Updated on 2024/10/31

Information

 

写真a

 
CHAKRABORTY TAPAS
 
Organization
Faculty of Agriculture Department of Bioresource Sciences Assistant Professor
School of Agriculture Department of Bioresource and Bioenvironment（Concurrent）
Graduate School of Bioresource and Bioenvironmental Sciences Department of Bioscience and Biotechnology（Concurrent）
Title
Assistant Professor
Tel
0955517470
Profile
Overall improvement of globally acceptable socio-economically sound aqua-sector
External link

Research Areas

  • Life Science / Cell biology

  • Life Science / Developmental biology

  • Environmental Science/Agriculture Science / Conservation of biological resources

  • Life Science / Aquatic life science

  • Life Science / Morphology and anatomical structure

  • Life Science / Laboratory animal science

  • Life Science / Laboratory animal science

  • Life Science / Animal production science

  • Life Science / Animal physiological chemistry, physiology and behavioral biology

  • Life Science / Animal physiological chemistry, physiology and behavioral biology

  • Life Science / Animal physiological chemistry, physiology and behavioral biology

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Research History

  • Kyushu University Assistant professor

    2020.4 - Present

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Research Interests・Research Keywords

  • Research theme：Biotechnology

    Keyword：Biotechnology

    Research period： 2024

  • Research theme：Fisheries and aquaculture

    Keyword：Fisheries and aquaculture

    Research period： 2024

  • Research theme：Stem cell biology

    Keyword：Stem cell biology

    Research period： 2024

  • Research theme：Sexual disorder

    Keyword：Sexual disorder

    Research period： 2024

  • Research theme：Sexual development

    Keyword：Sexual development

    Research period： 2024

  • Research theme：Reproduction

    Keyword：Reproduction

    Research period： 2024

  • Research theme：Omics

    Keyword：Omics

    Research period： 2024

  • Research theme：Molecular biology

    Keyword：Molecular biology

    Research period： 2024

  • Research theme：Medaka

    Keyword：Medaka

    Research period： 2024

  • Research theme：Controlled fish reproductive management

    Keyword：Stem cell, Next generation breeding, Aquaculture, ICT

    Research period： 2020.4 - 2025.3

Awards

  • Best Poster award

    2023.6   12th ISRPF, Greece  

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  • Best Oral presentation on Genome to Phenome in aquatics

    2022   Rashi-2022, India.  

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  • Best Publication of the Year (2014-2015)

    2015.6   Central Institute of Fisheries Education (CIFE), Mumbai, India  

  • Dr. Jalihal Endowment Award for the Best Dissertation (2006-2007)

    2007.8   Central Institute of Fisheries Education (CIFE), Mumbai, India  

Papers

  • Modeling the SDF-1/CXCR4 protein using advanced artificial intelligence and antagonist screening for Japanese anchovy Reviewed International journal

    Yahiro Issei# , Barnuevo Kyle Dominic Eguid# , Sato Oga# , Mohapatra Sipra , Toyoda Atsushi , Itoh Takehiko , Ohno Kaoru , Matsuyama Michiya , Chakraborty Tapas , Ohta Kohei

    Frontiers in Physiology   15   1349119   2024.1   （ ISSN:1664-042X eISSN:1664-042X ）

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

    SDF-1/CXCR4 chemokine signaling are indispensable for cell migration, especially the Primordial Germ Cell (PGC) migration towards the gonadal ridge during early development. We earlier found that this signaling is largely conserved in the Japanese anchovy (Engraulis japonicus, EJ), and a mere treatment of CXCR4 antagonist, AMD3100, leads to germ cell depletion and thereafter gonad sterilization. However, the effect of AMD3100 was limited. So, in this research, we scouted for CXCR4 antagonist with higher potency by employing advanced artificial intelligence deep learning-based computer simulations. Three potential candidates, AMD3465, WZ811, and LY2510924, were selected and in vivo validation was conducted using Japanese anchovy embryos. We found that seven transmembrane motif of EJ CXCR4a and EJ CXCR4b were extremely similar with human homolog while the CXCR4 chemokine receptor N terminal (PF12109, essential for SDF-1 binding) was missing in EJ CXCR4b. 3D protein analysis and cavity search predicted the cavity in EJ CXCR4a to be five times larger (6,307 Å³) than that in EJ CXCR4b (1,241 Å³). Docking analysis demonstrated lower binding energy of AMD3100 and AMD3465 to EJ CXCR4a (Vina score −9.6) and EJ CXCR4b (Vina score −8.8), respectively. Furthermore, we observed significant PGC mismigration in microinjected AMD3465 treated groups at 10, 100 and 1 × 10<sup>5</sup> nM concentration in 48 h post fertilized embryos. The other three antagonists showed various degrees of PGC dispersion, but no significant effect compared to their solvent control at tested concentrations was observed. Cumulatively, our results suggests that AMD3645 might be a better candidate for abnormal PGC migration in Japanese anchovy and warrants further investigation.

    DOI： 10.3389/fphys.2024.1349119

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  • Sex lethal gene manipulates gonadal development of medaka, Oryzias latipes through estrogenic interventions. International journal of molecular sciences. Invited Reviewed International journal

    Tapas Chakraborty Sipra Mohapatra, Michiya Matsuyama, Yoshitaka Nagahama, Kohei Ohta.

    International journal of molecular sciences.   2022.10

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  • Divulging the social sex change mechanism in a unique model system for studying the sexual plasticity of protogynous hermaphrodite fish, three bamboo leaf wrasse (Pseudolabrus sieboldi). Reviewed International journal

    Tapas Chakraborty, Yume Yamamoto#, Shoma Hanai#, Mayumi Hirano#, Sipra Mohapatra, Akihiko Yamaguchi, Tatsusuke Takeda, Michiya Matsuyama, Kohei Ohta.

    Frontiers in Marine Sciences   2022.10

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Sex determination, gonadal sex differentiation, and plasticity in vertebrate species Invited Reviewed International journal

    Yoshitaka Nagahama, Tapas Chakraborty, Bindhu Paul-Prasanth, Kohei Ohta, and Masaru Nakamura

    Physiological review   2021.6

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  • Estrogen and estrogen receptors chauffeur the sex-biased autophagic action in liver Reviewed International journal

    Sipra Mohapatra, Tapas Chakraborty, Sonoko Shimizu, Kayoko Ohta, Yoshitaka Nagahama, Kohei Ohta

    Cell death and Differentiation   2020.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41418-020-0567-3

  • Estrogen Receptor β2 Oversees Germ Cell Maintenance and Gonadal Sex Differentiation in Medaka, Oryzias latipes Reviewed

    13 ( 2 )   419 - 433   2019.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    In vertebrates, estrogen receptors are essential for estrogen-associated early gonadal sex development. Our previous studies revealed sexual dimorphic expression of estrogen receptor β2 (ERβ2) during embryogenesis of medaka, and here we investigated the functional importance of ERβ2 in female gonad development and maintenance using a transgenerational ERβ2-knockdown (ERβ2-KD) line and ERβ2-null mutants. We found that ERβ2 reduction favored male-biased gene transcription, suppressed female-responsive gene expression, and affected SDF1a and CXCR4b co-assisted chemotactic primordial germ cell (PGC) migration. Co-overexpression of SDF1a and CXXR4b restored the ERβ2-KD/KO associated PGC mismigration. Further analysis confirmed that curtailment of ERβ2 increased intracellular Ca2+ concentration, disrupted intra- and extracellular calcium homeostasis, and instigated autophagic germ cell degradation and germ cell loss, which in some cases ultimately affected the XX female sexual development. This study is expected improve our understanding of germ cell maintenance and sex spectrum, and hence open new avenues for reproductive disorder management. In this article, Chakraborty et al. find that estrogen receptor β2 (ERβ2) plays multifaceted crucial roles in medaka gonadal development and sexuality. ERβ2 predominantly expresses in germ cells, influences the SDF1/CXCR4 chemotaxis, PGC migration, and death, and regulates germ cell proliferation and various sex-biased gene transcriptions that are essential for gonadal sex assignment and maintenance.

    DOI： 10.1016/j.stemcr.2019.07.013

  • Hatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision Reviewed

    Tapas Chakraborty, Sipra Mohapatra, Megumi Tobayama, Kayoko Ohta, Yong Woon Ryu, Yukinori Kazeto, Kohei Ohta, Linyan Zhou, Yoshitaka Nagahama, Takahiro Matsubara

    Scientific reports   7 ( 1 )   2017.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41598-017-03314-7

  • Dmy initiates masculinity by altering Gsdf/Sox9a2/Rspo1 expression in medaka (Oryzias latipes) Reviewed

    Tapas Chakraborty, Lin Yan Zhou, Aparna Chaudhari, Taisen Iguchi, Y. Nagahama

    Scientific reports   6   2016.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/srep19480

  • Exposure effects of benzalkonium chloride (BAC) on gonadal physiology and fertility suppression in medaka (Oryzias latipes). Reviewed International journal

    Koki Mukai, Sipra Mohapatra, Michiya Matsuyama, Kohei Ohta, Tapas Chakraborty

    Environmental pollution (Barking, Essex : 1987)   361   124778 - 124778   2024.11   （ ISSN:0269-7491 eISSN:1873-6424 ）

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    Benzalkonium chloride (BAC), a commonly used quaternary ammonium compound in various products like antiseptics, cosmetics, and disinfectants, has raised concerns due to its potential to contaminate aquatic environments and subsequently affect the reproductive performance of the organisms within those ecosystems. The article underscores a critical concern regarding the impact of BAC on aquatic ecosystems, particularly its effect on fish reproductive quality, using medaka (Oryzias latipes) as a model organism. Firstly, while measuring lethal dose of BAC in adult medaka, we observed a dose dependent mortality in BAC treated fish (100 and 200 ppm: 100%; 60 ppm: 51.7%; 30 ppm or less: no mortality at 24 h post treatment (hpt)) and calculated the LD50 at 96 hpt as 39.291 ppm (95% confidence interval: 28.817-53.570 ppm). Further, we assessed the molecular, cellular and histological changes through long-term exposure. Enlarged sperm pockets and reduced spermatocyte were seen in BAC exposed testis while no significant structural changes were observed in the ovaries. Following BAC exposure, drastic alterations in the gene expression and cellular localization related to sex, estrogen signaling, and autophagy were also noted from gonads and liver. Subsequently, using a short-term exposure analysis, we confirmed the sex and time responsive transcriptional kinetics and found that BAC sequentially affected the gonadal somatic cells followed by germ cell differentiation. Finally, using reproductively competent male and female medaka, we conducted progeny production and performance analysis and depicted a drastic reduction in fecundity, and fertilization and hatching rate, indicating adverse effects of BAC on reproductive success. Cumulatively, these findings emphasize the consequences of widespread use of BAC on reproductive security of aquatic animals and highlights the need for further research to comprehend the potential harm posed by such compounds to aquatic animal health and ecosystem integrity.

