Updated on 2025/06/16

Information

 

写真a

 
WATANABE ATSUSHI
 
Organization
Faculty of Agriculture Department of Agro-environmental Sciences Professor
Graduate School of Bioresource and Bioenvironmental Sciences Department of Agro-environmental Sciences（Concurrent）
School of Agriculture Department of Bioresource and Bioenvironment（Concurrent）
Title
Professor
Contact information
メールアドレス
Tel
0928024646
Profile
令和6年度の教育活動のうち、学部講義では、前期（春・夏学期）に造林学（主・分担）・植物代謝制御学（副・分担）・森林植物学実験（副・分担）、後期（秋学期）には、森林植物学（主・分担）を担当した。大学院では、春学期に森林・林業の科学（副・分担）、森林資源管理学（副・分担）、秋学期は造林学特論（主）を担当した。基幹教育院については、全学教育として農学入門（通年）を担当し、農学部国際コースの専攻教育科目講義「Forest Science Products」（前期・主・分担）について担当した。研究室の教育・研究活動として、造林学研究室の博士後期課程2名、修士課程6名、学部4年生3名および学部３年生3名の研究と教育の指導を行った。 研究活動では、久留米市と連携してくるめつつじのDNA分析調査・九州スギ起源解明・マツノザイセンチュウ薬剤抵抗性および樹木根に関する研究を行った。科学研究補助金基盤研究として、分担として科研Bとしてマツ材線虫病の萎凋枯死メカニズムの全容解明および災害後の森林環境と人間の関係性を再生する芸術文化的実践―英彦山分水嶺を中心に―に従事した。その他民間企業との共同研究に従事した。
External link

Research Areas

  • Environmental Science/Agriculture Science / Science in plant genetics and breeding

  • Environmental Science/Agriculture Science / Plant protection science

  • Environmental Science/Agriculture Science / Conservation of biological resources

  • Environmental Science/Agriculture Science / Horticultural science

Degree

  • Agricultural Science （ 1999.3 Kyushu University ）

Research History

  • Kyushu University Faculty of Agriculture Department of Agro-environmental Sciences  Professor 

    2012.8 - Present

  • 森林総合研究所林木育種センター 育種第１課基盤技術研究室 研究室長 

    2008.4 - 2012.7

  • 森林総合研究所林木育種センター 育種部育種第２課育種研究室 研究室長 

    2006.8 - 2008.3

  • 森林総合研究所林木育種センター 育種部先端研究室 研究員 

    2006.4 - 2006.7

  • 独立行政法人林木育種センター 育種工学課QTL研究室 研究員 

    2002.4 - 2006.3

  • Kyushu University  平成11年度日本学術振興会特別研究員（PD） 

    1999.4 - 2002.3

  • Kyushu University  平成８年度日本学術振興会特別研究員（DC2） 

    1996.4 - 1998.3

  • なし   

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Education

  • Kyushu University   農学部   林産学専攻

    1995.4 - 1999.3

Research Interests・Research Keywords

  • Research theme： Elucidation of rooting induction signals and root system formation process in Cryptomeria japonica.

    Keyword： Cryptomeria japonica, cutting, rooting, signal

    Research period： 2018.4 - Present

  • Research theme： Molecular genetics and gene expression profiling associated with Pine Wilt Disease toward protection of Pine forest in Japan

    Keyword： pathogen related gene, gene expression profiling, field phenotype, gene diversity, database, next generation resistant breeding

    Research period： 2012.8 - Present

  • Research theme： Identification of Rhododendron cultivar using DNA barcoding

    Keyword： Rhododendron・DNA barcoding/ culativar

    Research period： 2013.10 - Present

  • Research theme： Historical verification of the origin of Kyushu Sugi cedar(Cryptomeria japonica) and the establishment of native varieties.

    Keyword： Cryptomeria japonica/cultivar/kyushu region

    Research period： 2023.4 - Present

  • Research theme： Selection of Toxicodendron species which quantity of sap flow is improved using Molecular techniques

    Keyword： Toxicodendron sp, tree breeding, molecular marker, gene expression

    Research period： 2015.4 - Present

  • Research theme： Forest management using DNA marker and GIS

    Keyword： Genetic resources in Forest, gene diversity

    Research period： 2013.4

  • Research theme： Several studies associated with Forest Tree Breeding in Japan

    Keyword： root cutting, stress responsibility, wood property, pollen allergy, phenotype, lineage management, database

    Research period： 2012.8

Awards

  • the Best Poster Award

    2019.10   IUFRO  

  • 日本森林学会論文賞

    2017.3   日本森林学会  

  • フジサンケイビジネスアイ賞

    2012.9   自動総合認識展  

  • 日本木材学会論文賞

    2012.3   日本木材学会  

  • 第51回林木育種賞受賞

    2008.5   林木育種協会  

Papers

  • “九州本土の天然杉”とされる大崩山系鬼の目山スギ個体群の遺伝解析に基づく遺存集団としての可能性の検証

    弓削直樹・田村美帆・武津英太郎・渡辺敦史

    日本森林学会誌   2025.3

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    Authorship：Corresponding author  

    鬼の目山のスギ個体群の遺伝構造は、本州・屋久島および九州内に残るスギ古木のいずれからも特異であり、これは氷河期などに小集団化し、隔離分布した結果であることを示すと考えられた。この結果をもとに、鬼の目山のスギ個体群は氷河期から遺存する個体群であり、九州本土で唯一天然スギとして現在まで遺存した個体群の可能性を示唆している。

  • 雄性不稔スギ「爽春」の雄性不稔原因遺伝子を持つ個体を検出する簡易DNAマーカー の開発 Reviewed

    坪村美代子, 郷田乃真人, 平尾知士, 三嶋賢太郎, 小長谷賢一, 田村美帆, 高橋誠, 渡辺敦史

    日本森林学会誌   101 ( 4 )   155 - 162   2019.8

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    Development of simple DNA markers for selecting trees with the male-sterile gene of Cryptomeria japonica “Sosyun”
    We report the development of two types of simple DNA markers that can be used to easily identify genotypes of both the male-sterile and wild-type individuals at a locus, which is tightly linked to the causative gene of male sterility in the “Sosyun” clone of Cryptomeria japonica. Both the allele-specific primer (ASP) PCR marker and cleaved amplified polymorphic sequence (CAPS) marker were designed using previously reported single nucleotide polymorphism (SNP) information. The cDNA and genomic DNA of the EST reCj19250, which is linked to the ms-1 locus, were sequenced for both the “Sosyun” and wild-type plus-tree “Usui2” clones. Primers were designed for detecting both the sterile and wild-type SNPs using the genomic DNA sequences. For the ASP-PCR marker, homozygous recessive male-sterile individuals yielded a single 157-bp fragment, whereas the homozygous wild-type individuals yielded a single 345-bp fragment, and heterozygotes yielded both fragments. Similarly, for the CAPS marker, male-sterile individuals yielded a single 101-bp fragment after digestion, whereas wild-type ones yielded an intact single 125-bp fragment, and heterozygotes yielded fragments of both sizes. These markers could be utilized for identifying male-sterile individuals during seedling production and for identifying heterozygous trees for future tree breeding.

    DOI： 10.4005/jjfs.101.155

  • Historical seed use and transfer affects geographic specificity in genetic diversity and structure of old planted Pinus thunbergii populations Reviewed

    Masakazu G. Iwaizumi, Shousuke Miyata, Tomonori Hirao, Miho Tamura, Atsushi Watanabe

    Forest Ecology and Management   408   211 - 219   2018.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    Although most molecular ecology studies examining genetic variation have focused on natural forests, for some major tree species, natural forests are nearly extinct, and the remaining genetic resources are mainly planted forests. In order to manage the genetic variability and develop a conservation strategy for such species, it is important to examine genetic variation resulting from historical processes during repeated artificial population development through plantations. The geographic pattern of genetic diversity and structure of 49 old planted Pinus thunbergii populations (2755 trees) distributed across Japan was examined using seven nuclear microsatellite markers. We found that allelic diversity was generally lower in both northern and eastern populations; however, locally, some populations in other regions also exhibited low allelic diversity. The overall value of the standardized measure of population differentiation (G′ST = 0.206) was higher than that of both other widespread Japanese conifers and continental Pinus species. STRUCTURE software revealed a general gradual cline in genetic structure from southwestern to northeastern populations; however, some populations on the Japan Sea side showed quite a different local proportion of cluster memberships from nearby populations. These observations indicated that most of the preserved, planted populations of P. thunbergii possess regional genetic variation, but some populations were developed from seed pools derived from other regions, possibly through distribution by ship along the Japan Sea. Information on this specific genetic variation as a result of historical seed use and transfer should assist the design of several conservation units and breeding zones, while also taking care of a deep-seated need for conservation of pine forests by local people.

    DOI： 10.1016/j.foreco.2017.10.026

  • Spatiotemporal analysis of pine wilt disease Relationship between pinewood nematode distribution and defence response in Pinus thunbergii seedlings Reviewed

    Rimi Yamaguchi, Koji Matsunaga, Tomonori Hirao, Miho Tamura, Atsushi Watanabe

    Forest Pathology   2019.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    Pine wilt disease is of major concern as it has destroyed pine forests in East Asia and Europe. Several studies have suggested that invasion by the pinewood nematode (PWN) Bursaphelenchus xylophilus, which causes this disease, evokes an excessive defence response in pine trees, resulting in tree death. However, few studies have quantitatively evaluated the correlation between PWN distribution and tree defence responses. Therefore, the present study aimed to quantify the number of PWNs and expression levels of putative pathogenesis-related (PR) genes in different positions of Japanese black pine (Pinus thunbergii) seedlings over time. To quantify the number of PWNs in the seedlings, we used TaqMan quantitative real-time PCR (qPCR) assay. During the early phase of infection, most PWNs were distributed around the inoculated sites, with only a small number being detected at distant sites, but the expression levels of PR genes were highly upregulated throughout the seedlings. Both the number of PWNs and expression levels of PR genes then increased drastically throughout the seedlings, all of which exhibited external symptoms. Thus, it appears that the rapid migration of PWNs induces a defence response throughout the seedling; however, this may not be effective in controlling these parasites, thereby ultimately leading to plant death.

    DOI： 10.1111/efp.12518

  • Clock genes and diurnal transcriptome dynamics in summer and winter in the gymnosperm Japanese cedar (Cryptomeria japonica (L.f.) D.Don) Reviewed International journal

    Nose Mine, 渡辺敦史

    BMC PLANT BIOLOGY   14   2014.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1186/s12870-014-0308-1

  • Construction of a core collection and evaluation of genetic resources for Cryptomeria japonica (Japanese cedar) Reviewed International journal

    Miyamoto, Naoko, Ono, Masako, 渡辺敦史

    JOURNAL OF FOREST RESEARCH   20 ( 1 )   2015.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s10310-014-0460-3

  • Determination of male strobilus developmental stages by cytological and gene expression analyses in Japanese cedar (Cryptomeria japonica) Invited Reviewed International journal

    坪村美代子, 栗田学, 渡辺敦史

    TREE PHYSIOLOGY   36 ( 5 )   653 - 666   2016.5

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/treephys/tpw001

  • Demonstration of Genome-Wide Association Studies for Identifying Markers for Wood Property and Male Strobili Traits in Cryptomeria japonica Reviewed International journal

    Uchiyama, Kentaro, Iwata, Hiroyoshi, Moriguchi, Yoshinari, Ujino-Ihara, Tokuko, Ueno, Saneyoshi, Taguchi, Yuriko, Tsubomura, Miyoko, Mishima, Kentaro, Iki, Taiichi, 渡辺敦史, Futamura, Norihiro, Shinohara, Kenji, Tsumura, Yoshihiko

    PLOS ONE   8 ( 11 )   e79866   2013.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1371/journal.pone.0079866

  • Changes in annual transcriptome dynamics of a clone of Japanese cedar (Cryptomeria japonica D. Don) planted under different climate conditions. International journal

    Mine Nose, So Hanaoka, Eitaro Fukatsu, Manabu Kurita, Masahiro Miura, Yuichiro Hiraoka, Taiichi Iki, Osamu Chigira, Kentaro Mishima, Makoto Takahashi, Atsushi Watanabe

    PloS one   18 ( 2 )   e0277797   2023.2   （ ISSN:1932-6203 ）

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：PLoS ONE  

    Environmental responses are critical for plant growth and survival under different climate conditions. To elucidate the underlying biological mechanisms of environmental responses in Japanese cedar (Cryptomeria japonica D. Don), the annual transcriptome dynamics of common clonal trees (Godai1) planted at three different climate sites (Yamagata, Ibaraki, and Kumamoto Prefectures) were analyzed using microarrays. Both principal component analysis (PCA) and hierarchical clustering of the microarray data indicated the transition to dormant transcriptome status occurred earlier and the transition to active growth status later in the colder region. Interestingly, PCA also indicated that the transcriptomes of trees grown under three different conditions were similar during the growth period (June to September), whereas the transcriptomes differed between sites during the dormant period (January to March). In between-site comparisons, analyses of the annual expression profiles of genes for sites 'Yamagata vs. Kumamoto', 'Yamagata vs. Ibaraki', and 'Ibaraki vs. Kumamoto' identified 1,473, 1,137, and 925 targets exhibiting significantly different expression patterns, respectively. The total of 2,505 targets that exhibited significantly different expression patterns in all three comparisons may play important roles in enabling cuttings to adapt to local environmental conditions. Partial least-squares regression analysis and Pearson correlation coefficient analysis revealed that air temperature and day length were the dominant factors controlling the expression levels of these targets. GO and Pfam enrichment analyses indicated that these targets include genes that may contribute to environmental adaptation, such as genes related to stress and abiotic stimulus responses. This study provided fundamental information regarding transcripts that may play an important role in adaptation to environmental conditions at different planting sites.

    DOI： 10.1371/journal.pone.0277797

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  • Identification of novel putative causative genes and genetic marker for male sterility in Japanese cedar (Cryptomeria japonica D.Don) Reviewed

    Kentaro Mishima, Tomonori Hirao, Miyoko Tsubomura, Miho Tamura, Manabu Kurita, Mine Nose, So Hanaoka, Makoto Takahashi, Atsushi Watanabe

    BMC Genomics   19 ( 1 )   2018.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    Background: Japanese cedar (Cryptomeria japonica) is an important tree for Japanese forestry. Male-sterile marker development in Japanese cedar would facilitate selection of male-sterile plus trees, addressing the widespread social problem of pollinosis and facilitating the identification of heterozygotes, which are useful for breeding. Results: This study used next-generation sequencing for single-nucleotide polymorphism discovery in libraries constructed from several organs, including male-sterile and male-fertile strobili. The single-nucleotide polymorphisms obtained were used to construct a high-density linkage map, which enabled identification of a locus on linkage group 9 strongly correlated with male-sterile trait. Expressed sequence tags corresponding to 11 marker loci from 5 isotigs were associated with this locus within 33.4-34.5 cM. These marker loci explained 100% of the phenotypic variation. Several homologs of these sequences are associated with male sterility in rice or Arabidopsis, including a pre-mRNA splicing factor, a DEAD-box protein, a glycosyl hydrolase, and a galactosyltransferase. These proteins are thus candidates for the causal male-sterile gene at the ms-1 locus. After we used a SNaPshot assay to develop markers for marker-assisted selection (MAS), we tested F2 progeny between male-sterile and wild-type plus trees to validate the markers and extrapolated the testing to a larger plus-tree population. We found that two developed from one of the candidates for the causal gene were suitable for MAS. Conclusions: More than half of the ESTs and SNPs we collected were new, enlarging the genomic basis for genetic research on Japanese cedar. We developed two SNP markers aimed at MAS that distinguished individuals carrying the male-sterile trait with 100% accuracy, as well as individuals heterozygous at the male-sterile locus, even outside the mapping population. These markers should enable practical MAS for conifer breeding.

