1016/j.aspen.2021.04.011

  • Stable trimer formation of spike protein from porcine epidemic diarrhea virus improves the efficiency of secretory production in silkworms and induces neutralizing antibodies in mice. International journal

    Akitsu Masuda, Jae Man Lee, Takeshi Miyata, Takeru Ebihara, Kohei Kakino, Masato Hino, Ryosuke Fujita, Hiroaki Mon, Takahiro Kusakabe

    Veterinary research   52 ( 1 )   102 - 102   2021.7

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    DOI： 10.1186/s13567-021-00971-5

  • Production of scFv, Fab, and IgG of CR3022 Antibodies Against SARS-CoV-2 Using Silkworm-Baculovirus Expression System

    Takeru Ebihara, Akitsu Masuda, Daisuke Takahashi, Masato Hino, Hiroaki Mon, Kohei Kakino, Tsuguru Fujii, Ryosuke Fujita, Tadashi Ueda, Jae Man Lee, Takahiro Kusakabe

    Molecular Biotechnology   2021.7

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    DOI： 10.1007/s12033-021-00373-0

  • Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system. Invited Reviewed International journal

    Ryosuke Fujita, Masato Hino, Takeru Ebihara, Takumi Nagasato, Akitsu Masuda, Jae Man Lee, Tsuguru Fujii, Hiroaki Mon, Kohei Kakino, Ryo Nagai, Miyu Tanaka, Yoshino Tonooka, Takato Moriyama, Takahiro Kusakabe

    Biochemical and Biophysical Research Communication   2021.6

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    DOI： https://doi.org/10.1016/J.BBRC.2020.06.020

  • Active human and murine tumor necrosis factor α cytokines produced from silkworm baculovirus expression system

    12 ( 6 )   2021.6

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    The tumor necrosis factor α (TNFα) has been employed as a promising reagent in treating autoimmunity and cancer diseases. To meet the substantial requirement of TNFα proteins, we report in this study that mature types of recombinant human and murine TNFα proteins are successfully expressed in the baculovirus expression system using silkworm larvae as hosts. The biological activities of purified products were verified in culture murine L929 cells, showing better performance over a commercial Escherichia coli-derived murine TNFα. By comparing the activity of purified TNFα with or without the tag removal, it is also concluded that the overall activity of purified TNFα cytokines could be further improved by the complete removal of C-terminal fusion tags. Collectively, our current attempt demonstrates an alternative platform for supplying high-quality TNFα products with excellent activities for further pharmaceutical and clinical trials.

    DOI： 10.3390/insects12060517

  • Quick and Easy Assembly of a One-Step qRT-PCR Kit for COVID-19 Diagnostics Using In-House Enzymes. Reviewed International journal

    Masateru Takahashi, Muhammad Tehseen, Rahul Salunke, Etsuko Takahashi, Sara Mfarrej, Mohamed A. Sobhy, Fatimah S. Alhamlan, Sharif Hala, Gerardo Ramos-Mandujano, Ahmed A. Al-Qahtani, Fadwa S. Alofi, Afrah Alsomali, Anwar M. Hashem, Asim Khogeer, Naif A. M. Almontashiri, Jae Man Lee, Hiroaki Mon, Kosuke Sakashita, Mo Li, Takahiro Kusakabe, Arnab Pain, and Samir M. Hamdan

    ACS Omega   2021.3

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    Language：Japanese  

    DOI： 10.1021/acsomega.0c05635

  • Optimization of SARS-CoV-2 Spike Protein Expression in the Silkworm and Induction of Efficient Protective Immunity by Inoculation With Alum Adjuvants. International journal

    Akitsu Masuda, Jae Man Lee, Takeshi Miyata, Hiroaki Mon, Keita Sato, Kosuke Oyama, Yasuteru Sakurai, Jiro Yasuda, Daisuke Takahashi, Tadashi Ueda, Yuri Kato, Motohiro Nishida, Noriko Karasaki, Kohei Kakino, Takeru Ebihara, Takumi Nagasato, Masato Hino, Ayaka Nakashima, Kengo Suzuki, Yoshino Tonooka, Miyu Tanaka, Takato Moriyama, Hirokazu Nakatake, Ryosuke Fujita, Takahiro Kusakabe

    Frontiers in immunology   12   803647 - 803647   2021.1   （ ISSN:1664-3224 ）

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    DOI： 10.3389/fimmu.2021.803647

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  • Efficient production of recombinant T7 endonuclease I using silkworm-baculovirus expression vector system. Reviewed International journal

    Kohei Kakino, Akitsu Masuda, Masato Hino, Takeru Ebihara, Jian Xu, Hiroaki Mon, Ryosuke Fujita, Tsuguru Fujii, Takahiro Kusakabe, Jae Man Lee

    Journal of Asia-Pacific Entomology   2020.8

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    DOI： 10.1016/j.aspen.2020.05.001

  • Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system Reviewed

    Ryosuke Fujita, Masato Hino, Takeru Ebihara, Takumi Nagasato, Akitsu Masuda, Jae Man Lee, Tsuguru Fujii, Hiroaki Mon, Kohei Kakino, Ryo Nagai, Miyu Tanaka, Yoshino Tonooka, Takato Moriyama, Takahiro Kusakabe

    Biochemical and Biophysical Research Communications   529 ( 2 )   257 - 262   2020.8

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    DOI： 10.1016/j.bbrc.2020.06.020

  • Optimal silkworm larva host for high-level production of Mus musculus IL-4 using a baculovirus expression vector system Reviewed

    Masahiko Kobayashi, Jian Xu, Kohei Kakino, Akitsu Masuda, Masato Hino, Naoki Fujimoto, Kosuke Minamihata, Noriho Kamiya, Hiroaki Mon, Hiroshi Iida, Masateru Takahashi, Takahiro Kusakabe, Jae Man Lee

    Journal of Asia-Pacific Entomology   23 ( 1 )   268 - 273   2020.4

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    DOI： 10.1016/j.aspen.2019.12.014

  • Mild inactivation and inhibition of phenoloxidase in silkworm serum for xeno-free culture of insect cells Reviewed

    Hino Masato, Karasaki Noriko, Tatsuke Tsuneyuki, Morokuma Daisuke, Masuda Akitsu, Mon Hiroaki, Lee Jae Man, Kusakabe Takahiro

    Journal of insect biotechnology and sericology   89 ( 1 )   9 - 16   2020.2

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    Mild inactivation and inhibition of phenoloxidase in silkworm serum for xeno-free culture of insect cells

  • Mild inactivation and inhibition of phenoloxidase in silkworm serum for xeno-free culture of insect cells Reviewed

    Masato Hino, Noriko Karasaki, Tsuneyuki Tatsuke, Daisuke Morokuma, Akitsu Masuda, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe

    Journal of Insect Biotechnology and Sericology   89 ( 1 )   9 - 16   2020.1

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    DOI： 10.11416/jibs.89.1_009

  • Expression and Purification of Vaccinia Virus DNA Topoisomerase IB Produced in the Silkworm–Baculovirus Expression System Reviewed

    61 ( 8 )   622 - 630   2019.8

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    Type IB DNA topoisomerases are enzymes to change the topological state of DNA molecules and are essential in studying replication, transcription, and recombination of nucleic acids in vitro. DNA topoisomerase IB from Vaccinia virus (vTopIB) is a 32 kDa, type I eukaryotic topoisomerase, which relaxed positively and negatively supercoiled DNAs without Mg2+ and ATP. Although vTopIB has been effectively produced in E. coli expression system, no studies remain available to explore an alternative platform to express recombinant vTopIB (rvTopIB) in a higher eukaryote, where the one can expect post-translational modifications that affect the activity of rvTopIB. Here in this study, rvTopIB with N-terminal tags was constructed and expressed in a silkworm–baculovirus expression vector system (silkworm–BEVS). We developed a simple two consecutive chromatography purification to obtain highly pure rvTopIB. The final yield of rvTopIB obtained from a baculovirus-infected silkworm larva was 83.25 μg. We also evaluated the activity and function of rvTopIB by the DNA relaxation activity assays using a negatively supercoiled pUC19 plasmid DNA as a substrate. With carefully assessing optimized conditions for the reaction buffer, we found that divalent ions, Mg2+, Mn2+, Ca2+, as well as ATP stimulate the DNA relaxation activity by rvTopIB. The functional and active form of rvTopIB, together with the yields of the protein we obtained, suggests that silkworm–BEVS would be a potential alternative platform to produce eukaryotic topoisomerases on an industrial scale.

    DOI： 10.1007/s12033-019-00184-4

  • Expression and Purification of Vaccinia Virus DNA Topoisomerase IB Produced in the Silkworm-Baculovirus Expression System. Reviewed

    Xu J, Lee JM, Tatsuke T, Ebihara T, Masuda A, Hino M, Morokuma D, Fujita R, Mon H, Kusakabe T, Takahashi M

    Molecular biotechnology   61 ( 8 )   622 - 630   2019.8

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    Expression and Purification of Vaccinia Virus DNA Topoisomerase IB Produced in the Silkworm-Baculovirus Expression System.

    DOI： 10.1007/s12033-019-00184-4

  • Expression and purification of biologically active human granulocyte-macrophage colony stimulating factor (hGM-CSF) using silkworm-baculovirus expression vector system Reviewed

    Yurie Kinoshita, Jian Xu, Akitsu Masuda, Kosuke Minamihata, Noriho Kamiya, Hiroaki Mon, Ryosuke Fujita, Takahiro Kusakabe, Jae Man Lee

    Protein Expression and Purification   159   69 - 74   2019.7

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    DOI： 10.1016/j.pep.2019.03.010

  • Expression and purification of biologically active human granulocyte-macrophage colony stimulating factor (hGM-CSF) using silkworm-baculovirus expression vector system. Reviewed

    Kinoshita Y, Xu J, Masuda A, Minamihata K, Kamiya N, Mon H, Fujita R, Kusakabe T, Lee JM

    Protein expression and purification   159   69 - 74   2019.7

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    Expression and purification of biologically active human granulocyte-macrophage colony stimulating factor (hGM-CSF) using silkworm-baculovirus expression vector system.

    DOI： 10.1016/j.pep.2019.03.010

  • Expression of the thermostable Moloney murine leukemia virus reverse transcriptase by silkworm-baculovirus expression system Reviewed

    Takumi Yano, Jae Man Lee, Jian Xu, Yoshiki Morifuji, Akitsu Masuda, Masato Hino, Daisuke Morokuma, Ryosuke Fujita, Masateru Takahashi, Takahiro Kusakabe, Hiroaki Mon

    Journal of Asia-Pacific Entomology   22 ( 2 )   453 - 457   2019.6

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    DOI： 10.1016/j.aspen.2019.02.008

  • Expression, purification, and characterization of highly active endo-alpha-N-acetylgalactosaminidases expressed by silkworm-baculovirus expression system Reviewed

    Morio Akihiro, Xu Jian, Masuda Akitsu, Kinoshita Yurie, Hino Masato, Morokuma Daisuke, Goda Hatsumi M, Okino Nozomu, Ito Makoto, Mon Hiroaki, Fujita Ryosuke, Kusakabe Takahiro, Lee Jae Man

    JOURNAL OF ASIA-PACIFIC ENTOMOLOGY   22 ( 2 )   404 - 408   2019.6

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    Expression, purification, and characterization of highly active endo-alpha-N-acetylgalactosaminidases expressed by silkworm-baculovirus expression system

    DOI： 10.1016/j.aspen.2019.01.009

  • Expression of the thermostable Moloney murine leukemia virus reverse transcriptase by silkworm-baculovirus expression system Reviewed

    Yano Takumi, Lee Jae Man, Xu Jian, Morifuji Yoshiki, Masuda Akitsu, Hino Masato, Morokuma Daisuke, Fujita Ryosuke, Takahashi Masateru, Kusakabe Takahiro, Mon Hiroaki

    JOURNAL OF ASIA-PACIFIC ENTOMOLOGY   22 ( 2 )   453 - 457   2019.6

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    Expression of the thermostable Moloney murine leukemia virus reverse transcriptase by silkworm-baculovirus expression system

    DOI： 10.1016/j.aspen.2019.02.008

  • Expression, purification, and characterization of highly active endo-α-N-acetylgalactosaminidases expressed by silkworm-baculovirus expression system Reviewed

    22 ( 2 )   404 - 408   2019.6

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    The O-glycosidase, endo-α-N-acetylgalactosaminidase from Enterococcus faecalis (endoEF) catalyzes the cleavage of core 1 and core 3 type O-linked disaccharides between GalNAc and serine or threonine residues from glycoproteins. The endoEF has broad substrate specificity and thus is extensively utilized for the structural and functional analysis of the O-linked glycans. In this study, we expressed and purified the recombinant endoEF (rEndoEF) by using the silkworm-baculovirus expression vector system (Silkworm-BEVS) and confirmed the deglycosylation activity of rEndoEF targeting reporter glycoproteins, which was equivalent to the commercial O-glycosidase. Thus, our study provides important clues to produce highly active rEndoEF O-glycosidases employing silkworm-BEVS as an alternative.

    DOI： 10.1016/j.aspen.2019.01.009

  • Identification of an insecticidal toxin produced by Enterobacter sp. strain 532 isolated from diseased Bombyx mori silkworms Reviewed

    Mai Morishita, Akitsu Masuda, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe, Kosuke Tashiro, Chisa Yasunaga-Aoki, Kazuhiro Iiyama

    FEMS microbiology letters   366 ( 2 )   2019.1

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    DOI： 10.1093/femsle/fny295

  • Identification of an insecticidal toxin produced by Enterobacter sp. strain 532 isolated from diseased Bombyx mori silkworms. Reviewed

    Morishita M, Masuda A, Mon H, Lee JM, Kusakabe T, Tashiro K, Yasunaga-Aoki C, Iiyama K

    FEMS microbiology letters   366 ( 2 )   2019.1

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    Identification of an insecticidal toxin produced by Enterobacter sp. strain 532 isolated from diseased Bombyx mori silkworms.

    DOI： 10.1093/femsle/fny295

  • Toxin complex is a major virulence determinant of Enterobacter sp. 532 against the silkworm, Bombyx mori Reviewed

    Journal of Insect Biotechnology and Sericology   88 ( 1 )   1_021 - 1_025   2019.1

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    DOI： 10.11416/jibs.88.1_021

  • Expression, Purification, and Characterization of Recombinant Human α1-Antitrypsin Produced Using Silkworm-Baculovirus Expression System. Reviewed

    60 ( 12 )   924 - 934   2018.12

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    DOI： 10.1007/s12033-018-0127-y

  • A functional polypeptide N-acetylgalactosaminyltransferase (PGANT) initiates O-glycosylation in cultured silkworm BmN4 cells Reviewed

    Jian Xu, Akihiro Morio, Daisuke Morokuma, Yudai Nagata, Masato Hino, Akitsu Masuda, Zhiqing Li, Hiroaki Mon, Takahiro Kusakabe, Jae Man Lee

    Applied Microbiology and Biotechnology   102 ( 20 )   8783 - 8797   2018.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-018-9309-6

  • A functional polypeptide N-acetylgalactosaminyltransferase (PGANT) initiates O-glycosylation in cultured silkworm BmN4 cells. Reviewed

    Xu J, Morio A, Morokuma D, Nagata Y, Hino M, Masuda A, Li Z, Mon H, Kusakabe T, Lee JM

    Applied microbiology and biotechnology   102 ( 20 )   8783 - 8797   2018.10

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    A functional polypeptide N-acetylgalactosaminyltransferase (PGANT) initiates O-glycosylation in cultured silkworm BmN4 cells.