    DOI： 10.1016/j.envpol.2024.124778

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  • <i>Star1</i> gene mutation reveals the essentiality of 11-ketotestosterone and glucocorticoids for male fertility in Nile Tilapia (<i>Oreochromis niloticus</i>)

    Su, Y; Wu, Y; Ye, ML; Zhao, CH; Li, L; Cai, J; Chakraborty, T; Yang, LY; Wang, DS; Zhou, LY

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY   273   110985   2024.8   （ ISSN:1096-4959 eISSN:1879-1107 ）

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    Language：English   Publisher：Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology  

    Steroidogenic acute regulatory protein (Star) plays an essential role in the biosynthesis of corticosteroids and sex steroids by mediating the transport of cholesterol from the outer to the inner membrane of mitochondria. Two duplicated Star genes, namely star1 and star2, have been identified in non-mammalian vertebrates. To investigate the roles of star genes in fish steriodogenesis, we generated two mutation lines of star1−/− and star1−/−/star2−/− in Nile tilapia (Oreochromis niloticus). Previous studies revealed that deficiency of star2 gene caused delayed spermatogenesis, sperm apoptosis and sterility in male tilapia. Our present data revealed that mutation of star genes impaired male fertility. Disordered seminiferous lobules and spermatic duct obstruction were found in the testis of both types of mutants. Moreover, significant decline in semen volume, sperm abnormality and impaired fertility were also detected in star1−/− and star1−/−/star2−/− males. In star1−/− male fish, lipid accumulation, up-regulation of steroidogenic enzymes, and significant decline of androgens were found. Additionally, hyperplasic interrenal cells, elevated steroidogenic gene expression level and decline of serum glucocorticoids were detected in star1 mutants. Intriguingly, either 11-KT or cortisol supplementation successfully rescued the impaired fertility of the star1−/− mutants. Taken together, these results further indicate that Star1 might play critical roles in the production of both 11-KT and glucocorticoids, which are indispensable for the maintenance of male fertility in fish.

    DOI： 10.1016/j.cbpb.2024.110985

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  • Insights into epigenetic regulation of cyp19a via comparative analysis using the scombrid chub mackerel as model Reviewed

    Mariel Galotta, Anh Tuan Dam, Yuhei Eto, Atsushi Toyoda, Takehiko Itoh, Sipra Mohapatra, Yukiko Ogino, Marie Saitou, Michiya Matsuyama, Tapas Chakraborty, Kohei Ohta

    Frontiers in Marine Science   11   2024.6   （ eISSN:2296-7745 ）

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    Authorship：Corresponding author   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

    Sexual development and reproduction are largely linked to epigenetic changes in many fish species. However, understanding of epigenetic regulation in scombrid species, such as tunas and mackerels, is limited. This study investigates DNA methylation’s impact on cyp19a expression, crucial for estrogen synthesis, focusing on chub mackerel. Given the commercial significance of scombrids and susceptibility of marine fish to environmental changes, elucidating epigenetic mechanisms, particularly in the context of global warming, becomes imperative. We aimed to generalize observations from chub mackerel to other scombrids. Additionally, we studied DNA methylation patterns across fish with different sexual systems to understand aromatase regulation’s phenotypic plasticity. Our in silico analysis revealed highly conserved promoter sequences within scombrids, sharing TFBS like Foxl2, FOS::JUN, ESRR, and Sox3, while CpG content varies. This indicates a conserved regulatory network controlling gene expression. We found sexual dimorphism in DNA methylation, with males hypermethylated and aromatase expression downregulated. Despite similar dnmt1 expression, tet1, tet2, and tet3 were higher expressed in females, suggesting that the observed DNA methylation patterns are maintained through active demethylation rather than differential methylation. Gonochoristic Japanese anchovy and protogynous bamboo leaf wrasse displayed similar trends, but species-specific methylation patterns highlight DNA methylation’s complex role in gonadal changes. In vitro assays confirmed methylation’s regulatory role and identified an SF-1 binding site relevant for promoter activation in chub mackerel. Another studied SF-1 site, present in both chub mackerel and bamboo leaf wrasse, showed regulatory effects, indicating potential similar regulatory mechanisms for cyp19a expression. Overall, our findings suggest that while global methylation affects cyp19a transcription, the variation in CpG density and location could be introducing nuances in its epigenetic regulation. This study contributes to our understanding of the cyp19a regulation in fish gonad maturation.

    DOI： 10.3389/fmars.2024.1408561

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  • SDF-1/CXCR4 signal is involved in the induction of Primordial Germ Cell migration in a model marine fish, Japanese anchovy (Engraulis japonicus) Reviewed International journal

    Issei Yahiro#, Oga Sato# , Sipra Mohapatra, Koki Mukai, Atsushi Toyoda , Takehiko Itoh, Michiya Matsuyama, Tapas Chakraborty, Kohei Ohta

    General and comparative endocrinology   351   114476 - 114476   2024.1   （ ISSN:0016-6480 eISSN:1095-6840 ）

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.ygcen.2024.114476

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  • Divulging the social sex change mechanism in a unique model system for studying the sexual plasticity of protogynous hermaphrodite fish, three bamboo leaf wrasse (Pseudolabrus sieboldi)

    Tapas Chakraborty, Yume Yamamoto, Shoma Hanai, Mayumi Hirano, Sipra Mohapatra, Akihiko Yamaguchi, Tatsusuke Takeda, Michiya Matsuyama, Kohei Ohta

    Frontiers in Marine Science   9   2022.12   （ eISSN:2296-7745 ）

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    Authorship：Lead author,　Corresponding author   Publishing type：Research paper (scientific journal)  

    The gonadal sexual fate of vertebrates is either defined by genetics or environment, or a combination of both factors. Interestingly, in sequential hermaphroditism, the animal can undergo natural sex changes from female-to-male, male-to-female, and bidirectional way throughout their lives. This change exhibits the process which shifts between oogenesis and spermatogenesis and is regarded as an ideal instance of sexual plasticity. To develop the experimental model for studying the sexual plasticity of protogynous fish, the social conditions that induce sex changes were defined in wrasse, Pseudolabrus sieboldi. When six females were kept together in a tank, the largest female became a male, whereas a similar conversion did not occur when only two females were present in a tank. A semi-gonadectomy analysis developed in the present study verified the direct relationship between gonadal sex and body coloration. In P. sieboldi, the sex change is controlled by the relative body size of an individual within a group, rather than by absolute body size. When six females were kept in smaller sized tanks, delayed sex change or unchanged individuals was observed. Overall, more than 90% of the largest females demonstrated sex change after being housed with five smaller females in different sizes of tanks ranging from 80 to 500 L. Furthermore, the experiment using a transparent barrier suggested that visual stimuli are one of the major cues to initiate sex change. Our findings on the laboratory conditions leading to the initiation of sex change in wrasse suggest the usefulness of this species as a model organism for comparative studies in molecular, cellular, and physiological mechanisms of sexual plasticity, as well as on social and reproductive behaviors.

    DOI： 10.3389/fmars.2022.1048506

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  • Sex Lethal Gene Manipulates Gonadal Development of Medaka, Oryzias latipes, through Estrogenic Interventions. Reviewed International journal

    Tapas Chakraborty, Sipra Mohapatra, Michiya Matsuyama, Yoshitaka Nagahama, Kohei Ohta

    International journal of molecular sciences   23 ( 24 )   2022.12   （ ISSN:16616596 eISSN:1422-0067 ）

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    Germ cells are pivotal for gonadal sexuality maintenance and reproduction. Sex lethal (sxl), the somatic sex determining gene of Drosophila, is the known regulator and initiator of germ cell femininity in invertebrates. However, the role of the Sxl homologue has rarely been investigated in vertebrates. So, we used medaka to clarify the role of sxl in vertebrate gonadogenesis and sexuality and identified two Sxl homologues, i.e., Sxl1a and Sxl1b. We found that sxl1a specifically expresses in the primordial germ cells (PGC), ovary, (early gonia and oocytes), while sxl1b distributions are ubiquitous. An mRNA overexpression of sxl1a accelerated germ cell numbers in 10 DAH XY fish, and sxl1a knockdown (KD), on the other hand, induced PGC mis-migration, aberrant PGC structuring and ultimately caused significant germ cell reduction in XX fish. Using an in vitro promoter analysis and in vivo steroid treatment, we found a strong link between sxl1a and estrogenic germ cell-population maintenance. Further, using sxl1a-KD and erβ2-knockout fish, we determined that sxl1 acts through erβ2 and controls PGC sexuality. Cumulatively, our study highlights the novel role of sxl1a in germ cell maintenance and sexual identity assignment and thus might become a steppingstone to understanding the commonalities of animal sexual development.

    DOI： 10.3390/ijms232415496

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  • Cyp17a2 is involved in testicular development and fertility in male Nile tilapia, <i>Oreochromis niloticus</i>

    Yang, LY; Wu, Y; Su, Y; Zhang, XF; Chakraborty, T; Wang, DS; Zhou, LY

    FRONTIERS IN ENDOCRINOLOGY   13   1074921   2022.11   （ ISSN:1664-2392 ）

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    Language：English   Publisher：Frontiers in Endocrinology  

    Background: Steroid hormones play an essential role in many reproductive processes of vertebrates. Previous studies revealed that teleost-specific Cyp17a2 (cytochrome P450 family 17 subfamily a 2) might be required for the production of cortisol in the head-kidney and 17α,20β-dihydroxy-4-pregnen-3-one (DHP) in ovary during oocyte maturation. However, the role of Cyp17a2 in male reproduction remains to be largely unknown. The aim of this study was to explore the essentiality of cyp17a2 gene in male steroidogenesis, spermatogenesis, and male fertility. Methods: A homozygous mutation line of cyp17a2 gene was constructed in tilapia by CRISPR/Cas9 gene editing technology. The expression level of germ cell and meiosis-related genes and steroidogenic enzymes were detected by qRT-PCR, IHC, and Western blotting. EIA and LC-MS/MS assays were used to measure the steroid production levels. And sperm quality was examined by Sperm Quality Analyzer software. Results: In this study, cyp17a2 gene mutation resulted in the significant decline of serum DHP and cortisol levels. On the contrary, significant increases in intermediate products of cortisol and DHP were found in cyp17a2-/- male fish. The deficiency of cyp17a2 led to the arrest of meiotic initiation in male fish revealing as the reduction of the expression of germ cell-related genes (vasa, piwil, oct4) and meiosis-related genes (spo11 and sycp3) by 90 dah. Afterwards, spermatogenesis was gradually recovered with the development of testis in cyp17a2-/- males, but it showed a lower sperm motility and reduced fertility compared to cyp17a2+/+ XY fish. Deletion of cyp17a2 led to the abnormal upregulation of steroidogenic enzymes for cortisol production in the head-kidney. Moreover, unaltered serum androgens and estrogens, as well as unchanged related steroidogenic enzymes were found in the testis of cyp17a2-/- male fish. Conclusion: This study proved that, for the fist time, Cyp17a2 is indispensable for cortisol and DHP production, and cyp17a2 deficiency associated curtailed meiotic initiation and subfertility suggesting the essentiality of DHP and cortisol in the male fertility of fish.