    DOI： 10.1186/s12864-018-4581-5

  • The Difference of the Thoughts on the Planting of Japanese Cedar Trees from the Viewpoints on the Origin of Old Japanese Cedar in Kirishima Jingū Shrine and Hikosan Jingū Shrine

    WATANABE Atsushi

    Japanese mountain religion   74   73 - 88   2024.11   （ ISSN:09150978 ）

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    Language：Japanese   Publisher：日本山岳修験学会  

    はじめに / 一、生物進化の中で生物は地域で固有のDNA型を示す / 二、九州のスギは本州からの人為植栽なのか？ / 三、さし木と九州地域のさし木林業 / 四、鬼の目山のスギと九州スギ遺存説 / 五、DNA分析による九州スギ古木のさし木による植栽の証拠 / 六、霧島神宮とメアサ / 七、御神木の起源を求めて / 八、英彦山神宮とホンスギ / おわりに

    CiNii Research

  • Reforestation or Genetic Disturbance: A Case Study of Pinus thunbergii in the Iki-no-Matsubara Coastal Forest (Japan) Invited Reviewed International journal

    #Mukasyaf, Aziz Akbar; @Matsunaga, Koji; Tamura, Miho; @Iki, Taiichi; Watanabe, Atsushi; @Iwaizumi, Masakazu G.

    FORESTS   12 ( 1 )   2021.1

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    有名クロマツ林を対象に遺伝構造を明らかにし、クロマツ林成立過程を種苗の移動も含めて解明した。

    DOI： 10.3390/f12010072

  • 雄性不稔スギ爽春の原因遺伝子候補の特徴と花粉形成期に認められた糖代謝異常 Reviewed

    #Nanako Yamasaki, Taisei Horiba, Miho Tamura, @Noritsugu Kuramoto , Kuniyoshi　Shimizu, Atsushi Watanabe

    日本森林学会誌   102 ( 3 )   2020.6

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • 用土を用いない新たなスギ挿し木発根手法の検討 ―スギ挿し木苗の植物工場的生産技術の開発に向けて― Reviewed

    栗田 学・倉本哲嗣・久保田正裕・福山友博・竹田宣明・倉原雄二・ 松永孝治・大塚次郎・佐藤省治・渡辺敦史

    九州森林研究   73   57 - 61   2020.3

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • Effects of day length- And temperature-regulated genes on annual transcriptome dynamics in Japanese cedar (Cryptomeria japonica D. Don), a gymnosperm indeterminate species

    Mine Nose, Manabu Kurita, Miho Tamura, Michinari Matsushita, Yuichiro Hiraoka, Taiichi Iki, So Hanaoka, Kentaro Mishima, Miyoko Tsubomura, Atsushi Watanabe

    PLoS ONE   15 ( 3 )   2020.3

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    Language：Others   Publishing type：Research paper (scientific journal)  

    Seasonal phenomena in plants are primarily affected by day length and temperature. The shoot transcriptomes of trees grown in the field and a controlled-environment chamber were compared to characterize genes that control annual rhythms and the effects of day length- and temperature-regulated genes in the gymnosperm Japanese cedar (Cryptomeria japonica D. Don), which exhibits seasonally indeterminate growth. Annual transcriptome dynamics were clearly demonstrated by principal component analysis using microarray data obtained under field-grown conditions. Analysis of microarray data from trees grown in a controlled chamber identified 2,314 targets exhibiting significantly different expression patterns under short-day (SD) and long-day conditions, and 2,045 targets exhibited significantly different expression patterns at 15°C (LT; low temperature) versus 25°C. Interestingly, although growth was suppressed under both SD and LT conditions, approximately 80% of the SD- and LT-regulated targets differed, suggesting that each factor plays a unique role in the annual cycle. The top 1,000 up-regulated targets in the growth/dormant period in the field coincided with more than 50% of the SD- and LT-regulated targets, and gene co-expression network analysis of the annual transcriptome indicated a close relationship between the SD- and LT-regulated targets. These results indicate that the respective effects of day length and temperature interact to control annual transcriptome dynamics. Well-known upstream genes of signaling pathways responsive to environmental conditions, such as the core clock (LHY/CjLHYb and CCA1/CjLHYa) and PEBP family (MFT) genes, exhibited unique expression patterns in Japanese cedar compared with previous reports in other species, suggesting that these genes control differences in seasonal regulation mechanisms between species. The results of this study provide new insights into seasonal regulation of transcription in Japanese cedar.

    DOI： 10.1371/journal.pone.0229843

  • スギ精英樹のさし木苗における根系形質の遺伝的変異および地上部初期成長との関連性 Reviewed

    福田 有樹, 平岡 裕一郎, 大平 峰子, 高橋 誠, 井城 泰一, 三浦 真弘, 栗田 学, 渡辺 敦史

    日本森林学会誌   100 ( 6 )   218 - 223   2018.12

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    DOI： https://doi.org/10.4005/jjfs.100.218

  • Transcriptome dynamics of rooting zone and aboveground parts of cuttings during adventitious root formation in Cryptomeria japonica D. Don

    Yuki Fukuda, Tomonori Hirao, Kentaro Mishima, Mineko Ohira, Yuichiro Hiraoka, Makoto Takahashi, Atsushi Watanabe

    BMC Plant Biology   18 ( 1 )   2018.9

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    Language：Others   Publishing type：Research paper (scientific journal)  

    Background: Adventitious root formation is an essential physiological process for successful propagation of cuttings in various plant species. Because coniferous species are highly heterozygous, propagation of cuttings is of great practical use in breeding. Although various factors influence adventitious root formation, little is known of the associated regulatory mechanisms. Whereas adventitious roots generally form from the base of cuttings, this process is accompanied by physiological changes in leaves, which supply assimilates and metabolites. Herein, we present microarray analyses of transcriptome dynamics during adventitious root formation in whole cuttings in the coniferous species, Cryptomeria japonica. Results: Temporal patterns of gene expression were determined in the base, the middle, and needles of cuttings at eight time points during adventitious root formation. Global gene expression at the base had diverged from that in the middle by 3-h post-insertion, and changed little in the subsequent 3-days post-insertion, and global gene expression in needles altered characteristically at 3- and 6-weeks post-insertion. In Gene Ontology enrichment analysis of major gene clusters based on hierarchical clustering, the expression profiles of genes related to carbohydrates, plant hormones, and other categories indicated multiple biological changes that were involved in adventitious root formation. Conclusions: The present comprehensive transcriptome analyses indicate major transcriptional turning and contribute to the understanding of the biological processes and molecular factors that influence adventitious root formation in C. japonica.

    DOI： 10.1186/s12870-018-1401-7

  • 用土の理学的性質がスギのさし木発根性に及ぼす影響 Reviewed

    大平峰子, 花岡創, 平岡裕一郎, 栗田学, 井城泰一, 三浦真弘, 渡辺敦史

    日本森林学会誌   98 ( 6 )   265 - 272   2017.2

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • 林木育種における地上LiDAR計測の応用 : スギ精英樹F₁家系における樹幹形質の評価 Reviewed

    平岡 裕一郎, 高橋 誠, 渡辺敦史

    97 ( 6 )   290 - 295   2015.12

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • Analyses of random BAC clone sequences of Japanese cedar, Cryptomeria japonica Reviewed International journal

    田村 美帆, Hisataka, Yosuke, Moritsuka, Etsuko, 渡辺敦史, Uchiyama, Kentaro, Futamura, Norihiro, Shinohara, Kenji, Tsumura, Yoshihiko, 舘田 英典

    TREE GENETICS & GENOMES   11 ( 3 )   2015.6

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s11295-015-0859-9

  • Transcriptome sequencing and profiling of expressed genes in cambial zone and differentiating xylem of Japanese cedar (Cryptomeria japonica) Reviewed International journal

    K. Mishima, T. Fujiwara, T. Iki, K. Kuroda, K. Ymashita, M. Tamura, Y. Fujisawa, A. Watanabe

    BMC Genomics   15   219   2014.3

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    Language：English   Publishing type：Research paper (scientific journal)  

  • 関東育種基本区におけるスギ精英樹クローン雄花着花量の評価 Reviewed

    坪村美代子, 武津英太郎, 渡辺敦史

    日本森林学会誌   95 ( 3 )   156 - 162   2013.6

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

  • Genetic diversity of Pinus densiflora pollen flowing over fragmented populations during a mating season Reviewed International journal

    H. Ozawa, Watanabe Atsushi

    Journal of Forest Research   17 ( 6 )   488 - 498   2012.12

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Through field work and research, I have been able to feel the changes of the seasons and reflect the questions I have in my research

    Watanabe Atsushi

    Tree and Forest Health   28 ( 2 )   115 - 117   2024.4   （ ISSN:13440268 eISSN:21897204 ）

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    DOI： 10.18938/treeforesthealth.28.2_115

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  • Effect of the western conifer seed bug Leptoglossus occidentalis , an invasive alien insect, on seed production reduction in Japanese black pine Pinus thunbergii Reviewed

    Koji Matsunaga, Ryotaro Hara, Eitaro Fukatsu, Atsushi Watanabe, Atsushi Kume

    Journal of Forest Research   29 ( 3 )   233 - 237   2023.12   （ ISSN:1341-6979 eISSN:1610-7403 ）

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    To determine the potential reduction of the seed productivity of Japanese black pine (Pinus thunbergii) by the western conifer seed bug (Leptoglossus occidentalis), two feeding experiments were conducted in the center of Kyushu Island, southwestern Japan, in fall of 2020. First, L. occidentalis individuals and a preharvested P. thunbergii cone were enclosed in a nonwoven fabric bag for 2 weeks, which yielded a mean filled seed rate of 18.8% compared with that of 35.6% in the control. Second, L. occidentalis individuals and harvested cones were enclosed in a plastic case for 1 week, affording a mean filled seed rate of 25.7% compared with that of 36.4% in the control. These results suggest that L. occidentalis feeding reduces the filled seed rate of P. thunbergii and damages the seed orchards of this species. Additionally, P. thunbergii seeds from cones damaged by L. occidentalis could be distinguished by their germination ability when sorted using an ethanol floatation technique. Overall, these findings suggest the importance of carefully monitoring L. occidentalis infestation in P. thunbergii seed orchards.

    DOI： 10.1080/13416979.2023.2291343

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  • Simple Identifying Highly Productive Lacquer Tree (<i>Toxicodendron vernicifluum</i>) Clones by Measuring Resin Exudate Length, Tree Growth Traits, and Leaf Characteristics Reviewed

    Tabata Masanobu, Iki Taiichi, Tamura Miho, Watanabe Atsushi

    Journal of the Japanese Forest Society   105 ( 3 )   87 - 95   2023.3   （ ISSN:13498509 eISSN:1882398X ）

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    Simple Identifying Highly Productive Lacquer Tree (Toxicodendron vernicifluum) Clones by Measuring Resin Exudate Length, Tree Growth Traits, and Leaf Characteristics
    Increased production and a stable domestic supply of tree lacquer from Toxicodendron vernicifluum is essential for restoring national treasures and important cultural properties in Japan. Therefore, it is beneficial to identify clones that produce large quantities of lacquer. In this study, we used DNA markers to investigate the clonal structures of lacquer trees originating from roots at seven sites in Ibaraki Prefecture. In addition, we measured the amount of lacquer produced by several tree within each clone group. We determined the relationships between resin exudate length, tree growth traits, and leaf characteristics to find indices that can be used to indirectly estimate the amounts of lacquer produced. In total, 10 clones (A-J) were detected at the sites, with about 50％ of individuals belonging to clone E. We found that individual trees with clone groups tended to share characteristics that differed among clones. In particular, the amounts of lacquer production and diameter at breast height (DBH) were significantly different between clones. Clones with larger DBH values produced larger amounts of lacquer. Even at different sites, the growth and leaf characteristics of individuals within each clone group were very similar. Our study suggested that measurement of exuded resin, DBH, and leaf characteristics in individuals around 10-20 years old can be used to identify clones that produce large amounts of lacquer.

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  • Utility of chloroplast DNA haplotype data for ecological restoration using Fagus crenata seedlings in case of incomplete seed source information availability

    Makoto Takahashi, Susumu Goto, Yoko Fukuda, Atsushi Watanabe

    Ecological Research   38 ( 2 )   255 - 264   2023.3   （ ISSN:0912-3814 eISSN:1440-1703 ）

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    Forest trees species are often genetically adapted to local environmental conditions. Therefore, local seeds are recommended for ecological restoration. However, seedlings of broad-leaved tree species, such as Japanese beech (Fagus crenata), are limited in their commercial seedling production in Japan. Thus, long-distance transfer of seeds and/or seedlings is common. F. crenata's distinct geographical structure is well known; large-scale seed transfer may increase the risk of genetic disturbance. Several provenance trials of the species revealed that phenotypic traits such as leaf area and bud flush date differed latitudinally and/or between the Pacific Ocean side and the Japan Sea side. We investigated leaf size and bud flush date and identified the chloroplast DNA haplotype of trees planted in two provenance trials established in Hokkaido, Japan. We then examined whether cpDNA haplotype information is useful as a proxy in ecological restoration when using seedlings with incomplete seed source information. This study indicated that suitable seedlings could be selected based on chloroplast DNA haplotype information in F. crenata in the case of incomplete seed source information.