    DOI： 10.1007/s00253-018-9309-6

  • Purification and characterization of immunogenic recombinant virus-like particles of porcine circovirus type 2 expressed in silkworm pupae. Reviewed International journal

    Akitsu Masuda, Jae Man Lee, Takeshi Miyata, Tetsuo Sato, Shizuka Hayashi, Masato Hino, Daisuke Morokuma, Noriko Karasaki, Hiroaki Mon, Takahiro Kusakabe

    The Journal of general virology   99 ( 7 )   917 - 926   2018.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1099/jgv.0.001087

  • Production of a biologically active human basic fibroblast growth factor using silkworm-baculovirus expression vector system Reviewed

    Akitsu Masuda, Jian Xu, Kosuke Minamihata, Genki Kagawa, Yusei Hamada, Yoshiki Morifuji, Takumi Yano, Masato Hino, Daisuke Morokuma, Noriko Karasaki, Hiroaki Mon, Noriho Kamiya, Takahiro Kusakabe, Jae Man Lee

    Journal of Asia-Pacific Entomology   21 ( 2 )   716 - 720   2018.6

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    Language：Others   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.aspen.2018.05.002

  • Production of a biologically active human basic fibroblast growth factor using silkworm-baculovirus expression vector system Reviewed

    Akitsu Masuda, Jian Xu, Kosuke Minamihata, Genki Kagawa, Yusei Hamada, Yoshiki Morifuji, Takumi Yano, Masato Hino, Daisuke Morokuma, Noriko Karasaki, Hiroaki Mon, Noriho Kamiya, Takahiro Kusakabe, Jae Man Lee

    Journal of Asia-Pacific Entomology   21 ( 2 )   716 - 720   2018.6

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    DOI： 10.1016/j.aspen.2018.05.002

  • Expression, Purification, and Characterization of Recombinant Human α1-Antitrypsin Produced Using Silkworm–Baculovirus Expression System Reviewed

    2018.1

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    Human α1-antitrypsin (AAT) is the most abundant serine proteinase inhibitor (serpin) in the human plasma. Commercially available AAT for the medications of deficiency of α1-antitrypsin is mainly purified from human plasma. There is a high demand for a stable and low-cost supply of recombinant AAT (rAAT). In this study, the baculovirus expression vector system using silkworm larvae as host was employed and a large amount of highly active AAT was recovered from the silkworm serum (~ 15 mg/10 ml) with high purity. Both the enzymatic activity and stability of purified rAAT were comparable with those of commercial product. Our results provide an alternative method for mass production of the active rAAT in pharmaceutical use.

    DOI： 10.1007/s12033-018-0127-y

  • Purification and characterization of immunogenic recombinant virus-like particles of porcine circovirus type 2 expressed in silkworm pupae Reviewed

    Akitsu Masuda, Jae Man Lee, Takeshi Miyata, Tetsuo Sato, Shizuka Hayashi, Masato Hino, Daisuke Morokuma, Noriko Karasaki, Hiroaki Mon, Takahiro Kusakabe

    Journal of General Virology   99 ( 7 )   917 - 926   2018.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1099/jgv.0.001087

  • Modular surface display for porcine circovirus type 2 virus-like particles mediated by microbial transglutaminase Reviewed

    Akitsu Masuda, Kosuke Minamihata, Masato Hino, Daisuke Morokuma, Noriko Karasaki, Hiroaki Mon, Noriho Kamiya, Takahiro Kusakabe, Jae Man Lee

    Journal of Insect Biotechnology and Sericology   87 ( 2 )   53 - 60   2018.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.11416/jibs.87.2_053

  • Molecular characterization of mitochondrial Zucchini and its relation to nuage-piRNA pathway components in Bombyx mori ovary-derived SmN4 cells Reviewed

    Anandrao Ashok Patil, Tsuneyuki Tatsuke, Hiroaki Mon, Jae Man Lee, Daisuke Morokuma, Masato Hino, Takahiro Kusakabe

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   493 ( 2 )   971 - 978   2017.11

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    DOI： 10.1016/j.bbrc.2017.09.107

  • Molecular characterization of mitochondrial Zucchini and its relation to nuage-piRNA pathway components in Bombyx mori ovary-derived BmN4 cells Reviewed

    Anandrao Ashok Patil, Tsuneyuki Tatsuke, Hiroaki Mon, Jae Man Lee, Daisuke Morokuma, Masato Hino, Takahiro Kusakabe

    Biochemical and Biophysical Research Communications   493 ( 2 )   971 - 978   2017.11

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    DOI： 10.1016/j.bbrc.2017.09.107

  • Lipidation of BmAtg8 is required for autophagic degradation of p62 bodies containing ubiquitinated proteins in the silkworm, Bombyx mori Reviewed

    Ming-Ming Ji, Jae Man Lee, Hiroaki Mon, Kazuhiro Iiyama, Tsuneyuki Tatsuke, Daisuke Morokuma, Masato Hino, Mami Yamashita, Kazuma Hirata, Takahiro Kusakabe

    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY   89   86 - 96   2017.10

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    DOI： 10.1016/j.ibmb.2017.08.006

  • Lipidation of BmAtg8 is required for autophagic degradation of p62 bodies containing ubiquitinated proteins in the silkworm, Bombyx mori Reviewed

    Ming Ming Ji, Jae Man Lee, Hiroaki Mon, Kazuhiro Iiyama, Tsuneyuki Tatsuke, Daisuke Morokuma, Masato Hino, Mami Yamashita, Kazuma Hirata, Takahiro Kusakabe

    Insect Biochemistry and Molecular Biology   89   86 - 96   2017.10

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    DOI： 10.1016/j.ibmb.2017.08.006

  • Characterization of Armitage and Yb containing granules and their relationship to nuage in ovary-derived cultured silkworm cell Reviewed

    Anandrao Ashok Patil, Tsuneyuki Tatsuke, Hiroaki Mon, Jae Man Lee, Daisuke Morokuma, Masato Hino, Takahiro Kusakabe

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   490 ( 2 )   134 - 140   2017.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrc.2017.06.008

  • Characterization of Armitage and Yb containing granules and their relationship to nuage in ovary-derived cultured silkworm cell Reviewed

    Anandrao Ashok Patil, Tsuneyuki Tatsuke, Hiroaki Mon, Jae Man Lee, Daisuke Morokuma, Masato Hino, Takahiro Kusakabe

    Biochemical and Biophysical Research Communications   490 ( 2 )   134 - 140   2017.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrc.2017.06.008

  • Identification and functional analysis of outer kinetochore genes in the holocentric insect Bombyx mori Reviewed

    Hiroaki Mon, Jae Man Lee, Masanao Sato, Takahiro Kusakabe

    Insect Biochemistry and Molecular Biology   86   1 - 8   2017.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ibmb.2017.04.005

  • Identification and functional analysis of outer kinetochore genes in the holocentric insect Bombyx mori Reviewed

    Hiroaki Mon, Jae Man Lee, Masanao Sato, Takahiro Kusakabe

    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY   86   1 - 8   2017.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ibmb.2017.04.005

  • Characterization of Recombinant Thermococcus kodakaraensis (KOD) DNA Polymerases Produced Using Silkworm-Baculovirus Expression Vector System Reviewed

    Mami Yamashita, Jian Xu, Daisuke Morokuma, Kazuma Hirata, Masato Hino, Hiroaki Mon, Masateru Takahashi, Samir M. Hamdan, Kosuke Sakashita, Kazuhiro Iiyama, Yutaka Banno, Takahiro Kusakabe, Jae Man Lee

    MOLECULAR BIOTECHNOLOGY   59 ( 6 )   221 - 233   2017.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s12033-017-0008-9

  • Characterization of Recombinant Thermococcus kodakaraensis (KOD) DNA Polymerases Produced Using Silkworm-Baculovirus Expression Vector System Reviewed

    Mami Yamashita, Jian Xu, Daisuke Morokuma, Kazuma Hirata, Masato Hino, Hiroaki Mon, Masateru Takahashi, Samir M. Hamdan, Kosuke Sakashita, Kazuhiro Iiyama, Yutaka Banno, Takahiro Kusakabe, Jae Man Lee

    Molecular Biotechnology   59 ( 6 )   221 - 233   2017.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s12033-017-0008-9

  • Expression and Characterization of Human β-1, 4-Galactosyltransferase 1 (β4GalT1) Using Silkworm–Baculovirus Expression System Reviewed

    59 ( 4-5 )   151 - 158   2017.5

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    Baculovirus expression vector system (BEVS) is widely known as a mass-production tool to produce functional recombinant glycoproteins except that it may not be always suitable for medical practice due to the differences in the structure of N-linked glycans between insects and mammalian. Currently, various approaches have been reported to alter N-linked glycan structures of glycoproteins derived from insects into terminally sialylated complex-type N-glycans. In the light of those studies, we also proposed in vitro maturation of N-glycan with mass-produced and purified glycosyltransferases by silkworm–BEVS. β-1,4-Galactosyltransferase 1 (β4GalT1) is known as one of type II transmembrane enzymes that transfer galactose in a β-1, 4 linkage to accepter sugars, and a key enzyme for further sialylation of N-glycans. In this study, we developed a large-scale production of recombinant human β4GalT1 (rhβ4GalT1) with N- or C-terminal tags in silkworm–BEVS. We demonstrated that rhβ4GalT1 is N-glycosylated and without mucin-type glycosylation. Interestingly, we found that purified rhβ4GalT1 from silkworm serum presented higher galactosyltransferase activity than that expressed from cultured mammalian cells. We also validated the UDP-galactose transferase activity of produced rhβ4GalT1 proteins by using protein subtracts from silkworm silk gland. Taken together, rhβ4GalT1 from silkworms can become a valuable tool for producing high-quality recombinant glycoproteins with mammalian-like N-glycans.

    DOI： 10.1007/s12033-017-0003-1

  • Expression and Characterization of Human β-1, 4-Galactosyltransferase 1 (β4GalT1) Using Silkworm-Baculovirus Expression System. Reviewed

    59 ( 4-5 )   151 - 158   2017.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s12033-017-0003-1

  • Alkaline protease contributes to pyocyanin production in Pseudomonas aeruginosa Reviewed

    Kazuhiro Iiyama, Eigo Takahashi, Jae Man Lee, Hiroaki Mon, Mai Morishita, Takahiro Kusakabe, Chisa Yasunaga-Aoki

    FEMS MICROBIOLOGY LETTERS   364 ( 7 )   2017.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/femsle/fnx051

  • Alkaline protease contributes to pyocyanin production in Pseudomonas aeruginosa Reviewed

    Kazuhiro Iiyama, Eigo Takahashi, Jae Man Lee, Hiroaki Mon, Mai Morishita, Takahiro Kusakabe, Chisa Yasunaga-Aoki

    FEMS microbiology letters   364 ( 7 )   2017.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/femsle/fnx051

  • A reconsideration of the taxonomic position of two bacterial strains isolated from Flacherie-Diseased silkworms in 1965 Reviewed

    Kazuhiro Iiyama, Mai Morishita, Jae Man Lee, Hiroaki Mon, Takahiro Kusakabe, Kosuke Tashiro, Taiki Akasaka, Chisa Yasunaga-Aoki, Kazuhisa Miyamoto

    Journal of Insect Biotechnology and Sericology   86 ( 2 )   35 - 41   2017.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.11416/jibs.86.2_035

  • rRNA degradation in Bombyx mori and Bombyx mandarina cells infected with heterologous nucleopolyhedroviruses Reviewed

    R. Hamajima, C. Yasunaga-Aoki, M. Iwanaga, S. Imanishi, J. Kobayashi, K. Sasaki, T. Kusakabe, J. M. Lee, H. Mon, M. Kobayashi, M. Ikeda

    Journal of Insect Biotechnology and Sericology   85 ( 3 )   73 - 77   2016.12

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  • Proteasome inhibitor MG132 impairs autophagic flux through compromising formation of autophagosomes in Bombyx cells Reviewed

    Ming Ming Ji, Jae Man Lee, Hiroaki Mon, Jian Xu, Tsuneyuki Tatsuke, Takahiro Kusakabe

    Biochemical and Biophysical Research Communications   479 ( 4 )   690 - 696   2016.10

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    DOI： 10.1016/j.bbrc.2016.09.151

  • Proteasome inhibitor MG132 impairs autophagic flux through compromising formation of autophagosomes in Bombyx cells Reviewed

    Ming -Ming Ji, Jae Man Lee, Hiroaki Mon, Jian Xu, Tsuneyuki Tatsuke, Takahiro Kusakabe

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   479 ( 4 )   690 - 696   2016.10

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    DOI： 10.1016/j.bbrc.2016.09.151

  • Co-expression of silkworm allatostatin-C receptor BNGR-A1 with its cognate G protein subunits enhances the GPCR display on the budding baculovirus Reviewed

    Jianping Chen, Jian Xu, Masato Hino, Mami Yamashita, Kazuma Hirata, Anandrao Ashok Patil, Tsuneyuki Tatsuke, Hiroaki Mon, Yutaka Banno, Takahiro Kusakabe, Jae Man Lee

    Journal of Asia-Pacific Entomology   19 ( 3 )   753 - 760   2016.9

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    DOI： 10.1016/j.aspen.2016.07.007

  • Co-expression of silkworm allatostatin-C receptor BNGR-A1 with its cognate G protein subunits enhances the GPCR display on the budding baculovirus Reviewed

    Jianping Chen, Jian Xu, Masato Hino, Mami Yamashita, Kazuma Hirata, Anandrao Ashok Patil, Tsuneyuki Tatsuke, Hiroaki Mon, Yutaka Banno, Takahiro Kusakabe, Jae Man Lee

    JOURNAL OF ASIA-PACIFIC ENTOMOLOGY   19 ( 3 )   753 - 760   2016.9

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    DOI： 10.1016/j.aspen.2016.07.007

  • Expression and Characterization of Recombinant Serratia liquefaciens Nucleases Produced with Baculovirus-mediated Silkworm Expression System Reviewed

    Kazuhiro Iiyama, Jae Man Lee, Tuneyuki Tatsuke, Hiroaki Mon, Takahiro Kusakabe

    Molecular Biotechnology   58 ( 6 )   393 - 403   2016.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s12033-016-9937-y

  • High-level expression and purification of biologically active human IL-2 using silkworm-baculovirus expression vector system Reviewed

    Masato Hino, Takuji Kawanami, Jian Xu, Daisuke Morokuma, Kazuma Hirata, Mami Yamashita, Noriko Karasaki, Tuneyuki Tatsuke, Hiroaki Mon, Kazuhiro Iiyama, Noriho Kamiya, Yutaka Banno, Takahiro Kusakabe, Jae Man Lee

    JOURNAL OF ASIA-PACIFIC ENTOMOLOGY   19 ( 2 )   313 - 317   2016.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.aspen.2016.03.014

  • Expression and Characterization of Recombinant Serratia liquefaciens Nucleases Produced with Baculovirus-mediated Silkworm Expression System Reviewed

    Kazuhiro Iiyama, Jae Man Lee, Tuneyuki Tatsuke, Hiroaki Mon, Takahiro Kusakabe

    MOLECULAR BIOTECHNOLOGY   58 ( 6 )   393 - 403   2016.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s12033-016-9937-y

  • High-level expression and purification of biologically active human IL-2 using silkworm-baculovirus expression vector system Reviewed

    Masato Hino, Takuji Kawanami, Jian Xu, Daisuke Morokuma, Kazuma Hirata, Mami Yamashita, Noriko Karasaki, Tuneyuki Tatsuke, Hiroaki Mon, Kazuhiro Iiyama, Noriho Kamiya, Yutaka Banno, Takahiro Kusakabe, Jae Man Lee