    DOI： 10.3389/fendo.2022.1074921

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  • CRISPR/Cas Genome Editing—Can It Become a Game Changer in Future Fisheries Sector? Reviewed

    Suvra Roy, Vikash Kumar, Bijay Kumar Behera, Janmejay Parhi, Sipra Mohapatra, Tapas Chakraborty, Basanta Kumar Das

    Frontiers in Marine Science   9   2022.7   （ eISSN:2296-7745 ）

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    Authorship：Corresponding author   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

    Fisheries and aquaculture are the fastest-growing food-producing sector and rapidly becoming an important element for the global food security since they are the primary source of seafood and high animal protein in the human diet. Genome editing offers new possibilities such as the clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein (Cas9) technology, which has the potential to accelerate the sustainable genetic improvement in fisheries and aquaculture. The CRISPR/Cas9 system has four key components, namely, target DNA, Cas9, the protospacer adjacent motif sequence, and the guide RNA or single-guide RNA. CRISPR/Cas is cheaper, easier, and more precise than the other genome editing technologies and can be used as a new breeding technology in fisheries and aquaculture to solve the far-reaching challenges. The attributes like high fecundity, external fertilization, short generation interval, the established method of breeding, and the larval rearing of most aquaculture species have advantages for CRISPR/Cas9 genome editing applications. CRISPR/Cas9 has recently been applied to the traits valued in some aquaculture species (almost &amp;gt;20 species), targeting the main traits of traditional genetic improvement initiatives like growth, disease resistance, reproduction, sterility, and pigmentation. Genome editing can fast forward the breeding process with precision where changes occur in the targeted genes. The probability of desired changes occurring and passing the trait in the next generation is high, so it takes 1-3 generations to establish a breed. Moreover, CRISPR/Cas genome editing rapidly introduces favorable changes by disrupting genes with targeted minor changes, in contrast to transgenesis, which introduces foreign genes into the host genome and thereby alleviates major public concerns on safety. Although the CRISPR/Cas technology has a tremendous potential, there are several technical challenges and regulatory and public issues concerning the applications in fisheries and the aquaculture breeding sector. Nonetheless, the exciting point in the CRISPR/Cas9 genome editing is that two CRISPR-edited fish, namely, red sea bream and tiger puffer developed by the Kyoto-based startup got approval and are now on the market for sale, and another fish, FLT-01 Nile tilapia developed by the AquaBounty, is not classified under genetically modified organism regulatory. However, there is still a way to go before it revolutionizes and becomes viable in commercial aquaculture as the new breeding technology for aquaculture-important traits and species.

    DOI： 10.3389/fmars.2022.924475

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  • Genome Editing in Fish Reproduction Invited Reviewed International journal

    Sipra Mohapatra, Tapas Chakraborty

    2022.6

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  • The role of StAR2 gene in testicular differentiation and spermatogenesis in Nile tilapia (Oreochromis niloticus) Reviewed International journal

    u Li, You Wu, Chenhua Zhao, Yiyang Miao, Jing Cai, Lingyun Song, Jing Wei, Tapas Chakraborty, Limin Wu, Deshou Wang, Linyan Zhou,

    The Journal of Steroid Biochemistry and Molecular Biology   2021.8

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    DOI： https://doi.org/10.1016/j.jsbmb.2021.105974

  • Effects of long term antiprogestine mifepristone (RU486) exposure on sexually dimorphic lncRNA expression and gonadal masculinization in Nile tilapia (Oreochromis niloticus) Reviewed

    Jing Cai, Lu Li, Lingyun Song, Lang Xie, Feng Luo, Shaohua Sun, Tapas Chakraborty, Linyan Zhou, Deshou Wang

    Aquatic Toxicology   215   2019.10

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    DOI： 10.1016/j.aquatox.2019.105289

  • Molecular cloning, characterization and expression analysis of complement components in red sea bream (Pagrus major) after Edwardsiella tarda and red sea bream Iridovirus (RSIV) challenge Reviewed

    Mohammad Ali Noman Reza, Sipra Mohapatra, Sonoko Shimizu, Shin Ichi Kitamura, Shogo Harakawa, Hidemasa Kawakami, Kei Nakayama, Eitaro Sawayama, Takahiro Matsubara, Kohei Ohta, Tapas Chakraborty

    Fish and Shellfish Immunology   82   286 - 295   2018.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.fsi.2018.08.027

  • Comparative transcriptome profiling and characterization of gene expression for ovarian differentiation under RU486 treatment Reviewed

    Shaohua Sun, Jing Cai, Wenjing Tao, Limin Wu, Chakraborty Tapas, Linyan Zhou, Deshou Wang

    General and Comparative Endocrinology   261   166 - 173   2018.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ygcen.2018.03.005

  • Short-term starvation and realimentation helps stave off Edwardsiella tarda infection in red sea bream (Pagrus major) Reviewed

    Sipra Mohapatra, Tapas Chakraborty, Mohammad Ali Noman Reza, Sonoko Shimizu, Takahiro Matsubara, Kohei Ohta

    Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology   206   42 - 53   2017.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.cbpb.2017.01.009

  • Blockage of progestin physiology disrupts ovarian differentiation in XX Nile tilapia (Oreochromis niloticus) Reviewed

    Linyan Zhou, Feng Luo, Xuelian Fang, Tapas Charkraborty, Limin Wu, Jing Wei, Deshou Wang

    Biochemical and Biophysical Research Communications   473 ( 1 )   29 - 34   2016.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrc.2016.03.045

  • Rspo1-activated signalling molecules are sufficient to induce ovarian differentiation in XY medaka (Oryzias latipes) Reviewed

    Linyan Zhou, Tapas Charkraborty, Qian Zhou, Sipra Mohapatra, Yoshitaka Nagahama, Yueguang Zhang

    Scientific reports   6   2016.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/srep19543

  • Starvation beneficially influences the liver physiology and nutrient metabolism in Edwardsiella tarda infected red sea bream (Pagrus major) Reviewed

    Sipra Mohapatra, Tapas Chakraborty, Sonoko Shimizu, Shintaro Urasaki, Takahiro Matsubara, Yoshitaka Nagahama, Kohei Ohta

    Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology   189   1 - 10   2015.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.cbpa.2015.07.003

  • Figla favors ovarian differentiation by antagonizing spermatogenesis in a teleosts, Nile tilapia (Oreochromis niloticus) Reviewed

    Yongxiu Qiu, Shaohua Sun, Tapas Charkraborty, Limin Wu, Lina Sun, Jing Wei, Yoshitaka Nagahama, Deshou Wang, Linyan Zhou

    PloS one   10 ( 4 )   2015.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1371/journal.pone.0123900

  • Steroid responsive regulation of IFNγ2 alternative splicing and its possible role in germ cell proliferation in medaka Reviewed

    400   61 - 70   2015.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    Interferon gamma (. IFNγ) is an active player in estrogen dependent immuno-regulation of fish. The present work was aimed to characterize the alternatively spliced isoforms of IFNγ2 in the gonadal sex development in medaka. Phylogenetic analysis demonstrated that IFNγ2a and 2b were clustered with fish specific interferon gamma. Our in vitro promoter and mini-genome analysis data confirmed that alternative splicing of IFNγ2 is regulated by estrogens and androgens. Tissue distribution, quantitative PCR and ISH data demonstrated ubiquitous expression of IFNγ2a, while IFNγ2b was only expressed predominantly in female germ cells than males. This was further confirmed by germ cell specific GFP signals in the IFNγ2b-GFP over-expressed embryos and specific induction of IFNγ2b expression in the BrdU positive cells. All together our data suggest that steroid responsive alternatively spliced IFNγ2b isoforms might have some indirect roles in germ cell proliferation and thus can be an important candidate for immuno-reproductive interaction studies.

    DOI： 10.1016/j.mce.2014.10.012

  • Dietary Multispecies Probiotic Supplementation Enhances the Immunohematological Responses and Reduces Mortality by Aeromonas hydrophila in Labeo rohita Fingerlings Reviewed

    Sipra Mohapatra, Tapas Chakraborty, Ashisa Kumar Prusty, Kurucheti Pani Prasad, Kedar Nath Mohanta

    Journal of the World Aquaculture Society   45 ( 5 )   532 - 544   2014.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/jwas.12144

  • Beneficial effects of dietary probiotics mixture on hemato-immunology and cell apoptosis of Labeo rohita fingerlings reared at higher water temperatures Reviewed

    Sipra Mohapatra, Tapas Chakraborty, Ashisa K. Prusty, Kurchetti PaniPrasad, Kedar N. Mohanta

    PloS one   9 ( 6 )   2014.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1371/journal.pone.0100929

  • Characterization of two paralogous StAR genes in a teleost, Nile tilapia (Oreochromis niloticus) Reviewed

    Xiangguo Yu, Limin Wu, Lang Xie, Shijie Yang, Tapas Charkraborty, Hongjuan Shi, Deshou Wang, Linyan Zhou

    Molecular and Cellular Endocrinology   392 ( 1-2 )   152 - 162   2014.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.mce.2014.05.013

  • Aquaculture and stress management A review of probiotic intervention Reviewed

    S. Mohapatra, T. Chakraborty, V. Kumar, G. Deboeck, K. N. Mohanta

    Journal of Animal Physiology and Animal Nutrition   97 ( 3 )   405 - 430   2013.6

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/j.1439-0396.2012.01301.x

  • R-spondins are involved in the ovarian differentiation in a teleost, medaka (Oryzias latipes) Reviewed

    Linyan Zhou, Tapas Charkraborty, Xiangguo Yu, Limin Wu, Gang Liu, Sipra Mohapatra, Deshou Wang, Yoshitaka Nagahama

    BMC Developmental Biology   12   2012.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1186/1471-213X-12-36

  • Fenvalerate induced stress mitigation by dietary supplementation of multispecies probiotic mixture in a tropical freshwater fish, Labeo rohita (Hamilton) Reviewed

    Sipra Mohapatra, Tapas Chakraborty, Ashisa Kumar Prusty, Kundan Kumar, K. Pani Prasad, Kedar Nath Mohanta

    Pesticide Biochemistry and Physiology   104 ( 1 )   28 - 37   2012.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.pestbp.2012.06.006

  • Use of different microbial probiotics in the diet of rohu, Labeo rohita fingerlings Effects on growth, nutrient digestibility and retention, digestive enzyme activities and intestinal microflora Reviewed

    S. Mohapatra, T. Chakraborty, A. K. Prusty, P. Das, K. Paniprasad, K. N. Mohanta

    Aquaculture Nutrition   18 ( 1 )   1 - 11   2012.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/j.1365-2095.2011.00866.x

  • Estrogen receptors in medaka (Oryzias latipes) and estrogenic environmental contaminants An in vitro-in vivo correlation Reviewed

    Tapas Chakraborty, Yoshinao Katsu, Lin Yan Zhou, Shinichi Miyagawa, Yoshitaka Nagahama, Taisen Iguchi

    Journal of Steroid Biochemistry and Molecular Biology   123 ( 3-5 )   115 - 121   2011.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.jsbmb.2010.11.015

  • Differential expression of three estrogen receptor subtype mRNAs in gonads and liver from embryos to adults of the medaka, Oryzias latipes Reviewed

    Tapas Chakraborty, Yasushi Shibata, Lin Yan Zhou, Yoshinao Katsu, Taisen Iguchi, Yoshitaka Nagahama

    Molecular and Cellular Endocrinology   333 ( 1 )   47 - 54   2011.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.mce.2010.12.002

  • GFP expressing bacterial biosensor to measure lead contamination in aquatic environment Reviewed

    Tapas Chakraborty, P. Gireesh Babu, Absar Alam, Aparna Chaudhari

    Current Science   94 ( 6 )   800 - 805   2008.3

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    Language：English   Publishing type：Research paper (scientific journal)  

▼display all

Books

  • Unveiling sex spectrum of sex changing fish gonad using single cell analysis

    Kohei Ohta, Tapas Chakraborty（ Role： Joint authorAgriculture biotechnology）

    2024.1 

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  • Single cell RNA-seq analysis to understand the sexual plasticity in the gonad of sex changing fishSingle cell RNA-seq analysis to understand the sexual plasticity in the gonad of sex changing fish

    Kohei Ohta, Tapas Chakraborty（ Role： Joint authorsingle cell omics）

    Fujisan.co.jp  2023.6 

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  • Genome Editing in Fish Reproduction

    Mohapatra S., Chakraborty T.