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  • Genetic diversity and structure of seed pools in an old planted Pinus thunbergii population and seed collection strategy for gene preservation

    Masakazu G. Iwaizumi, Aziz Akbar Mukasyaf, Ichiro Tamaki, Jin’ya Nasu, Naoko Miyamoto, Miho Tamura, Atsushi Watanabe

    Tree Genetics and Genomes   19 ( 1 )   2023.2   （ ISSN:1614-2942 eISSN:1614-2950 ）

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    To achieve gene preservation in tree populations when planting seedlings to regenerate forests, specific and practical guidelines and criteria for seed collection are needed to ensure reliable coverage and effective capture of the current genetic variation. We examined the genetic variation of adult trees (524 trees) and seed pools (1618 seeds) collected from 70 mother trees in an old planted population of Pinus thunbergii in Japan using seven nuclear microsatellite markers. To consider a suitable seed collection strategy, we monitored changes in allelic diversity of seed pools with an increasing number of mother trees and examined the required number of mother trees. We found significantly higher allelic diversity statistics in seed pools of mother trees with smaller diameter at breast height. Spatial genetic autocorrelation analysis showed significantly positive kinship coefficients between both pairs of adult trees and seeds collected from mother trees for up to 100-m distance classes. Based on the rarefaction curve, seed pools obtained from approximately up to 30 mother trees could cover and saturate most of the genetic diversity and composition of the adult tree population and the potential overall seed pools, particularly for statistics that are less likely affected by rare alleles. Indications from the mother tree number, together with considerations for the size or spatial distribution of mother trees, are expected to contribute not only to guidelines for in situ genetic management of local planted populations, but also to the development of strategies for ex situ gene preservation of populations as genetic resources.

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  • Evaluation of thermoregulation of different pine organs in early spring and estimation of heat reward for the western conifer seed bug (Leptoglossus occidentalis) on male cones

    Ryotaro Kitajima, Osamu Matsuda, Koji Mastunaga, Ryotaro Hara, Atsushi Watanabe, Atsushi Kume

    PLoS ONE   17 ( 8 August )   e0272565   2022.8   （ ISSN:1932-6203 eISSN:1932-6203 ）

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    The western conifer seed bug (WCSB, Leptoglossus occidentalis) is a pest of many pine species and is invasive worldwide. WCSB directly and indirectly deteriorates pine nut production by sucking seeds from cones. Currently, researchers think that WCSBs search for food by a combination of cues from visible light, infrared radiation, and chemicals such as monoterpenes. Some research revealed that WCSBs prefer larger cones, and it was thought that WCSBs suck seeds from and obtain more heat on larger cones. However, in early spring, we observed that most WCSBs gathered on male cones rather than on female cones and young cones. We hypothesized that male pine cones were warmer than female cones and needles, and WCSBs sucking male cones may receive more heat. To test these hypotheses, we measured spectral reflectance with a hyperspectral sensor and temperature of pine organs with tiny thermocouples, and the data were analyzed by a heat budget model. Our results revealed that male cones were significantly warmer and more reflective than female cones and needles, which may attract WCSBs. These results supported our hypothesis that WCSBs on male cones were warmer than those on other organs. This study will help further understanding of WCSBs and the adaptive value of pine cone colors.

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  • Detection of Plus Trees Heterozygous for a Male-sterility Gene in Hinoki Cypress (<i>Chamaecyparis obtusa</i> (Sieb. et Zucc.) Endl.), Using Self-pollinated Families Reviewed

    Miyuki Yurika, Endo Ryota, Tamura Miho, Moriguchi Yoshinari, Watanabe Atsushi

    Journal of the Japanese Forest Society   104 ( 3 )   176 - 181   2022.6   （ ISSN:13498509 eISSN:1882398X ）

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    Detection of Plus Trees Heterozygous for a Male-sterility Gene in Hinoki Cypress (Chamaecyparis obtusa (Sieb. et Zucc.) Endl.), Using Self-pollinated Families

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  • マツノザイセンチュウゲノムの多型性評価に向けたマイクロサテライト領域の探索 Reviewed

    #久島涼弥, 田村美帆, @松永孝治. 渡辺敦史

    九州森林研究   75   53 - 58   2022.3

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  • Development and characterization of EST-SSR markers for Pinus thunbergii Invited Reviewed International journal

    #Aziz Akbar Mukasyaf, Miho Tamura, Rimi Yamaguchi, Kosuke Teshima, Atsushi Watanabe

    Journal of Forest Research   1 - 4   2021.8

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    DOI： 10.1080/13416979.2021.1964152

  • Geographical cline and inter-seaside difference in cone characteristics related to climatic conditions of old planted Pinus thunbergii populations throughout Japan Invited Reviewed International journal

    @Iwaizumi, Masakazu G.; @Matsunaga, Koji; @Iki, Taiichi; @Yamanobe, Taro; @Hirao, Tomonori; Watanabe, Atsushi

    PLANT SPECIES BIOLOGY   36 ( 2 )   218 - 229   2021.4

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    DOI： 10.1111/1442-1984.12309

  • Do seedlings derived from pinewood nematode-resistant pinus thunbergii parl. clones selected in southwestern region perform well in northern regions in Japan? Inferences from nursery inoculation tests Invited Reviewed International journal

    @Matsunaga, K., @Iki, @T., Hirao, T., @Ohira, M., @Yamanobe, T., Iwaizumi, M. G., Miura, M., Isoda, K., Kurita, M., Takahashi, M. & Watanabe, A.

    Forests   11 ( 9 )   2020.9

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  • Effects of temperature factors on resistance against pine wood nematodes in pinus thunbergii, based on multiple location sites nematode inoculation tests

    Taiichi Iki, Koji Matsunaga, Tomonori Hirao, Mineko Ohira, Taro Yamanobe, Masakazu G. Iwaizumi, Masahiro Miura, Keiya Isoda, Manabu Kurita, Makoto Takahashi, Atsushi Watanabe

    Forests   11 ( 9 )   2020.9

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    Pine wilt disease (PWD) caused by the pinewood nematode (PWN) (Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle) is a worldwide issue. Infection is considered to be promoted mainly by the increased air temperature, but it is important to investigate whether the effect of high temperature similarly influences the different ranks of resistant clone. In the present study, we conducted PWN inoculation tests using six common open-pollinated families of resistant Pinus thunbergii Parl. The tests were conducted at nurseries of five test sites across Japanese archipelago between 2015 and 2017. Our analysis focused specifically on temperature. Firstly, we examined the effects of test sites, inoculation year, and their interaction on unaffected seedling rate and found that the unaffected seedling rate of all tested pine families decreased as the cumulative temperature increased. We found that the unaffected seedling rate decreased as the cumulative temperature increased for all tested pine families. In general, higher cumulative temperatures were required for having an effect on the unaffected seedling rates of higher PWN-resistant families. Typically, early cumulative temperatures, i.e., 19 days after inoculation, had the greatest effect on the unaffected seedling rates of PWN-resistant pines. However, the relationship between cumulative temperature and predicted unaffected seedling rate follow similar rate for all families. Thus, the order of resistance level is maintained in terms of the cumulative temperature required for having an effect.

    DOI： 10.3390/F11090922

  • 冬期の高温がスギの成長と材形成に与える影響の評価 Invited Reviewed

    酒本大・玉城雅範・栗田学・阪上宏樹・田村美帆・渡辺敦史

    九州森林研究   73   115 - 119   2020.3

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  • 弱病原力候補アイソレイト貝津２の評価 Reviewed

    伊藤貫太・松永孝治・山口莉未・渡辺敦史

    九州森林研究   103 - 106   2020.3

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  • Influence of temperature on pine wilt disease progression in Pinus thunbergii seedlings Reviewed

    R. Yamaguchi, K. Matsunaga, A. Watanabe

    European Journal of Plant Pathology   156 ( 2 )   581 - 590   2020.2

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    Pine wilt disease (PWD) affects forests globally and is caused by the pinewood nematode (PWN) Bursaphelenchus xylophilus. It has been proposed that PWD development has a strong association with air temperature. Because symptom development is related to the number of PWNs and they are poikilothermic animals whose development and multiplication are affected by temperature; it appears that temperature conditions affect the PWN multiplication rate, which leads to difference in disease progression. However, limited information is available about the temperatures inside pine trees, and the understanding of how temperature affects PWN multiplication and PWD progression remains incomplete. Therefore, in the present study, we evaluated the temperature, number of PWNs, and external symptoms of PWD in Pinus thunbergii seedlings infected with PWNs at air temperatures of 20 °C, 25 °C, and 30 °C over time. We found that the seedling temperatures were slightly higher than the air temperature under each temperature condition and that the effective accumulated temperature calculated using a base temperature of 10 °C was related to the number of PWNs and symptom development in all temperature test groups. However, at 20 °C, there were very few PWNs in some seedlings and none of the seedlings showed partial or full wilting. These findings suggest that PWD progression is greatly affected by ambient temperature and facilitated by increase in PWN populations with effective accumulated temperature especially in the range of 25 °C–30 °C.

    DOI： 10.1007/s10658-019-01908-7

  • DNAマーカーを利用した日本に現存するウルシ林の遺伝的多様性評価 Invited Reviewed

    渡辺 敦史, 田村 美帆, 泉 湧一郎, 山口 莉未, 井城 泰一, 田端 雅進

    101 ( 6 )   298 - 304   2019.12

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    DOI： https://doi.org/10.4005/jjfs.101.298

  • Construction of genetic linkage map and identification of a novel major locus for resistance to pine wood nematode in Japanese black pine (Pinus thunbergii) Reviewed

    Tomonori Hirao, Koji Matsunaga, Hideki Hirakawa, Kenta Shirasawa, Keiya Isoda, Kentaro Mishima, Miho Tamura, Atsushi Watanabe

    BMC plant biology   19 ( 1 )   2019.10

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    Background: Pine wilt disease (PWD), which is caused by the pine wood nematode (PWN) Bursaphelenchus xylophilus, is currently the greatest threat to pine forests in Europe and East Asian countries including Japan. Constructing a detailed linkage map of DNA markers and identifying PWD resistance genes/loci lead to improved resistance in Pinus thunbergii, as well as other Pinus species that are also susceptible to PWD. Results: A total F1 mapping population of 188 individuals derived from a cross between the PWD-resistant P. thunbergii varieties 'Tanabe 54' (resistant rank 2 to PWD) and 'Tosashimizu 63' (resistant rank 4 to PWD) was inoculated with PWN, and was evaluated for disease symptoms. To perform linkage analysis for PWN resistance, a set of three maps was constructed; two parental maps generated using the integrated two-way pseudo-testcross method, and a consensus map with population-type cross-pollination. The linkage map of 'Tanabe 54' consisted of 167 loci, and covered 14 linkage groups (LGs), with a total genetic distance of 1214.6 cM. The linkage map of 'Tosashimizu 63' consisted of 252 loci, and covered 14 LGs, with a total genetic distance of 1422.1 cM. The integrated consensus map comprised 12 LGs with the basic chromosome number of P. thunbergii, and a total genetic distance of 1403.6 cM. Results from quantitative trait loci (QTL) analysis using phenotype data and linkage maps indicated that PWN resistance is controlled by a single dominant allele, which was derived from the 'Tanabe 54' female parent. This major QTL was located on linkage group 3 and was designated PWD1 for PINE WILT DISEASE 1. Conclusions: The PWD1 locus is a major resistance QTL located on the Pinus consensus LG03 that acts in a dominant manner to confer pine wood nematode resistance. Information from the present study will be useful for P. thunbergii breeding programs to improve resistance to PWD, and also to help identify susceptibility genes in Pinus species.

    DOI： 10.1186/s12870-019-2045-y

  • リュウキュウマツ樹体内でのマツノザイセンチュウの増殖とPR遺伝子の応答 Reviewed

    山口莉未・桑江喬也・伊藤貫太・玉城雅範・渡辺敦史

    九州森林研究   ( 72 )   95 - 98   2019.3

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  • The gene expression analysis of Arabidopsis thaliana ABC transporters by real-time PCR for screening monolignol-transporter candidates Reviewed

    Manami Takeuchi, Atsushi Watanabe, Miho Tamura, Yuji Tsutsumi

    Journal of Wood Science   64 ( 5 )   477 - 484   2018.10

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    The transport of monolignols from the cytosol to the cell wall is essential for lignin synthesis. The ATP-binding cassette (ABC) transporters may be involved in the transport of lignin precursors. ABC transporter genes subjected to expression analysis were chosen based on two criteria for screening candidate transporter genes related to lignification. The expression levels of 15 target genes in five plant organs were analyzed by real-time PCR. Five transporter genes (ABCG29, ABCG30, ABCG33, ABCG34, and ABCG37), which were simultaneously expressed with the reference genes, were selected as candidates. The candidate gene expression levels in root tissues of T-DNA insertion mutants were determined by semi-quantitative reverse transcription PCR. ABCG30 was more highly expressed in the abcg34 mutant than in the wild-type plants, while the expression of ABCG34 was twofold higher in the abcg30 mutant plants than in the wild-type plants. Thus, the expression of ABCG30 and ABCG34 may affect each other. There was no significant change in lignin content and composition in the single-gene knockout mutants of the candidate transporter genes, which suggested that each candidate gene did not solely contribute to lignin synthesis.

    DOI： 10.1007/s10086-018-1733-9

  • Potential of genome-wide studies in unrelated plus trees of a coniferous species, cryptomeria japonica (japanese cedar) Reviewed

    Yuichiro Hiraoka, Eitaro Fukatsu, Kentaro Mishima, Tomonori Hirao, Kosuke Teshima, Miho Tamura, Miyoko Tsubomura, Taiichi Iki, Manabu Kurita, Makoto Takahashi, Atsushi Watanabe

    Frontiers in Plant Science   9   2018.9

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    A genome-wide association study (GWAS) was conducted on more than 30,000 single nucleotide polymorphisms (SNPs) in unrelated first-generation plus tree genotypes from three populations of Japanese cedar Cryptomeria japonica D. Don with genomic prediction for traits of growth, wood properties and male fecundity. Among the assessed populations, genetic characteristics including the extent of linkage disequilibrium (LD) and genetic structure differed and these differences are considered to be due to differences in genetic background. Through population-independent GWAS, several significant SNPs found close to the regions associated with each of these traits and shared in common across the populations were identified. The accuracies of genomic predictions were dependent on the traits and populations and reflected the genetic architecture of traits and genetic characteristics. Prediction accuracies using SNPs selected based on GWAS results were similar to those using all SNPs for several combinations of traits and populations. We discussed the application of genome-wide studies for C. japonica improvement.

    DOI： 10.3389/fpls.2018.01322

  • Expression analysis of transporter genes for screening candidate monolignol transporters using Arabidopsis thaliana cell suspensions during tracheary element differentiation Reviewed

    Manami Takeuchi, Takahiro Kegasa, Atsushi Watanabe, Miho Tamura, Yuji Tsutsumi

    Journal of Plant Research   131 ( 2 )   297 - 305   2018.3

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    The mechanism of monolignol transportation from the cytosol to the apoplast is still unclear despite being an essential step of lignification. Recently, ATP-binding cassette (ABC) transporters were suggested to be involved in monolignol transport. However, there are no reliable clues to the transporters of the major lignin monomers coniferyl and synapyl alcohol. In this study, the lignification progress of Arabidopsis cultured cells during tracheary element differentiation was monitored. The expression of selected transporter genes, as well as lignification and cell-wall formation related genes as references, in differentiating cultured cell samples harvested at 2-day intervals was analyzed by real-time PCR and the data were statistically processed. The cell wall formation transcription factor MYB46, programmed-cell death related gene XCP1 and lignin polymerization peroxidase AtPrx25 were classified into the same cluster. Furthermore, the cluster closest to the abovementioned cluster contained the lignin synthesis transcription factor MYB58 and the Arabidopsis ABC transporters ABCG11, ABCG22, ABCG36 and ABCG29. This result suggested that these four ABC transporters may be involved in lignification. In the expression analysis, unexpectedly, the lignification-related genes CAD5 and C4H were not included in the same cluster as MYB58 and AtPrx25. The expression data also suggested that the lignification of tracheary elements in the culture, where lignification ratio finally reached to around 40%, continued after cell death because lignification actively progressed after programmed cell death-related gene started to be expressed.