    Journal of Asia-Pacific Entomology   19 ( 2 )   313 - 317   2016.6

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    DOI： 10.1016/j.aspen.2016.03.014

  • Virulence of lipopolysaccharide-deficient mutants of serratia liquefaciens toward the silkworm, Bombyx mori Reviewed

    Erika Taira, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe, Chisa Yasunaga-Aoki, Kazuhiro Iiyama

    Journal of Insect Biotechnology and Sericology   85 ( 1 )   7 - 14   2016.4

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    DOI： 10.11416/jibs.85.1_007

  • Effect of antibiotics on extracellular protein level in Pseudomonas aeruginosa Reviewed

    Eigo Takahashi, Jae Man Lee, Hiroaki Mon, Yuuka Chieda, Chisa Yasunaga-Aoki, Takahiro Kusakabe, Kazuhiro Iiyama

    PLASMID   84-85   44 - 50   2016.3

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    DOI： 10.1016/j.plasmid.2016.03.001

  • Effect of antibiotics on extracellular protein level in Pseudomonas aeruginosa Reviewed

    Eigo Takahashi, Jae Man Lee, Hiroaki Mon, Yuuka Chieda, Chisa Yasunaga-Aoki, Takahiro Kusakabe, Kazuhiro Iiyama

    Plasmid   84-85   44 - 50   2016.3

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    DOI： 10.1016/j.plasmid.2016.03.001

  • rRNA degradation in Bombyx mori and Bombyx mandarina cells infected witv veterologous nucleopolyvedroviruses Reviewed

    Rina Hamajima, Chisa Yasunaga-Aoki, Masashi Iwanaga, Shigeo Imanishi, Jun Kobayashi, Kuni Sasaki, Takahiro Kusakabe, Jae Man Lee, Hiroaki Mon, Michihiro Kobayashi, Motoko Ikeda

    Journal of Insect Biotechnology and Sericology   85 ( 3 )   73 - 77   2016.1

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  • Characterization of the roles of DNA polymerases, clamp, and clamp loaders during s-phase progression and cell cycle regulation in the silkworm, bombyx mori Reviewed

    Masato Hino, Daisuke Morokuma, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe

    Journal of Insect Biotechnology and Sericology   85 ( 2 )   21 - 29   2016.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.11416/jibs.85.2_021

  • CRISPR/Cas9-mediated knockout of factors in non-homologous end joining pathway enhances gene targeting in silkworm cells Reviewed

    Li Zhu, Hiroaki Mon, Jian Xu, Jae Man Lee, Takahiro Kusakabe

    Scientific reports   5   2015.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/srep18103

  • Comparative proteomic analysis of hemolymph proteins from Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-sensitive or -resistant silkworm strains during infections Reviewed

    Jian Xu, Pingbo Zhang, Takahiro Kusakabe, Hiroaki Mon, Zhiqing Li, Li Zhu, Kazuhiro Iiyama, Yutaka Banno, Daisuke Morokuma, Jae Man Lee

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS   16   36 - 47   2015.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.cbd.2015.07.003

  • CRISPR/Cas9-mediated knockout of factors in non-homologous end joining pathway enhances gene targeting in silkworm cells Reviewed

    Li Zhu, Hiroaki Mon, Jian Xu, Jae Man Lee, Takahiro Kusakabe

    SCIENTIFIC REPORTS   5   18103   2015.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/srep18103

  • Comparative proteomic analysis of hemolymph proteins from Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-sensitive or -resistant silkworm strains during infections Reviewed

    Jian Xu, Pingbo Zhang, Takahiro Kusakabe, Hiroaki Mon, Zhiqing Li, Li Zhu, Kazuhiro Iiyama, Yutaka Banno, Daisuke Morokuma, Jae Man Lee

    Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics   16   36 - 47   2015.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.cbd.2015.07.003

  • Differential N-Glycan modifications of human alpha 1-acid glycoprotein (α1AGP) produced in different silkworm strains using the baculovirus expression system Reviewed

    84 ( 2 )   49 - 53   2015.11

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    N-glycosylation plays an important role in various biological activities and in the structural stability of serum glycoproteins. The baculovirus expression system (BES) is widely used to produce recombinant proteins but in some case it is not suitable for medical use because of the differences in N-linked glycans between insects and mammals. We reported that human serum protein alpha 1-acid protein (α1AGP) is effectively used as a model protein for evaluating the validity of engineering the insect-type N-glycosylation pathway. Using this protein, the productivity and N-linked glycan structures were compared among the 37 different silkworm strains. Interestingly, there was no difference in N-linked glycan structure among the silkworm strains, but there was difference in the degree of N-glycosylation.

    DOI： 10.11416/jibs.84.2_049

  • Roles of silkworm endoplasmic reticulum chaperones in the secretion of recombinant proteins expressed by baculovirus system Reviewed

    Saki Imai, Takahiro Kusakabe, Jian Xu, Zhiqing Li, Shintaro Shirai, Hiroaki Mon, Daisuke Morokuma, Jae Man Lee

    MOLECULAR AND CELLULAR BIOCHEMISTRY   409 ( 1-2 )   255 - 262   2015.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s11010-015-2529-5

  • Roles of silkworm endoplasmic reticulum chaperones in the secretion of recombinant proteins expressed by baculovirus system Reviewed

    Saki Imai, Takahiro Kusakabe, Jian Xu, Zhiqing Li, Shintaro Shirai, Hiroaki Mon, Daisuke Morokuma, Jae Man Lee

    Molecular and cellular biochemistry   409 ( 1-2 )   255 - 262   2015.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s11010-015-2529-5

  • Novel kinetochore protein complex from silkworm holocentric chromosomes Reviewed

    Takahiro Kusakabe, Hiroaki Mon, JaeMan Lee

    PROTEIN SCIENCE   24   288 - 288   2015.10

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  • Logs depends on R2D2 to localize in D2 body-like granules and functions in RNAi pathways in silkworm cells Reviewed

    Li Zhu, Tsuneyuki Tatsuke, Jian Xu, Zhiqing Li, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe

    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY   64   78 - 90   2015.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ibmb.2015.07.011

  • Loqs depends on R2D2 to localize in D2 body-like granules and functions in RNAi pathways in silkworm cells Reviewed

    Li Zhu, Tsuneyuki Tatsuke, Jian Xu, Zhiqing Li, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe

    Insect Biochemistry and Molecular Biology   64   78 - 90   2015.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ibmb.2015.07.011

  • Mass Production of an Active Peptide-N-Glycosidase F Using Silkworm-Baculovirus Expression System Reviewed

    Atsushi Masuda, Jian Xu, Takumi Mitsudome, Yudai Nagata, Daisuke Morokuma, Hiroaki Mon, Yutaka Banno, Takahiro Kusakabe, Jae Man Lee

    Molecular Biotechnology   57 ( 8 )   735 - 745   2015.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s12033-015-9866-1

  • Mass Production of an Active Peptide-N-Glycosidase F Using Silkworm-Baculovirus Expression System Reviewed

    Atsushi Masuda, Jian Xu, Takumi Mitsudome, Yudai Nagata, Daisuke Morokuma, Hiroaki Mon, Yutaka Banno, Takahiro Kusakabe, Jae Man Lee

    MOLECULAR BIOTECHNOLOGY   57 ( 8 )   735 - 745   2015.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s12033-015-9866-1

  • Characterization of KfrA proteins encoded by a plasmid of Paenibacillus popilliae ATCC 14706T Reviewed

    Kazuhiro Iiyama, Hiroaki Mon, Kazuki Mori, Takumi Mitsudome, Jae Man Lee, Takahiro Kusakabe, Kousuke Tashiro, Shin ichiro Asano, Chisa Yasunaga-Aoki

    Meta Gene   4   29 - 44   2015.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.mgene.2015.03.001

  • Improvement of Endo-beta-N-acetylglucosaminidase H production using silkworm-baculovirus protein expression system Reviewed

    Atsushi Masuda, Jian Xu, Takumi Mitsudome, Daisuke Morokuma, Hiroaki Mon, Yutaka Banno, Takahiro Kusakabe, Jae Man Lee

    JOURNAL OF ASIA-PACIFIC ENTOMOLOGY   18 ( 2 )   175 - 180   2015.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.aspen.2015.01.006

  • Human alpha 1-acid glycoprotein as a model protein for glycoanalysis in baculovirus expression vector system Reviewed

    Daisuke Morokuma, Jian Xu, Hiroaki Mon, Kazuma Hirata, Masato Hino, Shoko Kuboe, Mami Yamashita, Takahiro Kusakabe, Jae Man Lee

    JOURNAL OF ASIA-PACIFIC ENTOMOLOGY   18 ( 2 )   303 - 309   2015.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.aspen.2015.03.006

  • Characterization of KfrA proteins encoded by a plasmid of Paenibacillus popilliae ATCC 14706T Reviewed

    Kazuhiro Iiyama, Hiroaki Mon, Kazuki Mori, Takumi Mitsudome, Jae Man Lee, Takahiro Kusakabe, Kousuke Tashiro, Shin-ichiro Asano, Chisa Yasunaga-Aoki

    Meta Gene   4   29 - 44   2015.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.mgene.2015.03.001

  • Human alpha 1-acid glycoprotein as a model protein for glycoanalysis in baculovirus expression vector system Reviewed

    Daisuke Morokuma, Jian Xu, Hiroaki Mon, Kazuma Hirata, Masato Hino, Shoko Kuboe, Mami Yamashita, Takahiro Kusakabe, Jae Man Lee

    Journal of Asia-Pacific Entomology   18 ( 2 )   303 - 309   2015.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.aspen.2015.03.006

  • Biochemical characterization of maintenance DNA methyltransferase DNMT-1 from silkworm, Bombyx mori Reviewed

    Takumi Mitsudome, Hiroaki Mon, Jian Xu, Zhiqing Li, Jae Man Lee, Anandrao Ashok Patil, Atsushi Masuda, Kazuhiro Iiyama, Daisuke Morokuma, Takahiro Kusakabe

    Insect Biochemistry and Molecular Biology   58   55 - 65   2015.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ibmb.2015.01.008

  • Biochemical characterization of maintenance DNA methyltransferase DNMT-1 from silkworm, Bombyx mori Reviewed

    Takumi Mitsudome, Hiroaki Mon, Jian Xu, Zhiqing Li, Jae Man Lee, Anandrao Ashok Patil, Atsushi Masuda, Kazuhiro Iiyama, Daisuke Morokuma, Takahiro Kusakabe

    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY   58   55 - 65   2015.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ibmb.2015.01.008

  • Improvement of Endo-β-N-acetylglucosaminidase H production using silkworm-baculovirus protein expression system Reviewed

    18 ( 2 )   175 - 180   2015.1

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    Endo-β-. N-acetylglucosaminidase H (Endo H) catalyzes cleavage between the GlcNAc residues of the chitobiose core of N-linked glycans, leaving one GlcNAc residues attached to asparagine. Endo H cleaves high mannose and hybrid, but not complex, N-linked oligosaccharides on glycoproteins. Because of its unique specificity, Endo H is widely used for the structural and functional analyses of glycoproteins. In our previous study, the recombinant Endo H was produced as a secreted protein using silkworm-baculovirus expression system, but the yield was low (30. μg Endo H/10. ml larval hemolymph) compared to that of Escherichia coli. In this study, we purified active recombinant Endo H as an intracellular protein from fat body of silkworm infected with the recombinant baculovirus expressing Endo H without the exogenous signal peptide. Remarkably, the yield (9.3. mg from 20 silkworm larvae) was about 310-fold higher than that secreted into larval hemolymph as reported previously. In addition, we screened the silkworm strains maintained in Kyushu University and identified n17 as a high-level expression strain for Endo H.

    DOI： 10.1016/j.aspen.2015.01.006

  • Dynamics of polycomb proteins-mediated histone modifications during UV irradiation-induced DNA damage Reviewed

    Zhiqing Li, Hiroaki Mon, Hitoshi Mitsunobu, Li Zhu, Jian Xu, Jae Man Lee, Takahiro Kusakabe

    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY   55   9 - 18   2014.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ibmb.2014.10.001

  • Dynamics of polycomb proteins-mediated histone modifications during UV irradiation-induced DNA damage Reviewed

    Zhiqing Li, Hiroaki Mon, Hitoshi Mitsunobu, Li Zhu, Jian Xu, Jae Man Lee, Takahiro Kusakabe

    Insect Biochemistry and Molecular Biology   55   9 - 18   2014.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ibmb.2014.10.001

  • Differential contribution of the Fanconi anemia-related proteins to repair of several types of DNA damage in cultured silkworm cells Reviewed

    Ryohei Sugahara, Hiroaki Mon, Jae Man Lee, Takahiro Shiotsuki, Takahiro Kusakabe

    FEBS Letters   588 ( 21 )   3959 - 3963   2014.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.febslet.2014.09.009

  • Differential contribution of the Fanconi anemia-related proteins to repair of several types of DNA damage in cultured silkworm cells Reviewed

    Ryohei Sugahara, Hiroaki Mon, Jae Man Lee, Takahiro Shiotsuki, Takahiro Kusakabe

    FEBS LETTERS   588 ( 21 )   3959 - 3963   2014.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.febslet.2014.09.009

  • A conserved SUMOylation signaling for cell cycle control in a holocentric species Bombyx mori Reviewed

    Zhiqing Li, Hiroaki Mon, Jian Xu, Li Zhu, Jae Man Lee, Takahiro Kusakabe

    Insect Biochemistry and Molecular Biology   51 ( 1 )   71 - 79   2014.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ibmb.2014.05.008

  • A conserved SUMOylation signaling for cell cycle control in a holocentric species Bombyx mori Reviewed

    Zhiqing Li, Hiroaki Mon, Jian Xu, Li Zhu, Jae Man Lee, Takahiro Kusakabe

    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY   51   71 - 79   2014.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ibmb.2014.05.008

  • Phylogenetic relationship of Paenibacillus species based on putative replication origin regions and analysis of an yheCD-like sequence found in this region Reviewed

    Kazuhiro Iiyama, Masahiro Otao, Kazuki Mori, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe, Kousuke Tashiro, Shin-Ichiro Asano, Chisa Yasunaga-Aoki

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   78 ( 5 )   891 - 897   2014.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1080/09168451.2014.905188

  • Draft genome sequence of entomopathogenic Serratia liquefaciens strain FK01 Reviewed

    Erika Taira, Kazuhiro Iiyama, Hiroaki Mon, Kazuki Mori, Taiki Akasaka, Kousuke Tashiro, Chisa Yasunaga-Aoki, Jae Man Lee, Takahiro Kusakabe

    Genome Announcements   2 ( 3 )   2014.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1128/genomeA.00609-14

  • Expression, purification, and characterization of endo-β-N-acetylglucosaminidase H using baculovirus-mediated silkworm protein expression system. Reviewed

    172 ( 8 )   3978 - 3988   2014.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s12010-014-0814-5

  • Characterization and recombinant protein expression of ferritin light chain homologue in the silkworm, Bombyx mori Reviewed

    Sun Mee Hong, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe

    INSECT SCIENCE   21 ( 2 )   135 - 146   2014.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/1744-7917.12031

  • A novel third chromosomal locus controls susceptibility to Autographa californica multiple nucleopolyhedrovirus in the silkworm, Bombyx mori Reviewed

    Jian Xu, Takahiro Kusakabe, Kimiko Yamamoto, Yoshitaka Suetsugu, Hiroaki Mon, Zhiqing Li, Li Zhu, Kazuhiro Iiyama, Yutaka Banno, Kaito Yoshimura, Jae Man Lee

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   98 ( 7 )   3049 - 3058   2014.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-013-5437-1