    Advances in Fisheries Biotechnology  2022.1    （ ISBN:9789811632150, 9789811632143 ）

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    Fish eggs are an excellent system for studying how cells organize dynamically into exceedingly complex structures. It is accessible to experimental and genetic manipulations and is easier to scrutinize the development from fertilization to hatching, ex vivo. Genome editing in fish eggs, though challenging, is a powerful technique to develop genetically edited animals with minimal changes to the whole genome and is a huge leap in achieving reproductive stability and fertility enhancement. Usually by applying different techniques like CRISPR/Cas9, which is a faster and cost-effective gene targeting technique, it is convenient to conduct reproductive manipulation study and also produce knockout (KO) and knockin models, which would give us an opportunity to obtain better understanding about fish reproduction or the reproductive behavior of several fish.

    DOI： 10.1007/978-981-16-3215-0_7

    Scopus

  • Bauhaus effects in art, architecture and design

    James-Chakraborty Kathleen, T. Kriebel Sabine

    Routledge  2022    （ ISBN:9781032205397 ）

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    Language：English  

    CiNii Books

  • Genome Editing in Fish Reproduction. In: Pandey, P.K., Parhi, J. (eds) Advances in Fisheries Biotechnology.

    Mohapatra Sipra, Chakraborty Tapas

    Springer Nature Singapore Pte Ltd.  2022    （ ISBN:9789811632150 ）

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    Total pages：20  

    CiNii Research

Presentations

  • Can fish be artificially equipped　with a secondary functional gonad? International conference

    2022.7 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • ゲノム編集により作出された“おとなしいマサバ”の形質評価.

    小川拓摩#・大賀浩史・Mohapatra Sipra・Chakraborty Tapas・太田耕平・長野直樹・長迫智也・内田誠一・藤原篤志・佐久間哲史・山本卓・松山倫也.

    日本水産増殖学会(JSAS),  2022.11 

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    Event date： 2023.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  • Genome-edited "modest mackerel" for aquaculture. International conference

    Takuma Ogawa#, Hirofumi Ohga, Tapas Chakraborty, Kohei Ohta, Naoki Nagano, Atsushi Fujiwara, Tomoya Nagasako, Seiichi Uchida, Tetsushi Sakuma, Takashi Yamamoto, Michiya Matsuyama.

    Responsible Aquaculture and Sustainable Fisheries Interact (RASHI).Tripura, India (2022)  2022.12 

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    Event date： 2023.6

    Language：Japanese  

    Country：India  

  • Genome-edited "modest mackerel" for aquaculture. International conference

    Takuma Ogawa, Hirofumi Ohga, Tapas Chakraborty, Kohei Ohta, Naoki Nagano, Atsushi Fujiwara, Tomoya Nagasako, Seiichi Uchida, Tetsushi Sakuma, Takashi Yamamoto, Michiya Matsuyama.

    Responsible Aquaculture and Sustainable Fisheries Interact (RASHI).Tripura, India (2022)  2022.12 

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    Event date： 2023.6

    Language：Japanese  

    Country：India  

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  • ゲノム編集により作出された“おとなしいマサバ”の形質評価.

    小川拓摩, 大賀浩史, Mohapatra Sipra, Chakraborty Tapas, 太田耕平, 長野直樹, 長迫智也, 内田誠一, 藤原篤志, 佐久間哲史, 山本卓, 松山倫也.

    日本水産増殖学会(JSAS),  2022.11 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

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  • SDF-1/CXCR4 signal is involved in the induction of PGC migration in a model marine fish, Japanese anchovy (Engraulis japonicus) International conference

    Yahiro Issei#, Chakraborty Tapas, Ohta Kohei, Matsuyama Michiya

    12th ISRPF  2023.5 

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    Event date： 2023.5

    Language：English  

    Country：Greece  

  • SDF-1/CXCR4 signal is involved in the induction of PGC migration in a model marine fish, Japanese anchovy (Engraulis japonicus) International conference

    Yahiro Issei, Chakraborty Tapas, Ohta Kohei, Matsuyama Michiya

    12th ISRPF  2023.5 

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    Event date： 2023.5

    Language：English  

    Venue：Greece   Country：Greece  

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  • マサバ黄体形成ホルモン（Lh）のノックアウトによる不妊化−妊性回復系統の作出の試み International conference

    江口瑛#・八尋逸清#・佐藤桜雅#・Mohapatra Sipra ・ 向井幸樹・伊藤武彦・長野直樹・松山倫也・Chakraborty Tapas ・太田耕平

    2024.3 

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    Event date： 2024.3

    Language：Japanese  

    Venue：Tokyo Univiersity of marine sciences   Country：Japan  

  • Gonadal stem cells in a sex changing fish, Pseudolabrus sieboldi.

    Kodai Mizumura, Tapas Chakraborty, Kazuki Tateyama, Shoma Hanai, Yoshihara Yuya, Yume Yamamoto, Sipra Mohapatra, Yumiko Yamasaki, Atsushi Toyota, Ito Takehiko, Yoshitaka Nagahama, Michiya Matsuyama, Kohei Ohta.

    2022.12 

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    Event date： 2023.6

    Language：English  

    Country：Japan  

  • Aquaculture food security through local innovation -a model case of mackerel aquaculture. Invited International conference

    Tapas Chakraborty.

    RASHI  2022.12 

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    Event date： 2023.6

    Language：English  

    Country：India  

  • DNA methylation patterning at regulatory regions of two gonadotropin genes in the marine cultured fish, chub mackerel (Scomber japonicus). International conference

    Mariel Galotta#, Tapas Chakraborty, Naoki Nagano, Takehiko Itoh , Michiya Matsuyama, Kohei Ohta.

    JSFS  2023.3 

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    Event date： 2023.6

    Language：English  

    Country：Japan  

  • ホシササノハベラ生殖腺の性転換に伴う細胞増殖動態と血中ステロイドの分泌パターン. International conference

    花井匠磨#・Tapas Chakraborty・松山倫也・長濱嘉孝. 太田耕平.

    2022.3 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • ホシササノハベラ生殖腺の性転換に伴う細胞増殖動態と血中ステロイドの分泌パターン. International conference

    花井匠磨#・Tapas Chakraborty・松山倫也・長濱嘉孝. 太田耕平.

    2023.3 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • マサバ生殖腺組織の筋内移植による配偶子形成.

    八尋逸清・Chakraborty Tapas・河崎敏広・酒井則良・太田耕平・松山倫也.

    2023.3 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • カタクチイワシをモデルとした性成熟・成長に関わるエピジェネティック制御機構の解析

    江藤 悠平・Tapas Chakraborty・北野 載 ・伊藤武彦・松山倫也・太田耕平.

    日本水産学会九州支部大会(2022)  2022.10 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • DNA methylation patterns at regulatory regions of reproduction related genes in chub mackerel, Scomber japonicus.

    2022.3 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • カイコ蛹を魚粉代替原料とした完全養殖マサバの成長および体成分. International conference

    山口怜良#・長野直樹#・Sipra Mohapatra・Tapas Chakraborty・ 向井幸樹・太田耕平・日下部宜宏・松山倫也.

    2022.3 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • ホシササノハベラの社会性による性転換に伴う脳下垂体の遺伝子発現動態.

    山本優芽・花井匠磨・Chakraborty Tapas・豊田敦・伊藤武彦・松山倫也・長濱嘉孝・太田耕平.

    2022.10 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

  • Involvement of the pituitary gland in socially controlled sex change. International conference

    Yume Yamamoto#, Shoma Hanai#, Tapas Chakraborty, Astushi Toyoda, Takehiko Itoh, Michiya Matsuyama, Yoshitaka Nagahama, Kohei Ohta.

    2022.12 

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    Event date： 2023.6

    Language：English  

    Country：India  

  • DNA methylation patterning at regulatory regions of two gonadotropin genes in the marine cultured fish, chub mackerel (Scomber japonicus). International conference

    Mariel Galotta, Tapas Chakraborty, Naoki Nagano, Takehiko Itoh, Michiya Matsuyama, Kohei Ohta.

    JSFS  2023.3 

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    Event date： 2023.6

    Language：English  

    Country：Japan  

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  • マサバ生殖腺組織の筋内移植による配偶子形成.

    八尋逸清, Chakraborty Tapas, 河崎敏広, 酒井則良, 太田耕平, 松山倫也.

    2023.3 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

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  • ホシササノハベラ生殖腺の性転換に伴う細胞増殖動態と血中ステロイドの分泌パターン. International conference

    花井匠磨, Tapas Chakraborty, 松山倫也, 長濱嘉孝. 太田耕平.

    JSFS  2023.3 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

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  • ホシササノハベラ生殖腺の性転換に伴う細胞増殖動態と血中ステロイドの分泌パターン. International conference

    花井匠磨, Tapas Chakraborty, 松山倫也, 長濱嘉孝. 太田耕平.

    JSFS  2022.3 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

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  • ホシササノハベラの社会性による性転換に伴う脳下垂体の遺伝子発現動態.

    山本優芽, 花井匠磨, Chakraborty Tapas, 豊田敦, 伊藤武彦, 松山倫也, 長濱嘉孝, 太田耕平.

    2022.10 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

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  • カタクチイワシをモデルとした性成熟・成長に関わるエピジェネティック制御機構の解析

    江藤 悠平, Tapas Chakraborty, 北野 載, 伊藤武彦, 松山倫也, 太田耕平.

    日本水産学会九州支部大会(2022)  2022.10 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

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  • カイコ蛹を魚粉代替原料とした完全養殖マサバの成長および体成分. International conference

    山口怜良, 長野直樹, Sipra Mohapatra, Tapas Chakraborty, 向井幸樹, 太田耕平, 日下部宜宏, 松山倫也.

    2022.3 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

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  • Involvement of the pituitary gland in socially controlled sex change. International conference

    Yume Yamamoto, Shoma Hanai, Tapas Chakraborty, Astushi Toyoda, Takehiko Itoh, Michiya Matsuyama, Yoshitaka Nagahama, Kohei Ohta.

    2022.12 

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    Event date： 2023.6

    Language：English  

    Country：India  

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  • Gonadal stem cells in a sex changing fish, Pseudolabrus sieboldi.

    Kodai Mizumura, Tapas Chakraborty, Kazuki Tateyama, Shoma Hanai, Yoshihara Yuya, Yume Yamamoto, Sipra Mohapatra, Yumiko Yamasaki, Atsushi Toyota, Ito Takehiko, Yoshitaka Nagahama, Michiya Matsuyama, Kohei Ohta.