    DOI： 10.1007/s10265-017-0979-4

  • 異なる接種密度で接種した際のクロマツ樹体内におけるマツノザイセンチュウの頭数評価 Reviewed

    山口莉未,松永孝治,渡辺敦史

    九州森林研究   ( ７１ )   63 - 66   2018.3

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  • The origin of wild populations of Toxicodendron succedaneum on mainland Japan revealed by genetic variation in chloroplast and nuclear DNA Reviewed

    Yuichiro Hiraoka, Ichiro Tamaki, Atsushi Watanabe

    Journal of Plant Research   131 ( 2 )   225 - 238   2018.3

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    Toxicodendron succedaneum: (L.) Kuntze is a tree cultivated for the production of sumac wax, which is extracted from the mesocarp. There are several hypotheses regarding the origin of T. succedaneum on mainland Japan. In this study, the geographical distribution of genetic variation in 13 wild populations on Honshu, Shikoku, Kyushu, and Ryukyu Islands, Japan was investigated and compared with that of individuals from continental Asia. Seven chloroplast DNA haplotypes of T. succedaneum were observed in Japan and could be divided into three lineages based on relatedness between haplotypes. One of these lineages was also observed in continental Asia, and the others were genetically distant from the haplotypes that originated on the continent, with one considered to have originated on the Ryukyu Islands, and the other on mainland Japan. The genetic diversity of both chloroplast and nuclear DNA was lower in populations from Ryukyu Islands than in populations from mainland Japan. Bayesian clustering based on nuclear genotypes showed a clear difference between the groups from Ryukyu Islands and mainland Japan. Based on approximate Bayesian computation analysis of polymorphic data for both genomes, it was inferred that wild populations of T. succedaneum on mainland Japan consist of both lineages with natural distribution on mainland Japan and those introduced from Ryukyu Islands and continental Asia.

    DOI： 10.1007/s10265-017-0992-7

  • Species characteristics and intraspecific variation in growth and photosynthesis of Cryptomeria japonica under elevated O 3 and CO 2 Reviewed

    Yuichiro Hiraoka, Taiichi Iki, Mine Nose, Hiroyuki Tobita, Kenichi Yazaki, Atsushi Watanabe, Yoshitake Fujisawa, Mitsutoshi Kitao

    Tree Physiology   37 ( 6 )   733 - 743   2017.6

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    In order to predict the effects of future atmospheric conditions on forest productivity, it is necessary to clarify the physiological responses of major forest tree species to high concentrations of ozone (O 3) and carbon dioxide (CO 2). Furthermore, intraspecific variation of these responses should also be examined in order to predict productivity gains through tree improvements in the future. We investigated intraspecific variation in growth and photosynthesis of Cryptomeria japonica D. Don, a major silviculture species in Japan, in response to elevated concentrations of O 3 (eO 3) and CO 2 (eCO 2), separately and in combination. Cuttings of C. japonica were grown and exposed to two levels of O 3 (ambient and twice-ambient levels) in combination with two levels of CO 2 (ambient and 550 μmol mol '1 in the daytime) for two growing seasons in a free-air CO 2 enrichment experiment. There was no obvious negative effect of eO 3 on growth or photosynthetic traits of the C. japonica clones, but a positive effect was observed for annual height increments in the first growing season. Dry mass production and the photosynthetic rate increased under eCO 2 conditions, while the maximum carboxylation rate decreased. Significant interaction effects of eO 3 and eCO 2 on growth and photosynthetic traits were not observed. Clonal effects on growth and photosynthetic traits were significant, but the interactions between clones and O 3 and/or CO 2 treatments were not. Spearman's rank correlation coefficients between growth traits under ambient conditions and for each treatment were significantly positive, implying that clonal ranking in growth abilities might not be affected by either eO 3 or eCO 2. The knowledge obtained from this study will be helpful for species selection in afforestation programs, to continue and to improve current programs involving this species, and to accurately predict the CO 2 fixation capacity of Japanese forests.

    DOI： 10.1093/treephys/tpx028

  • Erratum to: Transcriptome sequencing and profiling of expressed genes in cambial zone and differentiating xylem of Japanese cedar (Cryptomeria japonica)[BMC Genomics, 15, (2014) (219)], DOI: 10.1186/1471-2164-15-219

    Kentaro Mishima, Takeshi Fujiwara, Taiichi Iki, Katsushi Kuroda, Kana Yamashita, Miho Tamura, Yoshitake Fujisawa, Atsushi Watanabe

    BMC Genomics   17 ( 1 )   2016.10

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    In the original publication of this article [1], the accession number was listed as DC882454 through DC883482 on page 27; instead, the number should be DRA000525.

    DOI： 10.1186/s12864-016-3156-6

  • Biological Activities of Extracts from Different Parts of Cryptomeria japonica Invited Reviewed International journal

    Horiba, Hiroki, Nakagawa, Toshinori, Zhu, Qinchang, Ashour, Ahmed, 渡辺敦史, K.Shimizu

    NATURAL PRODUCT COMMUNICATIONS   11 ( 9 )   1337 - 1342   2016.9

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  • スギ在来品種の成長パターンおよび選抜への遺伝と植栽密度の影響 Reviewed

    佐藤 嘉彦, 武津 英太郎, 平岡 裕一郎, 渡辺 敦史, 高橋 誠

    日本森林学会誌   98 ( 2 )   45 - 52   2016.6

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    We analyzed the effect of genotype and planting density on the growth patterns of height and diameter at breast height (DBH) and the efficiency of indirect early selection targeting on the low planting density using stem analysis data obtained from a test site composed of 6 cultivars and 3 planting densities (5, 000, 3, 000, 1, 500 trees/ha) at stand age 28. The growth pattern of height was mainly affected by genotype, but the effect of planting density was small. The growth pattern of DBH was affected by both genotype and planting density. Our results showed the importance of selection of the genotype of seedlings for the control of growth in plantations of Sugi. The results of genetic evaluation obtained at general planting density (3, 000 trees/ha) could be applied for the prediction of genetic performance of growth traits at different planting densities. Approximately 15 years would be required to obtain 80% of genetic gain at stand age 28 with the plantation of 1, 500 trees/ha according to the test results obtained at the plantation of general planting density (3, 000 trees/ha).

    DOI： https://doi.org/10.4005/jjfs.98.45

  • スギ在来品種の成長パターンおよび選抜への遺伝と植栽密度の影響 Reviewed

    佐藤嘉彦, 武津英太郎, 平岡裕一郎, 渡辺敦史, 高橋誠

    日本森林学会誌   98 ( 2 )   45 - 52   2016.4

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    The effect of genotype and planting density on the growth patterns and selection of local varieties of sugi (Cryptomeria japonica).
    We analyzed the effect of genotype and planting density on the growth patterns of height and diameter at breast height (DBH) and the efficiency of indirect early selection targeting on the low planting density using stem analysis data obtained from a test site composed of 6 cultivars and 3 planting densities (5,000, 3,000, 1,500 trees/ha) at stand age 28. The growth pattern of height was mainly affected by genotype, but the effect of planting density was small. The growth pattern of DBH was affected by both genotype and planting density. Our results showed the importance of selection of the genotype of seedlings for the control of growth in plantations of Sugi. The results of genetic evaluation obtained at general planting density (3,000 trees/ha) could be applied for the prediction of genetic performance of growth traits at different planting densities. Approximately 15 years would be required to obtain 80％ of genetic gain at stand age 28 with the plantation of 1,500 trees/ha according to the test results obtained at the plantation of general planting density (3,000 trees/ha).

    DOI： 10.4005/jjfs.98.45

  • Highly polymorphic nuclear microsatellite markers reveal detailed patterns of genetic variation in natural populations of Yezo spruce in Hokkaido Reviewed International journal

    Iwazumi, Masakazu G, Aizawa, Mineaki, 渡辺敦史, Goto, Susumu

    JOURNAL OF FOREST RESEARCH   20 ( 2 )   2015.4

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    DOI： 10.1007/s10310-014-0477-7

  • Current genetic structure of teak (Tectona qrandis) in Myanmar based on newly developed chloroplast single nucleotide polymorphism and nuclear single sequence repeat markers Reviewed International journal

    Thwe-Thwe-Win, Hirao, Tomonori, 渡辺敦史, Goto, Susumu

    TROPICAL CONSERVATION SCIENCE   8 ( 1 )   2015.3

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  • Population genetic structure and the effect of historical human activity on the genetic variability of Cryptomeria japonica core collection, in Japan Reviewed International journal

    Uchiyama, Kentaro, Miyamoto, Naoko, Takahashi, Makoto, 渡辺敦史, Tsumura, Yoshihiko

    TREE GENETICS & GENOMES   10 ( 5 )   2014.10

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    DOI： 10.1007/s11295-014-0758-5

  • Genetic structures of Calophyllum inophyllum L., a tree employing sea-drift seed dispersal in the northern extreme of its distribution

    So Hanaoka, Ching Te Chien, Shun Ying Chen, Atsushi Watanabe, Suzuki Setsuko, Kazutaka Kato

    Annals of Forest Science   71 ( 5 )   575 - 584   2014.7

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    • Context: Little is known about the potential of gene flow and resulting genetic structures of trees employing sea-drifting seed dispersal in island populations. • Aims: Current genetic structure and the magnitude of historical gene flow were estimated in island populations of Calophyllum inophyllum L., a typical plant employing sea-drifting seed dispersal. • Methods: Samples were collected from the northern extreme of the species' distribution (Taiwan and the Sakishima, Daito, and Ogasawara Islands, Japan) and genotyped using 15 EST-SSR markers. Genetic differentiation (F ST and AMOVA), genetic structure (STRUCTRE analysis), and historical gene flow (assignment testing) were determined. • Results: Frequent gene flow within and rare gene flow among island groups was determined using assignment testing. Clear genetic structures were also detected using the STRUCTURE analysis, which demonstrated differentiation between dominant clusters among geographically constructed island groups. • Conclusions: The potential for gene flow via sea-drifting seed dispersal was high, and this was possible even among small islands. However, the extent and frequency of gene flow were not great enough to prevent genetic differentiation in a range of over a few hundred kilometers. © 2014 INRA and Springer-Verlag France.

    DOI： 10.1007/s13595-014-0365-5

  • Comparison of histological responses and tissue damage expansion between resistant and susceptible Pinus thunbergii infected with pine wood nematode Bursaphelenchus xylophilus. Reviewed International journal

    D. kusumoto, T. Yonemichi, H. Inoue, T. Hirao, A. Watanabe, T. Yamada

    Journal of Forest Research   19   285 - 294   2014.4

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  • Influence of long-distance seed dispersal on the genetic diversity of seed rain in fragmented Pinus densiflora populations relative to Pollen-Mediated gene flow

    Hajime Ozawa, Atsushi Watanabe, Kentaro Uchiyama, Yoko Saito, Yuji Ide

    Journal of Heredity   104 ( 4 )   465 - 475   2013.7

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    Long-distance dispersal (LDD) of seeds has a critical impact on species survival in patchy landscapes. However, relative to pollen dispersal, empirical data on how seed LDD affects genetic diversity in fragmented populations have been poorly reported. Thus, we attempted to indirectly evaluate the influence of seed LDD by estimating maternal and paternal inbreeding in the seed rain of fragmented 8 Pinus densiflora populations. In total, the sample size was 458 seeds and 306 adult trees. Inbreeding was estimated by common parentage analysis to evaluate gene flow within populations and by sibship reconstruction analysis to estimate gene flow within and among populations. In the parentage analysis, the observed probability that sampled seeds had the same parents within populations was significantly larger than the expected probability in many populations. This result suggested that gene dispersal was limited to within populations. In the sibship reconstruction, many donors both within and among populations appeared to contribute to sampled seeds. Significant differences in sibling ratios were not detected between paternity and maternity. These results suggested that seed-mediated gene flow and pollen-mediated gene flow from outside population contributed some extent to high genetic diversity of the seed rain (HE > 0.854). We emphasize that pine seeds may have excellent potential for gene exchange within and among populations. © The American Genetic Association. 2013. All rights reserved.

    DOI： 10.1093/jhered/est022

  • Development of tetranucleotide microsatellite markers in Pinus kesiya Royle ex Gordon

    D. V. Thao, M. Yamashita, A. Watanabe, S. Shiraishi

    Conservation Genetics Resources   5 ( 2 )   405 - 407   2013.6

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    Pinus kesiya Royle ex Gordon (P. kesiya) is one of the economically and ecologically important pine species in South-East Asian. 12 polymorphic tetranucleotide microsatellite markers were isolated from this species using a dual-suppression polymerase chain reaction (PCR) technique and these markers were characterized in 24 individuals collected from a natural population in the Southern Vietnam. The number of alleles per locus ranged from 2 to 28, and expected heterozygosities of the 12 loci ranged from 0. 198 to 0. 954, with an average of 0. 685. These polymorphic markers will serve as useful tools for studies of conservation genetics in P. kesiya and probably in its closely related species. © 2012 Springer Science+Business Media Dordrecht.

    DOI： 10.1007/s12686-012-9814-3

  • Isolation and characterization of tetranucleotide microsatellite markers for Pinus merkusii

    D. V. Thao, A. Y.P.B.C. Widyatmoko, L. Guan, E. Gotoh, A. Watanabe, S. Shiraishi

    Conservation Genetics Resources   5 ( 2 )   433 - 436   2013.6

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    Pinus merkusii is an important tree native to Southeast Asia. Thirteen tetranucleotide microsatellite makers were developed from this species using a dual-suppression polymerase chain reaction technique. Of these markers, nine loci were polymorphic. The mean number of alleles per locus was 3. 18 (range 2-5) and mean expected heterozygosities were 0. 525 (range 0. 384-0. 712). These microsatellite markers will be available to assess the genetic diversity, mating patterns and population structure which will facilitate conservation and management strategies. © 2012 Springer Science+Business Media Dordrecht.