  • Middle region of FancM interacts with Mhf and Rmi1 in silkworms, a species lacking the Fanconi anaemia (FA) core complex Reviewed

    R. Sugahara, H. Mon, J. M. Lee, T. Kusakabe

    Insect Molecular Biology   23 ( 2 )   185 - 198   2014.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/imb.12072

  • Characterization and recombinant protein expression of ferritin light chain homologue in the silkworm, Bombyx mori Reviewed

    Sun Mee Hong, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe

    Insect Science   21 ( 2 )   135 - 146   2014.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/1744-7917.12031

  • Baculovirus-mediated gene transfer systems in silkworm larvae using constitutive host promoters Reviewed

    Jae Man Lee, Jian Xu, Hiroaki Mon, Takumi Mitsudome, Atsushi Masuda, Kaito Yoshimura, Kazuhiro Iiyama, Yuuka Chieda, Takahiro Kusakabe

    Journal of Asia-Pacific Entomology   17 ( 1 )   73 - 78   2014.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.aspen.2013.10.009

  • Roles of Piwi Proteins in Transcriptional Regulation Mediated by HP1s in Cultured Silkworm Cells Reviewed

    Tsuneyuki Tatsuke, Li Zhu, Zhiqing Li, Hitoshi Mitsunobu, Kaito Yoshimura, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe

    PLOS ONE   9 ( 3 )   e92313   2014.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1371/journal.pone.0092313

  • Establishment of Caenorhabditis elegans SID-1-Dependent DNA Delivery System in Cultured Silkworm Cells Reviewed

    Jian Xu, Kaito Yoshimura, Hiroaki Mon, Zhiqing Li, Li Zhu, Kazuhiro Iiyama, Takahiro Kusakabe, Jae Man Lee

    MOLECULAR BIOTECHNOLOGY   56 ( 3 )   193 - 198   2014.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s12033-013-9694-0

  • Baculovirus-mediated gene transfer systems in silkworm larvae using constitutive host promoters Reviewed

    Jae Man Lee, Jian Xu, Hiroaki Mon, Takumi Mitsudome, Atsushi Masuda, Kaito Yoshimura, Kazuhiro Iiyama, Yuuka Chieda, Takahiro Kusakabe

    JOURNAL OF ASIA-PACIFIC ENTOMOLOGY   17 ( 1 )   73 - 78   2014.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.aspen.2013.10.009

  • Roles of piwi proteins in transcriptional regulation mediated by HP1s in cultured silkworm cells Reviewed

    Tsuneyuki Tatsuke, Li Zhu, Zhiqing Li, Hitoshi Mitsunobu, Kaito Yoshimura, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe

    PloS one   9 ( 3 )   2014.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1371/journal.pone.0092313

  • Establishment of Caenorhabditis elegans SID-1-dependent DNA delivery system in cultured silkworm cells Reviewed

    Jian Xu, Kaito Yoshimura, Hiroaki Mon, Zhiqing Li, Li Zhu, Kazuhiro Iiyama, Takahiro Kusakabe, Jae Man Lee

    Molecular Biotechnology   56 ( 3 )   193 - 198   2014.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s12033-013-9694-0

  • Chromatin-induced spindle assembly plays an important role inmetaphase congression of silkworm holocentric chromosomes Reviewed

    Hiroaki Mon, Jae Man Lee, Kazuei Mita, Marian R. Goldsmith, Takahiro Kusakabe

    Insect Biochemistry and Molecular Biology   45 ( 1 )   40 - 50   2014.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ibmb.2013.11.007

  • Chromatin-induced spindle assembly plays an important role in metaphase congression of silkworm holocentric chromosomes Reviewed

    Hiroaki Mon, Jae Man Lee, Kazuei Mita, Marian R. Goldsmith, Takahiro Kusakabe

    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY   45   40 - 50   2014.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ibmb.2013.11.007

  • A novel third chromosomal locus controls susceptibility to Autographa californica multiple nucleopolyhedrovirus in the silkworm, Bombyx mori Reviewed

    Jian Xu, Takahiro Kusakabe, Kimiko Yamamoto, Yoshitaka Suetsugu, Hiroaki Mon, Zhiqing Li, Li Zhu, Kazuhiro Iiyama, Yutaka Banno, Kaito Yoshimura, Jae Man Lee

    Applied Microbiology and Biotechnology   98 ( 7 )   3049 - 3058   2014.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-013-5437-1

  • Phylogenetic relationship of Paenibacillus species based on putative replication origin regions and analysis of an yheCD-like sequence found in this region Reviewed

    Kazuhiro Iiyama, Masahiro Otao, Kazuki Mori, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe, Kousuke Tashiro, Shin Ichiro Asano, Chisa Yasunaga-Aoki

    Bioscience, Biotechnology and Biochemistry   78 ( 5 )   891 - 897   2014.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1080/09168451.2014.905188

  • Expression, purification, and characterization of endo-β-N- acetylglucosaminidase H using baculovirus-mediated silkworm protein expression system Reviewed

    172 ( 8 )   3978 - 3988   2014.1

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    Endo-β-N-acetylglucosaminidase (Endo H) from Streptomyces plicatus hydrolyzes the core di-GlcNAc units of asparagine-linked oligosaccharides and is regarded as an important tool for glycobiology research. In the present study, we established a large-scale system to produce secreted Endo H using a silkworm-baculovirus expression system (silkworm-BES). The recombinant Endo H purified from silkworm hemolymph had activity comparable to that from recombinant Escherichia coli. As well as its well-characterized substrate RNase B, the Endo H from silkworm-BES was able to deglycosylate the high-mannose glycoproteins from silkworm hemolymph. Interestingly, the secretion amount of recombinant Endo H was significantly varied among the different silkworm strains, which could provide valuable information for larger-scale protein productions from silkworm-BES.

    DOI： 10.1007/s12010-014-0814-5

  • Draft genome sequence of entomopathogenic Serratia liquefaciens strain FK01 Reviewed

    Erika Taira, Kazuhiro Iiyama, Hiroaki Mon, Kazuki Mori, Taiki Akasaka, Kousuke Tashiro, Chisa Yasunaga-Aoki, Jae Man Lee, Takahiro Kusakabe

    Genome Announcements   2 ( 3 )   2014.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1128/genomeA.00609-14

  • Biologically active human bone morphogenetic protein 4 fused to collagen-binding domain produced in silkworm-baculovirus expression system Reviewed

    Saki Imai, Zhiqing Li, Kazuhiro Iiyama, Yoshitaka Miyagawa, Masashi Toyoda, Akihiro Umezawa, Hiroaki Mon, Takahiro Kusakabe, Kaito Yoshimura, Jae Man Lee

    Journal of Insect Biotechnology and Sericology   82 ( 2 )   39 - 44   2013.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.11416/jibs.82.2_039

  • Draft genome sequence of Paenibacillus popilliae ATCC 14706T Reviewed

    Kazuhiro Iiyama, Kazuki Mori, Hiroaki Mon, Yuuka Chieda, Jae Man Lee, Takahiro Kusakabe, Kousuke Tashiro, Shin Ichiro Asano, Chisa Yasunaga-Aoki, Susumu Shimizu

    Journal of Insect Biotechnology and Sericology   82 ( 2 )   45 - 48   2013.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.11416/jibs.82.2_045

  • Draft Genorne Sequence of Paenibacillus popilliae ATCC 14706(T) Reviewed

    liyama Kazuhiro, Mori Kazuki, Mon Hiroaki, Chieda Yuuka, Lee Jae Man, Kusakabe Takahiro, Tashiro Kousuke, Asano Shin-Ichiro, Yasunaga-Aoki Chisa, Shimizu Susumu

    Journal of Insect Biotechnology and Sericology   82 ( 2 )   45 - 48   2013.12

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    Draft Genorne Sequence of Paenibacillus popilliae ATCC 14706(T)

  • Soaking RNAi in Bombyx mori BmN4-SID1 cells arrests cell cycle progression Reviewed

    Hiroaki Mon, Zhiqing Li, Isao Kobayashi, Shuichiro Tomita, JaeMan Lee, Hideki Sezutsu, Toshiki Tamura, Takahiro Kusakabe

    JOURNAL OF INSECT SCIENCE   13   155   2013.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1673/031.013.15501

  • Establishment of a soaking RNA interference and Bombyx mori nucleopolyhedrovirus (BmNPV)-hypersensitive cell line using Bme21 cell Reviewed

    Jian Xu, Hiroaki Mon, Takahiro Kusakabe, Zhiqing Li, Li Zhu, Kazuhiro Iiyama, Atsushi Masuda, Takumi Mitsudome, Jae Man Lee

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   97 ( 24 )   10435 - 10444   2013.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-013-5279-x

  • Soaking RNAi in Bombyx mori BmN4-SID1 cells arrests cell cycle progression Reviewed

    Hiroaki Mon, Zhiqing Li, Isao Kobayashi, Shuichiro Tomita, Jae Man Lee, Hideki Sezutsu, Toshiki Tamura, Takahiro Kusakabe

    Journal of Insect Science   13   2013.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1673/031.013.15501

  • Establishment of a soaking RNA interference and Bombyx mori nucleopolyhedrovirus (BmNPV)-hypersensitive cell line using Bme21 cell Reviewed

    Jian Xu, Hiroaki Mon, Takahiro Kusakabe, Zhiqing Li, Li Zhu, Kazuhiro Iiyama, Atsushi Masuda, Takumi Mitsudome, Jae Man Lee

    Applied Microbiology and Biotechnology   97 ( 24 )   10435 - 10444   2013.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-013-5279-x

  • Characterization of tudor-sn-containing granules in the silkworm, bombyx mori Reviewed

    Li Zhu, Tsuneyuki Tatsuke, Hiroaki Mon, Zhiqing Li, Jian Xu, Jae Man Lee, Takahiro Kusakabe

    Insect Biochemistry and Molecular Biology   43 ( 8 )   664 - 674   2013.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ibmb.2013.04.004

  • Production and characterization of the celery mismatch endonuclease CEL II using baculovirus/silkworm expression system Reviewed

    Hiroaki Mon, JaeMan Lee, Mai Fukushima, Yudai Nagata, Mie Fujii, Jian Xu, Oumi Nishi, Kazuhiro Iiyama, Takahiro Kusakabe

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   97 ( 15 )   6813 - 6822   2013.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-012-4583-1

  • PRODUCTION OF SMALL ANTIBACTERIAL PEPTIDES USING SILKWORM-BACULOVIRUS PROTEIN EXPRESSION SYSTEM Reviewed

    Mai Fukushima, Kazuhiro Iiyama, Jun Yamashita, Masutaka Furue, Gaku Tsuji, Shigeo Imanishi, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe

    PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY   43 ( 6 )   565 - 576   2013.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1080/10826068.2012.762717

  • Characterization of Tudor-sn-containing granules in the silkworm, Bombyx mori Reviewed

    Li Zhu, Tsuneyuki Tatsuke, Hiroaki Mon, Zhiqing Li, Jian Xu, Jae Man Lee, Takahiro Kusakabe

    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY   43 ( 8 )   664 - 674   2013.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ibmb.2013.04.004

  • Production of small antibacterial peptides using silkworm-baculovirus protein expression system Reviewed

    Mai Fukushima, Kazuhiro Iiyama, Jun Yamashita, Masutaka Furue, Gaku Tsuji, Shigeo Imanishi, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe

    Preparative Biochemistry and Biotechnology   43 ( 6 )   565 - 576   2013.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1080/10826068.2012.762717

  • Production and characterization of the celery mismatch endonuclease CEL II using baculovirus/silkworm expression system Reviewed

    Hiroaki Mon, Jaeman Lee, Mai Fukushima, Yudai Nagata, Mie Fujii, Jian Xu, Oumi Nishi, Kazuhiro Iiyama, Takahiro Kusakabe

    Applied Microbiology and Biotechnology   97 ( 15 )   6813 - 6822   2013.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-012-4583-1

  • Phylogenetic analysis of Paenibacillus popilliae and its related taxa based on housekeeping genes Reviewed

    Kazuhiro Iiyama, Oumi Nishi, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe, Shin Ichiro Asano, Chisa Yasunaga-Aoki, Susumu Shimizu

    Journal of Insect Biotechnology and Sericology   82 ( 1 )   1 - 11   2013.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.11416/jibs.82.1_001

  • Genome-wide identification of Argonaute 1- and Argonaute 2-regulating genes revealed an inhibition of macula-like virus by RNAi pathway in the silkworm, Bombyx mori Reviewed

    Li Zhu, Zhiqing Li, Tsuneyuki Tatsuke, Daojun Cheng, Jian Xu, Kaito Yoshimura, Hiroaki Mon, Kazuhiro Iiyama, Jae Man Lee, Qingyou Xia, Takahiro Kusakabe

    Journal of Insect Biotechnology and Sericology   82 ( 1 )   19 - 23   2013.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.11416/jibs.82.1_019

  • RNAi suppression of β-N-acetylglucosaminidase (BmFDL) for complex-type N-linked glycan synthesis in cultured silkworm cells Reviewed

    35 ( 7 )   1009 - 1016   2013.7

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    Glycoproteins have various biological functions including enzymatic activity, protein stability and others. Due to the presence of paucimannosidic N-linked glycans, recombinant proteins from an insect cell expression system may not be suitable for therapeutic use. Because baculovirus expression systems (BESs) are used to produce recombinant proteins, it is of interest to modify the endogenous N-glycosylation pathway in insects to mimic that of mammals. Using a soaking RNAi sensitive cell line, BmN4-SID1, has enabled us to suppress Bombyx mori FDL (BmFDL), an N-linked glycan-specific β-N-acetylglucosaminidase. Western blotting and MALDI-TOF MS demonstrated that the BmFDL depletion almost completely converted the paucimannosidic structures of the recombinant proteins produced by BES into a complex-type structure. This highly efficient, simple and low-cost method can be used for mass production of secretion proteins with complex-type N-linked glycans.