    2022.12 

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    Event date： 2023.6

    Language：English  

    Country：Japan  

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  • DNA methylation patterns at regulatory regions of reproduction related genes in chub mackerel, Scomber japonicus.

    Mariel Galotta, Tapas Chakraborty, Naoki Nagano, Takehiko Itoh, Michiya Matsuyama, Kohei Ohta.

    2022.3 

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    Event date： 2023.6

    Language：Japanese  

    Country：Japan  

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  • Molecular mechanism of sexual plasticity in a natural sex changing fish. International conference

    Ohta Kohei, Chakraborty Tapas, Tateyama Kazuki#, Hanai Shoma#, Mizumura Kodai#, Yamamoto Yume#, Mohapatra Sipra, Yamasaki Yumiko, Toyoda Atsushi, Itoh Takehiko, Matsuyama Michiya, Nagahama Yoshitaka

    12th ISRPF  2023.5 

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    Event date： 2023.5

    Language：English  

    Country：Greece  

  • Oct4 and sexuality in fish gonad; an understanding using Medaka, Oryzias latipes International conference

    Chakraborty Tapas, Mohapatra Sipra, Ohta Kohei, Matsuyama, Michiya, Nagahama Yoshitaka

    12th ISRPF  2023.5 

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    Event date： 2023.5

    Language：English  

    Country：Greece  

  • Molecular mechanism of sexual plasticity in a natural sex changing fish. International conference

    Ohta Kohei, Chakraborty Tapas, Tateyama Kazuki, Hanai Shoma, Mizumura Kodai, Yamamoto Yume, Mohapatra Sipra, Yamasaki Yumiko, Toyoda Atsushi, Itoh Takehiko, Matsuyama Michiya, Nagahama Yoshitaka

    12th ISRPF  2023.5 

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    Event date： 2023.5

    Language：English  

    Venue：Greece   Country：Greece  

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  • Oct4 and sexuality in fish gonad; an understanding using Medaka, Oryzias latipes International conference

    Chakraborty Tapas, Mohapatra Sipra, Ohta Kohei, Matsuyama, Michiya, Nagahama Yoshitaka

    12th ISRPF  2023.5 

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    Event date： 2023.5

    Language：English  

    Venue：Greece   Country：Greece  

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  • Physiological implication of fish meal substitution in aquaculturable fish; a study using chub mackerel, Scomber japonicus International conference

    Sipra Mohapatra, Tapas Chakraborty, Issei Yahiro#, Koki Mukai, Takehiko Itoh, Kohei Ohta, Michiya Matsuyama, Naoki Nagano

    JSFS  2022.3 

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    Event date： 2022.3

    Language：English   Presentation type：Oral presentation (general)  

    Country：Japan  

  • SDF- 1 /CXCR 4 シグナル阻害によるカタクチイワシの不妊化⼿法の開発 International conference

    ⼋尋逸清#・Tapas Chakraborty・太⽥耕平・松⼭倫也（九⼤院農）

    2022.3 

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    Event date： 2022.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  • DNA methylation patterning at regulatory regions of two gonadotropin genes in the marine cultured fish, chub mackerel (Scomber japonicus) International conference

    Mariel Galotta#, Tapas Chakraborty, Naoki Nagano, Takehiko Itoh , Michiya Matsuyama, Kohei Ohta

    JSFS  2022.3 

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    Event date： 2022.3

    Language：English   Presentation type：Oral presentation (general)  

    Country：Japan  

  • DNA methylation patterns at regulatory regions of reproduction related genes in chub mackerel, Scomber japonicus.

    JSFS  2022.1 

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    Event date： 2022.1

    Language：English   Presentation type：Oral presentation (general)  

    Country：Japan  

  • DNA methylation patterns at regulatory regions of reproduction related genes in chub mackerel, Scomber japonicus.

    Mariel Galotta, Tapas Chakraborty (Kyushu Univ.), Naoki Nagano (Miyazaki Univ.), Takehiko Itoh (Tokyo Institute of Technology), Michiya Matsuyama, Kohei Ohta (Kyushu Univ.)

    JSFS  2022.1 

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    Event date： 2022.1

    Language：English  

    Country：Japan  

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  • カイコ蛹を魚粉代替原料とした完全養殖マサバの成長および体成分

    山口怜良・長野直樹・Sipra Mohapatra・Tapas Chakraborty・ 向井幸樹・太田耕平・日下部宜宏・松山倫也

    2022.1 

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    Event date： 2022.1

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  • マサバ Scomber japonicus の自家生殖腺移植による次世代生産法の開発

    八尋逸清#・Tapas Chakraborty・太田耕平・松山倫也（九大院農）

    2022.1 

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    Event date： 2022.1

    Language：English  

    Country：Japan  

  • カタクチイワシをモデルとした性成熟・成長に関わるエピジェネティック制御機構の解析

    江藤 悠平・Tapas Chakraborty（九大院農）・北野 載（水産機構開発セ） 伊藤武彦（東工大生命理工）・松山倫也・太田耕平（九大院農）

    2022.1 

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    Event date： 2022.1

    Language：Japanese  

    Country：Japan  

  • ホシササノハベラ生殖腺の性転換に伴う細胞増殖動態と血中ステロイドの分泌パターン

    花井匠磨・Tapas Chakraborty・松山倫也（九大院農）・長濱嘉孝（基生研）太田耕平（九大院農）

    2022.1 

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    Event date： 2022.1

    Language：English  

    Country：Japan  

  • カタクチイワシをモデルとした性成熟・成長に関わるエピジェネティック制御機構の解析

    江藤 悠平, Tapas Chakraborty(九大院農), 北野 載(水産機構開発セ) 伊藤武彦(東工大生命理工), 松山倫也, 太田耕平(九大院農)

    JSFS  2022.1 

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    Event date： 2022.1

    Language：Japanese  

    Country：Japan  

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  • ホシササノハベラ生殖腺の性転換に伴う細胞増殖動態と血中ステロイドの分泌パターン

    花井匠磨, Tapas Chakraborty, 松山倫也(九大院農), 長濱嘉孝(基生研)太田耕平(九大院農)

    JSFS  2022.1 

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    Event date： 2022.1

    Language：English  

    Country：Japan  

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  • DNA methylation analysis ofregulatory regions for gonadotropins genes in marine cultured chub mackerel Scomber japonicus International conference

    Mariel Galotta#, Tapas Chakraborty, Takehiko Itoh, Naoki Nagano, Michiya Matsuyama, Kohei Ohta.

    Japan-norway bilateral symposium  2021.6 

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    Event date： 2021.6

    Language：English   Presentation type：Symposium, workshop panel (public)  

    Country：Japan  

  • Germline stem cells in the sex changing fish, Pseudolabrus sieboldi. International conference

    Ohta K, Mitome K#, Fujita J#, Chakraborty T, Mohapatra S, Matsuyama M,

    Inaugural Yanagimachi Symposium  2018.8 

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    Event date： 2020.8

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  • Estrogen Receptor β2 is required for germ cell migration and ovarian differentiation in genetically female medaka International conference

    2014.6 

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    Event date： 2020.8

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Portugal  

  • Sex determination/differentiation and sexual plasticity in medaka Invited International conference

    Tapas Chakraborty

    1st Ehime Medaka Forum  2015.10 

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    Event date： 2020.8

    Language：English   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Country：Japan  

  • Germ line stem cells and sexual plasticity in medaka, Oryzias latipes International conference

    Tapas Chakraborty, S. Mohapatra, K. Ohta, Y. Nagahama

    8th International Symposium on Fish Endocrinology (ISFE)  2016.6 

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    Event date： 2020.8

    Language：English   Presentation type：Oral presentation (general)  

    Country：Canada  

  • Major players in sex-biased regulation of autophagy in fish

    S. Mohapatra, Tapas Chakraborty, S. Shimizu, K. Ohta

    18th International Congress of Comparative Endocrinology (ICCE),  2016.6 

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    Event date： 2020.8

    Language：English   Presentation type：Oral presentation (general)  

    Country：Japan  

  • Sexual identity in fish: an understanding through estrogen-estrogen receptor regulation in medaka Invited International conference

    Tapas Chakraborty, S. Mohapatra, Y. Nagahama

    International Conference on Frontiers of Endocrinology, Chongqing, China  2016.6 

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    Event date： 2020.8

    Language：English   Presentation type：Oral presentation (general)  

    Country：China  

  • Sex specific expressions of oct4 (pou5f1) gene in gonadal germ cells of the hermaphrodite wrasse Pseudolabrus sieboldi and the gonochoristic anchovy Engraulis japonicas, International conference

    Fujita J#, Chakraborty T, Mohapatra S, Kurita K, Ryu YW, Matsubara T, Matsuyama M, Nagahama Y, Ohta K,

    JSFS,  2017.9 

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    Event date： 2020.8

    Language：English  

    Country：Japan  

  • Gender biasedness in autophagy: From a fish perspective Invited International conference

    S. Mohapatra, Tapas Chakraborty, S. Shimizu, T. Matsubara, K. Ohta

    International Symposium on Recent Advances in Comparative Endocrinology  2017.12 

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    Event date： 2020.8

    Language：English   Presentation type：Oral presentation (general)  

    Country：India  

  • Hatching enzymes and aberrant gonadal degeneration in fish International conference

    Tapas Chakraborty, S. Mohapatra, S. Shimizu, K. Ohta, Y. Nagahama

    International Symposium on Recent Advances in Comparative Endocrinology  2017.12 

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    Event date： 2020.8

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  • Exploring the potentiality of autophagy in fish fertility

    S. Mohapatra, Tapas Chakraborty, D. Pandey@, T. Matsubara, K. Ohta

    International Symposium on Reproductive Physiology of Fish (ISRPF), Manaus, Brazil  2018.6 

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    Event date： 2020.8

    Language：English  

    Country：Brazil  

  • マサバ Scomber japonicus の自家生殖腺移植による次世代生産法の開発

    I. Yahiro, Tapas Chakraborty, K. Ohta, M. Matsuyama

    JSFS spring Meeting, Tokyo, Japan, 2022  2022 

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  • SDF- 1 /CXCR 4 シグナル阻害によるカタクチイワシの不妊化⼿法の開発

    I. Yahiro, Tapas Chakraborty, K. Ohta, M. Matsuyama

    JSFS spring Meeting, Tokyo, Japan, 2022,  2022 

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    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Role of Oct4 in gonadal sex maintenance of fish

    Tapas Chakraborty, Yoshitaka Nagahama

    JSFS spring Meeting, Tokyo, Japan, 2024  2024 

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    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Physiological implication of fish meal substitution in aquaculturable fish; a study using chub mackerel, Scomber japonicus

    S. Mohapatra, Tapas Chakraborty, I. Yahiro, K. Mukai, T. Itoh, K. Ohta, M. Matsuyama, N. Nagano

    JSFS spring Meeting, Tokyo, Japan, 2022,  2022 

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    Presentation type：Oral presentation (general)  

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  • Gonadal stem cell: a possible solution of futuristic aquaculture

    Tapas Chakraborty, Michiya Matsuyama

    SFS spring Meeting, Tokyo, Japan, 2023  2023 

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    Language：English   Presentation type：Oral presentation (general)  

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  • DNA methylation patterning at regulatory regions of two gonadotropin genes in the marine cultured fish, chub mackerel (Scomber japonicus)

    M. Galotta, Tapas Chakraborty, T. Itoh, N. Nagano, M. Matusyama, K. Ohta

    JSFS spring Meeting, Tokyo, Japan, 2022  2022 

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    Presentation type：Oral presentation (general)  

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  • Can fish be artificially equipped with a secondary functional gonad?