    DOI： 10.1007/s12686-012-9821-4

  • The promoter of an A9 homolog from the conifer Cryptomeria japonica imparts male strobilus-dominant expression in transgenic trees Reviewed International journal

    Kurita ,M, Konagaya, K, Watanabe Atsushi, Kondo T, Ishii, K, Taniguchi, T

    Plant Cell Report   328 ( 2 )   319 - 328   2013.2

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  • Development of tetranucleotide microsatellite markers in Pinus kesiya Royle ex Gordon. Reviewed International journal

    Thao, DV, Yamashita, M, Watanabe Atsushi, Susumu Shiraishi

    Conservation Genetics Resources   5 ( 2 )   405 - 407   2013.1

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  • Isolation and characterization of tetranucleotide microsatellite markers for Pinus merkusii Reviewed International journal

    Thao, DV, AYPBC. Widyatmoko, Guan, L, Eiji Gotoh, Watanabe Atsushi, Susumu Shiraishi

    Conservation Genetics Resources   5 ( 2 )   433 - 436   2013.1

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  • Isolation and characterization of microsatellite markers for Thujopsis dolabrata var. hondai (Cupressaceae) Reviewed International journal

    Mishima, K, Hirao, T, Watanabe Atsushi, Takata, K

    the American Journal of Botany   99 ( 8 )   317 - 319   2012.8

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  • Isolation and characterization of microsatellite markers in Melia volkensii Gurke. Reviewed International journal

    Hanakoka, S, Muturi, G,M, Watanabe Atsushi

    Conservation Genetics Resource   4 ( 2 )   395 - 398   2012.6

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  • Phylogeographical structure in Zelkova serrata in Japan and phylogeny in the genus Zelkova using the polymorphisms of chloroplast DNA. Reviewed International journal

    Fukatsu, E, Watanabe Atsushi, Nakada, R, Isoda, K, Hirao, T, Ubukata, M, Koyama, Y, Kodani, J, Saito, M, Miyamoto, N, Takahashi, M

    Conservation Genetics   13   1109 - 1118   2012.5

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  • Extended linkage disequilibrium in non-coding regions in a conifer, Cryptomeria japonica Reviewed International journal

    Moritsuka, E, Hisataka, Y, Tamura, M, Watanabe Atsushi, Tsumura, Y, Hidenori Tachida

    Genetics   190 ( 3 )   1145 - 1148   2012.3

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  • Japanese beech (Fagus crenata) plantations established from seedlings of non-native genetic lineages Reviewed International journal

    Koyama, Y, Takahashi, M, Murauchi, Y, Fukatsu, E, Watanabe Atsushi, Tomaru, N

    Journal of Forest Research   17 ( 1 )   116 - 120   2012.2

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  • Characterization of resistance to pine wood nematode infection in Pinus thunbergii using suppression subtractive hybridization

    Tomonori Hirao, Eitaro Fukatsu, Atsushi Watanabe

    BMC Plant Biology   12   2012.1

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    Background: Pine wilt disease is caused by the pine wood nematode, Bursaphelenchus xylophilus, which threatens pine forests and forest ecosystems worldwide and causes serious economic losses. In the 40 years since the pathogen was identified, the physiological changes occurring as the disease progresses have been characterized using anatomical and biochemical methods, and resistant trees have been selected via breeding programs. However, no studies have assessed the molecular genetics, e.g. transcriptional changes, associated with infection-induced physiological changes in resistant or susceptible trees.Results: We constructed seven subtractive suppression hybridization (SSH) cDNA libraries using time-course sampling of trees inoculated with pine wood nematode at 1, 3, or 7 days post-inoculation (dpi) in susceptible trees and at 1, 3, 7, or 14 dpi in resistant trees. A total of 3,299 sequences was obtained from these cDNA libraries, including from 138 to 315 non-redundant sequences in susceptible SSH libraries and from 351 to 435 in resistant SSH libraries. Using Gene Ontology hierarchy, those non-redundant sequences were classified into 15 subcategories of the biological process Gene Ontology category and 17 subcategories of the molecular function category. The transcriptional components revealed by the Gene Ontology classification clearly differed between resistant and susceptible libraries. Some transcripts were discriminative: expression of antimicrobial peptide and putative pathogenesis-related genes (e.g., PR-1b, 2, 3, 4, 5, 6) was much higher in susceptible trees than in resistant trees at every time point, whereas expression of PR-9, PR-10, and cell wall-related genes (e.g., for hydroxyproline-rich glycoprotein precursor and extensin) was higher in resistant trees than in susceptible trees at 7 and 14 dpi.Conclusions: Following inoculation with pine wood nematode, there were marked differences between resistant and susceptible trees in transcript diversity and the timing and level of transcripts expressed in common; in particular, expression of stress response and defense genes differed. This study provided new insight into the differences in the physiological changes between resistant and susceptible trees that have been observed in anatomical and biochemical studies. © 2012 Hirao et al; licensee BioMed Central Ltd.

    DOI： 10.1186/1471-2229-12-13

  • 関東育種基本区におけるスギ精英樹クローンの立木材質の評価 Reviewed

    三嶋賢太郎, 井城泰一, 平岡裕一郎, 宮本尚子, 渡辺 敦史

    木材学会誌   57 ( 5 )   256 - 265   2011.9

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  • ハゼノキの在来品種, 優良候補個体およびアジア大陸と沖縄島の自生個体における葉緑体ハプロタイプの比較 Reviewed

    平岡裕一郎, 渡辺 敦史

    日本森林学会誌   93 ( 4 )   200 - 204   2011.8

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  • Spatiotemporal gene expression profiles associated with male strobilus development in Cryptomeria japonica by suppression subtractive hybridization Reviewed International journal

    Kurita, M, Taniguchi, T, Nakada, N, Kondo, T, Watanabe Atsushi

    Breeding Science   61 ( 2 )   174 - 182   2011.6

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  • Development of Highly Polymorphic Nuclear Microsatellite Markers for Hinoki (Chamaecyparis obtusa) Reviewed International journal

    Iwaizumi, MG, Watanabe Atsushi, Isoda, K

    Silvae Genetica   60 ( 2 )   62 - 65   2011.5

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  • Simulation evaluation of paternal and maternal immigrant gene flow and the implications for the overall genetic composition of Pinus densiflora dispersed seeds Reviewed International journal

    Iwaizumi, M.G, Takahashi, M, Watanabe Atsushi, Ubukata, M

    Journal of Heredity   101 ( 2 )   144 - 153   2010.11

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  • Development and Characterization of Microsatellites，Clone Identification and Determination of Genetic Relationships among Rhus succedanea L. Individuals Reviewed International journal

    Hiraoka, Y, Watanabe Atsushi

    Journal of the Japanese Society for Horticultual Science   79 ( 2 )   141 - 149   2010.4

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  • Isolation and characterization of microsatellite markers from Robinia pseudoacacia L. Reviewed International journal

    Mishima, K, Hirao, T, Watanabe Atsushi, Takata, K

    Molecular Ecology resources   9 ( 3 )   850 - 852   2009.5

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  • A frameshift mutation of the chloroplast matK coding region is associated with chlorophyll deficiency in the Cryptomeria japonica virescent mutant Wogon-Sugi Reviewed International journal

    Hirao,T, Watanabe Atsushi, Kurita, M, Kondo, T, Takata, K

    Current Genetic   55 ( 3 )   311 - 321   2009.5

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  • Development and characterization of chloroplast microsatellite markers for Cryptomeria japonica D. Don Reviewed International journal

    Hirao,T, Watanabe Atsushi, Miyamoto, N, Takata, K

    Molecular Ecology resources   9 ( 1 )   122 - 124   2009.1

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Use of different seed tissues for separate bi-parental identification of dispersed seeds in conifers: confirmation and practices for gene flow in Pinus densiflora. Reviewed International journal

    Iwaizumi, M.G, Watanabe Atsushi, Ubukata, M

    Canadian journal of Forest research   37 ( 10 )   2022 - 2030   2007.10

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  • Isolation and characterization of microsatellite loci from Larix kaempferi Reviewed International journal

    Isoda, K, Watanabe Atsushi

    Molecular Ecology note   6 ( 3 )   664 - 666   2006.9

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  • Isolation and characterization of microsatellite loci from Quercus mongolica var. crispula

    Kentaro Mishima, Atsushi Watanabe, Keiya Isoda, Masatoshi Ubukata, Katsuhiko Takata

    Molecular Ecology Notes   6 ( 3 )   695 - 697   2006.9

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    Microsatellites were isolated and characterized for the Japanese oak species, Quercus mongolica var. crispula, distributed in temperate deciduous forests of Japan. Eleven of the 48 primer pairs designed successfully amplified unambiguous and polymorphic single loci among 67 Q. mongolica var. crispula individuals within a plot in southwestern Japan. The observed and expected heterozygosities of the 11 microsatellite markers ranged from 0.522 to 0.896 and from 0.536 to 0.882, respectively. These polymorphic microsatellite markers are useful for estimating pollen-mediated gene flow in Q. mongolica var. crispula. © 2006 The Authors.

    DOI： 10.1111/j.1471-8286.2006.01313.x

  • SSRマーカーを利用したスギ精英樹の個体識別 Reviewed

    平尾知士, 渡辺 敦史, 福田陽子, 近藤禎二, 高田克彦

    日本森林学会誌   88 ( 3 )   202 - 205   2006.6

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  • Isolation of microsatellite markers from Pinus densiflora Sieb. et Zucc. using a dual PCR technique

    A. Watanabe, M. G. Iwaizumi, M. Ubukata, T. Kondo, C. Lian, T. Hogetsu

    Molecular Ecology Notes   6 ( 1 )   80 - 82   2006.3

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    We developed seven microsatellite loci from Pinus densiflora using a dual polymerase chain reaction (PCR) technique. Of 186 clones from a library based on suppression PCR, 127 contained microsatellite sequences. Of these, 43 candidates were determined sequences of both flanking regions, and 16 regions from this group were chosen as development markers. Seven of these primer pairs successfully amplified polymorphic single loci among 83 resistant trees against pine wood nematode. The observed heterozygosity of the seven microsatellite markers ranged from 0.247 to 0.843. Mendelian inheritance was confirmed using megagametophytes. © 2006 Blackwell Publishing Ltd.

    DOI： 10.1111/j.1471-8286.2005.01145.x

  • Development and characterization of simple sequence repeat DNA markers for Zelkova serrata Reviewed International journal

    Fukatsu, E, Isoda, K, Hirao, T, Tkahashi, M, Watanabe Atsushi

    Molecular Ecology note   5 ( 2 )   378 - 380   2005.6

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  • Discrimination of Teak (Tectona grandis L.) plus trees using selected random amplified polymorphic DNA (RAPD) markers Reviewed International journal

    Watanabe Atsushi, AYPBC. Widyatmoko, A. Rimbawanto, Susumu Shiraishi

    Journal of Tropical Forest Science   16 ( 1 )   17 - 24   2004.1

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  • RAPDマーカーによるスギカミキリ抵抗性個体の識別 Reviewed

    西山和美, 渡辺 敦史, 久保田正裕

    日本林学会誌   84 ( 4 )   262 - 266   2002.11

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  • RAPDマーカーを用いたハゼノキ品種の識別 Reviewed

    後藤晋, 渡辺 敦史, 池田勝正

    日本林学会誌   79 ( 4 )   229 - 233   1997.11

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  • PCR-SSCP分析を用いたアカマツとクロマツの葉緑体ゲノム型の簡便判定法 Reviewed

    渡辺 敦史, 河崎久男, 白石 進

    日本林学会誌   79 ( 3 )   155 - 156   1997.8

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  • RAPD variation among Quercus species distributed in temperate deciduous forests of the the Hiruzen Mountains Reviewed International journal

    Lee, JH, H. Hahizume, Watanabe Atsushi, T. Fukata, Susumu Shiraishi, F. Yamamoto

    Journal of Forest Research   2 ( 2 )   121 - 123   1997.5

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  • DNA分子マーカーによるアカクロマツ（Pinus×densi-thubergii）のゲノム解析　—その雑種性の検証— Reviewed

    渡辺 敦史, 白石 進, 川瀬英治, 戸田忠雄, 那須仁哉

    日本林学会誌   78 ( 3 )   293 - 300   1996.8

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  • ヒノキとサワラの種間雑種および園芸品種のDNA分析 Reviewed

    楢崎康二, 渡辺 敦史, 冨田啓治, 佐々木義則, 白石 進

    日本林学会誌   78 ( 2 )   157 - 161   1996.5

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  • 蔵王山系馬ノ神岳に生存するカラマツのDNA分類学的解析 Reviewed

    白石 進, 磯田圭哉, 渡辺 敦史, 河崎久男

    日本林学会誌   78 ( 2 )   175 - 182   1996.5

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  • rbcL遺伝子多型を利用したアカマツとクロマツの葉緑体ゲノム識別 Reviewed

    白石 進, 渡辺 敦史

    日本林学会誌   77 ( 5 )   429 - 436   1995.9

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  • Reproductive success of pollen derived from selected and Non-selected sources and its impact on the performance of crops in a Nematode-Resistant Japanese Black pine seed orchard Reviewed International journal

    Goto, S, Watanabe Atsushi, Miyahara, F, Mori, Y

    Silvae Genetica   1900

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Characterization of resistance and susceptibility to pine wood nematode infection in Pinus thunbergii using suppression subtractive hybridization approach. Reviewed

    Hirao, T, Fukatsu E, Watanabe Atsushi

    BMC Plant Biology   1900

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    Language：English   Publishing type：Research paper (scientific journal)  

  • 材線虫病抵抗性マツ類における組織の反応と線虫の分布・増殖 Reviewed

    楠本 大, 米道 学, 村田 政穂, 渡辺 敦史, 磯田 圭哉, 平尾 知士, 山田 利博

    樹木医研究   1900

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  • The impact of phenological and artificial factors on seed quality in a nematode-resistant Pinus densiflora seed orchard Reviewed International journal

    Ozawa, H, Watanabe, J, Chen, H, Isoda, K, Watanabe Atsushi

    Silvae Genetica   1900

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  • Complete nucleotide sequence of the Cryptomeria japonica D. Don. chloroplast genome and comparative chloroplast genomics: diversified genomic structure of coniferous species Reviewed International journal

    Hirao, T, Watanabe Atsushi, Kurita, M, Kondo, T, Takata, K

    BMC Plant Biology   1900

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Books

  • 各都道府県の林業・林産業と遺伝育種の関わり

    渡辺敦史, @来田和人, @久保田正裕, @後藤晋（Role：Edit）

    2022.3 

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    Language：Japanese   Book type：Scholarly book

    森林遺伝育種学会誌にこれまで掲載されてきたシリーズを編集し、単行本として出版した

  • 森林遺伝育種学

    渡辺 敦史（Role：Joint author）

    文永堂出版  2012.10 

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    Responsible for pages：第２章１　遺伝学の基本　20-39   Language：Japanese   Book type：General book, introductory book for general audience