    DOI： 10.1007/s10529-013-1183-9

  • Soaking RNAi-mediated modification of Sf9 cells for baculovirus expression system by ectopic expression of Caenorhabditis elegans SID-1 Reviewed

    Jian Xu, Yudai Nagata, Hiroaki Mon, Zhiqing Li, Li Zhu, Kazuhiro Iiyama, Takahiro Kusakabe, Jae Man Lee

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   97 ( 13 )   5921 - 5931   2013.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-013-4785-1

  • RNAi suppression of β-N-acetylglucosaminidase (BmFDL) for complex-type N-linked glycan synthesis in cultured silkworm cells. Reviewed

    35 ( 7 )   1009 - 1016   2013.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s10529-013-1183-9

  • Soaking RNAi-mediated modification of Sf9 cells for baculovirus expression system by ectopic expression of Caenorhabditis elegans SID-1 Reviewed

    Jian Xu, Yudai Nagata, Hiroaki Mon, Zhiqing Li, Li Zhu, Kazuhiro Iiyama, Takahiro Kusakabe, Jae Man Lee

    Applied Microbiology and Biotechnology   97 ( 13 )   5921 - 5931   2013.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-013-4785-1

  • A MC motif in silkworm Argonaute 1 is indispensible for translation repression Reviewed

    L. Zhu, Y. Masaki, T. Tatsuke, Z. Li, H. Mon, J. Xu, J. M. Lee, T. Kusakabe

    Insect Molecular Biology   22 ( 3 )   320 - 330   2013.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/imb.12023

  • Amino acid deprivation-induced expression of asparagine synthetase regulates the growth and survival of cultured silkworm cells Reviewed

    Zhiqing Li, Li Zhu, Jian Xu, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe

    Archives of Insect Biochemistry and Physiology   83 ( 2 )   57 - 68   2013.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/arch.21091

  • Amino acid deprivation-induced expression of asparagine synthetase regulates the growth and survival of cultured silkworm cells Reviewed

    Zhiqing Li, Li Zhu, Jian Xu, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe

    Archives of insect biochemistry and physiology   83 ( 2 )   57 - 68   2013.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/arch.21091

  • Phylogenetic analysis of Paenibacillus popilliae and its related taxa based on housekeeping genes Reviewed

    Journal of Insect Biotechnology and Sericology   82 ( 1 )   1 - 11   2013.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    Phylogenetic analysis of Paenibacillus popilliae and its related taxa based on housekeeping genes

    DOI： 10.11416/jibs.82.1_001

  • Coexpression of Escherichia coli RNase III in silkworm cells improves the efficiency of RNA interference induced by long hairpin dsRNAs Reviewed

    Jae Man Lee, Yoshito Kojin, Tsuneyuki Tatsuke, Hiroaki Mon, Yoshitaka Miyagawa, Takahiro Kusakabe

    Insect Science   20 ( 1 )   69 - 77   2013.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/j.1744-7917.2012.01569.x

  • Coexpression of Escherichia coli RNase III in silkworm cells improves the efficiency of RNA interference induced by long hairpin dsRNAs Reviewed

    Jae Man Lee, Yoshito Kojin, Tsuneyuki Tatsuke, Hiroaki Mon, Yoshitaka Miyagawa, Takahiro Kusakabe

    Insect Science   20 ( 1 )   69 - 77   2013.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/j.1744-7917.2012.01569.x

  • Cell Cycle-Dependent Recruitment of Polycomb Proteins to the ASNS Promoter Counteracts C/ebp-Mediated Transcriptional Activation in Bombyx mori Reviewed

    Zhiqing Li, Daojun Cheng, Hiroaki Mon, Li Zhu, Jian Xu, Tsuneyuki Tatsuke, Jae Man Lee, Qingyou Xia, Takahiro Kusakabe

    PloS one   8 ( 1 )   2013.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1371/journal.pone.0052320

  • Comparison of signal peptides for efficient protein secretion in the baculovirus-silkworm system Reviewed

    Yasuhiko Soejima, Jae Man Lee, Yudai Nagata, Hiroaki Mon, Kazuhiro Iiyama, Hajime Kitano, Michiya Matsuyama, Takahiro Kusakabe

    CENTRAL EUROPEAN JOURNAL OF BIOLOGY   8 ( 1 )   1 - 7   2013.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.2478/s11535-012-0112-6

  • Cell Cycle-Dependent Recruitment of Polycomb Proteins to the ASNS Promoter Counteracts C/ebp-Mediated Transcriptional Activation in Bombyx mori Reviewed

    Zhiqing Li, Daojun Cheng, Hiroaki Mon, Li Zhu, Jian Xu, Tsuneyuki Tatsuke, Jae Man Lee, Qingyou Xia, Takahiro Kusakabe

    PLOS ONE   8 ( 1 )   e52320   2013.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1371/journal.pone.0052320

  • Comparison of signal peptides for efficient protein secretion in the baculovirus-silkworm system Reviewed

    Yasuhiko Soejima, Jae Man Lee, Yudai Nagata, Hiroaki Mon, Kazuhiro Iiyama, Hajime Kitano, Michiya Matsuyama, Takahiro Kusakabe

    Central European Journal of Biology   8 ( 1 )   1 - 7   2013.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.2478/s11535-012-0112-6

  • Establishment of a Bombyx mori nucleopolyhedrovirus (BmNPV) hyper-sensitive cell line from the silkworm e21 strain Reviewed

    Jae Man Lee, Naoya Kawakami, Hiroaki Mon, Hitoshi Mitsunobu, Kazuhiro Iiyama, Satoshi Ninaki, Katsumi Maenaka, Enoch Y. Park, Takahiro Kusakabe

    BIOTECHNOLOGY LETTERS   34 ( 10 )   1773 - 1779   2012.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s10529-012-0971-y

  • RNA interference induction by long hairpin dsRNAs expressed from chromosomal DNA of Bombyx mori cells Reviewed

    Jae Man Lee, Yoshito Kojin, Tsuneyuki Tatsuke, Hiroaki Mon, Yoshitaka Miyagawa, Takahiro Kusakabe

    Journal of the Faculty of Agriculture, Kyushu University   57 ( 2 )   441 - 445   2012.9

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  • RNA Interference Induction by Long Hairpin dsRNAs Expressed from Chromosomal DNA of Bombyx mori Cells Reviewed

    Jae Man Lee, Yoshito Kojin, Tsuneyuki Tatsuke, Hiroaki Mon, Yoshitaka Miyagawa, Takahiro Kusakabe

    JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY   57 ( 2 )   441 - 445   2012.9

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  • Molecular cloning of BmTUDOR-SN and analysis of its role in the RNAi pathway in the silkworm, Bombyx mori (Lepidoptera Bombycidae) Reviewed

    Li Zhu, Tsuneyuki Tatsuke, Zhiqing Li, Hiroaki Mon, Jian Xu, Jae Man Lee, Takahiro Kusakabe

    Applied Entomology and Zoology   47 ( 3 )   207 - 215   2012.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s13355-012-0109-7

  • Molecular cloning of BmTUDOR-SN and analysis of its role in the RNAi pathway in the silkworm, Bombyx mori (Lepidoptera: Bombycidae) Reviewed

    Li Zhu, Tsuneyuki Tatsuke, Zhiqing Li, Hiroaki Mon, Jian Xu, Jae Man Lee, Takahiro Kusakabe

    APPLIED ENTOMOLOGY AND ZOOLOGY   47 ( 3 )   207 - 215   2012.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s13355-012-0109-7

  • Monoubiquitination-dependent chromatin loading of FancD2 in silkworms, a species lacking the FA core complex Reviewed

    Ryohei Sugahara, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe

    Gene   501 ( 2 )   180 - 187   2012.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.gene.2012.03.071

  • Monoubiquitination-dependent chromatin loading of FancD2 in silkworms, a species lacking the FA core complex Reviewed

    Ryohei Sugahara, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe

    GENE   501 ( 2 )   180 - 187   2012.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.gene.2012.03.071

  • Identification and characterization of polycomb group genes in the silkworm, bombyx mori Reviewed

    Zhiqing Li, Tsuneyuki Tatsuke, Kosuke Sakashita, Li Zhu, Jian Xu, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe

    Molecular biology reports   39 ( 5 )   5575 - 5588   2012.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s11033-011-1362-5

  • Identification and characterization of Polycomb group genes in the silkworm, Bombyx mori Reviewed

    Zhiqing Li, Tsuneyuki Tatsuke, Kosuke Sakashita, Li Zhu, Jian Xu, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe

    MOLECULAR BIOLOGY REPORTS   39 ( 5 )   5575 - 5588   2012.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s11033-011-1362-5

  • Genome-wide identification of polycomb target genes reveals a functional association of pho with scm in bombyx mori Reviewed

    Zhiqing Li, Daojun Cheng, Hiroaki Mon, Tsuneyuki Tatsuke, Li Zhu, Jian Xu, Jae Man Lee, Qingyou Xia, Takahiro Kusakabe

    PloS one   7 ( 4 )   2012.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1371/journal.pone.0034330

  • Genome-Wide Identification of Polycomb Target Genes Reveals a Functional Association of Pho with Scm in Bombyx mori Reviewed

    Zhiqing Li, Daojun Cheng, Hiroaki Mon, Tsuneyuki Tatsuke, Li Zhu, Jian Xu, Jae Man Lee, Qingyou Xia, Takahiro Kusakabe

    PLOS ONE   7 ( 4 )   e34330   2012.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1371/journal.pone.0034330

  • Molecular characterization of heterochromatin proteins 1a and 1b from the silkworm, Bombyx mori Reviewed

    H. Mitsunobu, M. Izumi, H. Mon, T. Tatsuke, J. M. Lee, T. Kusakabe

    Insect Molecular Biology   21 ( 1 )   9 - 20   2012.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/j.1365-2583.2011.01115.x

  • Molecular characterization of heterochromatin proteins 1a and 1b from the silkworm, Bombyx mori. Reviewed

    Mitsunobu H, Izumi M, Mon H, Tatsuke T, Lee JM, Kusakabe T

    Insect molecular biology   21 ( 1 )   9 - 20   2012.2

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    Language：Others  

    Molecular characterization of heterochromatin proteins 1a and 1b from the silkworm, Bombyx mori.

    DOI： 10.1111/j.1365-2583.2011.01115.x

  • Molecular Cloning of the Silkworm p53R2-like Gene Reviewed

    Mie Fujii, Masateru Takahashi, Hiroaki Mon, Tsuneyuki Tatsuke, Jae Man Lee, Takahiro Kusakabe

    JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY   57 ( 1 )   79 - 82   2012.2

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  • Molecular cloning of the silkworm p53r2-like gene Reviewed

    Mie Fujii, Masateru Takahashi, Hiroaki Mon, Tsuneyuki Tatsuke, Jae Man Lee, Takahiro Kusakabe

    Journal of the Faculty of Agriculture, Kyushu University   57 ( 1 )   79 - 82   2012.2

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  • Effective RNA interference in cultured silkworm cells mediated by overexpression of Caenorhabditis elegans SID-1 Reviewed

    Hiroaki Mon, Isao Kobayashi, Shinji Ohkubo, Shuichiro Tomita, Jae Man Lee, Hideki Sezutsu, Toshiki Tamura, Takahiro Kusakabe

    RNA biology   9 ( 1 )   40 - 46   2012.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.4161/rna.9.1.18084

  • Effective RNA interference in cultured silkworm cells mediated by overexpression of Caenorhabditis elegans SID-1 Reviewed

    Hiroaki Mon, Isao Kobayashi, Shinji Ohkubo, Shuichiro Tomita, JaeMan Lee, Hideki Sezutsu, Toshiki Tamura, Takahiro Kusakabe

    RNA BIOLOGY   9 ( 1 )   40 - 46   2012.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.4161/rna.9.1.18084

  • Establishment of a Bombyx mori nucleopolyhedrovirus (BmNPV) hyper-sensitive cell line from the silkworm e21 strain. Reviewed

    Jae Man Lee, Naoya Kawakami, Hiroaki Mon, Hitoshi Mitsunobu, Kazuhiro Iiyama, Satoshi Ninaki, Katsumi Maenaka, Enoch Y. Park, Takahiro Kusakabe

    Biotechnology letters   34 ( 10 )   1773 - 1779   2012.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s10529-012-0971-y

  • Post-translational modifications of the N-terminal tail of histone H3 in holocentric chromosomes of Bombyx mori Reviewed

    Hiroaki Mon, Makiko Izumi, Hitoshi Mitsunobu, Tsuneyuki Tatsuke, Kazuhiro Iiyama, Hiroyuki Jikuya, Jae Man Lee, Takahiro Kusakabe

    Insect Biochemistry and Molecular Biology   41 ( 11 )   902 - 908   2011.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ibmb.2011.08.004

  • Post-translational modifications of the N-terminal tail of histone H3 in holocentric chromosomes of Bombyx mori Reviewed

    Hiroaki Mon, Makiko Izumi, Hitoshi Mitsunobu, Tsuneyuki Tatsuke, Kazuhiro Iiyama, Hiroyuki Jikuya, Jae Man Lee, Takahiro Kusakabe

    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY   41 ( 11 )   902 - 908   2011.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ibmb.2011.08.004

  • Double-strand breaks repair by gene conversion in silkworm holocentric chromosomes Reviewed

    Hiroaki Mon, Jaeman Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    Molecular Genetics and Genomics   286 ( 3-4 )   215 - 224   2011.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00438-011-0640-1

  • Double-strand breaks repair by gene conversion in silkworm holocentric chromosomes Reviewed

    Hiroaki Mon, JaeMan Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    MOLECULAR GENETICS AND GENOMICS   286 ( 3-4 )   215 - 224   2011.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00438-011-0640-1

  • Efficient soluble protein production on transgenic silkworms expressing cytoplasmic chaperones Reviewed

    Sun Mee Hong, Jun Yamashita, Hitoshi Mitsunobu, Keiro Uchino, Isao Kobayashi, Hideki Sezutsu, Toshiki Tamura, Hideki Nakajima, Yoshitaka Miyagawa, Jae Man Lee, Hiroaki Mon, Yoshihiko Miyata, Yutaka Kawaguchi, Takahiro Kusakabe

    Applied Microbiology and Biotechnology   87 ( 6 )   2147 - 2156   2010.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-010-2617-0

  • Efficient soluble protein production on transgenic silkworms expressing cytoplasmic chaperones Reviewed

    Sun Mee Hong, Jun Yamashita, Hitoshi Mitsunobu, Keiro Uchino, Isao Kobayashi, Hideki Sezutsu, Toshiki Tamura, Hideki Nakajima, Yoshitaka Miyagawa, Jae Man Lee, Hiroaki Mon, Yoshihiko Miyata, Yutaka Kawaguchi, Takahiro Kusakabe

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   87 ( 6 )   2147 - 2156   2010.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-010-2617-0

  • Molecular characterization of core histones in the silkworm, Bombyx mori Reviewed

    Hitoshi Mitsunobu, Makiko Izumi, Kazuhiro Iiyama, Hiroyuki Jikuya, Jae Man Lee, Hiroaki Mon, Yutaka Kawaguchi, Takahiro Kusakabe

    Journal of Insect Biotechnology and Sericology   79 ( 3 )   75 - 83   2010

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  • Construction of piggyBac-based vectors using visible and drug-resistance marker for introducing foreign genes into silkworm cultured cells Reviewed

    Tsuneyuki Tatsuke, Sun Mee Hong, Hiromi Tobata, Hiroaki Mon, Jae Man Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    Journal of the Faculty of Agriculture, Kyushu University   54 ( 2 )   397 - 400   2009.10

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  • Construction of piggyBac-based Vectors Using Visible and Drug-resistance Marker for Introducing Foreign Genes into Silkworm Cultured Cells Reviewed

    Tsuneyuki Tatsuke, Sun Mee Hong, Hiromi Tobata, Hiroaki Mon, Jae Man Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY   54 ( 2 )   397 - 400   2009.10

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  • Analysis of protein interactions with two-hybrid system in cultured insect cells Reviewed

    Hiroaki Mon, Ryohei Sugahara, Sun Mee Hong, Jae Man Lee, Yusuke Kamachi, Yutaka Kawaguchi, Takahiro Kusakabe

    Analytical Biochemistry   392 ( 2 )   180 - 182   2009.9

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    DOI： 10.1016/j.ab.2009.05.033

  • Molecular Characterization, Localization, and Distribution of Innexins in the Silkworm, Bombyx mori Reviewed

    Sun Mee Hong, Si Kab Noh, Kyung A. Kim, Hitoshi Mitsunobu, Hiroaki Mon, Jae Man Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    MOLECULAR BIOTECHNOLOGY   43 ( 1 )   52 - 58   2009.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s12033-009-9175-7

  • Analysis of protein interactions with two-hybrid system in cultured insect cells Reviewed

    Hiroaki Mon, Ryohei Sugahara, Sun-Mee Hong, Jae-Man Lee, Yusuke Kamachi, Yutaka Kawaguchi, Takahiro Kusakabe

    ANALYTICAL BIOCHEMISTRY   392 ( 2 )   180 - 182   2009.9

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    Language：English  

    DOI： 10.1016/j.ab.2009.05.033

  • Molecular characterization, localization, and distribution of innexins in the silkworm, bombyx mori Reviewed

    Sun Mee Hong, Si Kab Noh, Kyung A. Kim, Hitoshi Mitsunobu, Hiroaki Mon, Jae Man Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    Molecular Biotechnology   43 ( 1 )   52 - 58   2009.9