    Issei Yahiro, Tapas Chakraborty, Kohei Ohta, Michiya Matsuyama

    30th CECE & 9th ISFE, Faro, Portugal, 2022  2022 

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    Language：English   Presentation type：Oral presentation (general)  

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  • Aquaculture food security through local innovation -a model case of mackerel aquaculture. Invited

    Tapas Chakraborty

    Responsible Aquaculture and Sustainable Fisheries Interact (RASHI). Tripura, India, 2022  2022.12 

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    Language：English   Presentation type：Oral presentation (invited, special)  

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MISC

  • Sex determination, gonadal sex differentiation, and plasticity in vertebrate species Reviewed

    Yoshitaka Nagahama, Tapas Chakraborty, Bindhu Paul-Prasanth, Kohei Ohta, and Masaru Nakamura

    Physiological reviews   2021.6

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    Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  • 性転換魚の生殖腺における性的可塑性とシングルセルRNA-seq解析の試み

    太田 耕平, Chakraborty Tapas

    細胞   55 ( 7 )   443 - 447   2023.6   （ ISSN:1346-7557 ）

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    Language：Japanese   Publisher：(株)ニュー・サイエンス社  

    哺乳類では,遺伝的に性が決まり,その性に従って性分化をした後,同じ性のまま一生を過ごす。一方,魚類のなかには,遺伝的に性を決定するもののみならず,環境の影響を受けて性が決まるものや,一生の間に性を転換させるものなど,様々な性決定/性分化/性転換の様式がみられる。なかでも生息環境における社会性によって性をかえる性転換魚は,社会環境の変化を起点として脳や生殖腺を含む個体全体の性を転換させることから,脊椎動物における個体,器官(脳や生殖腺),および細胞の性的可塑性を理解する上での格好の研究材料となる。これまでに筆者らは性転換魚の実験系を独自に構築し,それを材料とした個体,組織,細胞についての解析を進めてきた。その結果,性転換魚の生殖腺において,生殖幹細胞と未分化体細胞の存在および機能の一端が見えてきた。本稿ではこうした生殖腺の性的可塑性ついての研究内容とシングルセル解析の取り組みについて紹介する。(著者抄録)

  • 雌性先熟魚ホシササノハベラの逆方向性転換は体長に依存する

    吉原佑哉, TAPAS Chakraborty, 松山倫也, 長濱嘉孝, 長濱嘉孝, 太田耕平

    日本比較内分泌学会大会及びシンポジウムプログラム・講演要旨   47th   2023

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  • ホシササノハベラの社会性による性転換に伴う脳下垂体の遺伝子発現動態

    山本優芽, 花井匠磨, CHAKRABORTY Tapas, 豊田敦, 伊藤武彦, 松山倫也, 長濱嘉孝, 長濱嘉孝, 太田耕平

    日本比較内分泌学会大会及びシンポジウムプログラム・講演要旨   46th   2022

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  • 社会環境依存型の性転換における脳下垂体の関与

    山本優芽, 花井匠磨, TAPAS Chakraborty, 豊田敦, 伊藤武彦, 松山倫也, 長濱嘉孝, 長濱嘉孝, 太田耕平

    日本水産学会大会講演要旨集(CD-ROM)   2022   2022

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  • 海産魚における生殖幹細胞の長期培養法

    水村航大, TAPAS Chakraborty, 八尋逸清, 太田耕平, 長濱嘉孝, 長濱嘉孝, 松山倫也

    日本水産学会大会講演要旨集(CD-ROM)   2022   2022

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  • ホシササノハベラ生殖腺の性転換に伴う細胞増殖動態と血中ステロイドの分泌パターン

    花井匠磨, CHAKRABORTY Tapas, 松山倫也, 長濱嘉孝, 太田耕平

    日本水産学会九州支部総会・大会・例会講演要旨集   2021 (CD-ROM)   2022

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  • Genome Editing in Fish Reviewed

    Sipra Mohapatra, Tapas. Chakraborty

    Springer, Singapore   2021.8

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  • Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). Reviewed

    Autophagy   2021.2

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  • Aquaculture and stress management- a review of probiotic intervention. Reviewed

    S. Mohapatra, Tapas Chakraborty, V. Kumar, G. DeBoeck, K.N. Mohanta

    Journal of Animal Physiology and Animal Nutrition.   2013.5

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    Language：English   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

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Works

  • Conducted field/farm trials using malta oligo enriched feed

    Chakraborty Tapas

    2017 - Present

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  • Harmful algae and disease monitoring

    Chakraborty Tapas

    2017 - Present

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  • Continuous supply of marine fish seedlings for experimental and production purpose

    Chakraborty Tapas

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    Work type：Educational material  

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  • Establishing gonadal stem cell culture and propagation

    Chakraborty Tapas

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    Work type：Educational material  

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  • Developed the English website for the research station

    Chakraborty Tapas

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    Work type：Web Service  

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Industrial property rights

Patent   Number of applications: 1   Number of registrations: 0
Utility model   Number of applications: 0   Number of registrations: 0
Design   Number of applications: 0   Number of registrations: 0
Trademark   Number of applications: 0   Number of registrations: 0

Professional Memberships

  • Japanese society of fisheries science

    2020.10 - Present

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  • International Society for Fish Endocrinology

  • International Society of Stem cell Research

  • ZOOLOGICAL SOCIETY OF JAPAN

  • JAPANESE SOCIETY OF GENOME EDITING

  • Japanese Society of Fisheries Sciences

  • ZOOLOGICAL SOCIETY OF JAPAN

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  • JAPANESE SOCIETY OF GENOME EDITING

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  • Japanese Society of Fisheries Sciences

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  • International Society of Zoological Sciences (ISZS)

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  • International Society of Stem cell Research

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  • International Society for Stem Cell Research

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  • International Society for Fish Endocrinology (ISFE)

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  • International Society for Fish Endocrinology

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  • Indian Society of Zoological Sciences (ISZS)

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Committee Memberships

  • Local organizing member   3rd Ehime medaka forum  

    2017.4   

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    Committee type：Other

    Member

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  • Local organizing member   2nd Ehime medaka forum  

    2016   

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    Committee type：Other

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  • Editorial board member   Austin Journal of Endocrinology and Diabetes  

    - Present   

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    Committee type：Other

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  • Editorial board member   SM Journal of Endocrinology and Metabolism  

    - Present   

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    Committee type：Other

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  • Editorial board member   JSM Anatomy and Physiology  

    - Present   

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    Committee type：Other

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  • Editorial board member   Journal of Endocrine Disorders  

    - Present   

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    Committee type：Other

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Academic Activities

  • Screening of academic papers

    Role(s)： Peer review

    2024

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    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：100

    Proceedings of International Conference Number of peer-reviewed papers：3

  • Screening of academic papers

    Role(s)： Peer review

    2023

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    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：50

    Proceedings of International Conference Number of peer-reviewed papers：4

  • Ogranizing Secretary (international) International contribution

    Responsible Aquaculture and sustainable fisheries interect (RASHI) -2022  （ India India ） 2022.12

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    Type：Competition, symposium, etc. 

    Number of participants：1,000

  • Responsible Aquaculture and sustainable fisheries interect (RASHI) -2022 International contribution

    （ India India ） 2022.12

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    Type：Competition, symposium, etc. 

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  • Chairperson

    JSFS branch meeting, 2023  （ Online Japan ） 2022.1

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    Type：Competition, symposium, etc. 

  • JSFS branch meeting, 2023

    （ Online Japan ） 2022.1

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    Type：Competition, symposium, etc. 

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  • Frontiers in Molecular and Cellular Reproduction International contribution

    2022.1 - Present

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    Type：Academic society, research group, etc. 

  • Frontiers in Molecular and Cellular Reproduction International contribution

    2022.1

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    Type：Peer review 

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  • Screening of academic papers

    Role(s)： Peer review

    2022

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    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：15

  • Experimental Endocrinology International contribution

    2021.10 - Present

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    Type：Academic society, research group, etc. 

  • Experimental Endocrinology International contribution

    2021.10

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    Type：Peer review 

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  • Cells International contribution

    2021.5 - 2023.4

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    Type：Academic society, research group, etc. 

  • Cells International contribution

    2021.5 - 2023.4

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    Type：Peer review 

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  • Screening of academic papers

    Role(s)： Peer review

    2021

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    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：25

  • Screening of academic papers

    Role(s)： Peer review

    2019

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    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：10

    Number of peer-reviewed articles in Japanese journals：0

    Proceedings of International Conference Number of peer-reviewed papers：0

    Proceedings of domestic conference Number of peer-reviewed papers：0

  • Screening of academic papers

    Role(s)： Peer review

    2018

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    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：15

    Number of peer-reviewed articles in Japanese journals：0

    Proceedings of International Conference Number of peer-reviewed papers：0

    Proceedings of domestic conference Number of peer-reviewed papers：0

  • Conducted field/farm trials using malta oligo enriched feed

    Role(s)： Planning/Implementing academic research

    2017 - Present

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    Type：Academic research 

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  • Harmful algae and disease monitoring

    Role(s)： Planning/Implementing academic research

    2017 - Present

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    Type：Academic research 

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  • Screening of academic papers

    Role(s)： Peer review

    2017

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    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：5

    Number of peer-reviewed articles in Japanese journals：0

    Proceedings of International Conference Number of peer-reviewed papers：0

    Proceedings of domestic conference Number of peer-reviewed papers：0

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Research Projects

  • 「餌止め」はなぜ赤潮による魚類斃死を抑制するのか？

    2022.7 - 2023.4

    一般社団法人 ヤンマー資源循環支援機構 (Japan) 

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    Authorship：Coinvestigator(s) 

  • Construction of novel biomaterial production system using Japanese anchovy as an animal factory for medicine, healthcare, and sustainable food production

    Grant number：22K19211  2022.6 - 2024.3

    科学研究費助成事業  挑戦的研究(萌芽)

    Mohapatra Sipra, 太田 耕平, Chakraborty Tapas

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    Grant type：Scientific research funding

    世界の人口増加に伴い、高品質な食品に加え、医薬品、サプリメント、ワクチンなど需要も増大し、これらを持続的に供給するシステムが必要になると予測される。しかしながら、生物工場による物質生産は一般的にコストが高く、産業化されているものは限られている。本研究では、多産でライフサイクルの短いカタクチイワシを利用し、ゲノム編集によるノックイン法により、魚卵に有用物質を蓄積させ、これを餌とすることで養殖魚に機能性や高付加価値を付与する。本研究期間ではマダイイリドウイルスに対する「魚卵ワクチン」を生産して免疫付与の 効果を検証する。

    CiNii Research

  • 空間情報と1細胞解析の統合による魚類生殖腺の性的可塑性を制御する分子機構の解明

    2022.4 - 2026.3

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    Authorship：Coinvestigator(s) 

  • 魚類の幹細胞培養系とin vitro ゲノム編集による生殖幹細胞の未分化維持機構

    2022.4 - 2025.3

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    Authorship：Principal investigator 