  • 各都道府県の林業・林産業と遺伝育種の関わり

    渡辺 敦史, 来田 和人, 久保田 正裕, 後藤 晋

    森林遺伝育種学会  2022    （ ISBN:9784944005345 ）

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    Language：Japanese  

    CiNii Books

  • 樹木医学入門

    渡辺敦史（Role：Joint author）

    朝倉出版  2021.4 

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    Language：Japanese   Book type：Scholarly book

  • 植物特性と最新植栽技術

    船田, 良, 渡辺, 敦史

    農山漁村文化協会  2020.3 

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    Responsible for pages：総ページ数：viii, 125p   Language：Japanese  

  • 地域資源を活かす　生活工芸双書　漆　２　植物特性と最新植栽技術

    渡辺敦史（Role：Joint author）

    2020.3 

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    Language：Japanese   Book type：General book, introductory book for general audience

  • 森の分子生態学２

    平尾知士, 渡辺 敦史（Role：Joint author）

    文一総合出版  2012.3 

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    Responsible for pages：第３部解析手法編第２章オルガネラDNA解析手法，193-224   Language：Japanese   Book type：Scholarly book

    Repository Public URL： http://hdl.handle.net/2324/1001482699

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Presentations

  • DNAから探る霧島神宮スギ御神木と九州の神宮に残る古木の関係

    渡辺敦史・弓削直樹・田村美帆

    山岳修験学会  2023.11 

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    Event date： 2024.5

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  • ウルシ液滲出時の遺伝子発現解析 Invited

    渡辺敦史・山口莉美・田村美帆

    日本森林学会  2018.3 

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    Event date： 2018.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  • スギさし木発根における遺伝子発現プロファイルの構築 ー大量増殖手法の確立を目指してー Invited

    渡辺敦史

    2016.3 

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    Event date： 2016.5

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  • ウルシ遺伝資源評価は何をもたらすのか？蓄積された過去の財産を将来に伝えるために Invited

    渡辺敦史

    漆サミット  2020.11 

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    Event date： 2021.11 - 2020.11

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

    Country：Japan  

    日本のウルシ林の遺伝的多様性を明らかにし、渡来種とされてきたウルシが我が国にも天然分布していた可能性、日本全体のウルシ林の遺伝的関係性、育種による優良品種開発に向けた取り組み法、ウルシ林の造成・管理法などを一般市民を対象として解説している。

  • 現存ウルシ林の遺伝的多様性 Invited

    渡辺敦史・田村美帆・田端雅進

    日本森林学会  2020.3 

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    Event date： 2020.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  • 優良ウルシ選抜に向けたDNAマーカー開発の取り組み Invited

    @渡辺敦史,#山口莉美,#泉雄一郎,@田村美帆,@田端正進

    第130回日本森林学会  2019.3 

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    Event date： 2019.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  • リュウキュウマツ保全に向けた遺伝構造の解明

    渡辺敦史・田村美帆・玉城雅範

    亜熱帯研究会  2017.8 

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    Event date： 2018.8 - 2018.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  • ゲノム情報を利用したスギ育種の可能性と今後の展望 Invited

    渡辺敦史

    九州紙パ研究会  2015.6 

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    Event date： 2016.5

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：九州大学   Country：Japan  

  • ウルシ優良個体選抜に向けて Invited

    渡辺敦史

    2015.12 

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    Event date： 2016.5

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  • DNAマーカーに基づいた次世代優良少花粉スギの効率的スクリーニング �

    柳原尚貴, 坪村美代子, 平岡裕一郎, 栗田学, 平尾知士, 高橋誠, 渡辺敦史

    日本森林学会  2015.3 

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    Language：Japanese  

    Venue：北海道大学   Country：Japan  

  • 少花粉スギ品種の選抜とその特性 Invited

    坪村美代子, 中村博一, 市村よし子, 伊藤美和子, 原口雅人, 斉藤央嗣, 渡辺 敦史

    日本森林学会  2013.3 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：岩手大学   Country：Japan  

  • 適正な系統管理に向けたラベルシステムの高度化

    小野雅子, 栗田裕子, 渡辺 敦史

    森林遺伝育種学会  2012.11 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：東京大学   Country：Japan  

  • スギ次世代化に向けた材質形質の遺伝性評価 Invited

    井城泰一, 三嶋賢太郎, 石栗太, 平岡裕一郎, 三浦真弘, 渡辺 敦史

    日本森林学会  2013.3 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：岩手大学   Country：Japan  

  • スギ早期選抜にむけた EST 情報の収集と網羅的発現解析 Invited

    三嶋賢太郎, 井城泰一, 藤原健, 黒田克史, 渡辺 敦史

    日本森林学会  2013.3 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  • マツノザイセンチュウの増殖と病原性の関係

    小林玄, 松永孝治, 渡辺敦史

    森林遺伝育種学会  2014.11 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：東京大学   Country：Japan  

  • マツノザイセンチュウのクロマツ樹体内における移動と発病の関係性

    山口莉未, 平尾知士, 渡辺敦史

    森林遺伝育種学会  2014.11 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：東京大学   Country：Japan  

  • 大規模に集積されたスギ遺伝子のインフォマティクス解析

    田村美帆, 郷田乃真人, 福田有樹, 坪村美代子, 三嶋賢太郎, 渡辺敦史

    森林遺伝育種学会  2014.11 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：東京大学   Country：Japan  

  • 海岸林の未来を語る対話の場　—マツ林保護と再生に向けてー Invited

    渡辺敦史

    日本造園学会  2014.5 

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    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Venue：西日本短期大学   Country：Japan  

  • クロマツ多様性に基づく抵抗性品種の再評価 �

    宮田翔介, 岩泉正和, 井城泰一, 田村美帆, 渡辺敦史

    日本森林学会  2015.3 

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    Language：Japanese  

    Venue：北海道大学   Country：Japan  

  • 次世代抵抗性品種開発に向けたマツノザイセンチュウの再収集と遺伝資源評価

    小林玄, 松永浩治, 井城泰一, 山口莉未, 田村美帆, 渡辺敦史

    日本森林学会  2015.3 

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    Language：Japanese  

    Venue：北海道大学   Country：Japan  

  • 環境シグナルに対するスギ木部形成遺伝子群の発現応答 �

    福田有樹, 三嶋賢太郎, 田村美帆, 渡辺敦史

    日本森林学会  2015.3 

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    Language：Japanese  

    Country：Japan  

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MISC

  • ウルシ林の遺伝資源管理の実態と品種改良に向けて

    渡辺敦史

    森林科学   2021.10

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  • ウルシ林の遺伝的多様性評価と遺伝資源管理 Reviewed

    渡辺敦史

    森林技術   2020.12

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  • 分子生物学的手法を利用した次世代マツノザイセンチュウ抵抗性品種開発の取り組み

    渡辺敦史

    2014.4

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  • DNAマーカーの概要と林木育種への適用(2)DNAマーカーによる個体識別

    渡辺敦史

    北海道の林木育種 56(1), 13-17, 2013   2013.6

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  • Erratum: Correction: Evaluation of thermoregulation of different pine organs in early spring and estimation of heat reward for the western conifer seed bug (Leptoglossus occidentalis) on male cones (PloS one (2022) 17 8 DOI: 10.1371/journal.pone.0272565)

    Kitajima R., Matsuda O., Matsunaga K., Hara R., Watanabe A., Kume A.

    PloS one   19 ( 6 )   2024

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    Publisher：PloS one  

    [This corrects the article DOI: 10.1371/journal.pone.0272565.].

    DOI： 10.1371/journal.pone.0305374

    Scopus

  • 熊本県合志市におけるマツヘリカメムシの個体群動態

    原亮太朗, 松永孝治, 渡辺敦史, 細川貴弘, 久米篤

    日本森林学会大会学術講演集   134th   2023   （ ISSN:2187-6576 ）

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  • マツヘリカメムシの春の行動様式

    久米篤, 北嶋諒太郎, 松田修, 松永孝治, 原亮太郎, 渡辺敦史

    日本森林学会大会学術講演集   134th   2023   （ ISSN:2187-6576 ）

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  • マツヘリカメムシがクロマツ種子生産に及ぼす潜在的な影響

    松永孝治, 岩泉正和, 久保田正裕, 原亮太朗, 北嶋諒太郎, 細川貴弘, 渡辺敦史, 久米篤

    日本森林学会大会学術講演集   134th   2023   （ ISSN:2187-6576 ）

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  • 気候の異なる３つの植栽地におけるスギクローンの遺伝子発現の年周性の違い

    能勢 美峰, 花岡 創, 武津 英太郎, 栗田 学, 三浦 真弘, 平岡 裕一郎, 井城 泰一, 三嶋 賢太郎, 高橋 誠, 渡辺 敦史

    日本森林学会大会発表データベース   2022.5

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    Changes in annual transcriptome dynamics of Japanese cedar planted under different climate conditions
    [in Japanese]

    DOI： 10.11519/jfsc.133.0_424

  • スギ精英樹におけるゲノムワイドアソシエーション解析とゲノミック予測

    平岡裕一郎, 平尾知士, 三嶋賢太郎, 田村美帆, 武津英太郎, 井城泰一, 坪村美代子, 能勢美峰, 大平峰子, 花岡創, 高橋誠, 星比呂志, 渡辺敦史

    日本森林学会大会学術講演集   2016.3

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    スギ精英樹におけるゲノムワイドアソシエーション解析とゲノミック予測

    DOI： 10.11519/jfsc.127.0_282

  • スギさし木苗の根系成長におけるクローン間変異

    大平峰子, 花岡創, 平岡裕一郎, 渡辺敦史

    日本森林学会大会学術講演集   2016.3

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    スギさし木苗の根系成長におけるクローン間変異
    [in Japanese]

    DOI： 10.11519/jfsc.127.0_285

  • DNAマーカーに基づいた次世代優良少花粉スギの効率的スクリーニング

    柳原尚貴, 坪村美代子, 平岡裕一郎, 栗田学, 平尾知士, 高橋誠, 渡辺敦史

    日本森林学会大会学術講演集   2015.3

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    DNAマーカーに基づいた次世代優良少花粉スギの効率的スクリーニング
    [in Japanese]

    DOI： 10.11519/jfsc.126.0_306

  • 共通系統を用いたスギ初期成長の産地間差の解析

    三浦真弘, 花岡創, 平岡裕一郎, 井城泰一, 磯田圭哉, 武津英太郎, 高橋誠, 渡辺敦史

    日本森林学会大会学術講演集   2015.3

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    共通系統を用いたスギ初期成長の産地間差の解析
    [in Japanese]

    DOI： 10.11519/jfsc.126.0_322

  • スギ精英樹クローンの苗畑における初期成長の評価

    井城泰一, 大平峰子, 花岡創, 高島有哉, 平岡裕一郎, 渡辺敦史

    日本森林学会大会学術講演集   2015.3

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    スギ精英樹クローンの苗畑における初期成長の評価
    [in Japanese]

    DOI： 10.11519/jfsc.126.0_321

  • スギの材質・成長形質に関するQTL解析

    三嶋賢太郎, 平尾知士, 田村美帆, 井城泰一, 平岡裕一郎, 能勢美峰, 坪村美代子, 花岡創, 藤原健, 黒田克史, 栗田学, 高橋誠, 渡辺敦史

    日本森林学会大会学術講演集   2015.3

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    スギの材質・成長形質に関するQTL解析
    [in Japanese]

    DOI： 10.11519/jfsc.126.0_307

  • スギのさし木発根性の季節変動とその要因

    大平峰子, 花岡創, 平岡裕一郎, 栗田学, 渡辺敦史

    日本森林学会大会学術講演集   2015.3

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    スギのさし木発根性の季節変動とその要因
    [in Japanese]

    DOI： 10.11519/jfsc.126.0_335

  • 環境因子を考慮したスギさし木クローンの成長解析

    平岡裕一郎, 光田靖, 井城泰一, 花岡創, 武津英太郎, 三浦真弘, 渡辺敦史

    日本森林学会大会学術講演集   2015.3

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    環境因子を考慮したスギさし木クローンの成長解析
    [in Japanese]

    DOI： 10.11519/jfsc.126.0_337

  • DNAマーカーの概要と林木育種への適用(４)SSR vs SNP

    田村美帆, 渡辺敦史

    2014.12

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  • 針葉樹の葉緑体ゲノムを対象とした最近の研究動向

    平尾知士, 渡辺敦史

    2014.7

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  • 優良な形質を併せ持つ雄性不稔スギの作出―雄性不稔スギリソース整備に向けて―

    坪村美代子, 平岡裕一郎, 栗田学, 高橋誠, 渡辺敦史

    日本森林学会大会学術講演集(CD-ROM)   2014.3

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    優良な形質を併せ持つ雄性不稔スギの作出―雄性不稔スギリソース整備に向けて―
    [in Japanese]

    DOI： 10.11519/jfsc.125.0_719

  • DNAマーカーの概要と林木育種への適用①　PCRとDNAマーカーの種類

    渡辺 敦史

    北海道の林木の育種、55：28-32   2013.2

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  • Extended linkage disequilibrium in non-coding regions in a conifer, Cryptomeria japonica

    Ryoshiro Munehisa, Yosuke Hisataka, Etsuko Moritsuka, Kentaro Uchiyama, Atsushi Watanabe, Yoshihiko Tsumura, Hidenori Tachida

    GENES & GENETIC SYSTEMS   2012.12

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  • Extended linkage disequilibrium in noncoding regions in a conifer, Cryptomeria Japonica

    Etsuko Moritsuka, Yosuke Hisataka, Miho Tamura, Kentaro Uchiyama, Atsushi Watanabe, Yoshihiko Tsumura, Hidenori Tachida

    Genetics   2012.3

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    Language：Others  

    We measured linkage disequilibrium in mostly noncoding regions of Cryptomeria japonica, a conifer belonging to Cupressaceae. Linkage disequilibrium was extensive and did not decay even at a distance of 100 kb. The average estimate of the population recombination rate per base pair was 1.55×10 -5 and was <1/70 of that in the coding regions. We discuss the impact of low recombination rates in a large part of the genome on association studies. © 2012 by the Genetics Society of America.