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    DOI： 10.1007/s12033-009-9175-7

  • Establishment of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori Reviewed

    Noriko Karasaki, Hiroaki Mon, Masateru Takahashi, Jae Man Lee, Katsumi Koga, Yutaka Kawaguchi, Takahiro Kusakabe

    Biotechnology letters   31 ( 4 )   495 - 500   2009.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s10529-008-9898-8

  • Establishment of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori Reviewed

    Noriko Karasaki, Hiroaki Mon, Masateru Takahashi, Jae Man Lee, Katsumi Koga, Yutaka Kawaguchi, Takahiro Kusakabe

    BIOTECHNOLOGY LETTERS   31 ( 4 )   495 - 500   2009.4

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    DOI： 10.1007/s10529-008-9898-8

  • Efficient protein expression in Bombyx mori larvae of the strain d17 highly sensitive to B. mori nucleopolyhedrovirus Reviewed

    Naoya Kawakami, Jae Man Lee, Hiroaki Mon, Yuji Kubo, Yutaka Banno, Yutaka Kawaguchi, Katsumi Maenaka, Enoch Y. Park, Katsumi Koga, Takahiro Kusakabe

    Molecular Biotechnology   40 ( 2 )   180 - 185   2008.10

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    DOI： 10.1007/s12033-008-9074-3

  • Efficient protein expression in Bombyx mori larvae of the strain d17 highly sensitive to B-Mori nucleopolyhedrovirus Reviewed

    Naoya Kawakami, Jae Man Lee, Hiroaki Mon, Yuji Kubo, Yutaka Banno, Yutaka Kawaguchi, Katsumi Maenaka, Enoch Y. Park, Katsumi Koga, Takahiro Kusakabe

    MOLECULAR BIOTECHNOLOGY   40 ( 2 )   180 - 185   2008.10

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    DOI： 10.1007/s12033-008-9074-3

  • Construction of gene expression systems in insect cell lines using promoters from the silkworm, Bombyx mori Reviewed

    Jae Man Lee, Masateru Takahashi, Hiroaki Mon, Hitoshi Mitsunobu, Katsumi Koga, Yutaka Kawaguchi, Yumiko Nakajima, Takahiro Kusakabe

    Journal of Biotechnology   133 ( 1 )   9 - 17   2008.1

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    DOI： 10.1016/j.jbiotec.2007.08.033

  • カイコ FancJ 遺伝子のクローニング Reviewed

    大久保 慎司, 管原 亮平, 門 宏明, 河口 豊, 日下部 宜宏

    32   71 - 73   2008.1

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  • Construction of gene expression systems in insect cell lines using promoters from the silkworm, Bombyx mori Reviewed

    Jae Man Lee, Masateru Takahashi, Hiroaki Mon, Hitoshi Mitsunobu, Katsumi Koga, Yutaka Kawaguchi, Yumiko Nakajima, Takahiro Kusakabe

    JOURNAL OF BIOTECHNOLOGY   133 ( 1 )   9 - 17   2008.1

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    DOI： 10.1016/j.jbiotec.2007.08.033

  • Heterotrimeric complex of replication protein A, a single-stranded DNA binding protein, from the silkworm, Bombyx mori Reviewed

    Ryohei Sugahara, Hiroaki Mon, Jun Yamashita, Hitoshi Mitsunobu, Jae Man Lee, Yutaka Kawaguchi, Katsumi Koga, Takahiro Kusakabe

    Journal of Insect Biotechnology and Sericology   76 ( 3 )   129 - 135   2007.10

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  • Molecular cloning of silkworm Cdc37 and its interaction with Hsp90 chaperone Reviewed

    Jun Yamashita, Yoshitaka Miyagawa, Ryohei Sugahara, Hiroaki Mon, Hitoshi Mitsunobu, Jae Man Lee, Yutaka Kawaguchi, Takahiro Kusakabe

    Journal of Insect Biotechnology and Sericology   76 ( 3 )   137 - 143   2007.10

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  • Screening of high-permissive silkworm strains for efficient recombinant protein production in Autographa californica nuclear polyhedrosis virus (AcNPV) Reviewed

    Jae Man Lee, Hiroaki Mon, Masateru Takahashi, Naoya Kawakami, Hitoshi Mitsunobu, Yutaka Banno, Katsumi Koga, Keiro Uchino, Yutaka Kawaguchi, Takahiro Kusakabe

    Journal of Insect Biotechnology and Sericology   76 ( 2 )   101 - 105   2007.6

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  • Double-strand break repair by homologous recombination in silkworm cells

    H. Mon, R. Sugahara, J. M. Lee, Y. Kawaguchi, T. Kusakabe

    JOURNAL OF INSECT SCIENCE   7   2007.5

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  • カイコにおけるRPA1遺伝子のクローニング Reviewed

    管原 亮平, 山下 隼, 門 宏明, 河口 豊, 日下部 宜宏

    31   13 - 14   2007.4

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • BmHsp70, BmHsp90 系シャペロンタンパク質の探索とBmHSP40のクローニング Reviewed

    山下 隼, 管原 亮平, 門 宏明, 李 在萬, 河口 豊, 日下部 宜宏

    31   49 - 51   2007.2

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    Language：Others  

  • カイコMnd1とHop2 のクローニング Reviewed

    戸畑 紘実, 門 宏明, 管原 亮平, 吉田 浩子, 李 在萬, 日下部 宜宏, 河口 豊

    31   35 - 36   2007.1

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    Language：Others  

  • Construction of gateway-based destination vectors for detecting subcellular localization of proteins in the silkworm, Bombyx mori Reviewed

    Hitoshi Mitsunobu, Kosuke Sakashita, Hiroaki Mon, Hiroko Yoshida, Jae Man Lee, Yutaka Kawaguchi, Katsumi Koga, Takahiro Kusakabe

    Journal of Insect Biotechnology and Sericology   75 ( 3 )   141 - 145   2006.10

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Radiation resistance and its inheritance in the silkworm, Bombyx mori Reviewed

    Masateru Takahashi, Jae Man Lee, Hiroaki Mon, Hiroko Yoshida, Yutaka Kawaguchi, Hideaki Maekawa, Katsumi Koga, Takahiro Kusakabe

    Journal of the Faculty of Agriculture, Kyushu University   51 ( 2 )   261 - 264   2006.10

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Role of the silkworm argonaute2 homolog gene in double-strand break repair of extrachromosomal DNA Reviewed

    Haruna Tsukioka, Masateru Takahashi, Hiroaki Mon, Kazuhiro Okano, Kazuei Mita, Toru Shimada, Jae Man Lee, Yutaka Kawaguchi, Katsumi Koga, Takahiro Kusakabe

    Nucleic acids research   34 ( 4 )   1092 - 1101   2006.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/nar/gkj507

  • Cell cycle arrest induced by radiation in cultured silkworm cells Reviewed

    Masateru Takahashi, Jae Man Lee, Hiroaki Mon, Yutaka Kawaguchi, Katsumi Koga, Takahiro Kusakabe

    Journal of Insect Biotechnology and Sericology   75 ( 1 )   23 - 30   2006.2

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Role of the silkworm argonaute2 homolog gene in double-strand break repair of extrachromosomal DNA Reviewed

    H Tsukioka, M Takahashi, H Mon, K Okano, K Mita, T Shimada, JM Lee, Y Kawaguchi, K Koga, T Kusakabe

    NUCLEIC ACIDS RESEARCH   34 ( 4 )   1092 - 1101   2006.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/nar/gkj507

  • Radiation resistance and its inheritance in the silkworm, Bombyx mori Reviewed

    Masateru Takahashi, Jae Man Lee, Hiroaki Mon, Hiroko Yoshida, Yutaka Kawaguchi, Hideaki Maekawa, Katsumi Koga, Takahiro Kusakabe

    JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY   51 ( 2 )   261 - 264   2006.2

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Efficient gene transfer into silkworm larval tissues by a combination of sonoporation and lipofection Reviewed

    Jae Man Lee, Masateru Takahashi, Hiroaki Mon, Katsumi Koga, Yutaka Kawaguchi, Takahiro Kusakabe

    Cell Biology International   29 ( 11 )   976 - 979   2005.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.cellbi.2005.07.007

  • Efficient gene transfer into silkworm larval tissues by a combination of sonoporation and lipofection Reviewed

    JM Lee, M Takahashi, H Mon, K Koga, Y Kawaguchi, T Kusakabe

    CELL BIOLOGY INTERNATIONAL   29 ( 11 )   976 - 979   2005.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.cellbi.2005.07.007

  • In vivo DNA double-strand breaks enhance gene targeting in cultured silkworm cells Reviewed

    Hiroaki Mon, Takahiro Kusakabe, Jae Man Lee, Yutaka Kawaguchi, Katsumi Koga

    Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology   139 ( 1 )   99 - 106   2004.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.cbpc.2004.06.013

  • Molecular cloning and characterization of the translationally controlled tumor protein gene in Bombyx mori Reviewed

    JM Lee, T Kusakabe, Y Kawaguchi, Y Miyagawa, M Takahashi, H Mon, SK Nho, K Koga

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY   139 ( 1 )   35 - 43   2004.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.cbpc.2004.06.004

  • In vivo DNA double-strand breaks enhance gene targeting in cultured silkworm cells Reviewed

    H Mon, T Kusakabe, JM Lee, Y Kawaguchi, K Koga

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY   139 ( 1 )   99 - 106   2004.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.cbpc.2004.06.013

  • Molecular cloning and characterization of the translationally controlled tumor protein gene in Bombyx mori Reviewed

    Jae Man Lee, Takahiro Kusakabe, Yutaka Kawaguchi, Yoshitaka Miyagawa, Masateru Takahashi, Hiroaki Mon, Si Kab Nho, Katsumi Koga

    Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology   139 ( 1 )   35 - 43   2004.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.cbpc.2004.06.004

  • Analysis of artificial and spontaneous parthenogenetic development in mosaic mutations and the parthenogenetic strain of Bombyx mori Reviewed

    T Kusakabe, K Kido, K Kita, Y Banno, H Mon, Y Kawaguchi, K Koga

    INVERTEBRATE REPRODUCTION & DEVELOPMENT   45 ( 2 )   101 - 108   2004.7

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Analysis of artificial and spontaneous parthenogenetic development in mosaic mutations and the parthenogenetic strain of bombyx mori Reviewed

    T. Kusakabe, K. Kido, K. Kita, Y. Banno, H. Mon, Y. Kawaguchi, K. Koga

    Invertebrate Reproduction and Development   45 ( 2 )   101 - 108   2004.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1080/07924259.2004.9652579

  • Analysis of extrachromosomal homologous recombination in cultured silkworm cells Reviewed

    Hiroaki Mon, Takahiro Kusakabe, Hisanori Bando, Katsura Kojima, Yutaka Kawaguchi, Katsumi Koga

    Biochemical and Biophysical Research Communications   312 ( 3 )   684 - 690   2003.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrc.2003.10.169

  • Analysis of extrachromosomal homologous recombination in cultured silkworm cells Reviewed

    H Mon, T Kusakabe, H Bando, K Kojima, Y Kawaguchi, K Koga

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   312 ( 3 )   684 - 690   2003.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrc.2003.10.169

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Presentations

  • カイコにおける機能未知遺伝子KWMTBOMO08400の機能解析

    米澤徳隆・柿野耕平・門 宏明・李 在萬・日下部宜宏

    日本蚕糸学会第94回大会  2024.3 

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    Language：Others   Presentation type：Oral presentation (general)  