  • 空間情報と 1 細胞解析の 統合による魚類生殖腺の 性的可塑性を制御する分 子機構の解明

    Grant number：22H00386  2022 - 2026

    日本学術振興会  科学研究費助成事業  基盤研究(A)

    太田 耕平, 荻野 由紀子, 馬場 崇, Chakraborty Tapas, Mohapatra Sipra

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    Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid)  Grant type：Scientific research funding

    魚類の性を自在に統御するには、それぞれの種が有する性的可塑性の特徴を細胞レベルで理解する必要がある。本研究では、性転換モデル系に空間的遺伝子発現解析を初めて導入し、生殖腺の性転換の起点になる未分化体細胞の位置情報をもとに生殖幹細胞との相互作用を分子レベルで明らかにする。同時に、各種シングルセル解析と培養系を駆使して研究を進め、“卵形成と精子形成の切り換え”の制御機構を解明する。さらに、雌雄異体魚との比較を行い、将来、全ての魚類において個体、生殖腺、および細胞の性を自在に統御するために必要な“性的可塑性の共通原理”に関する情報を得る。

    CiNii Research

  • 空間情報と1細胞解析の統合による魚類生殖腺の性的可塑性を制御する分子機構の解明

    2022 - 2025

    日本学術振興会  科学研究費助成事業  基盤研究(A)

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    Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid)  Grant type：Scientific research funding

  • Invitro genome editing in aquaculture

    Grant number：22K05832  2022 - 2024

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship：Principal investigator  Grant type：Scientific research funding

    CiNii Research

  • Construction of novel biomaterial production system using Japanese anchovy as an animal factory for medicine, healthcare, and sustainable food production

    2022 - 2023

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Challenging Research(Exploratory)

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    Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid)  Grant type：Scientific research funding

  • 近縁種免疫不全成魚へのクロマグロ生殖幹細胞移植による早期配偶子産生

    2020 - 2024

    戦略的創造研究推進事業 (文部科学省)

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    Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid)  Grant type：Contract research

  • 完全養殖マサバの生産拡 大と海外輸出のための戦 略的育種・生産基盤の開 発

    2020 - 2022

    戦略的創造研究推進事業 (文部科学省)

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    Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid)  Grant type：Contract research

  • in vitro genome editing of fish stem cell

    2020 - 2021

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    Authorship：Principal investigator  Grant type：On-campus funds, funds, etc.

  • Elucidation of molecular mechanism of sexual plasticity in fish gonad by cell analysis system and sex transformation

    2019.4 - 2022.3

    JSPS (Japan) 

    　 More details

    Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid) 

  • １細胞解析系と性転換モデルによる魚類生殖腺の性的可塑性に関する分子機構の解明

    Grant number：19H03049  2019 - 2022

    日本学術振興会  科学研究費助成事業  特別推進研究

    太田 耕平, 荻野 由紀子, Chakraborty Tapas, Mohapatra Sipra

    　 More details

    Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid)  Grant type：Scientific research funding

    魚類の性を自在に統御するには、それぞれの種が有する性的可塑性の特徴を細胞レベルで理解する必要がある。本研究では、研究代表者らは、自らが構築した性転換魚のモデル系を用い、生殖腺の性転換の起点となる2種の細胞集団(“生殖幹細胞”と“未分化体細胞”)に焦点を絞り、シングルセル解析系を導入して 生殖腺の転換の仕組みを解析する。さらに、雌雄異体魚との比較を行い、将来、全ての魚類において性を自在に統御するために必要な“細胞の共通原理”に関する情報を得る。

    CiNii Research

  • Elucidation of the molecular mechanism of autophagy on fertilization of fish

    2018.10 - 2021.3

    Sumitomo Foundation (Japan) 

    　 More details

    Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid) 

  • オートファジーが魚類の受精におよぼす分子機構の解明 [Elucidation of molecular mechanism of autophagy association in fish fertility]

    2018 - 2020

    Sumitomo Zaidan

    　 More details

    Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid)  Grant type：Contract research

  • Development of new breeding technologies for faster production of improved varieties from natural Yellow tail

    2016.10 - 2019.3

    Ministry of Agriculture, Forestry and Fisheries (Japan) 

    　 More details

    Authorship：Coinvestigator(s) 

  • Somatic cell epigenetics and gonadal sexual plasticity in fish

    2016.4 - 2018.3

    JSPS (Japan) 

    　 More details

    Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid) 

  • 魚類の生殖腺体細胞の性的可塑性を司るエピジェネティクスの分子基盤

    Grant number：16H04981  2016 - 2018

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    　 More details

    Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid)  Grant type：Scientific research funding