    DOI： 10.1534/genetics.111.136697

  • Extended linkage disequilibrium in non-coding regions in a conifer, Cryptomeria japonica

    Yosuke Hisataka, Etsuko Moritsuka, Kentaro Uchiyama, Atsushi Watanabe, Yoshihiko Tsumura, Hidenori Tachida

    GENES & GENETIC SYSTEMS   2011.12

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  • マツノザイセンチュウ抵抗性品種開発の最新研究

    渡辺 敦史

    グリーンエイジ，36（６）: 17-20   2009.6

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  • 現代における葉緑体ゲノム研究の動向

    平尾知士, 渡辺 敦史

    林木の育種，229，6-10   2008.10

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  • 福島県におけるマツノザイセンチュウ抵抗性育種事業のこれから

    小澤創, 渡邊次郎, 陳宏玲, 渡辺 敦史

    林木の育種，217 : 1-5   2006.4

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  • 分子系統地理学的解析を用いた宮城県内におけるブナ苗木の流通許容範囲の推定

    菅野学, 陶山佳久, 原正利, 高橋誠, 渡邊敦史, 清和研二

    日本森林学会大会学術講演集(CD-ROM)   2006.4

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    分子系統地理学的解析を用いた宮城県内におけるブナ苗木の流通許容範囲の推定

    DOI： 10.11519/jfsc.117.0.31.0

  • 林木育種におけるDNAマーカーの適用

    渡辺 敦史

    林木の育種，216：7-13   2005.7

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  • 従来の育種技術とバイオテクノロジー等新技術との統合による新たな林木育種の展開：マイクロサテライトマーカーの開発と利用

    渡辺 敦史

    森林科学，41: 44-46   2004.6

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  • Discrimination of teak (Tectona grandis) plus trees using selected random amplified polymorphic DNA (RAPD) markers

    A. Watanabe, A. Widyatmoko, A. Rimbawanto, S. Shiraishi

    Journal of Tropical Forest Science   2004.1

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    To achieve a highly reliable clone management of teak (Tectona grandis) plus trees, useful DNA molecular markers were surveyed using RAPD analysis and their ability to discriminate among plus tree clones was examined. A primary screening was performed for 120 arbitrary decamer primers, and 24 primers that generated 26 clear and unambiguous fragments were selected. In the secondary screening, the reproducibility of each fragment was investigated by six repetitions of polymerase chain reaction, and 13 fragments found to be most reproducible were finally selected. Evaluation of the discriminatory powers of these fragments suggested that the selected RAPD markers would be useful in the clone management of teak plus trees.

  • DNA情報に基づくマツ属の分類と系統進化

    渡辺 敦史

    林木の育種，184：31-34   1997.7

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  • DNAがあかすアイノコマツの正体

    白石 進, 渡辺 敦史, 那須仁弥, 戸田忠雄

    林木の育種，183：１-6   1997.4

    　More details

    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  • 分類困難な日本産針葉樹のDNA分類・系統学的研究

    白石 進, 渡辺 敦史, 磯田圭哉

    森林文化研究，17：123-132   1996.4

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

  • 隔離分布する天然生北限カラマツの特性と保存. DNAが語る馬の神岳カラマツの生い立ち

    白石 進, 渡辺 敦史, 磯田圭哉, 河崎久男

    林木の育種，177：1-5   1995.10

    　More details

    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

▼display all

Works

  • 二次元バーコードラベルシステム　もばらべ

    渡辺敦史, 栗田裕子, 小野雅子（森林総合研究所林木育種センター）, 株式会社サトー

    2012.9

  • 樹木個体管理システム

    渡辺敦史 小野雅子・高橋誠（森林総合研究所林木育種センター） 竹谷商事

    2012.8

Professional Memberships

  • 根学会

    2024.6 - Present

  • 九州森林学会

  • 日本木材学会

  • 森林遺伝育種学会

  • 日本育種学会

  • 日本森林学会

  • 樹木医学会

  • 園芸学会

  • 山岳修験学会

▼display all

Committee Memberships

  • 九州森林学会   編集幹事   Domestic

    2025.4 - 2026.3   

  • 九州森林学会   編集幹事   Domestic

    2022.4 - 2024.3   

  • 森林遺伝育種学会   編集委員長   Domestic

    2018.4 - 2022.3   

  • 樹木医学会   Organizer   Domestic

    2018.4 - 2019.6   

  • 樹木医学会   第23回樹木医学会大会事務局長   Domestic

    2018.4 - 2019.6   

  • 生物遺伝資源委員会（GRC   委員   Domestic

    2016.11 - Present   

  • 九州森林学会   Organizer   Domestic

    2012.10 - 2014.9   

  • 九州森林学会   常任幹事   Domestic

    2012.10 - 2014.9   

  • 森林遺伝育種学会   Executive   Domestic

    2012.4 - 2022.3   

  • 日本育種学会   編集委員   Domestic

    2011.6 - Present   

▼display all

Academic Activities

  • Screening of academic papers

    Role(s)： Peer review

    2023

    　More details

    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：5

  • 九州森林学会

    2022.4 - 2024.3

    　More details

    Type：Academic society, research group, etc. 

  • 司会

    第10回森林遺伝育種学会シンポジウム  （ Japan ） 2021.3

    　More details

    Type：Competition, symposium, etc. 

  • コーディネーター

    第132回日本森林学会大会シンポジウム  （ Japan ） 2021.3

    　More details

    Type：Competition, symposium, etc. 

  • Screening of academic papers

    Role(s)： Peer review

    2021

    　More details

    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：10

    Number of peer-reviewed articles in Japanese journals：2

  • コーディネーター

    第9回森林遺伝育種学会シンポジウム  （ Japan ） 2020.11

    　More details

    Type：Competition, symposium, etc. 

  • Screening of academic papers

    Role(s)： Peer review

    2020

    　More details

    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：15

  • コーディネーター

    第130回日本森林学会  （ Japan ） 2019.3

    　More details

    Type：Competition, symposium, etc. 

  • コーディネーター

    第8回森林遺伝育種学会シンポジウム  （ Japan ） 2019.3

    　More details

    Type：Competition, symposium, etc. 

  • Screening of academic papers

    Role(s)： Peer review

    2019

    　More details

    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：17

  • コーディネーター

    第23回樹木医学会  （ Japan ） 2018.4 - 2019.3

    　More details

    Type：Competition, symposium, etc. 

  • 森林遺伝育種学会

    2018.4 - 2022.3

    　More details

    Type：Academic society, research group, etc. 

  • コーディネーター

    日本森林学会  （ Japan ） 2018.3

    　More details

    Type：Competition, symposium, etc. 

  • Screening of academic papers

    Role(s)： Peer review

    2018

    　More details

    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：17

    Number of peer-reviewed articles in Japanese journals：2

  • Screening of academic papers

    Role(s)： Peer review

    2017

    　More details

    Type：Peer review 

    Number of peer-reviewed articles in foreign language journals：8

    Number of peer-reviewed articles in Japanese journals：1

  • 座長（Chairmanship）

    九州森林学会  （ Japan ） 2016.11

    　More details

    Type：Competition, symposium, etc. 

  • コーディネーター

    日本森林学会企画シンポジウム林木育種において環境適応とどう向き合うか？－ゲノム、遺伝子発現及び表現型解析からの新たな知見－  （ Japan ） 2016.3

    　More details

    Type：Competition, symposium, etc. 

    Number of participants：100

  • 司会（Moderator）

    日本森林学会  （ Japan ） 2015.3

    　More details

    Type：Competition, symposium, etc. 

  • コーディネーター

    日本森林学会テーマ別セッション　マツ材線虫病研究最近の進歩　－オミクス的展開による挑戦－  （ Japan ） 2015.3

    　More details

    Type：Competition, symposium, etc. 

    Number of participants：50

  • 座長（Chairmanship）

    九州森林学会  （ Japan ） 2014.10

    　More details

    Type：Competition, symposium, etc. 

  • コーディネーター

    日本森林学会  （ Japan ） 2014.3 - 2014.5

    　More details

    Type：Competition, symposium, etc. 

  • コーディネーター

    日本森林学会テーマ別セッション　大規模形質データと分子データの統合  （ Japan ） 2014.3

    　More details

    Type：Competition, symposium, etc. 

    Number of participants：100

  • コーディネーター

    日本森林学会テーマ別セッション　林木育種による花粉症対策 -その成果と検証-  （ Japan ） 2013.3

    　More details

    Type：Competition, symposium, etc. 

    Number of participants：60

  • コーディネーター

    日本森林学会テーマ別セッション　日本の材質育種研究の近未来予想図  （ Japan ） 2013.3

    　More details

    Type：Competition, symposium, etc. 

    Number of participants：150

  • 座長（Chairmanship）

    九州森林学会  （ Japan ） 2012.10

    　More details

    Type：Competition, symposium, etc. 

  • 講演

    漆サミット in 浄法寺  （ Japan ） 2012.10

    　More details

    Type：Competition, symposium, etc. 

    Number of participants：150

  • 日本育種学会

    2009.4 - Present

    　More details

    Type：Academic society, research group, etc. 

▼display all

Research Projects

  • Artistic and Cultural Practices to Regenerate the Relationship between the Forest Environment and Human Beings after a Disaster: Focusing on the Hikosan Watershed

    Grant number：24K00031  2024.4 - 2028.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    知足 美加子, 渡辺 敦史, 石上 洋明, 清野 聡子, 加藤 悠希

    　 More details

    Grant type：Scientific research funding

    本研究は、災害被災地の森林環境と人間の関係性を再生するファクターとして芸術文化に着目する。芸術文化による「体験」を通じた精神的価値の創出が、災害復興への行動を喚起する可能性を検証する。具体的には1.全国の被災地における復興と芸術文化の関係についての事例研究。2.英彦山修験道や朝倉三連水車等の治水に関する伝統知の発掘と、森林の遺伝的系統の科学的調査に基づく新たな価値創造。3.上記を踏まえデジタル技術と彫刻技術を組み合わせた芸術文化的実践を行う。森林環境と人間の関係性を育み、自然の再生力と持続性を可視化する創造的な取り組みになることが期待される。

    CiNii Research

  • マツ材線虫病の萎凋枯死メカニズムの全容解明

    Grant number：21H04734  2021.4 - 2025.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    　 More details

    Grant type：Scientific research funding

  • 新規さし木手法がもたらすさし木発根誘導シグナルの特定

    2021.4 - 2024.3

    　 More details

    Authorship：Principal investigator  Grant type：Scientific research funding

  • くるめつつじのDNA分析調査

    2019.4 - 2021.3

    Research commissions

    　 More details

    Authorship：Principal investigator  Grant type：Other funds from industry-academia collaboration

  • シグナル物質の作用機序とラッカーゼの構造解析による高品質漆生成技術の開発

    Grant number：19H00551  2019 - 2022

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

    　 More details

    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

  • 日本の漆文化を継承する国産漆の増産、改質・利用技術の開発

    2018.4 - 2019.3

    　 More details

    Authorship：Coinvestigator(s) 

  • 九州北部豪雨による流木被害の要因と影響：森林環境政策の合意形成に向けて

    Grant number：18H04152  2018 - 2021

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    　 More details

    Grant type：Scientific research funding

  • 用土を用いない空中さし木法による、コスト３割削減で２倍の生産量を実現するスギ

    2018 - 2020

    農林水産省　イノベーション創出強化研究推進事業

    　 More details

    Authorship：Coinvestigator(s)  Grant type：Contract research

  • 人文社会学的解析と森林遺伝学的解析に基づく国内クロマツ松原の歴史的造成プロセスの解明

    2017.10 - 2019.3

    　 More details

    Authorship：Coinvestigator(s) 

  • 九州のスギの起源を探る－系譜情報に基づく九州スギ遺伝的リソースの成り立ちの解明－

    Grant number：17K07857  2017 - 2019

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    　 More details

    Grant type：Scientific research funding

  • 現存有名クロマツ植栽林の遺伝的保全のための採種戦略の確立

    Grant number：17K07853  2017 - 2019

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    　 More details

    Grant type：Scientific research funding

  • 熊本県産ヒノキ品種南郷檜の管理・生産手法の効率化

    2016.7 - 2017.3

    Research commissions

    　 More details

    Authorship：Principal investigator  Grant type：Other funds from industry-academia collaboration

  • 気候変動に適応した花粉発生源対策スギの作出技術開発

    2016.4 - 2020.3

    Joint research

    　 More details

    Authorship：Coinvestigator(s)  Grant type：Other funds from industry-academia collaboration

  • リュウキュウマツの遺伝的多様性と進化的位置

    2016.4 - 2019.3

    　 More details

    Authorship：Principal investigator 

    島嶼群に点在するリュウキュウマツの進化的位置と遺伝的多様性に関する固有性について明らかにする

  • 日本の漆文化を継承する国産漆の増産、改質・利用技術の開発

    2016.4 - 2018.3

    Joint research

    　 More details

    Authorship：Coinvestigator(s)  Grant type：Other funds from industry-academia collaboration

  • リュウキュウマツの遺伝的多様性と進化的位置の解明

    Grant number：16K07780  2016 - 2018

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    　 More details

    Authorship：Principal investigator  Grant type：Scientific research funding

  • マツノザイセンチュウ抵抗性品種開発高度化事業

    2015.4 - 2016.3

    　 More details

    Authorship：Coinvestigator(s) 

  • ウルシ生産量増大に向けた諸要因の解明

    2014.5 - 2016.3

    　 More details

    Authorship：Coinvestigator(s) 

    Identification genes related to sap flow in Toxicodendron species

  • 漆生成メカニズムに基づく高品質漆の開発

    Grant number：26242017  2014 - 2017

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

    　 More details

    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

  • 革新的に高速化した新世代林業種苗選抜技術の開発

    2012.4 - 2016.3

    Joint research

    　 More details

    Authorship：Coinvestigator(s)  Grant type：Other funds from industry-academia collaboration

  • スギ木部形成関連遺伝子データベースの構築に向けたEST単離と網羅的発現解析

    2012.4 - 2014.3

    　 More details

    Authorship：Coinvestigator(s) 

  • スギ根系の構造および成長特性に及ぼす遺伝的要因の解明

    Grant number：24780160  2012 - 2016

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    　 More details

    Grant type：Scientific research funding

  • スギ木部形成関連遺伝子データベースの構築に向けたEST単離と網羅的発現解析

    2012 - 2014

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Specially Promoted Research

    　 More details

    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

  • スギ木部形成関連遺伝子データベースの構築に向けたＥＳＴ単離と網羅的発現解析

    Grant number：24380098  2012 - 2014

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    　 More details

    Grant type：Scientific research funding

  • 地域活性化を目指した国産ウルシの持続的管理・生産技術の開発

    2011.4 - 2013.3

    Joint research

    　 More details

    Authorship：Coinvestigator(s)  Grant type：Other funds from industry-academia collaboration

  • 生体分子の相互作用に基づいたマツ材線虫病発病機構の解明

    Grant number：23248024  2011 - 2013

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    　 More details

    Grant type：Scientific research funding

  • エリシター受容体遺伝子をターゲットにしたマツノザイセンチュウ抵抗性遺伝子の単離

    Grant number：23780170  2011 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    　 More details

    Grant type：Scientific research funding

  • 花粉症対策ヒノキ・スギ品種の普及拡大技術開発と雄性不稔品種開発

    2010.4 - 2014.3

    Joint research

    　 More details

    Authorship：Principal investigator  Grant type：Other funds from industry-academia collaboration

  • スギの花形成に関与するジベレリン生合成関連遺伝子の同定

    Grant number：23780173  2010 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    　 More details