    Venue：つくば市   Country：Other  

  • 摂動に対するカイコ培養細胞株BmN4‐SID1のトランスクリプトーム変化とその情報を利用したネットワークモデリング

    佐藤昌直, 佐藤昌直, 門宏明, 徐剣, 李在萬, 笠嶋めぐみ, 鈴木誉保, 坪田拓也, 小林悟, 瀬筒秀樹, 日下部宜宏

    日本生化学会大会(Web)  2015.12 

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    Event date： 2021.10

    Language：Japanese  

    Country：Japan  

  • 二本鎖RNAチャネルタンパク質SID‐1を利用したRNA干渉

    小林功, 門宏明, 内野恵郎, 田村俊樹, 瀬筒秀樹, 日下部宜宏, 冨田秀一郎

    日本蚕糸学会大会・蚕糸・昆虫機能学術講演会  2013.3 

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    Event date： 2021.10

    Language：Japanese  

    Country：Japan  

  • カイコ-バキュロウイルス発現系を用いたSARS-CoV-2に対するCR3022抗体の生産

    海老原健, 高橋大輔, 李在萬, 増田亮津, 藤田龍介, 門宏明, 植田正, 日下部宜宏

    日本蚕糸学会  2021.3 

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    Event date： 2021.6

    Language：Japanese  

    Venue：オンライン開催   Country：Japan  

  • カイコ-バキュロウイルス発現系を用いて生産したノロウイルス様粒子の安定性向上の検討

    森本啓介, 増田亮津, 李在萬, 海老原健, 藤田龍介, 門宏明, 日下部宜宏

    日本蚕糸学会  2021.3 

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    Event date： 2021.6

    Language：Japanese  

    Venue：オンライン開催   Country：Japan  

  • 昆虫生産系におけるプロタンパク質転換システムの改変によるTGF-β成熟の効率化

    小山茜音, 李在萬, 増田亮津, 門宏明, 藤田龍介, 日下部宜宏

    日本蚕糸学会  2021.3 

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    Event date： 2021.3

    Language：Japanese  

    Venue：オンライン開催   Country：Japan  

  • カイコ培養細胞を用いた簡便なオートファジー 評価系の構築

    永里 匠・門 宏明・小林 政彦・藤田 龍 介・李 在萬・日下部 宜宏

    日本蚕糸学会第90回蚕糸・昆虫機能利用学術講演会  2020.3 

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    Event date： 2020.7

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

    Venue：信州大学   Country：Japan  

  • ヒトノロウイルス様粒子のカイコにおける効率的生産と新規経口ワクチン開発のための粒子表面へのタンパク質ディスプレイの試み

    増田 亮津, 李 在萬, 南畑 孝介, 神谷 典穂, 藤田 龍介, 門 宏明, 日下部 宜宏

    第42回日本分子生物学会年会  2019.12 

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    Event date： 2020.7

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

    Venue：福岡国際会議場 マリンメッセ福岡   Country：Japan  

  • カイコに存在するつのTOR遺伝子の機能的違い

    小林 政彦, 門 宏明, 李 在萬, 佐藤 昌直, 藤田 龍介, 日下部 宜宏

    第42回日本分子生物学会年会  2019.12 

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    Event date： 2020.7

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

    Venue：福岡国際会議場 マリンメッセ福岡   Country：Japan  

  • カイコにおける精子形成段階の異なる細胞集団の網羅的遺伝子発現解析

    柿野 耕平, 門 宏明, 藤田 龍介, 李 在萬, 日下部 宜宏

    第42回日本分子生物学会年会  2019.12 

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    Event date： 2020.7

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

    Venue：福岡国際会議場 マリンメッセ福岡   Country：Japan  

  • コロナウイルススパイク抗原のカイコにおける好適生産系統の選抜と分泌促進

    増田 亮津・李 在萬・宮田 健・伴野 豊・藤田 龍介・門 宏明・日下部 宜宏

    日本蚕糸学会第90回蚕糸・昆虫機能利用学術講演会  2020.3 

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    Event date： 2020.7

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

    Venue：信州大学   Country：Japan  

  • カイコ-バキュロウイルス発現系を用いた組換えアデノ随伴ウイルスの大量生産法の検討

    海老原 健・李 在萬・宮川 世志幸・ 増田 亮津・藤田 龍介・門 宏明・日下 部 宜宏

    日本蚕糸学会第90回蚕糸・昆虫機能利用学術講演会  2020.3 

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    Event date： 2020.7

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

    Venue：信州大学   Country：Japan  

  • カイコ複製起点認識複合体(ORC)の機能解析

    日野 真人・門 宏明・藤田 龍介・李 在 萬・日下部 宜宏

    日本蚕糸学会第90回蚕糸・昆虫機能利用学術講演会  2020.3 

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    Event date： 2020.7

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

    Venue：信州大学   Country：Japan  

  • カイコ−バキュロウイルス発現系を用いた豚イ ンフルエンザ予防のための遺伝子組換えワクチン作成検討

    森山 敬登・李 在萬・増田 亮津・玉 城 志博・新川 武・藤田 龍介・門 宏明・日下部 宜宏

    日本蚕糸学会第90回蚕糸・昆虫機能利用学術講演会  2020.3 

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    Event date： 2020.7

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

    Venue：信州大学   Country：Japan  

  • カイコ人工染色体作製に向けたカイコ培養細 胞で維持されるカイコ BAC の選抜

    外岡 芳野・藤本 章晃・日野 真人・ 門 宏明・李 在萬・藤田 龍介・安河内 祐 二・佐原 健・日下部 宜宏

    日本蚕糸学会第90回蚕糸・昆虫機能利用学術講演会  2020.3 

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    Event date： 2020.7

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

    Venue：信州大学   Country：Japan  

  • カイコにおける染色体機能制御に関する Fox 遺伝子

    田中 未祐・門 宏明・矢野 匠・小林 政 彦・藤田 龍介・李 在萬・日下部 宜宏

    日本蚕糸学会第90回蚕糸・昆虫機能利用学術講演会  2020.3 

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    Event date： 2020.7

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

    Venue：信州大学   Country：Japan  

  • 生殖巣特異的に細胞質分裂を制御する新規遺伝子の同定および機能解析

    柿野 耕平・門 宏明・藤井 告・佐藤 昌直・藤田 龍介・李 在萬・日下部 宜宏

    日本蚕糸学会第90回蚕糸・昆虫機能利用学術講演会  2020.3 

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    Event date： 2020.7

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

    Venue：信州大学   Country：Japan  

  • カイコ分散型動原体染色体におけるクロマチンループ形成とその役割

    益田篤, 佐藤昌直, 門宏明, LEE Jae Man, 日下部宜宏

    日本分子生物学会年会プログラム・要旨集(Web)  2012.12 

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    Language：Others  

    Country：Japan  

  • 二本鎖RNAチャネルタンパク質SID‐1を利用したRNA干渉

    小林功, 門宏明, 内野恵郎, 田村俊樹, 瀬筒秀樹, 日下部宜宏, 冨田秀一郎

    日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集  2013.3 

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    Language：Japanese  

    Country：Japan  

  • カイコ分散型動原体染色体ループドメイン形成へのCTCF,CP190の関与

    益田篤, 佐藤昌直, 末次克行, 山本公子, 門宏明, 李在萬, 日下部宜宏

    日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集  2013.3 

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    Language：Others  

    Country：Japan  

  • BmDnmt1,BmDnmt2,bMBDの機能阻害細胞におけるDNAのメチル化

    満留卓実, 門宏明, 佐藤昌直, 末次克行, 山本公子, 李志清, 徐剣, 祝力, 李在萬, 日下部宜宏

    日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集  2013.3 

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    Language：Others  

    Country：Japan  

  • カイコDNMT-1(BmDNMT-1)の生化学的特性と遺伝子転写制御における役割

    満留卓実, 門宏明, 佐藤昌直, 末次克行, 山本公子, 李志清, 徐剣, 祝力, LEE Jae M., 日下部宜宏, 益田篤

    日本分子生物学会年会プログラム・要旨集(Web)  2014.12 

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    Language：Others  

    Country：Japan  

  • カイコにおけるTORシグナル経路の解明

    平田一真, 佐藤昌直, 徐剣, 季明明, 祝力, 日野真人, 山下真実, 諸熊大輔, 門宏明, 李在萬, 日下部宜宏

    日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集  2015.3 

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    Language：Others  

    Country：Japan  

  • 摂動に対するカイコ培養細胞株BmN4‐SID1のトランスクリプトーム変化とその情報を利用したネットワークモデリング

    佐藤昌直, 佐藤昌直, 門宏明, 徐剣, 李在萬, 笠嶋めぐみ, 鈴木誉保, 坪田拓也, 小林悟, 瀬筒秀樹, 日下部宜宏

    日本生化学会大会(Web)  2015.12 

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    Language：Japanese  

    Country：Japan  

  • 「顔無し遺伝子」ハンティングパイプラインの構築:配列情報から機能を推定できない遺伝子の機能推定

    佐藤昌直, 門宏明, 日下部宜宏

    日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集  2017.3 

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    Language：Others  

    Country：Japan  

  • カイコにおける核多角体病ウイルスの重感染排除の観察,および原因因子の探索

    諸熊大輔, 門宏明, 佐藤昌直, 李在萬, 伴戸久徳, 日下部宜宏

    日本生化学会大会(Web)  2017.6 

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    Language：Others  

    Country：Japan  

  • カイコ分散型動原体を構成するDNA結合タンパク質の解析

    門宏明, 佐藤昌直, 李在萬, 日下部宜宏

    日本生化学会大会(Web)  2017.12 

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    Language：Others  

    Country：Japan  

  • 機能未知遺伝子ライブラリーから得られたカイコ分散型動原体を構成する新規遺伝子の解析

    門宏明, 佐藤昌直, 藤田龍介, 李在萬, 日下部宜宏

    日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集  2019.3 

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    Language：Others  

    Country：Japan  

  • カイコに存在する2つのTOR遺伝子の機能的違い

    小林政彦, 門宏明, 李在萬, 佐藤昌直, 藤田龍介, 日下部宜宏

    日本分子生物学会年会プログラム・要旨集(Web)  2019.12 

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    Language：Others  

    Country：Japan  

  • カイコ精巣をモデルとした配偶子形成に関与するカオナシ遺伝子の解析

    柿野 耕平, 門 宏明, 佐藤 昌直, 藤井 告, 日野 真人, 李 在萬, 藤田 龍, 介, 日下部 宜宏

    日本蚕糸学会  2022.3 

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    Language：Others  

    Country：Other  

  • カイコ培養細胞の有糸分裂に関わる機能未知遺伝子の解析

    米澤 徳隆, 門 宏明, 佐藤 昌直, 藤田 龍介, 李 在萬, 日下部 宜宏

    日本蚕糸学会  2022.3 

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    Language：Others  

    Country：Other  

  • カイコ培養細胞を用いた小胞体ストレス応答遺伝子の解析

    谷 菜月, 門 宏明, 李 在萬, 藤田 龍介, 大院農, 日下部, 宜宏

    日本蚕糸学会  2022.3 

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    Language：Others  

    Country：Other  

  • 昆虫生産系プロタンパク質転換システムの改変によるTGF-β成熟の効率化

    小山 茜音, 李 在萬, 増田 亮津, 門 宏明, 藤田 龍介, 日下部 宜宏

    日本分子生物学会年会  2021.12 

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    Language：Others  

    Country：Other  

  • カイコ－バキュロウイルス発現系を用いた豚繁殖・呼吸障害症候群ウイルス（ＰＲＲＳＶ）に対する組換えタンパク質ワクチンの開発

    長谷川 真理子, 李 在萬, 増田 亮津, 門 宏明, 藤田 龍介, 日下部 宜宏

    日本分子生物学会年会  2021.12 

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    Language：Others  

    Country：Other  

  • 流行型ヒトノロウイルス様粒子の精製過程における物性の比較

    森本 啓介, 増田 亮津, 李 在萬, 門 宏明, 藤田 龍介, 日下部 宜宏

    日本分子生物学会年会  2021.12 

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    Language：Others  

    Country：Other  

  • 魚類ウイルス性出血性敗血症ウイルスに対するVLPワクチン開発

    川中 美沙, 李 在萬, 増田 亮津, 森山 敬登, 門 宏明, 藤田 龍介, 日下部 宜宏

    日本分子生物学会年会  2021.12 

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    Language：Others  

    Country：Other  

  • カイコ人工染色体作製に向けたカイコBACの編集と評価

    外岡 芳野, 日野 真人, 藤本 章晃, 門 宏明, 李 在萬, 藤田 龍介, 安河内 祐二, 佐原 健, 日下部 宜宏

    日本分子生物学会年会  2021.12 

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    Language：Others  

    Country：Other  

  • カイコ培養細胞を用いた新規小胞体ストレス応答遺伝子の探索と機能解析

    谷 菜月, 門 宏明, 海老原 健, 李 在萬, 藤田 龍介, 日下部 宜宏

    日本分子生物学会年会  2022.12 

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    Language：Others  

    Country：Other  

  • 鱗翅目昆虫の細胞分裂に関わる機能未知遺伝子の解析

    米澤 徳隆, 門 宏明, 佐藤 昌直, 藤井 告, 日野 真人, 藤田 龍介, 李 在萬, 日下部 宜宏

    日本分子生物学会年会  2022.12 

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    Country：Other  

  • カイコ蛹からの流行型ヒトノロウイルス様粒子の高効率精製法の検討

    森本 啓介, 増田 亮津, 李 在萬, 門 宏明, 藤田 龍介, 日下部 宜宏

    日本分子生物学会年会  2022.12 

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    Language：Others  

    Country：Other  

  • カイコ-バキュロウイルス発現系における、プロタンパク質転換システムの改変によるTGF-β成熟の効率化

    小山 茜音, 李 在萬, 増田 亮津, 門 宏明, 藤田 龍介, 日下部 宣宏

    日本分子生物学会年会  2022.12 

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    Language：Others  

    Country：Other  

  • カイコ-バキュロウイルス発現系を用いた豚繁殖・呼吸障害症候群ウイルスに対する高分子化ワクチンの開発

    長谷川 真理子, 李 在萬, 増田 亮津, 門 宏明, 藤田 龍介, 日下部 宜宏

    日本分子生物学会年会  2022.12 

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  • カイコをモデルとした精子形成の研究基盤のためのトランスクリプトーム解析

    柿野 耕平, 門 宏明, 日下部 宜宏

    日本分子生物学会年会  2022.12 

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    Country：Other  

  • カイコ血清からのSARS-CoV-2スパイクタンパク質に対する組換え中和抗体の大量生産と特性評価

    海老原 健, 高橋 大輔, 増田 亮津, 門 宏明, 李 在萬, 植田 正, 日下部 宜宏

    日本分子生物学会年会  2022.12 

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    Country：Other  

  • カイコにおけるLNPの体内動態

    小川 竜矢, 日下部 宜宏, 門 宏明, 松永 直哉, 大戸 茂弘, 平井 剛, 寄立 麻琴

    日本蚕糸学会  2023.3 

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    Country：Other  

  • カイコ-バキュロウイルス発現系による異なる血清型由来のHuNoV-VLPsの生産

    森本 啓介, 増田 亮津, 李 在萬, 門 宏明, 藤田 龍介, 日下部 宜宏

    日本蚕糸学会  2023.3 

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  • カイコ人工染色体作製に向けた染色体単離手法の検討

    足立 朋範, 日野 真人, 門 宏明, 日下部宜宏

    日本蚕糸学会  2023.3 

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  • カイコ幼虫の脂肪体におけるBmNPV感染動態の解明

    中桐 槙也, 門 宏明, 海老原 健, 日 野 真人, 藤田 龍介, 李 在萬, 日下部 宜宏

    日本蚕糸学会  2023.3 

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  • FBS代替品作製を目的としたカイコ血清の哺乳動物細胞への利用

    齋木 雅大, 日野 真人, 藤井 告, 荒木 啓充, 李 在萬, 門 宏明, 日下部 宜宏, 藤田 龍介

    日本蚕糸学会  2023.3 

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    Country：Other  

  • 分散型動原体をもつカイコ染色体 Invited

    門 宏明

    染色体学会 第74回年会  2023.10 

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    Country：Other  

  • バキュロウイルス非必須遺伝子クラスター欠損株の構築とタンパク質発現解析

    山口礼華, 海老原健, 門 宏明, 李 在萬, 藤田龍介, 増田亮津, 日野真人, 渋谷美咲, 日下部宜宏

    日本蚕糸学会第94回大会  2024.3 

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    Country：Other  

  • ヒトノロウイルスVLPを形成するVP1タンパ ク質の不溶性原因の解明と可溶性向上

    鶴見祐土, 増田亮津, 李 在萬, 門 宏明, 藤田龍介, 日野真人, 藤井 告, 日下部宜宏

    日本蚕糸学会第94回大会  2024.3 

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  • 組換えタンパク質生産性向上の目指したランダム突然変異導入によるカイコバキュロ ウイルス変異体の獲得

    渋谷美咲, 海老原健, 門 宏明, 李 在萬, 山口礼華, 増田亮津, 日野真人, 藤田龍介, 日下部宜宏

    日本蚕糸学会第94回大会  2024.3 

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    Country：Other  

  • 難発現タンパク質の生産を目的とした分泌性の異なるモデルタンパク質の比較解析

    海老原健, 谷 菜月, 李 在萬, 門 宏明, 日下部宜宏

    日本蚕糸学会第94回大会  2024.3 

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    Country：Other  

  • カイコにおけるLNP体内動態の調査

    小川 竜矢, 門 宏明, 松永 直哉, 大戸 茂弘, 平井 剛, 寄立 麻琴, 日下部 宜宏

    第46回日本分子生物学会年会  2023.12 

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    Language：Others  

    Country：Other  

  • カイコの脂肪体におけるBmNPV感染動態の解明

    中桐 槙也, 門 宏明, 海老原 健, 李 在萬, 藤田 龍介, 日野 真人, 日下部 宜宏

    第46回日本分子生物学会年会  2023.12 

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    Country：Other  

  • カイコゲノムに干渉しないヒト転写因子の探索とヒトTBX5を用いた転写制御系の移植

    前田 麻亜子, 坪田 拓也, 門 宏明, 増田 亮津, 松田 拓也, 浅野 眞一郎, 伴戸 久徳, 瀬筒 秀樹, 荒川 和晴, 日下部 宜宏, 佐藤 昌直

    第46回日本分子生物学会年会  2023.12 

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    Country：Other  

  • カイコ人工染色体作製に向けた染色体単離手法の検討およびカイコBACの改良

    足立 朋範, 日野 真人, 門 宏明, 日下部 宜宏

    第46回日本分子生物学会年会  2023.12 

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    Country：Other  

  • カイコ培養細胞を用いた新規小胞体ストレス応答遺伝子の探索と機能解析

    谷 菜月, 門 宏明, 海老原 健, 李 在萬, 藤田 龍介, 日下部 宜宏

    第46回日本分子生物学会年会  2023.12 

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    Language：Others  

    Country：Other  

  • 鱗翅目昆虫の細胞分裂に関わる機能未知遺伝子の解析

    米澤 徳隆, 門 宏明, 柿野 耕平, 佐藤 昌直, 藤田 龍介, 李 在萬, 日下部 宜宏

    第46回日本分子生物学会年会  2023.12 

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    Language：Others  

    Country：Other  

  • 鱗翅目昆虫の細胞分裂に関わる機能未知遺伝子の解析

    米澤 徳隆, 門 宏明, 柿野 耕平, 佐藤 昌直, 藤田 龍介, 李 在萬, 日下部 宜宏

    第46回日本分子生物学会年会  2023.12 

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  • 難発現タンパク質の生産を目的とした分泌性の異なるモデルタンパク質の比較解析