▼display all

Class subject

  • Special Lecture on Animal and Marine Science 2

    2024.12 - 2025.2   Winter quarter

  • 動物・海洋生物科学プロジェクト演習

    2024.10 - 2025.3   Second semester

  • 動物・海洋生物科学演習第二

    2024.10 - 2025.3   Second semester

  • 動物・海洋生物科学演習第一

    2024.10 - 2025.3   Second semester

  • 動物海洋生物科学ティーチング演習

    2024.4 - 2025.3   Full year

  • 国際交流演習Ⅰ

    2024.4 - 2025.3   Full year

  • プロジェクト演習

    2024.4 - 2025.3   Full year

  • インターンシップ

    2024.4 - 2025.3   Full year

  • ティーチング演習

    2024.4 - 2025.3   Full year

  • 動物・海洋生物科学特別実験

    2024.4 - 2025.3   Full year

  • 022年度前期・他・水産科学のフロンティア

    2024.4 - 2024.9   First semester

  • 国際演示技法

    2024.4 - 2024.9   First semester

  • 演示技法Ⅰ

    2024.4 - 2024.9   First semester

  • 動物・海洋生物科学特別研究第二

    2024.4 - 2024.9   First semester

  • 動物・海洋生物科学特別研究第一

    2024.4 - 2024.9   First semester

  • 動物・海洋生物科学特別研究第一

    2024.4 - 2024.9   First semester

  • Special Lecture on Advanced Topics of Agriculture 4

    2023.12 - 2024.2   Winter quarter

  • 動物・海洋生物科学プロジェクト演習

    2023.10 - 2024.3   Second semester

  • 動物・海洋生物科学演習第二

    2023.10 - 2024.3   Second semester

  • 動物・海洋生物科学演習第一

    2023.10 - 2024.3   Second semester

  • Special Lecture on Animal and Marine Science 2

    2023.10 - 2023.12   Fall quarter

  • 国際交流演習Ⅰ

    2023.4 - 2024.3   Full year

  • 動物海洋生物科学ティーチング演習

    2023.4 - 2024.3   Full year

  • 動物・海洋生物科学特別実験

    2023.4 - 2024.3   Full year

  • ティーチング演習

    2023.4 - 2024.3   Full year

  • インターンシップ

    2023.4 - 2024.3   Full year

  • プロジェクト演習

    2023.4 - 2024.3   Full year

  • 国際演示技法

    2023.4 - 2023.9   First semester

  • 022年度前期・他・水産科学のフロンティア

    2023.4 - 2023.9   First semester

  • 動物・海洋生物科学特別研究第一

    2023.4 - 2023.9   First semester

  • 動物・海洋生物科学特別研究第一

    2023.4 - 2023.9   First semester

  • 動物・海洋生物科学特別研究第二

    2023.4 - 2023.9   First semester

  • 演示技法Ⅰ

    2023.4 - 2023.9   First semester

  • Special lecture on Advanced Topics of Agriculture

    2022.10 - 2023.3   Second semester

  • 動物・海洋生物科学プロジェクト演習

    2022.10 - 2023.3   Second semester

  • 動物・海洋生物科学演習第一

    2022.10 - 2023.3   Second semester

  • 動物・海洋生物科学演習第二

    2022.10 - 2023.3   Second semester

  • 動物海洋生物科学ティーチング演習

    2022.4 - 2023.3   Full year

  • Master’s Thesis Research II

    2022.4 - 2023.3   Full year

  • Master’s Thesis Research I

    2022.4 - 2023.3   Full year

  • Internship

    2022.4 - 2023.3   Full year

  • International presentation skill for academic meeting

    2022.4 - 2023.3   Full year

  • 動物・海洋生物科学特別演習

    2022.4 - 2023.3   Full year

  • 動物・海洋生物科学特別講究

    2022.4 - 2023.3   Full year

  • 国際交流演習Ⅳ

    2022.4 - 2023.3   Full year

  • 国際交流演習Ⅲ

    2022.4 - 2023.3   Full year

  • 国際交流演習Ⅱ

    2022.4 - 2023.3   Full year

  • 国際交流演習Ⅰ

    2022.4 - 2023.3   Full year

  • プロジェクト演習

    2022.4 - 2023.3   Full year

  • インターンシップ

    2022.4 - 2023.3   Full year

  • 国際演示技法

    2022.4 - 2023.3   Full year

  • 演示技法Ⅰ

    2022.4 - 2023.3   Full year

  • ティーチング演習

    2022.4 - 2023.3   Full year

  • 動物・海洋生物科学特別実験

    2022.4 - 2023.3   Full year

  • 2022年度前期・他・水産科学のフロンティア

    2022.4 - 2022.9   First semester

  • Advanced Animal & Marine Biosciences

    2022.4 - 2022.9   First semester

  • Presentation skill for academic meeting Ⅰ

    2022.4 - 2022.9   First semester

  • Teaching Practice

    2022.4 - 2022.9   First semester

  • Seminar in a Specified Field Ⅱ

    2022.4 - 2022.9   First semester

  • Seminar in a Specified Field Ⅰ

    2022.4 - 2022.9   First semester

  • 動物・海洋生物科学特別研究第二

    2022.4 - 2022.9   First semester

  • 動物・海洋生物科学特別研究第一

    2022.4 - 2022.9   First semester

  • 動物・海洋生物科学特別研究第一

    2022.4 - 2022.9   First semester

  • Special lecture on Advanced Topics of Agriculture

    2021.10 - 2022.3   Second semester

  • 動物・海洋生物科学演習第二

    2021.10 - 2022.3   Second semester

  • 動物・海洋生物科学プロジェクト演習

    2021.10 - 2022.3   Second semester

  • 動物・海洋生物科学演習第一

    2021.10 - 2022.3   Second semester

  • Master’s Thesis Research II

    2021.4 - 2022.3   Full year

  • 動物・海洋生物科学特別演習

    2021.4 - 2022.3   Full year

  • International presentation skill for academic meeting

    2021.4 - 2022.3   Full year

  • Internship

    2021.4 - 2022.3   Full year

  • Master’s Thesis Research I

    2021.4 - 2022.3   Full year

  • 動物・海洋生物科学特別講究

    2021.4 - 2022.3   Full year

  • 動物海洋生物科学ティーチング演習

    2021.4 - 2022.3   Full year

  • 動物海洋生物科学ティーチング演習

    2021.4 - 2022.3   Full year

  • 演示技法Ⅰ

    2021.4 - 2022.3   Full year

  • 演示技法Ⅱ

    2021.4 - 2022.3   Full year

  • 国際演示技法

    2021.4 - 2022.3   Full year

  • 国際交流演習Ⅱ

    2021.4 - 2022.3   Full year

  • 国際交流演習Ⅲ

    2021.4 - 2022.3   Full year

  • 国際交流演習Ⅳ

    2021.4 - 2022.3   Full year

  • 動物・海洋生物科学特別実験

    2021.4 - 2022.3   Full year

  • ティーチング演習

    2021.4 - 2022.3   Full year

  • 演示技法Ⅰ

    2021.4 - 2022.3   Full year

  • 国際演示技法

    2021.4 - 2022.3   Full year

  • インターンシップ

    2021.4 - 2022.3   Full year

  • プロジェクト演習

    2021.4 - 2022.3   Full year

  • 国際交流演習Ⅰ

    2021.4 - 2022.3   Full year

  • 国際交流演習Ⅱ

    2021.4 - 2022.3   Full year

  • 国際交流演習Ⅲ

    2021.4 - 2022.3   Full year

  • 国際交流演習Ⅳ

    2021.4 - 2022.3   Full year

  • Advanced Animal & Marine Biosciences

    2021.4 - 2021.9   First semester

  • Seminar in a Specified Field Ⅰ

    2021.4 - 2021.9   First semester

  • Seminar in a Specified Field Ⅱ

    2021.4 - 2021.9   First semester

  • Teaching Practice

    2021.4 - 2021.9   First semester

  • Presentation skill for academic meeting Ⅰ

    2021.4 - 2021.9   First semester

  • 動物・海洋生物科学特別研究第二

    2021.4 - 2021.9   First semester

  • 動物・海洋生物科学特別研究第一

    2021.4 - 2021.9   First semester

  • 2020年度前期集中・他・水産生物学実験第一

    2020.4 - 2020.9   First semester

  • Special Lecture on Animal and Marine Science 2

    2024.12 - 2025.2   Winter quarter

▼display all

FD Participation

  • 2024.5   Title：令和６年第３回全学FD 「学内で簡単に被験者実験のモニタを勧誘・謝礼支払いができる学内リサーチコラボレータシステムについて」）

    Organizer：University-wide

  • 2024.5   Title：令和６年度全学FD 「自殺防止メンタルヘルス研修会」について

    Organizer：University-wide

  • 2024.4   Role：Other   Title：令和６年度 第１回全学FD（新任教員の研修）The 1st All-University FD (training for new faculty members) in FY2024

    Organizer：University-wide

  • 2024.4   Role：Participation   Title：令和３年度 第１回全学FD（新任教員の研修）The 1st All-University FD (training for new faculty members) in FY2021

    Organizer：University-wide

  • 2022.6   Role：Participation   Title：How to Maintain Mental Health (during a pandemic)?: Lessons from Applied Psychology

    Organizer：University-wide

  • 2022.4   Role：Speech   Title：The 1st All-University FD (training for new faculty members) in FY2022

    Organizer：University-wide

  • 2022.2   Role：Participation   Title：高等教育国際シンポジウム「ウィズコロナ時代に高等教育は何を保証するのか」開催案内

  • 2021.12   Role：Participation   Title：Deciding the Future of Kyushu University

    Organizer：University-wide

  • 2021.11   Role：Participation   Title：農学研究院FD「人を対象とする生命科学・医学系研究に関する倫理指針」について

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2021.9   Role：Participation   Title：Financial Planning in Japan: Medical System, Insurance and Loans

  • 2021.7   Role：Participation   Title：What is COIL? Examples of implementation by the School of Agriculture & the School of Interdisciplinary Science and Innovation

    Organizer：University-wide

  • 2021.7   Role：Participation   Title：農学研究院FD「科研費を獲りにいこう！　科研費獲得の技術と工夫」

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2021.3   Role：Participation   Title：オンライン授業におけるアクティブラーニングの科目の実施状況：アクティブラーニングの今後を考える

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2021.3   Role：Participation   Title：アドミッション・スペシャリスト能力開発研修会（第5回）

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2020.12   Role：Participation   Title：【オンライン配信】新型コロナウィルス感染拡大状況での学生のメンタルヘルス

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2020.12   Role：Participation   Title：大学の研究評価の現状と農学研究院の「部局独自の評価基準」案における業績分析

    Organizer：[Undergraduate school/graduate school/graduate faculty]

  • 2020.9   Role：Participation   Title：科研費を獲りにいこう！　勝ち抜く気合と技術

    Organizer：[Undergraduate school/graduate school/graduate faculty]

▼display all

Other educational activity and Special note

  • 2022  Special Affairs 

  • 2021  Special Affairs 

Outline of Social Contribution and International Cooperation activities

  • I am working with a goal to contribute to both scientific and local fish farmer societies to uplift fish production, enhance knowledge transparency and create a sustainable society.

Social Activities

  • faculty foreign training sponsored by the Indian Council of Agricultural Research, New Delhi, India through the university IDP-NAHEP project

    2023.9 - 2023.10

    　More details

    Type：Other

    researchmap

  • Vice president of COFLAA, India

    2023

    　More details

    Vice president of COFLAA, India

  • MOU with Polytechnic University of Marche, Italy.

    2023

    　More details

    MOU with Polytechnic University of Marche, Italy.

  • MOU with Polytechnic University of Marche, Italy.

    2023

    　More details

    Type：Other

    researchmap

  • Vice president of COFLAA, India

    2023

    　More details

    Type：Other

    researchmap

  • faculty foreign training sponsored by the Indian Council of Agricultural Research, New Delhi, India through the university IDP-NAHEP project

    2022.8 - 2022.11

    　More details

    Type：Other

    researchmap

  • Foreign co-ordinator of COFLAA, India.

    2021

    　More details

    Foreign co-ordinator of COFLAA, India.

  • Foreign co-ordinator of COFLAA, India.

    2021

    　More details

    Type：Other

    researchmap

  • Disseminated ideas about fish and fish culture to the high school students

    Minamiuwa high school, Ehime Japan  2019.8

    　More details

    Audience： Infants,　Schoolchildren,　Junior students,　High school students

    Type：Seminar, workshop

  • Disseminated ideas about fish and fish culture to the high school students

    Minamiuwa high school, Ehime Japan  2019.8

    　More details

    Type：Seminar, workshop

    researchmap

  • Taught english to the primary and high school teachers of Ainan town

    Ainan Town office  Ainan, Ehime, Japan  2018.3

    　More details

    Audience： General,　Scientific,　Company,　Civic organization,　Governmental agency

    Type：Other

  • Taught english to the primary and high school teachers of Ainan town

    Ainan Town office  Ainan, Ehime, Japan  2018.3

    　More details

    Type：Other

    researchmap

  • organized training (volunteer) for Primary and high school teachers

    2018

    　More details

    organized training (volunteer) for Primary and high school teachers

  • organized training (volunteer) for Primary and high school teachers

    2018

    　More details

    Type：Other

    researchmap

  • India and Japan: differences and commonalities.

    Minamiuwa high school, Ehime Japan  2017.8

    　More details

    Audience： Infants,　Schoolchildren,　Junior students,　High school students

    Type：Seminar, workshop

  • India and Japan: differences and commonalities.

    Minamiuwa high school, Ehime Japan  2017.8

    　More details

    Type：Seminar, workshop

    researchmap

  • Talk on International researcher experience

    Role(s)： Appearance

    　More details

    Type：Research consultation

    researchmap

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Media Coverage

  • The programme was done to showcase the work being done at the SEFRC, and the life and work of the foreign nationals staying there. TV or radio program

    RNB -cooperation  2019.3

    　More details

    The programme was done to showcase the work being done at the SEFRC, and the life and work of the foreign nationals staying there.

  • Talk on International researcher experience

    National institute for basic biology (NIBB, Japan)  2012.3

    　More details

    Talk on International researcher experience

  • Aquaculture in shikoku TV or radio program

    　More details

  • Tuna and related species aquaculture innovation in japan TV or radio program

    　More details

  • Fisheries development TV or radio program

    　More details

Educational Activities for Highly-Specialized Professionals in Other Countries

  • 2023.9 - 2023.10   faculty foreign training sponsored by the Indian Council of Agricultural Research, New Delhi, India through the university IDP-NAHEP project

    Main countries of student/trainee affiliation：India

    Other countries of student/trainee affiliation：India

  • 2022.8 - 2022.11   faculty foreign training sponsored by the Indian Council of Agricultural Research, New Delhi, India through the university IDP-NAHEP project

    Main countries of student/trainee affiliation：Japan

    Other countries of student/trainee affiliation：India

Acceptance of Foreign Researchers, etc.

  • CIFE

    Acceptance period： 2023.10 - 2023.11   （Period）：1 month or more

    Nationality：India

    Business entity：Foreign governments, foreign research institutes, international organizations

  • CIFE

    Acceptance period： 2023.10 - 2023.11   （Period）：1 month or more

    Nationality：India

    Business entity：Foreign governments, foreign research institutes, international organizations

  • CIFE

    Acceptance period： 2023.9 - 2023.10   （Period）：1 month or more

    Nationality：India

    Business entity：Foreign governments, foreign research institutes, international organizations

  • CIFE

    Acceptance period： 2023.9 - 2023.10   （Period）：1 month or more

    Nationality：India

    Business entity：Foreign governments, foreign research institutes, international organizations

  • SEFDEC

    Acceptance period： 2023.9 - 2023.10   （Period）：Less than 2 weeks

    Nationality：Malaysia

    Business entity：Foreign governments, foreign research institutes, international organizations

  • CIFE

    Acceptance period： 2023.9 - 2023.10   （Period）：1 month or more

    Nationality：India

    Business entity：Foreign governments, foreign research institutes, international organizations

  • CIFE

    Acceptance period： 2023.9 - 2023.10   （Period）：1 month or more

    Nationality：India

    Business entity：Foreign governments, foreign research institutes, international organizations

  • CIFE

    Acceptance period： 2023.9 - 2023.10   （Period）：1 month or more

    Nationality：India

    Business entity：Foreign governments, foreign research institutes, international organizations

  • CIFE

    Acceptance period： 2023.9 - 2023.10   （Period）：1 month or more

    Nationality：India

    Business entity：Foreign governments, foreign research institutes, international organizations

  • Central Agricultural university

    Acceptance period： 2022.8 - 2022.10   （Period）：1 month or more

    Nationality：India

    Business entity：Foreign governments, foreign research institutes, international organizations

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Travel Abroad

  • 2023.12

    Staying countory name 1：India   Staying institution name 1：Central agricultural university

  • 2023.6

    Staying countory name 1：Greece   Staying institution name 1：HCMR

  • 2023.5 - 2024.5

    Staying countory name 1：Greece   Staying institution name 1：Institute of Marine Biology, Biotechnology & Aquaculture Hellenic Center for Marine Research

  • 2022.7 - 2023.7

    Staying countory name 1：Portugal   Staying institution name 1：CCMAR, Protugal

  • 2019.11 - 2019.12

    Staying countory name 1：China   Staying institution name 1：Southwest university, China

    Staying countory name 2：India   Staying institution name 2：Central agricultural university, India

  • 2019.11

    Staying countory name 1：India   Staying institution name 1：Central agricultural university, India

  • 2018.6

    Staying countory name 1：Brazil   Staying institution name 1：NA

  • 2017.6

    Staying countory name 1：Canada   Staying institution name 1：NA

  • 2016.6

    Staying countory name 1：Sweden   Staying institution name 1：NA

  • 2014.6

    Staying countory name 1：Portugal   Staying institution name 1：NA

▼display all