    Grant type：Scientific research funding

  • スギ精英樹の形質データの再測定及び次代検定林データの収集とりまとめ

    2009.4 - 2014.3

    Joint research

    　 More details

    Authorship：Coinvestigator(s)  Grant type：Other funds from industry-academia collaboration

  • スギ精英樹の形質データの再測定及び次代検定林データの収集とりまとめ

    2009.4 - 2014.3

    森林総合研究所（日本） 

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    Authorship：Coinvestigator(s) 

  • 核およびオルガネラDNA情報に基づくスギの系統地理学的研究

    Grant number：21380102  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    　 More details

    Grant type：Scientific research funding

  • マツノザイセンチュウ抵抗性候補遺伝子のマッピングとeQTL解析

    Grant number：21780157  2009 - 2010

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    　 More details

    Grant type：Scientific research funding

  • 比較ゲノムを利用したマツ属植物からのマツノザイセンチュウ抵抗性遺伝子の単離

    Grant number：21780156  2009 - 2010

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    　 More details

    Grant type：Scientific research funding

  • 無花粉スギと精英樹の人工交配による新品種の開発とその早期判定技術の開発

    2008.4 - 2013.3

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    Authorship：Principal investigator  Grant type：Other funds from industry-academia collaboration

  • スギ雄花着花量を制御する遺伝子の解明

    Grant number：20380096  2008 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    　 More details

    Grant type：Scientific research funding

  • マツノザイセンチュウに対する抵抗性マツの抵抗性発現機構の解明

    Grant number：20380085  2008 - 2010

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    　 More details

    Grant type：Scientific research funding

  • 3葉緑体ゲノムのSNPを用いたブナの分子系統地理学的・環境適応的研究

    2007 - 2009

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    　 More details

    Authorship：Coinvestigator(s)  Grant type：Scientific research funding

  • 葉緑体ゲノムのSNPを用いたブナの分子系統地理学的・環境適応的研究

    Grant number：19380096  2007 - 2009

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    　 More details

    Grant type：Scientific research funding

  • マツノザイセンチュウに対するマツの生体防御関連遺伝子の探索と発現定量解析

    Grant number：19580182  2007 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    　 More details

    Grant type：Scientific research funding

  • マツノザイセンチュウ抵抗性品種の開発

    2006.4 - 2009.3

    Research commissions

    　 More details

    Authorship：Principal investigator  Grant type：Other funds from industry-academia collaboration

  • マツESTデータベースを利用したSNP探索とSNPマーカーの開発

    2005 - 2006

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    　 More details

    Authorship：Principal investigator  Grant type：Scientific research funding

  • マツESTデータベースを利用したSNP探索とSNPマーカーの開発

    2005 - 2006

    Global COE Program (Ministry of Education, Culture, Sports, Science and Technology)

    　 More details

    Grant type：Contract research

  • 林木の生殖・繁殖特性の評価に関する研究

    2003.4 - 2005.3

    Research commissions

    　 More details

    Authorship：Principal investigator  Grant type：Other funds from industry-academia collaboration

  • 染色体地図情報に基づくマツ属植物のゲノム進化解明

    1999 - 2001

    Global COE Program (Ministry of Education, Culture, Sports, Science and Technology)

    　 More details

    Grant type：Contract research

▼display all

Educational Activities

  • ［学部教育］
    植物代謝制御学
    造林学
    森林植物学
    森林植物学実験
    Forest product Science　（国際コース）
    農学入門（基幹教育）
    卒業研究

    ［大学院教育］
    森林・林業の科学
    森林資源管理学
    造林学特論
    森林環境科学プロジェクト演習
    森林環境科学特別研究第一・第二

Class subject

  • 森林環境科学演習第一

    2024.10 - 2025.3   Second semester

  • 森林環境科学演習第一

    2024.10 - 2025.3   Second semester

  • 森林植物学

    2024.10 - 2024.12   Fall quarter

  • 造林学特論

    2024.10 - 2024.12   Fall quarter

  • Forest Products Science Ⅱ

    2024.6 - 2024.8   Summer quarter

  • 修士論文

    2024.4 - 2025.3   Full year

  • 森林環境科学プロジェクト演習

    2024.4 - 2025.3   Full year

  • 森林植物学実験

    2024.4 - 2024.9   First semester

  • 科学英語（森林機能開発学分野）

    2024.4 - 2024.9   First semester

  • 造林学Ⅰ

    2024.4 - 2024.6   Spring quarter

  • Forest Products Science Ⅰ

    2024.4 - 2024.6   Spring quarter

  • 森林環境科学演習第一

    2023.10 - 2024.3   Second semester

  • 森林環境科学演習第二

    2023.10 - 2024.3   Second semester

  • 造林学特論

    2023.10 - 2023.12   Fall quarter

  • 基幹教育セミナー

    2023.6 - 2023.8   Summer quarter

  • 造林学Ⅱ

    2023.6 - 2023.8   Summer quarter

  • 森林環境科学プロジェクト演習

    2023.4 - 2024.3   Full year

  • 修士論文

    2023.4 - 2024.3   Full year

  • 森林環境科学特別研究第二

    2023.4 - 2024.3   Full year

  • 森林環境科学特別研究第一

    2023.4 - 2024.3   Full year

  • 森林・林業の科学

    2023.4 - 2023.9   First semester

  • 科学英語（森林機能開発学分野）

    2023.4 - 2023.9   First semester

  • 森林・林業の科学(国際コース)

    2023.4 - 2023.6   Spring quarter

  • Forest Products Science

    2023.4 - 2023.6   Spring quarter

  • 造林学Ⅰ

    2023.4 - 2023.6   Spring quarter

  • 農学入門Ⅰ

    2022.10 - 2023.3   Second semester

  • 森林環境科学演習第二

    2022.10 - 2023.3   Second semester

  • 森林環境科学演習第一

    2022.10 - 2023.3   Second semester

  • 造林学特論

    2022.10 - 2022.12   Fall quarter

  • 造林学Ⅱ

    2022.6 - 2022.8   Summer quarter

  • 農学入門

    2022.4 - 2023.3   Full year

  • 修士論文

    2022.4 - 2023.3   Full year

  • 森林環境科学特別研究第二

    2022.4 - 2023.3   Full year

  • 森林環境科学特別研究第一

    2022.4 - 2023.3   Full year

  • 森林環境科学プロジェクト演習

    2022.4 - 2023.3   Full year

  • 科学英語（森林機能開発学分野）

    2022.4 - 2022.9   First semester

  • Forest Products Science

    2022.4 - 2022.6   Spring quarter

  • 造林学Ⅰ

    2022.4 - 2022.6   Spring quarter

  • 森林・林業の科学(国際コース)

    2022.4 - 2022.6   Spring quarter

  • 森林・林業の科学

    2022.4 - 2022.6   Spring quarter

  • 森林資源管理学

    2022.4 - 2022.6   Spring quarter

  • 樹木生理学特論

    2021.12 - 2022.2   Winter quarter

  • 造林学特論

    2021.10 - 2021.12   Fall quarter

  • 農学入門

    2021.4 - 2022.3   Full year

  • 造林学

    2021.4 - 2021.9   First semester

  • 植物代謝制御学

    2021.4 - 2021.9   First semester

  • 森林植物学実験

    2021.4 - 2021.9   First semester

  • 森林・林業の科学

    2021.4 - 2021.6   Spring quarter

  • Forest Products Science

    2021.4 - 2021.6   Spring quarter

  • 森林資源管理学

    2021.4 - 2021.6   Spring quarter

  • 樹木生理学特論

    2020.12 - 2021.2   Winter quarter

  • 造林学特論

    2020.10 - 2020.12   Fall quarter

  • 基幹教育セミナー

    2020.6 - 2020.8   Summer quarter

  • 農学入門

    2020.4 - 2021.3   Full year

  • 造林学

    2020.4 - 2020.9   First semester

  • 植物代謝制御学

    2020.4 - 2020.9   First semester

  • 森林植物学実験

    2020.4 - 2020.9   First semester

  • 森林・林業の科学

    2020.4 - 2020.6   Spring quarter

  • Forest Products Science

    2020.4 - 2020.6   Spring quarter

  • 森林資源管理学

    2020.4 - 2020.6   Spring quarter

  • 樹木生理学特論

    2019.12 - 2020.2   Winter quarter

  • 森林遺伝育種学

    2019.10 - 2020.3   Second semester

  • 造林学特論

    2019.10 - 2019.12   Fall quarter

  • 農学入門

    2019.4 - 2020.3   Full year

  • 造林学

    2019.4 - 2019.9   First semester

  • 植物代謝制御学

    2019.4 - 2019.9   First semester

  • 森林植物学実験

    2019.4 - 2019.9   First semester

  • 森林資源管理学

    2019.4 - 2019.6   Spring quarter

  • 森林・林業の科学

    2019.4 - 2019.6   Spring quarter

  • Forest Products Science

    2019.4 - 2019.6   Spring quarter

  • 樹木生理学特論

    2018.12 - 2019.2   Winter quarter

  • 森林遺伝育種学

    2018.10 - 2019.3   Second semester

  • 農学入門

    2018.4 - 2019.3   Full year

  • 造林学

    2018.4 - 2018.9   First semester

  • 植物代謝制御学

    2018.4 - 2018.9   First semester

  • 森林植物学実験

    2018.4 - 2018.9   First semester

  • 造林学特論

    2018.4 - 2018.9   First semester

  • 森林・林業の科学

    2018.4 - 2018.6   Spring quarter

  • Forest Products Science

    2018.4 - 2018.6   Spring quarter

  • 森林資源管理学

    2018.4 - 2018.6   Spring quarter

  • 森林植物科学

    2017.10 - 2018.3   Second semester

  • 森林遺伝育種学

    2017.10 - 2018.3   Second semester

  • 農学入門

    2017.4 - 2018.3   Full year

  • 植物代謝制御学

    2017.4 - 2017.9   First semester

  • 造林学

    2017.4 - 2017.9   First semester

  • 森林植物学実験

    2017.4 - 2017.9   First semester

  • 造林学特論

    2017.4 - 2017.9   First semester

  • Forest Products Science

    2017.4 - 2017.6   Spring quarter

  • 森林植物科学

    2016.10 - 2017.3   Second semester

  • 森林遺伝育種学

    2016.10 - 2017.3   Second semester

  • 農学入門

    2016.4 - 2017.3   Full year

  • 造林学特論

    2016.4 - 2016.9   First semester

  • 森林植物科学実験

    2016.4 - 2016.9   First semester

  • 造林学

    2016.4 - 2016.9   First semester

  • Forest Product Science

    2016.4 - 2016.9   First semester

  • 植物代謝制御学

    2016.4 - 2016.9   First semester

  • 森林遺伝育種学

    2015.10 - 2016.3   Second semester

  • 森林植物科学

    2015.10 - 2016.3   Second semester

  • 農学入門

    2015.4 - 2016.3   Full year

  • 植物代謝制御学

    2015.4 - 2015.9   First semester

  • Forest Product Science

    2015.4 - 2015.9   First semester

  • 森林植物科学

    2014.10 - 2015.3   Second semester

  • 森林遺伝育種学

    2014.10 - 2015.3   Second semester

  • 農学入門

    2014.4 - 2015.3   Full year

  • Forest Product Science

    2014.4 - 2014.9   First semester

  • 植物代謝制御学

    2014.4 - 2014.9   First semester

  • Challenges in agricultural science and technology of plants

    2013.10 - 2014.3   Second semester

  • 森林植物科学

    2013.10 - 2014.3   Second semester

  • 森林遺伝育種学

    2013.10 - 2014.3   Second semester

  • 植物代謝制御学

    2013.4 - 2013.9   First semester

  • 自然科学総合実験

    2013.4 - 2013.9   First semester

  • 森林植物科学

    2012.10 - 2013.3   Second semester

  • 森林遺伝育種学

    2012.10 - 2013.3   Second semester

▼display all

FD Participation

  • 2013.4   Role：Participation   Title：第一回全学FD

    Organizer：University-wide

Visiting, concurrent, or part-time lecturers at other universities, institutions, etc.

  • 2023  広島県立大学  Classification:Intensive course  Domestic/International Classification:Japan 

Social Activities

  • タイトル：悠久の歴史と人々の生活と共に

    特定非営利活動法人 循環生活研究所  福岡  2018.2

    　More details

    Audience：General,　Scientific,　Company,　Civic organization,　Governmental agency

    Type：Other

  • 松原と地域（人）との関わり方

    NPO　SSJ  福岡  2018.2

    　More details

    Audience：General,　Scientific,　Company,　Civic organization,　Governmental agency

    Type：Lecture

    日本の海岸クロマツ林は、江戸期からの植栽林であり、このクロマツ林によって防風・防砂が行われた結果、海岸周辺に住む人々は田畑・家等が守られてきた。しかし、外来種であるマツノザイセンチュウによって引き起こされたマツ材線虫病の蔓延は、各地でクロマツ林を壊滅させている。1970年代以降、行政・住民が団結して地域のクロマツ林を守るための努力を行ってきたが、増大する労力に対してその効果が報われることは少なく、薬剤散布に対する嫌悪感、広葉樹林に対する過度な賛美など一般市民の意識は確実に変化している。一方で、現在でも海岸マツ林を守る住民努力が行われていることも事実である。本講座では、これら地域マツ林が成立した歴史的経緯やクロマツ林が地域に与えてきた恩恵を住民に説明し、クロマツ林の有効性について認識をはかる。

  • マツ林保護と再生に向けて

    NPO　SSJ  福岡  2016.8

    　More details

    Audience：General,　Scientific,　Company,　Civic organization,　Governmental agency

    Type：Lecture

  • 熊本県小川小学校出張講義

    2015.11

    　More details

    Audience：Infants,　Schoolchildren,　Junior students,　High school students

    Type：Other

  • 生の松原再生活動

    NPO　SSJ  生の松原  2015.1

    　More details

    Audience：General,　Scientific,　Company,　Civic organization,　Governmental agency

    Type：Other

  • 出張講義

    育徳高校  2014.8

    　More details

    Audience：Infants,　Schoolchildren,　Junior students,　High school students

    Type：Seminar, workshop

▼display all

Media Coverage

  • スギ花粉症対策として進められてきたスギ雄性不稔遺伝子座を初めて特定し、新たなDNAマーカーを開発した事について新聞記事で紹介された。javascript:moveMainMenuPage('100020001',%20'01') Newspaper, magazine

    2018.2

    　More details

    スギ花粉症対策として進められてきたスギ雄性不稔遺伝子座を初めて特定し、新たなDNAマーカーを開発した事について新聞記事で紹介された。javascript:moveMainMenuPage('100020001',%20'01')

  • スギ花粉について TV or radio program

    2017.2

    　More details

    スギ花粉について

  • マツノザイセンチュウ抵抗性に関する遺伝子レベルの研究成果について Newspaper, magazine

    読売新聞  2012.3

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    マツノザイセンチュウ抵抗性に関する遺伝子レベルの研究成果について