    海老原健, 谷 菜月, 李 在萬, 門 宏明, 日下部宜宏

    日本蚕糸学会第94回大会  2024.3 

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  • 組換えタンパク質生産性向上の目指したランダム突然変異導入によるカイコバキュロ ウイルス変異体の獲得

    渋谷美咲, 海老原健, 門 宏明, 李 在萬, 山口礼華, 増田亮津, 日野真人, 藤田龍介, 日下部宜宏

    日本蚕糸学会第94回大会  2024.3 

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  • 分散型動原体をもつカイコ染色体 Invited

    門 宏明

    染色体学会 第74回年会  2023.10 

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  • ヒトノロウイルスVLPを形成するVP1タンパ ク質の不溶性原因の解明と可溶性向上

    鶴見祐土, 増田亮津, 李 在萬, 門 宏明, 藤田龍介, 日野真人, 藤井 告, 日下部宜宏

    日本蚕糸学会第94回大会  2024.3 

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  • バキュロウイルス非必須遺伝子クラスター欠損株の構築とタンパク質発現解析

    山口礼華, 海老原健, 門 宏明, 李 在萬, 藤田龍介, 増田亮津, 日野真人, 渋谷美咲, 日下部宜宏

    日本蚕糸学会第94回大会  2024.3 

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  • カイコ精巣をモデルとした配偶子形成に関与するカオナシ遺伝子の解析

    柿野 耕平, 門 宏明, 佐藤 昌直, 藤井 告, 日野 真人, 李 在萬, 藤田 龍, 介, 日下部 宜宏

    日本蚕糸学会  2022.3 

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    Presentation type：Oral presentation (general)  

    researchmap

  • カイコ培養細胞を用いた新規小胞体ストレス応答遺伝子の探索と機能解析

    谷 菜月, 門 宏明, 海老原 健, 李 在萬, 藤田 龍介, 日下部 宜宏

    第46回日本分子生物学会年会  2023.12 

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  • カイコ培養細胞を用いた小胞体ストレス応答遺伝子の解析

    谷 菜月, 門 宏明, 李 在萬, 藤田 龍介, 大院農, 日下部, 宜宏

    日本蚕糸学会  2022.3 

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  • カイコ培養細胞の有糸分裂に関わる機能未知遺伝子の解析

    米澤 徳隆, 門 宏明, 佐藤 昌直, 藤田 龍介, 李 在萬, 日下部 宜宏

    日本蚕糸学会  2022.3 

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  • カイコ人工染色体作製に向けた染色体単離手法の検討およびカイコBACの改良

    足立 朋範, 日野 真人, 門 宏明, 日下部 宜宏

    第46回日本分子生物学会年会  2023.12 

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  • カイコゲノムに干渉しないヒト転写因子の探索とヒトTBX5を用いた転写制御系の移植

    前田 麻亜子, 坪田 拓也, 門 宏明, 増田 亮津, 松田 拓也, 浅野 眞一郎, 伴戸 久徳, 瀬筒 秀樹, 荒川 和晴, 日下部 宜宏, 佐藤 昌直

    第46回日本分子生物学会年会  2023.12 

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  • カイコの脂肪体におけるBmNPV感染動態の解明

    中桐 槙也, 門 宏明, 海老原 健, 李 在萬, 藤田 龍介, 日野 真人, 日下部 宜宏

    第46回日本分子生物学会年会  2023.12 

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  • カイコにおける機能未知遺伝子KWMTBOMO08400の機能解析

    米澤徳隆, 柿野耕平, 門 宏明, 李 在萬, 日下部宜宏

    日本蚕糸学会第94回大会  2024.3 

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  • カイコにおけるLNP体内動態の調査

    小川 竜矢, 門 宏明, 松永 直哉, 大戸 茂弘, 平井 剛, 寄立 麻琴, 日下部 宜宏

    第46回日本分子生物学会年会  2023.12 

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  • ウイルス感染性の特徴についての機械学習モデルの確立(Establishing a machine learning model for viral infectivity characterizations)

    Abubakar Nura, Hino Masato, Nagata Kosuke, Saiki Masahiro, Kusakabe Takahiro, Lee Jaeman, Fujii Tsuguru, Mon Hiroaki, Fujita Ryosuke

    衛生動物  2023.3  日本衛生動物学会

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    Language：English  

  • カイコ-バキュロウイルス発現系を用いたナノ粒子化CSFワクチンの開発

    金子 大樹, 増田 亮津, 近藤 祐里奈, 和田 香里, 門 宏明, 日下部 宜宏, 李 在萬

    日本生物工学会大会講演要旨集  2024.8  (公社)日本生物工学会

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    Language：Japanese  

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MISC

  • Transplanting the Human TBX5 Regulatory Elements: In Silico Screening and Evaluation of Transcription Factors Independent of Bombyx mori Transcriptional Regulation

    前田麻亜子, 坪田拓也, 門宏明, 増田亮津, 松田拓也, 浅野眞一郎, 伴戸久徳, 瀬筒秀樹, 瀬筒秀樹, 荒川和晴, 荒川和晴, 日下部宜宏, 佐藤昌直, 佐藤昌直

    日本分子生物学会年会プログラム・要旨集(Web)   46th   2023

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  • Transcriptome analysis for basis of research on spermatogenesis in silkworm, Bombyx mori

    柿野耕平, 門宏明, 日下部宜宏

    日本分子生物学会年会プログラム・要旨集(Web)   45th   2022

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  • カイコ精巣をモデルとした配偶子形成に関与するカオナシ遺伝子の解析

    柿野耕平, 門宏明, 佐藤昌直, 藤井告, 日野真人, 李在萬, 藤田龍介, 日下部宜宏

    蚕糸・昆虫機能利用学術講演会・日本蚕糸学会大会講演要旨集   92nd   2022

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  • カイコ分散型動原体の謎

    門 宏明, 日下部宜宏

    蚕糸・昆虫バイオテック   2012.1

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

Industrial property rights

Patent   Number of applications: 2   Number of registrations: 0
Utility model   Number of applications: 0   Number of registrations: 0
Design   Number of applications: 0   Number of registrations: 0
Trademark   Number of applications: 0   Number of registrations: 0

Professional Memberships

  • the Japanese Society of Sericultural Science

  • THE MOLECULAR BIOLOGY SOCIETY OF JAPAN

  • 日本分子生物学会

  • 日本蚕糸学会

  • the Japanese Society of Sericultural Science

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  • 日本蚕糸学会

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  • THE MOLECULAR BIOLOGY SOCIETY OF JAPAN

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Committee Memberships

  • 日本蚕糸学会   代議員   Domestic

    2021.4 - 2026.3   

  • Vice-chairman   Domestic

    2021.4 - 2023.4   

  • 日本蚕糸学会九州支部   副支部長   Domestic

    2021.4 - 2023.4   

  • Steering committee member   Domestic

    2018.11 - 2020.11   

  • 日本蚕糸学会九州支部   広報幹事   Domestic

    2018.11 - 2020.11   

Academic Activities

  • Screening of academic papers

    Role(s)： Peer review

    2023

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    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：1

  • Screening of academic papers

    Role(s)： Peer review

    2022

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    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：2

  • 日本蚕糸学会誌

    2021.4 - Present

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    Type：Academic society, research group, etc. 

  • Screening of academic papers

    Role(s)： Peer review

    2021

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    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：2

  • Screening of academic papers

    Role(s)： Peer review

    2020

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    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：1

Research Projects

  • Construction of artificial chromosomes using silkworm chromosomes with holocentric kinetochores

    Grant number：21K05617  2021 - 2023

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship：Principal investigator  Grant type：Scientific research funding

    CiNii Research

  • Generation of silkworm lacking silk gland suitable for insect factories

    Grant number：21K19121  2021 - 2023

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Challenging Research(Exploratory)

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    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

    CiNii Research

  • 分散型動原体をもつカイコ染色体を利用した人工染色体の基盤構築

    2021 - 2023

    科学研究費補助金 (文部科学省）

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    Authorship：Principal investigator  Grant type：Contract research

  • カイコーバキュロウイルス発現系による新型コロナウイルスに対する安価なワクチン生産技術の開発

    2020

    木下基礎科学研究基金

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    Authorship：Principal investigator  Grant type：Contract research

Class subject

  • 遺伝学Ⅱ

    2024.12 - 2025.2   Winter quarter

  • 科学英語

    2024.10 - 2025.3   Second semester

  • 農学実験第一

    2024.10 - 2025.3   Second semester

  • 化学実験

    2024.10 - 2025.3   Second semester

  • 農学実験第三

    2024.10 - 2025.3   Second semester

  • 農業生物科学演習第二

    2024.10 - 2024.12   Fall quarter

  • 遺伝学Ⅰ

    2024.10 - 2024.12   Fall quarter

  • ゲノムサイエンスとエピジェネティクス

    2024.10 - 2024.12   Fall quarter

  • 農業生物科学演習第一

    2024.10 - 2024.12   Fall quarter

  • 農業生物資源学プロジェクト演習

    2024.4 - 2025.3   Full year

  • 農業生物科学輪講

    2024.4 - 2025.3   Full year

  • 農業生物科学特別研究第一

    2024.4 - 2025.3   Full year

  • 農業生物科学特別研究第二

    2024.4 - 2025.3   Full year

  • 分子生物学

    2024.4 - 2024.9   First semester

  • 農学実験第二

    2024.4 - 2024.9   First semester

  • 分子生物学実験

    2024.4 - 2024.9   First semester

  • 遺伝学Ⅱ

    2023.12 - 2024.2   Winter quarter

  • 農学実験第三

    2023.10 - 2024.3   Second semester

  • 科学英語

    2023.10 - 2024.3   Second semester

  • 農学実験第一

    2023.10 - 2024.3   Second semester

  • 化学実験

    2023.10 - 2024.3   Second semester

  • 遺伝学Ⅰ

    2023.10 - 2023.12   Fall quarter

  • ゲノムサイエンスとエピジェネティクス

    2023.10 - 2023.12   Fall quarter

  • 農業生物科学演習第一

    2023.10 - 2023.12   Fall quarter

  • 農業生物科学演習第二

    2023.10 - 2023.12   Fall quarter

  • 基幹教育セミナー

    2023.6 - 2023.8   Summer quarter

  • 農業生物科学輪講

    2023.4 - 2024.3   Full year

  • 農業生物科学特別研究第一

    2023.4 - 2024.3   Full year

  • 農業生物科学特別研究第二

    2023.4 - 2024.3   Full year

  • 農業生物資源学プロジェクト演習

    2023.4 - 2024.3   Full year

  • 分子生物学

    2023.4 - 2023.9   First semester

  • 農学実験第二

    2023.4 - 2023.9   First semester

  • 分子生物学実験

    2023.4 - 2023.9   First semester

  • 遺伝学Ⅱ

    2022.12 - 2023.2   Winter quarter

  • 農学実験第三

    2022.10 - 2023.3   Second semester

  • 科学英語

    2022.10 - 2023.3   Second semester

  • 農学実験第一

    2022.10 - 2023.3   Second semester

  • 化学実験

    2022.10 - 2023.3   Second semester

  • ゲノムサイエンスとエピジェネティクス

    2022.10 - 2022.12   Fall quarter

  • 農業生物科学演習第二

    2022.10 - 2022.12   Fall quarter

  • 農業生物科学演習第一

    2022.10 - 2022.12   Fall quarter

  • 遺伝学Ⅰ

    2022.10 - 2022.12   Fall quarter

  • 分子生物学実験

    2022.6 - 2022.8   Summer quarter

  • 農業生物科学特別研究第二

    2022.4 - 2023.3   Full year

  • 農業生物科学特別研究第一

    2022.4 - 2023.3   Full year

  • 農業生物資源学プロジェクト演習

    2022.4 - 2023.3   Full year

  • 分子生物学

    2022.4 - 2022.9   First semester

  • 遺伝学Ⅱ

    2021.12 - 2022.2   Winter quarter

  • 農学実験第三

    2021.10 - 2022.3   Second semester

  • 農学実験第一

    2021.10 - 2022.3   Second semester

  • ゲノムサイエンスとエピジェネティクス

    2021.10 - 2021.12   Fall quarter

  • 農業生物科学演習第二

    2021.10 - 2021.12   Fall quarter

  • 農業生物科学演習第一

    2021.10 - 2021.12   Fall quarter

  • 遺伝学Ⅰ

    2021.10 - 2021.12   Fall quarter

  • 基幹教育セミナー

    2021.6 - 2021.8   Summer quarter

  • 分子生物学実験

    2021.6 - 2021.8   Summer quarter

  • 農業生物科学特別研究第二

    2021.4 - 2022.3   Full year

  • 農業生物科学特別研究第一

    2021.4 - 2022.3   Full year

  • 農業生物資源学プロジェクト演習

    2021.4 - 2022.3   Full year

  • 遺伝学

    2020.10 - 2021.3   Second semester

  • 農学実験第三

    2020.10 - 2021.3   Second semester

  • 農学実験第一

    2020.10 - 2021.3   Second semester

  • ゲノムサイエンスとエピジェネティクス

    2020.10 - 2020.12   Fall quarter

  • 農業生物科学演習第二

    2020.10 - 2020.12   Fall quarter

  • 農業生物科学演習第一

    2020.10 - 2020.12   Fall quarter

  • 分子生物学実験

    2020.6 - 2020.8   Summer quarter

  • 農業生物科学特別研究第二

    2020.4 - 2021.3   Full year

  • 農業生物科学特別研究第一

    2020.4 - 2021.3   Full year

  • 農業生物資源学プロジェクト演習

    2020.4 - 2021.3   Full year

  • 遺伝学

    2019.10 - 2020.3   Second semester

  • 農学実験第三

    2019.10 - 2020.3   Second semester

  • 農学実験第一

    2019.10 - 2020.3   Second semester

  • ゲノムサイエンスとエピジェネティクス

    2019.10 - 2019.12   Fall quarter

  • 農業生物科学演習第二

    2019.10 - 2019.12   Fall quarter

  • 農業生物科学演習第一

    2019.10 - 2019.12   Fall quarter

  • 農業生物資源学プロジェクト演習

    2019.4 - 2020.3   Full year

  • 農業生物科学特別研究第二

    2019.4 - 2020.3   Full year

  • 農業生物科学特別研究第一

    2019.4 - 2020.3   Full year

  • 分子生物学実験

    2019.4 - 2019.9   First semester

▼display all

FD Participation

  • 2022.9   Role：Participation   Title：全学FD「M2Bシステムの使い方　～新機能を中心に紹介～」

    Organizer：University-wide

  • 2021.11   Role：Participation   Title：農学研究院FD「人を対象とする生命科学・医学系研究に関する倫理指針」について

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2021.7   Role：Participation   Title：農学研究院FD「科研費を獲りにいこう！　科研費獲得の技術と工夫」

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2020.12   Role：Participation   Title：大学の研究評価の現状と農学研究院の「部局独自の評価基準」案における業績分析

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2020.9   Role：Participation   Title：M2B学習支援システム講習会（オンライン開催）◇初級編・中級編◇10:00～12:00

    Organizer：University-wide

  • 2020.9   Role：Participation   Title：科研費を獲りにいこう！　勝ち抜く気合と技術

    Organizer：[Undergraduate school/graduate school/graduate faculty]

▼display all

Social Activities

  • 小中学生に向けた体験実験

    九州大学未来化学創造センター / 九州先端科学技術研究所(ISIT) 福岡市産学連携交流センター指定管理者[西鉄ビルマネージメント株式会社  福岡市産学連携交流センター   2023.7

    　More details

    Audience： General,　Scientific,　Company,　Civic organization,　Governmental agency

    Type：Lecture